RLG00000016644

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012369
Physical Location & Seq
Reverse (-)
8532721 .. 8534936
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016644

Sequence Viewer

Length: 912 bp
ATGGCCGCCTATTTGTACAACCATAACTGTAAGGGATTTCATGCACAAGCCCCAATTCCGCACCCAAATCATCGATTCGCGGGTCTTGTTGGCCAGATTCGGAATCGAAAGACGGCCAATTTGACCGTGAAATGTTGCTTCAATAGTGGCAATCATAACAATAGAGTGAAGGGCAATGTGTTTGAGAATCCGAATGGGCAGTTGGTGCGAGATTTGGATTTGGAGCTCCGGAGATCCCAGAATGGTGGCTTTCAGGGGACTCATAATGGGTTTCACAGCCAGACCTTGTCCCGGGATAAAATTGTGGTTGCTGTTGATGTCGATGAGGTTTTGGGGAACTTTGTGTCGGCTCTGAACAGGTTTATTGCTGATCGTTACGCGGCTAACCATACGGTTTCGGAGTACCATGTGTATGAGTTCTTCAAGATATGGAAGTGTTCCCGTGATGAAGCCGATCTTCGTGTTCATGAGTTCTTTAAAACATCATATTTCAAGACTGGGATCCACCCCCTCCCAGGTGCTAAGGAGACGCTTAATAAGTTGTCAGGATTTTGTAACCTGTCAGTTGTGACTTCTCGGCAGAATGCAATCAAGGACCACACAATTCAATGGATTGAAAGTCATTTTCCAGGACTATTTCAGGAGATTCACTTCGGCAACCACTTTGCTCTACATGGAGAGTCAAGGCCTAAGTCAGAAATATGCAGGTCTCTAGGAGCAAAGGTTCTGATCGATGACAATCCAAGGTATGCACTTGAATGTGCCGAAGTTGGGATCAGGGTTCTACTTTTTGATTACGAGAACTCATATCCTTGGTGCAAGTCCGATTCTGTTGATCTACATCCCCTCGTGACCAGGGTTCATAACTGGGAAGCAGTGGAGAAGCAATTATTGGCATGGGTTACTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

304

Amino Acids

34.56

Weight (kDa)

8.37

Isoelectric Point (pI)

