MD15G1225300.v1.1

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012369
Physical Location & Seq
Reverse (-)
18308326 .. 18311525
3200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1225300.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGTTGTTGTTGAGAAGGCAAGGCGTCGATCGCTTCCTCTTCGCAAGCTCAAAGCACCGACCTTTGCTCCTCCATCGCTGCCAGCACTTTACAGGGGCCATGGCGGCTGCTTTTATGGGTAGCCATAGTGGATGCAGTGGGTTTCATTCCCAAGCTCAATGGCCGCCGTCCCATTTTTCCGCGGACCATTCTTGCAAGTTTGCCAATTTTCCCGGGAAAGCTGGTACTTTACCTGGGAATCTGAGGAATACAAGCATTGGGTGCAATTTTGCTGTGAAGGGGTGCTTGCAATCTCAGCATCATCCCCACCATCAGTCTCATGGCAGCATTAAGCGTCATCCCAATGCTCAGTTGGTTCGTGATCATGAACTGGAGCTCCGCAGCCCGGATGCTGTTGGATTGGCCTCCTCAAATGGTTGCCAGGAGCACGGAGGCCGGTCCAGAATCCCACACCATCCTCTGCCCGGTGGTAAAATCATTGTCGCAGTCGATGTGGACGAGGTATTGGGAAACTTTGTCTCGGCTCTCAACCGGTTTATTGCCGATCGTTACTCCTCCCACCATTCAGTTTCAGAGTACCATGTGTATGAGTTCTTCAAGATATGGAACTGTTCACGTGATGAAGCCGATATTCGTGTTCATGAGTTCTTTAAAACATCATATTTCAAGACCGGGATCCACCCACTCCCAGGTGCTCAGGAGACTATTCATAAGTTGTCAGATTTCTGTGACCTGTCAGTTGTGACGTCTCGGCAGAATGCAATTAAGGACCACACCATTCAATGGATCGAACAACATTTTCCAGGGCTCTTTCAGGAGATTCACTTTGGCAACCACTTTGCTTTAAATGGAGAGTCCAGATCTAAGTCAGAAATATGCAGGTCCCTGGGAGCAAAGGTTCTAATTGATGACAATCCAAGATATGCACTTGAATGTGCCGAGGTGGGGATCAGAGTTCTACTTTTTGATTATGAGAACTCATATCCTTGGTGCAAGACCGAGGCTATTGATCAACACCCCCTGGTCACCAGGGTTCATAACTGGGAAGCAGTCGAGAAGCAGCTGATGACAATAAATAGCATAAATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

364

Amino Acids

40.91

Weight (kDa)

7.73

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT5C PF06941 159 - 353 1.2e-13 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33140
fragaria_vesca FvH4_1g09650 FvH4_1g09650
malus_domestica MD02G1105400.v1.1 MD15G1225300.v1.1
prunus_persica Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1
pyrus_communis pycom15g20010
rosa_chinensis RchiOBHm_Chr2g0096491
rosa_laevigata RLG00000016644
rosa_multiflora Rmu_sc0004096.1_g000012
rosa_roxburghii Rroxscaffold_2G00145600
rosa_rugosa Rorug02G0058100
rosa_samantha Rh2AG105100 Rh2BG107200 Rh2CG109000 Rh2DG108200
rosa_wichuraiana Rw2G008120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 749
Acc36I ACCTGC 1 cut(s) 870
AccB7I CCANNNNNTGG 1 cut(s) 783
AccII CGCG 1 cut(s) 182
AciI CCGC 5 cut(s) 104, 164, 180, 182, 379
AclWI GGATC 4 cut(s) 670, 683, 794, 956
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 1 cut(s) 1084
AcvI CACGTG 1 cut(s) 617
AcyI GRCGYC 2 cut(s) 24, 746
AfaI GTAC 2 cut(s) 226, 578
AfiI CCNNNNNNNGG 5 cut(s) 385, 464, 689, 783, 945
AgeI ACCGGT 1 cut(s) 531
AgsI TTSAA 4 cut(s) 598, 667, 782, 932
AjnI CCWGG 7 cut(s) 232, 420, 688, 802, 885, 1020, 1028
AloI GAACNNNNNNTCC 4 cut(s) 360, 392, 882, 914
AluBI AGCT 5 cut(s) 48, 155, 221, 376, 1063
