MD02G1105400.v1.1

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012369
Physical Location & Seq
Reverse (-)
8547877 .. 8550853
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1105400.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGTTGTTGTTGAAAAGGCAAGGCGTCGATCGCTTCCTCTTCGCAAGCTCAAAGCACCGACCTTTGCTCCTCCATTGCCGCCAGCAGTATACAGAGGCCATGGCGGCTGCTTTTTTGTTTAGCCATAGTGGATGCAGAGGGTTTCAAACCCAAGCTCAATGGACGCCTTCCCATTTTTCTGCGGGCCATTCTTGCAAGTCTGCGAATTTTCCCGGGAAAGCTGGAATTTTTCCTGGGAATCTGAGGAATACAAGCATTGGGTGCAATTTTGTTGTGAAGGGGTGCTTACAATCTCAGCATCATCCCCACAATCAGTCTCATAGCAATAGCATTAAGCGTCATCCCAATGCTCAGTTGGTTCGTGATCTTGAGCTGGACCTCCGCAGCTCGGATACCGTTGGATTGACCTCCTCAAATGGTTGCCAGGAGCGCGGAGGCCGGGCCGGAATCCCACACCATCCTTTGCCCGGTGATAAACTCGTTGTCGCGGTCGATGTGGACGAGGTATTGGGAAACTTTATCTCGGCTCTCAACTGCTTTATTGCTGATCGTTACTCGTCCAACCATTCAGTTTCGGAGTACCATGTGTATGAGTTCTTCAAGATATGGAACTGTTCACGTGATGAAGCCGATTATCGTGTTCACGAGTTCTTTAAATCATCATATTTCAAGACCGGGATCCATCCACTCCCAGGTGCTCAGGAGACGATTCATAAGTTGTCAGATTTCTGTGACCTGTCGGTTGTGACGTCTCGGCAGAATGTAATTAAGGACCACACTCTTCAATGGATCGAACAACATTTTTCAGGGCTGTTTCAGGAAATTCACTTTGGCAACCACTGGGCTTTGCATGGAGAGTCCAGGCCTAAGTCAGAAATATGCAGGTCCCTGGGAGCAAAGGTTCTAATCGATGACAATCCAAGATATGCACTTGAATGTGCCGAGGTGGGGATCAGGGTTCTACTTTTTGATTATGAGAACTCATATCCCTGGTGCAAGACGGAGTCTATTGATCAACACCCCCTGGTCACCAAGGTTCATAACTGGGAAGCTGTCGAGAAGCAGCTGATGACCATAAACAGCATAATTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

365

Amino Acids

41.2

Weight (kDa)

7.35

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT5C PF06941 160 - 325 6.8e-12 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33140
fragaria_vesca FvH4_1g09650 FvH4_1g09650
malus_domestica MD02G1105400.v1.1 MD15G1225300.v1.1
prunus_persica Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1
pyrus_communis pycom15g20010
rosa_chinensis RchiOBHm_Chr2g0096491
rosa_laevigata RLG00000016644
rosa_multiflora Rmu_sc0004096.1_g000012
rosa_roxburghii Rroxscaffold_2G00145600
rosa_rugosa Rorug02G0058100
rosa_samantha Rh2AG105100 Rh2BG107200 Rh2CG109000 Rh2DG108200
rosa_wichuraiana Rw2G008120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 752
Acc36I ACCTGC 1 cut(s) 873
AccI GTMKAC 1 cut(s) 89
AccII CGCG 2 cut(s) 432, 488
AciI CCGC 6 cut(s) 79, 104, 182, 382, 432, 488
AclWI GGATC 4 cut(s) 673, 686, 797, 959
AcsI RAATTY 3 cut(s) 205, 225, 822
AcvI CACGTG 1 cut(s) 620
AcyI GRCGYC 3 cut(s) 24, 164, 749
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 4 cut(s) 388, 467, 692, 948
AgsI TTSAA 6 cut(s) 13, 146, 601, 670, 785, 935
AjnI CCWGG 7 cut(s) 232, 423, 691, 860, 888, 989, 1023
AjuI GAANNNNNNNTTGG 2 cut(s) 813, 845
AloI GAACNNNNNNTCC 2 cut(s) 885, 917
