Rroxscaffold_2G00145600

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012369
Physical Location & Seq
Forward (+)
83464595 .. 83467668
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145600.1

Sequence Viewer

Length: 1167 bp
ATGTTGGTGTTGAAAAGGCAAGGCGCCGTTAACTTTCTCACACACCCTTCAAAGCACCACCAACCTTTGAGTTTAGGTCGTCGTCTTCATCGATTTACAGAGATGGCCGCCTATTTGTACAACCATAACTGCAAGGGATTTCATGCACAAGCCCCAATTGCGCACCCAAATCATCGATTGGCGGGTCTTGTTCGCCAGATTCGGAATCGAAAGACGGCCAATTTGACCGTGAAATGTTGCTTCAATAGTAGCAATCATAACAATAGAGTGAGGGGCAATGTGTTTGAGAATCCGAATGGGCAGTTGGTCCGGGATTTGGATTTGGAGCTCCGGAGATCCCAGAATGGTGGCTTTCAGGGGAGTCATAATGGGTTTCACAGCCAGACTTTGTCCCGGGATAAAATTGTGGTTGCTGTTGATGTGGATGAGGTTTTGGGCAACTTTGTGTCGGCTCTGAACAGGTTTATTGCTGATCGTTACGCGGCGAACCATACGGTTTCGGAGTACCATGTGTATGAGTTCTTCAAGGGGTTTGATTTGTTACTCCAGCTATCTGAGTTCTTTGTTCTGTGGTTAATGGTGCCAGTATGTTCATTGGCAACAATCTTTTCGAACATATATGGAAGTGTTCCCGTGATGAAGGTTGTATTTGGATATGAAACCTTTGAATCTCTAGATTCTCTTGATGTAAAGGTCAAATCTTTAGCCGATCTTCGTGTTCATGAGTTCTTTAAAACATCATATTTCAAGACTGGGATCCACCCCCTCCCAGGTGCTAAGGAGACGCTTAATAAGTTGTCAGGATTTTGTAACCTGTCAGTTGTGACTTCTCGGCAGAATGCAATCAAGGACCACACAATTCAATGGATTGAAAGTCATTTTCCAGGACTATTTCAGGAGATTCACTTCGGCAACCACTTTGCTCTACATGGAGAGTCAAGGCCTAAGTCAGAAATATGCAGGTCTCTAGGAGCAAAGGTTCTGATTGATGACAATCCAAGGTATGCACTTGAATGTGCCGAAGTTGGGATCAGGGTTCTACTTTTTGATTACGAGAACTCATATCCGTGGTGCAAGTCCGATTCTGTTGATCTACACCCCCTCGTGACCAGGGTTCATAACTGGGAAGCAGTGGAGAAGCAATTATTGGCATGGGTTACTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

388

Amino Acids

44.23

Weight (kDa)

8.82

Isoelectric Point (pI)

