Rorug07G0235800

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004725
Physical Location & Seq
Reverse (-)
21636699 .. 21639280
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0235800.1

Sequence Viewer

Length: 1575 bp
ATGGGTCTAAAACTCAAGGAAAACCCACCATCCCCAGCCTTCATAGAAACAGTGGAAGAGATCATGAAAATCTACAAGTCACTGCCACCAAGGCCTTCCATTGAAGAGGTTGAAGCAGCCATGTCTGTGCTCAAAACAGTCGACACTGAAGAAGAACAGAGACTTGAGGAGATATCAAAGCAGGTGGTCCCCAAAGATGTCCCTGAAGAACTCTTCTCTGTTTTGCTGCAGGTGAGGAGGACCATGGTCCTGTTTCAGAGCCATGAACAGAGGAGAGAGGCTTCTGAGGTGGTTGAGTTTGACAAGATGTTTCAGACTTTTGATGGCCTTATTCAGAGGACTTCTGCGCTGGTTTCTGGTGACACCCAGAAGGAGAATCATGTTGCTTTTGGGTACCCAGTTGGGGAAATTGTGAGTGATCCTGGGATTAGTGATGACAGTCTTGTGGTGATGAAGAAGAAGAAGAAGAACAAGGAAGATGAAGAGTTGAAGATAGATGGTTTCAAGGGTTTGGTGAAGAGTTCTTCCACTATTTCTTCAGGAGAGTCTGAGAAATTAAGTCTAATGAAGGTTGCAGCTATAATAGAAAACACTGCTAAAAGTGGAGAAGTAGTTCTTGATCTTAGAGGCAAATTAGTAGATCAAGTGGAGTGGCTTCCAGTATCACTTGGGAAATTATCAGGTGTCACTGAGTTGGACTTGTCCGAAAACCGAATCATGGCTATTCCGCCCACCATTGGTGGCCTTAAAGCATTAACAAAGGTTGACTTCCACTCAAACCAACTCATAAACCTTCCAGACTCATTTGGTGAGTTGAGTAATCTCACTGATCTTGATCTCCATGCAAACAGATTGAAATCGCTTCCTGCTTCATTCGGAAACTTGATCAACCTCATGAATCTTGATTTGAGTTCAAATAGCTTCACCCGTTTGCCTGATGTAATTGGGAAGCTGATCAGCTTGAAGACATTGAATGCTGAAACAAATGATCTTGAGGAACTGCCTTTCACAATCGGATCCTGCACATCACTCGCTGAGCTGCATTTGAATTTCAATCAGCTAAGAGCTCTGCCCGAGGCAATTGGAAAGCTCGAAAACTTGGAGATCCTCACAGTTCACTACAACAGAATCAAAGGGTTGCCAACAACAATGGGGAATCTTAGCAGTTTGAAGGAGCTTGATGTTAGCTTCAACGAACTCGAATCCATACCCGAGAACTTGTGTTTTGCAGTGCGCCTCGAAAAACTGAATGTGTCAAACAACTTTGCTGACTTGACAGCTTTGCCGCGGTCAATTGGAAATCTTGAGATGCTTGAAGAGTTGGATATCAGTGATGATCAAATTAGGCAATTACCACTCTCTTTCAGGTTCTTGTCAAAACTGAAGGTTTTCCGTGCCAATGAAACACCTTTGGAAGTTCCCCCTAGACATGTTATCCAGTTGGGTCCTCAGGCAGTTGTAAAGTACATGGAAGATTATGTTGCCAAGATGGATGCTGCATTTCAACCATTGAAGAAGAAGAAGAAGGGGTTTTGGTTTTGGTTTTGCTCAAAACTCTGCTCCACCAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C SpoIIE

Protein Analysis

524

Amino Acids

58.61

Weight (kDa)

