Rh6AG517100

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011017
Physical Location & Seq
Reverse (-)
68656805 .. 68659186
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG517100.1

Sequence Viewer

Length: 474 bp
ATGTACTTCGCTAACGCCATGTCGTTCCGGCCAGTCCGATTCCCCGGCGCCGGAAATTACCAGAAGCCAACCAATTTTCTCGCCAGAAGGAAGTCGGTCTCCTGCGCGGGAAGTTCTCCGGAAATCGAGCTCGAATTCATAGCGCCGAAGCCGGCGAACGACGGGTCGTATGCGGTGGAGAAGGCGACGGCGATTAGTGGAGAGAAGCTCCTGAGGAGCATAATGTCGGATAACAAGATCGAGCTTTACGCCACATATGGGAAACTGATGAACTGTGGAGGTGGAGGAAGCTGTGGGACTTGCATTGTAGAGATTATTGATGGGAAGGATCTTTTGAATGAAAGAACGAATACTGAACTCAAATATTTGAAAAAGAAACCTGAATCTTGGAGACTAGCTTGCCAAACTATAGTGGGAAATAAAGAAAATTCTGGGAAGGTTCTTGTTCAGAGGATTCCCCAGTGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

157

Amino Acids

17.34

Weight (kDa)

9.4

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 90 - 138 9.4e-08 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32590 AT4G32590 AT4G32590 AT4G32590 AT4G32590
fragaria_vesca FvH4_2g30090 FvH4_2g30090
malus_domestica MD15G1116000.v1.1
prunus_persica Prupe.1G467300_v2.0.a1
pyrus_communis pycom15g10460
rosa_chinensis RchiOBHm_Chr6g0312831
rosa_laevigata RLG00000010294
rosa_multiflora Rmu_co8502815.1_g000001
rosa_roxburghii Rroxscaffold_7G00156450
rosa_rugosa Rorug06G0399900
rosa_samantha Rh6AG517100 Rh6BG528500 Rh6CG533200 Rh6DG519700
rosa_wichuraiana Rw6G044920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccII CGCG 1 cut(s) 107
AccIII TCCGGA 1 cut(s) 118
AciI CCGC 2 cut(s) 107, 173
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 29
AcsI RAATTY 2 cut(s) 134, 427
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 50, 258
AgsI TTSAA 2 cut(s) 337, 370
AluBI AGCT 5 cut(s) 130, 208, 244, 291, 398
AluI AGCT 5 cut(s) 130, 208, 244, 291, 398
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 2 cut(s) 103, 385
AlwI GGATC 1 cut(s) 336
Aor13HI TCCGGA 1 cut(s) 118
AoxI GGCC 1 cut(s) 29
ApoI RAATTY 2 cut(s) 134, 427
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 3 cut(s) 50, 107, 145
AsuC2I CCSGG 1 cut(s) 45
AxyI CCTNAGG 1 cut(s) 212
BanI GGYRCC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 132
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 204
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 2 cut(s) 103, 385
BfaI CTAG 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 408
BfoI RGCGCY 2 cut(s) 51, 146
Bme1390I CCNGG 1 cut(s) 45
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 45
BmrI ACTGGG 1 cut(s) 454
BmuI ACTGGG 1 cut(s) 454
BplI GAGNNNNNCTC 2 cut(s) 192, 224
BpuMI CCSGG 1 cut(s) 45
BsaHI GRCGYC 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 258
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 2 cut(s) 32, 460
Bse21I CCTNAGG 1 cut(s) 212
BseAI TCCGGA 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 50, 258
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 2 cut(s) 32, 460
BseRI GAGGAG 1 cut(s) 229
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 31
BshNI GGYRCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 5 cut(s) 28, 45, 51, 119, 152
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 2 cut(s) 50, 258
BsmAI GTCTC 2 cut(s) 103, 385
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 1 cut(s) 31
Bso31I GGTCTC 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 132
Bsp13I TCCGGA 1 cut(s) 118
Bsp143I GATC 2 cut(s) 237, 328
BspACI CCGC 2 cut(s) 107, 173
BspANI GGCC 1 cut(s) 31
BspCNI CTCAG 1 cut(s) 204
BspEI TCCGGA 1 cut(s) 118
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 1 cut(s) 49
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 47
BspTNI GGTCTC 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 151
BsrI ACTGG 2 cut(s) 32, 460
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 2 cut(s) 237, 328
BssNI GRCGYC 1 cut(s) 48
