Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0001454

Physical Location & Seq

Genomic Coordinates Forward (+)

8905507 .. 8912473

Gene Structure 6967 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG108100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3159 bp

ATGCTTGGAAATGGGGTCGTTGGGATCGTCTCGGAGACTGTGAACAAGTGGGAAAGAAGAGCGCCATTGACCCCCTCTCACTGTGCACGGCTGCTTCACAGTGGCAGTGACAAAACCGGCGTGTCTCGGATCATCGTGCAGCCGTCGACGAAGCGCATCCATCATGATGCATTGTATGAAGAAGTTGGATGTGAGATTTCAGAGGACCTGTCAGAATGTGGCCTCATTTTGGGGGTCAAACAACCCAAGCCGGAGATGATTCTTCCGGATAGAGCTTATGCTTTTTTCTCACATACCCACAAGGCGCCGAAAGAAAATATGCCATTGTTGGATAAGATTCTAGCAGAAAGGGTGTCTTTGTATGATTATGAGCTAATTGTTGGGGATCATGGGAGGAGACTGCTTGCATTCGGCAAGTTTGCTGGTAGAGCTGGATTTATCGACTTTTTGCGCGGCTTAGGACAAAGGTACCTAAGTCTTGGATATTCGACACCATTTCTTTCGTTGGGTGCTTCGTACATGTATTCATCTTTGGCTGCTGCCAAGGCTGCAGTAATTTCTGTTGGAGAAGAGATAGCAACTCAGGGATTGCCGGCAGGAATCTGCCCTCTCGTCTTTGTCTTCACCGGTTCAGGAAATGTTTCTTCTGGTGCACAAGAGATATTCAAGCTTCTCCCTCATACTTTTGTGGAGCCAAGCAGACTCCCAGGGATATTTGGGACGGCCAAGGATGCTGCTCAACCCACCAGGACGTCAAAGAGGGTCTTCCAAGTATGTGGTTGTGTTGTGACTTGTAAAGACATGGTTGAACACAAAGATCCCAAAAAATCATTTGACAAAGCTGATTATTATGTACATCCAGAACACTACAACCCGGTTTTCCATGAAAATATAGCTCCTTATGCATCTGTAATCGTGAATTGTATGTATTGGGAGAAAAGATATCCTCGATTATTGAGTACAAAGCAGTTTCAAGATTTAACAAGGAAAGGATGTAAGCTTGTTGGTATCTCTGATATAACTTGTGATATAGGGGGATCAATAGAGTTTGTTAATCAAACCACACAAATTGACTCACCTTTCTTCAGATATGATCCAATAAAAGATTCTTACCATCAGGACATGGAAGGTGATGGTGTGGTATGTTCAGCTGTGGACATTCTTCCAACAGAATTTGCAAAAGAGGCTTCCAAACACTTTGGAGACATATTATCCGAATTTGTTGGCTATTTGGCTTCTACAAAAGACATTAAAAAGTTACCTGCACACTTGATGAAAGCTTGCATAGCCCATGGAGGAACACTTACCCCATTGTACGAATACATATCTCGCATGCGAAAATCTGATGACACAGAAGAAACATCAAAAGATCATGCTAGTCACCACTTTAACAAGAAGTACACCACATTAGTATCTCTCAGTGGTCACTTGTTTGATCAATTTCTGATAAATGAGGCCTTAGATATTATTGAAGCTGCAAGTGGATCCTTCCACTTGGTGAAGTGTCAAGTTGGTCCAAGTTCTCATGCTATGTCATACTCAGAACTCGAAGTTGGTGCAGATGATGAGGAAGTTCTGAATAAAATTATCGATTCTTTGACTTCTCTTGCTAATCCAAATGAAAATCAACCGTTAAAGCAAGAAGCAAATAAGATTTCACTGAGGGTTGGTAAAGTCCAGGATAGTGGAGCTAAGAAGGAAAATGACACGAAAAAGGTCGGGATTCTAATTATTGGAGCAGGCCGGGTCTGTCAACCAGCTGCTGAAATGCTAGCATCAATTGGTGGAATGTCTTCCCAACAATGGTATAAAACATGCATGGAAGGTGACTTTGAGGAAGACATTGATGTGCAAGTTACTGTTGCATCTCTCTATCTAAAGGATGCTGAAGAGATCACTGAAGGTATTCCAAATGCAATTCCAGTTCAACTTGATGTGTCCGACACTAGTACTCTTCATAAGTATATATCTGAGGCTGAAGTTGTTATAAGCTTACTGCCTGCTTTTTGTCACATTACTGTAGCAACTGCATGTATTGAGCTCAAAAAGCACCTTGTCACTGCTAGCTATGTTGATGATGCCATGTCAGAGCTAAATGAAAAGGCAAAGACTGCCGGTATTACAATTCTTGGTGAGCTGGGCTTGGACCCTGGGATAGATCACATGATGGCGATGAAGATGATCAACCAAGCACATGTTCGTAAGGGGAAAATAAAGTCTTTTACTTCTTACTGCGGTGGACTCCCATCCCCAGCTGCAGCAAACAATCCTCTAGCATATAAGTTCAGCTGGAGTCCTGCAGGAGCTATCCGAGCTGGATGCAATCCTGCCACTTATAAATCTAATGGCGAAATCTTAAATGTTGATGGGAAGAATCTATATGATTCAGCTGTGAAACATCGGATACCTGACCTTCCAGCTTTTGCCTTGGAATGCCTTCCAAATCGTAATTCACTAGTTTATGGGGACTTGTATGGCATTGGAAAGGAAGCATCAACCGTCTTTCGTGGAACCCTTCGCTATGAAGGGTTTGGCCAAATTATGGGAACACTGTCAAGAATTGGTTTATTTGAATCCGAACCTCATCCACTTTTTAAGGATGGAAAGAAACCAACATTCCGGATGTTCTTGTCCGAACTTCTAAAAATCAAAAGCGAAGAAGTGGATGGATCTCTGAGAGGAGAGAAAGCCATCAGCGAAAGGATTATCAGTCTTGGATACAGCAAACAGCAAGAAACTGCAGTACGGGTAGCTAAAACTATCATATTTTTGGGACTGCATGAGCAGAAAGAAATTCCTGCCTCCTGCAAAAGTGCTTTTGATGTCAGTTGTCTCCTCATGGAAGATAGGTTAGCATACACGAGCACAGAACAGGATATGGTACTTCTGCACCATGAAGTGGAGGTTGAGTTCCCAGATTCTAAACTCATAGAGAAACAGAGTGCCACTTTACTGGAATTTGGGACGATGAGGAATGGTAAAATGGTCACCGCCATGGCTTTCACTGTTGGTATCCCGGCAGCCATTGGAGCTCTGCTCCTACTTGGAAACAAGATAAAAACAAGAGGTGTCTTAAGGCCTCTTGAACCTGAAGTATATGTCCCAGCCATGGATATCATACAGGCATATGGTATCAAGGTAATGGAGAAGGTCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004753 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006091 GO:0006520 GO:0006553 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009085 GO:0009987 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0019752 GO:0019878 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00300 ko00310 ko01100 ko01110 ko01130 ko01230 map00300 map00310 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