30.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT5C PF06941 238 - 380 1.3e-08 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33140
fragaria_vesca FvH4_1g09650 FvH4_1g09650
malus_domestica MD02G1105400.v1.1 MD15G1225300.v1.1
prunus_persica Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1
pyrus_communis pycom15g20010
rosa_chinensis RchiOBHm_Chr2g0096491
rosa_laevigata RLG00000016644
rosa_multiflora Rmu_sc0004096.1_g000012
rosa_roxburghii Rroxscaffold_2G00145600
rosa_rugosa Rorug02G0058100
rosa_samantha Rh2AG105100 Rh2BG107200 Rh2CG109000 Rh2DG108200
rosa_wichuraiana Rw2G008120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 951
AccB1I GGYRCC 2 cut(s) 23, 580
AccII CGCG 1 cut(s) 482
AccIII TCCGGA 1 cut(s) 330
AciI CCGC 3 cut(s) 108, 182, 482
AclWI GGATC 4 cut(s) 330, 751, 764, 1037
AcoI YGGCCR 2 cut(s) 105, 216
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 2 cut(s) 119, 506
AfiI CCNNNNNNNGG 3 cut(s) 316, 770, 1026
AgsI TTSAA 9 cut(s) 13, 51, 244, 526, 668, 748, 863, 872, 1013
AjnI CCWGG 3 cut(s) 769, 883, 1109
AjuI GAANNNNNNNTTGG 4 cut(s) 287, 319, 1130, 1162
AloI GAACNNNNNNTCC 2 cut(s) 963, 995
AluBI AGCT 2 cut(s) 328, 550
AluI AGCT 2 cut(s) 328, 550
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 2 cut(s) 776, 969
AlwI GGATC 4 cut(s) 330, 751, 764, 1037
Ama87I CYCGRG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 330
AoxI GGCC 3 cut(s) 105, 216, 941
ArsI GACNNNNNNTTYG 2 cut(s) 205, 237
AspLEI GCGC 2 cut(s) 26, 163
AspS9I GGNCC 2 cut(s) 307, 850
AsuC2I CCSGG 3 cut(s) 311, 394, 395
AsuII TTCGAA 1 cut(s) 611
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 2 cut(s) 307, 850
BamHI GGATCC 1 cut(s) 756
BanI GGYRCC 2 cut(s) 23, 580
BanII GRGCYC 1 cut(s) 330
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BauI CACGAG 1 cut(s) 1103
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 330
BccI CCATC 1 cut(s) 97
BceAI ACGGC 2 cut(s) 11, 231
BciT130I CCWGG 3 cut(s) 771, 885, 1111
BcnI CCSGG 3 cut(s) 311, 394, 395
BcoDI GTCTC 2 cut(s) 776, 969
BfaI CTAG 2 cut(s) 674, 968
BfoI RGCGCY 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 951
BisI GCNGC 2 cut(s) 108, 483
BlsI GCNGC 2 cut(s) 109, 484
Bme1390I CCNGG 6 cut(s) 311, 394, 395, 771, 885, 1111
Bme18I GGWCC 2 cut(s) 307, 850
BmeT110I CYCGRG 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 307, 850
BmiI GGNNCC 3 cut(s) 25, 582, 758
BmrFI CCNGG 6 cut(s) 311, 394, 395, 771, 885, 1111
BmrI ACTGGG 2 cut(s) 762, 1132
BmuI ACTGGG 2 cut(s) 762, 1132
BpiI GAAGAC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 530
Bpu10I CCTNAGC 1 cut(s) 777
Bpu14I TTCGAA 1 cut(s) 611
BpuMI CCSGG 3 cut(s) 311, 394, 395
Bsa29I ATCGAT 2 cut(s) 91, 175
BsaBI GATNNNNATC 1 cut(s) 993
BsaHI GRCGYC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 969
BsaJI CCNNGG 5 cut(s) 393, 769, 998, 1067, 1110
BsaWI WCCGGW 1 cut(s) 330
