Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006046

Physical Location & Seq

Genomic Coordinates Forward (+)

454777 .. 459696

Gene Structure 4920 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G02250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2412 bp

ATGATGTCGGTGAGAGCAATCAATGGTTGCTCCATCATTCGCACCGCCACTTCCGCCGGCGGTCCTCCCGTCTCTCTATTCCGCCACCGTATTCAGCGCCTCAGAGCTTCTCATCTCCGTGAATTCTCGAAATTACGTTTAAATTTTCCACTAATCCGCGCGGATAGGCGGTTTCTCGGAAATAGTGATGCTCCGAGCTGTTCGACTTGCATACATAGTCTGGTCGAAAGTGTTTCCGAAGAGCTCGAATCCATCAGCAGACGCAAGGGAAGCCGAATGAGAGTTCGCGCCAGTGTAAAGGTGAAATTAACAAGCTACGGAGAGGTTCTAGAGGATAAGCTAGTAAACCAGGAGCTAGAGGCTGGTTTGCTTCTGGAGTTTAAGAAGGACGCAGACAGAGTGTTGTTGGCAGTTCTTCATAGACGTGATGGCAAGAAGAATTGGATGGTGTTTGATCAGAATGGTGTTAGTTGTTCCATCAAACCACAGCAAATTACATACATCGTCCCGAATGTTTATAATTTTGATCATACAGGACTTACTGATTTTCTCCAAAGGGCTCAAGATAACTTGGATCCCCAACTGCTTGAGTTTGCTTGGATGGAGTTACTTGAGAAGAACAAACCTGTGACCCCAGAAGAGTTAGCAGAGATGATATATGGTCGCGCAGATCCTCTTGAAAGCTATTGTGCTCATTTTTTGCTATCACAAGACGAGATATACTTTTCTATCTTGGAGTCAAAAGGTTCTCGATCAATATATAGCCCTCGGCCTACTGAACAGGTGGAAGAGCTTTTACGAAGGCAACGCGTGAAGGAGGCAGAAGATAAAGAGTTTCAGGAGTTTATTCAATTGCTGAAGTCTGCCAAGAAGGCACCTAGTCACGCCAAGCCCCCTAAATCTTCATGGCTAGCTGATGATAAAGTCCAAGATAGGATTGGCTCACTTGAAGCTTATGCCATTGATGCATGGGCAAGCACTGACCAGCAGAAATTGGCTGGAACGATATTAAAATCCATGGGGTTACAGAAAACGTCTGTATCAGCACTAAACCTTCTCATAGATATTGGATATTTTCCTGTACATGTCAATCTTGAACTGCTTAAGCTGAATCTTCCAACCCACCACTCAGAAGCTATCACTGAAGCTGCTGAAGCTCTTCTTTCAGAATCGTCTGATATTGATGCGGTTAGGAGGATTGATCTCACACACTTGAAGGTTTATGCAATTGATGTTGATGAGGCTGATGAGCTTGATGATGCCCTCAGTGCAACGAGATTGCAGGATGGACGGATAAAGATCTGGATACATGTTGCTGATCCTGCTAGATATGTTACACCTGGGAGTAAAGTGGACAGAGAGGCAAGGAGAAGGGGAACTTCTGTCTTTCTGCCAACGGCTACTTATCCAATGTTTCCAGAGAAACTTGCCATGGAAGGAATGAGCTTAAGACAAGGAGAAAACTGTAATGCTGTCTCTGTGTCTGTTGTCCTGCGTTCTGATGGCTGTATTACAGAATATTCAGTAGATAATTCAATCATCAGACCGACCTATATGTTGACGTATGAAAGTGCTTCTGAGCTGCTTCACTTGAACCTAGAAGAAGAAGCTGAACTGAAATTGCTGTCTGAGGCAGCATTCATTCGCTCTCAGTGGCGCCGTGAACAGGGTGCAGTGGACACAACTACACTAGAAACTCGTATCAAAGTGGTGAATCCTGAGGATCCAGAACCTTTGATAAATCTCTATGTAGAAAATCAGGCAGATCTAGCGATGAGACTTGTCTTTGAAATGATGATTCTTTGTGGGGAGGTTGTAGCAACGTTTGGGTCTCAGCATAATATTCCATTGCCATATAGAGGACAGCCACAGTCAAATATTGATGTATCTGCATTTGCGCATCTTCCAGAAGGACCAGTACGTTCCTCATCCATTGTCAAAGTAATGCGTGCTGCAGAAATGAATTTCAGATGTCCTGTTCGACATGGAGTGCTTGGAATTCCTGGTTACGTTCAGTTCACGTCTCCCATTCGTCGATACATGGATCTTACTGCACACTATCAGATTAAAGCTTTTCTTCGAGGAGGTGACAATTTTCCATTCTCTGCTGGTGAGTTAGAAGGTATAGCAGCATCCGTAAACATGCAGAGCAAAGTGGTGAGAAAACTCTCAAACACCGGTCTCCGCTATTGGGTAATAGAGTTTCTCAGAAGACAGGAAAAGGGTAAAAAGTACACCGCATTGGTCTTGAGATTTGTCAAAGACCGGATCGCATCCCTTTTGTTGGTTGAGGTGGGTTTTCAAGCAACGGCGTGGGTGTCAGAAGGAAAACAAGTGGGAGATGAAATCGAAGTTCGAGTAGAAGAAGCTCATCCCCGCGACGATCTTATTCTCTTCAAAGAGGTTATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000178 GO:0000932 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019439 GO:0022613 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035770 GO:0036464 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1905354 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNB

Protein Analysis

803

Amino Acids

90.68

Weight (kDa)

6.2

Isoelectric Point (pI)

42.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CSD_RNase_II	PF23163	119 - 177	1.2e-23	CSD-like barrel domain
WHD_RNase_II	PF25255	182 - 261	8.2e-31	Ribonuclease II, winged helix domain
HTH_RNase_II	PF23161	262 - 365	3.6e-26	Double HTH domain
RNB	PF00773	399 - 694	4e-80	RNB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006046)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02250
fragaria_vesca	FvH4_6g13600 FvH4_6g13600 FvH4_6g13600 FvH4_6g13600 FvH4_6g13600 FvH4_6g13600 FvH4_6g13600
malus_domestica	MD04G1129900.v1.1 MD12G1143000.v1.1
prunus_persica	Prupe.6G256600_v2.0.a1 Prupe.6G256600_v2.0.a1 Prupe.6G256600_v2.0.a1 Prupe.6G256600_v2.0.a1 Prupe.6G256600_v2.0.a1
pyrus_communis	pycom04g11820
rosa_chinensis	RchiOBHm_Chr3g0466451
rosa_laevigata	RLG00000024573
rosa_multiflora	Rmu_sc0036233.1_g000001
rosa_roxburghii	Rroxscaffold_1G00002460 Rroxscaffold_6G00414460
rosa_rugosa	Rorug03G0082000
rosa_samantha	Rh3AG128500 Rh3BG149900 Rh3DG150600
rosa_wichuraiana	Rw3G012140 Rw3G012150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	519
Acc16I	TGCGCA	1 cut(s)	1899
AccB1I	GGYRCC	2 cut(s)	874, 1656
AccII	CGCG	6 cut(s)	159, 161, 288, 666, 810, 2379
