Rorug02G0058100

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001454
Physical Location & Seq
Forward (+)
4480540 .. 4483055
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0058100.1

Sequence Viewer

Length: 729 bp
ATGAAAGAAAAGGAGCAGTTCGAACTAAACCTTGGCAGACTCTTGACTCTGGGTGTTTCGTCAGGGACTCTCAAGATGAAGCTCAAGCAGCTCGAAAGCCTCCTTGGTGGTCTCCAACAGTTCTCCAACCCAAAGGTGGAGCTGGAACAATACCCAACTGGACCCCACATTGCATCTCGCATGCTCTACACTGCAGAGAATTCATTTGGGGATGTGTGTGACAAGGTAGTGGCTGATTTTGGCTGTGGTTGTGGTACATTGGGGGTTGCAGCTGGACTCTTGGGCGCAGAACATGTGATTGGCATTGACGTCGATCCAGAATCTCTTGAATTAGCTTCACTAAATGCTGAGGAACTTGAGTTGGACATAGATTATATTCAATGCAACATCAAGAACTTAGGGTGGAGAGCTGAAGTTGTTGATACTATTGTAATGAATCCTCCATTTGGAACTCGGAAAAAGGGTGCTGACATGGATTTTCTCTCTGTGGCTTTGAAGGTTGCTTCTCAAGCTGTTTATTCCTTACATAAAACCTCCACGAGAGATCATGTGAAAAGGGCAGCCTTGCAGCAGTTCAATGCTAGCAGTGCTGAGGTTATATGTGAGCTTCGATATGATTTACCACAAACGTACAAGTTTCATAAGAAACGAGAGGTGGATATCGCTGTGGACTTGTGGCGATTTGTCCCCAAGAGTAAGCAGGGGTCCAGAGATCTAGCACATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

242

Amino Acids

26.76

Weight (kDa)

