Prupe.7G189700_v2.0.a1

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0012369
Physical Location & Seq
Forward (+)
18179876 .. 18183621
3746 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G189700.3

Sequence Viewer

Length: 1080 bp
ATGTTGTTTTTGAAAAAGCAAGGCGGCTTGCGCTTCTTCACAAACTTAAAGCACCAAACTTTACTTCATCGTCATCAGTTTACAGAGGCTATGGCTGCTCATTTGTATAACCATGGTAGCTACAAAGGGTTTCAAGGCCGAGCTCAATGGCCGCAATCCCATTTCTCCAGAGACCACTCTTGCAAGTATGCAACTTTCCCTGGGAAAATCAGGAATACGAGGAACACACGTCTTGGCCGCAATTTTTTCGTAAAAGGGTGCTTCAAATCACAGCATCACCACCATCAGTCTCATAGCAGTAGCATAAAGCGTCACAGCAATGCCTTTGAGAATCCCAATGCTCAATTGGTCCGTGATCTTGAATTGGACCTCCGCAGCTCTGATGCCATTGGATTGGCCTCCTCTAATGGTTGCCAGGAGCGTGGAAGCCATACTGGAATCCCTCCCCATTCTTTGCCCGATGATAAAGTCGTCGTTGCTGTTGATGTGGATGAGGTGTTGGGAAACTTTGTGTCGGCTCTCAACAGGTTTATTGCTGATCGTTACTCCTCCCACCATTCAGTTTCAGAGTACCATGTCTATGAGTTCTTCAAGATATGGAACTGTTCTCGTGATGAAGCCGATGTTCGTGTGCATGAGTTCTTTAAAACATCATATTTCAAGACCGGGATCCACCCACTCCCAGGTGCTCAGGAGACAATTCATAAGTTGTCAGGATTTTGTGACCTGTCAGTTGTGACGTCTAGACAGAATGCAATTAAGGACCACACAATTCAATGGATTGAAAAACATTTTCCAGGACTGTTTCAGGAGATTCACTTTGGCAACCACTTTGCTTTAGATGGAGAGTCTAGGCCTAAGTCAGAAATATGCAGGTCCTTGGGAGCAAAGGTTCTAATTGATGACAATCCAAGATATGCGCTTGAATGTGCTGAGGCTGGGATCAGGGTTCTTCTTTTTGATTATGAGAACTCATATCCGTGGTGCAAGACTGAGTCAATTATTCAGCACCCCCTGGTCACCAAGGTCCATAACTGGGAAGCAGTGGAGAAGCACTTAATGACATGGATTATTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

360

Amino Acids

41.29

Weight (kDa)

8.69

Isoelectric Point (pI)