5.26

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 228 - 285 2e-08 Leucine rich repeat
LRR_14 PF23598 261 - 326 3.6e-10 Leucine-rich repeat region
LRR_8 PF13855 273 - 331 1.8e-07 Leucine rich repeat
LRR_14 PF23598 315 - 418 4.6e-12 Leucine-rich repeat region
LRR_8 PF13855 343 - 400 1.6e-08 Leucine rich repeat
LRR_14 PF23598 424 - 468 2.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 172, 220
Acc36I ACCTGC 2 cut(s) 172, 220
Acc65I GGTACC 1 cut(s) 393
AccB1I GGYRCC 1 cut(s) 393
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 1288
AciI CCGC 3 cut(s) 728, 1286, 1288
AclWI GGATC 4 cut(s) 413, 1011, 1024, 1099
AcsI RAATTY 1 cut(s) 1048
AcuI CTGAAG 4 cut(s) 168, 225, 522, 1403
AfaI GTAC 2 cut(s) 395, 1466
AfiI CCNNNNNNNGG 3 cut(s) 403, 718, 737
AflIII ACRYGT 1 cut(s) 1429
AjnI CCWGG 1 cut(s) 421
AloI GAACNNNNNNTCC 2 cut(s) 597, 629
Alw21I GWGCWC 2 cut(s) 132, 1069
Alw26I GTCTC 1 cut(s) 154
AlwI GGATC 4 cut(s) 413, 1011, 1024, 1099
Ama87I CYCGRG 2 cut(s) 1073, 1211
AoxI GGCC 3 cut(s) 92, 325, 742
ApeKI GCWGC 5 cut(s) 116, 226, 575, 1039, 1496
ApoI RAATTY 1 cut(s) 1048
Asp700I GAANNNNTTC 2 cut(s) 612, 1388
Asp718I GGTACC 1 cut(s) 393
AspLEI GCGC 2 cut(s) 349, 1236
AspS9I GGNCC 4 cut(s) 187, 240, 247, 1445
AsuHPI GGTGA 6 cut(s) 244, 371, 460, 526, 821, 916
AvaI CYCGRG 2 cut(s) 1073, 1211
AvaII GGWCC 4 cut(s) 187, 240, 247, 1445
AxyI CCTNAGG 1 cut(s) 1449
BamHI GGATCC 1 cut(s) 1016
BanI GGYRCC 1 cut(s) 393
BanII GRGCYC 1 cut(s) 1069
BbsI GAAGAC 1 cut(s) 971
Bbv12I GWGCWC 2 cut(s) 132, 1069
BbvI GCAGC 5 cut(s) 128, 213, 587, 1026, 1483
BccI CCATC 4 cut(s) 37, 317, 491, 1483
BcgI CGANNNNNNTGC 2 cut(s) 1012, 1046
BciT130I CCWGG 1 cut(s) 423
BclI TGATCA 3 cut(s) 885, 954, 1336
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 2 cut(s) 1425, 1573
BfmI CTRYAG 1 cut(s) 227
BfuAI ACCTGC 2 cut(s) 172, 220
BglI GCCNNNNNGGC 1 cut(s) 91
BisI GCNGC 6 cut(s) 117, 227, 576, 1040, 1286, 1497
BlpI GCTNAGC 1 cut(s) 1035
BlsI GCNGC 6 cut(s) 118, 228, 577, 1041, 1287, 1498
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 4 cut(s) 187, 240, 247, 1445
BmeT110I CYCGRG 2 cut(s) 1073, 1211
BmgT120I GGNCC 4 cut(s) 187, 240, 247, 1445
BmiI GGNNCC 4 cut(s) 189, 395, 1018, 1446
BmrFI CCNGG 1 cut(s) 423
BmrI ACTGGG 1 cut(s) 392
BmsI GCATC 2 cut(s) 1299, 1483
BmuI ACTGGG 1 cut(s) 392
BoxI GACNNNNGTC 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 971
Bpu1102I GCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 3 cut(s) 185, 1013, 1325
BsaBI GATNNNNATC 2 cut(s) 834, 856
BsaJI CCNNGG 5 cut(s) 89, 243, 422, 1074, 1286
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 3 cut(s) 403, 718, 737