AluBI AGCT 7 cut(s) 48, 155, 221, 373, 387, 1052, 1066
AluI AGCT 7 cut(s) 48, 155, 221, 373, 387, 1052, 1066
Alw21I GWGCWC 1 cut(s) 700
Alw26I GTCTC 3 cut(s) 321, 698, 756
AlwI GGATC 4 cut(s) 673, 686, 797, 959
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 5 cut(s) 96, 184, 436, 441, 863
ApeKI GCWGC 3 cut(s) 107, 384, 1063
ApoI RAATTY 3 cut(s) 205, 225, 822
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 5 cut(s) 184, 376, 441, 772, 885
AsuC2I CCSGG 5 cut(s) 213, 214, 440, 468, 676
AsuHPI GGTGA 2 cut(s) 482, 1021
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 3 cut(s) 376, 772, 885
BamHI GGATCC 1 cut(s) 678
BarI GAAGNNNNNNTAC 2 cut(s) 581, 613
BauI CACGAG 1 cut(s) 644
BbrPI CACGTG 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 700
BbvI GCAGC 3 cut(s) 94, 396, 1075
BccI CCATC 2 cut(s) 465, 690
BciT130I CCWGG 7 cut(s) 234, 425, 693, 862, 890, 991, 1025
BciVI GTATCC 1 cut(s) 385
BclI TGATCA 1 cut(s) 1012
BcnI CCSGG 5 cut(s) 213, 214, 440, 468, 676
BcoDI GTCTC 3 cut(s) 321, 698, 756
BfuAI ACCTGC 1 cut(s) 873
BfuI GTATCC 1 cut(s) 385
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 5 cut(s) 79, 105, 108, 385, 1064
BlsI GCNGC 5 cut(s) 80, 106, 109, 386, 1065
Bme18I GGWCC 3 cut(s) 376, 772, 885
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 5 cut(s) 184, 376, 441, 772, 885
BmiI GGNNCC 2 cut(s) 680, 887
BmrI ACTGGG 2 cut(s) 850, 1054
BmsI GCATC 2 cut(s) 122, 307
BmuI ACTGGG 2 cut(s) 850, 1054
Bpu10I CCTNAGC 1 cut(s) 699
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 5 cut(s) 213, 214, 440, 468, 676
Bsa29I ATCGAT 1 cut(s) 909
BsaAI YACGTR 1 cut(s) 620
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 3 cut(s) 24, 164, 749
BsaXI ACNNNNNCTCC 4 cut(s) 51, 81, 885, 915
Bsc4I CCNNNNNNNGG 4 cut(s) 388, 467, 692, 948
Bse1I ACTGG 2 cut(s) 845, 1049
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 7 cut(s) 234, 425, 693, 862, 890, 991, 1025
BseCI ATCGAT 1 cut(s) 909
BseGI GGATG 5 cut(s) 137, 301, 340, 457, 682
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 4 cut(s) 388, 467, 692, 948
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 4 cut(s) 233, 308, 365, 713
BseNI ACTGG 2 cut(s) 845, 1049
BseRI GAGGAG 2 cut(s) 59, 400
BseXI GCAGC 3 cut(s) 94, 396, 1075
Bsh1236I CGCG 2 cut(s) 432, 488
Bsh1285I CGRYCG 2 cut(s) 31, 492
BshFI GGCC 5 cut(s) 98, 186, 438, 443, 865
BshVI ATCGAT 1 cut(s) 909
BsiEI CGRYCG 2 cut(s) 31, 492
BsiHKAI GWGCWC 1 cut(s) 700
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 5 cut(s) 213, 439, 444, 468, 675
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 4 cut(s) 388, 467, 692, 948
BsmAI GTCTC 3 cut(s) 321, 698, 756
