Alpha-aminoadipic semialdehyde
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| AlaDh_PNT_N | PF05222 | 8 - 144 | 1.4e-18 | Alanine dehydrogenase/PNT, N-terminal domain |
| Saccharop_dh_N | PF04455 | 468 - 545 | 9.5e-19 | LOR/SDH bifunctional enzyme conserved region |
| Sacchrp_dh_NADP | PF03435 | 574 - 709 | 6.9e-19 | Saccharopine dehydrogenase NADP binding domain |
| Sacchrp_dh_C | PF16653 | 713 - 1043 | 1.2e-83 | Saccharopine dehydrogenase C-terminal domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1985 |
| Acc36I | ACCTGC | 1 cut(s) | 958 |
| Acc65I | GGTACC | 1 cut(s) | 468 |
| AccB1I | GGYRCC | 1 cut(s) | 468 |
| AccI | GTMKAC | 2 cut(s) | 774, 3090 |
| AccII | CGCG | 2 cut(s) | 585, 1330 |
| AclWI | GGATC | 9 cut(s) | 137, 220, 331, 393, 770, 812, 1088, 2379, 2785 |
| AcoI | YGGCCR | 3 cut(s) | 723, 1563, 3015 |
| AcuI | CTGAAG | 4 cut(s) | 1069, 1905, 1917, 2673 |
| AcyI | GRCGYC | 2 cut(s) | 71, 3063 |
| AdeI | CACNNNGTG | 1 cut(s) | 218 |
| AfaI | GTAC | 5 cut(s) | 470, 961, 1805, 2333, 2855 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1800, 2196, 2616, 2675, 3103 |
| AflII | CTTAAG | 1 cut(s) | 2591 |
| AgeI | ACCGGT | 1 cut(s) | 626 |
| AgsI | TTSAA | 9 cut(s) | 809, 974, 1466, 1544, 1611, 1820, 1925, 2553, 2819 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1210 |
| AhlI | ACTAGT | 1 cut(s) | 791 |
| AjiI | CACGTC | 1 cut(s) | 1504 |
| AjnI | CCWGG | 1 cut(s) | 2146 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2178, 2210 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2891, 2923 |
| Alw21I | GWGCWC | 3 cut(s) | 655, 1303, 3028 |
| Alw26I | GTCTC | 4 cut(s) | 81, 360, 1197, 3064 |
| Alw44I | GTGCAC | 1 cut(s) | 651 |
| AlwI | GGATC | 9 cut(s) | 137, 220, 331, 393, 770, 812, 1088, 2379, 2785 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1438, 1760 |
| AoxI | GGCC | 5 cut(s) | 723, 1449, 1563, 1738, 3015 |
| ApaLI | GTGCAC | 1 cut(s) | 651 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2688, 2720 |
| AseI | ATTAAT | 1 cut(s) | 1839 |
| AsiGI | ACCGGT | 1 cut(s) | 626 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 640, 1323, 1902, 2433 |
| Asp718I | GGTACC | 1 cut(s) | 468 |
| AspLEI | GCGC | 4 cut(s) | 64, 86, 1133, 2813 |
| AspS9I | GGNCC | 1 cut(s) | 2143 |
| AsuC2I | CCSGG | 3 cut(s) | 1742, 2912, 3011 |
| AsuHPI | GGTGA | 5 cut(s) | 616, 1068, 1412, 1676, 2141 |
| AsuII | TTCGAA | 4 cut(s) | 1190, 1517, 1830, 2568 |
| AsuNHI | GCTAGC | 1 cut(s) | 2060 |
| AvaII | GGWCC | 1 cut(s) | 2143 |
| BaeGI | GKGCMC | 1 cut(s) | 655 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 452, 460, 485, 493 |
| BanI | GGYRCC | 1 cut(s) | 468 |
