MD05G1119700.v1.1

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011901
Physical Location & Seq
Forward (+)
24134849 .. 24136412
1564 bp
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UTR
Exon/CDS
Intron
MD05G1119700.v1.1.491

Sequence Viewer

Length: 534 bp
ATGGCAAGGCAACACTCTGAGGACTCAGCCTTCAGAATCTCAGCACAGCAGAGAGCACAAGCAAAAGGAAAAGCAAAAATCCCCTTTCAAACTTCGGCAACCATGGCAATGGCTTTCAAGATGGCTACAACTGGGATGTGGGTGACTGATGAATGCAAGAACTCGTTCATGGAGATGAAATGGAAGAAGGTTCACAGATACATTGTTTTCAAGATCGATGAGGAATCGAGGCTGGTGACCGTTGATAAAGTTGGTGGGCCAGGGGAAAACTATGATGATCTGGCAGCCTCCTTGCCCAAAGATGATTGCAGATATGCAGTCTTTGATTTTGATTTTGTCACTGTTGATAACTGCAGGAAGAGCAAACTCTTCTTCATTGCTTGGTCACCAAGTGAATCAAGGATTAGAGCCAAAATTCTATATGCAACCTCCAAAGCTGGGCTGAGGAGAGTTTTAGATGGCATCAGCTATGAACTTCAGGCGACCGATCCGACCGAGATGGGGATGGATGTGATCAAGGACAGGGCTAAATAA

Protein Analysis

178

Amino Acids

20.13

Weight (kDa)