AlaDh_PNT_N Saccharop_dh_N Sacchrp_dh_C Sacchrp_dh_NADP

Protein Analysis

1052

Amino Acids

115.57

Weight (kDa)

6.09

Isoelectric Point (pI)

38.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AlaDh_PNT_N	PF05222	8 - 143	9.8e-20	Alanine dehydrogenase/PNT, N-terminal domain
Saccharop_dh_N	PF04455	470 - 540	7.2e-19	LOR/SDH bifunctional enzyme conserved region
Sacchrp_dh_NADP	PF03435	575 - 710	2.2e-18	Saccharopine dehydrogenase NADP binding domain
Sacchrp_dh_C	PF16653	714 - 1045	1e-79	Saccharopine dehydrogenase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001454)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33150 AT4G33150 AT4G33150 AT4G33150
fragaria_vesca	FvH4_1g09640 FvH4_1g09640 FvH4_1g09640 FvH4_1g09640 FvH4_1g09640 FvH4_1g09640 FvH4_1g09640 FvH4_1g09640
malus_domestica	MD02G1105000.v1.1 MD02G1105300.v1.1
prunus_persica	Prupe.7G189800_v2.0.a1 Prupe.7G189800_v2.0.a1 Prupe.7G189800_v2.0.a1
pyrus_communis	pycom02g08340
rosa_chinensis	RchiOBHm_Chr2g0096481 RchiOBHm_Chr2g0121231 RchiOBHm_Chr2g0121241 RchiOBHm_Chr2g0169881 RchiOBHm_Chr2g0169891
rosa_laevigata	RLG00000016643
rosa_multiflora	Rmu_sc0004096.1_g000015 Rmu_sc0018708.1_g000006
rosa_roxburghii	Rroxscaffold_176G00431240 Rroxscaffold_2G00145610 Rroxscaffold_7G00171930
rosa_rugosa	Rorug02G0058100
rosa_samantha	Rh1AG160500 Rh1AG160600 Rh1CG150200 Rh2AG105000 Rh2AG293500 Rh2AG293600 Rh2BG107100 Rh2CG108900 Rh2CG232200 Rh2DG108100 Rh2DG317400 Rh2DG317500 Rh3AG001100 Rh3BG000800 Rh3CG000800 Rh3DG001000 Rh4AG204900 Rh7AG127200
rosa_wichuraiana	Rw2G008110 Rw2G016820 Rw6G021060 Rw6G033110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1988, 2337
AatII	GACGTC	1 cut(s)	755
Acc36I	ACCTGC	1 cut(s)	1270
Acc65I	GGTACC	1 cut(s)	468
AccB1I	GGYRCC	2 cut(s)	304, 468
AccB7I	CCANNNNNTGG	2 cut(s)	2542, 2899
AccI	GTMKAC	1 cut(s)	146
AccII	CGCG	1 cut(s)	453
AccIII	TCCGGA	2 cut(s)	265, 2619
AciI	CCGC	3 cut(s)	453, 2235, 2991
AclWI	GGATC	9 cut(s)	32, 137, 393, 812, 1045, 1088, 1479, 1492, 2677
AcoI	YGGCCR	2 cut(s)	723, 2533
AcsI	RAATTY	4 cut(s)	1172, 1217, 2793, 2957
AcuI	CTGAAG	5 cut(s)	1069, 1908, 1920, 1998, 3111
AcyI	GRCGYC	2 cut(s)	305, 752
AdeI	CACNNNGTG	1 cut(s)	1498
AfaI	GTAC	9 cut(s)	470, 518, 855, 961, 1316, 1400, 1951, 2745, 2883
AfiI	CCNNNNNNNGG	5 cut(s)	229, 1803, 2542, 2899, 3109
AflII	CTTAAG	1 cut(s)	3073
AflIII	ACRYGT	2 cut(s)	519, 2194
AgeI	ACCGGT	1 cut(s)	626
AgsI	TTSAA	7 cut(s)	667, 809, 974, 1472, 1928, 2573, 3086
AhlI	ACTAGT	2 cut(s)	1946, 2455
AjnI	CCWGG	4 cut(s)	706, 746, 1677, 2149
AjuI	GAANNNNNNNTTGG	8 cut(s)	1090, 1122, 1536, 1568, 2181, 2213, 2434, 2466
AloI	GAACNNNNNNTCC	2 cut(s)	2894, 2926
Alw21I	GWGCWC	5 cut(s)	88, 655, 2043, 2867, 3034