| BanII | GRGCYC | 1 cut(s) | 3028 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1219, 1251 |
| BauI | CACGAG | 1 cut(s) | 2777 |
| BbsI | GAAGAC | 3 cut(s) | 403, 607, 2822 |
| Bbv12I | GWGCWC | 3 cut(s) | 655, 1303, 3028 |
| BccI | CCATC | 9 cut(s) | 535, 601, 1122, 2128, 2158, 2251, 2357, 2830, 2909 |
| BceAI | ACGGC | 5 cut(s) | 58, 1144, 1550, 1578, 2359 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 833, 867 |
| BciT130I | CCWGG | 1 cut(s) | 2148 |
| BciVI | GTATCC | 2 cut(s) | 534, 2708 |
| BclI | TGATCA | 3 cut(s) | 1429, 1573, 2178 |
| BcnI | CCSGG | 3 cut(s) | 1742, 2912, 3011 |
| BcoDI | GTCTC | 4 cut(s) | 81, 360, 1197, 3064 |
| BcuI | ACTAGT | 1 cut(s) | 791 |
| BfaI | CTAG | 5 cut(s) | 341, 792, 1601, 2061, 2453 |
| BfmI | CTRYAG | 5 cut(s) | 137, 1470, 2016, 2253, 2295 |
| BfoI | RGCGCY | 2 cut(s) | 65, 1134 |
| BfrI | CTTAAG | 1 cut(s) | 2591 |
| BfuAI | ACCTGC | 1 cut(s) | 958 |
| BfuI | GTATCC | 2 cut(s) | 534, 2708 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 545 |
| BglII | AGATCT | 2 cut(s) | 976, 2656 |
| Bme1390I | CCNGG | 4 cut(s) | 1742, 2148, 2912, 3011 |
| Bme18I | GGWCC | 1 cut(s) | 2143 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1210 |
| BmgBI | CACGTC | 1 cut(s) | 1504 |
| BmgT120I | GGNCC | 1 cut(s) | 2143 |
| BmiI | GGNNCC | 5 cut(s) | 470, 693, 1480, 2145, 2403 |
| BmrFI | CCNGG | 4 cut(s) | 1742, 2148, 2912, 3011 |
| BmtI | GCTAGC | 1 cut(s) | 2064 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1506 |
| BpiI | GAAGAC | 3 cut(s) | 403, 607, 2822 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 342, 374, 3015, 3047 |
| BpmI | CTGGAG | 3 cut(s) | 272, 2308, 2842 |
| Bpu10I | CCTNAGC | 2 cut(s) | 457, 543 |
| Bpu14I | TTCGAA | 4 cut(s) | 1190, 1517, 1830, 2568 |
| BpuEI | CTTGAG | 1 cut(s) | 3088 |
| BpuMI | CCSGG | 3 cut(s) | 1742, 2912, 3011 |
| BsaHI | GRCGYC | 2 cut(s) | 71, 3063 |
| BsaI | GGTCTC | 1 cut(s) | 3064 |
| BsaJI | CCNNGG | 7 cut(s) | 229, 531, 726, 1291, 2146, 2147, 2988 |
| BsaWI | WCCGGW | 2 cut(s) | 116, 626 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 2724, 2754, 2891, 2921 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1800, 2196, 2616, 2675, 3103 |
| Bse118I | RCCGGY | 2 cut(s) | 626, 1996 |
| Bse1I | ACTGG | 3 cut(s) | 1948, 2094, 2952 |
| Bse3DI | GCAATG | 2 cut(s) | 177, 3108 |
| BseBI | CCWGG | 1 cut(s) | 2148 |
| BseDI | CCNNGG | 7 cut(s) | 229, 531, 726, 1291, 2146, 2147, 2988 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1800, 2196, 2616, 2675, 3103 |
| BseMI | GCAATG | 2 cut(s) | 177, 3108 |
| BseMII | CTCAG | 7 cut(s) | 24, 192, 320, 1362, 1426, 1646, 1752 |
| BseNI | ACTGG | 3 cut(s) | 1948, 2094, 2952 |
