RchiOBHm_Chr2g0096481

Alpha-aminoadipic semialdehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001454
Physical Location & Seq
Forward (+)
9249143 .. 9255942
6800 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47140

Sequence Viewer

Length: 3159 bp
ATGCTTGGAAATGGGGTCGTTGGGATCGTCTCGGAGACTGTGAACAAGTGGGAAAGAAGAGCGCCATTGACCCCCTCTCACTGTGCACGGCTGCTTCACAGTGGCAGTGACAAAACCGGCGTGTCTCGGATCATCGTGCAGCCGTCGACGAAGCGCATCCATCATGATGCATTGTATGAAGAAGTTGGATGTGAGATTTCAGAGGACCTGTCAGAATGTGGCCTCATTTTGGGGGTCAAACAACCCAAGCCGGAGATGATTCTTCCGGATAGAGCTTATGCTTTTTTCTCACATACCCACAAGGCGCCGAAAGAAAATATGCCATTGTTGGATAAGATTCTAGCAGAAAGGGTGTCTTTGTATGATTATGAGCTAATTGTTGGGGATCATGGGAGGAGACTGCTTGCATTCGGCAAGTTTGCTGGTAGAGCTGGATTTATCGACTTTTTGCGCGGCTTAGGACAAAGGTACCTAAGTCTTGGATATTCGACACCATTTCTTTCGTTGGGTGCTTCGTACATGTATTCATCTTTGGCTGCTGCCAAGGCTGCAGTAATTTCTGTTGGAGAAGAGATAGCAACTCAGGGATTGCCGGCAGGAATCTGCCCTCTCGTCTTTGTCTTCACCGGTTCAGGAAATGTTTCTTCTGGTGCACAAGAGATATTCAAGCTTCTCCCTCATACTTTTGTGGAGCCAAGCAGACTCCCAGGGATATTTGGGACGGCCAAGGATGCTGCTCAACCCACCAGGACGTCAAAGAGGGTCTTCCAAGTATGTGGTTGTGTTGTGACTTGTAAAGACATGGTTGAACACAAAGATCCCAAAAAATCATTTGACAAAGCTGATTATTATGTACATCCAGAACACTACAACCCGGTTTTCCATGAAAATATAGCTCCTTATGCATCTGTAATCGTGAATTGTATGTATTGGGAGAAAAGATATCCTCGATTATTGAGTACAAAGCAGTTTCAAGATTTAACAAGGAAAGGATGTAAGCTTGTTGGTATCTCTGATATAACTTGTGATATAGGGGGATCAATAGAGTTTGTTAATCAAACCACACAAATTGACTCACCTTTCTTCAGATATGATCCAATAAAAGATTCTTACCATCAGGACATGGAAGGTGATGGTGTGGTATGTTCAGCTGTGGACATTCTTCCAACAGAATTTGCAAAAGAGGCTTCCAAACACTTTGGAGACATATTATCCGAATTTGTTGGCTATTTGGCTTCTACAAAAGACATTAAAAAGTTACCTGCACACTTGATGAAAGCTTGCATAGCCCATGGAGGAACACTTACCCCATTGTACGAATACATATCTCGCATGCGAAAATCTGATGACACAGAAGAAACATCAAAAGATCATGCTAGTCACCACTTTAACAAGAAGTACACCACATTAGTATCTCTCAGTGGTCACTTGTTTGATCAATTTCTGATAAATGAGGCCTTAGATATTATTGAAGCTGCAAGTGGATCCTTCCACTTGGTGAAGTGTCAAGTTGGTCCAAGTTCTCATGCTATGTCATACTCAGAACTCGAAGTTGGTGCAGATGATGAGGAAGTTCTGAATAAAATTATCGATTCTTTGACTTCTCTTGCTAATCCAAATGAAAATCAACCGTTAAAGCAAGAAGCAAATAAGATTTCACTGAGGGTTGGTAAAGTCCAGGATAGTGGAGCTAAGAAGGAAAATGACACGAAAAAGGTCGGGATTCTAATTATTGGAGCAGGCCGGGTCTGTCAACCAGCTGCTGAAATGCTAGCATCAATTGGTGGAATGTCTTCCCAACAATGGTATAAAACATGCATGGAAGGTGACTTTGAGGAAGACATTGATGTGCAAGTTACTGTTGCATCTCTCTATCTAAAGGATGCTGAAGAGATCACTGAAGGTATTCCAAATGCAATTCCAGTTCAACTTGATGTGTCCGACACTAGTACTCTTCATAAGTATATATCTGAGGCTGAAGTTGTTATAAGCTTACTGCCTGCTTTTTGTCACATTACTGTAGCAACTGCATGTATTGAGCTCAAAAAGCACCTTGTCACTGCTAGCTATGTTGATGATGCCATGTCAGAGCTAAATGAAAAGGCAAAGACTGCCGGTATTACAATTCTTGGTGAGCTGGGCTTGGACCCTGGGATAGATCACATGATGGCGATGAAGATGATCAACCAAGCACATGTTCGTAAGGGGAAAATAAAGTCTTTTACTTCTTACTGCGGTGGACTCCCATCCCCAGCTGCAGCAAACAATCCTCTAGCATATAAGTTCAGCTGGAGTCCTGCAGGAGCTATCCGAGCTGGATGCAATCCTGCCACTTATAAATCTAATGGCGAAATCTTAAATGTTGATGGGAAGAATCTATATGATTCAGCTGTGAAACATCGGATACCTGACCTTCCAGCTTTTGCCTTGGAATGCCTTCCAAATCGTAATTCACTAGTTTATGGGGACTTGTATGGCATTGGAAAGGAAGCATCAACCGTCTTTCGTGGAACCCTTCGCTATGAAGGGTTTGGCCAAATTATGGGAACACTGTCAAGAATTGGTTTATTTGAATCCGAACCTCATCCACTTTTTAAGGATGGAAAGAAACCAACATTCCGGATGTTCTTGTCCGAACTTCTAAAAATCAAAAGCGAAGAAGTGGATGGATCTCTGAGAGGAGAGAAAGCCATCAGCGAAAGGATTATCAGTCTTGGATACAGCAAACAGCAAGAAACTGCAGTACGGGTAGCTAAAACTATCATATTTTTGGGACTGCATGAGCAGAAAGAAATTCCTGCCTCCTGCAAAAGTGCTTTTGATGTCAGTTGTCTCCTCATGGAAGATAGGTTAGCATACACGAGCACAGAACAGGATATGGTACTTCTGCACCATGAAGTGGAGGTTGAGTTCCCAGATTCTAAACTCATAGAGAAACAGAGTGCCACTTTACTGGAATTTGGGACGATGAGGAATGGTAAAATGGTCACCGCCATGGCTTTCACTGTTGGTATCCCGGCAGCCATTGGAGCTCTGCTCCTACTTGGAAACAAGATAAAAACAAGAGGTGTCTTAAGGCCTCTTGAACCTGAAGTATATGTCCCAGCCATGGATATCATACAGGCATATGGTATCAAGGTAATGGAGAAGGTCGAGTGA

Protein Analysis

1052

Amino Acids

115.57

Weight (kDa)

6.09

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AlaDh_PNT_N PF05222 8 - 143 9.8e-20 Alanine dehydrogenase/PNT, N-terminal domain
Saccharop_dh_N PF04455 470 - 540 7.2e-19 LOR/SDH bifunctional enzyme conserved region
Sacchrp_dh_NADP PF03435 575 - 710 2.2e-18 Saccharopine dehydrogenase NADP binding domain
Sacchrp_dh_C PF16653 714 - 1045 1e-79 Saccharopine dehydrogenase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1988, 2337
AatII GACGTC 1 cut(s) 755
Acc36I ACCTGC 1 cut(s) 1270
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 2 cut(s) 304, 468
AccB7I CCANNNNNTGG 2 cut(s) 2542, 2899
AccI GTMKAC 1 cut(s) 146
AccII CGCG 1 cut(s) 453
AccIII TCCGGA 2 cut(s) 265, 2619
AciI CCGC 3 cut(s) 453, 2235, 2991
AclWI GGATC 9 cut(s) 32, 137, 393, 812, 1045, 1088, 1479, 1492, 2677
AcoI YGGCCR 2 cut(s) 723, 2533
AcsI RAATTY 4 cut(s) 1172, 1217, 2793, 2957
AcuI CTGAAG 5 cut(s) 1069, 1908, 1920, 1998, 3111
AcyI GRCGYC 2 cut(s) 305, 752
AdeI CACNNNGTG 1 cut(s) 1498
AfaI GTAC 9 cut(s) 470, 518, 855, 961, 1316, 1400, 1951, 2745, 2883
AfiI CCNNNNNNNGG 5 cut(s) 229, 1803, 2542, 2899, 3109
