AT4G33500

Stage II sporulation protein E (SpoIIE)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004725
Physical Location & Seq
Reverse (-)
16112624 .. 16116383
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33500.1

Sequence Viewer

Length: 2175 bp
ATGGCCGATCATTTGATTCTTTCTCTTCAAGCTCCTCCTTTTCTCATCTTCCCTTGTTCTCTTCACCGTTCATGGAGATTTCCCGGTGGAATCAGATATTCCGTTCCTGAGTTCCGATTGTCCTCTCAGCTACAGTTGGCTAATTCCATTTCACCTTCAAAGTCTTCAGCTTCCTCTTCTTCTCCACCGGAGAATTCTGCTCCAGAGAAGTTCGATCTCGTGTCCAGCACTCAGCTTAAAGATGGAAGTCATGTCTTTCGGTTCGGGGATGCAAGTGAGATTGAGAAATATCTCGAGGCTGAAGAAAAAGCGAGATGTGTTGAGGTAGAAACGCAAAATGCTAAGATTGCTGAAGAAGCCAGTGAAGTTTCAAGGAAACAAAAGAAATTGGTCAGTTCTATTATTGAAACAAGTACTGAGAAAGAGGAAACAGCTGCTCCTTCCGATCTTAGCAATGTCATTAAAATAAAGGATCGTAAAAGGGTTCGTTCTCCTACTAAGAAGAAGAAAGAAACTGTGAATGTATCACGGAGCGAAGATAAAATTGATGCCAAGAGTGCTAGTGTTTCCAATCTGAGCTCCATAGTTAGTGTAGCAGAAGCTATTCCAATTAGCTCTACTGAAGAAGAAGCTGTTGTTGAGAAGGAAATCACGGCTAAGTCATATAATGTAGAGCCTCTTTCTTCTGAAGCCATGAAGAAAGTTTCTGTCAATAAAATAGGAGACTGTGAGACAAATGGTTATCAAGAGAATCGTATGGAGGTTCAGGCAAGACCGTCATTGTCTACACAACAGGAAATTACGCCTGTATCCACCATTGAAATTGATGACAATTTGGATGTGACGGAAAAGCCTATTGAAGCTGAGGAGAATCTTGTCGCGGAACCAACAGCTACAGATGATTTGTCTCCTGATGAACTCCTTTCAACTTCAGAAGCAACACATCGCAGCGTTGATGAGATTGCTCAAAAACCTGTAATTGATACGTCTGAGGAAAACCTTCTGAACACTTTTGAAGCTGAGGAGAATCCTGTAGTTGAACCAACAGCTACAGCTGCTGTCTCTTCTGATGAACTCATTTCAACTTCAGAAGCAACACGTCACAGCGTTGATGAGATCGCTCAAAAGCCTATTATTGATACGTCTGAGAAAAACCCTATGGAAACTTTTGTTGAACCAGAAGCAGTGCATAGTAGCGTTGACGAGAGCACTGAAAAGCTTGTAGTTGTTACGTCTGATGTGGAAAATGATGGAGAAAATGTAGCTTCAACCACAGAAGATGAAATAACTGTGAGGGACACAATAACTGATAGTGGTAGCATATCCAATAATGATGACACAAAAGTGGAAGATCTCCAACTTCCTGTACCTGAAACGGCCAGTCTTGAACCCATTAAAGCGGCTTCTGGCAGGGAAGAACTTGTGTCCAAAGCATTTTACTTGGACTCTGGTTTTGCTTCACTCCAAAGCCCCTTTAAGGCATTGGCAGGTCGGGAAGATGCTTATTTTATCTCTCACCACAATTGGATAGGTATTGCTGATGGAGTCTCTCAGTGGTCATTTGAAGGAATCAATAAGGGAATGTATGCTCAAGAACTCATGAGCAACTGTGAAAAGATTATTTCCAATGAAACTGCTAAGATAAGTGACCCAGTTCAAGTTCTCCATAGAAGTGTCAATGAGACTAAGTCCTCTGGATCATCAACAGCCTTAATTGCTCATCTTGACAATAATGAACTCCACATAGCCAATATTGGAGATTCGGGATTCATGGTCATTAGGGATGGCACAGTATTGCAGAATTCGTCTCCAATGTTTCATCATTTTTGTTTTCCCTTGCATATTACGCAAGGCTGTGATGTCCTAAAACTTGCAGAGGTTTACCATGTGAACCTGGAAGAAGGCGATGTTGTCATCGCAGCAACTGATGGGCTCTTTGATAACTTATACGAAAAAGAAATCGTTTCAATAGTTTGCGGGTCACTGAAACAGAGTCTGGAACCTCAGAAAATAGCAGAGTTGGTAGCTGCAAAAGCACAAGAAGTAGGACGATCTAAGACAGAGAGAACGCCATTTGCTGACGCAGCTAAAGAAGAAGGATACAATGGACACAAAGGTGGTAAACTTGATGCTGTTACCGTCATCATTTCATTCGTGAAAATTGTCTCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C SpoIIE