| BseRI | GAGGAG | 2 cut(s) | 1311, 2627 |
| BseSI | GKGCMC | 1 cut(s) | 655 |
| BseX3I | CGGCCG | 1 cut(s) | 1563 |
| BseYI | CCCAGC | 3 cut(s) | 1715, 2248, 3097 |
| BsgI | GTGCAG | 2 cut(s) | 1248, 1572 |
| Bsh1236I | CGCG | 2 cut(s) | 585, 1330 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1566 |
| BshFI | GGCC | 5 cut(s) | 725, 1451, 1565, 1740, 3017 |
| BshNI | GGYRCC | 1 cut(s) | 468 |
| BshTI | ACCGGT | 1 cut(s) | 626 |
| BsiEI | CGRYCG | 1 cut(s) | 1566 |
| BsiHKAI | GWGCWC | 3 cut(s) | 655, 1303, 3028 |
| BsiSI | CCGG | 8 cut(s) | 117, 266, 627, 707, 1741, 1997, 2912, 3011 |
| BslFI | GGGAC | 5 cut(s) | 1665, 2477, 2784, 2993, 3080 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1800, 2196, 2616, 2675, 3103 |
| BsmAI | GTCTC | 4 cut(s) | 81, 360, 1197, 3064 |
| BsmBI | CGTCTC | 1 cut(s) | 81 |
| BsmFI | GGGAC | 5 cut(s) | 1665, 2477, 2784, 2993, 3080 |
| BsmI | GAATGC | 2 cut(s) | 407, 2435 |
| BsnI | GGCC | 5 cut(s) | 725, 1451, 1565, 1740, 3017 |
| Bso31I | GGTCTC | 1 cut(s) | 3064 |
| Bsp119I | TTCGAA | 4 cut(s) | 1190, 1517, 1830, 2568 |
| Bsp1286I | GDGCHC | 3 cut(s) | 655, 1303, 3028 |
| Bsp19I | CCATGG | 2 cut(s) | 1291, 2988 |
| BspANI | GGCC | 5 cut(s) | 725, 1451, 1565, 1740, 3017 |
| BspCNI | CTCAG | 7 cut(s) | 25, 193, 319, 1361, 1425, 1647, 1753 |
| BspFNI | CGCG | 2 cut(s) | 585, 1330 |
| BspHI | TCATGA | 3 cut(s) | 163, 1621, 2890 |
| BspLI | GGNNCC | 5 cut(s) | 470, 693, 1480, 2145, 2403 |
| BspMAI | CTGCAG | 4 cut(s) | 141, 1474, 2257, 2299 |
| BspMI | ACCTGC | 1 cut(s) | 958 |
| BspOI | GCTAGC | 1 cut(s) | 2064 |
| BspPI | GGATC | 9 cut(s) | 137, 220, 331, 393, 770, 812, 1088, 2379, 2785 |
| BspQI | GCTCTTC | 1 cut(s) | 52 |
| BspT104I | TTCGAA | 4 cut(s) | 1190, 1517, 1830, 2568 |
| BspT107I | GGYRCC | 1 cut(s) | 468 |
| BspTI | CTTAAG | 1 cut(s) | 2591 |
| BspTNI | GGTCTC | 1 cut(s) | 3064 |
| BsrDI | GCAATG | 2 cut(s) | 177, 3108 |
| BsrFI | RCCGGY | 2 cut(s) | 626, 1996 |
| BsrI | ACTGG | 3 cut(s) | 1948, 2094, 2952 |
| BssAI | RCCGGY | 2 cut(s) | 626, 1996 |
| BssECI | CCNNGG | 7 cut(s) | 229, 531, 726, 1291, 2146, 2147, 2988 |
| BssNAI | GTATAC | 2 cut(s) | 775, 3091 |
| BssNI | GRCGYC | 2 cut(s) | 71, 3063 |
| BssSI | CACGAG | 1 cut(s) | 2777 |
| BssT1I | CCWWGG | 4 cut(s) | 531, 726, 1291, 2988 |
| Bst1107I | GTATAC | 2 cut(s) | 775, 3091 |
| Bst2BI | CACGAG | 1 cut(s) | 2777 |
| Bst2UI | CCWGG | 1 cut(s) | 2148 |
| Bst4CI | ACNGT | 8 cut(s) | 495, 779, 1679, 1858, 2017, 2231, 2497, 3001 |
| Bst6I | CTCTTC | 7 cut(s) | 52, 146, 564, 1827, 1881, 2517, 2835 |
| BstACI | GRCGYC | 2 cut(s) | 71, 3063 |
