MD15G1116000.v1.1

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011017
Physical Location & Seq
Reverse (-)
8167263 .. 8169783
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1116000.v1.1.491

Sequence Viewer

Length: 462 bp
ATGATTTCCCTGCACTTCGCAAACGCCGCGTCTTTCCGGCCAGCGCAAGTCTCCGGCGCCGGCCCCCGAAATTACCAACAGCCCACCGAGTTTTTCCCTAGAAGGAAGTGCGTTTCGGTCTGTTTCGCCGGTATTTCGGCGGATTCGTCTCCGACTCAGCCCGGAAAGCCCGAAATCGAGCTCGAATTCATCGGGCCGAAGCCGGGAAACGACGGGTCGTATCCGGTGGAGAGCGCGAAGGCGATAAGTGGAGAGAAGCTGCTGAGGGATGTCATGTCGGATAACAAGGTCGAGCTCTACGCCACGTATGGGAAACTGATGAACTGTGGAGGTGGTGGAAGCTGTGGGACTTGTATAGTTGAGAAACCGGAATCTTGGAGACTTGCTTGTCAAACTATAGTGGGAAATAAGGAAAATTCTGGCAAGGTTGTTGTTCAGAGGATTCCCCAGTGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

154

Amino Acids

16.53

Weight (kDa)

