Rw2G008120

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012369
Physical Location & Seq
Reverse (-)
8059248 .. 8061529
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G008120.1

Sequence Viewer

Length: 1005 bp
ATGTTGGTGTTGAAAAGGCAAGGCGCCGTTAACTTTCTCACACACCCTTCAAAGCACCACCAACCTTTGAGTCTAGGTCGTCGTCTTCATCGATTTACAGAGATGGCCGCCTATCTGTACAACCATAACTGTAAGGGGTTTCACGCACAAGCCCCAATTCCGCACCCAAATCATCGATTCGCGGGTCTTGTTCGCCAGATTCGGAATCGAAAGACGGCCAATTTGACCGTGAAATGTTGCTTCAATAACAATAATAGAGTGAAGGGCAATGTGTTTGAGAATCCGAATGGGCGGTTGGTCCGGGATTTGGATTCGGAGCTCCGGAGATCCCAGAACGGTGGCTTTCAGGGGAGTCTTAATGGGTTTCACAGCCAGACTCTGTCCCGGGATAAAATTGTGGTTGCTGTTGATGTGGATGAGGTTTTGGGCAACTTTGTGTCGGCTCTGAACAGGTTTATTGCTGATCGTTACGCGGCGAACCATACGGTTTCGGAGTACCATGTGTATGAGTTCTTCAAGATATGGAAGTGTTCCCGTGATGAAGCCGATCTTCGTGTTCATGAGTTCTTTAAAACATCATATTTCAAGACTGGGATCCACCCACTCCCAGGTGCTAAGGAGACGCTTAATAAGTTGTCAGGATTTTGTAACCTGTCAGTTGTGACTTCTCGGCAGAATGCAATCAAGGACCACACAATTCAATGGATTGAAAGTCATTTTCCAGGACTATTTCAGGAGATTCACTTTGGCAACCACTTTGCTCTACATGGAGAGTCAAGGCCCAAGTCTGAAATATGCAGGTCTCTAGGAGCAAAGGTTCTGATTGATGACAATCCAAGGTATGCACTTGAATGTGCCGAAGTTGGGATCAGGGTTCTACTTTTTGATTACGAGAACTCATATCCTTGGTGCAAGTCCGATTCTGTTGATCTACATCCCCTCGTGACCAGGGTTCATAACTGGGAAGCAGTGGAGAAGCAATTATTGGCATGGGTTACTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

334

Amino Acids

38.33

Weight (kDa)

9.23

Isoelectric Point (pI)