Protein Analysis

724

Amino Acids

78.92

Weight (kDa)

4.66

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 526 - 683 6.3e-06 Stage II sporulation protein E (SpoIIE)
PP2C_2 PF13672 559 - 668 6.3e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1478
AccI GTMKAC 1 cut(s) 785
AccII CGCG 1 cut(s) 881
AciI CCGC 3 cut(s) 881, 1400, 1977
AclWI GGATC 2 cut(s) 480, 1705
AcoI YGGCCR 2 cut(s) 3, 1377
AcsI RAATTY 2 cut(s) 193, 1801
AcuI CTGAAG 7 cut(s) 150, 321, 372, 642, 708, 915, 1071
AfaI GTAC 2 cut(s) 415, 1368
AfiI CCNNNNNNNGG 1 cut(s) 1477
AflIII ACRYGT 1 cut(s) 1097
AjiI CACGTC 1 cut(s) 1100
AjnI CCWGG 1 cut(s) 1893
AleI CACNNNNGTG 2 cut(s) 1343, 2115
Alw21I GWGCWC 2 cut(s) 581, 1211
Alw26I GTCTC 8 cut(s) 717, 725, 912, 1066, 1552, 1676, 1812, 2170
AlwI GGATC 2 cut(s) 480, 1705
AlwNI CAGNNNCTG 3 cut(s) 1058, 1925, 1996
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 2 cut(s) 3, 1377
ApeKI GCWGC 6 cut(s) 434, 948, 1055, 1919, 2027, 2084
ApoI RAATTY 2 cut(s) 193, 1801
ArsI GACNNNNNNTTYG 2 cut(s) 1436, 1468
Asp700I GAANNNNTTC 2 cut(s) 603, 999
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 56, 144, 1508
AvaI CYCGRG 1 cut(s) 293
BaeI ACNNNNGTAYC 2 cut(s) 792, 825
BanII GRGCYC 2 cut(s) 581, 1935
BauI CACGAG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 581, 1211
BbvCI CCTCAGC 2 cut(s) 864, 1020
BbvI GCAGC 6 cut(s) 421, 960, 1042, 1931, 2014, 2096
BccI CCATC 5 cut(s) 236, 1244, 1535, 1778, 1922
BceAI ACGGC 2 cut(s) 669, 1392
BciT130I CCWGG 1 cut(s) 1895
BciVI GTATCC 2 cut(s) 820, 2093
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 8 cut(s) 717, 725, 912, 1066, 1552, 1676, 1812, 2170
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 4 cut(s) 131, 894, 1032, 1050
BfuAI ACCTGC 1 cut(s) 1478
BfuI GTATCC 2 cut(s) 820, 2093
BglII AGATCT 1 cut(s) 1351
BisI GCNGC 7 cut(s) 435, 949, 1056, 1401, 1920, 2028, 2085
BlsI GCNGC 7 cut(s) 436, 950, 1057, 1402, 1921, 2029, 2086
BmcAI AGTACT 1 cut(s) 415
Bme1390I CCNGG 2 cut(s) 84, 1895
BmeT110I CYCGRG 1 cut(s) 293
BmgBI CACGTC 1 cut(s) 1100
BmiI GGNNCC 2 cut(s) 885, 2001
BmrFI CCNGG 2 cut(s) 84, 1895
BmrI ACTGGG 1 cut(s) 1646
BmsI GCATC 4 cut(s) 259, 538, 1489, 2119
BmuI ACTGGG 1 cut(s) 1646
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 1 cut(s) 186
Bpu10I CCTNAGC 2 cut(s) 864, 1020
BpuEI CTTGAG 1 cut(s) 1575
BpuMI CCSGG 1 cut(s) 84
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 4 cut(s) 421, 451, 1245, 1275
Bsc4I CCNNNNNNNGG 1 cut(s) 1477
Bse1I ACTGG 3 cut(s) 360, 1380, 1652
Bse3DI GCAATG 1 cut(s) 460
