Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006046

Physical Location & Seq

Genomic Coordinates Reverse (-)

22218731 .. 22225511

Gene Structure 6781 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1143000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGGCGGTTCGAGCCACTGTGGGCAGCTGCTCAATCTTCCGCTCCGCCTCCTCTTCCGCTTCCTCCCCCACTCTCCTTCCCTTCCGCTGCTGCCCTTGCCATTTCCCTACTCTCCAGTTCCGCCGACTCTCCAAATTAGGCATTCGCTTCCCGATATTTCGGTCCGGTAAGCTAGCTCCCGGCCATGGCAGCGGCGTTCAAAGCACCTCCGTCCACAGCCTCGTCGATAGCGTCATGGAAGAGCTCGGCGCCCTGCGCAGACGGAGACTAGTTCCTGCTGCCGCCAAGGTGGAGCTGACAAGTAGTGGCGGGATTGTAGAGGATAAGCTTGTGAGCCGGGCACTGCAGCAAGGGTTGCTGCTTGAATTCAAGAAGGATGCAGAGAGAGTGTTGCTGGCTGTTGCGCAGAAGCCCGATGGCAAGAAGAACTGGATGGTGTCCGATCAGAATGGTGTTACATCGTCGATTAAACCACAACAGATTACGTATATTGTTCCCGGTGTTGAGAATTTTGACCACACAGATATATCAGACTTTATTCAGAAAGCTCAAGCTAACTCGGATCCAGCACTGCTTGAATTTGCTTGGGTTGAGCTCCTTGAAAAGAATAAGCGGGTGACGGCAGAAGAATTAGCTGAGATGATATTTGGTAGTGTAGAGCCTCTTGAATGCTACTGTGCTCATCTGTTGCTTTCAGAAGATGAAGTATACTTCACTGTTCTGGAGACAAAAGGTTCTCGCTCTGTATATGGACCTCGACCTGCTGTACAGGTTGAGGAGCTCCTGCGTAGGAAGCTCGCAAAGGAGGCTGCTGAGAAAGAACTGCAGGAGTTTAAACAATTACTGAAATCTGCTAAGGCAATGCCTTTAAATGCTAAACCTCCCAAATCTTCTTGGTTGGCTGAAGAGAAAATCAGACACAAAATTGAGTCTCTTGAAGCTTATGCTATTGATGCTTGCACAAATGATGACCAAAGGAAAACAGCCGGAACAATCCTAAGAACAATGGGGATGGTAAAAACAGCCTCATCAGCTTTGAATCTCCTCATAGACATTGGGTATTTTCCTGTACACGTGAATCTTGACCTGTTGAAGTTGAACATCCGTACTGATCATTCAGATGGAGTTATATCAGCTGCAGAACATCTTCTGTCAGATTCATCTGATTTAGATGTGATTGAAAGGACAGATCTCACTCACTTGAAGGTCTATGCTATTGATGTGGATGAGGCTGATGAGCTTGATGATGCCCTGAGTGCAACGAGGCTACAAGATGGACGCATTAAAATATGGATTCATGTTGCAGATGCAACTAGGTTTGTCCAACCTGGGAGCATAGTAGACAGGGAGGCAATGAGAAGAGGAACTTCTGTATTCTTGCCCACTGCTACTTATCCTATGTTCCCGGAGAAACTTGCCATGGAGGGAATGAGTTTGCAACAAGGAGAGATTTGCAATGCGGTTACTGTATCTGTGGTGCTACACTCCGATGGAAGTATTGCAGAATATTCAGTGGACAACTCAATCATTAAACCAACATACATGTTGACATATGAGAGTGCATCTGAGCTTCTTCATTTGAACCTGCAAGAAGAGGCTGAACTGAAAATCCTGTCTGAGGCTGCAACTCTCCGGTCAATATGGCGTCGAGAACAGGGCGCAATTGACACAGCCACATTGGAAGCACGCATCAAAGTGGTTAATCCAGAGGATCCGGAGCCTGTGATTAATTTGTATGTAGAAAACCAGGCAGACCCTGCAATGCGACTTGTCACTGAGATGATGGTACTCTGCGGAGAAGTTATAGCCACTTTTGGTTCTAGAAATAACATTCCTTTACCCTACAGAGGACAACCCCAGTCCAACATCGACACCTCTGTATATGCACATCTTCCAGAAGGACCTGTTAGAAGCTCTGCTCTTGTTAAACTAATGCGTGCTGCTGAAATTGATTTCAGAAAACCTATACGTCATGGAATTTTAGGACTTCCGGGTTATGTTCAGTTTACATCTCCCATCCGTAGATATATGGATCTACTAGCTCACTATCAGGTGAAATCATTTCTCGCAGGGGAATCTCTTCCTTTCTCAGCTGGTCAGTTGGAGGGGATGGCATCTATTGTGAACATGAATGTTAGAGTAGCAAGGAGGCTCTTTAGCAGCAGTCTTCGCTACTGGATATTAGAATATCTGAGAAGGCAGCCAAAAGAAAAAAAATATCGGGCGCTGATTCTTAGATTCATTAAGGATCGGATTGCAGCCATATTGTTAGTTGAGGTGGGACTTCAATCTTCCGTGTGGATATCCGTGGGAGCCCAAATTGGAGATGAAGTTCTTGTTCGGATAGAAGAAGCTCATCCACGGGATGATGTGCTTTTACTTAAAGAGGTTGTCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000178 GO:0000932 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019439 GO:0022613 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035770 GO:0036464 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1905354 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNB

Protein Analysis

800

Amino Acids

88.93

Weight (kDa)

6.17

Isoelectric Point (pI)

48.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CSD_RNase_II	PF23163	115 - 176	2.3e-27	CSD-like barrel domain
WHD_RNase_II	PF25255	178 - 257	4.7e-31	Ribonuclease II, winged helix domain
HTH_RNase_II	PF23161	258 - 361	4.3e-27	Double HTH domain
RNB	PF00773	395 - 690	2.5e-75	RNB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006046)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02250
fragaria_vesca	FvH4_6g13600 FvH4_6g13600 FvH4_6g13600 FvH4_6g13600 FvH4_6g13600 FvH4_6g13600 FvH4_6g13600
malus_domestica	MD04G1129900.v1.1 MD12G1143000.v1.1
prunus_persica	Prupe.6G256600_v2.0.a1 Prupe.6G256600_v2.0.a1 Prupe.6G256600_v2.0.a1 Prupe.6G256600_v2.0.a1 Prupe.6G256600_v2.0.a1
pyrus_communis	pycom04g11820
rosa_chinensis	RchiOBHm_Chr3g0466451
rosa_laevigata	RLG00000024573
rosa_multiflora	Rmu_sc0036233.1_g000001
rosa_roxburghii	Rroxscaffold_1G00002460 Rroxscaffold_6G00414460
rosa_rugosa	Rorug03G0082000
rosa_samantha	Rh3AG128500 Rh3BG149900 Rh3DG150600
rosa_wichuraiana	Rw3G012140 Rw3G012150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	257, 405
Acc36I	ACCTGC	2 cut(s)	769, 1593
AccB1I	GGYRCC	1 cut(s)	248
AccBSI	CCGCTC	1 cut(s)	42
AccI	GTMKAC	2 cut(s)	708, 1341
AccIII	TCCGGA	1 cut(s)	1714
AclWI	GGATC	6 cut(s)	557, 570, 1706, 1719, 2040, 2256