BsmBI CGTCTC 2 cut(s) 698, 756
BsmFI GGGAC 1 cut(s) 871
BsnI GGCC 5 cut(s) 98, 186, 438, 443, 865
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 700
Bsp143I GATC 7 cut(s) 28, 364, 547, 678, 789, 951, 1012
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 6 cut(s) 79, 104, 182, 382, 432, 488
BspANI GGCC 5 cut(s) 98, 186, 438, 443, 865
BspCNI CTCAG 4 cut(s) 234, 307, 364, 712
BspDI ATCGAT 1 cut(s) 909
BspFNI CGCG 2 cut(s) 432, 488
BspLI GGNNCC 2 cut(s) 680, 887
BspMI ACCTGC 1 cut(s) 873
BspPI GGATC 4 cut(s) 673, 686, 797, 959
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 2 cut(s) 845, 1049
BssMI GATC 7 cut(s) 28, 364, 547, 678, 789, 951, 1012
BssNAI GTATAC 1 cut(s) 90
BssNI GRCGYC 3 cut(s) 24, 164, 749
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 2 cut(s) 99, 1032
Bst1107I GTATAC 1 cut(s) 90
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 7 cut(s) 234, 425, 693, 862, 890, 991, 1025
Bst4CI ACNGT 2 cut(s) 397, 614
Bst6I CTCTTC 2 cut(s) 44, 786
BstACI GRCGYC 3 cut(s) 24, 164, 749
BstAPI GCANNNNNTGC 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 620
BstC8I GCNNGC 3 cut(s) 46, 83, 184
BstDEI CTNAG 5 cut(s) 242, 294, 351, 699, 867
BstDSI CCRYGG 1 cut(s) 99
BstEII GGTNACC 1 cut(s) 1027
BstF5I GGATG 5 cut(s) 137, 301, 340, 457, 682
BstFNI CGCG 2 cut(s) 432, 488
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 7 cut(s) 31, 367, 550, 681, 792, 954, 1015
BstMAI GTCTC 3 cut(s) 321, 698, 756
BstMBI GATC 7 cut(s) 28, 364, 547, 678, 789, 951, 1012
BstMCI CGRYCG 2 cut(s) 31, 492
BstMWI GCNNNNNNNGC 5 cut(s) 30, 104, 192, 261, 429
BstNI CCWGG 7 cut(s) 234, 425, 693, 862, 890, 991, 1025
BstPI GGTNACC 1 cut(s) 1027
BstUI CGCG 2 cut(s) 432, 488
BstV1I GCAGC 3 cut(s) 94, 396, 1075
BstX2I RGATCY 1 cut(s) 678
BstYI RGATCY 1 cut(s) 678
BstZ17I GTATAC 1 cut(s) 90
Bsu15I ATCGAT 1 cut(s) 909
BsuI GTATCC 1 cut(s) 385
BsuRI GGCC 5 cut(s) 98, 186, 438, 443, 865
BsuTUI ATCGAT 1 cut(s) 909
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 5 cut(s) 137, 301, 340, 457, 682
BtsIMutI CAGTG 1 cut(s) 838
BveI ACCTGC 1 cut(s) 873
Cac8I GCNNGC 3 cut(s) 46, 83, 184
CfoI GCGC 1 cut(s) 432
Cfr13I GGNCC 5 cut(s) 184, 376, 441, 772, 885
Cfr9I CCCGGG 1 cut(s) 212
ClaI ATCGAT 1 cut(s) 909
CseI GACGC 3 cut(s) 13, 172, 326
Csp6I GTAC 1 cut(s) 580
CviAII CATG 3 cut(s) 100, 584, 851
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 5 cut(s) 242, 294, 351, 699, 867
DpnI GATC 7 cut(s) 30, 366, 549, 680, 791, 953, 1014
DpnII GATC 7 cut(s) 28, 364, 547, 678, 789, 951, 1012
DraI TTTAAA 1 cut(s) 655
Eam1104I CTCTTC 2 cut(s) 44, 786
EarI CTCTTC 2 cut(s) 44, 786
Eco130I CCWWGG 2 cut(s) 99, 1032