| BstAFI | CTTAAG | 1 cut(s) | 2591 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2108 |
| BstBI | TTCGAA | 4 cut(s) | 1190, 1517, 1830, 2568 |
| BstC8I | GCNNGC | 8 cut(s) | 1263, 1282, 1474, 1738, 1751, 1755, 1998, 2062 |
| BstDSI | CCRYGG | 2 cut(s) | 1291, 2988 |
| BstEII | GGTNACC | 1 cut(s) | 1418 |
| BstFNI | CGCG | 2 cut(s) | 585, 1330 |
| BstH2I | RGCGCY | 2 cut(s) | 65, 1134 |
| BstHHI | GCGC | 4 cut(s) | 64, 86, 1133, 2813 |
| BstMAI | GTCTC | 4 cut(s) | 81, 360, 1197, 3064 |
| BstMCI | CGRYCG | 1 cut(s) | 1566 |
| BstNI | CCWGG | 1 cut(s) | 2148 |
| BstNSI | RCATGY | 4 cut(s) | 322, 928, 1815, 2031 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1506 |
| BstPI | GGTNACC | 1 cut(s) | 1418 |
| BstSCI | CCNGG | 4 cut(s) | 1740, 2146, 2910, 3009 |
| BstSFI | CTRYAG | 5 cut(s) | 137, 1470, 2016, 2253, 2295 |
| BstSLI | GKGCMC | 1 cut(s) | 655 |
| BstUI | CGCG | 2 cut(s) | 585, 1330 |
| BstV2I | GAAGAC | 3 cut(s) | 403, 607, 2822 |
| BstX2I | RGATCY | 7 cut(s) | 336, 762, 817, 976, 2371, 2656, 2790 |
| BstYI | RGATCY | 7 cut(s) | 336, 762, 817, 976, 2371, 2656, 2790 |
| BstZ17I | GTATAC | 2 cut(s) | 775, 3091 |
| BstZI | CGGCCG | 1 cut(s) | 1563 |
| BsuI | GTATCC | 2 cut(s) | 534, 2708 |
| BsuRI | GGCC | 5 cut(s) | 725, 1451, 1565, 1740, 3017 |
| BtgI | CCRYGG | 2 cut(s) | 1291, 2988 |
| BtgZI | GCGATG | 1 cut(s) | 2183 |
| BtrI | CACGTC | 1 cut(s) | 1504 |
| BtsI | GCAGTG | 2 cut(s) | 79, 2055 |
| BtsIMutI | CAGTG | 6 cut(s) | 79, 1420, 1893, 1941, 2055, 2997 |
| BveI | ACCTGC | 1 cut(s) | 958 |
| Cac8I | GCNNGC | 8 cut(s) | 1263, 1282, 1474, 1738, 1751, 1755, 1998, 2062 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1438, 1760 |
| CciI | TCATGA | 3 cut(s) | 163, 1621, 2890 |
| CfoI | GCGC | 4 cut(s) | 64, 86, 1133, 2813 |
| Cfr10I | RCCGGY | 2 cut(s) | 626, 1996 |
| Cfr13I | GGNCC | 1 cut(s) | 2143 |
| CseI | GACGC | 2 cut(s) | 79, 3052 |
| Csp6I | GTAC | 5 cut(s) | 469, 960, 1804, 2332, 2854 |
| CspAI | ACCGGT | 1 cut(s) | 626 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1051, 1086 |
| CviQI | GTAC | 5 cut(s) | 469, 960, 1804, 2332, 2854 |
| DraIII | CACNNNGTG | 1 cut(s) | 218 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1210 |
| EaeI | YGGCCR | 3 cut(s) | 723, 1563, 3015 |
| EagI | CGGCCG | 1 cut(s) | 1563 |
| Eam1104I | CTCTTC | 7 cut(s) | 52, 146, 564, 1827, 1881, 2517, 2835 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1210 |
| EarI | CTCTTC | 7 cut(s) | 52, 146, 564, 1827, 1881, 2517, 2835 |
| EciI | GGCGGA | 1 cut(s) | 2974 |
| Ecl136II | GAGCTC | 1 cut(s) | 3026 |
| EclXI | CGGCCG | 1 cut(s) | 1563 |
| Eco130I | CCWWGG | 4 cut(s) | 531, 726, 1291, 2988 |
| Eco147I | AGGCCT | 1 cut(s) | 1451 |