28.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33140
fragaria_vesca FvH4_1g09650 FvH4_1g09650
malus_domestica MD02G1105400.v1.1 MD15G1225300.v1.1
prunus_persica Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1
pyrus_communis pycom15g20010
rosa_chinensis RchiOBHm_Chr2g0096491
rosa_laevigata RLG00000016644
rosa_multiflora Rmu_sc0004096.1_g000012
rosa_roxburghii Rroxscaffold_2G00145600
rosa_rugosa Rorug02G0058100
rosa_samantha Rh2AG105100 Rh2BG107200 Rh2CG109000 Rh2DG108200
rosa_wichuraiana Rw2G008120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 743
Acc36I ACCTGC 1 cut(s) 864
AciI CCGC 4 cut(s) 24, 152, 238, 373
AclWI GGATC 3 cut(s) 664, 677, 950
AcoI YGGCCR 2 cut(s) 149, 235
AcyI GRCGYC 1 cut(s) 740
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 2 cut(s) 683, 1036
AflIII ACRYGT 1 cut(s) 227
AgsI TTSAA 9 cut(s) 13, 134, 265, 362, 592, 661, 776, 785, 926
AjiI CACGTC 1 cut(s) 230
AjnI CCWGG 5 cut(s) 199, 414, 682, 796, 1014
AjuI GAANNNNNNNTTGG 2 cut(s) 48, 80
AloI GAACNNNNNNTCC 2 cut(s) 876, 908
AluBI AGCT 3 cut(s) 120, 143, 378
AluI AGCT 3 cut(s) 120, 143, 378
Alw21I GWGCWC 2 cut(s) 145, 691
Alw26I GTCTC 3 cut(s) 165, 294, 689
AlwI GGATC 3 cut(s) 664, 677, 950
AoxI GGCC 5 cut(s) 136, 149, 235, 396, 854
ApeKI GCWGC 2 cut(s) 95, 375
AspLEI GCGC 2 cut(s) 33, 922
AspS9I GGNCC 5 cut(s) 349, 367, 763, 876, 1027
AsuC2I CCSGG 1 cut(s) 667
AsuHPI GGTGA 2 cut(s) 269, 1012
AvaII GGWCC 5 cut(s) 349, 367, 763, 876, 1027
BamHI GGATCC 1 cut(s) 669
BanII GRGCYC 1 cut(s) 145
BauI CACGAG 1 cut(s) 609
Bbv12I GWGCWC 2 cut(s) 145, 691
BbvCI CCTCAGC 1 cut(s) 933
BbvI GCAGC 2 cut(s) 82, 387
BccI CCATC 2 cut(s) 291, 836
BcgI CGANNNNNNTGC 2 cut(s) 229, 263
BciT130I CCWGG 5 cut(s) 201, 416, 684, 798, 1016
BcnI CCSGG 1 cut(s) 667
BcoDI GTCTC 3 cut(s) 165, 294, 689
BfaI CTAG 2 cut(s) 744, 852
BfuAI ACCTGC 1 cut(s) 864
BisI GCNGC 5 cut(s) 25, 96, 152, 238, 376
BlsI GCNGC 5 cut(s) 26, 97, 153, 239, 377
Bme1390I CCNGG 6 cut(s) 201, 416, 667, 684, 798, 1016
Bme18I GGWCC 5 cut(s) 349, 367, 763, 876, 1027
BmgBI CACGTC 1 cut(s) 230
BmgT120I GGNCC 5 cut(s) 349, 367, 763, 876, 1027
BmiI GGNNCC 1 cut(s) 671
BmrFI CCNGG 6 cut(s) 201, 416, 667, 684, 798, 1016
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 2 cut(s) 283, 373
BmuI ACTGGG 1 cut(s) 1045
BpmI CTGGAG 1 cut(s) 151
Bpu10I CCTNAGC 2 cut(s) 690, 933
BpuMI CCSGG 1 cut(s) 667
BsaBI GATNNNNATC 1 cut(s) 906
BsaHI GRCGYC 1 cut(s) 740
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 8 cut(s) 112, 199, 200, 682, 879, 980, 1014, 1023
BsaXI ACNNNNNCTCC 2 cut(s) 876, 906
Bsc4I CCNNNNNNNGG 2 cut(s) 683, 1036