BseBI CCWGG 1 cut(s) 1895
BseGI GGATG 3 cut(s) 274, 844, 1789
BseLI CCNNNNNNNGG 1 cut(s) 1477
BseMI GCAATG 1 cut(s) 460
BseNI ACTGG 3 cut(s) 360, 1380, 1652
BseRI GAGGAG 3 cut(s) 24, 881, 1037
BseXI GCAGC 6 cut(s) 421, 960, 1042, 1931, 2014, 2096
Bsh1236I CGCG 1 cut(s) 881
BshFI GGCC 2 cut(s) 5, 1379
BsiHKAI GWGCWC 2 cut(s) 581, 1211
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 2 cut(s) 84, 188
BslFI GGGAC 1 cut(s) 1310
BslI CCNNNNNNNGG 1 cut(s) 1477
BsmAI GTCTC 8 cut(s) 717, 725, 912, 1066, 1552, 1676, 1812, 2170
BsmBI CGTCTC 1 cut(s) 1812
BsmFI GGGAC 1 cut(s) 1310
BsnI GGCC 2 cut(s) 5, 1379
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 3 cut(s) 581, 1211, 1935
Bsp143I GATC 8 cut(s) 7, 214, 445, 472, 1116, 1351, 1697, 2051
BspACI CCGC 3 cut(s) 881, 1400, 1977
BspANI GGCC 2 cut(s) 5, 1379
BspFNI CGCG 1 cut(s) 881
BspHI TCATGA 1 cut(s) 1599
BspLI GGNNCC 2 cut(s) 885, 2001
BspMI ACCTGC 1 cut(s) 1478
BspPI GGATC 2 cut(s) 480, 1705
BsrDI GCAATG 1 cut(s) 460
BsrI ACTGG 3 cut(s) 360, 1380, 1652
BssMI GATC 8 cut(s) 7, 214, 445, 472, 1116, 1351, 1697, 2051
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 1895
Bst4CI ACNGT 9 cut(s) 68, 135, 517, 728, 777, 1291, 1610, 1792, 2140
Bst6I CTCTTC 4 cut(s) 30, 66, 181, 1069
BstF5I GGATG 3 cut(s) 274, 844, 1789
BstFNI CGCG 1 cut(s) 881
BstKTI GATC 8 cut(s) 10, 217, 448, 475, 1119, 1354, 1700, 2054
BstMAI GTCTC 8 cut(s) 717, 725, 912, 1066, 1552, 1676, 1812, 2170
BstMBI GATC 8 cut(s) 7, 214, 445, 472, 1116, 1351, 1697, 2051
BstMWI GCNNNNNNNGC 8 cut(s) 347, 356, 557, 1055, 1715, 1846, 2033, 2084
BstNI CCWGG 1 cut(s) 1895
BstSCI CCNGG 2 cut(s) 82, 1893
BstSFI CTRYAG 4 cut(s) 131, 894, 1032, 1050
BstUI CGCG 1 cut(s) 881
BstV1I GCAGC 6 cut(s) 421, 960, 1042, 1931, 2014, 2096
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 1351
BstYI RGATCY 1 cut(s) 1351
BsuI GTATCC 2 cut(s) 820, 2093
BsuRI GGCC 2 cut(s) 5, 1379
BtgZI GCGATG 3 cut(s) 929, 1900, 1920
BtrI CACGTC 1 cut(s) 1100
BtsCI GGATG 3 cut(s) 274, 844, 1789
BtsI GCAGTG 1 cut(s) 1191
BtsIMutI CAGTG 5 cut(s) 367, 1191, 1209, 1559, 1982
BveI ACCTGC 1 cut(s) 1478
CaiI CAGNNNCTG 3 cut(s) 1058, 1925, 1996
CciI TCATGA 1 cut(s) 1599
CseI GACGC 1 cut(s) 2090
Csp6I GTAC 2 cut(s) 414, 1367
CviAII CATG 6 cut(s) 72, 251, 694, 1600, 1771, 1886
CviQI GTAC 2 cut(s) 414, 1367
DpnI GATC 8 cut(s) 9, 216, 447, 474, 1118, 1353, 1699, 2053
DpnII GATC 8 cut(s) 7, 214, 445, 472, 1116, 1351, 1697, 2051
EaeI YGGCCR 2 cut(s) 3, 1377
Eam1104I CTCTTC 4 cut(s) 30, 66, 181, 1069
EarI CTCTTC 4 cut(s) 30, 66, 181, 1069
