AT4G33140

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012369
Physical Location & Seq
Forward (+)
15981391 .. 15984352
2962 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33140.1

Sequence Viewer

Length: 1062 bp
ATGTTAAAGAGGCACTCTATCGCCATTCTCTCCAGAATCGCAAGCTTCGATTCCCATAAAACCCTTATGTCTTCCTTTACCGATCCCCTTTTTCTGCGATCGCTTTACTCTCCTCGATCCATCGCCATCAGATTTCATGGAGATTTTGCTCATAGACTCTACAATCCCATTCAAATTTACAACAACAACAACAACAACAGTGGCTCTTGTAATGGTTTGTTCAAGGTTAAAGGTGGAGGAGGAAAGCCGAAAGGATTCACCTTGCGAAGTTGTTTTGATTCTCATAAGAAACCTGATCAAAAGGCTCGAGCTTTAACTCAACATCGCCGCTTTCTCCATGATCTGGGATCTGGGCCTAGTGAAGAACGCGCCGCTGCCGGAACTACCGACCTTGCCGATGGCCGTGGTGCATATCTGCGTACGAGGTTTCAAGGAAATGATAAGATTGTTGTGGCTGTTGACATAGATGAGGTTCTTGGAAACTTTGTCTCGGCCCTTAACAGATTCATTGCGGACCGCTATTTGTCGAACCATTCAGTCTCAGAATACCATGTCTATGAGTTCTTCAAGATATGGAACTGCTCTCGCAACGAAGCCGACTTACGTGTTCATGAATTCTTTAAGACATCATATTTCAAGAAAGGGATCCATCCTCTTCCAGGTGCCCATAAGACTCTTCACAAGCTATCAAAATATTGCGACATGTCAGTTGTGACGTCCCGGCAGAATGCGATAAAGGAGCACACACTTGAGTGGATCGAGATACATTTCCCGGGACTATTTAAACAGATTCATTTTGGGAACCATTTTGCTCTTCATGGAGAGTCTAGACCAAAGTCTGAAATCTGCAGATCATTTGGAGCAGAGATATTGATTGACGATAACCCAAGATATGCCGAGGAATGCGCTAACATTGGAATGAAGGTTCTCCTCTTTGACTATGAAAATTCATACCCTTGGTCTAAGACAGAGTCTGTGGATCGGCATCCTTTGGTGACTAGAGTTCATAATTGGGAAGAGGTGGAGCAGCAGATTCTCTCATTGGCAGTGTCAAAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

353

Amino Acids

40.39

Weight (kDa)