26.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT5C PF06941 132 - 326 1.7e-14 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33140
fragaria_vesca FvH4_1g09650 FvH4_1g09650
malus_domestica MD02G1105400.v1.1 MD15G1225300.v1.1
prunus_persica Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1
pyrus_communis pycom15g20010
rosa_chinensis RchiOBHm_Chr2g0096491
rosa_laevigata RLG00000016644
rosa_multiflora Rmu_sc0004096.1_g000012
rosa_roxburghii Rroxscaffold_2G00145600
rosa_rugosa Rorug02G0058100
rosa_samantha Rh2AG105100 Rh2BG107200 Rh2CG109000 Rh2DG108200
rosa_wichuraiana Rw2G008120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 789
AccB1I GGYRCC 1 cut(s) 23
AccII CGCG 2 cut(s) 182, 473
AccIII TCCGGA 1 cut(s) 321
AciI CCGC 5 cut(s) 108, 161, 182, 292, 473
AclWI GGATC 4 cut(s) 321, 589, 602, 875
AcoI YGGCCR 2 cut(s) 105, 216
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 2 cut(s) 119, 497
AfiI CCNNNNNNNGG 3 cut(s) 307, 608, 864
AgsI TTSAA 8 cut(s) 13, 51, 244, 517, 586, 701, 710, 851
AjnI CCWGG 3 cut(s) 607, 721, 947
AjuI GAANNNNNNNTTGG 4 cut(s) 278, 310, 968, 1000
AloI GAACNNNNNNTCC 2 cut(s) 801, 833
AluBI AGCT 1 cut(s) 319
AluI AGCT 1 cut(s) 319
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 614, 807
AlwI GGATC 4 cut(s) 321, 589, 602, 875
AlwNI CAGNNNCTG 1 cut(s) 379
Ama87I CYCGRG 1 cut(s) 384
Aor13HI TCCGGA 1 cut(s) 321
AoxI GGCC 3 cut(s) 105, 216, 779
ArsI GACNNNNNNTTYG 2 cut(s) 205, 237
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 3 cut(s) 298, 688, 780
AsuC2I CCSGG 3 cut(s) 302, 385, 386
AvaI CYCGRG 1 cut(s) 384
AvaII GGWCC 2 cut(s) 298, 688
BamHI GGATCC 1 cut(s) 594
BanI GGYRCC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 321
BarI GAAGNNNNNNTAC 2 cut(s) 497, 529
BauI CACGAG 1 cut(s) 941
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 321
BccI CCATC 1 cut(s) 97
BceAI ACGGC 2 cut(s) 11, 231
BciT130I CCWGG 3 cut(s) 609, 723, 949
BcnI CCSGG 3 cut(s) 302, 385, 386
BcoDI GTCTC 2 cut(s) 614, 807
BfaI CTAG 2 cut(s) 74, 806
BfoI RGCGCY 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 789
BisI GCNGC 2 cut(s) 108, 474
BlsI GCNGC 2 cut(s) 109, 475
Bme1390I CCNGG 6 cut(s) 302, 385, 386, 609, 723, 949
Bme18I GGWCC 2 cut(s) 298, 688
BmeT110I CYCGRG 1 cut(s) 384
BmgT120I GGNCC 3 cut(s) 298, 688, 780
BmiI GGNNCC 2 cut(s) 25, 596
BmrFI CCNGG 6 cut(s) 302, 385, 386, 609, 723, 949
BmrI ACTGGG 2 cut(s) 600, 970
BmuI ACTGGG 2 cut(s) 600, 970
BpiI GAAGAC 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 615
BpuMI CCSGG 3 cut(s) 302, 385, 386
Bsa29I ATCGAT 2 cut(s) 91, 175
BsaBI GATNNNNATC 2 cut(s) 831, 933
BsaHI GRCGYC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 807
BsaJI CCNNGG 5 cut(s) 384, 607, 836, 905, 948
BsaWI WCCGGW 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 608, 864
Bse1I ACTGG 2 cut(s) 595, 965
Bse3DI GCAATG 1 cut(s) 274
Bse8I GATNNNNATC 2 cut(s) 831, 933
BseAI TCCGGA 1 cut(s) 321
BseBI CCWGG 3 cut(s) 609, 723, 949
BseCI ATCGAT 2 cut(s) 91, 175
BseDI CCNNGG 5 cut(s) 384, 607, 836, 905, 948
BseGI GGATG 2 cut(s) 421, 934
BseJI GATNNNNATC 2 cut(s) 831, 933
BseLI CCNNNNNNNGG 3 cut(s) 307, 608, 864
BseMI GCAATG 1 cut(s) 274
BseNI ACTGG 2 cut(s) 595, 965
Bsh1236I CGCG 2 cut(s) 182, 473
BshFI GGCC 3 cut(s) 107, 218, 781
BshNI GGYRCC 1 cut(s) 23
BshVI ATCGAT 2 cut(s) 91, 175
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 384
BsiSI CCGG 3 cut(s) 301, 322, 385
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 307, 608, 864
BsmAI GTCTC 2 cut(s) 614, 807
BsmBI CGTCTC 1 cut(s) 614
BsmFI GGGAC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 3 cut(s) 107, 218, 781