AcoI	YGGCCR	1 cut(s)	181
AcsI	RAATTY	4 cut(s)	365, 508, 578, 1977
AcuI	CTGAAG	1 cut(s)	924
AcvI	CACGTG	1 cut(s)	1075
AcyI	GRCGYC	2 cut(s)	249, 1645
AfaI	GTAC	4 cut(s)	768, 1071, 1108, 1788
AfiI	CCNNNNNNNGG	3 cut(s)	185, 1618, 1847
AflIII	ACRYGT	2 cut(s)	1072, 1542
AhlI	ACTAGT	1 cut(s)	268
AjnI	CCWGG	2 cut(s)	1327, 1746
AloI	GAACNNNNNNTCC	4 cut(s)	1593, 1625, 2316, 2348
Alw21I	GWGCWC	4 cut(s)	246, 597, 682, 783
Alw26I	GTCTC	3 cut(s)	259, 719, 936
AlwI	GGATC	6 cut(s)	557, 570, 1706, 1719, 2040, 2256
AlwNI	CAGNNNCTG	1 cut(s)	1757
Aor13HI	TCCGGA	1 cut(s)	1714
AoxI	GGCC	1 cut(s)	181
ApoI	RAATTY	4 cut(s)	365, 508, 578, 1977
AseI	ATTAAT	1 cut(s)	1728
Asp700I	GAANNNNTTC	2 cut(s)	1146, 2079
AspLEI	GCGC	5 cut(s)	251, 258, 406, 1661, 2227
AspS9I	GGNCC	3 cut(s)	162, 752, 1901
AsuC2I	CCSGG	5 cut(s)	180, 338, 498, 1406, 1992
AsuHPI	GGTGA	2 cut(s)	628, 2065
AsuNHI	GCTAGC	1 cut(s)	172
AvaII	GGWCC	3 cut(s)	162, 752, 1901
BaeGI	GKGCMC	1 cut(s)	343
BamHI	GGATCC	2 cut(s)	562, 1711
BanI	GGYRCC	1 cut(s)	248
BanII	GRGCYC	4 cut(s)	246, 597, 783, 2317
BbrPI	CACGTG	1 cut(s)	1075
BbsI	GAAGAC	1 cut(s)	2159
Bbv12I	GWGCWC	4 cut(s)	246, 597, 682, 783
BccI	CCATC	9 cut(s)	410, 427, 1006, 1115, 1268, 1484, 1777, 2024, 2104
BceAI	ACGGC	1 cut(s)	636
BciT130I	CCWGG	2 cut(s)	1329, 1748
BclI	TGATCA	1 cut(s)	1111
BcnI	CCSGG	5 cut(s)	180, 338, 498, 1406, 1992
BcoDI	GTCTC	3 cut(s)	259, 719, 936
BcuI	ACTAGT	1 cut(s)	268
BfaI	CTAG	5 cut(s)	173, 269, 1314, 1821, 2039
BfmI	CTRYAG	4 cut(s)	344, 824, 1137, 1843
BfoI	RGCGCY	2 cut(s)	252, 2228
BfuAI	ACCTGC	2 cut(s)	769, 1593
BglII	AGATCT	1 cut(s)	1189
Bme1390I	CCNGG	7 cut(s)	180, 338, 498, 1329, 1406, 1748, 1992
Bme18I	GGWCC	3 cut(s)	162, 752, 1901
BmgT120I	GGNCC	3 cut(s)	162, 752, 1901
BmiI	GGNNCC	5 cut(s)	250, 564, 1713, 1719, 2314
BmrFI	CCNGG	7 cut(s)	180, 338, 498, 1329, 1406, 1748, 1992
BmrI	ACTGGG	1 cut(s)	1852
BmsI	GCATC	7 cut(s)	367, 943, 1237, 1297, 1571, 1698, 2123
BmtI	GCTAGC	1 cut(s)	176
BmuI	ACTGGG	1 cut(s)	1852
BpiI	GAAGAC	1 cut(s)	2159
BpmI	CTGGAG	2 cut(s)	98, 743
Bpu10I	CCTNAGC	1 cut(s)	855
BpuEI	CTTGAG	1 cut(s)	534
BpuMI	CCSGG	5 cut(s)	180, 338, 498, 1406, 1992
BsaAI	YACGTR	2 cut(s)	486, 1075
BsaHI	GRCGYC	2 cut(s)	249, 1645