9.11

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT5C PF06941 149 - 346 4.6e-16 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33140
fragaria_vesca FvH4_1g09650 FvH4_1g09650
malus_domestica MD02G1105400.v1.1 MD15G1225300.v1.1
prunus_persica Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1
pyrus_communis pycom15g20010
rosa_chinensis RchiOBHm_Chr2g0096491
rosa_laevigata RLG00000016644
rosa_multiflora Rmu_sc0004096.1_g000012
rosa_roxburghii Rroxscaffold_2G00145600
rosa_rugosa Rorug02G0058100
rosa_samantha Rh2AG105100 Rh2BG107200 Rh2CG109000 Rh2DG108200
rosa_wichuraiana Rw2G008120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 662
AccB7I CCANNNNNTGG 1 cut(s) 343
AccII CGCG 1 cut(s) 369
AciI CCGC 4 cut(s) 328, 372, 512, 517
AclWI GGATC 7 cut(s) 77, 111, 355, 640, 653, 764, 987
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 3 cut(s) 174, 614, 946
AcyI GRCGYC 1 cut(s) 716
AfaI GTAC 1 cut(s) 421
AfiI CCNNNNNNNGG 2 cut(s) 343, 659
AflIII ACRYGT 2 cut(s) 604, 702
AgsI TTSAA 5 cut(s) 173, 223, 431, 568, 637
AjnI CCWGG 1 cut(s) 658
AleI CACNNNNGTG 1 cut(s) 751
AloI GAACNNNNNNTCC 2 cut(s) 909, 941
AluBI AGCT 3 cut(s) 45, 311, 685
AluI AGCT 3 cut(s) 45, 311, 685
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 2 cut(s) 493, 544
AlwI GGATC 7 cut(s) 77, 111, 355, 640, 653, 764, 987
AlwNI CAGNNNCTG 1 cut(s) 974
Ama87I CYCGRG 2 cut(s) 306, 772
AoxI GGCC 3 cut(s) 353, 400, 492
ApeKI GCWGC 2 cut(s) 374, 1027
ApoI RAATTY 3 cut(s) 174, 614, 946
AsiSI GCGATCGC 1 cut(s) 101
Asp700I GAANNNNTTC 1 cut(s) 254
AspLEI GCGC 2 cut(s) 371, 908
AspS9I GGNCC 3 cut(s) 353, 493, 514
AsuC2I CCSGG 3 cut(s) 721, 773, 774
AsuHPI GGTGA 2 cut(s) 250, 1006
AvaI CYCGRG 2 cut(s) 306, 772
AvaII GGWCC 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 667
BamHI GGATCC 1 cut(s) 645
BanI GGYRCC 1 cut(s) 662
BarI GAAGNNNNNNTAC 2 cut(s) 585, 617
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 744
BbvI GCAGC 2 cut(s) 361, 1039
BccI CCATC 4 cut(s) 128, 134, 392, 657
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 1 cut(s) 660
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 3 cut(s) 721, 773, 774
BcoDI GTCTC 2 cut(s) 493, 544
BfaI CTAG 3 cut(s) 357, 828, 999
BfmI CTRYAG 1 cut(s) 847
BisI GCNGC 4 cut(s) 328, 372, 375, 1028
BlsI GCNGC 4 cut(s) 329, 373, 376, 1029
Bme1390I CCNGG 4 cut(s) 660, 721, 773, 774
Bme18I GGWCC 1 cut(s) 514
BmeT110I CYCGRG 2 cut(s) 306, 772
BmgT120I GGNCC 3 cut(s) 353, 493, 514
BmiI GGNNCC 3 cut(s) 647, 664, 803
BmrFI CCNGG 4 cut(s) 660, 721, 773, 774
BmsI GCATC 1 cut(s) 994
BoxI GACNNNNGTC 1 cut(s) 835
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 770
BpuMI CCSGG 3 cut(s) 721, 773, 774
BsaAI YACGTR 1 cut(s) 605
BsaBI GATNNNNATC 1 cut(s) 984
BsaHI GRCGYC 1 cut(s) 716
BsaJI CCNNGG 4 cut(s) 403, 772, 897, 956
