RchiOBHm_Chr6g0252411

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011901
Physical Location & Seq
Reverse (-)
7549519 .. 7550478
960 bp
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UTR
Exon/CDS
Intron
PRQ22632

Sequence Viewer

Length: 426 bp
ATGTTCTTTGTTATAGGCCACAACTGGGATGTGGTTACTGATGAATGCAAGAACTCATTCCTGGAGATGAAATGGAAGAAGGTGGCTAGGTACATAGTGTACAAGATTGATGAAGAATCGAGGCTTGTGACCGTTGATAAGGTGGGTGGACCTGGTGAAAGCTATGATGATCTTGCAGCTTCTCTGCCCAAAGATGATTGCAGATATGCTGTTTTCGATTTCGATTTCGTCACTGTGGATAATTGCAAGAAGAGCAAAATCTTCTTCATTGCTTGGTCTCCAAGTGAATCAAGGATTAGAGCAAAAATCCTGTATGCAACCTCCAAGGATGGGTTGAGGAGAGTTCTTGATGGCATCAGCTATGAACTTCAGGCTACTGACCCTACTGAGATGGGGATGGATGTGATTAAGGACAGGGCTAAATAA

Protein Analysis

141

Amino Acids

16.21

Weight (kDa)

5.48

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 12 - 140 6.2e-37 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16700 AT2G16700 AT2G16700 AT4G34970
fragaria_vesca FvH4_2g01430
malus_domestica MD05G1119700.v1.1 MD10G1123400.v1.1
prunus_persica Prupe.8G163900_v2.0.a1
pyrus_communis pycom05g11370 pycom10g10570
rosa_chinensis RchiOBHm_Chr6g0244061 RchiOBHm_Chr6g0252411
rosa_laevigata RLG00000015386
rosa_multiflora Rmu_sc0006711.1_g000002
rosa_roxburghii Rroxscaffold_7G00216110
rosa_rugosa Rorug05G0507100
rosa_samantha Rh6AG021500 Rh6BG017200
rosa_wichuraiana Rw6G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 353
AfaI GTAC 2 cut(s) 92, 101
AfiI CCNNNNNNNGG 2 cut(s) 25, 330
AjnI CCWGG 2 cut(s) 60, 151
AluBI AGCT 3 cut(s) 162, 179, 360
AluI AGCT 3 cut(s) 162, 179, 360
Alw26I GTCTC 1 cut(s) 282
AoxI GGCC 1 cut(s) 16
ApeKI GCWGC 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 167
AvaII GGWCC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 188
BccI CCATC 4 cut(s) 323, 344, 385, 391
BciT130I CCWGG 2 cut(s) 62, 153
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 1 cut(s) 87
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 62, 153
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 62, 153
BmrI ACTGGG 1 cut(s) 34
BmsI GCATC 1 cut(s) 363
BmuI ACTGGG 1 cut(s) 34
BpmI CTGGAG 1 cut(s) 83
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 324
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 330
Bse1I ACTGG 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 267
BseBI CCWGG 2 cut(s) 62, 153
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 4 cut(s) 34, 334, 402, 406
BseLI CCNNNNNNNGG 2 cut(s) 25, 330
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 1 cut(s) 29
BseRI GAGGAG 1 cut(s) 352
BseXI GCAGC 1 cut(s) 188
BshFI GGCC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 25, 330
BsmAI GTCTC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 1 cut(s) 18
Bso31I GGTCTC 1 cut(s) 282
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 1 cut(s) 169
BspANI GGCC 1 cut(s) 18
BspCNI CTCAG 1 cut(s) 379
BspQI GCTCTTC 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 282
BsrDI GCAATG 1 cut(s) 267
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 62, 153
Bst4CI ACNGT 2 cut(s) 133, 235
Bst6I CTCTTC 1 cut(s) 245
BstAUI TGTACA 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 387
BstF5I GGATG 4 cut(s) 34, 334, 402, 406
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNI CCWGG 2 cut(s) 62, 153
BstSCI CCNGG 2 cut(s) 60, 151
BstV1I GCAGC 1 cut(s) 188
BsuRI GGCC 1 cut(s) 18
BtsCI GGATG 4 cut(s) 34, 334, 402, 406
BtsIMutI CAGTG 1 cut(s) 231
Cfr13I GGNCC 1 cut(s) 149
CsiI ACCWGGT 1 cut(s) 151
Csp6I GTAC 2 cut(s) 91, 100
CviJI RGCY 8 cut(s) 18, 86, 124, 162, 179, 360, 374, 419
CviKI_1 RGCY 8 cut(s) 18, 86, 124, 162, 179, 360, 374, 419
CviQI GTAC 2 cut(s) 91, 100
DdeI CTNAG 1 cut(s) 387
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 353
EcoRII CCWGG 2 cut(s) 60, 151
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaiI YATR 6 cut(s) 14, 95, 165, 207, 315, 363
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 4 cut(s) 41, 341, 409, 413
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 1 cut(s) 87
GluI GCNGC 1 cut(s) 177
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 1 cut(s) 18
HinfI GANTC 2 cut(s) 116, 287
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 2 cut(s) 100, 149
Hpy188III TCNNGA 1 cut(s) 347
Hpy8I GTNNAC 2 cut(s) 100, 149
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 2 cut(s) 133, 235
HpyCH4V TGCA 5 cut(s) 48, 176, 201, 246, 317
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 1 cut(s) 387
Kzo9I GATC 1 cut(s) 169
LguI GCTCTTC 1 cut(s) 245
LpnPI CCDG 8 cut(s) 10, 47, 74, 138, 165, 323, 356, 400
Lsp1109I GCAGC 1 cut(s) 188
LweI GCATC 1 cut(s) 363
MabI ACCWGGT 1 cut(s) 151
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 3 cut(s) 34, 127, 229
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 5 cut(s) 88, 125, 253, 256, 262
MluCI AATT 1 cut(s) 241
MnlI CCTC 3 cut(s) 114, 330, 331
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 1 cut(s) 408
MspR9I CCNGG 2 cut(s) 62, 153
Mva1269I GAATGC 1 cut(s) 50
MvaI CCWGG 2 cut(s) 62, 153
MwoI GCNNNNNNNGC 1 cut(s) 252
NdeII GATC 1 cut(s) 169
NmuCI GTSAC 2 cut(s) 127, 229
PciSI GCTCTTC 1 cut(s) 245
PctI GAATGC 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 2 cut(s) 116, 287
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 1 cut(s) 178
Psp6I CCWGG 2 cut(s) 60, 151
PspGI CCWGG 2 cut(s) 60, 151
PspPI GGNCC 1 cut(s) 149
RsaI GTAC 2 cut(s) 92, 101
RsaNI GTAC 2 cut(s) 91, 100
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 62, 153
SetI ASST 8 cut(s) 84, 92, 144, 154, 164, 181, 323, 362
SexAI ACCWGGT 1 cut(s) 151
SfaNI GCATC 1 cut(s) 363
SinI GGWCC 1 cut(s) 149
Sse9I AATT 1 cut(s) 241
SspMI CTAG 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 60, 151
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 133, 235
TaqI TCGA 3 cut(s) 119, 216, 222
TasI AATT 1 cut(s) 241
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 2 cut(s) 116, 287
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 127, 229
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 2 cut(s) 127, 229
TspDTI ATGAA 5 cut(s) 57, 83, 126, 256, 378
TspRI CASTG 1 cut(s) 238
VpaK11BI GGWCC 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.