8.93

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32590 AT4G32590 AT4G32590 AT4G32590 AT4G32590
fragaria_vesca FvH4_2g30090 FvH4_2g30090
malus_domestica MD15G1116000.v1.1
prunus_persica Prupe.1G467300_v2.0.a1
pyrus_communis pycom15g10460
rosa_chinensis RchiOBHm_Chr6g0312831
rosa_laevigata RLG00000010294
rosa_multiflora Rmu_co8502815.1_g000001
rosa_roxburghii Rroxscaffold_7G00156450
rosa_rugosa Rorug06G0399900
rosa_samantha Rh6AG517100 Rh6BG528500 Rh6CG533200 Rh6DG519700
rosa_wichuraiana Rw6G044920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 387
AccB1I GGYRCC 1 cut(s) 56
AccII CGCG 2 cut(s) 29, 236
AciI CCGC 2 cut(s) 27, 140
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 2 cut(s) 185, 415
AcyI GRCGYC 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 203, 309
AluBI AGCT 4 cut(s) 181, 259, 295, 342
AluI AGCT 4 cut(s) 181, 259, 295, 342
Alw21I GWGCWC 2 cut(s) 183, 297
Alw26I GTCTC 3 cut(s) 55, 153, 373
AoxI GGCC 3 cut(s) 38, 61, 194
ApeKI GCWGC 1 cut(s) 259
ApoI RAATTY 2 cut(s) 185, 415
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AspLEI GCGC 3 cut(s) 46, 59, 236
AspS9I GGNCC 2 cut(s) 62, 194
AsuC2I CCSGG 2 cut(s) 162, 204
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 2 cut(s) 183, 297
Bbv12I GWGCWC 2 cut(s) 183, 297
BbvCI CCTCAGC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 246
BciVI GTATCC 1 cut(s) 231
BcnI CCSGG 2 cut(s) 162, 204
BcoDI GTCTC 3 cut(s) 55, 153, 373
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 396
BfoI RGCGCY 1 cut(s) 60
BfuI GTATCC 1 cut(s) 231
BisI GCNGC 2 cut(s) 27, 260
BlsI GCNGC 2 cut(s) 28, 261
Bme1390I CCNGG 2 cut(s) 162, 204
BmgT120I GGNCC 2 cut(s) 62, 194
BmiI GGNNCC 2 cut(s) 58, 64
BmrFI CCNGG 2 cut(s) 162, 204
BmrI ACTGGG 1 cut(s) 442
BmuI ACTGGG 1 cut(s) 442
Bpu10I CCTNAGC 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 162, 204
BsaAI YACGTR 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 57
BsaWI WCCGGW 2 cut(s) 223, 367
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 309
Bse118I RCCGGY 2 cut(s) 59, 128
Bse1I ACTGG 1 cut(s) 448
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 2 cut(s) 203, 309
BseMII CTCAG 2 cut(s) 170, 254
BseNI ACTGG 1 cut(s) 448
BseXI GCAGC 1 cut(s) 246
Bsh1236I CGCG 2 cut(s) 29, 236
BshFI GGCC 3 cut(s) 40, 63, 196
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 2 cut(s) 183, 297
BsiSI CCGG 8 cut(s) 37, 54, 60, 129, 162, 203, 224, 368
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 2 cut(s) 203, 309
BsmAI GTCTC 3 cut(s) 55, 153, 373
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 3 cut(s) 40, 63, 196
Bsp1286I GDGCHC 2 cut(s) 183, 297
BspACI CCGC 2 cut(s) 27, 140
BspANI GGCC 3 cut(s) 40, 63, 196
BspCNI CTCAG 2 cut(s) 169, 255
BspFNI CGCG 2 cut(s) 29, 236
BspLI GGNNCC 2 cut(s) 58, 64
BspT107I GGYRCC 1 cut(s) 56
BsrFI RCCGGY 2 cut(s) 59, 128
BsrI ACTGG 1 cut(s) 448
BssAI RCCGGY 2 cut(s) 59, 128
BssNI GRCGYC 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 326
BstACI GRCGYC 1 cut(s) 57
BstBAI YACGTR 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 42, 61
BstDEI CTNAG 2 cut(s) 156, 263
BstF5I GGATG 1 cut(s) 274
BstFNI CGCG 2 cut(s) 29, 236
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 3 cut(s) 46, 59, 236
BstMAI GTCTC 3 cut(s) 55, 153, 373
BstMWI GCNNNNNNNGC 2 cut(s) 26, 166
BstSCI CCNGG 2 cut(s) 160, 202
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 2 cut(s) 29, 236
BstV1I GCAGC 1 cut(s) 246
BsuI GTATCC 1 cut(s) 231
BsuRI GGCC 3 cut(s) 40, 63, 196
BtsCI GGATG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 455
Cac8I GCNNGC 2 cut(s) 42, 61
CfoI GCGC 3 cut(s) 46, 59, 236
Cfr10I RCCGGY 2 cut(s) 59, 128
Cfr13I GGNCC 2 cut(s) 62, 194
CseI GACGC 1 cut(s) 18
CviAII CATG 1 cut(s) 274
DdeI CTNAG 2 cut(s) 156, 263
DinI GGCGCC 1 cut(s) 58
DrdI GACNNNNNNGTC 1 cut(s) 387
DseDI GACNNNNNNGTC 1 cut(s) 387
EaeI YGGCCR 1 cut(s) 38
EciI GGCGGA 1 cut(s) 155
Ecl136II GAGCTC 2 cut(s) 181, 295
Eco24I GRGCYC 2 cut(s) 183, 297
Eco53kI GAGCTC 2 cut(s) 181, 295
EcoICRI GAGCTC 2 cut(s) 181, 295
EcoRI GAATTC 1 cut(s) 185
EcoT38I GRGCYC 2 cut(s) 183, 297
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 1 cut(s) 277
FaiI YATR 4 cut(s) 275, 309, 356, 398
FaqI GGGAC 1 cut(s) 361
FatI CATG 1 cut(s) 273
Fnu4HI GCNGC 2 cut(s) 27, 260
FokI GGATG 1 cut(s) 281
FriOI GRGCYC 2 cut(s) 183, 297
Fsp4HI GCNGC 2 cut(s) 27, 260
FspBI CTAG 1 cut(s) 99
GlaI GCGC 3 cut(s) 45, 58, 235
GluI GCNGC 2 cut(s) 27, 260
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 40, 63, 196
HapII CCGG 8 cut(s) 37, 54, 60, 129, 162, 203, 224, 368
HgaI GACGC 1 cut(s) 18
HhaI GCGC 3 cut(s) 46, 59, 236
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 3 cut(s) 44, 57, 234
HinP1I GCGC 3 cut(s) 44, 57, 234
HinfI GANTC 4 cut(s) 143, 154, 371, 442
HpaII CCGG 8 cut(s) 37, 54, 60, 129, 162, 203, 224, 368
Hpy188I TCNGA 3 cut(s) 153, 280, 438
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 96, 232
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 305
HpyCH4V TGCA 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 166
HpyF3I CTNAG 2 cut(s) 156, 263
HpySE526I ACGT 1 cut(s) 305
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 3 cut(s) 44, 57, 234
KasI GGCGCC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Lsp1109I GCAGC 1 cut(s) 246
MaeI CTAG 1 cut(s) 99
MaeII ACGT 1 cut(s) 305
MhlI GDGCHC 2 cut(s) 183, 297
MluCI AATT 3 cut(s) 70, 185, 415
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 1 cut(s) 148
MmeI TCCRAC 2 cut(s) 176, 258
MnlI CCTC 3 cut(s) 258, 323, 432
MroNI GCCGGC 1 cut(s) 59
MspI CCGG 8 cut(s) 37, 54, 60, 129, 162, 203, 224, 368
MspR9I CCNGG 2 cut(s) 162, 204
MvnI CGCG 2 cut(s) 29, 236
MwoI GCNNNNNNNGC 2 cut(s) 26, 166
NaeI GCCGGC 1 cut(s) 61
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 2 cut(s) 162, 204
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 1 cut(s) 277
NlaIV GGNNCC 2 cut(s) 58, 64
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PdiI GCCGGC 1 cut(s) 61
PfeI GAWTC 3 cut(s) 143, 371, 442
PkrI GCNGC 2 cut(s) 28, 261
PleI GAGTC 1 cut(s) 148
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 148
Ppu21I YACGTR 1 cut(s) 306
Psp124BI GAGCTC 2 cut(s) 183, 297
PspN4I GGNNCC 2 cut(s) 58, 64
PspPI GGNCC 2 cut(s) 62, 194
SacI GAGCTC 2 cut(s) 183, 297
SatI GCNGC 2 cut(s) 27, 260
Sau96I GGNCC 2 cut(s) 62, 194
SchI GAGTC 1 cut(s) 148
ScrFI CCNGG 2 cut(s) 162, 204
SduI GDGCHC 2 cut(s) 183, 297
SetI ASST 8 cut(s) 183, 261, 291, 297, 308, 334, 344, 429
SfcI CTRYAG 1 cut(s) 396
SfoI GGCGCC 1 cut(s) 58
Sse9I AATT 3 cut(s) 70, 185, 415
SsiI CCGC 2 cut(s) 27, 140
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 1 cut(s) 99
SstI GAGCTC 2 cut(s) 183, 297
StyD4I CCNGG 2 cut(s) 160, 202
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 308
TaqI TCGA 3 cut(s) 177, 183, 291
TaqII GACCGA 1 cut(s) 106
TasI AATT 3 cut(s) 70, 185, 415
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 3 cut(s) 143, 371, 442
TscAI CASTG 1 cut(s) 455
TseI GCWGC 1 cut(s) 259
TspDTI ATGAA 2 cut(s) 178, 335
TspRI CASTG 1 cut(s) 455
XapI RAATTY 2 cut(s) 185, 415
XspI CTAG 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.