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 659
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 984
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 4 cut(s) 403, 772, 897, 956
BseGI GGATG 2 cut(s) 649, 985
BseJI GATNNNNATC 1 cut(s) 984
BseLI CCNNNNNNNGG 2 cut(s) 343, 659
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 1 cut(s) 555
BseRI GAGGAG 3 cut(s) 102, 252, 920
BseSI GKGCMC 1 cut(s) 667
BseXI GCAGC 2 cut(s) 361, 1039
Bsh1236I CGCG 1 cut(s) 369
Bsh1285I CGRYCG 1 cut(s) 101
BshFI GGCC 3 cut(s) 355, 402, 494
BshNI GGYRCC 1 cut(s) 662
BsiEI CGRYCG 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 744
BsiHKCI CYCGRG 2 cut(s) 306, 772
BsiSI CCGG 3 cut(s) 378, 721, 773
BsiWI CGTACG 1 cut(s) 419
BslFI GGGAC 2 cut(s) 703, 789
BslI CCNNNNNNNGG 2 cut(s) 343, 659
BsmAI GTCTC 2 cut(s) 493, 544
BsmFI GGGAC 2 cut(s) 703, 789
BsmI GAATGC 2 cut(s) 733, 908
BsnI GGCC 3 cut(s) 355, 402, 494
BsoBI CYCGRG 2 cut(s) 306, 772
Bsp1286I GDGCHC 2 cut(s) 667, 744
BspACI CCGC 4 cut(s) 328, 372, 512, 517
BspANI GGCC 3 cut(s) 355, 402, 494
BspCNI CTCAG 1 cut(s) 554
BspFNI CGCG 1 cut(s) 369
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 3 cut(s) 647, 664, 803
BspMAI CTGCAG 1 cut(s) 851
BspPI GGATC 7 cut(s) 77, 111, 355, 640, 653, 764, 987
BspQI GCTCTTC 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 507
BssECI CCNNGG 4 cut(s) 403, 772, 897, 956
BssNI GRCGYC 1 cut(s) 716
BssT1I CCWWGG 1 cut(s) 956
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 4 cut(s) 660, 681, 819, 1011
BstACI GRCGYC 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 605
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 2 cut(s) 541, 963
BstDSI CCRYGG 1 cut(s) 403
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 2 cut(s) 649, 985
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 2 cut(s) 371, 908
BstMAI GTCTC 2 cut(s) 493, 544
BstMCI CGRYCG 1 cut(s) 101
BstNI CCWGG 1 cut(s) 660
BstNSI RCATGY 1 cut(s) 706
BstPAI GACNNNNGTC 1 cut(s) 835
BstSCI CCNGG 4 cut(s) 658, 719, 771, 772
BstSFI CTRYAG 1 cut(s) 847
BstSLI GKGCMC 1 cut(s) 667
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 2 cut(s) 361, 1039
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 2 cut(s) 347, 645
BstYI RGATCY 2 cut(s) 347, 645
BsuRI GGCC 3 cut(s) 355, 402, 494
BtgI CCRYGG 1 cut(s) 403
BtgZI GCGATG 2 cut(s) 106, 308
BtsCI GGATG 2 cut(s) 649, 985
BtsI GCAGTG 1 cut(s) 1053
BtsIMutI CAGTG 2 cut(s) 205, 1053
Cac8I GCNNGC 1 cut(s) 43
CaiI CAGNNNCTG 1 cut(s) 974
CciI TCATGA 1 cut(s) 610
CfoI GCGC 2 cut(s) 371, 908
Cfr13I GGNCC 3 cut(s) 353, 493, 514
Cfr9I CCCGGG 1 cut(s) 772
CpoI CGGWCCG 1 cut(s) 514
Csp6I GTAC 1 cut(s) 420
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CspI CGGWCCG 1 cut(s) 514
CviAII CATG 6 cut(s) 137, 338, 551, 611, 703, 818