AluI AGCT 5 cut(s) 48, 155, 221, 376, 1063
Alw21I GWGCWC 3 cut(s) 378, 429, 697
Alw26I GTCTC 4 cut(s) 321, 523, 695, 753
AlwI GGATC 4 cut(s) 670, 683, 794, 956
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 4 cut(s) 96, 161, 402, 433
ApeKI GCWGC 5 cut(s) 78, 107, 324, 381, 1060
ApoI RAATTY 1 cut(s) 1084
AsiGI ACCGGT 1 cut(s) 531
AspS9I GGNCC 5 cut(s) 96, 184, 438, 769, 882
AsuC2I CCSGG 5 cut(s) 213, 214, 386, 465, 673
AsuHPI GGTGA 1 cut(s) 1018
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 4 cut(s) 184, 438, 769, 882
BamHI GGATCC 1 cut(s) 675
BanII GRGCYC 2 cut(s) 378, 810
BarI GAAGNNNNNNTAC 2 cut(s) 578, 610
BbrPI CACGTG 1 cut(s) 617
Bbv12I GWGCWC 3 cut(s) 378, 429, 697
BbvI GCAGC 5 cut(s) 65, 94, 336, 393, 1072
BccI CCATC 3 cut(s) 81, 318, 462
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 7 cut(s) 234, 422, 690, 804, 887, 1022, 1030
BclI TGATCA 2 cut(s) 361, 1009
BcnI CCSGG 5 cut(s) 213, 214, 386, 465, 673
BcoDI GTCTC 4 cut(s) 321, 523, 695, 753
BfuAI ACCTGC 1 cut(s) 870
BglI GCCNNNNNGGC 1 cut(s) 104
BglII AGATCT 1 cut(s) 860
BisI GCNGC 7 cut(s) 79, 105, 108, 164, 325, 382, 1061
BlsI GCNGC 7 cut(s) 80, 106, 109, 165, 326, 383, 1062
Bme18I GGWCC 4 cut(s) 184, 438, 769, 882
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 5 cut(s) 96, 184, 438, 769, 882
BmiI GGNNCC 3 cut(s) 97, 677, 884
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 3 cut(s) 122, 307, 379
BmuI ACTGGG 1 cut(s) 1051
BpmI CTGGAG 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 696
BpuMI CCSGG 5 cut(s) 213, 214, 386, 465, 673
BsaAI YACGTR 1 cut(s) 617
BsaBI GATNNNNATC 1 cut(s) 912
BsaHI GRCGYC 2 cut(s) 24, 746
BsaWI WCCGGW 1 cut(s) 531
BsaXI ACNNNNNCTCC 6 cut(s) 51, 81, 360, 390, 882, 912
Bsc4I CCNNNNNNNGG 5 cut(s) 385, 464, 689, 783, 945
Bse118I RCCGGY 2 cut(s) 435, 531
Bse1I ACTGG 2 cut(s) 375, 1046
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 7 cut(s) 234, 422, 690, 804, 887, 1022, 1030
BseGI GGATG 5 cut(s) 137, 301, 337, 394, 454
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 5 cut(s) 385, 464, 689, 783, 945
BseMII CTCAG 4 cut(s) 233, 308, 362, 710
BseNI ACTGG 2 cut(s) 375, 1046
BseRI GAGGAG 3 cut(s) 59, 397, 544
BseXI GCAGC 5 cut(s) 65, 94, 336, 393, 1072
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 2 cut(s) 31, 547
BshFI GGCC 4 cut(s) 98, 163, 404, 435
BshTI ACCGGT 1 cut(s) 531
BsiEI CGRYCG 2 cut(s) 31, 547
BsiHKAI GWGCWC 3 cut(s) 378, 429, 697
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 6 cut(s) 213, 386, 436, 465, 532, 672
BslFI GGGAC 2 cut(s) 154, 868
BslI CCNNNNNNNGG 5 cut(s) 385, 464, 689, 783, 945
BsmAI GTCTC 4 cut(s) 321, 523, 695, 753
BsmBI CGTCTC 1 cut(s) 753
