AT4G34970

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011901
Physical Location & Seq
Forward (+)
16653704 .. 16654955
1252 bp
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UTR
Exon/CDS
Intron
AT4G34970.1

Sequence Viewer

Length: 426 bp
ATGGCTTTGAAGACGGCGACGAGTGGTATGTGGATGACGGATGATTGCAAGAAATCGTTCATGGAGATGAAATGGAAGAAAGTGCATAGATACGTCGTTTACAAACTCGAGGAGAAGTCTCGGAAAGTCACCGTCGACAAGGTTGGTGCCGCCGGCGAGAGCTACGACGATCTCGCTGCTTCTTTGCCGGAGGATGACTGTCGTTACGCCGTGTTTGATTTCGATTACGTCACCGTCGATAACTGTCGTATGAGCAAGATCTTCTTCATAACTTGGTCGCCGGAGGCTTCAAGGATAAGGGAGAAGATGATGTACGCGACGTCGAAGAGCGGACTGAGAAGAGTGTTGGATGGTGTTCACTACGAGCTTCAAGCCACCGACCCAACCGAGATGGGATTTGATAAAATCCAGGACCGGGCCAAATGA

Protein Analysis

141

Amino Acids

16.37

Weight (kDa)

7.66

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 11 - 140 2.4e-40 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16700 AT2G16700 AT2G16700 AT4G34970
fragaria_vesca FvH4_2g01430
malus_domestica MD05G1119700.v1.1 MD10G1123400.v1.1
prunus_persica Prupe.8G163900_v2.0.a1
pyrus_communis pycom05g11370 pycom10g10570
rosa_chinensis RchiOBHm_Chr6g0244061 RchiOBHm_Chr6g0252411
rosa_laevigata RLG00000015386
rosa_multiflora Rmu_sc0006711.1_g000002
rosa_roxburghii Rroxscaffold_7G00216110
rosa_rugosa Rorug05G0507100
rosa_samantha Rh6AG021500 Rh6BG017200
rosa_wichuraiana Rw6G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 146
AccBSI CCGCTC 1 cut(s) 330
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 317
AciI CCGC 2 cut(s) 150, 330
AcyI GRCGYC 1 cut(s) 320
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 3 cut(s) 10, 291, 371
AjnI CCWGG 1 cut(s) 408
AluBI AGCT 2 cut(s) 162, 367
AluI AGCT 2 cut(s) 162, 367
Alw26I GTCTC 1 cut(s) 123
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 1 cut(s) 417
ApeKI GCWGC 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 56
AspS9I GGNCC 2 cut(s) 412, 417
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 2 cut(s) 121, 223
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 1 cut(s) 412
BanI GGYRCC 1 cut(s) 146
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbsI GAAGAC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 163
BccI CCATC 2 cut(s) 344, 385
BceAI ACGGC 2 cut(s) 30, 194
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 410
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 123
BglII AGATCT 1 cut(s) 258
BisI GCNGC 2 cut(s) 150, 177
BlsI GCNGC 2 cut(s) 151, 178
Bme1390I CCNGG 2 cut(s) 410, 416
Bme18I GGWCC 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 2 cut(s) 412, 417
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 2 cut(s) 410, 416
BpiI GAAGAC 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 320
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse118I RCCGGY 1 cut(s) 152
BseBI CCWGG 1 cut(s) 410
BseGI GGATG 4 cut(s) 39, 46, 199, 355
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 326
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 1 cut(s) 419
BshNI GGYRCC 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 4 cut(s) 153, 188, 281, 415
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 1 cut(s) 419
BsoBI CYCGRG 1 cut(s) 107
Bsp143I GATC 2 cut(s) 169, 258
BspACI CCGC 2 cut(s) 150, 330
BspANI GGCC 1 cut(s) 419
BspCNI CTCAG 1 cut(s) 327
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 152
BssAI RCCGGY 1 cut(s) 152
BssMI GATC 2 cut(s) 169, 258
BssNI GRCGYC 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 4 cut(s) 133, 200, 235, 245
Bst6I CTCTTC 2 cut(s) 320, 334
BstACI GRCGYC 1 cut(s) 320
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 1 cut(s) 335
BstF5I GGATG 4 cut(s) 39, 46, 199, 355
BstFNI CGCG 1 cut(s) 317
BstKTI GATC 2 cut(s) 172, 261
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 2 cut(s) 169, 258
BstNI CCWGG 1 cut(s) 410
