Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004725

Physical Location & Seq

Genomic Coordinates Forward (+)

28367241 .. 28372586

Gene Structure 5346 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1221000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGGTAATCCGTTCGTGAGAAGCGGGTTGAGCTTCTCCGCCATGTTTCCAACCCACATTCTTCCGCCGCTTAAACCCTTGCACGAAACAGTCCTCACTAGAATTCCGATTGGATTTCCGAAGCTCCGGCCGACGCTTCCGCCGCACTCCTCCTCCTCCTCCTCCCCCTCCTCCTCCTCCTCAAACTTTGATATCATTTCCACAAGCGAGTTGCGAGATGGTAGCTTTCTCTTCCGCTTTGGGGACGAGAGCGAGAAATCTGTTGCCACTGAAACAGAGATTGATGCTCGTAGTCATCTGGAGGATCCTGTGCAAGATTCAGAATTGAGCCAAACATTGTTGGGAAGCACAGGAAGTGGGGCTGGTGGCGGTGCAGGTGCCGAAGAGAATGTTATTGATGCGGTTTCAGTGACGAGCACCGACAATTCGACTCACGGTGGTGTAGGTGAAGAAGCCACAACCACAACCACAATCACAATCACAACCACAGCAGATGAAACCCAGGTCAGCAATATAACTCATGCTTATCCAGCAATTGGCGGCGCCACGGAGGCCGAGAATGGTGTTAATGTGGTTTCAGTGACGAGCACTGGCAGTGCGACTGCTGCTGGTGGTGGTGGTGAAGAAATCACAATTAGAACCACAACCACAACTACAGCAGATCAAACTCAAGTCAGCAATGAAATCCATGCTTTTGGAGCAAGTGGCTGTGACAGAGCCGCAAAGGCCGAGGAGAATGCTGTTGATGCGGTTTCAGTGACGAGCACCGAAGATGCAACTGGCGGCAGTGGTGGTGAACAAACCACAACCGCGAGCACAACCACAGCGGATGAGACTCAAGTCTCCTTCGTGTTAGCTGAGAATGCCCCTTCCACAAATTCAGAAGATGAATTCGCAGAGGGCGAAGCACATAGAAGTGACGTTTCAGGAGTATCAACTTCTAAGATAGTGGAATTCCATACAGTTGAGGGCGCTTCTGATGGGGAGGACGTACCTGCAGCTGTTCTTCAACTATCTTCCAGTGCTGCTTTATTACCACATCCCTCAAAGGCATTGACAGGTGGGGAGGATGCTTACTTTGTTGCTGGCCAAAACTGGTTAGGTGTGGCTGATGGAGTGGGTCAGTGGTCGCTAGAAGGGGTTAATCCTGGACTATATGCTCGTGAACTCATGGAGAATTGTGAAAGGTTCATATCCGATTGCAAAGGTTTACCATTGACTGAACCAGAAGAAGTCCTTATTAGAGGCTTATCCAGTACAAAATCTCCTGGATCATCCACAGTGTTAGTTGCTTATTTTGATGGTCAGGCTCTCCATGTGGCCAACATTGGAAACTCGGGATTTATTGTAATTAGAAATGGTGCTGTCTTTAAAAAATCATCACCAATGGTTCACGAGTTCAATTTTCCAGTACGGGTTGAAAGCGGTGATGATCCCTCAAAACTTTTAGAGAAATACCGAATTGATTTGGATGTGGGCGATGTGGTTGTCACTGCAACAGATGGTCTCTTCAACAACTTGTATGAGCAAGAGATCACCTCAGTTGTATCGAAGTCACTGCAAACCGGGTTGGAACTACAGGACATAGCAGAGGTCTTGGCCACTAGTGCACAAGAGTTGGGGCAATCTAAATCTACAAGGAGCCCATTTGCTGATGCAGCCAAGGCACATGGGTATGCCGGATATAGTGGCGGCAAGCTTGATGATGTGGTCGTTATCCTGTCATGTGTAGAAAAGAAACCCATCGGTACTTTACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C SpoIIE

Protein Analysis

587

Amino Acids

60.85

Weight (kDa)

4.47

Isoelectric Point (pI)