BsaXI ACNNNNNCTCC 2 cut(s) 963, 993
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 770, 1026
Bse1I ACTGG 3 cut(s) 584, 757, 1127
Bse3DI GCAATG 1 cut(s) 283
Bse8I GATNNNNATC 1 cut(s) 993
BseAI TCCGGA 1 cut(s) 330
BseBI CCWGG 3 cut(s) 771, 885, 1111
BseCI ATCGAT 2 cut(s) 91, 175
BseDI CCNNGG 5 cut(s) 393, 769, 998, 1067, 1110
BseGI GGATG 1 cut(s) 430
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 3 cut(s) 316, 770, 1026
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 3 cut(s) 584, 757, 1127
Bsh1236I CGCG 1 cut(s) 482
BshFI GGCC 3 cut(s) 107, 218, 943
BshNI GGYRCC 2 cut(s) 23, 580
BshVI ATCGAT 2 cut(s) 91, 175
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 3 cut(s) 310, 331, 394
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 3 cut(s) 316, 770, 1026
BsmAI GTCTC 2 cut(s) 776, 969
BsmBI CGTCTC 1 cut(s) 776
BsmFI GGGAC 1 cut(s) 376
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 3 cut(s) 107, 218, 943
Bso31I GGTCTC 1 cut(s) 969
BsoBI CYCGRG 1 cut(s) 393
Bsp119I TTCGAA 1 cut(s) 611
Bsp1286I GDGCHC 1 cut(s) 330
Bsp13I TCCGGA 1 cut(s) 330
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 6 cut(s) 335, 472, 709, 756, 1029, 1090
BspACI CCGC 3 cut(s) 108, 182, 482
BspANI GGCC 3 cut(s) 107, 218, 943
BspCNI CTCAG 1 cut(s) 547
BspDI ATCGAT 2 cut(s) 91, 175
BspEI TCCGGA 1 cut(s) 330
BspFNI CGCG 1 cut(s) 482
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 3 cut(s) 25, 582, 758
BspMI ACCTGC 1 cut(s) 951
BspPI GGATC 4 cut(s) 330, 751, 764, 1037
BspT104I TTCGAA 1 cut(s) 611
BspT107I GGYRCC 2 cut(s) 23, 580
BspTNI GGTCTC 1 cut(s) 969
BsrDI GCAATG 1 cut(s) 283
BsrGI TGTACA 1 cut(s) 117
BsrI ACTGG 3 cut(s) 584, 757, 1127
BssECI CCNNGG 5 cut(s) 393, 769, 998, 1067, 1110
BssMI GATC 6 cut(s) 335, 472, 709, 756, 1029, 1090
BssNI GRCGYC 1 cut(s) 24
BssSI CACGAG 1 cut(s) 1103
BssT1I CCWWGG 1 cut(s) 998
Bst2BI CACGAG 1 cut(s) 1103
Bst2UI CCWGG 3 cut(s) 771, 885, 1111
Bst4CI ACNGT 2 cut(s) 229, 496
BstACI GRCGYC 1 cut(s) 24
BstAUI TGTACA 1 cut(s) 117
BstBI TTCGAA 1 cut(s) 611
BstDEI CTNAG 3 cut(s) 555, 777, 945
BstDSI CCRYGG 1 cut(s) 1067
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 1 cut(s) 482
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 2 cut(s) 26, 163
BstKTI GATC 6 cut(s) 338, 475, 712, 759, 1032, 1093
BstMAI GTCTC 2 cut(s) 776, 969
BstMBI GATC 6 cut(s) 335, 472, 709, 756, 1029, 1090
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNI CCWGG 3 cut(s) 771, 885, 1111
BstSCI CCNGG 6 cut(s) 309, 392, 393, 769, 883, 1109
BstUI CGCG 1 cut(s) 482
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 2 cut(s) 335, 756
BstXI CCANNNNNNTGG 1 cut(s) 347
BstYI RGATCY 2 cut(s) 335, 756
Bsu15I ATCGAT 2 cut(s) 91, 175
BsuRI GGCC 3 cut(s) 107, 218, 943
BsuTUI ATCGAT 2 cut(s) 91, 175
BtgI CCRYGG 1 cut(s) 1067
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 1 cut(s) 1137