AflII CTTAAG 1 cut(s) 3073
AflIII ACRYGT 2 cut(s) 519, 2194
AgeI ACCGGT 1 cut(s) 626
AgsI TTSAA 7 cut(s) 667, 809, 974, 1472, 1928, 2573, 3086
AhlI ACTAGT 2 cut(s) 1946, 2455
AjnI CCWGG 4 cut(s) 706, 746, 1677, 2149
AjuI GAANNNNNNNTTGG 8 cut(s) 1090, 1122, 1536, 1568, 2181, 2213, 2434, 2466
AloI GAACNNNNNNTCC 2 cut(s) 2894, 2926
Alw21I GWGCWC 5 cut(s) 88, 655, 2043, 2867, 3034
Alw26I GTCTC 6 cut(s) 29, 34, 129, 391, 1197, 2837
Alw44I GTGCAC 2 cut(s) 84, 651
AlwI GGATC 9 cut(s) 32, 137, 393, 812, 1045, 1088, 1479, 1492, 2677
AlwNI CAGNNNCTG 1 cut(s) 1763
Aor13HI TCCGGA 2 cut(s) 265, 2619
AoxI GGCC 6 cut(s) 220, 723, 1455, 1741, 2533, 3077
ApaLI GTGCAC 2 cut(s) 84, 651
ApoI RAATTY 4 cut(s) 1172, 1217, 2793, 2957
AsiGI ACCGGT 1 cut(s) 626
Asp700I GAANNNNTTC 4 cut(s) 640, 1792, 1905, 2436
Asp718I GGTACC 1 cut(s) 468
AspLEI GCGC 4 cut(s) 64, 156, 307, 453
AspS9I GGNCC 3 cut(s) 205, 1514, 2146
AsuC2I CCSGG 3 cut(s) 875, 1745, 3017
AsuHPI GGTGA 8 cut(s) 616, 1068, 1142, 1373, 1510, 1838, 2144, 2980
AsuNHI GCTAGC 2 cut(s) 1771, 2063
AvaII GGWCC 3 cut(s) 205, 1514, 2146
BaeGI GKGCMC 2 cut(s) 88, 655
BaeI ACNNNNGTAYC 6 cut(s) 452, 460, 485, 493, 1395, 1428
BalI TGGCCA 1 cut(s) 2535
BamHI GGATCC 1 cut(s) 1484
BanI GGYRCC 2 cut(s) 304, 468
BanII GRGCYC 2 cut(s) 2043, 3034
BauI CACGAG 1 cut(s) 2860
BbsI GAAGAC 4 cut(s) 613, 757, 1785, 1845
Bbv12I GWGCWC 5 cut(s) 88, 655, 2043, 2867, 3034
BccI CCATC 9 cut(s) 168, 1122, 1127, 2161, 2254, 2360, 2594, 2660, 2699
BceAI ACGGC 3 cut(s) 104, 127, 738
BcgI CGANNNNNNTGC 4 cut(s) 1538, 1572, 2301, 2335
BciT130I CCWGG 4 cut(s) 708, 748, 1679, 2151
BciVI GTATCC 3 cut(s) 2397, 2711, 3023
BclI TGATCA 2 cut(s) 1435, 2181
BcnI CCSGG 3 cut(s) 875, 1745, 3017
BcoDI GTCTC 6 cut(s) 29, 34, 129, 391, 1197, 2837
BcuI ACTAGT 2 cut(s) 1946, 2455
BfaI CTAG 7 cut(s) 341, 1377, 1772, 1947, 2064, 2273, 2456
BfmI CTRYAG 5 cut(s) 549, 2019, 2256, 2298, 2739
BfoI RGCGCY 2 cut(s) 65, 308
BfrI CTTAAG 1 cut(s) 3073
BfuAI ACCTGC 1 cut(s) 1270
BfuI GTATCC 3 cut(s) 2397, 2711, 3023
BmcAI AGTACT 1 cut(s) 1951
Bme1390I CCNGG 7 cut(s) 708, 748, 875, 1679, 1745, 2151, 3017
Bme18I GGWCC 3 cut(s) 205, 1514, 2146
BmgT120I GGNCC 3 cut(s) 205, 1514, 2146
BmiI GGNNCC 6 cut(s) 306, 470, 693, 1486, 2148, 2512
BmrFI CCNGG 7 cut(s) 708, 748, 875, 1679, 1745, 2151, 3017