40.76

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004725)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33500
fragaria_vesca	FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930 FvH4_5g30930
malus_domestica	MD08G1221000.v1.1 MD15G1411700.v1.1
prunus_persica	Prupe.1G557900_v2.0.a1
pyrus_communis	pycom08g19170 pycom15g36650
rosa_chinensis	RchiOBHm_Chr7g0226241
rosa_laevigata	RLG00000001755
rosa_multiflora	Rmu_sc0002055.1_g000001 Rmu_sc0013267.1_g000003
rosa_roxburghii	Rroxscaffold_3G00233100
rosa_rugosa	Rorug07G0235800 Rorug07G0235900
rosa_samantha	Rh7AG382800 Rh7BG370000 Rh7CG402300 Rh7DG381200
rosa_wichuraiana	Rw0G018620 Rw0G021000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	365
Acc36I	ACCTGC	2 cut(s)	365, 1003
AccB1I	GGYRCC	2 cut(s)	377, 542
AccB7I	CCANNNNNTGG	1 cut(s)	536
AccII	CGCG	1 cut(s)	812
AclWI	GGATC	4 cut(s)	299, 312, 1279, 1427
AcoI	YGGCCR	4 cut(s)	128, 1087, 1320, 1599
AcsI	RAATTY	4 cut(s)	102, 877, 890, 953
AcyI	GRCGYC	1 cut(s)	543
AfaI	GTAC	4 cut(s)	993, 1258, 1413, 1750
AfiI	CCNNNNNNNGG	2 cut(s)	241, 536
AgsI	TTSAA	4 cut(s)	1010, 1402, 1421, 1513
AhlI	ACTAGT	1 cut(s)	1604
AjnI	CCWGG	3 cut(s)	501, 1147, 1267
AleI	CACNNNNGTG	1 cut(s)	438
AluBI	AGCT	6 cut(s)	33, 124, 225, 857, 1001, 1699
AluI	AGCT	6 cut(s)	33, 124, 225, 857, 1001, 1699
Alw21I	GWGCWC	5 cut(s)	419, 590, 767, 818, 1612
Alw26I	GTCTC	3 cut(s)	827, 847, 1511
Alw44I	GTGCAC	1 cut(s)	1608
AlwI	GGATC	4 cut(s)	299, 312, 1279, 1427
Ama87I	CYCGRG	1 cut(s)	1336
AoxI	GGCC	6 cut(s)	128, 552, 726, 1087, 1320, 1599
ApaLI	GTGCAC	1 cut(s)	1608
ApeKI	GCWGC	4 cut(s)	605, 998, 1025, 1658
ApoI	RAATTY	4 cut(s)	102, 877, 890, 953
AspLEI	GCGC	2 cut(s)	545, 974
AsuC2I	CCSGG	1 cut(s)	1567
AsuHPI	GGTGA	6 cut(s)	458, 632, 806, 1374, 1439, 1528
AvaI	CYCGRG	1 cut(s)	1336
BaeGI	GKGCMC	1 cut(s)	1612
BalI	TGGCCA	3 cut(s)	1089, 1322, 1601
BamHI	GGATCC	1 cut(s)	304
BanI	GGYRCC	2 cut(s)	377, 542
BanII	GRGCYC	1 cut(s)	1646
BauI	CACGAG	2 cut(s)	1161, 1394
Bbv12I	GWGCWC	5 cut(s)	419, 590, 767, 818, 1612
BbvI	GCAGC	4 cut(s)	592, 1010, 1012, 1670
BccI	CCATC	6 cut(s)	212, 974, 1106, 1295, 1496, 1751
BcgI	CGANNNNNNTGC	4 cut(s)	719, 753, 1540, 1574
BciT130I	CCWGG	3 cut(s)	503, 1149, 1269
BcnI	CCSGG	1 cut(s)	1567
BcoDI	GTCTC	3 cut(s)	827, 847, 1511
BcuI	ACTAGT	1 cut(s)	1604
BfaI	CTAG	3 cut(s)	99, 1133, 1605