BsaJI	CCNNGG	6 cut(s)	184, 285, 1328, 1419, 2307, 2360
BsaWI	WCCGGW	3 cut(s)	164, 1632, 1714
BsaXI	ACNNNNNCTCC	2 cut(s)	2316, 2346
Bsc4I	CCNNNNNNNGG	3 cut(s)	185, 1618, 1847
Bse1I	ACTGG	4 cut(s)	115, 434, 1858, 2180
Bse3DI	GCAATG	4 cut(s)	867, 1359, 1462, 1767
BseAI	TCCGGA	1 cut(s)	1714
BseBI	CCWGG	2 cut(s)	1329, 1748
BseDI	CCNNGG	6 cut(s)	184, 285, 1328, 1419, 2307, 2360
BseGI	GGATG	9 cut(s)	382, 438, 1017, 1101, 1231, 2016, 2115, 2356, 2371
BseLI	CCNNNNNNNGG	3 cut(s)	185, 1618, 1847
BseMI	GCAATG	4 cut(s)	867, 1359, 1462, 1767
BseMII	CTCAG	8 cut(s)	627, 804, 1244, 1557, 1608, 1767, 2103, 2183
BseNI	ACTGG	4 cut(s)	115, 434, 1858, 2180
BseRI	GAGGAG	3 cut(s)	40, 791, 1034
BseSI	GKGCMC	1 cut(s)	343
BshFI	GGCC	1 cut(s)	183
BshNI	GGYRCC	1 cut(s)	248
BsiHKAI	GWGCWC	4 cut(s)	246, 597, 682, 783
BsiSI	CCGG	9 cut(s)	165, 180, 337, 498, 987, 1406, 1633, 1715, 1991
BslFI	GGGAC	1 cut(s)	2295
BslI	CCNNNNNNNGG	3 cut(s)	185, 1618, 1847
BsmAI	GTCTC	3 cut(s)	259, 719, 936
BsmFI	GGGAC	1 cut(s)	2295
BsmI	GAATGC	2 cut(s)	141, 674
BsnI	GGCC	1 cut(s)	183
Bsp1286I	GDGCHC	6 cut(s)	246, 343, 597, 682, 783, 2317
Bsp13I	TCCGGA	1 cut(s)	1714
Bsp1407I	TGTACA	2 cut(s)	766, 1069
Bsp143I	GATC	7 cut(s)	442, 562, 1111, 1189, 1711, 2032, 2248
Bsp19I	CCATGG	2 cut(s)	184, 1419
BspANI	GGCC	1 cut(s)	183
BspCNI	CTCAG	8 cut(s)	628, 805, 1245, 1558, 1609, 1768, 2102, 2184
BspEI	TCCGGA	1 cut(s)	1714
BspLI	GGNNCC	5 cut(s)	250, 564, 1713, 1719, 2314
BspMAI	CTGCAG	3 cut(s)	348, 828, 1141
BspMI	ACCTGC	2 cut(s)	769, 1593
BspOI	GCTAGC	1 cut(s)	176
BspPI	GGATC	6 cut(s)	557, 570, 1706, 1719, 2040, 2256
BspQI	GCTCTTC	1 cut(s)	234
BspT107I	GGYRCC	1 cut(s)	248
BsrBI	CCGCTC	1 cut(s)	42
BsrDI	GCAATG	4 cut(s)	867, 1359, 1462, 1767
BsrGI	TGTACA	2 cut(s)	766, 1069
BsrI	ACTGG	4 cut(s)	115, 434, 1858, 2180
BssECI	CCNNGG	6 cut(s)	184, 285, 1328, 1419, 2307, 2360
BssMI	GATC	7 cut(s)	442, 562, 1111, 1189, 1711, 2032, 2248
BssNAI	GTATAC	1 cut(s)	709
BssNI	GRCGYC	2 cut(s)	249, 1645
BssT1I	CCWWGG	3 cut(s)	184, 285, 1419
Bst1107I	GTATAC	1 cut(s)	709
Bst2UI	CCWGG	2 cut(s)	1329, 1748
Bst4CI	ACNGT	4 cut(s)	19, 677, 718, 1468
Bst6I	CTCTTC	6 cut(s)	58, 234, 900, 1354, 1587, 2085
BstACI	GRCGYC	2 cut(s)	249, 1645
BstAPI	GCANNNNNTGC	2 cut(s)	355, 1757