CviQI GTAC 1 cut(s) 420
DdeI CTNAG 2 cut(s) 541, 963
DraI TTTAAA 1 cut(s) 784
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 4 cut(s) 660, 681, 819, 1011
EarI CTCTTC 4 cut(s) 660, 681, 819, 1011
Eco130I CCWWGG 1 cut(s) 956
Eco47I GGWCC 1 cut(s) 514
Eco88I CYCGRG 2 cut(s) 306, 772
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoRI GAATTC 1 cut(s) 614
EcoRII CCWGG 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 956
ErhI CCWWGG 1 cut(s) 956
FaeI CATG 6 cut(s) 140, 341, 554, 614, 706, 821
FaqI GGGAC 2 cut(s) 703, 789
FatI CATG 6 cut(s) 136, 337, 550, 610, 702, 817
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 4 cut(s) 328, 372, 375, 1028
FokI GGATG 2 cut(s) 636, 972
Fsp4HI GCNGC 4 cut(s) 328, 372, 375, 1028
FspBI CTAG 3 cut(s) 357, 828, 999
GlaI GCGC 2 cut(s) 370, 907
GluI GCNGC 4 cut(s) 328, 372, 375, 1028
GsuI CTGGAG 1 cut(s) 16
HaeIII GGCC 3 cut(s) 355, 402, 494
HapII CCGG 3 cut(s) 378, 721, 773
HhaI GCGC 2 cut(s) 371, 908
Hin1I GRCGYC 1 cut(s) 716
Hin1II CATG 6 cut(s) 140, 341, 554, 614, 706, 821
Hin6I GCGC 2 cut(s) 369, 906
HinP1I GCGC 2 cut(s) 369, 906
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 43
HpaII CCGG 3 cut(s) 378, 721, 773
HphI GGTGA 2 cut(s) 250, 1006
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 3 cut(s) 131, 544, 841
Hpy188III TCNNGA 6 cut(s) 33, 568, 611, 637, 760, 828
Hpy8I GTNNAC 1 cut(s) 460
HpyAV CCTTC 1 cut(s) 916
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 2 cut(s) 604, 716
HpyCH4V TGCA 2 cut(s) 410, 849
HpyF3I CTNAG 2 cut(s) 541, 963
HpySE526I ACGT 2 cut(s) 604, 716
Hsp92I GRCGYC 1 cut(s) 716
Hsp92II CATG 6 cut(s) 140, 341, 554, 614, 706, 821
HspAI GCGC 2 cut(s) 369, 906
Ksp22I TGATCA 1 cut(s) 295
LguI GCTCTTC 1 cut(s) 819
LmnI GCTCC 3 cut(s) 739, 860, 1024
LpnPI CCDG 9 cut(s) 46, 306, 329, 336, 391, 645, 672, 734, 786
Lsp1109I GCAGC 2 cut(s) 361, 1039
LweI GCATC 1 cut(s) 994
MaeI CTAG 3 cut(s) 357, 828, 999
MaeII ACGT 2 cut(s) 604, 716
MaeIII GTNAC 2 cut(s) 712, 994
MboII GAAGA 7 cut(s) 63, 374, 556, 647, 668, 806, 1028
MflI RGATCY 2 cut(s) 347, 645
MhlI GDGCHC 2 cut(s) 667, 744
MluCI AATT 4 cut(s) 174, 614, 946, 1009
MlyI GAGTC 4 cut(s) 150, 667, 833, 980
MroXI GAANNNNTTC 1 cut(s) 254
MseI TTAA 7 cut(s) 5, 228, 314, 498, 621, 783, 1060
MslI CAYNNNNRTG 3 cut(s) 555, 751, 917
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 3 cut(s) 378, 721, 773
MspR9I CCNGG 4 cut(s) 660, 721, 773, 774
Mva1269I GAATGC 2 cut(s) 733, 908
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 1 cut(s) 369
NciI CCSGG 3 cut(s) 721, 773, 774
NlaIII CATG 6 cut(s) 140, 341, 554, 614, 706, 821
NlaIV GGNNCC 3 cut(s) 647, 664, 803
NmeAIII GCCGAG 2 cut(s) 470, 922
NmuCI GTSAC 2 cut(s) 712, 994
NspI RCATGY 1 cut(s) 706
OliI CACNNNNGTG 1 cut(s) 751