Eco147I AGGCCT 1 cut(s) 865
Eco47I GGWCC 3 cut(s) 376, 772, 885
Eco72I CACGTG 1 cut(s) 620
Eco88I CYCGRG 1 cut(s) 212
Eco91I GGTNACC 1 cut(s) 1027
EcoO109I RGGNCCY 1 cut(s) 885
EcoO65I GGTNACC 1 cut(s) 1027
EcoRII CCWGG 7 cut(s) 232, 423, 691, 860, 888, 989, 1023
EcoT14I CCWWGG 2 cut(s) 99, 1032
ErhI CCWWGG 2 cut(s) 99, 1032
Esp3I CGTCTC 2 cut(s) 698, 756
FaeI CATG 3 cut(s) 103, 587, 854
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 871
FatI CATG 3 cut(s) 99, 583, 850
FauI CCCGC 1 cut(s) 175
FbaI TGATCA 1 cut(s) 1012
FblI GTMKAC 1 cut(s) 89
Fnu4HI GCNGC 5 cut(s) 79, 105, 108, 385, 1064
FokI GGATG 5 cut(s) 144, 288, 327, 444, 669
Fsp4HI GCNGC 5 cut(s) 79, 105, 108, 385, 1064
GlaI GCGC 1 cut(s) 431
GluI GCNGC 5 cut(s) 79, 105, 108, 385, 1064
HaeIII GGCC 5 cut(s) 98, 186, 438, 443, 865
HapII CCGG 5 cut(s) 213, 439, 444, 468, 675
HgaI GACGC 3 cut(s) 13, 172, 326
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 3 cut(s) 24, 164, 749
Hin1II CATG 3 cut(s) 103, 587, 854
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HinfI GANTC 5 cut(s) 238, 447, 709, 857, 1004
HpaII CCGG 5 cut(s) 213, 439, 444, 468, 675
HphI GGTGA 2 cut(s) 482, 1021
Hpy166II GTNNAC 4 cut(s) 90, 499, 617, 643
Hpy188I TCNGA 5 cut(s) 243, 391, 577, 724, 874
Hpy188III TCNNGA 8 cut(s) 362, 368, 601, 644, 670, 701, 818, 1057
Hpy8I GTNNAC 4 cut(s) 90, 499, 617, 643
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 177, 271
HpyCH4III ACNGT 2 cut(s) 397, 614
HpyCH4IV ACGT 2 cut(s) 619, 749
HpyCH4V TGCA 7 cut(s) 135, 195, 264, 850, 882, 929, 996
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 104, 192, 261, 429
HpyF3I CTNAG 5 cut(s) 242, 294, 351, 699, 867
HpySE526I ACGT 2 cut(s) 619, 749
Hsp92I GRCGYC 3 cut(s) 24, 164, 749
Hsp92II CATG 3 cut(s) 103, 587, 854
HspAI GCGC 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 1012
Kzo9I GATC 7 cut(s) 28, 364, 547, 678, 789, 951, 1012
LmnI GCTCC 3 cut(s) 72, 427, 893
Lsp1109I GCAGC 3 cut(s) 94, 396, 1075
LweI GCATC 2 cut(s) 122, 307
MaeII ACGT 2 cut(s) 619, 749
MaeIII GTNAC 4 cut(s) 551, 731, 745, 1027
MalI GATC 7 cut(s) 30, 366, 549, 680, 791, 953, 1014
MboI GATC 7 cut(s) 28, 364, 547, 678, 789, 951, 1012
MboII GAAGA 3 cut(s) 31, 589, 773
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 1 cut(s) 700
MluCI AATT 6 cut(s) 205, 225, 265, 765, 822, 1086
MlyI GAGTC 2 cut(s) 866, 1013
MmeI TCCRAC 2 cut(s) 379, 585
MseI TTAA 3 cut(s) 333, 654, 768
MslI CAYNNNNRTG 3 cut(s) 345, 588, 934
MspA1I CMGCKG 1 cut(s) 1066
MspI CCGG 5 cut(s) 213, 439, 444, 468, 675
MvaI CCWGG 7 cut(s) 234, 425, 693, 862, 890, 991, 1025
MvnI CGCG 2 cut(s) 432, 488