BstAUI	TGTACA	2 cut(s)	766, 1069
BstBAI	YACGTR	2 cut(s)	486, 1075
BstC8I	GCNNGC	6 cut(s)	174, 396, 798, 958, 1687, 1938
BstDSI	CCRYGG	4 cut(s)	184, 1419, 2307, 2360
BstENI	CCTNNNNNAGG	1 cut(s)	1616
BstF5I	GGATG	9 cut(s)	382, 438, 1017, 1101, 1231, 2016, 2115, 2356, 2371
BstH2I	RGCGCY	2 cut(s)	252, 2228
BstHHI	GCGC	5 cut(s)	251, 258, 406, 1661, 2227
BstKTI	GATC	7 cut(s)	445, 565, 1114, 1192, 1714, 2035, 2251
BstMAI	GTCTC	3 cut(s)	259, 719, 936
BstMBI	GATC	7 cut(s)	442, 562, 1111, 1189, 1711, 2032, 2248
BstNI	CCWGG	2 cut(s)	1329, 1748
BstNSI	RCATGY	1 cut(s)	1546
BstSCI	CCNGG	7 cut(s)	178, 336, 496, 1327, 1404, 1746, 1990
BstSFI	CTRYAG	4 cut(s)	344, 824, 1137, 1843
BstSLI	GKGCMC	1 cut(s)	343
BstSNI	TACGTA	1 cut(s)	486
BstV2I	GAAGAC	1 cut(s)	2159
BstX2I	RGATCY	4 cut(s)	562, 1189, 1711, 2032
BstYI	RGATCY	4 cut(s)	562, 1189, 1711, 2032
BstZ17I	GTATAC	1 cut(s)	709
BsuRI	GGCC	1 cut(s)	183
BtgI	CCRYGG	4 cut(s)	184, 1419, 2307, 2360
BtsCI	GGATG	9 cut(s)	382, 438, 1017, 1101, 1231, 2016, 2115, 2356, 2371
BtsI	GCAGTG	3 cut(s)	341, 569, 1383
BtsIMutI	CAGTG	7 cut(s)	15, 341, 569, 714, 1383, 1518, 1773
BveI	ACCTGC	2 cut(s)	769, 1593
Cac8I	GCNNGC	6 cut(s)	174, 396, 798, 958, 1687, 1938
CaiI	CAGNNNCTG	1 cut(s)	1757
CfoI	GCGC	5 cut(s)	251, 258, 406, 1661, 2227
Cfr13I	GGNCC	3 cut(s)	162, 752, 1901
CpoI	CGGWCCG	1 cut(s)	162
CseI	GACGC	3 cut(s)	220, 1287, 1634
Csp6I	GTAC	4 cut(s)	767, 1070, 1107, 1787
CspI	CGGWCCG	1 cut(s)	162
CviAII	CATG	7 cut(s)	185, 235, 1298, 1420, 1543, 1973, 2128
CviQI	GTAC	4 cut(s)	767, 1070, 1107, 1787
DinI	GGCGCC	1 cut(s)	250
DpnI	GATC	7 cut(s)	444, 564, 1113, 1191, 1713, 2034, 2250
DpnII	GATC	7 cut(s)	442, 562, 1111, 1189, 1711, 2032, 2248
DraI	TTTAAA	2 cut(s)	835, 870
EaeI	YGGCCR	1 cut(s)	181
Eam1104I	CTCTTC	6 cut(s)	58, 234, 900, 1354, 1587, 2085
EarI	CTCTTC	6 cut(s)	58, 234, 900, 1354, 1587, 2085
EciI	GGCGGA	2 cut(s)	34, 110
Ecl136II	GAGCTC	3 cut(s)	244, 595, 781
Eco105I	TACGTA	1 cut(s)	486
Eco130I	CCWWGG	3 cut(s)	184, 285, 1419
Eco24I	GRGCYC	4 cut(s)	246, 597, 783, 2317
Eco32I	GATATC	1 cut(s)	2304
Eco47I	GGWCC	3 cut(s)	162, 752, 1901
Eco53kI	GAGCTC	3 cut(s)	244, 595, 781
Eco57I	CTGAAG	1 cut(s)	924
Eco72I	CACGTG	1 cut(s)	1075