AclI	AACGTT	1 cut(s)	1823
AclWI	GGATC	8 cut(s)	569, 582, 665, 1313, 1718, 1731, 2052, 2276
AcsI	RAATTY	4 cut(s)	122, 142, 1963, 1998
AcuI	CTGAAG	3 cut(s)	878, 1164, 1173
AcyI	GRCGYC	1 cut(s)	1657
AfaI	GTAC	3 cut(s)	1083, 1920, 2234
AfiI	CCNNNNNNNGG	4 cut(s)	1666, 1859, 2191, 2284
AflII	CTTAAG	2 cut(s)	1103, 1447
AflIII	ACRYGT	3 cut(s)	808, 1084, 1309
AgeI	ACCGGT	1 cut(s)	2177
AjiI	CACGTC	2 cut(s)	425, 2022
AjnI	CCWGG	3 cut(s)	348, 1339, 2002
Alw21I	GWGCWC	2 cut(s)	246, 694
Alw26I	GTCTC	6 cut(s)	76, 1480, 1771, 1836, 2028, 2186
AlwI	GGATC	8 cut(s)	569, 582, 665, 1313, 1718, 1731, 2052, 2276
AoxI	GGCC	1 cut(s)	770
ApeKI	GCWGC	5 cut(s)	1148, 1582, 1634, 1952, 2129
ApoI	RAATTY	4 cut(s)	122, 142, 1963, 1998
AsiGI	ACCGGT	1 cut(s)	2177
Asp700I	GAANNNNTTC	1 cut(s)	1158
AspLEI	GCGC	6 cut(s)	99, 161, 290, 668, 1659, 1900
AspS9I	GGNCC	2 cut(s)	62, 1913
AsuHPI	GGTGA	6 cut(s)	22, 313, 1723, 2099, 2123, 2170
AsuNHI	GCTAGC	1 cut(s)	910
AvaII	GGWCC	2 cut(s)	62, 1913
AxyI	CCTNAGG	1 cut(s)	1719
BaeI	ACNNNNGTAYC	2 cut(s)	1023, 1056
BamHI	GGATCC	2 cut(s)	574, 1723
BanI	GGYRCC	2 cut(s)	874, 1656
BanII	GRGCYC	2 cut(s)	246, 562
BarI	GAAGNNNNNNTAC	4 cut(s)	780, 812, 1902, 1934
BbsI	GAAGAC	1 cut(s)	2218
Bbv12I	GWGCWC	2 cut(s)	246, 694
BbvI	GCAGC	5 cut(s)	1135, 1569, 1646, 1939, 2141
BccI	CCATC	8 cut(s)	41, 260, 422, 439, 485, 595, 1280, 1496
BceAI	ACGGC	3 cut(s)	1413, 1644, 2325
BciT130I	CCWGG	3 cut(s)	350, 1341, 2004
BciVI	GTATCC	1 cut(s)	1299
BclI	TGATCA	2 cut(s)	454, 526
BcoDI	GTCTC	6 cut(s)	76, 1480, 1771, 1836, 2028, 2186
BfmI	CTRYAG	1 cut(s)	1953
BfoI	RGCGCY	2 cut(s)	100, 1660
BfrI	CTTAAG	2 cut(s)	1103, 1447
BfuI	GTATCC	1 cut(s)	1299
BglI	GCCNNNNNGGC	1 cut(s)	872
BglII	AGATCT	2 cut(s)	1299, 1765
BisI	GCNGC	5 cut(s)	1149, 1583, 1635, 1953, 2130
BlsI	GCNGC	5 cut(s)	1150, 1584, 1636, 1954, 2131
Bme1390I	CCNGG	3 cut(s)	350, 1341, 2004
Bme18I	GGWCC	2 cut(s)	62, 1913
BmgBI	CACGTC	2 cut(s)	425, 2022
BmgT120I	GGNCC	2 cut(s)	62, 1913
BmiI	GGNNCC	4 cut(s)	576, 876, 1658, 1725
BmrFI	CCNGG	3 cut(s)	350, 1341, 2004
BmsI	GCATC	7 cut(s)	178, 955, 1174, 1249, 1909, 2141, 2282
BmtI	GCTAGC	1 cut(s)	914
BpiI	GAAGAC	1 cut(s)	2218
