Rroxscaffold_7G00216110

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011901
Physical Location & Seq
Forward (+)
66807698 .. 66809043
1346 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00216110.1

Sequence Viewer

Length: 432 bp
ATGGCAATGGCTTTCAAGATGGCCACAACTGGGATGTGGGTTACTGATGAATGCAAGAACTCATTCCTGGAGATGAAATGGAAGAAGGTGGCTAGGTACATAGTGTACAAGATTGATGAAGAATCGAGGCTTGTGACCGTTGATAAGGTGGGTGGACCTGGTGAAAGCTATGATGATCTTGCAGCTTCTCTGCCCAAGGATGATTGCAGATATGCTGTTTTCGATTTCGATTTCGTCACCGTGGATAATTGCAAGAAGAGCAAAATCTTCTTCATTGCTTGGTCTCCAAGTGAATCAAGGATTAGAGCAAAAATCCTGTATGCAACCTCCAAGGATGGGTTGAGGAGAGTTCTTGATGGCATCAGCTATGAACTTCAGGCTACTGACCCTACTGAGATGGGGATGGATGTGATTAAGGACAGGGCTAAATAA

Protein Analysis

143

Amino Acids

16.32

Weight (kDa)

6.33

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 12 - 142 2.8e-37 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16700 AT2G16700 AT2G16700 AT4G34970
fragaria_vesca FvH4_2g01430
malus_domestica MD05G1119700.v1.1 MD10G1123400.v1.1
prunus_persica Prupe.8G163900_v2.0.a1
pyrus_communis pycom05g11370 pycom10g10570
rosa_chinensis RchiOBHm_Chr6g0244061 RchiOBHm_Chr6g0252411
rosa_laevigata RLG00000015386
rosa_multiflora Rmu_sc0006711.1_g000002
rosa_roxburghii Rroxscaffold_7G00216110
rosa_rugosa Rorug05G0507100
rosa_samantha Rh6AG021500 Rh6BG017200
rosa_wichuraiana Rw6G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 359
AfaI GTAC 2 cut(s) 98, 107
AfiI CCNNNNNNNGG 2 cut(s) 30, 336
AgsI TTSAA 1 cut(s) 16
AjnI CCWGG 2 cut(s) 66, 157
AluBI AGCT 3 cut(s) 168, 185, 366
AluI AGCT 3 cut(s) 168, 185, 366
Alw26I GTCTC 1 cut(s) 288
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 173, 229
AvaII GGWCC 1 cut(s) 155
BalI TGGCCA 1 cut(s) 23
BbvI GCAGC 1 cut(s) 194
BccI CCATC 5 cut(s) 13, 329, 350, 391, 397
BciT130I CCWGG 2 cut(s) 68, 159
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 93
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
Bme1390I CCNGG 2 cut(s) 68, 159
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 155
BmrFI CCNGG 2 cut(s) 68, 159
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 1 cut(s) 369
BmuI ACTGGG 1 cut(s) 39
BpmI CTGGAG 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 288
BsaJI CCNNGG 3 cut(s) 195, 240, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 336
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 2 cut(s) 12, 273
BseBI CCWGG 2 cut(s) 68, 159
BseDI CCNNGG 3 cut(s) 195, 240, 330
BseGI GGATG 5 cut(s) 39, 205, 340, 408, 412
BseLI CCNNNNNNNGG 2 cut(s) 30, 336
BseMI GCAATG 2 cut(s) 12, 273
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 358
BseXI GCAGC 1 cut(s) 194
BshFI GGCC 1 cut(s) 23
BslI CCNNNNNNNGG 2 cut(s) 30, 336
BsmAI GTCTC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 56
BsnI GGCC 1 cut(s) 23
Bso31I GGTCTC 1 cut(s) 288
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 1 cut(s) 175
BspANI GGCC 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 385
BspQI GCTCTTC 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 12, 273
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 195, 240, 330
BssMI GATC 1 cut(s) 175
BssT1I CCWWGG 2 cut(s) 195, 330
Bst2UI CCWGG 2 cut(s) 68, 159
Bst4CI ACNGT 2 cut(s) 139, 241
Bst6I CTCTTC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 105
BstDEI CTNAG 1 cut(s) 393
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 5 cut(s) 39, 205, 340, 408, 412