BstSCI CCNGG 2 cut(s) 408, 414
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 1 cut(s) 163
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BsuRI GGCC 1 cut(s) 419
BtsCI GGATG 4 cut(s) 39, 46, 199, 355
Cac8I GCNNGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 412, 417
Csp6I GTAC 1 cut(s) 313
CviAII CATG 1 cut(s) 61
CviJI RGCY 6 cut(s) 5, 162, 287, 367, 374, 419
CviKI_1 RGCY 6 cut(s) 5, 162, 287, 367, 374, 419
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 335
DpnI GATC 2 cut(s) 171, 260
DpnII GATC 2 cut(s) 169, 258
Eam1104I CTCTTC 2 cut(s) 320, 334
EarI CTCTTC 2 cut(s) 320, 334
Eco47I GGWCC 1 cut(s) 412
Eco88I CYCGRG 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 408
FaeI CATG 1 cut(s) 64
FaiI YATR 5 cut(s) 29, 62, 87, 251, 269
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FatI CATG 1 cut(s) 60
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 150, 177
FokI GGATG 4 cut(s) 46, 53, 206, 362
Fsp4HI GCNGC 2 cut(s) 150, 177
GluI GCNGC 2 cut(s) 150, 177
HaeIII GGCC 1 cut(s) 419
HapII CCGG 4 cut(s) 153, 188, 281, 415
Hin1I GRCGYC 1 cut(s) 320
Hin1II CATG 1 cut(s) 64
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HpaII CCGG 4 cut(s) 153, 188, 281, 415
HphI GGTGA 2 cut(s) 121, 223
Hpy166II GTNNAC 3 cut(s) 100, 136, 358
Hpy188I TCNGA 1 cut(s) 123
Hpy8I GTNNAC 3 cut(s) 100, 136, 358
Hpy99I CGWCG 7 cut(s) 22, 98, 137, 170, 239, 322, 325
HpyCH4III ACNGT 4 cut(s) 133, 200, 235, 245
HpyCH4IV ACGT 3 cut(s) 93, 228, 320
HpyCH4V TGCA 2 cut(s) 48, 85
HpyF3I CTNAG 1 cut(s) 335
HpySE526I ACGT 3 cut(s) 93, 228, 320
Hsp92I GRCGYC 1 cut(s) 320
Hsp92II CATG 1 cut(s) 64
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 169, 258
LguI GCTCTTC 1 cut(s) 320
LpnPI CCDG 5 cut(s) 166, 201, 294, 395, 422
Lsp1109I GCAGC 1 cut(s) 163
MaeII ACGT 3 cut(s) 93, 228, 320
MaeIII GTNAC 3 cut(s) 127, 203, 229
MalI GATC 2 cut(s) 171, 260
MbiI CCGCTC 1 cut(s) 330
MboI GATC 2 cut(s) 169, 258
MboII GAAGA 7 cut(s) 22, 88, 253, 256, 316, 337, 351
MflI RGATCY 1 cut(s) 258
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 3 cut(s) 103, 184, 277
MreI CGCCGGCG 1 cut(s) 152
MroNI GCCGGC 1 cut(s) 152
MroXI GAANNNNTTC 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 65
MspI CCGG 4 cut(s) 153, 188, 281, 415
MspR9I CCNGG 2 cut(s) 410, 416
MvaI CCWGG 1 cut(s) 410
MvnI CGCG 1 cut(s) 317
NaeI GCCGGC 1 cut(s) 154
NciI CCSGG 1 cut(s) 416
NdeII GATC 2 cut(s) 169, 258
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 1 cut(s) 64
NlaIV GGNNCC 1 cut(s) 148
NmuCI GTSAC 2 cut(s) 127, 229
PaeR7I CTCGAG 1 cut(s) 107
PciSI GCTCTTC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdiI GCCGGC 1 cut(s) 154
PdmI GAANNNNTTC 1 cut(s) 56
PfoI TCCNGGA 1 cut(s) 408
PkrI GCNGC 2 cut(s) 151, 178
Psp6I CCWGG 1 cut(s) 408
PspGI CCWGG 1 cut(s) 408
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 2 cut(s) 412, 417
PspXI VCTCGAGB 1 cut(s) 107
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 65
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 320
SatI GCNGC 2 cut(s) 150, 177
Sau3AI GATC 2 cut(s) 169, 258
Sau96I GGNCC 2 cut(s) 412, 417
ScrFI CCNGG 2 cut(s) 410, 416
SetI ASST 6 cut(s) 96, 144, 164, 231, 323, 369
Sfr274I CTCGAG 1 cut(s) 107
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 1 cut(s) 412
SlaI CTCGAG 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SsiI CCGC 2 cut(s) 150, 330
StyD4I CCNGG 2 cut(s) 408, 414
TaaI ACNGT 4 cut(s) 133, 200, 235, 245
TaiI ACGT 3 cut(s) 96, 231, 323
TaqI TCGA 5 cut(s) 108, 135, 222, 237, 323
TauI GCSGC 1 cut(s) 152
TseFI GTSAC 2 cut(s) 127, 229
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 2 cut(s) 127, 229
TspDTI ATGAA 3 cut(s) 49, 83, 256
TspGWI ACGGA 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 56
ZraI GACGTC 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.