Alw26I	GTCTC	6 cut(s)	29, 34, 129, 391, 1197, 2837
Alw44I	GTGCAC	2 cut(s)	84, 651
AlwI	GGATC	9 cut(s)	32, 137, 393, 812, 1045, 1088, 1479, 1492, 2677
AlwNI	CAGNNNCTG	1 cut(s)	1763
Aor13HI	TCCGGA	2 cut(s)	265, 2619
AoxI	GGCC	6 cut(s)	220, 723, 1455, 1741, 2533, 3077
ApaLI	GTGCAC	2 cut(s)	84, 651
ApoI	RAATTY	4 cut(s)	1172, 1217, 2793, 2957
AsiGI	ACCGGT	1 cut(s)	626
Asp700I	GAANNNNTTC	4 cut(s)	640, 1792, 1905, 2436
Asp718I	GGTACC	1 cut(s)	468
AspLEI	GCGC	4 cut(s)	64, 156, 307, 453
AspS9I	GGNCC	3 cut(s)	205, 1514, 2146
AsuC2I	CCSGG	3 cut(s)	875, 1745, 3017
AsuHPI	GGTGA	8 cut(s)	616, 1068, 1142, 1373, 1510, 1838, 2144, 2980
AsuNHI	GCTAGC	2 cut(s)	1771, 2063
AvaII	GGWCC	3 cut(s)	205, 1514, 2146
BaeGI	GKGCMC	2 cut(s)	88, 655
BaeI	ACNNNNGTAYC	6 cut(s)	452, 460, 485, 493, 1395, 1428
BalI	TGGCCA	1 cut(s)	2535
BamHI	GGATCC	1 cut(s)	1484
BanI	GGYRCC	2 cut(s)	304, 468
BanII	GRGCYC	2 cut(s)	2043, 3034
BauI	CACGAG	1 cut(s)	2860
BbsI	GAAGAC	4 cut(s)	613, 757, 1785, 1845
Bbv12I	GWGCWC	5 cut(s)	88, 655, 2043, 2867, 3034
BccI	CCATC	9 cut(s)	168, 1122, 1127, 2161, 2254, 2360, 2594, 2660, 2699
BceAI	ACGGC	3 cut(s)	104, 127, 738
BcgI	CGANNNNNNTGC	4 cut(s)	1538, 1572, 2301, 2335
BciT130I	CCWGG	4 cut(s)	708, 748, 1679, 2151
BciVI	GTATCC	3 cut(s)	2397, 2711, 3023
BclI	TGATCA	2 cut(s)	1435, 2181
BcnI	CCSGG	3 cut(s)	875, 1745, 3017
BcoDI	GTCTC	6 cut(s)	29, 34, 129, 391, 1197, 2837
BcuI	ACTAGT	2 cut(s)	1946, 2455
BfaI	CTAG	7 cut(s)	341, 1377, 1772, 1947, 2064, 2273, 2456
BfmI	CTRYAG	5 cut(s)	549, 2019, 2256, 2298, 2739
BfoI	RGCGCY	2 cut(s)	65, 308
BfrI	CTTAAG	1 cut(s)	3073
BfuAI	ACCTGC	1 cut(s)	1270
BfuI	GTATCC	3 cut(s)	2397, 2711, 3023
BmcAI	AGTACT	1 cut(s)	1951
Bme1390I	CCNGG	7 cut(s)	708, 748, 875, 1679, 1745, 2151, 3017
Bme18I	GGWCC	3 cut(s)	205, 1514, 2146
BmgT120I	GGNCC	3 cut(s)	205, 1514, 2146
BmiI	GGNNCC	6 cut(s)	306, 470, 693, 1486, 2148, 2512
BmrFI	CCNGG	7 cut(s)	708, 748, 875, 1679, 1745, 2151, 3017
BmtI	GCTAGC	2 cut(s)	1775, 2067
BpiI	GAAGAC	4 cut(s)	613, 757, 1785, 1845
BplI	GAGNNNNNCTC	2 cut(s)	3021, 3053
BpmI	CTGGAG	1 cut(s)	2311
Bpu10I	CCTNAGC	1 cut(s)	457
BpuMI	CCSGG	3 cut(s)	875, 1745, 3017
Bsa29I	ATCGAT	1 cut(s)	1590
BsaHI	GRCGYC	2 cut(s)	305, 752
BsaWI	WCCGGW	3 cut(s)	265, 626, 2619
BsaXI	ACNNNNNCTCC	2 cut(s)	2894, 2924
Bsc4I	CCNNNNNNNGG	5 cut(s)	229, 1803, 2542, 2899, 3109
Bse118I	RCCGGY	4 cut(s)	116, 592, 626, 2114
Bse1I	ACTGG	2 cut(s)	1922, 2958
BseAI	TCCGGA	2 cut(s)	265, 2619
BseBI	CCWGG	4 cut(s)	708, 748, 1679, 2151
BseCI	ATCGAT	1 cut(s)	1590
BseLI	CCNNNNNNNGG	5 cut(s)	229, 1803, 2542, 2899, 3109
BseMII	CTCAG	6 cut(s)	596, 1432, 1554, 1652, 1962, 2666
BseNI	ACTGG	2 cut(s)	1922, 2958
BseRI	GAGGAG	3 cut(s)	409, 2694, 2825