| Eco24I | GRGCYC | 1 cut(s) | 3028 |
| Eco31I | GGTCTC | 1 cut(s) | 3064 |
| Eco32I | GATATC | 1 cut(s) | 1459 |
| Eco47I | GGWCC | 1 cut(s) | 2143 |
| Eco52I | CGGCCG | 1 cut(s) | 1563 |
| Eco53kI | GAGCTC | 1 cut(s) | 3026 |
| Eco57I | CTGAAG | 4 cut(s) | 1069, 1905, 1917, 2673 |
| Eco91I | GGTNACC | 1 cut(s) | 1418 |
| EcoICRI | GAGCTC | 1 cut(s) | 3026 |
| EcoO65I | GGTNACC | 1 cut(s) | 1418 |
| EcoRI | GAATTC | 5 cut(s) | 156, 1519, 1606, 1826, 2951 |
| EcoRII | CCWGG | 1 cut(s) | 2146 |
| EcoRV | GATATC | 1 cut(s) | 1459 |
| EcoT14I | CCWWGG | 4 cut(s) | 531, 726, 1291, 2988 |
| EcoT22I | ATGCAT | 2 cut(s) | 907, 2029 |
| EcoT38I | GRGCYC | 1 cut(s) | 3028 |
| ErhI | CCWWGG | 4 cut(s) | 531, 726, 1291, 2988 |
| Esp3I | CGTCTC | 1 cut(s) | 81 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1264, 1296 |
| FaqI | GGGAC | 5 cut(s) | 1665, 2477, 2784, 2993, 3080 |
| FauI | CCCGC | 2 cut(s) | 95, 2803 |
| FauNDI | CATATG | 1 cut(s) | 2160 |
| FbaI | TGATCA | 3 cut(s) | 1429, 1573, 2178 |
| FblI | GTMKAC | 2 cut(s) | 774, 3090 |
| FriOI | GRGCYC | 1 cut(s) | 3028 |
| FspBI | CTAG | 5 cut(s) | 341, 792, 1601, 2061, 2453 |
| GlaI | GCGC | 4 cut(s) | 63, 85, 1132, 2812 |
| GsaI | CCCAGC | 3 cut(s) | 1719, 2252, 3101 |
| GsuI | CTGGAG | 3 cut(s) | 272, 2308, 2842 |
| HaeII | RGCGCY | 2 cut(s) | 65, 1134 |
| HaeIII | GGCC | 5 cut(s) | 725, 1451, 1565, 1740, 3017 |
| HapII | CCGG | 8 cut(s) | 117, 266, 627, 707, 1741, 1997, 2912, 3011 |
| HgaI | GACGC | 2 cut(s) | 79, 3052 |
| HhaI | GCGC | 4 cut(s) | 64, 86, 1133, 2813 |
| Hin1I | GRCGYC | 2 cut(s) | 71, 3063 |
| Hin6I | GCGC | 4 cut(s) | 62, 84, 1131, 2811 |
| HinP1I | GCGC | 4 cut(s) | 62, 84, 1131, 2811 |
| HincII | GTYRAC | 1 cut(s) | 1054 |
| HindII | GTYRAC | 1 cut(s) | 1054 |
| HindIII | AAGCTT | 2 cut(s) | 668, 3117 |
| HpaII | CCGG | 8 cut(s) | 117, 266, 627, 707, 1741, 1997, 2912, 3011 |
| HphI | GGTGA | 5 cut(s) | 616, 1068, 1412, 1676, 2141 |
| Hpy166II | GTNNAC | 9 cut(s) | 43, 653, 775, 1054, 1156, 1991, 2237, 2997, 3091 |
| Hpy8I | GTNNAC | 9 cut(s) | 43, 653, 775, 1054, 1156, 1991, 2237, 2997, 3091 |
| Hpy99I | CGWCG | 1 cut(s) | 151 |
| HpyAV | CCTTC | 9 cut(s) | 304, 1892, 2092, 2194, 2362, 2420, 2428, 2444, 2516 |
| HpyCH4III | ACNGT | 8 cut(s) | 495, 779, 1679, 1858, 2017, 2231, 2497, 3001 |
| HpyCH4IV | ACGT | 7 cut(s) | 1503, 2010, 2193, 2317, 2330, 2612, 3093 |
| HpySE526I | ACGT | 7 cut(s) | 1503, 2010, 2193, 2317, 2330, 2612, 3093 |
| Hsp92I | GRCGYC | 2 cut(s) | 71, 3063 |
| HspAI | GCGC | 4 cut(s) | 62, 84, 1131, 2811 |
| KpnI | GGTACC | 1 cut(s) | 472 |
| KroI | GCCGGC | 1 cut(s) | 1996 |
| KroNI | GCCGGC | 1 cut(s) | 1998 |