EcoICRI	GAGCTC	3 cut(s)	244, 595, 781
EcoNI	CCTNNNNNAGG	1 cut(s)	1616
EcoO109I	RGGNCCY	1 cut(s)	1901
EcoRI	GAATTC	1 cut(s)	365
EcoRII	CCWGG	2 cut(s)	1327, 1746
EcoRV	GATATC	1 cut(s)	2304
EcoT14I	CCWWGG	3 cut(s)	184, 285, 1419
EcoT38I	GRGCYC	4 cut(s)	246, 597, 783, 2317
EgeI	GGCGCC	1 cut(s)	250
EheI	GGCGCC	1 cut(s)	250
ErhI	CCWWGG	3 cut(s)	184, 285, 1419
FaeI	CATG	7 cut(s)	188, 238, 1301, 1423, 1546, 1976, 2131
FalI	AAGNNNNNCTT	2 cut(s)	1351, 1383
FaqI	GGGAC	1 cut(s)	2295
FatI	CATG	7 cut(s)	184, 234, 1297, 1419, 1542, 1972, 2127
FauI	CCCGC	2 cut(s)	302, 606
FauNDI	CATATG	1 cut(s)	1552
FbaI	TGATCA	1 cut(s)	1111
FblI	GTMKAC	2 cut(s)	708, 1341
FokI	GGATG	9 cut(s)	389, 445, 1024, 1088, 1238, 2003, 2122, 2343, 2378
FriOI	GRGCYC	4 cut(s)	246, 597, 783, 2317
FspBI	CTAG	5 cut(s)	173, 269, 1314, 1821, 2039
FspI	TGCGCA	2 cut(s)	257, 405
GlaI	GCGC	5 cut(s)	250, 257, 405, 1660, 2226
GsuI	CTGGAG	2 cut(s)	98, 743
HaeII	RGCGCY	2 cut(s)	252, 2228
HaeIII	GGCC	1 cut(s)	183
HapII	CCGG	9 cut(s)	165, 180, 337, 498, 987, 1406, 1633, 1715, 1991
HgaI	GACGC	3 cut(s)	220, 1287, 1634
HhaI	GCGC	5 cut(s)	251, 258, 406, 1661, 2227
Hin1I	GRCGYC	2 cut(s)	249, 1645
Hin1II	CATG	7 cut(s)	188, 238, 1301, 1423, 1546, 1976, 2131
Hin6I	GCGC	5 cut(s)	249, 256, 404, 1659, 2225
HinP1I	GCGC	5 cut(s)	249, 256, 404, 1659, 2225
HincII	GTYRAC	1 cut(s)	1548
HindII	GTYRAC	1 cut(s)	1548
HindIII	AAGCTT	2 cut(s)	326, 939
HinfI	GANTC	9 cut(s)	126, 929, 1039, 1078, 1157, 1294, 2075, 2230, 2238
HpaII	CCGG	9 cut(s)	165, 180, 337, 498, 987, 1406, 1633, 1715, 1991
HphI	GGTGA	2 cut(s)	628, 2065
Hpy166II	GTNNAC	8 cut(s)	214, 709, 1072, 1342, 1516, 1548, 2007, 2125
Hpy8I	GTNNAC	8 cut(s)	214, 709, 1072, 1342, 1516, 1548, 2007, 2125
Hpy99I	CGWCG	3 cut(s)	227, 466, 1650
HpyAV	CCTTC	6 cut(s)	86, 91, 367, 1198, 1892, 2190
HpyCH4III	ACNGT	4 cut(s)	19, 677, 718, 1468
HpyCH4IV	ACGT	3 cut(s)	485, 1074, 1969
HpySE526I	ACGT	3 cut(s)	485, 1074, 1969
Hsp92I	GRCGYC	2 cut(s)	249, 1645
Hsp92II	CATG	7 cut(s)	188, 238, 1301, 1423, 1546, 1976, 2131
HspAI	GCGC	5 cut(s)	249, 256, 404, 1659, 2225
KasI	GGCGCC	1 cut(s)	248
Kpn2I	TCCGGA	1 cut(s)	1714
Ksp22I	TGATCA	1 cut(s)	1111
Kzo9I	GATC	7 cut(s)	442, 562, 1111, 1189, 1711, 2032, 2248