BseSI	GKGCMC	2 cut(s)	88, 655
BseYI	CCCAGC	3 cut(s)	2137, 2251, 3103
BsgI	GTGCAG	4 cut(s)	158, 1248, 1578, 2873
Bsh1236I	CGCG	1 cut(s)	453
BshFI	GGCC	6 cut(s)	222, 725, 1457, 1743, 2535, 3079
BshNI	GGYRCC	2 cut(s)	304, 468
BshTI	ACCGGT	1 cut(s)	626
BshVI	ATCGAT	1 cut(s)	1590
BsiHKAI	GWGCWC	5 cut(s)	88, 655, 2043, 2867, 3034
BslFI	GGGAC	5 cut(s)	733, 2480, 2787, 2977, 3086
BslI	CCNNNNNNNGG	5 cut(s)	229, 1803, 2542, 2899, 3109
BsmAI	GTCTC	6 cut(s)	29, 34, 129, 391, 1197, 2837
BsmBI	CGTCTC	1 cut(s)	34
BsmFI	GGGAC	5 cut(s)	733, 2480, 2787, 2977, 3086
BsmI	GAATGC	2 cut(s)	407, 2438
BsnI	GGCC	6 cut(s)	222, 725, 1457, 1743, 2535, 3079
Bsp1286I	GDGCHC	5 cut(s)	88, 655, 2043, 2867, 3034
Bsp13I	TCCGGA	2 cut(s)	265, 2619
Bsp1407I	TGTACA	1 cut(s)	853
Bsp19I	CCATGG	3 cut(s)	1291, 2994, 3108
BspACI	CCGC	3 cut(s)	453, 2235, 2991
BspANI	GGCC	6 cut(s)	222, 725, 1457, 1743, 2535, 3079
BspCNI	CTCAG	6 cut(s)	595, 1431, 1553, 1653, 1963, 2667
BspDI	ATCGAT	1 cut(s)	1590
BspEI	TCCGGA	2 cut(s)	265, 2619
BspFNI	CGCG	1 cut(s)	453
BspHI	TCATGA	1 cut(s)	163
BspLI	GGNNCC	6 cut(s)	306, 470, 693, 1486, 2148, 2512
BspMAI	CTGCAG	4 cut(s)	553, 2260, 2302, 2743
BspMI	ACCTGC	1 cut(s)	1270
BspOI	GCTAGC	2 cut(s)	1775, 2067
BspPI	GGATC	9 cut(s)	32, 137, 393, 812, 1045, 1088, 1479, 1492, 2677
BspQI	GCTCTTC	1 cut(s)	52
BspT107I	GGYRCC	2 cut(s)	304, 468
BspTI	CTTAAG	1 cut(s)	3073
BsrFI	RCCGGY	4 cut(s)	116, 592, 626, 2114
BsrGI	TGTACA	1 cut(s)	853
BsrI	ACTGG	2 cut(s)	1922, 2958
BssAI	RCCGGY	4 cut(s)	116, 592, 626, 2114
BssNI	GRCGYC	2 cut(s)	305, 752
BssSI	CACGAG	1 cut(s)	2860
BssT1I	CCWWGG	6 cut(s)	543, 726, 1291, 2427, 2994, 3108
Bst2BI	CACGAG	1 cut(s)	2860
Bst2UI	CCWGG	4 cut(s)	708, 748, 1679, 2151
Bst4CI	ACNGT	9 cut(s)	40, 83, 101, 1632, 1861, 2020, 2500, 2553, 3007
Bst6I	CTCTTC	4 cut(s)	52, 564, 1884, 1959
BstACI	GRCGYC	2 cut(s)	305, 752
BstAFI	CTTAAG	1 cut(s)	3073
BstAPI	GCANNNNNTGC	1 cut(s)	2111
BstAUI	TGTACA	1 cut(s)	853
BstC8I	GCNNGC	8 cut(s)	405, 594, 1282, 1334, 1741, 1773, 2001, 2065
BstDSI	CCRYGG	3 cut(s)	1291, 2994, 3108
BstEII	GGTNACC	1 cut(s)	2986
BstFNI	CGCG	1 cut(s)	453
BstH2I	RGCGCY	2 cut(s)	65, 308
BstHHI	GCGC	4 cut(s)	64, 156, 307, 453
BstMAI	GTCTC	6 cut(s)	29, 34, 129, 391, 1197, 2837
BstNI	CCWGG	4 cut(s)	708, 748, 1679, 2151
BstNSI	RCATGY	5 cut(s)	523, 1336, 1818, 2034, 2198
BstPI	GGTNACC	1 cut(s)	2986
BstSCI	CCNGG	7 cut(s)	706, 746, 873, 1677, 1743, 2149, 3015
BstSFI	CTRYAG	5 cut(s)	549, 2019, 2256, 2298, 2739
BstSLI	GKGCMC	2 cut(s)	88, 655
BstUI	CGCG	1 cut(s)	453
BstV2I	GAAGAC	4 cut(s)	613, 757, 1785, 1845
BstX2I	RGATCY	3 cut(s)	817, 1484, 2669
BstXI	CCANNNNNNTGG	3 cut(s)	776, 1685, 2953
BstYI	RGATCY	3 cut(s)	817, 1484, 2669