22.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT5C PF06941 101 - 295 1.3e-14 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33140
fragaria_vesca FvH4_1g09650 FvH4_1g09650
malus_domestica MD02G1105400.v1.1 MD15G1225300.v1.1
prunus_persica Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1 Prupe.7G189700_v2.0.a1
pyrus_communis pycom15g20010
rosa_chinensis RchiOBHm_Chr2g0096491
rosa_laevigata RLG00000016644
rosa_multiflora Rmu_sc0004096.1_g000012
rosa_roxburghii Rroxscaffold_2G00145600
rosa_rugosa Rorug02G0058100
rosa_samantha Rh2AG105100 Rh2BG107200 Rh2CG109000 Rh2DG108200
rosa_wichuraiana Rw2G008120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 696
AccII CGCG 2 cut(s) 80, 380
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 4 cut(s) 6, 59, 80, 380
AclWI GGATC 4 cut(s) 228, 496, 509, 782
AcoI YGGCCR 3 cut(s) 3, 91, 114
AfaI GTAC 2 cut(s) 17, 404
AfiI CCNNNNNNNGG 3 cut(s) 291, 515, 771
AgsI TTSAA 6 cut(s) 142, 424, 493, 608, 617, 758
AjnI CCWGG 3 cut(s) 514, 628, 854
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 875, 907
AloI GAACNNNNNNTCC 2 cut(s) 708, 740
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 521, 714
AlwI GGATC 4 cut(s) 228, 496, 509, 782
Ama87I CYCGRG 1 cut(s) 291
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 4 cut(s) 3, 91, 114, 686
ArsI GACNNNNNNTTYG 2 cut(s) 103, 135
AspS9I GGNCC 1 cut(s) 595
AsuC2I CCSGG 2 cut(s) 292, 293
AvaI CYCGRG 1 cut(s) 291
AvaII GGWCC 1 cut(s) 595
BalI TGGCCA 1 cut(s) 93
BamHI GGATCC 1 cut(s) 501
BanII GRGCYC 1 cut(s) 228
BarI GAAGNNNNNNTAC 2 cut(s) 404, 436
BauI CACGAG 1 cut(s) 848
Bbv12I GWGCWC 1 cut(s) 228
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 3 cut(s) 516, 630, 856
BcnI CCSGG 2 cut(s) 292, 293
BcoDI GTCTC 2 cut(s) 521, 714
BfaI CTAG 1 cut(s) 713
BfuAI ACCTGC 1 cut(s) 696
BisI GCNGC 2 cut(s) 6, 381
BlsI GCNGC 2 cut(s) 7, 382
Bme1390I CCNGG 5 cut(s) 292, 293, 516, 630, 856
Bme18I GGWCC 1 cut(s) 595
BmeT110I CYCGRG 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 595
BmiI GGNNCC 1 cut(s) 503
BmrFI CCNGG 5 cut(s) 292, 293, 516, 630, 856
BmrI ACTGGG 2 cut(s) 507, 877
BmuI ACTGGG 2 cut(s) 507, 877
Bpu10I CCTNAGC 1 cut(s) 522
BpuMI CCSGG 2 cut(s) 292, 293
Bsa29I ATCGAT 2 cut(s) 73, 732
BsaBI GATNNNNATC 2 cut(s) 738, 840
BsaI GGTCTC 1 cut(s) 714
BsaJI CCNNGG 5 cut(s) 291, 514, 743, 812, 855
BsaWI WCCGGW 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 708, 738
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 515, 771
Bse1I ACTGG 2 cut(s) 502, 872
Bse3DI GCAATG 1 cut(s) 181
Bse8I GATNNNNATC 2 cut(s) 738, 840
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 3 cut(s) 516, 630, 856
BseCI ATCGAT 2 cut(s) 73, 732
BseDI CCNNGG 5 cut(s) 291, 514, 743, 812, 855
BseGI GGATG 1 cut(s) 841
BseJI GATNNNNATC 2 cut(s) 738, 840
BseLI CCNNNNNNNGG 3 cut(s) 291, 515, 771
BseMI GCAATG 1 cut(s) 181
BseNI ACTGG 2 cut(s) 502, 872
Bsh1236I CGCG 2 cut(s) 80, 380
BshFI GGCC 4 cut(s) 5, 93, 116, 688
BshVI ATCGAT 2 cut(s) 73, 732
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 291
BsiSI CCGG 2 cut(s) 229, 292
BslFI GGGAC 2 cut(s) 271, 274
BslI CCNNNNNNNGG 3 cut(s) 291, 515, 771
BsmAI GTCTC 2 cut(s) 521, 714
BsmBI CGTCTC 1 cut(s) 521
BsmFI GGGAC 2 cut(s) 271, 274