BpmI	CTGGAG	1 cut(s)	395
BpuEI	CTTGAG	4 cut(s)	546, 608, 632, 2269
BsaBI	GATNNNNATC	1 cut(s)	1298
BsaHI	GRCGYC	1 cut(s)	1657
BsaI	GGTCTC	2 cut(s)	1836, 2186
BsaJI	CCNNGG	4 cut(s)	767, 1017, 1340, 1431
BsaWI	WCCGGW	2 cut(s)	2177, 2265
Bsc4I	CCNNNNNNNGG	4 cut(s)	1666, 1859, 2191, 2284
Bse118I	RCCGGY	2 cut(s)	56, 2177
Bse1I	ACTGG	2 cut(s)	291, 1916
Bse21I	CCTNAGG	1 cut(s)	1719
Bse3DI	GCAATG	1 cut(s)	1847
Bse8I	GATNNNNATC	1 cut(s)	1298
BseBI	CCWGG	3 cut(s)	350, 1341, 2004
BseDI	CCNNGG	4 cut(s)	767, 1017, 1340, 1431
BseGI	GGATG	7 cut(s)	450, 606, 1291, 1928, 2132, 2273, 2371
BseJI	GATNNNNATC	1 cut(s)	1298
BseLI	CCNNNNNNNGG	4 cut(s)	1666, 1859, 2191, 2284
BseMI	GCAATG	1 cut(s)	1847
BseMII	CTCAG	9 cut(s)	115, 1143, 1279, 1569, 1620, 1664, 1710, 1847, 2221
BseNI	ACTGG	2 cut(s)	291, 1916
BseRI	GAGGAG	1 cut(s)	2097
BseXI	GCAGC	5 cut(s)	1135, 1569, 1646, 1939, 2141
BsgI	GTGCAG	2 cut(s)	1692, 2037
Bsh1236I	CGCG	6 cut(s)	159, 161, 288, 666, 810, 2379
BshFI	GGCC	1 cut(s)	772
BshNI	GGYRCC	2 cut(s)	874, 1656
BshTI	ACCGGT	1 cut(s)	2177
BsiHKAI	GWGCWC	2 cut(s)	246, 694
BsiSI	CCGG	3 cut(s)	57, 2178, 2266
BslFI	GGGAC	1 cut(s)	491
BslI	CCNNNNNNNGG	4 cut(s)	1666, 1859, 2191, 2284
BsmAI	GTCTC	6 cut(s)	76, 1480, 1771, 1836, 2028, 2186
BsmBI	CGTCTC	2 cut(s)	76, 2028
BsmFI	GGGAC	1 cut(s)	491
BsmI	GAATGC	1 cut(s)	1637
BsnI	GGCC	1 cut(s)	772
Bso31I	GGTCTC	2 cut(s)	1836, 2186
Bsp1286I	GDGCHC	3 cut(s)	246, 562, 694
Bsp1407I	TGTACA	1 cut(s)	1081
Bsp19I	CCATGG	2 cut(s)	1017, 1431
BspANI	GGCC	1 cut(s)	772
BspCNI	CTCAG	9 cut(s)	114, 1142, 1278, 1570, 1621, 1663, 1711, 1846, 2220
BspFNI	CGCG	6 cut(s)	159, 161, 288, 666, 810, 2379
BspLI	GGNNCC	4 cut(s)	576, 876, 1658, 1725
BspMAI	CTGCAG	1 cut(s)	1957
BspOI	GCTAGC	1 cut(s)	914
BspPI	GGATC	8 cut(s)	569, 582, 665, 1313, 1718, 1731, 2052, 2276
BspQI	GCTCTTC	3 cut(s)	234, 783, 1164
BspT107I	GGYRCC	2 cut(s)	874, 1656
BspTI	CTTAAG	2 cut(s)	1103, 1447
BspTNI	GGTCTC	2 cut(s)	1836, 2186
BsrDI	GCAATG	1 cut(s)	1847
BsrFI	RCCGGY	2 cut(s)	56, 2177
BsrGI	TGTACA	1 cut(s)	1081
BsrI	ACTGG	2 cut(s)	291, 1916
BssAI	RCCGGY	2 cut(s)	56, 2177
BssECI	CCNNGG	4 cut(s)	767, 1017, 1340, 1431
BssNI	GRCGYC	1 cut(s)	1657