BfmI	CTRYAG	3 cut(s)	654, 996, 1577
BfoI	RGCGCY	2 cut(s)	546, 975
BfuAI	ACCTGC	2 cut(s)	365, 1003
BglI	GCCNNNNNGGC	2 cut(s)	551, 725
Bme1390I	CCNGG	4 cut(s)	503, 1149, 1269, 1567
BmeT110I	CYCGRG	1 cut(s)	1336
BmiI	GGNNCC	4 cut(s)	306, 379, 544, 1643
BmrFI	CCNGG	4 cut(s)	503, 1149, 1269, 1567
BmsI	GCATC	6 cut(s)	274, 388, 736, 763, 1060, 1645
BoxI	GACNNNNGTC	1 cut(s)	839
BplI	GAGNNNNNCTC	2 cut(s)	1523, 1555
BpmI	CTGGAG	1 cut(s)	320
BpuEI	CTTGAG	2 cut(s)	654, 822
BpuMI	CCSGG	1 cut(s)	1567
BsaHI	GRCGYC	1 cut(s)	543
BsaI	GGTCTC	1 cut(s)	1511
BsaJI	CCNNGG	4 cut(s)	501, 546, 729, 1662
BsaXI	ACNNNNNCTCC	4 cut(s)	137, 167, 1249, 1279
Bsc4I	CCNNNNNNNGG	2 cut(s)	241, 536
Bse1I	ACTGG	6 cut(s)	595, 784, 1020, 1100, 1254, 1409
Bse3DI	GCAATG	1 cut(s)	685
BseBI	CCWGG	3 cut(s)	503, 1149, 1269
BseDI	CCNNGG	4 cut(s)	501, 546, 729, 1662
BseGI	GGATG	5 cut(s)	835, 1039, 1075, 1274, 1477
BseLI	CCNNNNNNNGG	2 cut(s)	241, 536
BseMI	GCAATG	1 cut(s)	685
BseMII	CTCAG	2 cut(s)	849, 1554
BseNI	ACTGG	6 cut(s)	595, 784, 1020, 1100, 1254, 1409
BseSI	GKGCMC	1 cut(s)	1612
BseX3I	CGGCCG	1 cut(s)	128
BseXI	GCAGC	4 cut(s)	592, 1010, 1012, 1670
BsgI	GTGCAG	1 cut(s)	393
Bsh1236I	CGCG	1 cut(s)	812
Bsh1285I	CGRYCG	1 cut(s)	131
BshFI	GGCC	6 cut(s)	130, 554, 728, 1089, 1322, 1601
BshNI	GGYRCC	2 cut(s)	377, 542
BsiEI	CGRYCG	1 cut(s)	131
BsiHKAI	GWGCWC	5 cut(s)	419, 590, 767, 818, 1612
BsiHKCI	CYCGRG	1 cut(s)	1336
BsiSI	CCGG	3 cut(s)	127, 1566, 1680
BslFI	GGGAC	1 cut(s)	257
BslI	CCNNNNNNNGG	2 cut(s)	241, 536
BsmAI	GTCTC	3 cut(s)	827, 847, 1511
BsmFI	GGGAC	1 cut(s)	257
BsmI	GAATGC	2 cut(s)	742, 868
BsnI	GGCC	6 cut(s)	130, 554, 728, 1089, 1322, 1601
Bso31I	GGTCTC	1 cut(s)	1511
BsoBI	CYCGRG	1 cut(s)	1336
Bsp1286I	GDGCHC	6 cut(s)	419, 590, 767, 818, 1612, 1646
Bsp143I	GATC	5 cut(s)	304, 661, 1271, 1432, 1533
BspANI	GGCC	6 cut(s)	130, 554, 728, 1089, 1322, 1601
BspCNI	CTCAG	2 cut(s)	850, 1553
BspFNI	CGCG	1 cut(s)	812
BspLI	GGNNCC	4 cut(s)	306, 379, 544, 1643
BspMAI	CTGCAG	1 cut(s)	1000
BspMI	ACCTGC	2 cut(s)	365, 1003
BspPI	GGATC	4 cut(s)	299, 312, 1279, 1427
BspT107I	GGYRCC	2 cut(s)	377, 542
BspTNI	GGTCTC	1 cut(s)	1511
BsrDI	GCAATG	1 cut(s)	685
BsrI	ACTGG	6 cut(s)	595, 784, 1020, 1100, 1254, 1409
BssECI	CCNNGG	4 cut(s)	501, 546, 729, 1662
BssMI	GATC	5 cut(s)	304, 661, 1271, 1432, 1533
BssNI	GRCGYC	1 cut(s)	543
