Rw6G044920

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011017
Physical Location & Seq
Reverse (-)
68019441 .. 68021504
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G044920.1

Sequence Viewer

Length: 483 bp
ATGGCTTGCATGTACTTCGCTAACGCCATGTCGTTCCGGCCAGTCCGATTCTCCGGCGCCGGAAATTACCATACGCCAACCAATTTTCTTGCCAGAAGGAAGTCGGTCTGCTGCGCGGGAAGTTCGCCGGAAATCGAGCTCGAATTCATAGCGCCGAAGCCGGCGAACGATGGGTCGTATGCGGTGGAGAAGGCGACGGCGATTAGTGGAGAGAAGCTCCTGAGGAGCATAATGTCGGATAACAAGATCGAGCTTTACGCCACATATGGGAAACTGATGAACTGTGGAGGTGGAGGAAGCTGTGGGACTTGCATTGTAGAGATTATTGATGGGAATGATCTTTTGAATGAAAGAACGAATACTGAACTCAAATATTTGAAAAAGAAACCTGAATCTTGGAGACTAGCTTGCCAAACTATAGTGGGAAATAAAGAAAATTCTGGGAAGGTTCTTGTTCAGAAGATTCCCCAGTGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

160

Amino Acids

17.6

Weight (kDa)

9.07

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 93 - 141 1.5e-07 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32590 AT4G32590 AT4G32590 AT4G32590 AT4G32590
fragaria_vesca FvH4_2g30090 FvH4_2g30090
malus_domestica MD15G1116000.v1.1
prunus_persica Prupe.1G467300_v2.0.a1
pyrus_communis pycom15g10460
rosa_chinensis RchiOBHm_Chr6g0312831
rosa_laevigata RLG00000010294
rosa_multiflora Rmu_co8502815.1_g000001
rosa_roxburghii Rroxscaffold_7G00156450
rosa_rugosa Rorug06G0399900
rosa_samantha Rh6AG517100 Rh6BG528500 Rh6CG533200 Rh6DG519700
rosa_wichuraiana Rw6G044920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AccII CGCG 1 cut(s) 116
AciI CCGC 2 cut(s) 116, 182
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 2 cut(s) 143, 436
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 2 cut(s) 346, 379
AluBI AGCT 5 cut(s) 139, 217, 253, 300, 407
AluI AGCT 5 cut(s) 139, 217, 253, 300, 407
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 394
AoxI GGCC 1 cut(s) 38
ApeKI GCWGC 1 cut(s) 111
ApoI RAATTY 2 cut(s) 143, 436
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AspLEI GCGC 3 cut(s) 59, 116, 154
AxyI CCTNAGG 1 cut(s) 221
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 98
BccI CCATC 2 cut(s) 164, 323
BceAI ACGGC 1 cut(s) 213
BcgI CGANNNNNNTGC 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 394
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 417
BfoI RGCGCY 2 cut(s) 60, 155
BisI GCNGC 1 cut(s) 112
BlsI GCNGC 1 cut(s) 113
BmiI GGNNCC 1 cut(s) 58
BmrI ACTGGG 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 463
BplI GAGNNNNNCTC 2 cut(s) 201, 233
BsaHI GRCGYC 1 cut(s) 57
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 41, 469
Bse21I CCTNAGG 1 cut(s) 221
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 2 cut(s) 41, 469
BseRI GAGGAG 1 cut(s) 238
BseXI GCAGC 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 1 cut(s) 40
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 5 cut(s) 37, 54, 60, 128, 161
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 394
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 2 cut(s) 246, 337
BspACI CCGC 2 cut(s) 116, 182
BspANI GGCC 1 cut(s) 40
BspCNI CTCAG 1 cut(s) 213
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 2 cut(s) 41, 469
BssAI RCCGGY 1 cut(s) 160
BssMI GATC 2 cut(s) 246, 337
BssNI GRCGYC 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 284
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 7, 162, 409
BstDEI CTNAG 1 cut(s) 221
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 2 cut(s) 60, 155
BstHHI GCGC 3 cut(s) 59, 116, 154
BstKTI GATC 2 cut(s) 249, 340
BstMAI GTCTC 1 cut(s) 394
BstMBI GATC 2 cut(s) 246, 337
