MD10G1123400.v1.1

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011901
Physical Location & Seq
Forward (+)
20074223 .. 20075862
1640 bp
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UTR
Exon/CDS
Intron
MD10G1123400.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGCAATGGCTTTCAAGATGGCTACAACTGGGATGTGGGTGACTGATGAATGCAAGAACTCATTCATGGAGATGAAATGGAAGAAAGTTCACAGGTACATAGTTTTCAAAATTGATGAGGGATCGAGGCTGGTGACCGTTGACAAAGTTGGTGGGCCAGCGGAAAACTACGATGATCTGGCGGCCTCCTTGCCCAAAGATGATTGCAGATATGCAGTCTTTGATTTTGATTTTGTCACTGTTGATAACTGCAGGAAGAGCAAACTCTTCTTCATTGCTTGGTCACCAAGTGAATCAAGGATTAGGGCAAAAATACTATATGCAACCTCCAAAGCTGGGCTGAGGAGAGTTTTAGATGGCATCAGCTATGAACTTCAGGCGACCGATCCGACCGAGATGGGGATGGATGTGATCAAGGACAGGGCTAAATAA

Protein Analysis

144

Amino Acids

16.34

Weight (kDa)

8.39

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 12 - 142 2.3e-37 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16700 AT2G16700 AT2G16700 AT4G34970
fragaria_vesca FvH4_2g01430
malus_domestica MD05G1119700.v1.1 MD10G1123400.v1.1
prunus_persica Prupe.8G163900_v2.0.a1
pyrus_communis pycom05g11370 pycom10g10570
rosa_chinensis RchiOBHm_Chr6g0244061 RchiOBHm_Chr6g0252411
rosa_laevigata RLG00000015386
rosa_multiflora Rmu_sc0006711.1_g000002
rosa_roxburghii Rroxscaffold_7G00216110
rosa_rugosa Rorug05G0507100
rosa_samantha Rh6AG021500 Rh6BG017200
rosa_wichuraiana Rw6G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 161, 182
AclWI GGATC 2 cut(s) 130, 380
AcuI CTGAAG 1 cut(s) 359
AdeI CACNNNGTG 1 cut(s) 290
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 336, 399
AgsI TTSAA 2 cut(s) 16, 109
AluBI AGCT 2 cut(s) 335, 366
AluI AGCT 2 cut(s) 335, 366
AlwI GGATC 2 cut(s) 130, 380
AoxI GGCC 2 cut(s) 155, 183
Asp700I GAANNNNTTC 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 3 cut(s) 52, 145, 276
BbvCI CCTCAGC 1 cut(s) 341
BccI CCATC 4 cut(s) 13, 350, 391, 397
BclI TGATCA 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 250
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 155
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 1 cut(s) 369
BmuI ACTGGG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 341
Bsc4I CCNNNNNNNGG 2 cut(s) 336, 399
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 2 cut(s) 12, 273
BseGI GGATG 3 cut(s) 39, 408, 412
BseLI CCNNNNNNNGG 2 cut(s) 336, 399
BseMI GCAATG 2 cut(s) 12, 273
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 358
BseYI CCCAGC 1 cut(s) 335
Bsh1285I CGRYCG 2 cut(s) 384, 393
BshFI GGCC 2 cut(s) 157, 185
BsiEI CGRYCG 2 cut(s) 384, 393
BslI CCNNNNNNNGG 2 cut(s) 336, 399
BsmI GAATGC 1 cut(s) 56
BsnI GGCC 2 cut(s) 157, 185
Bsp143I GATC 4 cut(s) 122, 175, 385, 411
BspACI CCGC 2 cut(s) 161, 182
BspANI GGCC 2 cut(s) 157, 185
BspCNI CTCAG 1 cut(s) 333
BspMAI CTGCAG 1 cut(s) 254
BspPI GGATC 2 cut(s) 130, 380
BspQI GCTCTTC 1 cut(s) 251
BsrDI GCAATG 2 cut(s) 12, 273
BsrI ACTGG 1 cut(s) 34
BssMI GATC 4 cut(s) 122, 175, 385, 411
Bst4CI ACNGT 2 cut(s) 139, 241
Bst6I CTCTTC 2 cut(s) 251, 272
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 341
BstEII GGTNACC 2 cut(s) 133, 282
BstF5I GGATG 3 cut(s) 39, 408, 412
BstKTI GATC 4 cut(s) 125, 178, 388, 414
BstMBI GATC 4 cut(s) 122, 175, 385, 411