BssT1I	CCWWGG	2 cut(s)	1017, 1431
Bst2UI	CCWGG	3 cut(s)	350, 1341, 2004
Bst4CI	ACNGT	3 cut(s)	89, 1466, 1872
Bst6I	CTCTTC	5 cut(s)	234, 633, 783, 1164, 2399
BstACI	GRCGYC	1 cut(s)	1657
BstAFI	CTTAAG	2 cut(s)	1103, 1447
BstAUI	TGTACA	1 cut(s)	1081
BstC8I	GCNNGC	4 cut(s)	58, 912, 976, 1950
BstDEI	CTNAG	9 cut(s)	101, 1129, 1265, 1578, 1629, 1650, 1719, 1833, 2207
BstDSI	CCRYGG	2 cut(s)	1017, 1431
BstF5I	GGATG	7 cut(s)	450, 606, 1291, 1928, 2132, 2273, 2371
BstFNI	CGCG	6 cut(s)	159, 161, 288, 666, 810, 2379
BstH2I	RGCGCY	2 cut(s)	100, 1660
BstHHI	GCGC	6 cut(s)	99, 161, 290, 668, 1659, 1900
BstMAI	GTCTC	6 cut(s)	76, 1480, 1771, 1836, 2028, 2186
BstMWI	GCNNNNNNNGC	8 cut(s)	53, 270, 872, 965, 1154, 1268, 1322, 1769
BstNI	CCWGG	3 cut(s)	350, 1341, 2004
BstNSI	RCATGY	3 cut(s)	1088, 1313, 2146
BstSCI	CCNGG	3 cut(s)	348, 1339, 2002
BstSFI	CTRYAG	1 cut(s)	1953
BstUI	CGCG	6 cut(s)	159, 161, 288, 666, 810, 2379
BstV1I	GCAGC	5 cut(s)	1135, 1569, 1646, 1939, 2141
BstV2I	GAAGAC	1 cut(s)	2218
BstX2I	RGATCY	6 cut(s)	574, 670, 1299, 1723, 1765, 2044
BstYI	RGATCY	6 cut(s)	574, 670, 1299, 1723, 1765, 2044
Bsu36I	CCTNAGG	1 cut(s)	1719
BsuI	GTATCC	1 cut(s)	1299
BsuRI	GGCC	1 cut(s)	772
BtgI	CCRYGG	2 cut(s)	1017, 1431
BtgZI	GCGATG	1 cut(s)	1787
BtrI	CACGTC	2 cut(s)	425, 2022
BtsCI	GGATG	7 cut(s)	450, 606, 1291, 1928, 2132, 2273, 2371
BtsI	GCAGTG	1 cut(s)	1680
BtsIMutI	CAGTG	6 cut(s)	298, 978, 1140, 1273, 1658, 1680
Cac8I	GCNNGC	4 cut(s)	58, 912, 976, 1950
CfoI	GCGC	6 cut(s)	99, 161, 290, 668, 1659, 1900
Cfr10I	RCCGGY	2 cut(s)	56, 2177
Cfr13I	GGNCC	2 cut(s)	62, 1913
CseI	GACGC	2 cut(s)	270, 398
Csp6I	GTAC	3 cut(s)	1082, 1919, 2233
CspAI	ACCGGT	1 cut(s)	2177
CviAII	CATG	9 cut(s)	906, 969, 1018, 1085, 1310, 1432, 1985, 2041, 2143
CviQI	GTAC	3 cut(s)	1082, 1919, 2233
DdeI	CTNAG	9 cut(s)	101, 1129, 1265, 1578, 1629, 1650, 1719, 1833, 2207
DinI	GGCGCC	1 cut(s)	1658
DraI	TTTAAA	1 cut(s)	141
Eam1104I	CTCTTC	5 cut(s)	234, 633, 783, 1164, 2399
EarI	CTCTTC	5 cut(s)	234, 633, 783, 1164, 2399
EciI	GGCGGA	2 cut(s)	43, 71
Ecl136II	GAGCTC	1 cut(s)	244
Eco130I	CCWWGG	2 cut(s)	1017, 1431
Eco24I	GRGCYC	2 cut(s)	246, 562
Eco31I	GGTCTC	2 cut(s)	1836, 2186