Bsu15I	ATCGAT	1 cut(s)	1590
BsuI	GTATCC	3 cut(s)	2397, 2711, 3023
BsuRI	GGCC	6 cut(s)	222, 725, 1457, 1743, 2535, 3079
BsuTUI	ATCGAT	1 cut(s)	1590
BtgI	CCRYGG	3 cut(s)	1291, 2994, 3108
BtgZI	GCGATG	1 cut(s)	2186
BtsI	GCAGTG	2 cut(s)	112, 2058
BtsIMutI	CAGTG	9 cut(s)	79, 106, 112, 1426, 1658, 1896, 2058, 2549, 3003
BveI	ACCTGC	1 cut(s)	1270
Cac8I	GCNNGC	8 cut(s)	405, 594, 1282, 1334, 1741, 1773, 2001, 2065
CaiI	CAGNNNCTG	1 cut(s)	1763
CciI	TCATGA	1 cut(s)	163
CfoI	GCGC	4 cut(s)	64, 156, 307, 453
Cfr10I	RCCGGY	4 cut(s)	116, 592, 626, 2114
Cfr13I	GGNCC	3 cut(s)	205, 1514, 2146
ClaI	ATCGAT	1 cut(s)	1590
Csp6I	GTAC	9 cut(s)	469, 517, 854, 960, 1315, 1399, 1950, 2744, 2882
CspAI	ACCGGT	1 cut(s)	626
CspCI	CAANNNNNGTGG	2 cut(s)	1051, 1086
CviQI	GTAC	9 cut(s)	469, 517, 854, 960, 1315, 1399, 1950, 2744, 2882
DinI	GGCGCC	1 cut(s)	306
DraIII	CACNNNGTG	1 cut(s)	1498
EaeI	YGGCCR	2 cut(s)	723, 2533
Eam1104I	CTCTTC	4 cut(s)	52, 564, 1884, 1959
EarI	CTCTTC	4 cut(s)	52, 564, 1884, 1959
Ecl136II	GAGCTC	2 cut(s)	2041, 3032
Eco130I	CCWWGG	6 cut(s)	543, 726, 1291, 2427, 2994, 3108
Eco147I	AGGCCT	2 cut(s)	1457, 3079
Eco24I	GRGCYC	2 cut(s)	2043, 3034
Eco32I	GATATC	2 cut(s)	944, 3115
Eco47I	GGWCC	3 cut(s)	205, 1514, 2146
Eco53kI	GAGCTC	2 cut(s)	2041, 3032
Eco57I	CTGAAG	5 cut(s)	1069, 1908, 1920, 1998, 3111
Eco91I	GGTNACC	1 cut(s)	2986
EcoICRI	GAGCTC	2 cut(s)	2041, 3032
EcoO109I	RGGNCCY	1 cut(s)	205
EcoO65I	GGTNACC	1 cut(s)	2986
EcoRII	CCWGG	4 cut(s)	706, 746, 1677, 2149
EcoRV	GATATC	2 cut(s)	944, 3115
EcoT14I	CCWWGG	6 cut(s)	543, 726, 1291, 2427, 2994, 3108
EcoT22I	ATGCAT	3 cut(s)	172, 907, 1820
EcoT38I	GRGCYC	2 cut(s)	2043, 3034
EgeI	GGCGCC	1 cut(s)	306
EheI	GGCGCC	1 cut(s)	306
ErhI	CCWWGG	6 cut(s)	543, 726, 1291, 2427, 2994, 3108
Esp3I	CGTCTC	1 cut(s)	34
FalI	AAGNNNNNCTT	4 cut(s)	340, 372, 749, 781
FaqI	GGGAC	5 cut(s)	733, 2480, 2787, 2977, 3086
FauNDI	CATATG	1 cut(s)	3127
FbaI	TGATCA	2 cut(s)	1435, 2181
FblI	GTMKAC	1 cut(s)	146
FriOI	GRGCYC	2 cut(s)	2043, 3034
FspBI	CTAG	7 cut(s)	341, 1377, 1772, 1947, 2064, 2273, 2456
GlaI	GCGC	4 cut(s)	63, 155, 306, 452
GsaI	CCCAGC	3 cut(s)	2141, 2255, 3107
GsuI	CTGGAG	1 cut(s)	2311
HaeII	RGCGCY	2 cut(s)	65, 308
HaeIII	GGCC	6 cut(s)	222, 725, 1457, 1743, 2535, 3079
HhaI	GCGC	4 cut(s)	64, 156, 307, 453
Hin1I	GRCGYC	2 cut(s)	305, 752
Hin6I	GCGC	4 cut(s)	62, 154, 305, 451
HinP1I	GCGC	4 cut(s)	62, 154, 305, 451
HincII	GTYRAC	2 cut(s)	147, 1754
HindII	GTYRAC	2 cut(s)	147, 1754
HindIII	AAGCTT	4 cut(s)	668, 998, 1278, 1990
HphI	GGTGA	8 cut(s)	616, 1068, 1142, 1373, 1510, 1838, 2144, 2980
Hpy166II	GTNNAC	8 cut(s)	43, 86, 147, 653, 1156, 1401, 1754, 2240
Hpy8I	GTNNAC	8 cut(s)	43, 86, 147, 653, 1156, 1401, 1754, 2240