BstNSI RCATGY 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 98
Bsu36I CCTNAGG 1 cut(s) 221
BsuRI GGCC 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 476
Cac8I GCNNGC 3 cut(s) 7, 162, 409
CfoI GCGC 3 cut(s) 59, 116, 154
Cfr10I RCCGGY 1 cut(s) 160
Csp6I GTAC 1 cut(s) 13
CviAII CATG 2 cut(s) 10, 28
CviJI RGCY 8 cut(s) 5, 40, 139, 160, 217, 253, 300, 407
CviKI_1 RGCY 8 cut(s) 5, 40, 139, 160, 217, 253, 300, 407
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 1 cut(s) 221
DinI GGCGCC 1 cut(s) 58
DpnI GATC 2 cut(s) 248, 339
DpnII GATC 2 cut(s) 246, 337
EaeI YGGCCR 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 139
Eco81I CCTNAGG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 141
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
FaeI CATG 2 cut(s) 13, 31
FaiI YATR 9 cut(s) 11, 29, 72, 149, 180, 230, 265, 267, 419
FaqI GGGAC 1 cut(s) 319
FatI CATG 2 cut(s) 9, 27
FauI CCCGC 1 cut(s) 109
FauNDI CATATG 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 112
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 112
FspBI CTAG 1 cut(s) 404
GlaI GCGC 3 cut(s) 58, 115, 153
GluI GCNGC 1 cut(s) 112
HaeII RGCGCY 2 cut(s) 60, 155
HaeIII GGCC 1 cut(s) 40
HapII CCGG 5 cut(s) 37, 54, 60, 128, 161
HhaI GCGC 3 cut(s) 59, 116, 154
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 2 cut(s) 13, 31
Hin6I GCGC 3 cut(s) 57, 114, 152
HinP1I GCGC 3 cut(s) 57, 114, 152
HinfI GANTC 3 cut(s) 48, 392, 463
HpaII CCGG 5 cut(s) 37, 54, 60, 128, 161
Hpy188I TCNGA 3 cut(s) 47, 238, 459
Hpy188III TCNNGA 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 3 cut(s) 90, 184, 439
HpyCH4III ACNGT 1 cut(s) 284
HpyCH4V TGCA 2 cut(s) 9, 312
HpyF3I CTNAG 1 cut(s) 221
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 2 cut(s) 13, 31
HspAI GCGC 3 cut(s) 57, 114, 152
KasI GGCGCC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
Kzo9I GATC 2 cut(s) 246, 337
LmnI GCTCC 2 cut(s) 222, 225
Lsp1109I GCAGC 1 cut(s) 98
MaeI CTAG 1 cut(s) 404
MalI GATC 2 cut(s) 248, 339
MboI GATC 2 cut(s) 246, 337
MboII GAAGA 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 4 cut(s) 64, 82, 143, 436
Mly113I GGCGCC 1 cut(s) 57
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 3 cut(s) 216, 281, 287
MroNI GCCGGC 1 cut(s) 160
MspI CCGG 5 cut(s) 37, 54, 60, 128, 161
MvnI CGCG 1 cut(s) 116
NaeI GCCGGC 1 cut(s) 162
NarI GGCGCC 1 cut(s) 57
NdeI CATATG 1 cut(s) 265
NdeII GATC 2 cut(s) 246, 337
NgoMIV GCCGGC 1 cut(s) 160
NlaIII CATG 2 cut(s) 13, 31
NlaIV GGNNCC 1 cut(s) 58
NspI RCATGY 1 cut(s) 13
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 3 cut(s) 48, 392, 463
PkrI GCNGC 1 cut(s) 113
PluTI GGCGCC 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 58
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SacI GAGCTC 1 cut(s) 141
SatI GCNGC 1 cut(s) 112
Sau3AI GATC 2 cut(s) 246, 337
SduI GDGCHC 1 cut(s) 141
SetI ASST 8 cut(s) 141, 219, 255, 292, 302, 391, 409, 450
SfcI CTRYAG 1 cut(s) 417
SfoI GGCGCC 1 cut(s) 58
Sse9I AATT 4 cut(s) 64, 82, 143, 436
SsiI CCGC 2 cut(s) 116, 182
SspDI GGCGCC 1 cut(s) 56
SspI AATATT 1 cut(s) 374
SspMI CTAG 1 cut(s) 404
SstI GAGCTC 1 cut(s) 141
TaaI ACNGT 1 cut(s) 284
TaqI TCGA 3 cut(s) 135, 141, 249
TaqII GACCGA 1 cut(s) 94
TasI AATT 4 cut(s) 64, 82, 143, 436
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 3 cut(s) 48, 392, 463
TscAI CASTG 1 cut(s) 476
TseI GCWGC 1 cut(s) 111
TspDTI ATGAA 3 cut(s) 136, 293, 363
TspRI CASTG 1 cut(s) 476
XapI RAATTY 2 cut(s) 143, 436
XceI RCATGY 1 cut(s) 13
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.