Bse1I ACTGG 3 cut(s) 398, 659, 1438
Bse21I CCTNAGG 1 cut(s) 1449
Bse8I GATNNNNATC 2 cut(s) 834, 856
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 5 cut(s) 89, 243, 422, 1074, 1286
BseGI GGATG 2 cut(s) 29, 1498
BseJI GATNNNNATC 2 cut(s) 834, 856
BseLI CCNNNNNNNGG 3 cut(s) 403, 718, 737
BseMII CTCAG 5 cut(s) 276, 540, 681, 1026, 1463
BseNI ACTGG 3 cut(s) 398, 659, 1438
BseRI GAGGAG 3 cut(s) 182, 250, 286
BseXI GCAGC 5 cut(s) 128, 213, 587, 1026, 1483
BseYI CCCAGC 1 cut(s) 34
BsgI GTGCAG 1 cut(s) 1006
Bsh1236I CGCG 1 cut(s) 1288
BshFI GGCC 3 cut(s) 94, 327, 744
BshNI GGYRCC 1 cut(s) 393
BsiHKAI GWGCWC 2 cut(s) 132, 1069
BsiHKCI CYCGRG 2 cut(s) 1073, 1211
BslFI GGGAC 2 cut(s) 173, 185
BslI CCNNNNNNNGG 3 cut(s) 403, 718, 737
BsmAI GTCTC 1 cut(s) 154
BsmFI GGGAC 2 cut(s) 173, 185
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 3 cut(s) 94, 327, 744
BsoBI CYCGRG 2 cut(s) 1073, 1211
Bsp1286I GDGCHC 2 cut(s) 132, 1069
Bsp1720I GCTNAGC 1 cut(s) 1035
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 3 cut(s) 728, 1286, 1288
BspANI GGCC 3 cut(s) 94, 327, 744
BspCNI CTCAG 5 cut(s) 277, 541, 682, 1027, 1462
BspFNI CGCG 1 cut(s) 1288
BspHI TCATGA 2 cut(s) 63, 894
BspLI GGNNCC 4 cut(s) 189, 395, 1018, 1446
BspMAI CTGCAG 1 cut(s) 231
BspMI ACCTGC 2 cut(s) 172, 220
BspPI GGATC 4 cut(s) 413, 1011, 1024, 1099
BspT107I GGYRCC 1 cut(s) 393
BsrI ACTGG 3 cut(s) 398, 659, 1438
BssECI CCNNGG 5 cut(s) 89, 243, 422, 1074, 1286
BssT1I CCWWGG 2 cut(s) 89, 243
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 4 cut(s) 52, 139, 440, 1114
Bst6I CTCTTC 6 cut(s) 51, 99, 218, 477, 512, 1311
BstDEI CTNAG 8 cut(s) 285, 549, 623, 690, 1035, 1061, 1160, 1449
BstDSI CCRYGG 2 cut(s) 243, 1286
BstF5I GGATG 2 cut(s) 29, 1498
BstFNI CGCG 1 cut(s) 1288
BstHHI GCGC 2 cut(s) 349, 1236
BstMAI GTCTC 1 cut(s) 154
BstMWI GCNNNNNNNGC 1 cut(s) 91
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 1 cut(s) 1433
BstPAI GACNNNNGTC 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 227
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 5 cut(s) 128, 213, 587, 1026, 1483
BstV2I GAAGAC 1 cut(s) 971
BstX2I RGATCY 2 cut(s) 1016, 1104
BstYI RGATCY 2 cut(s) 1016, 1104
Bsu36I CCTNAGG 1 cut(s) 1449
BsuRI GGCC 3 cut(s) 94, 327, 744
BtgI CCRYGG 2 cut(s) 243, 1286
BtsCI GGATG 2 cut(s) 29, 1498
BtsI GCAGTG 3 cut(s) 80, 591, 1236
BtsIMutI CAGTG 8 cut(s) 57, 80, 144, 591, 687, 825, 1236, 1336
BveI ACCTGC 2 cut(s) 172, 220
CciI TCATGA 2 cut(s) 63, 894
CfoI GCGC 2 cut(s) 349, 1236
Cfr13I GGNCC 4 cut(s) 187, 240, 247, 1445
Cfr42I CCGCGG 1 cut(s) 1289
Csp6I GTAC 2 cut(s) 394, 1465
CspCI CAANNNNNGTGG 4 cut(s) 165, 200, 632, 667
CviQI GTAC 2 cut(s) 394, 1465