Bse1I ACTGG 2 cut(s) 439, 1040
Bse3DI GCAATG 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 906
BseBI CCWGG 5 cut(s) 201, 416, 684, 798, 1016
BseDI CCNNGG 8 cut(s) 112, 199, 200, 682, 879, 980, 1014, 1023
BseGI GGATG 1 cut(s) 496
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 2 cut(s) 683, 1036
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 3 cut(s) 704, 924, 984
BseNI ACTGG 2 cut(s) 439, 1040
BseRI GAGGAG 2 cut(s) 391, 538
BseXI GCAGC 2 cut(s) 82, 387
BseYI CCCAGC 1 cut(s) 938
BshFI GGCC 5 cut(s) 138, 151, 237, 398, 856
BsiHKAI GWGCWC 2 cut(s) 145, 691
BsiSI CCGG 1 cut(s) 666
BslI CCNNNNNNNGG 2 cut(s) 683, 1036
BsmAI GTCTC 3 cut(s) 165, 294, 689
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 5 cut(s) 138, 151, 237, 398, 856
Bso31I GGTCTC 1 cut(s) 165
Bsp1286I GDGCHC 2 cut(s) 145, 691
Bsp143I GATC 4 cut(s) 355, 538, 669, 942
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 4 cut(s) 24, 152, 238, 373
BspANI GGCC 5 cut(s) 138, 151, 237, 398, 856
BspCNI CTCAG 3 cut(s) 703, 925, 985
BspLI GGNNCC 1 cut(s) 671
BspMI ACCTGC 1 cut(s) 864
BspPI GGATC 3 cut(s) 664, 677, 950
BspTNI GGTCTC 1 cut(s) 165
BsrDI GCAATG 1 cut(s) 325
BsrI ACTGG 2 cut(s) 439, 1040
BssECI CCNNGG 8 cut(s) 112, 199, 200, 682, 879, 980, 1014, 1023
BssMI GATC 4 cut(s) 355, 538, 669, 942
BssNI GRCGYC 1 cut(s) 740
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 3 cut(s) 112, 879, 1023
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 5 cut(s) 201, 416, 684, 798, 1016
Bst4CI ACNGT 2 cut(s) 605, 804
BstACI GRCGYC 1 cut(s) 740
BstC8I GCNNGC 1 cut(s) 29
BstDEI CTNAG 4 cut(s) 690, 858, 933, 993
BstDSI CCRYGG 2 cut(s) 112, 980
BstEII GGTNACC 1 cut(s) 1018
BstF5I GGATG 1 cut(s) 496
BstHHI GCGC 2 cut(s) 33, 922
BstKTI GATC 4 cut(s) 358, 541, 672, 945
BstMAI GTCTC 3 cut(s) 165, 294, 689
BstMBI GATC 4 cut(s) 355, 538, 669, 942
BstMWI GCNNNNNNNGC 2 cut(s) 30, 95
BstNI CCWGG 5 cut(s) 201, 416, 684, 798, 1016
BstPI GGTNACC 1 cut(s) 1018
BstSCI CCNGG 6 cut(s) 199, 414, 665, 682, 796, 1014
BstV1I GCAGC 2 cut(s) 82, 387
BstX2I RGATCY 1 cut(s) 669
BstXI CCANNNNNNTGG 2 cut(s) 394, 422
BstYI RGATCY 1 cut(s) 669
BsuRI GGCC 5 cut(s) 138, 151, 237, 398, 856
BtgI CCRYGG 2 cut(s) 112, 980
BtrI CACGTC 1 cut(s) 230
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 1050
BtsIMutI CAGTG 1 cut(s) 1050
BveI ACCTGC 1 cut(s) 864
Cac8I GCNNGC 1 cut(s) 29
CfoI GCGC 2 cut(s) 33, 922
Cfr13I GGNCC 5 cut(s) 349, 367, 763, 876, 1027
CseI GACGC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 571
CviAII CATG 4 cut(s) 113, 575, 635, 1065
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 4 cut(s) 690, 858, 933, 993