BveI ACCTGC 1 cut(s) 951
CciI TCATGA 1 cut(s) 721
CfoI GCGC 2 cut(s) 26, 163
Cfr13I GGNCC 2 cut(s) 307, 850
Cfr9I CCCGGG 1 cut(s) 393
ClaI ATCGAT 2 cut(s) 91, 175
CseI GACGC 1 cut(s) 793
Csp6I GTAC 2 cut(s) 118, 505
CviAII CATG 5 cut(s) 143, 509, 722, 929, 1152
CviQI GTAC 2 cut(s) 118, 505
DdeI CTNAG 3 cut(s) 555, 777, 945
DinI GGCGCC 1 cut(s) 25
DpnI GATC 6 cut(s) 337, 474, 711, 758, 1031, 1092
DpnII GATC 6 cut(s) 335, 472, 709, 756, 1029, 1090
DraI TTTAAA 1 cut(s) 733
EaeI YGGCCR 2 cut(s) 105, 216
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 998
Eco147I AGGCCT 1 cut(s) 943
Eco24I GRGCYC 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 969
Eco47I GGWCC 2 cut(s) 307, 850
Eco53kI GAGCTC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 328
EcoRII CCWGG 3 cut(s) 769, 883, 1109
EcoT14I CCWWGG 1 cut(s) 998
EcoT38I GRGCYC 1 cut(s) 330
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 998
Esp3I CGTCTC 1 cut(s) 776
FaeI CATG 5 cut(s) 146, 512, 725, 932, 1155
FaqI GGGAC 1 cut(s) 376
FatI CATG 5 cut(s) 142, 508, 721, 928, 1151
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 2 cut(s) 108, 483
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 2 cut(s) 108, 483
FspBI CTAG 2 cut(s) 674, 968
FspI TGCGCA 1 cut(s) 162
GlaI GCGC 2 cut(s) 25, 162
GluI GCNGC 2 cut(s) 108, 483
GsuI CTGGAG 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 3 cut(s) 107, 218, 943
HapII CCGG 3 cut(s) 310, 331, 394
HgaI GACGC 1 cut(s) 793
HhaI GCGC 2 cut(s) 26, 163
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 5 cut(s) 146, 512, 725, 932, 1155
Hin6I GCGC 2 cut(s) 24, 161
HinP1I GCGC 2 cut(s) 24, 161
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 9 cut(s) 199, 205, 289, 361, 668, 677, 901, 935, 1082
HpaI GTTAAC 1 cut(s) 31
HpaII CCGG 3 cut(s) 310, 331, 394
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 8 cut(s) 204, 294, 456, 502, 556, 952, 984, 1081
Hpy188III TCNNGA 8 cut(s) 331, 674, 683, 722, 748, 801, 896, 1105
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 2 cut(s) 57, 634
HpyCH4III ACNGT 2 cut(s) 229, 496
HpyCH4V TGCA 6 cut(s) 132, 146, 842, 960, 1007, 1074
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 3 cut(s) 555, 777, 945
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 5 cut(s) 146, 512, 725, 932, 1155
HspAI GCGC 2 cut(s) 24, 161
KasI GGCGCC 1 cut(s) 23
Kpn2I TCCGGA 1 cut(s) 330
KspAI GTTAAC 1 cut(s) 31
Kzo9I GATC 6 cut(s) 335, 472, 709, 756, 1029, 1090
LmnI GCTCC 3 cut(s) 325, 333, 971
MaeI CTAG 2 cut(s) 674, 968
MaeIII GTNAC 6 cut(s) 476, 540, 809, 823, 1105, 1156
MalI GATC 6 cut(s) 337, 474, 711, 758, 1031, 1092
MboI GATC 6 cut(s) 335, 472, 709, 756, 1029, 1090
MboII GAAGA 3 cut(s) 77, 514, 704
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 2 cut(s) 335, 756
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 5 cut(s) 156, 220, 402, 858, 1142