BmtI GCTAGC 2 cut(s) 1775, 2067
BpiI GAAGAC 4 cut(s) 613, 757, 1785, 1845
BplI GAGNNNNNCTC 2 cut(s) 3021, 3053
BpmI CTGGAG 1 cut(s) 2311
Bpu10I CCTNAGC 1 cut(s) 457
BpuMI CCSGG 3 cut(s) 875, 1745, 3017
Bsa29I ATCGAT 1 cut(s) 1590
BsaHI GRCGYC 2 cut(s) 305, 752
BsaWI WCCGGW 3 cut(s) 265, 626, 2619
BsaXI ACNNNNNCTCC 2 cut(s) 2894, 2924
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 1803, 2542, 2899, 3109
Bse118I RCCGGY 4 cut(s) 116, 592, 626, 2114
Bse1I ACTGG 2 cut(s) 1922, 2958
BseAI TCCGGA 2 cut(s) 265, 2619
BseBI CCWGG 4 cut(s) 708, 748, 1679, 2151
BseCI ATCGAT 1 cut(s) 1590
BseLI CCNNNNNNNGG 5 cut(s) 229, 1803, 2542, 2899, 3109
BseMII CTCAG 6 cut(s) 596, 1432, 1554, 1652, 1962, 2666
BseNI ACTGG 2 cut(s) 1922, 2958
BseRI GAGGAG 3 cut(s) 409, 2694, 2825
BseSI GKGCMC 2 cut(s) 88, 655
BseYI CCCAGC 3 cut(s) 2137, 2251, 3103
BsgI GTGCAG 4 cut(s) 158, 1248, 1578, 2873
Bsh1236I CGCG 1 cut(s) 453
BshFI GGCC 6 cut(s) 222, 725, 1457, 1743, 2535, 3079
BshNI GGYRCC 2 cut(s) 304, 468
BshTI ACCGGT 1 cut(s) 626
BshVI ATCGAT 1 cut(s) 1590
BsiHKAI GWGCWC 5 cut(s) 88, 655, 2043, 2867, 3034
BslFI GGGAC 5 cut(s) 733, 2480, 2787, 2977, 3086
BslI CCNNNNNNNGG 5 cut(s) 229, 1803, 2542, 2899, 3109
BsmAI GTCTC 6 cut(s) 29, 34, 129, 391, 1197, 2837
BsmBI CGTCTC 1 cut(s) 34
BsmFI GGGAC 5 cut(s) 733, 2480, 2787, 2977, 3086
BsmI GAATGC 2 cut(s) 407, 2438
BsnI GGCC 6 cut(s) 222, 725, 1457, 1743, 2535, 3079
Bsp1286I GDGCHC 5 cut(s) 88, 655, 2043, 2867, 3034
Bsp13I TCCGGA 2 cut(s) 265, 2619
Bsp1407I TGTACA 1 cut(s) 853
Bsp19I CCATGG 3 cut(s) 1291, 2994, 3108
BspACI CCGC 3 cut(s) 453, 2235, 2991
BspANI GGCC 6 cut(s) 222, 725, 1457, 1743, 2535, 3079
BspCNI CTCAG 6 cut(s) 595, 1431, 1553, 1653, 1963, 2667
BspDI ATCGAT 1 cut(s) 1590
BspEI TCCGGA 2 cut(s) 265, 2619
BspFNI CGCG 1 cut(s) 453
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 6 cut(s) 306, 470, 693, 1486, 2148, 2512
BspMAI CTGCAG 4 cut(s) 553, 2260, 2302, 2743
BspMI ACCTGC 1 cut(s) 1270
BspOI GCTAGC 2 cut(s) 1775, 2067
BspPI GGATC 9 cut(s) 32, 137, 393, 812, 1045, 1088, 1479, 1492, 2677
BspQI GCTCTTC 1 cut(s) 52
BspT107I GGYRCC 2 cut(s) 304, 468
BspTI CTTAAG 1 cut(s) 3073
BsrFI RCCGGY 4 cut(s) 116, 592, 626, 2114
BsrGI TGTACA 1 cut(s) 853
BsrI ACTGG 2 cut(s) 1922, 2958
BssAI RCCGGY 4 cut(s) 116, 592, 626, 2114