DpnI GATC 4 cut(s) 357, 540, 671, 944
DpnII GATC 4 cut(s) 355, 538, 669, 942
DraI TTTAAA 1 cut(s) 646
EaeI YGGCCR 2 cut(s) 149, 235
Ecl136II GAGCTC 1 cut(s) 143
Eco130I CCWWGG 3 cut(s) 112, 879, 1023
Eco147I AGGCCT 1 cut(s) 856
Eco24I GRGCYC 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 165
Eco47I GGWCC 5 cut(s) 349, 367, 763, 876, 1027
Eco53kI GAGCTC 1 cut(s) 143
Eco91I GGTNACC 1 cut(s) 1018
EcoICRI GAGCTC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 876
EcoO65I GGTNACC 1 cut(s) 1018
EcoRII CCWGG 5 cut(s) 199, 414, 682, 796, 1014
EcoT14I CCWWGG 3 cut(s) 112, 879, 1023
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 3 cut(s) 112, 879, 1023
FaeI CATG 4 cut(s) 116, 578, 638, 1068
FatI CATG 4 cut(s) 112, 574, 634, 1064
Fnu4HI GCNGC 5 cut(s) 25, 96, 152, 238, 376
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 5 cut(s) 25, 96, 152, 238, 376
FspBI CTAG 2 cut(s) 744, 852
GlaI GCGC 2 cut(s) 32, 921
GluI GCNGC 5 cut(s) 25, 96, 152, 238, 376
GsaI CCCAGC 1 cut(s) 942
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 5 cut(s) 138, 151, 237, 398, 856
HapII CCGG 1 cut(s) 666
HgaI GACGC 1 cut(s) 299
HhaI GCGC 2 cut(s) 33, 922
Hin1I GRCGYC 1 cut(s) 740
Hin1II CATG 4 cut(s) 116, 578, 638, 1068
Hin6I GCGC 2 cut(s) 31, 920
HinP1I GCGC 2 cut(s) 31, 920
HinfI GANTC 5 cut(s) 331, 438, 814, 848, 995
HpaII CCGG 1 cut(s) 666
HphI GGTGA 2 cut(s) 269, 1012
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 3 cut(s) 382, 568, 865
Hpy8I GTNNAC 1 cut(s) 81
Hpy99I CGWCG 1 cut(s) 476
HpyCH4III ACNGT 2 cut(s) 605, 804
HpyCH4IV ACGT 2 cut(s) 229, 740
HpyCH4V TGCA 6 cut(s) 183, 191, 634, 755, 873, 987
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 95
HpyF3I CTNAG 4 cut(s) 690, 858, 933, 993
HpySE526I ACGT 2 cut(s) 229, 740
Hsp92I GRCGYC 1 cut(s) 740
Hsp92II CATG 4 cut(s) 116, 578, 638, 1068
HspAI GCGC 2 cut(s) 31, 920
Kzo9I GATC 4 cut(s) 355, 538, 669, 942
LmnI GCTCC 2 cut(s) 418, 884
Lsp1109I GCAGC 2 cut(s) 82, 387
LweI GCATC 2 cut(s) 283, 373
MaeI CTAG 2 cut(s) 744, 852
MaeII ACGT 2 cut(s) 229, 740
MaeIII GTNAC 5 cut(s) 311, 542, 722, 736, 1018
MalI GATC 4 cut(s) 357, 540, 671, 944
MboI GATC 4 cut(s) 355, 538, 669, 942
MboII GAAGA 3 cut(s) 28, 580, 944
MfeI CAATTG 1 cut(s) 344
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 2 cut(s) 145, 691
MluCI AATT 8 cut(s) 241, 344, 362, 699, 756, 771, 897, 999
MlyI GAGTC 2 cut(s) 857, 1004
MnlI CCTC 9 cut(s) 79, 213, 380, 409, 412, 453, 487, 559, 928
MseI TTAA 4 cut(s) 47, 645, 759, 1058
MslI CAYNNNNRTG 3 cut(s) 318, 579, 979
MspI CCGG 1 cut(s) 666