Bso31I GGTCTC 1 cut(s) 807
BsoBI CYCGRG 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 321
Bsp13I TCCGGA 1 cut(s) 321
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 6 cut(s) 326, 463, 547, 594, 867, 928
BspACI CCGC 5 cut(s) 108, 161, 182, 292, 473
BspANI GGCC 3 cut(s) 107, 218, 781
BspDI ATCGAT 2 cut(s) 91, 175
BspEI TCCGGA 1 cut(s) 321
BspFNI CGCG 2 cut(s) 182, 473
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 2 cut(s) 25, 596
BspMI ACCTGC 1 cut(s) 789
BspPI GGATC 4 cut(s) 321, 589, 602, 875
BspT107I GGYRCC 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 807
BsrDI GCAATG 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 117
BsrI ACTGG 2 cut(s) 595, 965
BssECI CCNNGG 5 cut(s) 384, 607, 836, 905, 948
BssMI GATC 6 cut(s) 326, 463, 547, 594, 867, 928
BssNI GRCGYC 1 cut(s) 24
BssSI CACGAG 1 cut(s) 941
BssT1I CCWWGG 2 cut(s) 836, 905
Bst2BI CACGAG 1 cut(s) 941
Bst2UI CCWGG 3 cut(s) 609, 723, 949
Bst4CI ACNGT 4 cut(s) 131, 229, 338, 487
BstACI GRCGYC 1 cut(s) 24
BstAUI TGTACA 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 615
BstF5I GGATG 2 cut(s) 421, 934
BstFNI CGCG 2 cut(s) 182, 473
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 6 cut(s) 329, 466, 550, 597, 870, 931
BstMAI GTCTC 2 cut(s) 614, 807
BstMBI GATC 6 cut(s) 326, 463, 547, 594, 867, 928
BstNI CCWGG 3 cut(s) 609, 723, 949
BstSCI CCNGG 6 cut(s) 300, 383, 384, 607, 721, 947
BstUI CGCG 2 cut(s) 182, 473
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 2 cut(s) 326, 594
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 2 cut(s) 326, 594
Bsu15I ATCGAT 2 cut(s) 91, 175
BsuRI GGCC 3 cut(s) 107, 218, 781
BsuTUI ATCGAT 2 cut(s) 91, 175
BtsCI GGATG 2 cut(s) 421, 934
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 1 cut(s) 975
BveI ACCTGC 1 cut(s) 789
CaiI CAGNNNCTG 1 cut(s) 379
CciI TCATGA 1 cut(s) 559
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 3 cut(s) 298, 688, 780
Cfr9I CCCGGG 1 cut(s) 384
ClaI ATCGAT 2 cut(s) 91, 175
CseI GACGC 1 cut(s) 631
Csp6I GTAC 2 cut(s) 118, 496
CviAII CATG 4 cut(s) 500, 560, 767, 990
CviJI RGCY 9 cut(s) 107, 152, 218, 319, 342, 372, 443, 545, 781
CviKI_1 RGCY 9 cut(s) 107, 152, 218, 319, 342, 372, 443, 545, 781
CviQI GTAC 2 cut(s) 118, 496
DdeI CTNAG 1 cut(s) 615
DinI GGCGCC 1 cut(s) 25
DpnI GATC 6 cut(s) 328, 465, 549, 596, 869, 930
DpnII GATC 6 cut(s) 326, 463, 547, 594, 867, 928
DraI TTTAAA 1 cut(s) 571
EaeI YGGCCR 2 cut(s) 105, 216
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 2 cut(s) 836, 905
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 807
Eco47I GGWCC 2 cut(s) 298, 688
Eco53kI GAGCTC 1 cut(s) 319
Eco88I CYCGRG 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 319
EcoRII CCWGG 3 cut(s) 607, 721, 947
EcoT14I CCWWGG 2 cut(s) 836, 905
EcoT38I GRGCYC 1 cut(s) 321
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 2 cut(s) 836, 905
Esp3I CGTCTC 1 cut(s) 614
FaeI CATG 4 cut(s) 503, 563, 770, 993
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FaqI GGGAC 1 cut(s) 367
FatI CATG 4 cut(s) 499, 559, 766, 989
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 2 cut(s) 108, 474
FokI GGATG 2 cut(s) 428, 921
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 2 cut(s) 108, 474
FspBI CTAG 2 cut(s) 74, 806
GlaI GCGC 1 cut(s) 25
GluI GCNGC 2 cut(s) 108, 474
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 3 cut(s) 107, 218, 781
HapII CCGG 3 cut(s) 301, 322, 385
HgaI GACGC 1 cut(s) 631
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 4 cut(s) 503, 563, 770, 993
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HpaI GTTAAC 1 cut(s) 31