BsmFI GGGAC 2 cut(s) 154, 868
BsmI GAATGC 1 cut(s) 763
BsnI GGCC 4 cut(s) 98, 163, 404, 435
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 4 cut(s) 378, 429, 697, 810
Bsp143I GATC 8 cut(s) 28, 361, 544, 675, 786, 860, 948, 1009
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 5 cut(s) 104, 164, 180, 182, 379
BspANI GGCC 4 cut(s) 98, 163, 404, 435
BspCNI CTCAG 4 cut(s) 234, 307, 361, 709
BspFNI CGCG 1 cut(s) 182
BspHI TCATGA 2 cut(s) 364, 640
BspLI GGNNCC 3 cut(s) 97, 677, 884
BspMI ACCTGC 1 cut(s) 870
BspPI GGATC 4 cut(s) 670, 683, 794, 956
BsrFI RCCGGY 2 cut(s) 435, 531
BsrI ACTGG 2 cut(s) 375, 1046
BssAI RCCGGY 2 cut(s) 435, 531
BssMI GATC 8 cut(s) 28, 361, 544, 675, 786, 860, 948, 1009
BssNI GRCGYC 2 cut(s) 24, 746
BssT1I CCWWGG 2 cut(s) 99, 986
Bst2UI CCWGG 7 cut(s) 234, 422, 690, 804, 887, 1022, 1030
Bst4CI ACNGT 1 cut(s) 611
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 2 cut(s) 24, 746
BstAPI GCANNNNNTGC 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 617
BstC8I GCNNGC 3 cut(s) 46, 83, 287
BstDEI CTNAG 6 cut(s) 242, 294, 348, 696, 864, 1089
BstDSI CCRYGG 2 cut(s) 99, 180
BstEII GGTNACC 1 cut(s) 1024
BstF5I GGATG 5 cut(s) 137, 301, 337, 394, 454
BstFNI CGCG 1 cut(s) 182
BstKTI GATC 8 cut(s) 31, 364, 547, 678, 789, 863, 951, 1012
BstMAI GTCTC 4 cut(s) 321, 523, 695, 753
BstMBI GATC 8 cut(s) 28, 361, 544, 675, 786, 860, 948, 1009
BstMCI CGRYCG 2 cut(s) 31, 547
BstMWI GCNNNNNNNGC 4 cut(s) 30, 104, 261, 295
BstNI CCWGG 7 cut(s) 234, 422, 690, 804, 887, 1022, 1030
BstPI GGTNACC 1 cut(s) 1024
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 5 cut(s) 65, 94, 336, 393, 1072
BstX2I RGATCY 2 cut(s) 675, 860
BstYI RGATCY 2 cut(s) 675, 860
BsuRI GGCC 4 cut(s) 98, 163, 404, 435
BtgI CCRYGG 2 cut(s) 99, 180
BtgZI GCGATG 1 cut(s) 59
BtsCI GGATG 5 cut(s) 137, 301, 337, 394, 454
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 142
BveI ACCTGC 1 cut(s) 870
Cac8I GCNNGC 3 cut(s) 46, 83, 287
CciI TCATGA 2 cut(s) 364, 640
Cfr10I RCCGGY 2 cut(s) 435, 531
Cfr13I GGNCC 5 cut(s) 96, 184, 438, 769, 882
Cfr42I CCGCGG 1 cut(s) 183
Cfr9I CCCGGG 1 cut(s) 212
CseI GACGC 2 cut(s) 13, 323
Csp6I GTAC 2 cut(s) 225, 577
CspAI ACCGGT 1 cut(s) 531
CviAII CATG 5 cut(s) 100, 320, 365, 581, 641
CviQI GTAC 2 cut(s) 225, 577
DdeI CTNAG 6 cut(s) 242, 294, 348, 696, 864, 1089
DpnI GATC 8 cut(s) 30, 363, 546, 677, 788, 862, 950, 1011
DpnII GATC 8 cut(s) 28, 361, 544, 675, 786, 860, 948, 1009
DraI TTTAAA 2 cut(s) 652, 846
EaeI YGGCCR 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 2 cut(s) 99, 986
Eco24I GRGCYC 2 cut(s) 378, 810
Eco47I GGWCC 4 cut(s) 184, 438, 769, 882
Eco53kI GAGCTC 1 cut(s) 376
Eco72I CACGTG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 212
