RLG00000010294

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011017
Physical Location & Seq
Forward (+)
361942 .. 364909
2968 bp
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UTR
Exon/CDS
Intron
RLM00000010294

Sequence Viewer

Length: 486 bp
ATGGCTTGCATGTACTTCGCTAACGCCATGTCGTTCCGGCCAGTCCCATTCTCCGGCGGCGCCGGAAATTACCAGAAGCCAACCAATTTTCTTGCCAGAAGGAAGTCGGTCTCCTGCGCTGGAAGTTCGCCGGAAATCGAGCTCGAATTCATAGCGCCGAAGCCGGCGAACGACGGGTCGTATGCGGTGGAGAAGGCGACGGCGATTAGTGGGGAGAAGCTCCTGAGGAGCATAATGTCGGATAACAAGATCGAGCTTTACGCCACGTATGGGAAACTGATGAACTGTGGAGGTGTAGGAAGCTGTGGGACTTGCATTGTAGAGATTATTGATGGGAAGGATCTTTTGAATGAAAGAACAAATACTGAACTCAAATATTTGAAAAAGAAACCTGAATCTTGGAGACTAGCTTGCCAAACCATAGTGGGAAATAAAGAAAATTCTGGGAAGGTTCTTGTTCAGAGGATTCCCCAGTGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

162

Amino Acids

17.68

Weight (kDa)

9.23

Isoelectric Point (pI)