Eco47I	GGWCC	2 cut(s)	62, 1913
Eco53kI	GAGCTC	1 cut(s)	244
Eco57I	CTGAAG	3 cut(s)	878, 1164, 1173
Eco81I	CCTNAGG	1 cut(s)	1719
EcoICRI	GAGCTC	1 cut(s)	244
EcoRI	GAATTC	2 cut(s)	122, 1998
EcoRII	CCWGG	3 cut(s)	348, 1339, 2002
EcoT14I	CCWWGG	2 cut(s)	1017, 1431
EcoT22I	ATGCAT	1 cut(s)	970
EcoT38I	GRGCYC	2 cut(s)	246, 562
EgeI	GGCGCC	1 cut(s)	1658
EheI	GGCGCC	1 cut(s)	1658
ErhI	CCWWGG	2 cut(s)	1017, 1431
Esp3I	CGTCTC	2 cut(s)	76, 2028
FaeI	CATG	9 cut(s)	909, 972, 1021, 1088, 1313, 1435, 1988, 2044, 2146
FalI	AAGNNNNNCTT	6 cut(s)	1146, 1178, 1363, 1395, 2061, 2093
FaqI	GGGAC	1 cut(s)	491
FatI	CATG	9 cut(s)	905, 968, 1017, 1084, 1309, 1431, 1984, 2040, 2142
FauI	CCCGC	1 cut(s)	2384
FbaI	TGATCA	2 cut(s)	454, 526
Fnu4HI	GCNGC	5 cut(s)	1149, 1583, 1635, 1953, 2130
FokI	GGATG	7 cut(s)	457, 613, 1298, 1915, 2119, 2260, 2358
FriOI	GRGCYC	2 cut(s)	246, 562
Fsp4HI	GCNGC	5 cut(s)	1149, 1583, 1635, 1953, 2130
FspI	TGCGCA	1 cut(s)	1899
GlaI	GCGC	6 cut(s)	98, 160, 289, 667, 1658, 1899
GluI	GCNGC	5 cut(s)	1149, 1583, 1635, 1953, 2130
GsuI	CTGGAG	1 cut(s)	395
HaeII	RGCGCY	2 cut(s)	100, 1660
HaeIII	GGCC	1 cut(s)	772
HapII	CCGG	3 cut(s)	57, 2178, 2266
HgaI	GACGC	2 cut(s)	270, 398
HhaI	GCGC	6 cut(s)	99, 161, 290, 668, 1659, 1900
Hin1I	GRCGYC	1 cut(s)	1657
Hin1II	CATG	9 cut(s)	909, 972, 1021, 1088, 1313, 1435, 1988, 2044, 2146
Hin6I	GCGC	6 cut(s)	97, 159, 288, 666, 1657, 1898
HinP1I	GCGC	6 cut(s)	97, 159, 288, 666, 1657, 1898
HincII	GTYRAC	1 cut(s)	1560
HindII	GTYRAC	1 cut(s)	1560
HindIII	AAGCTT	2 cut(s)	951, 2070
HinfI	GANTC	6 cut(s)	248, 737, 1111, 1169, 1714, 1798
HpaII	CCGG	3 cut(s)	57, 2178, 2266
HphI	GGTGA	6 cut(s)	22, 313, 1723, 2099, 2123, 2170
Hpy166II	GTNNAC	8 cut(s)	346, 1354, 1560, 1664, 1678, 2019, 2140, 2235
Hpy8I	GTNNAC	8 cut(s)	346, 1354, 1560, 1664, 1678, 2019, 2140, 2235
Hpy99I	CGWCG	2 cut(s)	2037, 2384
HpyCH4III	ACNGT	3 cut(s)	89, 1466, 1872
HpyCH4IV	ACGT	8 cut(s)	136, 424, 1034, 1562, 1823, 1921, 2010, 2021
HpyF10VI	GCNNNNNNNGC	8 cut(s)	53, 270, 872, 965, 1154, 1268, 1322, 1769
HpyF3I	CTNAG	9 cut(s)	101, 1129, 1265, 1578, 1629, 1650, 1719, 1833, 2207
HpySE526I	ACGT	8 cut(s)	136, 424, 1034, 1562, 1823, 1921, 2010, 2021