HpaII CCGG 3 cut(s) 301, 322, 385
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 8 cut(s) 204, 285, 316, 447, 493, 790, 822, 919
Hpy188III TCNNGA 7 cut(s) 322, 517, 560, 586, 639, 734, 943
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 2 cut(s) 57, 256
HpyCH4III ACNGT 4 cut(s) 131, 229, 338, 487
HpyCH4V TGCA 4 cut(s) 680, 798, 845, 912
HpyF3I CTNAG 1 cut(s) 615
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 4 cut(s) 503, 563, 770, 993
HspAI GCGC 1 cut(s) 24
KasI GGCGCC 1 cut(s) 23
Kpn2I TCCGGA 1 cut(s) 321
KspAI GTTAAC 1 cut(s) 31
Kzo9I GATC 6 cut(s) 326, 463, 547, 594, 867, 928
LmnI GCTCC 3 cut(s) 316, 324, 809
MaeI CTAG 2 cut(s) 74, 806
MaeIII GTNAC 5 cut(s) 467, 647, 661, 943, 994
MalI GATC 6 cut(s) 328, 465, 549, 596, 869, 930
MboI GATC 6 cut(s) 326, 463, 547, 594, 867, 928
MboII GAAGA 3 cut(s) 77, 505, 542
MflI RGATCY 2 cut(s) 326, 594
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 5 cut(s) 156, 220, 393, 696, 980
Mly113I GGCGCC 1 cut(s) 24
MlyI GAGTC 4 cut(s) 79, 361, 370, 782
MnlI CCTC 2 cut(s) 412, 950
MroI TCCGGA 1 cut(s) 321
MseI TTAA 4 cut(s) 30, 357, 570, 627
MslI CAYNNNNRTG 2 cut(s) 504, 850
MspI CCGG 3 cut(s) 301, 322, 385
MspR9I CCNGG 6 cut(s) 302, 385, 386, 609, 723, 949
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 3 cut(s) 609, 723, 949
MvnI CGCG 2 cut(s) 182, 473
NarI GGCGCC 1 cut(s) 24
NciI CCSGG 3 cut(s) 302, 385, 386
NdeII GATC 6 cut(s) 326, 463, 547, 594, 867, 928
NlaIII CATG 4 cut(s) 503, 563, 770, 993
NlaIV GGNNCC 2 cut(s) 25, 596
NmeAIII GCCGAG 1 cut(s) 649
NmuCI GTSAC 2 cut(s) 661, 943
PagI TCATGA 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 88
PctI GAATGC 1 cut(s) 682
PfeI GAWTC 7 cut(s) 177, 199, 205, 280, 311, 739, 920
PflFI GACNNNGTC 1 cut(s) 379
PfoI TCCNGGA 2 cut(s) 300, 721
PkrI GCNGC 2 cut(s) 109, 475
PleI GAGTC 4 cut(s) 78, 360, 370, 781
PluTI GGCGCC 1 cut(s) 27
PpsI GAGTC 4 cut(s) 78, 360, 370, 781
Psp124BI GAGCTC 1 cut(s) 321
Psp6I CCWGG 3 cut(s) 607, 721, 947
PspGI CCWGG 3 cut(s) 607, 721, 947
PspN4I GGNNCC 2 cut(s) 25, 596
PspPI GGNCC 3 cut(s) 298, 688, 780
PstNI CAGNNNCTG 1 cut(s) 379
PsuI RGATCY 2 cut(s) 326, 594
PsyI GACNNNGTC 1 cut(s) 379
RsaI GTAC 2 cut(s) 119, 497
RsaNI GTAC 2 cut(s) 118, 496
RseI CAYNNNNRTG 2 cut(s) 504, 850
SacI GAGCTC 1 cut(s) 321
SaqAI TTAA 4 cut(s) 30, 357, 570, 627
SatI GCNGC 2 cut(s) 108, 474
Sau3AI GATC 6 cut(s) 326, 463, 547, 594, 867, 928
Sau96I GGNCC 3 cut(s) 298, 688, 780
SchI GAGTC 4 cut(s) 79, 361, 370, 782
ScrFI CCNGG 6 cut(s) 302, 385, 386, 609, 723, 949
SduI GDGCHC 1 cut(s) 321
SfoI GGCGCC 1 cut(s) 25
SinI GGWCC 2 cut(s) 298, 688
SmaI CCCGGG 1 cut(s) 386
SmiMI CAYNNNNRTG 2 cut(s) 504, 850
Sse9I AATT 5 cut(s) 156, 220, 393, 696, 980
SsiI CCGC 5 cut(s) 108, 161, 182, 292, 473
SspDI GGCGCC 1 cut(s) 23
SspMI CTAG 2 cut(s) 74, 806
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 6 cut(s) 300, 383, 384, 607, 721, 947
StyI CCWWGG 2 cut(s) 836, 905
TaaI ACNGT 4 cut(s) 131, 229, 338, 487
TaqI TCGA 3 cut(s) 91, 175, 208
TasI AATT 5 cut(s) 156, 220, 393, 696, 980
TatI WGTACW 1 cut(s) 117
TauI GCSGC 2 cut(s) 110, 476
TfiI GAWTC 7 cut(s) 177, 199, 205, 280, 311, 739, 920
Tru1I TTAA 4 cut(s) 30, 357, 570, 627
Tru9I TTAA 4 cut(s) 30, 357, 570, 627
TscAI CASTG 1 cut(s) 975
TseFI GTSAC 2 cut(s) 661, 943
Tsp45I GTSAC 2 cut(s) 661, 943
TspDTI ATGAA 5 cut(s) 77, 548, 555, 944, 990
TspMI CCCGGG 1 cut(s) 384
TspRI CASTG 1 cut(s) 975
Tth111I GACNNNGTC 1 cut(s) 379
VpaK11BI GGWCC 2 cut(s) 298, 688
XmaI CCCGGG 1 cut(s) 384
XspI CTAG 2 cut(s) 74, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.