5.9

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 53 - 146 7e-11 Ribosomal protein L11 methyltransferase (PrmA)
MTS PF05175 73 - 154 5e-12 Methyltransferase small domain
Methyltransf_31 PF13847 75 - 149 6.3e-09 Methyltransferase domain
UPF0020 PF01170 75 - 154 3.3e-07 RMKL-like, methyltransferase domain
Methyltransf_25 PF13649 77 - 146 8.3e-10 Methyltransferase domain
Methyltransf_11 PF08241 79 - 146 3.3e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
AclWI GGATC 1 cut(s) 308
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 432
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 2 cut(s) 256, 632
AfiI CCNNNNNNNGG 2 cut(s) 136, 446
AflIII ACRYGT 2 cut(s) 292, 721
AgsI TTSAA 4 cut(s) 329, 380, 496, 577
AjuI GAANNNNNNNTTGG 6 cut(s) 15, 47, 87, 119, 282, 314
AluBI AGCT 8 cut(s) 82, 91, 142, 272, 335, 410, 512, 607
AluI AGCT 8 cut(s) 82, 91, 142, 272, 335, 410, 512, 607
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 1 cut(s) 308
ApeKI GCWGC 4 cut(s) 88, 269, 560, 568
ApoI RAATTY 1 cut(s) 199
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 161, 705
AsuII TTCGAA 1 cut(s) 21
AsuNHI GCTAGC 1 cut(s) 581
AvaII GGWCC 2 cut(s) 161, 705
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BauI CACGAG 1 cut(s) 538
BbvCI CCTCAGC 2 cut(s) 348, 591
BbvI GCAGC 4 cut(s) 100, 281, 572, 580
BcgI CGANNNNNNTGC 2 cut(s) 292, 326
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 2 cut(s) 582, 716
BfmI CTRYAG 1 cut(s) 192
BglII AGATCT 1 cut(s) 712
BisI GCNGC 4 cut(s) 89, 270, 561, 569
BlsI GCNGC 4 cut(s) 90, 271, 562, 570
Bme18I GGWCC 2 cut(s) 161, 705
BmgT120I GGNCC 2 cut(s) 161, 705
BmiI GGNNCC 2 cut(s) 163, 706
BmsI GCATC 1 cut(s) 182
BmtI GCTAGC 1 cut(s) 585
Bpu10I CCTNAGC 2 cut(s) 348, 591
Bpu14I TTCGAA 1 cut(s) 21
BpuEI CTTGAG 4 cut(s) 56, 68, 377, 492
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 31, 103
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 446
Bse1I ACTGG 1 cut(s) 163
Bse3DI GCAATG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 31, 103
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 136, 446
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 339, 582
BseNI ACTGG 1 cut(s) 163
BseXI GCAGC 4 cut(s) 100, 281, 572, 580
BslFI GGGAC 2 cut(s) 79, 671
BslI CCNNNNNNNGG 2 cut(s) 136, 446
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 2 cut(s) 79, 671
Bso31I GGTCTC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 21
Bsp143I GATC 3 cut(s) 313, 544, 712
BspCNI CTCAG 2 cut(s) 340, 583
BspLI GGNNCC 2 cut(s) 163, 706
BspMAI CTGCAG 1 cut(s) 196
BspOI GCTAGC 1 cut(s) 585
BspPI GGATC 1 cut(s) 308
BspT104I TTCGAA 1 cut(s) 21
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 2 cut(s) 31, 103
BssMI GATC 3 cut(s) 313, 544, 712
BssNI GRCGYC 1 cut(s) 309
BssSI CACGAG 1 cut(s) 538
BssT1I CCWWGG 2 cut(s) 31, 103
Bst2BI CACGAG 1 cut(s) 538
Bst4CI ACNGT 1 cut(s) 120
BstACI GRCGYC 1 cut(s) 309
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 182, 583
BstDEI CTNAG 3 cut(s) 348, 397, 591
BstF5I GGATG 1 cut(s) 217
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 3 cut(s) 316, 547, 715
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 3 cut(s) 313, 544, 712
BstMWI GCNNNNNNNGC 3 cut(s) 88, 509, 587
BstNSI RCATGY 3 cut(s) 184, 296, 725
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 4 cut(s) 100, 281, 572, 580
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BtsCI GGATG 1 cut(s) 217
BtsI GCAGTG 2 cut(s) 189, 592
BtsIMutI CAGTG 2 cut(s) 189, 592
Cac8I GCNNGC 2 cut(s) 182, 583
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 161, 705
Csp6I GTAC 2 cut(s) 255, 631
CviAII CATG 5 cut(s) 181, 293, 472, 548, 722
CviQI GTAC 2 cut(s) 255, 631
