Rroxscaffold_7G00156450

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011017
Physical Location & Seq
Forward (+)
293384 .. 295805
2422 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00156450.1

Sequence Viewer

Length: 483 bp
ATGGCTTGCATGTGCTTCGCTAACGCCATGTCGTTCCGGCCAGTCCGATTCTCCGGCACCGGAAATTACCAGAAGCCAACCAATTTTCTTGCCAGAAGGAAGTCGGTCTCCTGCGCGGGAAGTTCGCCAGAAATCGAGCTCGAATTCCTAGCGCCGAAGCCGGCAAACGACGGGTCGTATGCGGTGGAGAAGGCGACGGCGATTAGTGGGGAGAAGCTCCTAAGGAGCATAATGTCGGATAACAAGATCGAGCTTTACGCCACGTATGGGAAACTAATGAACTGTGGAGGTGTAGGAAGCTGTGGGACTTGCATTGTAGAGATTATTGATGGGAAGGATCTTTTGAATGAAAGAACGAATACTGAACTCAAATATTTAAAAAAGAAACCTGAATCTTGGAGACTAGCTTGCCAAACTATAGTGGGAAACAAAGAAAATTTTGGGAAGGTTCTTGTTCAGAGGATTCCCCAGTGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

160

Amino Acids

17.71

Weight (kDa)