BssSI	CACGAG	2 cut(s)	1161, 1394
BssT1I	CCWWGG	1 cut(s)	1662
Bst2BI	CACGAG	2 cut(s)	1161, 1394
Bst2UI	CCWGG	3 cut(s)	503, 1149, 1269
Bst4CI	ACNGT	5 cut(s)	91, 437, 964, 1282, 1758
Bst6I	CTCTTC	3 cut(s)	236, 378, 1514
BstACI	GRCGYC	1 cut(s)	543
BstC8I	GCNNGC	3 cut(s)	814, 1087, 1697
BstDEI	CTNAG	3 cut(s)	858, 942, 1540
BstDSI	CCRYGG	1 cut(s)	546
BstF5I	GGATG	5 cut(s)	835, 1039, 1075, 1274, 1477
BstFNI	CGCG	1 cut(s)	812
BstH2I	RGCGCY	2 cut(s)	546, 975
BstHHI	GCGC	2 cut(s)	545, 974
BstKTI	GATC	5 cut(s)	307, 664, 1274, 1435, 1536
BstMAI	GTCTC	3 cut(s)	827, 847, 1511
BstMBI	GATC	5 cut(s)	304, 661, 1271, 1432, 1533
BstMCI	CGRYCG	1 cut(s)	131
BstNI	CCWGG	3 cut(s)	503, 1149, 1269
BstPAI	GACNNNNGTC	1 cut(s)	839
BstSCI	CCNGG	4 cut(s)	501, 1147, 1267, 1565
BstSFI	CTRYAG	3 cut(s)	654, 996, 1577
BstSLI	GKGCMC	1 cut(s)	1612
BstUI	CGCG	1 cut(s)	812
BstV1I	GCAGC	4 cut(s)	592, 1010, 1012, 1670
BstX2I	RGATCY	1 cut(s)	304
BstXI	CCANNNNNNTGG	1 cut(s)	695
BstYI	RGATCY	1 cut(s)	304
BstZI	CGGCCG	1 cut(s)	128
BsuRI	GGCC	6 cut(s)	130, 554, 728, 1089, 1322, 1601
BtgI	CCRYGG	1 cut(s)	546
BtgZI	GCGATG	1 cut(s)	1494
BtsCI	GGATG	5 cut(s)	835, 1039, 1075, 1274, 1477
BtsI	GCAGTG	4 cut(s)	601, 793, 1491, 1556
BveI	ACCTGC	2 cut(s)	365, 1003
Cac8I	GCNNGC	3 cut(s)	814, 1087, 1697
CfoI	GCGC	2 cut(s)	545, 974
CseI	GACGC	1 cut(s)	142
Csp6I	GTAC	4 cut(s)	992, 1257, 1412, 1749
CviAII	CATG	7 cut(s)	43, 521, 689, 1171, 1316, 1670, 1725
CviQI	GTAC	4 cut(s)	992, 1257, 1412, 1749
DdeI	CTNAG	3 cut(s)	858, 942, 1540
DinI	GGCGCC	1 cut(s)	544
DpnI	GATC	5 cut(s)	306, 663, 1273, 1434, 1535
DpnII	GATC	5 cut(s)	304, 661, 1271, 1432, 1533
DraI	TTTAAA	1 cut(s)	1372
EaeI	YGGCCR	4 cut(s)	128, 1087, 1320, 1599
EagI	CGGCCG	1 cut(s)	128
Eam1104I	CTCTTC	3 cut(s)	236, 378, 1514
EarI	CTCTTC	3 cut(s)	236, 378, 1514
EciI	GGCGGA	3 cut(s)	28, 54, 129
EclXI	CGGCCG	1 cut(s)	128
Eco130I	CCWWGG	1 cut(s)	1662
Eco24I	GRGCYC	1 cut(s)	1646
Eco31I	GGTCTC	1 cut(s)	1511
Eco32I	GATATC	1 cut(s)	193
Eco52I	CGGCCG	1 cut(s)	128
Eco88I	CYCGRG	1 cut(s)	1336
EcoRI	GAATTC	3 cut(s)	102, 890, 953
EcoRII	CCWGG	3 cut(s)	501, 1147, 1267
EcoRV	GATATC	1 cut(s)	193
EcoT14I	CCWWGG	1 cut(s)	1662
EcoT38I	GRGCYC	1 cut(s)	1646
EgeI	GGCGCC	1 cut(s)	544
EheI	GGCGCC	1 cut(s)	544
ErhI	CCWWGG	1 cut(s)	1662
FaeI	CATG	7 cut(s)	46, 524, 692, 1174, 1319, 1673, 1728