BssNI GRCGYC 2 cut(s) 305, 752
BssSI CACGAG 1 cut(s) 2860
BssT1I CCWWGG 6 cut(s) 543, 726, 1291, 2427, 2994, 3108
Bst2BI CACGAG 1 cut(s) 2860
Bst2UI CCWGG 4 cut(s) 708, 748, 1679, 2151
Bst4CI ACNGT 9 cut(s) 40, 83, 101, 1632, 1861, 2020, 2500, 2553, 3007
Bst6I CTCTTC 4 cut(s) 52, 564, 1884, 1959
BstACI GRCGYC 2 cut(s) 305, 752
BstAFI CTTAAG 1 cut(s) 3073
BstAPI GCANNNNNTGC 1 cut(s) 2111
BstAUI TGTACA 1 cut(s) 853
BstC8I GCNNGC 8 cut(s) 405, 594, 1282, 1334, 1741, 1773, 2001, 2065
BstDSI CCRYGG 3 cut(s) 1291, 2994, 3108
BstEII GGTNACC 1 cut(s) 2986
BstFNI CGCG 1 cut(s) 453
BstH2I RGCGCY 2 cut(s) 65, 308
BstHHI GCGC 4 cut(s) 64, 156, 307, 453
BstMAI GTCTC 6 cut(s) 29, 34, 129, 391, 1197, 2837
BstNI CCWGG 4 cut(s) 708, 748, 1679, 2151
BstNSI RCATGY 5 cut(s) 523, 1336, 1818, 2034, 2198
BstPI GGTNACC 1 cut(s) 2986
BstSCI CCNGG 7 cut(s) 706, 746, 873, 1677, 1743, 2149, 3015
BstSFI CTRYAG 5 cut(s) 549, 2019, 2256, 2298, 2739
BstSLI GKGCMC 2 cut(s) 88, 655
BstUI CGCG 1 cut(s) 453
BstV2I GAAGAC 4 cut(s) 613, 757, 1785, 1845
BstX2I RGATCY 3 cut(s) 817, 1484, 2669
BstXI CCANNNNNNTGG 3 cut(s) 776, 1685, 2953
BstYI RGATCY 3 cut(s) 817, 1484, 2669
Bsu15I ATCGAT 1 cut(s) 1590
BsuI GTATCC 3 cut(s) 2397, 2711, 3023
BsuRI GGCC 6 cut(s) 222, 725, 1457, 1743, 2535, 3079
BsuTUI ATCGAT 1 cut(s) 1590
BtgI CCRYGG 3 cut(s) 1291, 2994, 3108
BtgZI GCGATG 1 cut(s) 2186
BtsI GCAGTG 2 cut(s) 112, 2058
BtsIMutI CAGTG 9 cut(s) 79, 106, 112, 1426, 1658, 1896, 2058, 2549, 3003
BveI ACCTGC 1 cut(s) 1270
Cac8I GCNNGC 8 cut(s) 405, 594, 1282, 1334, 1741, 1773, 2001, 2065
CaiI CAGNNNCTG 1 cut(s) 1763
CciI TCATGA 1 cut(s) 163
CfoI GCGC 4 cut(s) 64, 156, 307, 453
Cfr10I RCCGGY 4 cut(s) 116, 592, 626, 2114
Cfr13I GGNCC 3 cut(s) 205, 1514, 2146
ClaI ATCGAT 1 cut(s) 1590
Csp6I GTAC 9 cut(s) 469, 517, 854, 960, 1315, 1399, 1950, 2744, 2882
CspAI ACCGGT 1 cut(s) 626
CspCI CAANNNNNGTGG 2 cut(s) 1051, 1086
CviQI GTAC 9 cut(s) 469, 517, 854, 960, 1315, 1399, 1950, 2744, 2882
DinI GGCGCC 1 cut(s) 306
DraIII CACNNNGTG 1 cut(s) 1498
EaeI YGGCCR 2 cut(s) 723, 2533
Eam1104I CTCTTC 4 cut(s) 52, 564, 1884, 1959
EarI CTCTTC 4 cut(s) 52, 564, 1884, 1959
Ecl136II GAGCTC 2 cut(s) 2041, 3032
Eco130I CCWWGG 6 cut(s) 543, 726, 1291, 2427, 2994, 3108
Eco147I AGGCCT 2 cut(s) 1457, 3079
Eco24I GRGCYC 2 cut(s) 2043, 3034