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 4 cut(s) 5, 93, 116, 688
Bso31I GGTCTC 1 cut(s) 714
BsoBI CYCGRG 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 228
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 7 cut(s) 233, 370, 454, 501, 729, 774, 835
BspACI CCGC 4 cut(s) 6, 59, 80, 380
BspANI GGCC 4 cut(s) 5, 93, 116, 688
BspDI ATCGAT 2 cut(s) 73, 732
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 2 cut(s) 80, 380
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 503
BspMI ACCTGC 1 cut(s) 696
BspPI GGATC 4 cut(s) 228, 496, 509, 782
BspTNI GGTCTC 1 cut(s) 714
BsrDI GCAATG 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 2 cut(s) 502, 872
BssECI CCNNGG 5 cut(s) 291, 514, 743, 812, 855
BssMI GATC 7 cut(s) 233, 370, 454, 501, 729, 774, 835
BssSI CACGAG 1 cut(s) 848
BssT1I CCWWGG 2 cut(s) 743, 812
Bst2BI CACGAG 1 cut(s) 848
Bst2UI CCWGG 3 cut(s) 516, 630, 856
Bst4CI ACNGT 3 cut(s) 29, 127, 394
BstAPI GCANNNNNTGC 1 cut(s) 205
BstAUI TGTACA 1 cut(s) 15
BstDEI CTNAG 2 cut(s) 522, 690
BstF5I GGATG 1 cut(s) 841
BstFNI CGCG 2 cut(s) 80, 380
BstKTI GATC 7 cut(s) 236, 373, 457, 504, 732, 777, 838
BstMAI GTCTC 2 cut(s) 521, 714
BstMBI GATC 7 cut(s) 233, 370, 454, 501, 729, 774, 835
BstMWI GCNNNNNNNGC 1 cut(s) 205
BstNI CCWGG 3 cut(s) 516, 630, 856
BstSCI CCNGG 5 cut(s) 290, 291, 514, 628, 854
BstUI CGCG 2 cut(s) 80, 380
BstX2I RGATCY 2 cut(s) 233, 501
BstXI CCANNNNNNTGG 1 cut(s) 245
BstYI RGATCY 2 cut(s) 233, 501
Bsu15I ATCGAT 2 cut(s) 73, 732
BsuRI GGCC 4 cut(s) 5, 93, 116, 688
BsuTUI ATCGAT 2 cut(s) 73, 732
BtsCI GGATG 1 cut(s) 841
BtsI GCAGTG 1 cut(s) 882
BtsIMutI CAGTG 1 cut(s) 882
BveI ACCTGC 1 cut(s) 696
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 595
Cfr9I CCCGGG 1 cut(s) 291
ClaI ATCGAT 2 cut(s) 73, 732
CseI GACGC 1 cut(s) 538
Csp6I GTAC 2 cut(s) 16, 403
CviAII CATG 5 cut(s) 41, 407, 467, 674, 897
CviQI GTAC 2 cut(s) 16, 403
DdeI CTNAG 2 cut(s) 522, 690
DpnI GATC 7 cut(s) 235, 372, 456, 503, 731, 776, 837
DpnII GATC 7 cut(s) 233, 370, 454, 501, 729, 774, 835
DraI TTTAAA 1 cut(s) 478
EaeI YGGCCR 3 cut(s) 3, 91, 114
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 2 cut(s) 743, 812
Eco147I AGGCCT 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 714
Eco47I GGWCC 1 cut(s) 595
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 226
EcoRII CCWGG 3 cut(s) 514, 628, 854
EcoT14I CCWWGG 2 cut(s) 743, 812
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 2 cut(s) 743, 812
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 5 cut(s) 44, 410, 470, 677, 900
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 2 cut(s) 271, 274
FatI CATG 5 cut(s) 40, 406, 466, 673, 896
FauI CCCGC 1 cut(s) 73
Fnu4HI GCNGC 2 cut(s) 6, 381
FokI GGATG 1 cut(s) 828
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 2 cut(s) 6, 381
FspBI CTAG 1 cut(s) 713
GluI GCNGC 2 cut(s) 6, 381
HaeIII GGCC 4 cut(s) 5, 93, 116, 688
HapII CCGG 2 cut(s) 229, 292
HgaI GACGC 1 cut(s) 538
Hin1II CATG 5 cut(s) 44, 410, 470, 677, 900
HinfI GANTC 8 cut(s) 75, 97, 103, 187, 259, 646, 680, 827
HpaII CCGG 2 cut(s) 229, 292
Hpy188I TCNGA 7 cut(s) 102, 192, 354, 400, 697, 729, 826
Hpy188III TCNNGA 7 cut(s) 229, 424, 467, 493, 546, 641, 850