DdeI CTNAG 3 cut(s) 348, 397, 591
DpnI GATC 3 cut(s) 315, 546, 714
DpnII GATC 3 cut(s) 313, 544, 712
Eco130I CCWWGG 2 cut(s) 31, 103
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 161, 705
Eco57I CTGAAG 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 199
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 2 cut(s) 31, 103
ErhI CCWWGG 2 cut(s) 31, 103
FaeI CATG 5 cut(s) 184, 296, 475, 551, 725
FaqI GGGAC 2 cut(s) 79, 671
FatI CATG 5 cut(s) 180, 292, 471, 547, 721
Fnu4HI GCNGC 4 cut(s) 89, 270, 561, 569
FokI GGATG 1 cut(s) 224
Fsp4HI GCNGC 4 cut(s) 89, 270, 561, 569
FspBI CTAG 2 cut(s) 582, 716
GlaI GCGC 1 cut(s) 286
GluI GCNGC 4 cut(s) 89, 270, 561, 569
HhaI GCGC 1 cut(s) 287
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 5 cut(s) 184, 296, 475, 551, 725
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 6 cut(s) 39, 46, 67, 276, 320, 436
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 1 cut(s) 456
Hpy188III TCNNGA 6 cut(s) 43, 73, 317, 326, 391, 708
Hpy8I GTNNAC 1 cut(s) 670
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 1 cut(s) 120
HpyCH4IV ACGT 2 cut(s) 309, 629
HpyCH4V TGCA 5 cut(s) 173, 194, 269, 384, 568
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 509, 587
HpyF3I CTNAG 3 cut(s) 348, 397, 591
HpySE526I ACGT 2 cut(s) 309, 629
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 5 cut(s) 184, 296, 475, 551, 725
HspAI GCGC 1 cut(s) 285
Kzo9I GATC 3 cut(s) 313, 544, 712
LmnI GCTCC 2 cut(s) 13, 139
LpnPI CCDG 8 cut(s) 35, 48, 128, 144, 258, 330, 686, 721
Lsp1109I GCAGC 4 cut(s) 100, 281, 572, 580
LweI GCATC 1 cut(s) 182
MaeI CTAG 2 cut(s) 582, 716
MaeII ACGT 2 cut(s) 309, 629
MaeIII GTNAC 1 cut(s) 218
MalI GATC 3 cut(s) 315, 546, 714
MboI GATC 3 cut(s) 313, 544, 712
MflI RGATCY 1 cut(s) 712
MluCI AATT 2 cut(s) 199, 329
MlyI GAGTC 4 cut(s) 33, 40, 61, 270
MmeI TCCRAC 3 cut(s) 139, 150, 342
MnlI CCTC 6 cut(s) 110, 343, 450, 544, 586, 646
MspA1I CMGCKG 1 cut(s) 272
MwoI GCNNNNNNNGC 3 cut(s) 88, 509, 587
NdeII GATC 3 cut(s) 313, 544, 712
NheI GCTAGC 1 cut(s) 581
NlaIII CATG 5 cut(s) 184, 296, 475, 551, 725
NlaIV GGNNCC 2 cut(s) 163, 706
NmuCI GTSAC 1 cut(s) 218
NspI RCATGY 3 cut(s) 184, 296, 725
NspV TTCGAA 1 cut(s) 21
PaeI GCATGC 1 cut(s) 184
PciI ACATGT 2 cut(s) 292, 721
PfeI GAWTC 2 cut(s) 320, 436
PkrI GCNGC 4 cut(s) 90, 271, 562, 570
PleI GAGTC 4 cut(s) 33, 40, 61, 270
PpsI GAGTC 4 cut(s) 33, 40, 61, 270
PscI ACATGT 2 cut(s) 292, 721
PspN4I GGNNCC 2 cut(s) 163, 706
PspPI GGNCC 2 cut(s) 161, 705
PstI CTGCAG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 712
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 2 cut(s) 256, 632
RsaNI GTAC 2 cut(s) 255, 631
SatI GCNGC 4 cut(s) 89, 270, 561, 569
Sau3AI GATC 3 cut(s) 313, 544, 712
Sau96I GGNCC 2 cut(s) 161, 705
SchI GAGTC 4 cut(s) 33, 40, 61, 270
SfaNI GCATC 1 cut(s) 182
SfcI CTRYAG 1 cut(s) 192
SfuI TTCGAA 1 cut(s) 21
SinI GGWCC 2 cut(s) 161, 705
SmlI CTYRAG 4 cut(s) 71, 83, 356, 507
SmoI CTYRAG 4 cut(s) 71, 83, 356, 507
SphI GCATGC 1 cut(s) 184
Sse9I AATT 2 cut(s) 199, 329
SspMI CTAG 2 cut(s) 582, 716
StyI CCWWGG 2 cut(s) 31, 103
TaaI ACNGT 1 cut(s) 120
TaiI ACGT 2 cut(s) 312, 632
TaqI TCGA 4 cut(s) 21, 93, 312, 610
TasI AATT 2 cut(s) 199, 329
TfiI GAWTC 2 cut(s) 320, 436
TscAI CASTG 2 cut(s) 196, 592
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 4 cut(s) 88, 269, 560, 568
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 5 cut(s) 17, 92, 192, 449, 629
TspRI CASTG 2 cut(s) 196, 592
VpaK11BI GGWCC 2 cut(s) 161, 705
XapI RAATTY 1 cut(s) 199
XceI RCATGY 3 cut(s) 184, 296, 725
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XspI CTAG 2 cut(s) 582, 716
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.