Eco32I GATATC 2 cut(s) 944, 3115
Eco47I GGWCC 3 cut(s) 205, 1514, 2146
Eco53kI GAGCTC 2 cut(s) 2041, 3032
Eco57I CTGAAG 5 cut(s) 1069, 1908, 1920, 1998, 3111
Eco91I GGTNACC 1 cut(s) 2986
EcoICRI GAGCTC 2 cut(s) 2041, 3032
EcoO109I RGGNCCY 1 cut(s) 205
EcoO65I GGTNACC 1 cut(s) 2986
EcoRII CCWGG 4 cut(s) 706, 746, 1677, 2149
EcoRV GATATC 2 cut(s) 944, 3115
EcoT14I CCWWGG 6 cut(s) 543, 726, 1291, 2427, 2994, 3108
EcoT22I ATGCAT 3 cut(s) 172, 907, 1820
EcoT38I GRGCYC 2 cut(s) 2043, 3034
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
ErhI CCWWGG 6 cut(s) 543, 726, 1291, 2427, 2994, 3108
Esp3I CGTCTC 1 cut(s) 34
FalI AAGNNNNNCTT 4 cut(s) 340, 372, 749, 781
FaqI GGGAC 5 cut(s) 733, 2480, 2787, 2977, 3086
FauNDI CATATG 1 cut(s) 3127
FbaI TGATCA 2 cut(s) 1435, 2181
FblI GTMKAC 1 cut(s) 146
FriOI GRGCYC 2 cut(s) 2043, 3034
FspBI CTAG 7 cut(s) 341, 1377, 1772, 1947, 2064, 2273, 2456
GlaI GCGC 4 cut(s) 63, 155, 306, 452
GsaI CCCAGC 3 cut(s) 2141, 2255, 3107
GsuI CTGGAG 1 cut(s) 2311
HaeII RGCGCY 2 cut(s) 65, 308
HaeIII GGCC 6 cut(s) 222, 725, 1457, 1743, 2535, 3079
HhaI GCGC 4 cut(s) 64, 156, 307, 453
Hin1I GRCGYC 2 cut(s) 305, 752
Hin6I GCGC 4 cut(s) 62, 154, 305, 451
HinP1I GCGC 4 cut(s) 62, 154, 305, 451
HincII GTYRAC 2 cut(s) 147, 1754
HindII GTYRAC 2 cut(s) 147, 1754
HindIII AAGCTT 4 cut(s) 668, 998, 1278, 1990
HphI GGTGA 8 cut(s) 616, 1068, 1142, 1373, 1510, 1838, 2144, 2980
Hpy166II GTNNAC 8 cut(s) 43, 86, 147, 653, 1156, 1401, 1754, 2240
Hpy8I GTNNAC 8 cut(s) 43, 86, 147, 653, 1156, 1401, 1754, 2240
Hpy99I CGWCG 2 cut(s) 148, 151
HpyCH4III ACNGT 9 cut(s) 40, 83, 101, 1632, 1861, 2020, 2500, 2553, 3007
HpyCH4IV ACGT 1 cut(s) 752
HpySE526I ACGT 1 cut(s) 752
Hsp92I GRCGYC 2 cut(s) 305, 752
HspAI GCGC 4 cut(s) 62, 154, 305, 451
KasI GGCGCC 1 cut(s) 304
Kpn2I TCCGGA 2 cut(s) 265, 2619
KpnI GGTACC 1 cut(s) 472
KroI GCCGGC 1 cut(s) 592
KroNI GCCGGC 1 cut(s) 594
Ksp22I TGATCA 2 cut(s) 1435, 2181
LguI GCTCTTC 1 cut(s) 52
LmnI GCTCC 7 cut(s) 691, 901, 1688, 1736, 2303, 3029, 3042
MaeI CTAG 7 cut(s) 341, 1377, 1772, 1947, 2064, 2273, 2456
MaeII ACGT 1 cut(s) 752
MfeI CAATTG 1 cut(s) 1779
MflI RGATCY 3 cut(s) 817, 1484, 2669
MhlI GDGCHC 5 cut(s) 88, 655, 2043, 2867, 3034
MlsI TGGCCA 1 cut(s) 2535
MluNI TGGCCA 1 cut(s) 2535
Mly113I GGCGCC 1 cut(s) 305
MlyI GAGTC 4 cut(s) 696, 1067, 2235, 2302