Ecl136II GAGCTC 1 cut(s) 579
Eco24I GRGCYC 2 cut(s) 581, 1935
Eco53kI GAGCTC 1 cut(s) 579
Eco57I CTGAAG 7 cut(s) 150, 321, 372, 642, 708, 915, 1071
Eco88I CYCGRG 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 579
EcoRI GAATTC 2 cut(s) 193, 1801
EcoRII CCWGG 1 cut(s) 1893
EcoT38I GRGCYC 2 cut(s) 581, 1935
Esp3I CGTCTC 1 cut(s) 1812
FaeI CATG 6 cut(s) 75, 254, 697, 1603, 1774, 1889
FaqI GGGAC 1 cut(s) 1310
FatI CATG 6 cut(s) 71, 250, 693, 1599, 1770, 1885
FauI CCCGC 1 cut(s) 1970
FblI GTMKAC 1 cut(s) 785
Fnu4HI GCNGC 7 cut(s) 435, 949, 1056, 1401, 1920, 2028, 2085
FokI GGATG 3 cut(s) 281, 851, 1796
FriOI GRGCYC 2 cut(s) 581, 1935
Fsp4HI GCNGC 7 cut(s) 435, 949, 1056, 1401, 1920, 2028, 2085
FspBI CTAG 1 cut(s) 561
GluI GCNGC 7 cut(s) 435, 949, 1056, 1401, 1920, 2028, 2085
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 2 cut(s) 5, 1379
HapII CCGG 2 cut(s) 84, 188
HgaI GACGC 1 cut(s) 2090
Hin1II CATG 6 cut(s) 75, 254, 697, 1603, 1774, 1889
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HindIII AAGCTT 1 cut(s) 1217
HpaII CCGG 2 cut(s) 84, 188
HphI GGTGA 3 cut(s) 56, 144, 1508
Hpy166II GTNNAC 5 cut(s) 786, 1201, 1882, 1891, 2123
Hpy8I GTNNAC 5 cut(s) 786, 1201, 1882, 1891, 2123
HpyAV CCTTC 7 cut(s) 165, 450, 637, 1010, 1559, 1895, 2090
HpyCH4III ACNGT 9 cut(s) 68, 135, 517, 728, 777, 1291, 1610, 1792, 2140
HpyCH4IV ACGT 4 cut(s) 986, 1099, 1142, 1232
HpyCH4V TGCA 6 cut(s) 272, 1189, 1798, 1840, 1874, 2030
HpyF10VI GCNNNNNNNGC 8 cut(s) 347, 356, 557, 1055, 1715, 1846, 2033, 2084
HpySE526I ACGT 4 cut(s) 986, 1099, 1142, 1232
Hsp92II CATG 6 cut(s) 75, 254, 697, 1603, 1774, 1889
Kzo9I GATC 8 cut(s) 7, 214, 445, 472, 1116, 1351, 1697, 2051
LmnI GCTCC 5 cut(s) 37, 205, 442, 531, 584
Lsp1109I GCAGC 6 cut(s) 421, 960, 1042, 1931, 2014, 2096
LweI GCATC 4 cut(s) 259, 538, 1489, 2119
MaeI CTAG 1 cut(s) 561
MaeII ACGT 4 cut(s) 986, 1099, 1142, 1232
MaeIII GTNAC 6 cut(s) 841, 1100, 1228, 1646, 1980, 2134
MalI GATC 8 cut(s) 9, 216, 447, 474, 1118, 1353, 1699, 2053
MboI GATC 8 cut(s) 7, 214, 445, 472, 1116, 1351, 1697, 2051
MfeI CAATTG 1 cut(s) 1522
MflI RGATCY 1 cut(s) 1351
MhlI GDGCHC 3 cut(s) 581, 1211, 1935
MlyI GAGTC 3 cut(s) 1439, 1554, 2002
MmeI TCCRAC 1 cut(s) 1381
MroXI GAANNNNTTC 2 cut(s) 603, 999
MseI TTAA 5 cut(s) 237, 462, 1395, 1476, 1712
MslI CAYNNNNRTG 3 cut(s) 1343, 1671, 2115
MspA1I CMGCKG 2 cut(s) 434, 1055
MspI CCGG 2 cut(s) 84, 188
MspR9I CCNGG 2 cut(s) 84, 1895
MunI CAATTG 1 cut(s) 1522
MvaI CCWGG 1 cut(s) 1895
MvnI CGCG 1 cut(s) 881