Mly113I GGCGCC 1 cut(s) 24
MlyI GAGTC 2 cut(s) 370, 944
MnlI CCTC 4 cut(s) 264, 421, 776, 1112
MroI TCCGGA 1 cut(s) 330
MseI TTAA 4 cut(s) 30, 575, 732, 789
MslI CAYNNNNRTG 3 cut(s) 513, 1012, 1066
MspI CCGG 3 cut(s) 310, 331, 394
MspR9I CCNGG 6 cut(s) 311, 394, 395, 771, 885, 1111
MunI CAATTG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 844
MvaI CCWGG 3 cut(s) 771, 885, 1111
MvnI CGCG 1 cut(s) 482
MwoI GCNNNNNNNGC 1 cut(s) 158
NarI GGCGCC 1 cut(s) 24
NciI CCSGG 3 cut(s) 311, 394, 395
NdeII GATC 6 cut(s) 335, 472, 709, 756, 1029, 1090
NlaIII CATG 5 cut(s) 146, 512, 725, 932, 1155
NlaIV GGNNCC 3 cut(s) 25, 582, 758
NmeAIII GCCGAG 1 cut(s) 811
NmuCI GTSAC 2 cut(s) 823, 1105
NsbI TGCGCA 1 cut(s) 162
NspV TTCGAA 1 cut(s) 611
PagI TCATGA 1 cut(s) 721
PceI AGGCCT 1 cut(s) 943
PcsI WCGNNNNNNNCGW 1 cut(s) 88
PctI GAATGC 1 cut(s) 844
PfeI GAWTC 7 cut(s) 199, 205, 289, 668, 677, 901, 1082
PflFI GACNNNGTC 1 cut(s) 388
PfoI TCCNGGA 2 cut(s) 309, 883
PkrI GCNGC 2 cut(s) 109, 484
PleI GAGTC 2 cut(s) 369, 943
PluTI GGCGCC 1 cut(s) 27
PpsI GAGTC 2 cut(s) 369, 943
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 769, 883, 1109
PspGI CCWGG 3 cut(s) 769, 883, 1109
PspN4I GGNNCC 3 cut(s) 25, 582, 758
PspPI GGNCC 2 cut(s) 307, 850
PsuI RGATCY 2 cut(s) 335, 756
PsyI GACNNNGTC 1 cut(s) 388
RsaI GTAC 2 cut(s) 119, 506
RsaNI GTAC 2 cut(s) 118, 505
RseI CAYNNNNRTG 3 cut(s) 513, 1012, 1066
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 4 cut(s) 30, 575, 732, 789
SatI GCNGC 2 cut(s) 108, 483
Sau3AI GATC 6 cut(s) 335, 472, 709, 756, 1029, 1090
Sau96I GGNCC 2 cut(s) 307, 850
SchI GAGTC 2 cut(s) 370, 944
ScrFI CCNGG 6 cut(s) 311, 394, 395, 771, 885, 1111
SduI GDGCHC 1 cut(s) 330
SfoI GGCGCC 1 cut(s) 25
SfuI TTCGAA 1 cut(s) 611
SinI GGWCC 2 cut(s) 307, 850
SmaI CCCGGG 1 cut(s) 395
SmiMI CAYNNNNRTG 3 cut(s) 513, 1012, 1066
Sse9I AATT 5 cut(s) 156, 220, 402, 858, 1142
SseBI AGGCCT 1 cut(s) 943
SsiI CCGC 3 cut(s) 108, 182, 482
SspDI GGCGCC 1 cut(s) 23
SspMI CTAG 2 cut(s) 674, 968
SstI GAGCTC 1 cut(s) 330
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 6 cut(s) 309, 392, 393, 769, 883, 1109
StyI CCWWGG 1 cut(s) 998
TaaI ACNGT 2 cut(s) 229, 496
TaqI TCGA 4 cut(s) 91, 175, 208, 611
TasI AATT 5 cut(s) 156, 220, 402, 858, 1142
TatI WGTACW 1 cut(s) 117
TauI GCSGC 2 cut(s) 110, 485
TfiI GAWTC 7 cut(s) 199, 205, 289, 668, 677, 901, 1082
Tru1I TTAA 4 cut(s) 30, 575, 732, 789
Tru9I TTAA 4 cut(s) 30, 575, 732, 789
TscAI CASTG 1 cut(s) 1137
TseFI GTSAC 2 cut(s) 823, 1105
Tsp45I GTSAC 2 cut(s) 823, 1105
TspDTI ATGAA 8 cut(s) 77, 131, 582, 653, 672, 710, 1106, 1152
TspGWI ACGGA 1 cut(s) 1056
TspMI CCCGGG 1 cut(s) 393
TspRI CASTG 1 cut(s) 1137
Tth111I GACNNNGTC 1 cut(s) 388
VpaK11BI GGWCC 2 cut(s) 307, 850
XbaI TCTAGA 1 cut(s) 673
XmaI CCCGGG 1 cut(s) 393
XspI CTAG 2 cut(s) 674, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.