Hpy99I	CGWCG	2 cut(s)	148, 151
HpyCH4III	ACNGT	9 cut(s)	40, 83, 101, 1632, 1861, 2020, 2500, 2553, 3007
HpyCH4IV	ACGT	1 cut(s)	752
HpySE526I	ACGT	1 cut(s)	752
Hsp92I	GRCGYC	2 cut(s)	305, 752
HspAI	GCGC	4 cut(s)	62, 154, 305, 451
KasI	GGCGCC	1 cut(s)	304
Kpn2I	TCCGGA	2 cut(s)	265, 2619
KpnI	GGTACC	1 cut(s)	472
KroI	GCCGGC	1 cut(s)	592
KroNI	GCCGGC	1 cut(s)	594
Ksp22I	TGATCA	2 cut(s)	1435, 2181
LguI	GCTCTTC	1 cut(s)	52
LmnI	GCTCC	7 cut(s)	691, 901, 1688, 1736, 2303, 3029, 3042
MaeI	CTAG	7 cut(s)	341, 1377, 1772, 1947, 2064, 2273, 2456
MaeII	ACGT	1 cut(s)	752
MfeI	CAATTG	1 cut(s)	1779
MflI	RGATCY	3 cut(s)	817, 1484, 2669
MhlI	GDGCHC	5 cut(s)	88, 655, 2043, 2867, 3034
MlsI	TGGCCA	1 cut(s)	2535
MluNI	TGGCCA	1 cut(s)	2535
Mly113I	GGCGCC	1 cut(s)	305
MlyI	GAGTC	4 cut(s)	696, 1067, 2235, 2302
MmeI	TCCRAC	5 cut(s)	166, 309, 544, 1190, 1965
Mox20I	TGGCCA	1 cut(s)	2535
Mph1103I	ATGCAT	3 cut(s)	172, 907, 1820
MroI	TCCGGA	2 cut(s)	265, 2619
MroNI	GCCGGC	1 cut(s)	592
MroXI	GAANNNNTTC	4 cut(s)	640, 1792, 1905, 2436
MscI	TGGCCA	1 cut(s)	2535
MseI	TTAA	8 cut(s)	980, 1053, 1251, 1389, 1634, 2357, 2595, 3074
MslI	CAYNNNNRTG	3 cut(s)	165, 1847, 2993
Msp20I	TGGCCA	1 cut(s)	2535
MspA1I	CMGCKG	5 cut(s)	1151, 1760, 2255, 2289, 2390
MspCI	CTTAAG	1 cut(s)	3073
MspR9I	CCNGG	7 cut(s)	708, 748, 875, 1679, 1745, 2151, 3017
MunI	CAATTG	1 cut(s)	1779
Mva1269I	GAATGC	2 cut(s)	407, 2438
MvaI	CCWGG	4 cut(s)	708, 748, 1679, 2151
MvnI	CGCG	1 cut(s)	453
NaeI	GCCGGC	1 cut(s)	594
NarI	GGCGCC	1 cut(s)	305
NciI	CCSGG	3 cut(s)	875, 1745, 3017
NcoI	CCATGG	3 cut(s)	1291, 2994, 3108
NdeI	CATATG	1 cut(s)	3127
NgoMIV	GCCGGC	1 cut(s)	592
NheI	GCTAGC	2 cut(s)	1771, 2063
NlaIV	GGNNCC	6 cut(s)	306, 470, 693, 1486, 2148, 2512
NmuCI	GTSAC	8 cut(s)	107, 787, 1379, 1424, 1826, 2009, 2056, 2986
NsiI	ATGCAT	3 cut(s)	172, 907, 1820
NspI	RCATGY	5 cut(s)	523, 1336, 1818, 2034, 2198
PaeI	GCATGC	1 cut(s)	1336
PagI	TCATGA	1 cut(s)	163
PasI	CCCWGGG	2 cut(s)	707, 2150
PceI	AGGCCT	2 cut(s)	1457, 3079
PciI	ACATGT	2 cut(s)	519, 2194
PciSI	GCTCTTC	1 cut(s)	52
PcsI	WCGNNNNNNNCGW	1 cut(s)	24
PctI	GAATGC	2 cut(s)	407, 2438
PdiI	GCCGGC	1 cut(s)	594
PdmI	GAANNNNTTC	4 cut(s)	640, 1792, 1905, 2436
PflMI	CCANNNNNTGG	2 cut(s)	2542, 2899
PfoI	TCCNGGA	1 cut(s)	1677
PinAI	ACCGGT	1 cut(s)	626
PleI	GAGTC	4 cut(s)	696, 1067, 2235, 2301
PluTI	GGCGCC	1 cut(s)	308
PpsI	GAGTC	4 cut(s)	696, 1067, 2235, 2301
PpuMI	RGGWCCY	1 cut(s)	205
PscI	ACATGT	2 cut(s)	519, 2194
PsiI	TTATAA	2 cut(s)	1988, 2337
Psp124BI	GAGCTC	2 cut(s)	2043, 3034
Psp5II	RGGWCCY	1 cut(s)	205
Psp6I	CCWGG	4 cut(s)	706, 746, 1677, 2149
PspEI	GGTNACC	1 cut(s)	2986
PspFI	CCCAGC	3 cut(s)	2137, 2251, 3103
PspGI	CCWGG	4 cut(s)	706, 746, 1677, 2149