Eco91I GGTNACC 1 cut(s) 1024
EcoICRI GAGCTC 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 882
EcoO65I GGTNACC 1 cut(s) 1024
EcoRII CCWGG 7 cut(s) 232, 420, 688, 802, 885, 1020, 1028
EcoT14I CCWWGG 2 cut(s) 99, 986
EcoT38I GRGCYC 2 cut(s) 378, 810
ErhI CCWWGG 2 cut(s) 99, 986
Esp3I CGTCTC 1 cut(s) 753
FaeI CATG 5 cut(s) 103, 323, 368, 584, 644
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 2 cut(s) 154, 868
FatI CATG 5 cut(s) 99, 319, 364, 580, 640
FbaI TGATCA 2 cut(s) 361, 1009
Fnu4HI GCNGC 7 cut(s) 79, 105, 108, 164, 325, 382, 1061
FokI GGATG 5 cut(s) 144, 288, 324, 401, 441
FriOI GRGCYC 2 cut(s) 378, 810
Fsp4HI GCNGC 7 cut(s) 79, 105, 108, 164, 325, 382, 1061
GluI GCNGC 7 cut(s) 79, 105, 108, 164, 325, 382, 1061
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 4 cut(s) 98, 163, 404, 435
HapII CCGG 6 cut(s) 213, 386, 436, 465, 532, 672
HgaI GACGC 2 cut(s) 13, 323
Hin1I GRCGYC 2 cut(s) 24, 746
Hin1II CATG 5 cut(s) 103, 323, 368, 584, 644
HinfI GANTC 4 cut(s) 238, 444, 820, 854
HpaII CCGG 6 cut(s) 213, 386, 436, 465, 532, 672
HphI GGTGA 1 cut(s) 1018
Hpy166II GTNNAC 2 cut(s) 496, 614
Hpy188I TCNGA 5 cut(s) 243, 574, 721, 871, 953
Hpy8I GTNNAC 2 cut(s) 496, 614
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 9, 271
HpyCH4III ACNGT 1 cut(s) 611
HpyCH4IV ACGT 2 cut(s) 616, 746
HpyCH4V TGCA 8 cut(s) 135, 195, 264, 289, 761, 879, 926, 993
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 104, 261, 295
HpyF3I CTNAG 6 cut(s) 242, 294, 348, 696, 864, 1089
HpySE526I ACGT 2 cut(s) 616, 746
Hsp92I GRCGYC 2 cut(s) 24, 746
Hsp92II CATG 5 cut(s) 103, 323, 368, 584, 644
Ksp22I TGATCA 2 cut(s) 361, 1009
KspI CCGCGG 1 cut(s) 183
Kzo9I GATC 8 cut(s) 28, 361, 544, 675, 786, 860, 948, 1009
LmnI GCTCC 5 cut(s) 72, 373, 381, 424, 890
Lsp1109I GCAGC 5 cut(s) 65, 94, 336, 393, 1072
LweI GCATC 3 cut(s) 122, 307, 379
MaeII ACGT 2 cut(s) 616, 746
MaeIII GTNAC 4 cut(s) 548, 728, 742, 1024
MalI GATC 8 cut(s) 30, 363, 546, 677, 788, 862, 950, 1011
MboI GATC 8 cut(s) 28, 361, 544, 675, 786, 860, 948, 1009
MboII GAAGA 2 cut(s) 31, 586
MflI RGATCY 2 cut(s) 675, 860
MhlI GDGCHC 4 cut(s) 378, 429, 697, 810
MluCI AATT 5 cut(s) 205, 265, 762, 903, 1084
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 1 cut(s) 376
MseI TTAA 4 cut(s) 330, 651, 765, 845
MslI CAYNNNNRTG 3 cut(s) 342, 585, 931
MspA1I CMGCKG 2 cut(s) 182, 1063
MspI CCGG 6 cut(s) 213, 386, 436, 465, 532, 672
Mva1269I GAATGC 1 cut(s) 763
MvaI CCWGG 7 cut(s) 234, 422, 690, 804, 887, 1022, 1030
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 30, 104, 261, 295
NciI CCSGG 5 cut(s) 213, 214, 386, 465, 673
NcoI CCATGG 1 cut(s) 99