LguI	GCTCTTC	1 cut(s)	234
LmnI	GCTCC	9 cut(s)	47, 181, 292, 600, 778, 786, 1332, 1717, 2312
LweI	GCATC	7 cut(s)	367, 943, 1237, 1297, 1571, 1698, 2123
MaeI	CTAG	5 cut(s)	173, 269, 1314, 1821, 2039
MaeII	ACGT	3 cut(s)	485, 1074, 1969
MaeIII	GTNAC	4 cut(s)	454, 616, 1462, 1771
MalI	GATC	7 cut(s)	444, 564, 1113, 1191, 1713, 2034, 2250
MbiI	CCGCTC	1 cut(s)	42
MboI	GATC	7 cut(s)	442, 562, 1111, 1189, 1711, 2032, 2248
MfeI	CAATTG	1 cut(s)	1662
MflI	RGATCY	4 cut(s)	562, 1189, 1711, 2032
MhlI	GDGCHC	6 cut(s)	246, 343, 597, 682, 783, 2317
Mly113I	GGCGCC	1 cut(s)	249
MlyI	GAGTC	2 cut(s)	120, 938
MmeI	TCCRAC	3 cut(s)	1348, 1887, 2082
MroI	TCCGGA	1 cut(s)	1714
MroXI	GAANNNNTTC	2 cut(s)	1146, 2079
MslI	CAYNNNNRTG	4 cut(s)	1119, 1488, 1694, 1778
MspA1I	CMGCKG	5 cut(s)	27, 87, 192, 1136, 2093
MspI	CCGG	9 cut(s)	165, 180, 337, 498, 987, 1406, 1633, 1715, 1991
MspR9I	CCNGG	7 cut(s)	180, 338, 498, 1329, 1406, 1748, 1992
MssI	GTTTAAAC	1 cut(s)	835
MunI	CAATTG	1 cut(s)	1662
Mva1269I	GAATGC	2 cut(s)	141, 674
MvaI	CCWGG	2 cut(s)	1329, 1748
NarI	GGCGCC	1 cut(s)	249
NciI	CCSGG	5 cut(s)	180, 338, 498, 1406, 1992
NcoI	CCATGG	2 cut(s)	184, 1419
NdeI	CATATG	1 cut(s)	1552
NdeII	GATC	7 cut(s)	442, 562, 1111, 1189, 1711, 2032, 2248
NheI	GCTAGC	1 cut(s)	172
NlaIII	CATG	7 cut(s)	188, 238, 1301, 1423, 1546, 1976, 2131
NlaIV	GGNNCC	5 cut(s)	250, 564, 1713, 1719, 2314
NmeAIII	GCCGAG	1 cut(s)	225
NmuCI	GTSAC	2 cut(s)	616, 1771
NsbI	TGCGCA	2 cut(s)	257, 405
NspI	RCATGY	1 cut(s)	1546
PciI	ACATGT	1 cut(s)	1542
PciSI	GCTCTTC	1 cut(s)	234
PcsI	WCGNNNNNNNCGW	1 cut(s)	228
PctI	GAATGC	2 cut(s)	141, 674
PdmI	GAANNNNTTC	2 cut(s)	1146, 2079
PfeI	GAWTC	7 cut(s)	1039, 1078, 1157, 1294, 2075, 2230, 2238
PfoI	TCCNGGA	1 cut(s)	1404
PleI	GAGTC	2 cut(s)	120, 937
PluTI	GGCGCC	1 cut(s)	252
PmaCI	CACGTG	1 cut(s)	1075
PmeI	GTTTAAAC	1 cut(s)	835
PmlI	CACGTG	1 cut(s)	1075
PpsI	GAGTC	2 cut(s)	120, 937
Ppu21I	YACGTR	2 cut(s)	486, 1075
PpuMI	RGGWCCY	1 cut(s)	1901
PscI	ACATGT	1 cut(s)	1542
PshBI	ATTAAT	1 cut(s)	1728
Psp124BI	GAGCTC	3 cut(s)	246, 597, 783
Psp5II	RGGWCCY	1 cut(s)	1901
Psp6I	CCWGG	2 cut(s)	1327, 1746
PspCI	CACGTG	1 cut(s)	1075
PspGI	CCWGG	2 cut(s)	1327, 1746