PaeR7I CTCGAG 1 cut(s) 306
PagI TCATGA 1 cut(s) 610
PciI ACATGT 1 cut(s) 702
PciSI GCTCTTC 1 cut(s) 819
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 2 cut(s) 733, 908
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 7 cut(s) 36, 50, 255, 278, 504, 790, 1033
Pfl23II CGTACG 1 cut(s) 419
PflFI GACNNNGTC 1 cut(s) 970
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 4 cut(s) 329, 373, 376, 1029
Ple19I CGATCG 1 cut(s) 101
PleI GAGTC 4 cut(s) 150, 667, 832, 979
PpsI GAGTC 4 cut(s) 150, 667, 832, 979
Ppu21I YACGTR 1 cut(s) 605
PscI ACATGT 1 cut(s) 702
PshAI GACNNNNGTC 1 cut(s) 835
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspLI CGTACG 1 cut(s) 419
PspN4I GGNNCC 3 cut(s) 647, 664, 803
PspPI GGNCC 3 cut(s) 353, 493, 514
PspXI VCTCGAGB 1 cut(s) 306
PstI CTGCAG 1 cut(s) 851
PstNI CAGNNNCTG 1 cut(s) 974
PsuI RGATCY 2 cut(s) 347, 645
PsyI GACNNNGTC 1 cut(s) 970
PvuI CGATCG 1 cut(s) 101
RgaI GCGATCGC 1 cut(s) 101
RsaI GTAC 1 cut(s) 421
RsaNI GTAC 1 cut(s) 420
RseI CAYNNNNRTG 3 cut(s) 555, 751, 917
Rsr2I CGGWCCG 1 cut(s) 514
RsrII CGGWCCG 1 cut(s) 514
SapI GCTCTTC 1 cut(s) 819
SaqAI TTAA 7 cut(s) 5, 228, 314, 498, 621, 783, 1060
SatI GCNGC 4 cut(s) 328, 372, 375, 1028
Sau96I GGNCC 3 cut(s) 353, 493, 514
SchI GAGTC 4 cut(s) 150, 667, 833, 980
ScrFI CCNGG 4 cut(s) 660, 721, 773, 774
SduI GDGCHC 2 cut(s) 667, 744
SfaAI GCGATCGC 1 cut(s) 101
SfaNI GCATC 1 cut(s) 994
SfcI CTRYAG 1 cut(s) 847
Sfr274I CTCGAG 1 cut(s) 306
SgfI GCGATCGC 1 cut(s) 101
SinI GGWCC 1 cut(s) 514
SlaI CTCGAG 1 cut(s) 306
SmaI CCCGGG 1 cut(s) 774
SmiMI CAYNNNNRTG 3 cut(s) 555, 751, 917
SmlI CTYRAG 2 cut(s) 306, 749
SmoI CTYRAG 2 cut(s) 306, 749
Sse9I AATT 4 cut(s) 174, 614, 946, 1009
SsiI CCGC 4 cut(s) 328, 372, 512, 517
SspI AATATT 1 cut(s) 695
SspMI CTAG 3 cut(s) 357, 828, 999
StyD4I CCNGG 4 cut(s) 658, 719, 771, 772
StyI CCWWGG 1 cut(s) 956
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 2 cut(s) 607, 719
TaqI TCGA 5 cut(s) 48, 115, 307, 527, 759
TasI AATT 4 cut(s) 174, 614, 946, 1009
TauI GCSGC 2 cut(s) 330, 374
TfiI GAWTC 7 cut(s) 36, 50, 255, 278, 504, 790, 1033
Tru1I TTAA 7 cut(s) 5, 228, 314, 498, 621, 783, 1060
Tru9I TTAA 7 cut(s) 5, 228, 314, 498, 621, 783, 1060
TscAI CASTG 2 cut(s) 205, 1053
TseFI GTSAC 2 cut(s) 712, 994
TseI GCWGC 2 cut(s) 374, 1027
Tsp45I GTSAC 2 cut(s) 712, 994
TspMI CCCGGG 1 cut(s) 772
TspRI CASTG 2 cut(s) 205, 1053
Tth111I GACNNNGTC 1 cut(s) 970
Van91I CCANNNNNTGG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 514
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 3 cut(s) 174, 614, 946
XbaI TCTAGA 1 cut(s) 827
XceI RCATGY 1 cut(s) 706
XhoI CTCGAG 1 cut(s) 306
XmaI CCCGGG 1 cut(s) 772
XmnI GAANNNNTTC 1 cut(s) 254
XspI CTAG 3 cut(s) 357, 828, 999
ZraI GACGTC 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.