9.28

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 93 - 141 1.3e-06 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32590 AT4G32590 AT4G32590 AT4G32590 AT4G32590
fragaria_vesca FvH4_2g30090 FvH4_2g30090
malus_domestica MD15G1116000.v1.1
prunus_persica Prupe.1G467300_v2.0.a1
pyrus_communis pycom15g10460
rosa_chinensis RchiOBHm_Chr6g0312831
rosa_laevigata RLG00000010294
rosa_multiflora Rmu_co8502815.1_g000001
rosa_roxburghii Rroxscaffold_7G00156450
rosa_rugosa Rorug06G0399900
rosa_samantha Rh6AG517100 Rh6BG528500 Rh6CG533200 Rh6DG519700
rosa_wichuraiana Rw6G044920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AccII CGCG 1 cut(s) 116
AciI CCGC 2 cut(s) 116, 182
AclWI GGATC 1 cut(s) 345
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 2 cut(s) 143, 436
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 1 cut(s) 346
AluBI AGCT 5 cut(s) 139, 217, 253, 300, 407
AluI AGCT 5 cut(s) 139, 217, 253, 300, 407
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 2 cut(s) 112, 394
AlwI GGATC 1 cut(s) 345
AoxI GGCC 1 cut(s) 38
ApoI RAATTY 2 cut(s) 143, 436
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AspLEI GCGC 2 cut(s) 116, 154
AxyI CCTNAGG 1 cut(s) 221
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 141
BccI CCATC 1 cut(s) 323
BceAI ACGGC 1 cut(s) 213
BcgI CGANNNNNNTGC 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 112, 394
BfaI CTAG 2 cut(s) 149, 404
BfmI CTRYAG 1 cut(s) 417
BfoI RGCGCY 1 cut(s) 155
BmiI GGNNCC 1 cut(s) 58
BmrI ACTGGG 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 463
BsaAI YACGTR 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 59
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 41, 469
Bse21I CCTNAGG 1 cut(s) 221
BseLI CCNNNNNNNGG 1 cut(s) 267
BseNI ACTGG 2 cut(s) 41, 469
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 1 cut(s) 40
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 4 cut(s) 37, 54, 60, 161
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 2 cut(s) 112, 394
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 1 cut(s) 40
Bso31I GGTCTC 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 2 cut(s) 246, 337
BspACI CCGC 2 cut(s) 116, 182
BspANI GGCC 1 cut(s) 40
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 58
BspPI GGATC 1 cut(s) 345
BspT107I GGYRCC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 2 cut(s) 41, 469
BssAI RCCGGY 1 cut(s) 160
BssMI GATC 2 cut(s) 246, 337
Bst4CI ACNGT 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 7, 162, 409
BstDEI CTNAG 1 cut(s) 221
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 2 cut(s) 116, 154
BstKTI GATC 2 cut(s) 249, 340
BstMAI GTCTC 2 cut(s) 112, 394
BstMBI GATC 2 cut(s) 246, 337
BstNSI RCATGY 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
Bsu36I CCTNAGG 1 cut(s) 221
BsuRI GGCC 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 476
Cac8I GCNNGC 3 cut(s) 7, 162, 409
CfoI GCGC 2 cut(s) 116, 154
Cfr10I RCCGGY 1 cut(s) 160
CviAII CATG 2 cut(s) 10, 28
CviJI RGCY 9 cut(s) 5, 40, 76, 139, 160, 217, 253, 300, 407
CviKI_1 RGCY 9 cut(s) 5, 40, 76, 139, 160, 217, 253, 300, 407
DdeI CTNAG 1 cut(s) 221
DpnI GATC 2 cut(s) 248, 339
DpnII GATC 2 cut(s) 246, 337
DraI TTTAAA 1 cut(s) 378
EaeI YGGCCR 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 139
Eco81I CCTNAGG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 141
FaeI CATG 2 cut(s) 13, 31
FaiI YATR 6 cut(s) 11, 29, 180, 230, 267, 419
FaqI GGGAC 1 cut(s) 319
FatI CATG 2 cut(s) 9, 27
FauI CCCGC 1 cut(s) 109
FriOI GRGCYC 1 cut(s) 141
FspBI CTAG 2 cut(s) 149, 404
GlaI GCGC 2 cut(s) 115, 153
HaeII RGCGCY 1 cut(s) 155
HaeIII GGCC 1 cut(s) 40
HapII CCGG 4 cut(s) 37, 54, 60, 161
HhaI GCGC 2 cut(s) 116, 154
Hin1II CATG 2 cut(s) 13, 31
Hin6I GCGC 2 cut(s) 114, 152
HinP1I GCGC 2 cut(s) 114, 152
HinfI GANTC 3 cut(s) 48, 392, 463
HpaII CCGG 4 cut(s) 37, 54, 60, 161
Hpy188I TCNGA 3 cut(s) 47, 238, 459
Hpy99I CGWCG 2 cut(s) 173, 199
HpyAV CCTTC 4 cut(s) 90, 184, 328, 439
HpyCH4III ACNGT 1 cut(s) 284
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 2 cut(s) 9, 312
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 1 cut(s) 263
Hsp92II CATG 2 cut(s) 13, 31
HspAI GCGC 2 cut(s) 114, 152
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
Kzo9I GATC 2 cut(s) 246, 337
LmnI GCTCC 2 cut(s) 222, 225
MaeI CTAG 2 cut(s) 149, 404
MaeII ACGT 1 cut(s) 263
MalI GATC 2 cut(s) 248, 339
MboI GATC 2 cut(s) 246, 337
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 4 cut(s) 64, 82, 143, 436
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 2 cut(s) 281, 453
MroNI GCCGGC 1 cut(s) 160
MseI TTAA 1 cut(s) 377
MspI CCGG 4 cut(s) 37, 54, 60, 161
MvnI CGCG 1 cut(s) 116
NaeI GCCGGC 1 cut(s) 162
NdeII GATC 2 cut(s) 246, 337
NgoMIV GCCGGC 1 cut(s) 160
NlaIII CATG 2 cut(s) 13, 31
NlaIV GGNNCC 1 cut(s) 58
NspI RCATGY 1 cut(s) 13
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 3 cut(s) 48, 392, 463
Ppu21I YACGTR 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 58
PsuI RGATCY 1 cut(s) 337
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 377
Sau3AI GATC 2 cut(s) 246, 337
SduI GDGCHC 1 cut(s) 141
SetI ASST 9 cut(s) 141, 219, 255, 266, 292, 302, 391, 409, 450
SfcI CTRYAG 1 cut(s) 417
Sse9I AATT 4 cut(s) 64, 82, 143, 436
SsiI CCGC 2 cut(s) 116, 182
SspI AATATT 1 cut(s) 374
SspMI CTAG 2 cut(s) 149, 404
SstI GAGCTC 1 cut(s) 141
TaaI ACNGT 1 cut(s) 284
TaiI ACGT 1 cut(s) 266
TaqI TCGA 3 cut(s) 135, 141, 249
TaqII GACCGA 1 cut(s) 94
TasI AATT 4 cut(s) 64, 82, 143, 436
TfiI GAWTC 3 cut(s) 48, 392, 463
Tru1I TTAA 1 cut(s) 377
Tru9I TTAA 1 cut(s) 377
TscAI CASTG 1 cut(s) 476
TspDTI ATGAA 2 cut(s) 293, 363
TspRI CASTG 1 cut(s) 476
XapI RAATTY 2 cut(s) 143, 436
XceI RCATGY 1 cut(s) 13
XspI CTAG 2 cut(s) 149, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.