PspN4I	GGNNCC	5 cut(s)	250, 564, 1713, 1719, 2314
PspPI	GGNCC	3 cut(s)	162, 752, 1901
PspPPI	RGGWCCY	1 cut(s)	1901
PstI	CTGCAG	3 cut(s)	348, 828, 1141
PstNI	CAGNNNCTG	1 cut(s)	1757
PsuI	RGATCY	4 cut(s)	562, 1189, 1711, 2032
PvuII	CAGCTG	3 cut(s)	27, 1136, 2093
RsaI	GTAC	4 cut(s)	768, 1071, 1108, 1788
RsaNI	GTAC	4 cut(s)	767, 1070, 1107, 1787
RseI	CAYNNNNRTG	4 cut(s)	1119, 1488, 1694, 1778
Rsr2I	CGGWCCG	1 cut(s)	162
RsrII	CGGWCCG	1 cut(s)	162
SacI	GAGCTC	3 cut(s)	246, 597, 783
SapI	GCTCTTC	1 cut(s)	234
Sau3AI	GATC	7 cut(s)	442, 562, 1111, 1189, 1711, 2032, 2248
Sau96I	GGNCC	3 cut(s)	162, 752, 1901
SchI	GAGTC	2 cut(s)	120, 938
ScrFI	CCNGG	7 cut(s)	180, 338, 498, 1329, 1406, 1748, 1992
SduI	GDGCHC	6 cut(s)	246, 343, 597, 682, 783, 2317
SfaNI	GCATC	7 cut(s)	367, 943, 1237, 1297, 1571, 1698, 2123
SfcI	CTRYAG	4 cut(s)	344, 824, 1137, 1843
SfoI	GGCGCC	1 cut(s)	250
SinI	GGWCC	3 cut(s)	162, 752, 1901
SmiMI	CAYNNNNRTG	4 cut(s)	1119, 1488, 1694, 1778
SmlI	CTYRAG	1 cut(s)	549
SmoI	CTYRAG	1 cut(s)	549
SnaBI	TACGTA	1 cut(s)	486
SpeI	ACTAGT	1 cut(s)	268
SspDI	GGCGCC	1 cut(s)	248
SspI	AATATT	1 cut(s)	1508
SspMI	CTAG	5 cut(s)	173, 269, 1314, 1821, 2039
SstI	GAGCTC	3 cut(s)	246, 597, 783
StyD4I	CCNGG	7 cut(s)	178, 336, 496, 1327, 1404, 1746, 1990
StyI	CCWWGG	3 cut(s)	184, 285, 1419
TaaI	ACNGT	4 cut(s)	19, 677, 718, 1468
TaiI	ACGT	3 cut(s)	488, 1077, 1972
TaqI	TCGA	6 cut(s)	10, 225, 464, 757, 1648, 1869
TaqII	GACCGA	1 cut(s)	150
TatI	WGTACW	2 cut(s)	766, 1069
TauI	GCSGC	2 cut(s)	195, 284
TfiI	GAWTC	7 cut(s)	1039, 1078, 1157, 1294, 2075, 2230, 2238
TscAI	CASTG	7 cut(s)	22, 348, 576, 721, 1390, 1518, 1780
TseFI	GTSAC	2 cut(s)	616, 1771
Tsp45I	GTSAC	2 cut(s)	616, 1771
TspDTI	ATGAA	7 cut(s)	717, 1149, 1286, 1565, 2144, 2230, 2343
TspGWI	ACGGA	6 cut(s)	199, 277, 1094, 2009, 2284, 2296
TspRI	CASTG	7 cut(s)	22, 348, 576, 721, 1390, 1518, 1780
VpaK11BI	GGWCC	3 cut(s)	162, 752, 1901
VspI	ATTAAT	1 cut(s)	1728
XagI	CCTNNNNNAGG	1 cut(s)	1616
XapI	RAATTY	4 cut(s)	365, 508, 578, 1977
XbaI	TCTAGA	1 cut(s)	1820
XceI	RCATGY	1 cut(s)	1546
XmiI	GTMKAC	2 cut(s)	708, 1341
XmnI	GAANNNNTTC	2 cut(s)	1146, 2079
XspI	CTAG	5 cut(s)	173, 269, 1314, 1821, 2039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.