Rh6BG017200

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011901
Physical Location & Seq
Reverse (-)
2487428 .. 2488902
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG017200.1

Sequence Viewer

Length: 399 bp
ATGTGGGTTACTGATGAATGCAAGAACTCATTCCTGGAAATGAAATGGAAGAAGGTGGCTAGGTACATAGTGTACAAGATTGATGAAGAATCGAGGCTTGTGACCGTTGATAAGGTGGGTGGACCTGGTGAAAGCTATGATGATCTTGCAGCTTCTCTGCCCAAAGATGATTGCAGATATGCTGTTTTCGATTTCGATTTCGTCACTGTGGATAATTGCAAGAAGAGCAAAATCTTCTTCATTGCTTGGTCTCCAAGTGAATCAAGGATTAGAGCAAAAATCCTGTATGCAACCTCCAAGGATGGGTTGAGGAGAGTTCTTGATGGCATCAGCTATGAACTTCAGGCTACTGACCCTACTGAGATGGGGATGGATGTGATTAAGGACAGGGCTAAATAA

Protein Analysis

132

Amino Acids

15.18

Weight (kDa)

5.56

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 3 - 131 3.8e-37 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16700 AT2G16700 AT2G16700 AT4G34970
fragaria_vesca FvH4_2g01430
malus_domestica MD05G1119700.v1.1 MD10G1123400.v1.1
prunus_persica Prupe.8G163900_v2.0.a1
pyrus_communis pycom05g11370 pycom10g10570
rosa_chinensis RchiOBHm_Chr6g0244061 RchiOBHm_Chr6g0252411
rosa_laevigata RLG00000015386
rosa_multiflora Rmu_sc0006711.1_g000002
rosa_roxburghii Rroxscaffold_7G00216110
rosa_rugosa Rorug05G0507100
rosa_samantha Rh6AG021500 Rh6BG017200
rosa_wichuraiana Rw6G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 2 cut(s) 65, 74
AfiI CCNNNNNNNGG 1 cut(s) 303
AjnI CCWGG 2 cut(s) 33, 124
AluBI AGCT 3 cut(s) 135, 152, 333
AluI AGCT 3 cut(s) 135, 152, 333
Alw26I GTCTC 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 149
Asp700I GAANNNNTTC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 140
AvaII GGWCC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 161
BccI CCATC 4 cut(s) 296, 317, 358, 364
BciT130I CCWGG 2 cut(s) 35, 126
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 1 cut(s) 60
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 2 cut(s) 35, 126
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 35, 126
BmsI GCATC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 297
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 2 cut(s) 35, 126
BseDI CCNNGG 1 cut(s) 297
BseGI GGATG 3 cut(s) 307, 375, 379
BseLI CCNNNNNNNGG 1 cut(s) 303
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 351
BseRI GAGGAG 1 cut(s) 325
BseXI GCAGC 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 303
BsmAI GTCTC 1 cut(s) 255
BsmI GAATGC 1 cut(s) 23
Bso31I GGTCTC 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 72
Bsp143I GATC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 352
BspQI GCTCTTC 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 297
Bst2UI CCWGG 2 cut(s) 35, 126
Bst4CI ACNGT 2 cut(s) 106, 208
Bst6I CTCTTC 1 cut(s) 218
BstAUI TGTACA 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 360
BstF5I GGATG 3 cut(s) 307, 375, 379
BstKTI GATC 1 cut(s) 145
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstNI CCWGG 2 cut(s) 35, 126
BstSCI CCNGG 2 cut(s) 33, 124
BstV1I GCAGC 1 cut(s) 161
BtsCI GGATG 3 cut(s) 307, 375, 379
BtsIMutI CAGTG 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 122
CsiI ACCWGGT 1 cut(s) 124
Csp6I GTAC 2 cut(s) 64, 73
CviJI RGCY 7 cut(s) 59, 97, 135, 152, 333, 347, 392
CviKI_1 RGCY 7 cut(s) 59, 97, 135, 152, 333, 347, 392
CviQI GTAC 2 cut(s) 64, 73
DdeI CTNAG 1 cut(s) 360
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 33, 124
EcoT14I CCWWGG 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 297
FaiI YATR 5 cut(s) 68, 138, 180, 288, 336
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 3 cut(s) 314, 382, 386
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 60
GluI GCNGC 1 cut(s) 150
HinfI GANTC 2 cut(s) 89, 260
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 2 cut(s) 73, 122
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 2 cut(s) 73, 122
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 2 cut(s) 106, 208
HpyCH4V TGCA 5 cut(s) 21, 149, 174, 219, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpyF3I CTNAG 1 cut(s) 360
Kzo9I GATC 1 cut(s) 142
LguI GCTCTTC 1 cut(s) 218
LpnPI CCDG 7 cut(s) 20, 47, 111, 138, 296, 329, 373
Lsp1109I GCAGC 1 cut(s) 161
LweI GCATC 1 cut(s) 336
MabI ACCWGGT 1 cut(s) 124
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 3 cut(s) 7, 100, 202
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 5 cut(s) 61, 98, 226, 229, 235
MluCI AATT 1 cut(s) 214
MnlI CCTC 3 cut(s) 87, 303, 304
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 1 cut(s) 381
MspR9I CCNGG 2 cut(s) 35, 126
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 2 cut(s) 35, 126
MwoI GCNNNNNNNGC 1 cut(s) 225
NdeII GATC 1 cut(s) 142
NmuCI GTSAC 2 cut(s) 100, 202
PciSI GCTCTTC 1 cut(s) 218
PctI GAATGC 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 2 cut(s) 89, 260
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 1 cut(s) 151
Psp6I CCWGG 2 cut(s) 33, 124
PspGI CCWGG 2 cut(s) 33, 124
PspPI GGNCC 1 cut(s) 122
RsaI GTAC 2 cut(s) 65, 74
RsaNI GTAC 2 cut(s) 64, 73
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 1 cut(s) 142
Sau96I GGNCC 1 cut(s) 122
ScrFI CCNGG 2 cut(s) 35, 126
SetI ASST 8 cut(s) 57, 65, 117, 127, 137, 154, 296, 335
SexAI ACCWGGT 1 cut(s) 124
SfaNI GCATC 1 cut(s) 336
SinI GGWCC 1 cut(s) 122
Sse9I AATT 1 cut(s) 214
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 33, 124
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 2 cut(s) 106, 208
TaqI TCGA 3 cut(s) 92, 189, 195
TasI AATT 1 cut(s) 214
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 2 cut(s) 89, 260
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 2 cut(s) 100, 202
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 2 cut(s) 100, 202
TspDTI ATGAA 5 cut(s) 30, 56, 99, 229, 351
TspRI CASTG 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.