MwoI GCNNNNNNNGC 5 cut(s) 30, 104, 192, 261, 429
NciI CCSGG 5 cut(s) 213, 214, 440, 468, 676
NcoI CCATGG 1 cut(s) 99
NdeII GATC 7 cut(s) 28, 364, 547, 678, 789, 951, 1012
NlaIII CATG 3 cut(s) 103, 587, 854
NlaIV GGNNCC 2 cut(s) 680, 887
NmeAIII GCCGAG 3 cut(s) 503, 733, 967
NmuCI GTSAC 3 cut(s) 731, 745, 1027
PasI CCCWGGG 1 cut(s) 889
PceI AGGCCT 1 cut(s) 865
PcsI WCGNNNNNNNCGW 2 cut(s) 498, 746
PfeI GAWTC 3 cut(s) 238, 447, 709
PflFI GACNNNGTC 1 cut(s) 1003
PkrI GCNGC 5 cut(s) 80, 106, 109, 386, 1065
Ple19I CGATCG 1 cut(s) 31
PleI GAGTC 2 cut(s) 865, 1012
PmaCI CACGTG 1 cut(s) 620
PmlI CACGTG 1 cut(s) 620
PpsI GAGTC 2 cut(s) 865, 1012
Ppu21I YACGTR 1 cut(s) 620
PpuMI RGGWCCY 1 cut(s) 885
Psp5II RGGWCCY 1 cut(s) 885
Psp6I CCWGG 7 cut(s) 232, 423, 691, 860, 888, 989, 1023
PspCI CACGTG 1 cut(s) 620
PspEI GGTNACC 1 cut(s) 1027
PspGI CCWGG 7 cut(s) 232, 423, 691, 860, 888, 989, 1023
PspN4I GGNNCC 2 cut(s) 680, 887
PspPI GGNCC 5 cut(s) 184, 376, 441, 772, 885
PspPPI RGGWCCY 1 cut(s) 885
PsuI RGATCY 1 cut(s) 678
PsyI GACNNNGTC 1 cut(s) 1003
PvuI CGATCG 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 1066
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
RseI CAYNNNNRTG 3 cut(s) 345, 588, 934
SaqAI TTAA 3 cut(s) 333, 654, 768
SatI GCNGC 5 cut(s) 79, 105, 108, 385, 1064
Sau3AI GATC 7 cut(s) 28, 364, 547, 678, 789, 951, 1012
Sau96I GGNCC 5 cut(s) 184, 376, 441, 772, 885
SchI GAGTC 2 cut(s) 866, 1013
SduI GDGCHC 1 cut(s) 700
SfaNI GCATC 2 cut(s) 122, 307
SinI GGWCC 3 cut(s) 376, 772, 885
SmaI CCCGGG 1 cut(s) 214
SmiMI CAYNNNNRTG 3 cut(s) 345, 588, 934
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 6 cut(s) 205, 225, 265, 765, 822, 1086
SseBI AGGCCT 1 cut(s) 865
SsiI CCGC 6 cut(s) 79, 104, 182, 382, 432, 488
StuI AGGCCT 1 cut(s) 865
StyI CCWWGG 2 cut(s) 99, 1032
TaaI ACNGT 2 cut(s) 397, 614
TaiI ACGT 2 cut(s) 622, 752
TaqI TCGA 5 cut(s) 27, 492, 792, 909, 1056
TasI AATT 6 cut(s) 205, 225, 265, 765, 822, 1086
TauI GCSGC 2 cut(s) 81, 107
TfiI GAWTC 3 cut(s) 238, 447, 709
Tru1I TTAA 3 cut(s) 333, 654, 768
Tru9I TTAA 3 cut(s) 333, 654, 768
TscAI CASTG 1 cut(s) 845
TseFI GTSAC 3 cut(s) 731, 745, 1027
TseI GCWGC 3 cut(s) 107, 384, 1063
Tsp45I GTSAC 3 cut(s) 731, 745, 1027
TspDTI ATGAA 4 cut(s) 639, 701, 1028, 1080
TspGWI ACGGA 1 cut(s) 1016
TspMI CCCGGG 1 cut(s) 212
TspRI CASTG 1 cut(s) 845
Tth111I GACNNNGTC 1 cut(s) 1003
VpaK11BI GGWCC 3 cut(s) 376, 772, 885
XapI RAATTY 3 cut(s) 205, 225, 822
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmaI CCCGGG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 89
ZraI GACGTC 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.