9.08

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 46 - 176 1.3e-37 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16700 AT2G16700 AT2G16700 AT4G34970
fragaria_vesca FvH4_2g01430
malus_domestica MD05G1119700.v1.1 MD10G1123400.v1.1
prunus_persica Prupe.8G163900_v2.0.a1
pyrus_communis pycom05g11370 pycom10g10570
rosa_chinensis RchiOBHm_Chr6g0244061 RchiOBHm_Chr6g0252411
rosa_laevigata RLG00000015386
rosa_multiflora Rmu_sc0006711.1_g000002
rosa_roxburghii Rroxscaffold_7G00216110
rosa_rugosa Rorug05G0507100
rosa_samantha Rh6AG021500 Rh6BG017200
rosa_wichuraiana Rw6G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 482
AcsI RAATTY 1 cut(s) 414
AcuI CTGAAG 2 cut(s) 16, 461
AdeI CACNNNGTG 1 cut(s) 392
AfiI CCNNNNNNNGG 2 cut(s) 438, 501
AgsI TTSAA 3 cut(s) 89, 118, 211
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 2 cut(s) 437, 468
AluI AGCT 2 cut(s) 437, 468
Alw21I GWGCWC 1 cut(s) 58
AlwI GGATC 1 cut(s) 482
AoxI GGCC 1 cut(s) 257
ApeKI GCWGC 1 cut(s) 284
ApoI RAATTY 1 cut(s) 414
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 3 cut(s) 154, 247, 378
Bbv12I GWGCWC 1 cut(s) 58
BbvCI CCTCAGC 1 cut(s) 443
BbvI GCAGC 1 cut(s) 296
BccI CCATC 4 cut(s) 115, 452, 493, 499
BciT130I CCWGG 1 cut(s) 261
BclI TGATCA 1 cut(s) 513
BfmI CTRYAG 1 cut(s) 352
BisI GCNGC 1 cut(s) 285
BlsI GCNGC 1 cut(s) 286
Bme1390I CCNGG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 261
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 471
BmuI ACTGGG 1 cut(s) 141
Bpu10I CCTNAGC 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 102, 260
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 501
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 2 cut(s) 114, 375
BseBI CCWGG 1 cut(s) 261
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 2 cut(s) 102, 260
BseGI GGATG 3 cut(s) 141, 510, 514
BseLI CCNNNNNNNGG 2 cut(s) 438, 501
BseMI GCAATG 2 cut(s) 114, 375
BseMII CTCAG 4 cut(s) 9, 39, 54, 434
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 460
BseXI GCAGC 1 cut(s) 296
BseYI CCCAGC 1 cut(s) 437
Bsh1285I CGRYCG 2 cut(s) 486, 495
BshFI GGCC 1 cut(s) 259
BshVI ATCGAT 1 cut(s) 216
BsiEI CGRYCG 2 cut(s) 486, 495
BsiHKAI GWGCWC 1 cut(s) 58
BslI CCNNNNNNNGG 2 cut(s) 438, 501
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 4 cut(s) 213, 277, 487, 513
Bsp19I CCATGG 1 cut(s) 102
BspANI GGCC 1 cut(s) 259
BspCNI CTCAG 4 cut(s) 10, 38, 53, 435
BspDI ATCGAT 1 cut(s) 216
BspMAI CTGCAG 1 cut(s) 356
BspPI GGATC 1 cut(s) 482
BspQI GCTCTTC 1 cut(s) 353
BsrDI GCAATG 2 cut(s) 114, 375
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 2 cut(s) 102, 260
BssMI GATC 4 cut(s) 213, 277, 487, 513
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 2 cut(s) 241, 343
Bst6I CTCTTC 2 cut(s) 353, 374
BstDEI CTNAG 4 cut(s) 18, 25, 40, 443
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 2 cut(s) 235, 384
BstF5I GGATG 3 cut(s) 141, 510, 514
BstKTI GATC 4 cut(s) 216, 280, 490, 516
BstMBI GATC 4 cut(s) 213, 277, 487, 513
BstMCI CGRYCG 2 cut(s) 486, 495
BstMWI GCNNNNNNNGC 2 cut(s) 104, 360
BstNI CCWGG 1 cut(s) 261
BstPI GGTNACC 2 cut(s) 235, 384
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 352
BstV1I GCAGC 1 cut(s) 296
BstXI CCANNNNNNTGG 1 cut(s) 109
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 1 cut(s) 259
BsuTUI ATCGAT 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 141, 510, 514
BtsIMutI CAGTG 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 257
ClaI ATCGAT 1 cut(s) 216
CviAII CATG 2 cut(s) 103, 169
DdeI CTNAG 4 cut(s) 18, 25, 40, 443
DpnI GATC 4 cut(s) 215, 279, 489, 515
DpnII GATC 4 cut(s) 213, 277, 487, 513
DraIII CACNNNGTG 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 353, 374
EarI CTCTTC 2 cut(s) 353, 374
Eco130I CCWWGG 1 cut(s) 102
Eco57I CTGAAG 2 cut(s) 16, 461
Eco91I GGTNACC 2 cut(s) 235, 384
EcoO65I GGTNACC 2 cut(s) 235, 384
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 2 cut(s) 106, 172
FaiI YATR 7 cut(s) 104, 170, 273, 315, 421, 423, 471
FatI CATG 2 cut(s) 102, 168
FbaI TGATCA 1 cut(s) 513
Fnu4HI GCNGC 1 cut(s) 285
FokI GGATG 3 cut(s) 148, 517, 521
Fsp4HI GCNGC 1 cut(s) 285
GluI GCNGC 1 cut(s) 285
GsaI CCCAGC 1 cut(s) 441
HaeIII GGCC 1 cut(s) 259
Hin1II CATG 2 cut(s) 106, 172
HinfI GANTC 4 cut(s) 23, 36, 224, 395
HphI GGTGA 3 cut(s) 154, 247, 378
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 3 cut(s) 19, 35, 492
Hpy188III TCNNGA 2 cut(s) 118, 211
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 2 cut(s) 40, 181
HpyCH4III ACNGT 2 cut(s) 241, 343
HpyCH4V TGCA 5 cut(s) 156, 309, 317, 354, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 360
HpyF3I CTNAG 4 cut(s) 18, 25, 40, 443
Hsp92II CATG 2 cut(s) 106, 172
Ksp22I TGATCA 1 cut(s) 513
Kzo9I GATC 4 cut(s) 213, 277, 487, 513
LguI GCTCTTC 1 cut(s) 353
LpnPI CCDG 9 cut(s) 117, 218, 246, 266, 273, 340, 423, 464, 508
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 1 cut(s) 471
MaeIII GTNAC 4 cut(s) 142, 235, 337, 384
MalI GATC 4 cut(s) 215, 279, 489, 515
MboI GATC 4 cut(s) 213, 277, 487, 513
MboII GAAGA 4 cut(s) 196, 361, 364, 370
MhlI GDGCHC 1 cut(s) 58
MluCI AATT 1 cut(s) 414
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 6 cut(s) 13, 214, 222, 298, 438, 439
MroXI GAANNNNTTC 1 cut(s) 164
MslI CAYNNNNRTG 2 cut(s) 107, 173
MspR9I CCNGG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 104, 360
NcoI CCATGG 1 cut(s) 102
NdeII GATC 4 cut(s) 213, 277, 487, 513
NlaIII CATG 2 cut(s) 106, 172
NmuCI GTSAC 4 cut(s) 142, 235, 337, 384
PciSI GCTCTTC 1 cut(s) 353
PctI GAATGC 1 cut(s) 158
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 3 cut(s) 36, 224, 395
PkrI GCNGC 1 cut(s) 286
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 259
PspEI GGTNACC 2 cut(s) 235, 384
PspFI CCCAGC 1 cut(s) 437
PspGI CCWGG 1 cut(s) 259
PspPI GGNCC 1 cut(s) 257
PstI CTGCAG 1 cut(s) 356
RseI CAYNNNNRTG 2 cut(s) 107, 173
SapI GCTCTTC 1 cut(s) 353
SatI GCNGC 1 cut(s) 285
Sau3AI GATC 4 cut(s) 213, 277, 487, 513
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 58
SetI ASST 4 cut(s) 192, 431, 439, 470
SfaNI GCATC 1 cut(s) 471
SfcI CTRYAG 1 cut(s) 352
SmiMI CAYNNNNRTG 2 cut(s) 107, 173
Sse9I AATT 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 2 cut(s) 241, 343
TaqI TCGA 2 cut(s) 216, 227
TaqII GACCGA 2 cut(s) 500, 509
TasI AATT 1 cut(s) 414
TfiI GAWTC 3 cut(s) 36, 224, 395
TscAI CASTG 1 cut(s) 346
TseFI GTSAC 4 cut(s) 142, 235, 337, 384
TseI GCWGC 1 cut(s) 284
Tsp45I GTSAC 4 cut(s) 142, 235, 337, 384
TspDTI ATGAA 5 cut(s) 157, 165, 191, 364, 486
TspRI CASTG 1 cut(s) 346
XapI RAATTY 1 cut(s) 414
XmnI GAANNNNTTC 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.