FalI	AAGNNNNNCTT	2 cut(s)	1221, 1253
FaqI	GGGAC	1 cut(s)	257
FatI	CATG	7 cut(s)	42, 520, 688, 1170, 1315, 1669, 1724
FauI	CCCGC	1 cut(s)	17
FokI	GGATG	5 cut(s)	842, 1026, 1082, 1261, 1484
FriOI	GRGCYC	1 cut(s)	1646
FspBI	CTAG	3 cut(s)	99, 1133, 1605
GlaI	GCGC	2 cut(s)	544, 973
GsuI	CTGGAG	1 cut(s)	320
HaeII	RGCGCY	2 cut(s)	546, 975
HaeIII	GGCC	6 cut(s)	130, 554, 728, 1089, 1322, 1601
HapII	CCGG	3 cut(s)	127, 1566, 1680
HgaI	GACGC	1 cut(s)	142
HhaI	GCGC	2 cut(s)	545, 974
Hin1I	GRCGYC	1 cut(s)	543
Hin1II	CATG	7 cut(s)	46, 524, 692, 1174, 1319, 1673, 1728
Hin6I	GCGC	2 cut(s)	543, 972
HinP1I	GCGC	2 cut(s)	543, 972
HindIII	AAGCTT	1 cut(s)	1697
HinfI	GANTC	3 cut(s)	317, 430, 835
HpaII	CCGG	3 cut(s)	127, 1566, 1680
HphI	GGTGA	6 cut(s)	458, 632, 806, 1374, 1439, 1528
Hpy166II	GTNNAC	5 cut(s)	797, 1166, 1211, 1393, 1610
Hpy188I	TCNGA	6 cut(s)	108, 120, 322, 883, 979, 1198
Hpy188III	TCNNGA	6 cut(s)	16, 299, 927, 1163, 1338, 1394
Hpy8I	GTNNAC	5 cut(s)	797, 1166, 1211, 1393, 1610
Hpy99I	CGWCG	1 cut(s)	136
HpyAV	CCTTC	3 cut(s)	856, 879, 1130
HpyCH4III	ACNGT	5 cut(s)	91, 437, 964, 1282, 1758
HpyCH4IV	ACGT	2 cut(s)	921, 990
HpyF3I	CTNAG	3 cut(s)	858, 942, 1540
HpySE526I	ACGT	2 cut(s)	921, 990
Hsp92I	GRCGYC	1 cut(s)	543
Hsp92II	CATG	7 cut(s)	46, 524, 692, 1174, 1319, 1673, 1728
HspAI	GCGC	2 cut(s)	543, 972
KasI	GGCGCC	1 cut(s)	542
Kzo9I	GATC	5 cut(s)	304, 661, 1271, 1432, 1533
LmnI	GCTCC	3 cut(s)	129, 698, 1641
Lsp1109I	GCAGC	4 cut(s)	592, 1010, 1012, 1670
LweI	GCATC	6 cut(s)	274, 388, 736, 763, 1060, 1645
MaeI	CTAG	3 cut(s)	99, 1133, 1605
MaeII	ACGT	2 cut(s)	921, 990
MaeIII	GTNAC	7 cut(s)	409, 580, 710, 757, 917, 1489, 1554
MalI	GATC	5 cut(s)	306, 663, 1273, 1434, 1535
MboI	GATC	5 cut(s)	304, 661, 1271, 1432, 1533
MfeI	CAATTG	1 cut(s)	534
MflI	RGATCY	1 cut(s)	304
MhlI	GDGCHC	6 cut(s)	419, 590, 767, 818, 1612, 1646
MlsI	TGGCCA	3 cut(s)	1089, 1322, 1601
MluNI	TGGCCA	3 cut(s)	1089, 1322, 1601
Mly113I	GGCGCC	1 cut(s)	543
MlyI	GAGTC	2 cut(s)	424, 829
MmeI	TCCRAC	2 cut(s)	74, 1551
Mox20I	TGGCCA	3 cut(s)	1089, 1322, 1601
MscI	TGGCCA	3 cut(s)	1089, 1322, 1601
MseI	TTAA	4 cut(s)	72, 567, 1143, 1371
MslI	CAYNNNNRTG	3 cut(s)	438, 915, 1674
Msp20I	TGGCCA	3 cut(s)	1089, 1322, 1601
MspA1I	CMGCKG	2 cut(s)	827, 1001
MspI	CCGG	3 cut(s)	127, 1566, 1680
MspR9I	CCNGG	4 cut(s)	503, 1149, 1269, 1567