HpyAV CCTTC 1 cut(s) 163
HpyCH4III ACNGT 3 cut(s) 29, 127, 394
HpyCH4V TGCA 5 cut(s) 44, 587, 705, 752, 819
HpyF10VI GCNNNNNNNGC 1 cut(s) 205
HpyF3I CTNAG 2 cut(s) 522, 690
Hsp92II CATG 5 cut(s) 44, 410, 470, 677, 900
Kpn2I TCCGGA 1 cut(s) 228
Kzo9I GATC 7 cut(s) 233, 370, 454, 501, 729, 774, 835
LmnI GCTCC 3 cut(s) 223, 231, 716
MaeI CTAG 1 cut(s) 713
MaeIII GTNAC 5 cut(s) 374, 554, 568, 850, 901
MalI GATC 7 cut(s) 235, 372, 456, 503, 731, 776, 837
MboI GATC 7 cut(s) 233, 370, 454, 501, 729, 774, 835
MboII GAAGA 2 cut(s) 412, 449
MflI RGATCY 2 cut(s) 233, 501
MhlI GDGCHC 1 cut(s) 228
MlsI TGGCCA 1 cut(s) 93
MluCI AATT 5 cut(s) 54, 118, 300, 603, 887
MluNI TGGCCA 1 cut(s) 93
MlyI GAGTC 2 cut(s) 253, 689
MnlI CCTC 3 cut(s) 319, 521, 857
Mox20I TGGCCA 1 cut(s) 93
MroI TCCGGA 1 cut(s) 228
MscI TGGCCA 1 cut(s) 93
MseI TTAA 2 cut(s) 477, 534
MslI CAYNNNNRTG 2 cut(s) 411, 757
Msp20I TGGCCA 1 cut(s) 93
MspI CCGG 2 cut(s) 229, 292
MspR9I CCNGG 5 cut(s) 292, 293, 516, 630, 856
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 3 cut(s) 516, 630, 856
MvnI CGCG 2 cut(s) 80, 380
MwoI GCNNNNNNNGC 1 cut(s) 205
NciI CCSGG 2 cut(s) 292, 293
NdeII GATC 7 cut(s) 233, 370, 454, 501, 729, 774, 835
NlaIII CATG 5 cut(s) 44, 410, 470, 677, 900
NlaIV GGNNCC 1 cut(s) 503
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 2 cut(s) 568, 850
PagI TCATGA 1 cut(s) 466
PceI AGGCCT 1 cut(s) 688
PctI GAATGC 1 cut(s) 589
PfeI GAWTC 6 cut(s) 75, 97, 103, 187, 646, 827
PflFI GACNNNGTC 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 628
PkrI GCNGC 2 cut(s) 7, 382
PleI GAGTC 2 cut(s) 253, 688
PpsI GAGTC 2 cut(s) 253, 688
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 514, 628, 854
PspGI CCWGG 3 cut(s) 514, 628, 854
PspN4I GGNNCC 1 cut(s) 503
PspPI GGNCC 1 cut(s) 595
PsuI RGATCY 2 cut(s) 233, 501
PsyI GACNNNGTC 1 cut(s) 286
RsaI GTAC 2 cut(s) 17, 404
RsaNI GTAC 2 cut(s) 16, 403
RseI CAYNNNNRTG 2 cut(s) 411, 757
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 2 cut(s) 477, 534
SatI GCNGC 2 cut(s) 6, 381
Sau3AI GATC 7 cut(s) 233, 370, 454, 501, 729, 774, 835
Sau96I GGNCC 1 cut(s) 595
SchI GAGTC 2 cut(s) 253, 689
ScrFI CCNGG 5 cut(s) 292, 293, 516, 630, 856
SduI GDGCHC 1 cut(s) 228
SetI ASST 9 cut(s) 228, 287, 330, 362, 520, 561, 710, 726, 749
SinI GGWCC 1 cut(s) 595
SmaI CCCGGG 1 cut(s) 293
SmiMI CAYNNNNRTG 2 cut(s) 411, 757
Sse9I AATT 5 cut(s) 54, 118, 300, 603, 887
SseBI AGGCCT 1 cut(s) 688
SsiI CCGC 4 cut(s) 6, 59, 80, 380
SspMI CTAG 1 cut(s) 713
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 688
StyD4I CCNGG 5 cut(s) 290, 291, 514, 628, 854
StyI CCWWGG 2 cut(s) 743, 812
TaaI ACNGT 3 cut(s) 29, 127, 394
TaqI TCGA 4 cut(s) 73, 106, 321, 732
TasI AATT 5 cut(s) 54, 118, 300, 603, 887
TatI WGTACW 1 cut(s) 15
TauI GCSGC 2 cut(s) 8, 383
TfiI GAWTC 6 cut(s) 75, 97, 103, 187, 646, 827
Tru1I TTAA 2 cut(s) 477, 534
Tru9I TTAA 2 cut(s) 477, 534
TscAI CASTG 1 cut(s) 882
TseFI GTSAC 2 cut(s) 568, 850
Tsp45I GTSAC 2 cut(s) 568, 850
TspDTI ATGAA 5 cut(s) 29, 455, 462, 851, 897
TspMI CCCGGG 1 cut(s) 291
TspRI CASTG 1 cut(s) 882
Tth111I GACNNNGTC 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 595
XmaI CCCGGG 1 cut(s) 291
XspI CTAG 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.