Bst4CI ACNGT 1 cut(s) 275
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 2 cut(s) 153, 400
BstDEI CTNAG 1 cut(s) 212
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 2 cut(s) 51, 146
BstHHI GCGC 3 cut(s) 50, 107, 145
BstKTI GATC 2 cut(s) 240, 331
BstMAI GTCTC 2 cut(s) 103, 385
BstMBI GATC 2 cut(s) 237, 328
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 408
BstUI CGCG 1 cut(s) 107
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
Bsu36I CCTNAGG 1 cut(s) 212
BsuRI GGCC 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 467
Cac8I GCNNGC 2 cut(s) 153, 400
CfoI GCGC 3 cut(s) 50, 107, 145
Cfr10I RCCGGY 1 cut(s) 151
Csp6I GTAC 1 cut(s) 4
CviAII CATG 1 cut(s) 19
CviJI RGCY 8 cut(s) 31, 67, 130, 151, 208, 244, 291, 398
CviKI_1 RGCY 8 cut(s) 31, 67, 130, 151, 208, 244, 291, 398
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 212
DinI GGCGCC 1 cut(s) 49
DpnI GATC 2 cut(s) 239, 330
DpnII GATC 2 cut(s) 237, 328
EaeI YGGCCR 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 130
Eco81I CCTNAGG 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 132
EgeI GGCGCC 1 cut(s) 49
EheI GGCGCC 1 cut(s) 49
FaeI CATG 1 cut(s) 22
FaiI YATR 7 cut(s) 20, 140, 171, 221, 256, 258, 410
FaqI GGGAC 1 cut(s) 310
FatI CATG 1 cut(s) 18
FauI CCCGC 1 cut(s) 100
FauNDI CATATG 1 cut(s) 256
FriOI GRGCYC 1 cut(s) 132
FspBI CTAG 1 cut(s) 395
GlaI GCGC 3 cut(s) 49, 106, 144
HaeII RGCGCY 2 cut(s) 51, 146
HaeIII GGCC 1 cut(s) 31
HapII CCGG 5 cut(s) 28, 45, 51, 119, 152
HhaI GCGC 3 cut(s) 50, 107, 145
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 3 cut(s) 48, 105, 143
HinP1I GCGC 3 cut(s) 48, 105, 143
HinfI GANTC 3 cut(s) 39, 383, 454
HpaII CCGG 5 cut(s) 28, 45, 51, 119, 152
Hpy188I TCNGA 3 cut(s) 38, 229, 450
Hpy188III TCNNGA 2 cut(s) 119, 211
Hpy99I CGWCG 2 cut(s) 164, 190
HpyAV CCTTC 4 cut(s) 81, 175, 319, 430
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4V TGCA 1 cut(s) 303
HpyF3I CTNAG 1 cut(s) 212
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 3 cut(s) 48, 105, 143
KasI GGCGCC 1 cut(s) 47
Kpn2I TCCGGA 1 cut(s) 118
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
Kzo9I GATC 2 cut(s) 237, 328
LmnI GCTCC 2 cut(s) 213, 216
MaeI CTAG 1 cut(s) 395
MalI GATC 2 cut(s) 239, 330
MboI GATC 2 cut(s) 237, 328
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 4 cut(s) 55, 73, 134, 427
Mly113I GGCGCC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 4 cut(s) 207, 272, 278, 444
MroI TCCGGA 1 cut(s) 118
MroNI GCCGGC 1 cut(s) 151
MspI CCGG 5 cut(s) 28, 45, 51, 119, 152
MspR9I CCNGG 1 cut(s) 45
MvnI CGCG 1 cut(s) 107
NaeI GCCGGC 1 cut(s) 153
NarI GGCGCC 1 cut(s) 48
NciI CCSGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 256
NdeII GATC 2 cut(s) 237, 328
NgoMIV GCCGGC 1 cut(s) 151
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 49
PdiI GCCGGC 1 cut(s) 153
PfeI GAWTC 3 cut(s) 39, 383, 454
PluTI GGCGCC 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 49
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SacI GAGCTC 1 cut(s) 132
Sau3AI GATC 2 cut(s) 237, 328
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 1 cut(s) 132
SetI ASST 8 cut(s) 132, 210, 246, 283, 293, 382, 400, 441
SfcI CTRYAG 1 cut(s) 408
SfoI GGCGCC 1 cut(s) 49
Sse9I AATT 4 cut(s) 55, 73, 134, 427
SsiI CCGC 2 cut(s) 107, 173
SspDI GGCGCC 1 cut(s) 47
SspI AATATT 1 cut(s) 365
SspMI CTAG 1 cut(s) 395
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 43
TaaI ACNGT 1 cut(s) 275
TaqI TCGA 3 cut(s) 126, 132, 240
TaqII GACCGA 1 cut(s) 85
TasI AATT 4 cut(s) 55, 73, 134, 427
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 3 cut(s) 39, 383, 454
TscAI CASTG 1 cut(s) 467
TspDTI ATGAA 3 cut(s) 127, 284, 354
TspRI CASTG 1 cut(s) 467
XapI RAATTY 2 cut(s) 134, 427
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.