MwoI GCNNNNNNNGC 8 cut(s) 347, 356, 557, 1055, 1715, 1846, 2033, 2084
NciI CCSGG 1 cut(s) 84
NdeII GATC 8 cut(s) 7, 214, 445, 472, 1116, 1351, 1697, 2051
NlaIII CATG 6 cut(s) 75, 254, 697, 1603, 1774, 1889
NlaIV GGNNCC 2 cut(s) 885, 2001
NmuCI GTSAC 4 cut(s) 841, 1100, 1646, 1980
OliI CACNNNNGTG 2 cut(s) 1343, 2115
PaeR7I CTCGAG 1 cut(s) 293
PagI TCATGA 1 cut(s) 1599
PdmI GAANNNNTTC 2 cut(s) 603, 999
PfeI GAWTC 8 cut(s) 16, 90, 751, 871, 1027, 1569, 1760, 1767
PflFI GACNNNGTC 1 cut(s) 1687
PkrI GCNGC 7 cut(s) 436, 950, 1057, 1402, 1921, 2029, 2086
PleI GAGTC 3 cut(s) 1439, 1553, 2001
PpsI GAGTC 3 cut(s) 1439, 1553, 2001
Psp124BI GAGCTC 1 cut(s) 581
Psp6I CCWGG 1 cut(s) 1893
PspGI CCWGG 1 cut(s) 1893
PspN4I GGNNCC 2 cut(s) 885, 2001
PstNI CAGNNNCTG 3 cut(s) 1058, 1925, 1996
PsuI RGATCY 1 cut(s) 1351
PsyI GACNNNGTC 1 cut(s) 1687
PvuII CAGCTG 2 cut(s) 434, 1055
RsaI GTAC 2 cut(s) 415, 1368
RsaNI GTAC 2 cut(s) 414, 1367
RseI CAYNNNNRTG 3 cut(s) 1343, 1671, 2115
SacI GAGCTC 1 cut(s) 581
SaqAI TTAA 5 cut(s) 237, 462, 1395, 1476, 1712
SatI GCNGC 7 cut(s) 435, 949, 1056, 1401, 1920, 2028, 2085
Sau3AI GATC 8 cut(s) 7, 214, 445, 472, 1116, 1351, 1697, 2051
ScaI AGTACT 1 cut(s) 415
SchI GAGTC 3 cut(s) 1439, 1554, 2002
ScrFI CCNGG 2 cut(s) 84, 1895
SduI GDGCHC 3 cut(s) 581, 1211, 1935
SfaNI GCATC 4 cut(s) 259, 538, 1489, 2119
SfcI CTRYAG 4 cut(s) 131, 894, 1032, 1050
Sfr274I CTCGAG 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 293
SmiMI CAYNNNNRTG 3 cut(s) 1343, 1671, 2115
SmlI CTYRAG 2 cut(s) 293, 1590
SmoI CTYRAG 2 cut(s) 293, 1590
SsiI CCGC 3 cut(s) 881, 1400, 1977
SspI AATATT 1 cut(s) 1753
SspMI CTAG 1 cut(s) 561
SstI GAGCTC 1 cut(s) 581
StyD4I CCNGG 2 cut(s) 82, 1893
TaaI ACNGT 9 cut(s) 68, 135, 517, 728, 777, 1291, 1610, 1792, 2140
TaiI ACGT 4 cut(s) 989, 1102, 1145, 1235
TaqI TCGA 2 cut(s) 213, 294
TatI WGTACW 1 cut(s) 413
TauI GCSGC 1 cut(s) 1403
TfiI GAWTC 8 cut(s) 16, 90, 751, 871, 1027, 1569, 1760, 1767
Tru1I TTAA 5 cut(s) 237, 462, 1395, 1476, 1712
Tru9I TTAA 5 cut(s) 237, 462, 1395, 1476, 1712
TscAI CASTG 5 cut(s) 367, 1191, 1216, 1559, 1989
TseFI GTSAC 4 cut(s) 841, 1100, 1646, 1980
TseI GCWGC 6 cut(s) 434, 948, 1055, 1919, 2027, 2084
Tsp45I GTSAC 4 cut(s) 841, 1100, 1646, 1980
TspGWI ACGGA 3 cut(s) 91, 544, 860
TspRI CASTG 5 cut(s) 367, 1191, 1216, 1559, 1989
Tth111I GACNNNGTC 1 cut(s) 1687
XapI RAATTY 2 cut(s) 193, 1801
XhoI CTCGAG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 785
XmnI GAANNNNTTC 2 cut(s) 603, 999
XspI CTAG 1 cut(s) 561
ZrmI AGTACT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.