BstKTI GATC 1 cut(s) 178
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 258
BstNI CCWGG 2 cut(s) 68, 159
BstSCI CCNGG 2 cut(s) 66, 157
BstV1I GCAGC 1 cut(s) 194
BsuRI GGCC 1 cut(s) 23
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 5 cut(s) 39, 205, 340, 408, 412
Cfr13I GGNCC 1 cut(s) 155
CsiI ACCWGGT 1 cut(s) 157
Csp6I GTAC 2 cut(s) 97, 106
CviJI RGCY 9 cut(s) 11, 23, 92, 130, 168, 185, 366, 380, 425
CviKI_1 RGCY 9 cut(s) 11, 23, 92, 130, 168, 185, 366, 380, 425
CviQI GTAC 2 cut(s) 97, 106
DdeI CTNAG 1 cut(s) 393
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco130I CCWWGG 2 cut(s) 195, 330
Eco31I GGTCTC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 66, 157
EcoT14I CCWWGG 2 cut(s) 195, 330
ErhI CCWWGG 2 cut(s) 195, 330
FaiI YATR 5 cut(s) 101, 171, 213, 321, 369
Fnu4HI GCNGC 1 cut(s) 183
FokI GGATG 5 cut(s) 46, 212, 347, 415, 419
Fsp4HI GCNGC 1 cut(s) 183
FspBI CTAG 1 cut(s) 93
GluI GCNGC 1 cut(s) 183
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 1 cut(s) 23
HinfI GANTC 2 cut(s) 122, 293
HphI GGTGA 2 cut(s) 173, 229
Hpy166II GTNNAC 2 cut(s) 106, 155
Hpy188III TCNNGA 2 cut(s) 16, 353
Hpy8I GTNNAC 2 cut(s) 106, 155
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 2 cut(s) 139, 241
HpyCH4V TGCA 5 cut(s) 54, 182, 207, 252, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 258
HpyF3I CTNAG 1 cut(s) 393
Kzo9I GATC 1 cut(s) 175
LguI GCTCTTC 1 cut(s) 251
LpnPI CCDG 8 cut(s) 15, 53, 80, 144, 171, 329, 362, 406
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 1 cut(s) 369
MabI ACCWGGT 1 cut(s) 157
MaeI CTAG 1 cut(s) 93
MaeIII GTNAC 3 cut(s) 40, 133, 235
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 5 cut(s) 94, 131, 259, 262, 268
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 1 cut(s) 247
MluNI TGGCCA 1 cut(s) 23
MnlI CCTC 3 cut(s) 120, 336, 337
Mox20I TGGCCA 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 62
MscI TGGCCA 1 cut(s) 23
MseI TTAA 1 cut(s) 414
Msp20I TGGCCA 1 cut(s) 23
MspR9I CCNGG 2 cut(s) 68, 159
Mva1269I GAATGC 1 cut(s) 56
MvaI CCWGG 2 cut(s) 68, 159
MwoI GCNNNNNNNGC 1 cut(s) 258
NdeII GATC 1 cut(s) 175
NmuCI GTSAC 2 cut(s) 133, 235
PciSI GCTCTTC 1 cut(s) 251
PctI GAATGC 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 2 cut(s) 122, 293
PfoI TCCNGGA 1 cut(s) 66
PkrI GCNGC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 66, 157
PspGI CCWGG 2 cut(s) 66, 157
PspPI GGNCC 1 cut(s) 155
RsaI GTAC 2 cut(s) 98, 107
RsaNI GTAC 2 cut(s) 97, 106
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 1 cut(s) 175
Sau96I GGNCC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 68, 159
SetI ASST 8 cut(s) 90, 98, 150, 160, 170, 187, 329, 368
SexAI ACCWGGT 1 cut(s) 157
SfaNI GCATC 1 cut(s) 369
SinI GGWCC 1 cut(s) 155
Sse9I AATT 1 cut(s) 247
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 2 cut(s) 66, 157
StyI CCWWGG 2 cut(s) 195, 330
TaaI ACNGT 2 cut(s) 139, 241
TaqI TCGA 3 cut(s) 125, 222, 228
TasI AATT 1 cut(s) 247
TatI WGTACW 1 cut(s) 105
TfiI GAWTC 2 cut(s) 122, 293
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TseFI GTSAC 2 cut(s) 133, 235
TseI GCWGC 1 cut(s) 182
Tsp45I GTSAC 2 cut(s) 133, 235
TspDTI ATGAA 5 cut(s) 63, 89, 132, 262, 384
VpaK11BI GGWCC 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 62
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.