MmeI TCCRAC 5 cut(s) 166, 309, 544, 1190, 1965
Mox20I TGGCCA 1 cut(s) 2535
Mph1103I ATGCAT 3 cut(s) 172, 907, 1820
MroI TCCGGA 2 cut(s) 265, 2619
MroNI GCCGGC 1 cut(s) 592
MroXI GAANNNNTTC 4 cut(s) 640, 1792, 1905, 2436
MscI TGGCCA 1 cut(s) 2535
MseI TTAA 8 cut(s) 980, 1053, 1251, 1389, 1634, 2357, 2595, 3074
MslI CAYNNNNRTG 3 cut(s) 165, 1847, 2993
Msp20I TGGCCA 1 cut(s) 2535
MspA1I CMGCKG 5 cut(s) 1151, 1760, 2255, 2289, 2390
MspCI CTTAAG 1 cut(s) 3073
MspR9I CCNGG 7 cut(s) 708, 748, 875, 1679, 1745, 2151, 3017
MunI CAATTG 1 cut(s) 1779
Mva1269I GAATGC 2 cut(s) 407, 2438
MvaI CCWGG 4 cut(s) 708, 748, 1679, 2151
MvnI CGCG 1 cut(s) 453
NaeI GCCGGC 1 cut(s) 594
NarI GGCGCC 1 cut(s) 305
NciI CCSGG 3 cut(s) 875, 1745, 3017
NcoI CCATGG 3 cut(s) 1291, 2994, 3108
NdeI CATATG 1 cut(s) 3127
NgoMIV GCCGGC 1 cut(s) 592
NheI GCTAGC 2 cut(s) 1771, 2063
NlaIV GGNNCC 6 cut(s) 306, 470, 693, 1486, 2148, 2512
NmuCI GTSAC 8 cut(s) 107, 787, 1379, 1424, 1826, 2009, 2056, 2986
NsiI ATGCAT 3 cut(s) 172, 907, 1820
NspI RCATGY 5 cut(s) 523, 1336, 1818, 2034, 2198
PaeI GCATGC 1 cut(s) 1336
PagI TCATGA 1 cut(s) 163
PasI CCCWGGG 2 cut(s) 707, 2150
PceI AGGCCT 2 cut(s) 1457, 3079
PciI ACATGT 2 cut(s) 519, 2194
PciSI GCTCTTC 1 cut(s) 52
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 2 cut(s) 407, 2438
PdiI GCCGGC 1 cut(s) 594
PdmI GAANNNNTTC 4 cut(s) 640, 1792, 1905, 2436
PflMI CCANNNNNTGG 2 cut(s) 2542, 2899
PfoI TCCNGGA 1 cut(s) 1677
PinAI ACCGGT 1 cut(s) 626
PleI GAGTC 4 cut(s) 696, 1067, 2235, 2301
PluTI GGCGCC 1 cut(s) 308
PpsI GAGTC 4 cut(s) 696, 1067, 2235, 2301
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 2 cut(s) 519, 2194
PsiI TTATAA 2 cut(s) 1988, 2337
Psp124BI GAGCTC 2 cut(s) 2043, 3034
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 4 cut(s) 706, 746, 1677, 2149
PspEI GGTNACC 1 cut(s) 2986
PspFI CCCAGC 3 cut(s) 2137, 2251, 3103
PspGI CCWGG 4 cut(s) 706, 746, 1677, 2149
PspN4I GGNNCC 6 cut(s) 306, 470, 693, 1486, 2148, 2512
PspPI GGNCC 3 cut(s) 205, 1514, 2146
PspPPI RGGWCCY 1 cut(s) 205
PstI CTGCAG 4 cut(s) 553, 2260, 2302, 2743
PstNI CAGNNNCTG 1 cut(s) 1763
PsuI RGATCY 3 cut(s) 817, 1484, 2669
PvuII CAGCTG 5 cut(s) 1151, 1760, 2255, 2289, 2390
RsaI GTAC 9 cut(s) 470, 518, 855, 961, 1316, 1400, 1951, 2745, 2883