| Ksp22I | TGATCA | 3 cut(s) | 1429, 1573, 2178 |
| LguI | GCTCTTC | 1 cut(s) | 52 |
| LmnI | GCTCC | 7 cut(s) | 99, 1298, 1484, 1733, 2300, 2732, 3023 |
| MaeI | CTAG | 5 cut(s) | 341, 792, 1601, 2061, 2453 |
| MaeII | ACGT | 7 cut(s) | 1503, 2010, 2193, 2317, 2330, 2612, 3093 |
| MfeI | CAATTG | 1 cut(s) | 1068 |
| MflI | RGATCY | 7 cut(s) | 336, 762, 817, 976, 2371, 2656, 2790 |
| MhlI | GDGCHC | 3 cut(s) | 655, 1303, 3028 |
| MlyI | GAGTC | 6 cut(s) | 44, 1067, 1340, 1580, 2299, 3015 |
| MmeI | TCCRAC | 4 cut(s) | 166, 845, 985, 1190 |
| Mph1103I | ATGCAT | 2 cut(s) | 907, 2029 |
| MroNI | GCCGGC | 1 cut(s) | 1996 |
| MroXI | GAANNNNTTC | 4 cut(s) | 640, 1323, 1902, 2433 |
| MslI | CAYNNNNRTG | 1 cut(s) | 165 |
| MspA1I | CMGCKG | 6 cut(s) | 102, 551, 1757, 2252, 2286, 2387 |
| MspCI | CTTAAG | 1 cut(s) | 2591 |
| MspI | CCGG | 8 cut(s) | 117, 266, 627, 707, 1741, 1997, 2912, 3011 |
| MspR9I | CCNGG | 4 cut(s) | 1742, 2148, 2912, 3011 |
| MunI | CAATTG | 1 cut(s) | 1068 |
| Mva1269I | GAATGC | 2 cut(s) | 407, 2435 |
| MvaI | CCWGG | 1 cut(s) | 2148 |
| MvnI | CGCG | 2 cut(s) | 585, 1330 |
| NaeI | GCCGGC | 1 cut(s) | 1998 |
| NciI | CCSGG | 3 cut(s) | 1742, 2912, 3011 |
| NcoI | CCATGG | 2 cut(s) | 1291, 2988 |
| NdeI | CATATG | 1 cut(s) | 2160 |
| NgoMIV | GCCGGC | 1 cut(s) | 1996 |
| NheI | GCTAGC | 1 cut(s) | 2060 |
| NlaIV | GGNNCC | 5 cut(s) | 470, 693, 1480, 2145, 2403 |
| NmeAIII | GCCGAG | 3 cut(s) | 67, 210, 432 |
| NmuCI | GTSAC | 9 cut(s) | 210, 787, 1025, 1099, 1118, 1418, 1499, 2053, 2704 |
| NsiI | ATGCAT | 2 cut(s) | 907, 2029 |
| NspI | RCATGY | 4 cut(s) | 322, 928, 1815, 2031 |
| NspV | TTCGAA | 4 cut(s) | 1190, 1517, 1830, 2568 |
| PagI | TCATGA | 3 cut(s) | 163, 1621, 2890 |
| PasI | CCCWGGG | 1 cut(s) | 2147 |
| PceI | AGGCCT | 1 cut(s) | 1451 |
| PciSI | GCTCTTC | 1 cut(s) | 52 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2314 |
| PctI | GAATGC | 2 cut(s) | 407, 2435 |
| PdiI | GCCGGC | 1 cut(s) | 1998 |
| PdmI | GAANNNNTTC | 4 cut(s) | 640, 1323, 1902, 2433 |
| PfeI | GAWTC | 9 cut(s) | 259, 600, 1008, 1366, 2075, 2122, 2381, 2570, 3126 |
| PflFI | GACNNNGTC | 1 cut(s) | 73 |
| PfoI | TCCNGGA | 1 cut(s) | 2910 |
| PinAI | ACCGGT | 1 cut(s) | 626 |
| PleI | GAGTC | 6 cut(s) | 43, 1067, 1340, 1580, 2298, 3014 |
| PpsI | GAGTC | 6 cut(s) | 43, 1067, 1340, 1580, 2298, 3014 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1506 |
| PshBI | ATTAAT | 1 cut(s) | 1839 |
| PsiI | TTATAA | 1 cut(s) | 1985 |
| Psp124BI | GAGCTC | 1 cut(s) | 3028 |
| Psp6I | CCWGG | 1 cut(s) | 2146 |
| PspEI | GGTNACC | 1 cut(s) | 1418 |
| PspFI | CCCAGC | 3 cut(s) | 1715, 2248, 3097 |
| PspGI | CCWGG | 1 cut(s) | 2146 |