BstMCI CGRYCG 2 cut(s) 384, 393
BstMWI GCNNNNNNNGC 1 cut(s) 258
BstPI GGTNACC 2 cut(s) 133, 282
BstSFI CTRYAG 1 cut(s) 250
BsuRI GGCC 2 cut(s) 157, 185
BtsCI GGATG 3 cut(s) 39, 408, 412
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 97
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 1 cut(s) 67
CviJI RGCY 9 cut(s) 11, 23, 130, 157, 185, 335, 340, 366, 425
CviKI_1 RGCY 9 cut(s) 11, 23, 130, 157, 185, 335, 340, 366, 425
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 1 cut(s) 341
DpnI GATC 4 cut(s) 124, 177, 387, 413
DpnII GATC 4 cut(s) 122, 175, 385, 411
DraIII CACNNNGTG 1 cut(s) 290
Eam1104I CTCTTC 2 cut(s) 251, 272
EarI CTCTTC 2 cut(s) 251, 272
Eco57I CTGAAG 1 cut(s) 359
Eco91I GGTNACC 2 cut(s) 133, 282
EcoO65I GGTNACC 2 cut(s) 133, 282
FaeI CATG 1 cut(s) 70
FaiI YATR 6 cut(s) 68, 101, 213, 319, 321, 369
FatI CATG 1 cut(s) 66
FbaI TGATCA 1 cut(s) 411
Fnu4HI GCNGC 1 cut(s) 183
FokI GGATG 3 cut(s) 46, 415, 419
Fsp4HI GCNGC 1 cut(s) 183
GluI GCNGC 1 cut(s) 183
GsaI CCCAGC 1 cut(s) 339
HaeIII GGCC 2 cut(s) 157, 185
Hin1II CATG 1 cut(s) 70
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 1 cut(s) 293
HphI GGTGA 3 cut(s) 52, 145, 276
Hpy166II GTNNAC 2 cut(s) 91, 142
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 2 cut(s) 91, 142
HpyCH4III ACNGT 2 cut(s) 139, 241
HpyCH4V TGCA 5 cut(s) 54, 207, 215, 252, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 258
HpyF3I CTNAG 1 cut(s) 341
Hsp92II CATG 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 411
Kzo9I GATC 4 cut(s) 122, 175, 385, 411
LguI GCTCTTC 1 cut(s) 251
LpnPI CCDG 9 cut(s) 15, 79, 116, 164, 171, 238, 321, 362, 406
LweI GCATC 1 cut(s) 369
MaeIII GTNAC 4 cut(s) 40, 133, 235, 282
MalI GATC 4 cut(s) 124, 177, 387, 413
MboI GATC 4 cut(s) 122, 175, 385, 411
MboII GAAGA 4 cut(s) 94, 259, 262, 268
MluCI AATT 1 cut(s) 111
MmeI TCCRAC 1 cut(s) 413
MnlI CCTC 5 cut(s) 112, 120, 196, 336, 337
MroXI GAANNNNTTC 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 161
Mva1269I GAATGC 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 258
NdeII GATC 4 cut(s) 122, 175, 385, 411
NlaIII CATG 1 cut(s) 70
NmuCI GTSAC 4 cut(s) 40, 133, 235, 282
PciSI GCTCTTC 1 cut(s) 251
PctI GAATGC 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 293
PkrI GCNGC 1 cut(s) 184
PspEI GGTNACC 2 cut(s) 133, 282
PspFI CCCAGC 1 cut(s) 335
PspPI GGNCC 1 cut(s) 155
PstI CTGCAG 1 cut(s) 254
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
RseI CAYNNNNRTG 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 251
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 4 cut(s) 122, 175, 385, 411
Sau96I GGNCC 1 cut(s) 155
SetI ASST 4 cut(s) 98, 329, 337, 368
SfaNI GCATC 1 cut(s) 369
SfcI CTRYAG 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 71
Sse9I AATT 1 cut(s) 111
SsiI CCGC 2 cut(s) 161, 182
TaaI ACNGT 2 cut(s) 139, 241
TaqI TCGA 1 cut(s) 125
TaqII GACCGA 2 cut(s) 398, 407
TasI AATT 1 cut(s) 111
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 1 cut(s) 293
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 4 cut(s) 40, 133, 235, 282
Tsp45I GTSAC 4 cut(s) 40, 133, 235, 282
TspDTI ATGAA 5 cut(s) 55, 63, 89, 262, 384
TspRI CASTG 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.