RsaNI GTAC 9 cut(s) 469, 517, 854, 960, 1315, 1399, 1950, 2744, 2882
RseI CAYNNNNRTG 3 cut(s) 165, 1847, 2993
SacI GAGCTC 2 cut(s) 2043, 3034
SalI GTCGAC 1 cut(s) 145
SapI GCTCTTC 1 cut(s) 52
SaqAI TTAA 8 cut(s) 980, 1053, 1251, 1389, 1634, 2357, 2595, 3074
Sau96I GGNCC 3 cut(s) 205, 1514, 2146
SbfI CCTGCAGG 1 cut(s) 2302
ScaI AGTACT 1 cut(s) 1951
SchI GAGTC 4 cut(s) 696, 1067, 2235, 2302
ScrFI CCNGG 7 cut(s) 708, 748, 875, 1679, 1745, 2151, 3017
SdaI CCTGCAGG 1 cut(s) 2302
SduI GDGCHC 5 cut(s) 88, 655, 2043, 2867, 3034
SfcI CTRYAG 5 cut(s) 549, 2019, 2256, 2298, 2739
SfoI GGCGCC 1 cut(s) 306
SgrDI CGTCGACG 1 cut(s) 145
SinI GGWCC 3 cut(s) 205, 1514, 2146
SmiMI CAYNNNNRTG 3 cut(s) 165, 1847, 2993
SmlI CTYRAG 1 cut(s) 3073
SmoI CTYRAG 1 cut(s) 3073
SpeI ACTAGT 2 cut(s) 1946, 2455
SphI GCATGC 1 cut(s) 1336
Sse8387I CCTGCAGG 1 cut(s) 2302
SseBI AGGCCT 2 cut(s) 1457, 3079
SsiI CCGC 3 cut(s) 453, 2235, 2991
SspDI GGCGCC 1 cut(s) 304
SspMI CTAG 7 cut(s) 341, 1377, 1772, 1947, 2064, 2273, 2456
SstI GAGCTC 2 cut(s) 2043, 3034
StuI AGGCCT 2 cut(s) 1457, 3079
StyD4I CCNGG 7 cut(s) 706, 746, 873, 1677, 1743, 2149, 3015
StyI CCWWGG 6 cut(s) 543, 726, 1291, 2427, 2994, 3108
TaaI ACNGT 9 cut(s) 40, 83, 101, 1632, 1861, 2020, 2500, 2553, 3007
TaiI ACGT 1 cut(s) 755
TaqI TCGA 7 cut(s) 146, 441, 488, 949, 1548, 1590, 3153
TatI WGTACW 4 cut(s) 853, 959, 1398, 1949
TauI GCSGC 1 cut(s) 456
Tru1I TTAA 8 cut(s) 980, 1053, 1251, 1389, 1634, 2357, 2595, 3074
Tru9I TTAA 8 cut(s) 980, 1053, 1251, 1389, 1634, 2357, 2595, 3074
TscAI CASTG 9 cut(s) 86, 106, 112, 1426, 1665, 1903, 2065, 2556, 3010
TseFI GTSAC 8 cut(s) 107, 787, 1379, 1424, 1826, 2009, 2056, 2986
Tsp45I GTSAC 8 cut(s) 107, 787, 1379, 1424, 1826, 2009, 2056, 2986
TspRI CASTG 9 cut(s) 86, 106, 112, 1426, 1665, 1903, 2065, 2556, 3010
Van91I CCANNNNNTGG 2 cut(s) 2542, 2899
Vha464I CTTAAG 1 cut(s) 3073
VneI GTGCAC 2 cut(s) 84, 651
VpaK11BI GGWCC 3 cut(s) 205, 1514, 2146
XapI RAATTY 4 cut(s) 1172, 1217, 2793, 2957
XceI RCATGY 5 cut(s) 523, 1336, 1818, 2034, 2198
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 4 cut(s) 640, 1792, 1905, 2436
XspI CTAG 7 cut(s) 341, 1377, 1772, 1947, 2064, 2273, 2456
ZraI GACGTC 1 cut(s) 753
ZrmI AGTACT 1 cut(s) 1951
Zsp2I ATGCAT 3 cut(s) 172, 907, 1820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.