| PspN4I | GGNNCC | 5 cut(s) | 470, 693, 1480, 2145, 2403 |
| PspPI | GGNCC | 1 cut(s) | 2143 |
| PstI | CTGCAG | 4 cut(s) | 141, 1474, 2257, 2299 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1438, 1760 |
| PsuI | RGATCY | 7 cut(s) | 336, 762, 817, 976, 2371, 2656, 2790 |
| PsyI | GACNNNGTC | 1 cut(s) | 73 |
| PvuII | CAGCTG | 5 cut(s) | 551, 1757, 2252, 2286, 2387 |
| RsaI | GTAC | 5 cut(s) | 470, 961, 1805, 2333, 2855 |
| RsaNI | GTAC | 5 cut(s) | 469, 960, 1804, 2332, 2854 |
| RseI | CAYNNNNRTG | 1 cut(s) | 165 |
| SacI | GAGCTC | 1 cut(s) | 3028 |
| SapI | GCTCTTC | 1 cut(s) | 52 |
| Sau96I | GGNCC | 1 cut(s) | 2143 |
| SbfI | CCTGCAGG | 1 cut(s) | 2299 |
| SchI | GAGTC | 6 cut(s) | 44, 1067, 1340, 1580, 2299, 3015 |
| ScrFI | CCNGG | 4 cut(s) | 1742, 2148, 2912, 3011 |
| SdaI | CCTGCAGG | 1 cut(s) | 2299 |
| SduI | GDGCHC | 3 cut(s) | 655, 1303, 3028 |
| SfcI | CTRYAG | 5 cut(s) | 137, 1470, 2016, 2253, 2295 |
| SfuI | TTCGAA | 4 cut(s) | 1190, 1517, 1830, 2568 |
| SinI | GGWCC | 1 cut(s) | 2143 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 165 |
| SmlI | CTYRAG | 2 cut(s) | 2591, 3067 |
| SmoI | CTYRAG | 2 cut(s) | 2591, 3067 |
| SpeI | ACTAGT | 1 cut(s) | 791 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 2299 |
| SseBI | AGGCCT | 1 cut(s) | 1451 |
| SspMI | CTAG | 5 cut(s) | 341, 792, 1601, 2061, 2453 |
| SstI | GAGCTC | 1 cut(s) | 3028 |
| StuI | AGGCCT | 1 cut(s) | 1451 |
| StyD4I | CCNGG | 4 cut(s) | 1740, 2146, 2910, 3009 |
| StyI | CCWWGG | 4 cut(s) | 531, 726, 1291, 2988 |
| TaaI | ACNGT | 8 cut(s) | 495, 779, 1679, 1858, 2017, 2231, 2497, 3001 |
| TaiI | ACGT | 7 cut(s) | 1506, 2013, 2196, 2320, 2333, 2615, 3096 |
| TauI | GCSGC | 4 cut(s) | 542, 725, 1479, 3017 |
| TfiI | GAWTC | 9 cut(s) | 259, 600, 1008, 1366, 2075, 2122, 2381, 2570, 3126 |
| TscAI | CASTG | 6 cut(s) | 86, 1420, 1900, 1948, 2062, 3004 |
| TseFI | GTSAC | 9 cut(s) | 210, 787, 1025, 1099, 1118, 1418, 1499, 2053, 2704 |
| Tsp45I | GTSAC | 9 cut(s) | 210, 787, 1025, 1099, 1118, 1418, 1499, 2053, 2704 |
| TspGWI | ACGGA | 2 cut(s) | 2256, 2879 |
| TspRI | CASTG | 6 cut(s) | 86, 1420, 1900, 1948, 2062, 3004 |
| Tth111I | GACNNNGTC | 1 cut(s) | 73 |
| Vha464I | CTTAAG | 1 cut(s) | 2591 |
| VneI | GTGCAC | 1 cut(s) | 651 |
| VpaK11BI | GGWCC | 1 cut(s) | 2143 |
| VspI | ATTAAT | 1 cut(s) | 1839 |
| XceI | RCATGY | 4 cut(s) | 322, 928, 1815, 2031 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2086 |
| XmiI | GTMKAC | 2 cut(s) | 774, 3090 |
| XmnI | GAANNNNTTC | 4 cut(s) | 640, 1323, 1902, 2433 |
| XspI | CTAG | 5 cut(s) | 341, 792, 1601, 2061, 2453 |
| Zsp2I | ATGCAT | 2 cut(s) | 907, 2029 |