Hsp92I	GRCGYC	1 cut(s)	1657
Hsp92II	CATG	9 cut(s)	909, 972, 1021, 1088, 1313, 1435, 1988, 2044, 2146
HspAI	GCGC	6 cut(s)	97, 159, 288, 666, 1657, 1898
KasI	GGCGCC	1 cut(s)	1656
KroI	GCCGGC	1 cut(s)	56
KroNI	GCCGGC	1 cut(s)	58
Ksp22I	TGATCA	2 cut(s)	454, 526
LguI	GCTCTTC	3 cut(s)	234, 783, 1164
LmnI	GCTCC	3 cut(s)	35, 196, 352
Lsp1109I	GCAGC	5 cut(s)	1135, 1569, 1646, 1939, 2141
LweI	GCATC	7 cut(s)	178, 955, 1174, 1249, 1909, 2141, 2282
MaeII	ACGT	8 cut(s)	136, 424, 1034, 1562, 1823, 1921, 2010, 2021
MaeIII	GTNAC	7 cut(s)	606, 628, 881, 1023, 1333, 2006, 2087
MfeI	CAATTG	2 cut(s)	851, 1227
MflI	RGATCY	6 cut(s)	574, 670, 1299, 1723, 1765, 2044
MhlI	GDGCHC	3 cut(s)	246, 562, 694
MluI	ACGCGT	1 cut(s)	808
Mly113I	GGCGCC	1 cut(s)	1657
MlyI	GAGTC	1 cut(s)	746
MmeI	TCCRAC	1 cut(s)	1142
Mph1103I	ATGCAT	1 cut(s)	970
MreI	CGCCGGCG	1 cut(s)	56
MroNI	GCCGGC	1 cut(s)	56
MroXI	GAANNNNTTC	1 cut(s)	1158
MseI	TTAA	7 cut(s)	140, 308, 381, 1010, 1104, 1448, 2067
MslI	CAYNNNNRTG	2 cut(s)	117, 423
MspCI	CTTAAG	2 cut(s)	1103, 1447
MspI	CCGG	3 cut(s)	57, 2178, 2266
MspR9I	CCNGG	3 cut(s)	350, 1341, 2004
MunI	CAATTG	2 cut(s)	851, 1227
Mva1269I	GAATGC	1 cut(s)	1637
MvaI	CCWGG	3 cut(s)	350, 1341, 2004
MvnI	CGCG	6 cut(s)	159, 161, 288, 666, 810, 2379
MwoI	GCNNNNNNNGC	8 cut(s)	53, 270, 872, 965, 1154, 1268, 1322, 1769
NaeI	GCCGGC	1 cut(s)	58
NarI	GGCGCC	1 cut(s)	1657
NcoI	CCATGG	2 cut(s)	1017, 1431
NgoMIV	GCCGGC	1 cut(s)	56
NheI	GCTAGC	1 cut(s)	910
NlaIII	CATG	9 cut(s)	909, 972, 1021, 1088, 1313, 1435, 1988, 2044, 2146
NlaIV	GGNNCC	4 cut(s)	576, 876, 1658, 1725
NmeAIII	GCCGAG	1 cut(s)	748
NmuCI	GTSAC	3 cut(s)	628, 881, 2087
NsbI	TGCGCA	1 cut(s)	1899
NsiI	ATGCAT	1 cut(s)	970
NspI	RCATGY	3 cut(s)	1088, 1313, 2146
PciI	ACATGT	2 cut(s)	1084, 1309
PciSI	GCTCTTC	3 cut(s)	234, 783, 1164
PctI	GAATGC	1 cut(s)	1637
PdiI	GCCGGC	1 cut(s)	58
PdmI	GAANNNNTTC	1 cut(s)	1158
PfeI	GAWTC	5 cut(s)	248, 1111, 1169, 1714, 1798
PinAI	ACCGGT	1 cut(s)	2177
PkrI	GCNGC	5 cut(s)	1150, 1584, 1636, 1954, 2131
PleI	GAGTC	1 cut(s)	745
PluTI	GGCGCC	1 cut(s)	1660
PpsI	GAGTC	1 cut(s)	745
PscI	ACATGT	2 cut(s)	1084, 1309
PsiI	TTATAA	1 cut(s)	519