NdeII GATC 8 cut(s) 28, 361, 544, 675, 786, 860, 948, 1009
NlaIII CATG 5 cut(s) 103, 323, 368, 584, 644
NlaIV GGNNCC 3 cut(s) 97, 677, 884
NmeAIII GCCGAG 3 cut(s) 500, 730, 964
NmuCI GTSAC 3 cut(s) 728, 742, 1024
PagI TCATGA 2 cut(s) 364, 640
PasI CCCWGGG 1 cut(s) 886
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PctI GAATGC 1 cut(s) 763
PfeI GAWTC 3 cut(s) 238, 444, 820
PflMI CCANNNNNTGG 1 cut(s) 783
PinAI ACCGGT 1 cut(s) 531
PkrI GCNGC 7 cut(s) 80, 106, 109, 165, 326, 383, 1062
Ple19I CGATCG 2 cut(s) 31, 547
PleI GAGTC 1 cut(s) 862
PmaCI CACGTG 1 cut(s) 617
PmlI CACGTG 1 cut(s) 617
PpsI GAGTC 1 cut(s) 862
Ppu21I YACGTR 1 cut(s) 617
PpuMI RGGWCCY 1 cut(s) 882
Psp124BI GAGCTC 1 cut(s) 378
Psp5II RGGWCCY 1 cut(s) 882
Psp6I CCWGG 7 cut(s) 232, 420, 688, 802, 885, 1020, 1028
PspCI CACGTG 1 cut(s) 617
PspEI GGTNACC 1 cut(s) 1024
PspGI CCWGG 7 cut(s) 232, 420, 688, 802, 885, 1020, 1028
PspN4I GGNNCC 3 cut(s) 97, 677, 884
PspPI GGNCC 5 cut(s) 96, 184, 438, 769, 882
PspPPI RGGWCCY 1 cut(s) 882
PsuI RGATCY 2 cut(s) 675, 860
PvuI CGATCG 2 cut(s) 31, 547
PvuII CAGCTG 1 cut(s) 1063
RsaI GTAC 2 cut(s) 226, 578
RsaNI GTAC 2 cut(s) 225, 577
RseI CAYNNNNRTG 3 cut(s) 342, 585, 931
SacI GAGCTC 1 cut(s) 378
SacII CCGCGG 1 cut(s) 183
SaqAI TTAA 4 cut(s) 330, 651, 765, 845
SatI GCNGC 7 cut(s) 79, 105, 108, 164, 325, 382, 1061
Sau3AI GATC 8 cut(s) 28, 361, 544, 675, 786, 860, 948, 1009
Sau96I GGNCC 5 cut(s) 96, 184, 438, 769, 882
SchI GAGTC 1 cut(s) 863
SduI GDGCHC 4 cut(s) 378, 429, 697, 810
SfaNI GCATC 3 cut(s) 122, 307, 379
Sfr303I CCGCGG 1 cut(s) 183
SgrBI CCGCGG 1 cut(s) 183
SinI GGWCC 4 cut(s) 184, 438, 769, 882
SmaI CCCGGG 1 cut(s) 214
SmiMI CAYNNNNRTG 3 cut(s) 342, 585, 931
Sse9I AATT 5 cut(s) 205, 265, 762, 903, 1084
SsiI CCGC 5 cut(s) 104, 164, 180, 182, 379
SstI GAGCTC 1 cut(s) 378
StyI CCWWGG 2 cut(s) 99, 986
TaaI ACNGT 1 cut(s) 611
TaiI ACGT 2 cut(s) 619, 749
TaqI TCGA 4 cut(s) 27, 489, 789, 1053
TaqII GACCGA 1 cut(s) 1013
TasI AATT 5 cut(s) 205, 265, 762, 903, 1084
TauI GCSGC 2 cut(s) 107, 166
TfiI GAWTC 3 cut(s) 238, 444, 820
Tru1I TTAA 4 cut(s) 330, 651, 765, 845
Tru9I TTAA 4 cut(s) 330, 651, 765, 845
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 3 cut(s) 728, 742, 1024
TseI GCWGC 5 cut(s) 78, 107, 324, 381, 1060
Tsp45I GTSAC 3 cut(s) 728, 742, 1024
TspDTI ATGAA 6 cut(s) 134, 381, 629, 636, 698, 1025
TspGWI ACGGA 1 cut(s) 444
TspMI CCCGGG 1 cut(s) 212
TspRI CASTG 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 783
VpaK11BI GGWCC 4 cut(s) 184, 438, 769, 882
XapI RAATTY 1 cut(s) 1084
XcmI CCANNNNNNNNNTGG 2 cut(s) 317, 349
XmaI CCCGGG 1 cut(s) 212
ZraI GACGTC 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.