DdeI CTNAG 8 cut(s) 285, 549, 623, 690, 1035, 1061, 1160, 1449
Eam1104I CTCTTC 6 cut(s) 51, 99, 218, 477, 512, 1311
EarI CTCTTC 6 cut(s) 51, 99, 218, 477, 512, 1311
EciI GGCGGA 1 cut(s) 717
Ecl136II GAGCTC 1 cut(s) 1067
Eco130I CCWWGG 2 cut(s) 89, 243
Eco147I AGGCCT 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 1069
Eco32I GATATC 2 cut(s) 174, 1327
Eco47I GGWCC 4 cut(s) 187, 240, 247, 1445
Eco53kI GAGCTC 1 cut(s) 1067
Eco57I CTGAAG 4 cut(s) 168, 225, 522, 1403
Eco81I CCTNAGG 1 cut(s) 1449
Eco88I CYCGRG 2 cut(s) 1073, 1211
EcoICRI GAGCTC 1 cut(s) 1067
EcoO109I RGGNCCY 1 cut(s) 1445
EcoRII CCWGG 1 cut(s) 421
EcoRV GATATC 2 cut(s) 174, 1327
EcoT14I CCWWGG 2 cut(s) 89, 243
EcoT38I GRGCYC 1 cut(s) 1069
ErhI CCWWGG 2 cut(s) 89, 243
FalI AAGNNNNNCTT 4 cut(s) 600, 632, 752, 784
FaqI GGGAC 2 cut(s) 173, 185
FbaI TGATCA 3 cut(s) 885, 954, 1336
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 6 cut(s) 117, 227, 576, 1040, 1286, 1497
FokI GGATG 2 cut(s) 16, 1505
FriOI GRGCYC 1 cut(s) 1069
Fsp4HI GCNGC 6 cut(s) 117, 227, 576, 1040, 1286, 1497
FspBI CTAG 2 cut(s) 1425, 1573
GlaI GCGC 2 cut(s) 348, 1235
GluI GCNGC 6 cut(s) 117, 227, 576, 1040, 1286, 1497
GsaI CCCAGC 1 cut(s) 38
HaeIII GGCC 3 cut(s) 94, 327, 744
HhaI GCGC 2 cut(s) 349, 1236
Hin6I GCGC 2 cut(s) 347, 1234
HinP1I GCGC 2 cut(s) 347, 1234
HincII GTYRAC 2 cut(s) 142, 766
HindII GTYRAC 2 cut(s) 142, 766
HinfI GANTC 8 cut(s) 376, 545, 714, 800, 898, 1128, 1156, 1202
HphI GGTGA 6 cut(s) 244, 371, 460, 526, 821, 916
Hpy166II GTNNAC 3 cut(s) 142, 766, 1117
Hpy188I TCNGA 8 cut(s) 258, 286, 315, 336, 550, 706, 878, 1016
Hpy188III TCNNGA 9 cut(s) 64, 540, 617, 797, 833, 895, 902, 992, 1304
Hpy8I GTNNAC 3 cut(s) 142, 766, 1117
HpyAV CCTTC 8 cut(s) 49, 105, 364, 562, 803, 1165, 1378, 1519
HpyCH4III ACNGT 4 cut(s) 52, 139, 440, 1114
HpyCH4V TGCA 7 cut(s) 229, 575, 845, 1023, 1042, 1229, 1499
HpyF10VI GCNNNNNNNGC 1 cut(s) 91
HpyF3I CTNAG 8 cut(s) 285, 549, 623, 690, 1035, 1061, 1160, 1449
HspAI GCGC 2 cut(s) 347, 1234
KpnI GGTACC 1 cut(s) 397
Ksp22I TGATCA 3 cut(s) 885, 954, 1336
KspI CCGCGG 1 cut(s) 1289
LmnI GCTCC 2 cut(s) 1174, 1565
Lsp1109I GCAGC 5 cut(s) 128, 213, 587, 1026, 1483
LweI GCATC 2 cut(s) 1299, 1483
MaeI CTAG 2 cut(s) 1425, 1573
MaeIII GTNAC 3 cut(s) 78, 359, 685
MfeI CAATTG 2 cut(s) 1080, 1293
MflI RGATCY 2 cut(s) 1016, 1104
MhlI GDGCHC 2 cut(s) 132, 1069
MlyI GAGTC 2 cut(s) 554, 794
MmeI TCCRAC 2 cut(s) 675, 1302
MroXI GAANNNNTTC 2 cut(s) 612, 1388
MseI TTAA 3 cut(s) 557, 747, 755
MslI CAYNNNNRTG 1 cut(s) 125
MspA1I CMGCKG 1 cut(s) 1288
MspR9I CCNGG 1 cut(s) 423
MunI CAATTG 2 cut(s) 1080, 1293
Mva1269I GAATGC 1 cut(s) 979
MvaI CCWGG 1 cut(s) 423