Psp124BI	GAGCTC	1 cut(s)	246
Psp1406I	AACGTT	1 cut(s)	1823
Psp6I	CCWGG	3 cut(s)	348, 1339, 2002
PspGI	CCWGG	3 cut(s)	348, 1339, 2002
PspN4I	GGNNCC	4 cut(s)	576, 876, 1658, 1725
PspPI	GGNCC	2 cut(s)	62, 1913
PstI	CTGCAG	1 cut(s)	1957
PsuI	RGATCY	6 cut(s)	574, 670, 1299, 1723, 1765, 2044
RsaI	GTAC	3 cut(s)	1083, 1920, 2234
RsaNI	GTAC	3 cut(s)	1082, 1919, 2233
RseI	CAYNNNNRTG	2 cut(s)	117, 423
SacI	GAGCTC	1 cut(s)	246
SapI	GCTCTTC	3 cut(s)	234, 783, 1164
SaqAI	TTAA	7 cut(s)	140, 308, 381, 1010, 1104, 1448, 2067
SatI	GCNGC	5 cut(s)	1149, 1583, 1635, 1953, 2130
Sau96I	GGNCC	2 cut(s)	62, 1913
SchI	GAGTC	1 cut(s)	746
ScrFI	CCNGG	3 cut(s)	350, 1341, 2004
SduI	GDGCHC	3 cut(s)	246, 562, 694
SfaNI	GCATC	7 cut(s)	178, 955, 1174, 1249, 1909, 2141, 2282
SfcI	CTRYAG	1 cut(s)	1953
SfoI	GGCGCC	1 cut(s)	1658
SgrAI	CRCCGGYG	1 cut(s)	56
SinI	GGWCC	2 cut(s)	62, 1913
SmiMI	CAYNNNNRTG	2 cut(s)	117, 423
SmlI	CTYRAG	6 cut(s)	561, 587, 611, 1103, 1447, 2248
SmoI	CTYRAG	6 cut(s)	561, 587, 611, 1103, 1447, 2248
SspDI	GGCGCC	1 cut(s)	1656
SspI	AATATT	3 cut(s)	1520, 1843, 1879
SstI	GAGCTC	1 cut(s)	246
StyD4I	CCNGG	3 cut(s)	348, 1339, 2002
StyI	CCWWGG	2 cut(s)	1017, 1431
TaaI	ACNGT	3 cut(s)	89, 1466, 1872
TaiI	ACGT	8 cut(s)	139, 427, 1037, 1565, 1826, 1924, 2013, 2024
TaqII	GACCGA	1 cut(s)	1561
TatI	WGTACW	2 cut(s)	1081, 2232
TfiI	GAWTC	5 cut(s)	248, 1111, 1169, 1714, 1798
Tru1I	TTAA	7 cut(s)	140, 308, 381, 1010, 1104, 1448, 2067
Tru9I	TTAA	7 cut(s)	140, 308, 381, 1010, 1104, 1448, 2067
TscAI	CASTG	6 cut(s)	298, 985, 1147, 1273, 1658, 1680
TseFI	GTSAC	3 cut(s)	628, 881, 2087
TseI	GCWGC	5 cut(s)	1148, 1582, 1634, 1952, 2129
Tsp45I	GTSAC	3 cut(s)	628, 881, 2087
TspDTI	ATGAA	6 cut(s)	407, 894, 1581, 1630, 1976, 2358
TspGWI	ACGGA	4 cut(s)	107, 333, 1306, 2125
TspRI	CASTG	6 cut(s)	298, 985, 1147, 1273, 1658, 1680
Vha464I	CTTAAG	2 cut(s)	1103, 1447
VpaK11BI	GGWCC	2 cut(s)	62, 1913
XapI	RAATTY	4 cut(s)	122, 142, 1963, 1998
XbaI	TCTAGA	1 cut(s)	328
XceI	RCATGY	3 cut(s)	1088, 1313, 2146
XcmI	CCANNNNNNNNNTGG	1 cut(s)	935
XmnI	GAANNNNTTC	1 cut(s)	1158
Zsp2I	ATGCAT	1 cut(s)	970

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.