MspR9I CCNGG 6 cut(s) 201, 416, 667, 684, 798, 1016
MunI CAATTG 1 cut(s) 344
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 5 cut(s) 201, 416, 684, 798, 1016
MwoI GCNNNNNNNGC 2 cut(s) 30, 95
NciI CCSGG 1 cut(s) 667
NcoI CCATGG 1 cut(s) 112
NdeII GATC 4 cut(s) 355, 538, 669, 942
NlaIII CATG 4 cut(s) 116, 578, 638, 1068
NlaIV GGNNCC 1 cut(s) 671
NmeAIII GCCGAG 1 cut(s) 164
NmuCI GTSAC 4 cut(s) 311, 722, 736, 1018
PasI CCCWGGG 1 cut(s) 200
PceI AGGCCT 1 cut(s) 856
PctI GAATGC 1 cut(s) 757
PfeI GAWTC 3 cut(s) 331, 438, 814
PflFI GACNNNGTC 1 cut(s) 994
PfoI TCCNGGA 1 cut(s) 796
PkrI GCNGC 5 cut(s) 26, 97, 153, 239, 377
PleI GAGTC 2 cut(s) 856, 1003
PpsI GAGTC 2 cut(s) 856, 1003
PpuMI RGGWCCY 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 145
Psp5II RGGWCCY 1 cut(s) 876
Psp6I CCWGG 5 cut(s) 199, 414, 682, 796, 1014
PspEI GGTNACC 1 cut(s) 1018
PspFI CCCAGC 1 cut(s) 938
PspGI CCWGG 5 cut(s) 199, 414, 682, 796, 1014
PspN4I GGNNCC 1 cut(s) 671
PspPI GGNCC 5 cut(s) 349, 367, 763, 876, 1027
PspPPI RGGWCCY 1 cut(s) 876
PsuI RGATCY 1 cut(s) 669
PsyI GACNNNGTC 1 cut(s) 994
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 3 cut(s) 318, 579, 979
SacI GAGCTC 1 cut(s) 145
SaqAI TTAA 4 cut(s) 47, 645, 759, 1058
SatI GCNGC 5 cut(s) 25, 96, 152, 238, 376
Sau3AI GATC 4 cut(s) 355, 538, 669, 942
Sau96I GGNCC 5 cut(s) 349, 367, 763, 876, 1027
SchI GAGTC 2 cut(s) 857, 1004
ScrFI CCNGG 6 cut(s) 201, 416, 667, 684, 798, 1016
SduI GDGCHC 2 cut(s) 145, 691
SfaNI GCATC 2 cut(s) 283, 373
SinI GGWCC 5 cut(s) 349, 367, 763, 876, 1027
SmiMI CAYNNNNRTG 3 cut(s) 318, 579, 979
Sse9I AATT 8 cut(s) 241, 344, 362, 699, 756, 771, 897, 999
SseBI AGGCCT 1 cut(s) 856
SsiI CCGC 4 cut(s) 24, 152, 238, 373
SspMI CTAG 2 cut(s) 744, 852
SstI GAGCTC 1 cut(s) 145
StuI AGGCCT 1 cut(s) 856
StyD4I CCNGG 6 cut(s) 199, 414, 665, 682, 796, 1014
StyI CCWWGG 3 cut(s) 112, 879, 1023
TaaI ACNGT 2 cut(s) 605, 804
TaiI ACGT 2 cut(s) 232, 743
TasI AATT 8 cut(s) 241, 344, 362, 699, 756, 771, 897, 999
TauI GCSGC 3 cut(s) 27, 154, 240
TfiI GAWTC 3 cut(s) 331, 438, 814
Tru1I TTAA 4 cut(s) 47, 645, 759, 1058
Tru9I TTAA 4 cut(s) 47, 645, 759, 1058
TscAI CASTG 1 cut(s) 1050
TseFI GTSAC 4 cut(s) 311, 722, 736, 1018
TseI GCWGC 2 cut(s) 95, 375
Tsp45I GTSAC 4 cut(s) 311, 722, 736, 1018
TspDTI ATGAA 4 cut(s) 56, 630, 692, 1065
TspGWI ACGGA 2 cut(s) 341, 969
TspRI CASTG 1 cut(s) 1050
Tth111I GACNNNGTC 1 cut(s) 994
VpaK11BI GGWCC 5 cut(s) 349, 367, 763, 876, 1027
XbaI TCTAGA 1 cut(s) 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XspI CTAG 2 cut(s) 744, 852
ZraI GACGTC 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.