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 1 cut(s) 91
NcoI CCATGG 1 cut(s) 243
NlaIV GGNNCC 4 cut(s) 189, 395, 1018, 1446
NmuCI GTSAC 3 cut(s) 78, 359, 685
NspI RCATGY 1 cut(s) 1433
PagI TCATGA 2 cut(s) 63, 894
PaqCI CACCTGC 2 cut(s) 172, 220
PceI AGGCCT 1 cut(s) 94
PciI ACATGT 1 cut(s) 1429
PctI GAATGC 1 cut(s) 979
PdmI GAANNNNTTC 2 cut(s) 612, 1388
PfeI GAWTC 6 cut(s) 376, 714, 898, 1128, 1156, 1202
PkrI GCNGC 6 cut(s) 118, 228, 577, 1041, 1287, 1498
PleI GAGTC 2 cut(s) 553, 794
PpsI GAGTC 2 cut(s) 553, 794
PpuMI RGGWCCY 1 cut(s) 1445
PscI ACATGT 1 cut(s) 1429
PshAI GACNNNNGTC 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 1069
Psp5II RGGWCCY 1 cut(s) 1445
Psp6I CCWGG 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 4 cut(s) 189, 395, 1018, 1446
PspPI GGNCC 4 cut(s) 187, 240, 247, 1445
PspPPI RGGWCCY 1 cut(s) 1445
PstI CTGCAG 1 cut(s) 231
PsuI RGATCY 2 cut(s) 1016, 1104
RsaI GTAC 2 cut(s) 395, 1466
RsaNI GTAC 2 cut(s) 394, 1465
RseI CAYNNNNRTG 1 cut(s) 125
SacI GAGCTC 1 cut(s) 1069
SacII CCGCGG 1 cut(s) 1289
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 3 cut(s) 557, 747, 755
SatI GCNGC 6 cut(s) 117, 227, 576, 1040, 1286, 1497
Sau96I GGNCC 4 cut(s) 187, 240, 247, 1445
SchI GAGTC 2 cut(s) 554, 794
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 2 cut(s) 132, 1069
SfaNI GCATC 2 cut(s) 1299, 1483
SfcI CTRYAG 1 cut(s) 227
Sfr303I CCGCGG 1 cut(s) 1289
SgrBI CCGCGG 1 cut(s) 1289
SinI GGWCC 4 cut(s) 187, 240, 247, 1445
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 4 cut(s) 14, 164, 992, 1304
SmoI CTYRAG 4 cut(s) 14, 164, 992, 1304
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 3 cut(s) 728, 1286, 1288
SspMI CTAG 2 cut(s) 1425, 1573
SstI GAGCTC 1 cut(s) 1069
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 1 cut(s) 421
StyI CCWWGG 2 cut(s) 89, 243
TaaI ACNGT 4 cut(s) 52, 139, 440, 1114
TaqI TCGA 4 cut(s) 141, 1092, 1200, 1239
TatI WGTACW 1 cut(s) 1464
TauI GCSGC 1 cut(s) 1288
TfiI GAWTC 6 cut(s) 376, 714, 898, 1128, 1156, 1202
Tru1I TTAA 3 cut(s) 557, 747, 755
Tru9I TTAA 3 cut(s) 557, 747, 755
TscAI CASTG 8 cut(s) 57, 87, 151, 598, 694, 832, 1236, 1336
TseFI GTSAC 3 cut(s) 78, 359, 685
TseI GCWGC 5 cut(s) 116, 226, 575, 1039, 1496
Tsp45I GTSAC 3 cut(s) 78, 359, 685
TspDTI ATGAA 9 cut(s) 31, 80, 279, 467, 495, 581, 861, 911, 1416
TspGWI ACGGA 1 cut(s) 1382
TspRI CASTG 8 cut(s) 57, 87, 151, 598, 694, 832, 1236, 1336
VpaK11BI GGWCC 4 cut(s) 187, 240, 247, 1445
XapI RAATTY 1 cut(s) 1048
XceI RCATGY 1 cut(s) 1433
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 2 cut(s) 612, 1388
XspI CTAG 2 cut(s) 1425, 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.