PspN4I	GGNNCC	6 cut(s)	306, 470, 693, 1486, 2148, 2512
PspPI	GGNCC	3 cut(s)	205, 1514, 2146
PspPPI	RGGWCCY	1 cut(s)	205
PstI	CTGCAG	4 cut(s)	553, 2260, 2302, 2743
PstNI	CAGNNNCTG	1 cut(s)	1763
PsuI	RGATCY	3 cut(s)	817, 1484, 2669
PvuII	CAGCTG	5 cut(s)	1151, 1760, 2255, 2289, 2390
RsaI	GTAC	9 cut(s)	470, 518, 855, 961, 1316, 1400, 1951, 2745, 2883
RsaNI	GTAC	9 cut(s)	469, 517, 854, 960, 1315, 1399, 1950, 2744, 2882
RseI	CAYNNNNRTG	3 cut(s)	165, 1847, 2993
SacI	GAGCTC	2 cut(s)	2043, 3034
SalI	GTCGAC	1 cut(s)	145
SapI	GCTCTTC	1 cut(s)	52
SaqAI	TTAA	8 cut(s)	980, 1053, 1251, 1389, 1634, 2357, 2595, 3074
Sau96I	GGNCC	3 cut(s)	205, 1514, 2146
SbfI	CCTGCAGG	1 cut(s)	2302
ScaI	AGTACT	1 cut(s)	1951
SchI	GAGTC	4 cut(s)	696, 1067, 2235, 2302
ScrFI	CCNGG	7 cut(s)	708, 748, 875, 1679, 1745, 2151, 3017
SdaI	CCTGCAGG	1 cut(s)	2302
SduI	GDGCHC	5 cut(s)	88, 655, 2043, 2867, 3034
SfcI	CTRYAG	5 cut(s)	549, 2019, 2256, 2298, 2739
SfoI	GGCGCC	1 cut(s)	306
SgrDI	CGTCGACG	1 cut(s)	145
SinI	GGWCC	3 cut(s)	205, 1514, 2146
SmiMI	CAYNNNNRTG	3 cut(s)	165, 1847, 2993
SmlI	CTYRAG	1 cut(s)	3073
SmoI	CTYRAG	1 cut(s)	3073
SpeI	ACTAGT	2 cut(s)	1946, 2455
SphI	GCATGC	1 cut(s)	1336
Sse8387I	CCTGCAGG	1 cut(s)	2302
SseBI	AGGCCT	2 cut(s)	1457, 3079
SsiI	CCGC	3 cut(s)	453, 2235, 2991
SspDI	GGCGCC	1 cut(s)	304
SspMI	CTAG	7 cut(s)	341, 1377, 1772, 1947, 2064, 2273, 2456
SstI	GAGCTC	2 cut(s)	2043, 3034
StuI	AGGCCT	2 cut(s)	1457, 3079
StyD4I	CCNGG	7 cut(s)	706, 746, 873, 1677, 1743, 2149, 3015
StyI	CCWWGG	6 cut(s)	543, 726, 1291, 2427, 2994, 3108
TaaI	ACNGT	9 cut(s)	40, 83, 101, 1632, 1861, 2020, 2500, 2553, 3007
TaiI	ACGT	1 cut(s)	755
TaqI	TCGA	7 cut(s)	146, 441, 488, 949, 1548, 1590, 3153
TatI	WGTACW	4 cut(s)	853, 959, 1398, 1949
TauI	GCSGC	1 cut(s)	456
Tru1I	TTAA	8 cut(s)	980, 1053, 1251, 1389, 1634, 2357, 2595, 3074
Tru9I	TTAA	8 cut(s)	980, 1053, 1251, 1389, 1634, 2357, 2595, 3074
TscAI	CASTG	9 cut(s)	86, 106, 112, 1426, 1665, 1903, 2065, 2556, 3010
TseFI	GTSAC	8 cut(s)	107, 787, 1379, 1424, 1826, 2009, 2056, 2986
Tsp45I	GTSAC	8 cut(s)	107, 787, 1379, 1424, 1826, 2009, 2056, 2986
TspRI	CASTG	9 cut(s)	86, 106, 112, 1426, 1665, 1903, 2065, 2556, 3010
Van91I	CCANNNNNTGG	2 cut(s)	2542, 2899
Vha464I	CTTAAG	1 cut(s)	3073
VneI	GTGCAC	2 cut(s)	84, 651
VpaK11BI	GGWCC	3 cut(s)	205, 1514, 2146
XapI	RAATTY	4 cut(s)	1172, 1217, 2793, 2957
XceI	RCATGY	5 cut(s)	523, 1336, 1818, 2034, 2198
XmiI	GTMKAC	1 cut(s)	146
XmnI	GAANNNNTTC	4 cut(s)	640, 1792, 1905, 2436
XspI	CTAG	7 cut(s)	341, 1377, 1772, 1947, 2064, 2273, 2456
ZraI	GACGTC	1 cut(s)	753
ZrmI	AGTACT	1 cut(s)	1951
Zsp2I	ATGCAT	3 cut(s)	172, 907, 1820

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.