MunI	CAATTG	1 cut(s)	534
Mva1269I	GAATGC	2 cut(s)	742, 868
MvaI	CCWGG	3 cut(s)	503, 1149, 1269
MvnI	CGCG	1 cut(s)	812
NarI	GGCGCC	1 cut(s)	543
NciI	CCSGG	1 cut(s)	1567
NdeII	GATC	5 cut(s)	304, 661, 1271, 1432, 1533
NlaIII	CATG	7 cut(s)	46, 524, 692, 1174, 1319, 1673, 1728
NlaIV	GGNNCC	4 cut(s)	306, 379, 544, 1643
NmeAIII	GCCGAG	2 cut(s)	580, 754
NmuCI	GTSAC	7 cut(s)	409, 580, 710, 757, 917, 1489, 1554
OliI	CACNNNNGTG	1 cut(s)	438
PaqCI	CACCTGC	1 cut(s)	365
PcsI	WCGNNNNNNNCGW	1 cut(s)	900
PctI	GAATGC	2 cut(s)	742, 868
PfeI	GAWTC	1 cut(s)	317
PflMI	CCANNNNNTGG	1 cut(s)	536
PfoI	TCCNGGA	2 cut(s)	1147, 1267
PleI	GAGTC	2 cut(s)	424, 829
PluTI	GGCGCC	1 cut(s)	546
PpsI	GAGTC	2 cut(s)	424, 829
PshAI	GACNNNNGTC	1 cut(s)	839
Psp6I	CCWGG	3 cut(s)	501, 1147, 1267
PspGI	CCWGG	3 cut(s)	501, 1147, 1267
PspN4I	GGNNCC	4 cut(s)	306, 379, 544, 1643
PstI	CTGCAG	1 cut(s)	1000
PsuI	RGATCY	1 cut(s)	304
PvuII	CAGCTG	1 cut(s)	1001
RsaI	GTAC	4 cut(s)	993, 1258, 1413, 1750
RsaNI	GTAC	4 cut(s)	992, 1257, 1412, 1749
RseI	CAYNNNNRTG	3 cut(s)	438, 915, 1674
SaqAI	TTAA	4 cut(s)	72, 567, 1143, 1371
Sau3AI	GATC	5 cut(s)	304, 661, 1271, 1432, 1533
SchI	GAGTC	2 cut(s)	424, 829
ScrFI	CCNGG	4 cut(s)	503, 1149, 1269, 1567
SduI	GDGCHC	6 cut(s)	419, 590, 767, 818, 1612, 1646
SfaNI	GCATC	6 cut(s)	274, 388, 736, 763, 1060, 1645
SfcI	CTRYAG	3 cut(s)	654, 996, 1577
SfoI	GGCGCC	1 cut(s)	544
SmiMI	CAYNNNNRTG	3 cut(s)	438, 915, 1674
SmlI	CTYRAG	2 cut(s)	669, 837
SmoI	CTYRAG	2 cut(s)	669, 837
SpeI	ACTAGT	1 cut(s)	1604
SspDI	GGCGCC	1 cut(s)	542
SspMI	CTAG	3 cut(s)	99, 1133, 1605
StyD4I	CCNGG	4 cut(s)	501, 1147, 1267, 1565
StyI	CCWWGG	1 cut(s)	1662
TaaI	ACNGT	5 cut(s)	91, 437, 964, 1282, 1758
TaiI	ACGT	2 cut(s)	924, 993
TaqI	TCGA	2 cut(s)	428, 1550
TatI	WGTACW	1 cut(s)	1256
TauI	GCSGC	6 cut(s)	70, 145, 543, 722, 786, 1695
TfiI	GAWTC	1 cut(s)	317
Tru1I	TTAA	4 cut(s)	72, 567, 1143, 1371
Tru9I	TTAA	4 cut(s)	72, 567, 1143, 1371
TseFI	GTSAC	7 cut(s)	409, 580, 710, 757, 917, 1489, 1554
TseI	GCWGC	4 cut(s)	605, 998, 1025, 1658
Tsp45I	GTSAC	7 cut(s)	409, 580, 710, 757, 917, 1489, 1554
TspDTI	ATGAA	4 cut(s)	510, 696, 903, 1180
TspGWI	ACGGA	1 cut(s)	563
Van91I	CCANNNNNTGG	1 cut(s)	536
VneI	GTGCAC	1 cut(s)	1608
XapI	RAATTY	4 cut(s)	102, 877, 890, 953
XspI	CTAG	3 cut(s)	99, 1133, 1605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.