53.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 94 - 142 1.3e-06 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32590 AT4G32590 AT4G32590 AT4G32590 AT4G32590
fragaria_vesca FvH4_2g30090 FvH4_2g30090
malus_domestica MD15G1116000.v1.1
prunus_persica Prupe.1G467300_v2.0.a1
pyrus_communis pycom15g10460
rosa_chinensis RchiOBHm_Chr6g0312831
rosa_laevigata RLG00000010294
rosa_multiflora Rmu_co8502815.1_g000001
rosa_roxburghii Rroxscaffold_7G00156450
rosa_rugosa Rorug06G0399900
rosa_samantha Rh6AG517100 Rh6BG528500 Rh6CG533200 Rh6DG519700
rosa_wichuraiana Rw6G044920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AciI CCGC 2 cut(s) 57, 185
AclWI GGATC 1 cut(s) 348
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 2 cut(s) 146, 439
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 2 cut(s) 53, 270
AgsI TTSAA 2 cut(s) 349, 382
AluBI AGCT 5 cut(s) 142, 220, 256, 303, 410
AluI AGCT 5 cut(s) 142, 220, 256, 303, 410
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 115, 397
AlwI GGATC 1 cut(s) 348
AoxI GGCC 1 cut(s) 38
ApoI RAATTY 2 cut(s) 146, 439
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspLEI GCGC 3 cut(s) 62, 119, 157
AxyI CCTNAGG 1 cut(s) 224
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 144
BccI CCATC 1 cut(s) 326
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 115, 397
BfaI CTAG 1 cut(s) 407
BfoI RGCGCY 2 cut(s) 63, 158
BisI GCNGC 1 cut(s) 58
BlsI GCNGC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 61
BmrI ACTGGG 1 cut(s) 466
BmuI ACTGGG 1 cut(s) 466
BsaAI YACGTR 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 115
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 270
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 2 cut(s) 41, 472
Bse21I CCTNAGG 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 53, 270
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 2 cut(s) 41, 472
BseRI GAGGAG 1 cut(s) 241
BshFI GGCC 1 cut(s) 40
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 5 cut(s) 37, 54, 63, 131, 164
BslFI GGGAC 2 cut(s) 29, 322
BslI CCNNNNNNNGG 2 cut(s) 53, 270
BsmAI GTCTC 2 cut(s) 115, 397
BsmFI GGGAC 2 cut(s) 29, 322
BsnI GGCC 1 cut(s) 40
Bso31I GGTCTC 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 2 cut(s) 249, 340
BspACI CCGC 2 cut(s) 57, 185
BspANI GGCC 1 cut(s) 40
BspCNI CTCAG 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 61
BspPI GGATC 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 2 cut(s) 41, 472
BssAI RCCGGY 1 cut(s) 163
BssMI GATC 2 cut(s) 249, 340
BssNI GRCGYC 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 287
BstACI GRCGYC 1 cut(s) 60
BstBAI YACGTR 1 cut(s) 267
BstC8I GCNNGC 3 cut(s) 7, 165, 412
BstDEI CTNAG 1 cut(s) 224
BstH2I RGCGCY 2 cut(s) 63, 158
BstHHI GCGC 3 cut(s) 62, 119, 157
BstKTI GATC 2 cut(s) 252, 343
BstMAI GTCTC 2 cut(s) 115, 397
BstMBI GATC 2 cut(s) 249, 340
BstNSI RCATGY 1 cut(s) 13
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 224
BsuRI GGCC 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 479
Cac8I GCNNGC 3 cut(s) 7, 165, 412
CfoI GCGC 3 cut(s) 62, 119, 157
Cfr10I RCCGGY 1 cut(s) 163
Csp6I GTAC 1 cut(s) 13
CviAII CATG 2 cut(s) 10, 28
CviJI RGCY 9 cut(s) 5, 40, 79, 142, 163, 220, 256, 303, 410
CviKI_1 RGCY 9 cut(s) 5, 40, 79, 142, 163, 220, 256, 303, 410
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 1 cut(s) 224
DinI GGCGCC 1 cut(s) 61
DpnI GATC 2 cut(s) 251, 342
DpnII GATC 2 cut(s) 249, 340
EaeI YGGCCR 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 142
Eco81I CCTNAGG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 142
EcoRI GAATTC 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 144
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
FaeI CATG 2 cut(s) 13, 31
FaiI YATR 7 cut(s) 11, 29, 152, 183, 233, 270, 422
FaqI GGGAC 2 cut(s) 29, 322
FatI CATG 2 cut(s) 9, 27
Fnu4HI GCNGC 1 cut(s) 58
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 58
FspBI CTAG 1 cut(s) 407
GlaI GCGC 3 cut(s) 61, 118, 156
GluI GCNGC 1 cut(s) 58
HaeII RGCGCY 2 cut(s) 63, 158
HaeIII GGCC 1 cut(s) 40
HapII CCGG 5 cut(s) 37, 54, 63, 131, 164
HhaI GCGC 3 cut(s) 62, 119, 157
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 2 cut(s) 13, 31
Hin6I GCGC 3 cut(s) 60, 117, 155
HinP1I GCGC 3 cut(s) 60, 117, 155
HinfI GANTC 2 cut(s) 395, 466
HpaII CCGG 5 cut(s) 37, 54, 63, 131, 164
Hpy188I TCNGA 2 cut(s) 241, 462
Hpy188III TCNNGA 1 cut(s) 223
Hpy99I CGWCG 2 cut(s) 176, 202
HpyAV CCTTC 4 cut(s) 93, 187, 331, 442
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 2 cut(s) 9, 315
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 1 cut(s) 266
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 2 cut(s) 13, 31
HspAI GCGC 3 cut(s) 60, 117, 155
KasI GGCGCC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 249, 340
LmnI GCTCC 2 cut(s) 225, 228
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 266
MalI GATC 2 cut(s) 251, 342
MboI GATC 2 cut(s) 249, 340
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 4 cut(s) 67, 85, 146, 439
Mly113I GGCGCC 1 cut(s) 60
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 3 cut(s) 219, 284, 456
MroNI GCCGGC 1 cut(s) 163
MspI CCGG 5 cut(s) 37, 54, 63, 131, 164
NaeI GCCGGC 1 cut(s) 165
NarI GGCGCC 1 cut(s) 60
NdeII GATC 2 cut(s) 249, 340
NgoMIV GCCGGC 1 cut(s) 163
NlaIII CATG 2 cut(s) 13, 31
NlaIV GGNNCC 1 cut(s) 61
NspI RCATGY 1 cut(s) 13
PdiI GCCGGC 1 cut(s) 165
PfeI GAWTC 2 cut(s) 395, 466
PkrI GCNGC 1 cut(s) 59
PluTI GGCGCC 1 cut(s) 63
Ppu21I YACGTR 1 cut(s) 267
Psp124BI GAGCTC 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 61
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SacI GAGCTC 1 cut(s) 144
SatI GCNGC 1 cut(s) 58
Sau3AI GATC 2 cut(s) 249, 340
SduI GDGCHC 1 cut(s) 144
SetI ASST 9 cut(s) 144, 222, 258, 269, 295, 305, 394, 412, 453
SfoI GGCGCC 1 cut(s) 61
Sse9I AATT 4 cut(s) 67, 85, 146, 439
SsiI CCGC 2 cut(s) 57, 185
SspDI GGCGCC 1 cut(s) 59
SspI AATATT 1 cut(s) 377
SspMI CTAG 1 cut(s) 407
SstI GAGCTC 1 cut(s) 144
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 1 cut(s) 269
TaqI TCGA 3 cut(s) 138, 144, 252
TaqII GACCGA 1 cut(s) 97
TasI AATT 4 cut(s) 67, 85, 146, 439
TatI WGTACW 1 cut(s) 12
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 2 cut(s) 395, 466
TscAI CASTG 1 cut(s) 479
TspDTI ATGAA 3 cut(s) 139, 296, 366
TspRI CASTG 1 cut(s) 479
XapI RAATTY 2 cut(s) 146, 439
XceI RCATGY 1 cut(s) 13
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.