AT5G03340

cell division

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013824
Physical Location & Seq
Reverse (-)
809830 .. 813293
3464 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03340.1

Sequence Viewer

Length: 2433 bp
ATGTCAAACGAACCGGAATCCTCCGACTCGAAAACTAAGAAGGATTTCAGTACTGCGATTCTAGAGAGGAAGAAGTCTCCAAACCGACTTGTTGTCGATGAAGCTATTAACGATGACAACTCTGTTGTCTCTCTCCATCCTACTACCATGGAAAAACTCCAGCTTTTCCGAGGCGATACAATTCTCATCAAGGGTAAGAAGAGGAAGGACACTGTATGCATTGCCCTCGCTGATGAAACATGTGAGGAACCCAAGATCAGAATGAATAAGGTTGTAAGGTCTAACTTGAGGGTTAGGCTGGGAGATGTTATTTCTGTTCACCAATGCCCAGATGTCAAGTATGGAAAGCGTGTTCACATCTTGCCTGTTGATGATACAGTTGAAGGCGTTACTGGAAACCTCTTTGATGCTTACCTGAAACCTTACTTTCTGGAGGCGTACCGTCCAGTGAGGAAGGGAGATCTTTTCCTAGTGAGAGGAGGAATGAGGAGTGTGGAGTTCAAAGTGATCGAGACTGATCCTGCTGAGTACTGTGTGGTGGCTCCAGATACAGAGATTTTCTGTGAGGGTGAACCAGTTAAGAGGGAGGACGAAGAAAGGCTTGATGAGGTTGGTTATGACGATGTTGGTGGTGTCAGGAAACAGATGGCTCAGATTAGGGAATTGGTTGAACTTCCCTTGAGGCATCCACAACTCTTTAAATCAATTGGTGTGAAGCCACCAAAGGGAATTCTGCTTTACGGACCTCCTGGATCCGGGAAGACTCTTATTGCTCGTGCTGTGGCTAATGAAACCGGAGCATTCTTCTTCTGTATCAATGGACCGGAAATCATGTCGAAACTAGCTGGTGAGAGTGAGAGCAACCTCAGGAAAGCATTTGAGGAGGCTGAGAAGAATGCCCCGTCTATCATATTCATTGATGAGATTGACTCTATTGCACCAAAAAGAGAGAAGACGAATGGTGAGGTTGAGAGGAGGATTGTCTCTCAGCTCCTTACGCTTATGGATGGACTAAAATCACGTGCCCATGTTATTGTTATGGGAGCAACAAATCGTCCCAACAGTATTGATCCGGCTTTGAGAAGGTTTGGAAGATTCGACAGGGAAATTGATATCGGTGTTCCTGATGAAATTGGACGTCTTGAAGTTCTTAGGATCCACACGAAGAACATGAAGCTAGCTGAAGATGTTGATCTAGAAAGAATCTCCAAGGACACGCATGGATATGTTGGAGCTGATCTTGCAGCTCTATGCACTGAAGCTGCCCTTCAATGTATCAGAGAGAAGATGGATGTGATTGACTTGGAAGATGACTCAATTGACGCCGAAATCCTCAATTCCATGGCTGTGTCTAATGAACACTTCCACACTGCTCTTGGAAACAGCAACCCATCCGCCCTTCGTGAAACTGTTGTGGAGGTTCCAAATGTCTCCTGGGAGGATATTGGAGGTCTCGAGAATGTTAAGAGAGAGCTTCAGGAGACTGTTCAATACCCTGTGGAGCATCCAGAGAAATTCGAGAAGTTTGGTATGTCTCCATCAAAGGGAGTTCTTTTCTACGGTCCTCCTGGATGTGGTAAAACCCTGTTAGCCAAAGCTATAGCAAACGAGTGCCAAGCAAACTTCATCAGTGTCAAGGGACCCGAGCTTCTCACAATGTGGTTTGGAGAAAGTGAAGCCAACGTCCGAGAAATCTTTGACAAGGCTCGTCAATCTGCTCCTTGTGTTCTGTTTTTCGATGAGCTAGACTCCATTGCTACTCAGAGAGGGAACAGCGCGGGTGATGCCGGCGGTGCAGCTGACAGAGTGTTGAATCAGCTTTTGACAGAAATGGATGGAATGAATGCAAAGAAGACGGTATTCATTATTGGAGCAACTAACAGACCGGATATCATAGATTCAGCTCTTCTTCGACCTGGAAGACTCGACCAGCTTATCTACATTCCTCTACCTGATGAAGATTCCCGTCTCAATATCTTTAAGGCCTGCTTGAGAAAATCTCCCGTCGCTAAAGATGTAGATGTTACTGCACTCGCAAAGTACACTCAGGGATTCAGTGGTGCTGATATCACCGAGATCTGCCAGAGAGCTTGCAAGTACGCCATCAGAGAAAACATCGAAAAGGATATTGAGAACGAGCGTAGAAGGAGCCAGAATCCAGAGGCCATGGAGGAAGATATGGTGGATGATGAAGTTTCAGAGATTAGAGCTGCTCATTTCGAAGAGTCGATGAAATATGCAAGGAGAAGTGTGAGTGATGCTGATATTCGCAAGTACCAAGCTTTTGCTCAGACGCTGCAGCAATCCAGAGGGTTTGGATCTGAGTTCAGGTTTGACTCAACCGCTGGTGTCGGACGCACCACTGGTGTTGCAGCCGCCGATCCCTTTGCCACTTCTGCAGCTGCAGCTGATGACGACGATCTCTACAGTTAG

Protein Analysis

810

Amino Acids

89.96

Weight (kDa)

5.08

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 29 - 110 9.8e-23 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 130 - 194 8.7e-12 Cell division protein 48 (CDC48), domain 2
AAA PF00004 244 - 373 1e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 396 - 437 8.2e-16 AAA+ lid domain
AAA PF00004 517 - 650 6.8e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 672 - 711 5.3e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1141
AccII CGCG 1 cut(s) 1778
AciI CCGC 5 cut(s) 1395, 1778, 1791, 2343, 2376
AclWI GGATC 8 cut(s) 512, 747, 760, 1064, 1150, 1163, 2326, 2375
AcsI RAATTY 2 cut(s) 729, 1514
AcuI CTGAAG 3 cut(s) 1203, 1278, 1460
AcvI CACGTG 1 cut(s) 1022
AcyI GRCGYC 2 cut(s) 1138, 1323
AdeI CACNNNGTG 1 cut(s) 1659
AfaI GTAC 6 cut(s) 52, 440, 530, 2042, 2099, 2276
AfiI CCNNNNNNNGG 4 cut(s) 725, 755, 1544, 1574
AflIII ACRYGT 1 cut(s) 239
AgsI TTSAA 7 cut(s) 383, 502, 671, 1145, 1271, 1490, 1813
AhdI GACNNNNNGTC 1 cut(s) 92
AjnI CCWGG 4 cut(s) 748, 1433, 1567, 1915
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
Alw26I GTCTC 9 cut(s) 81, 133, 506, 988, 1435, 1457, 1475, 1539, 1973
AlwI GGATC 8 cut(s) 512, 747, 760, 1064, 1150, 1163, 2326, 2375
AlwNI CAGNNNCTG 1 cut(s) 2296
Ama87I CYCGRG 2 cut(s) 1454, 1643
AoxI GGCC 2 cut(s) 1983, 2163
ApoI RAATTY 2 cut(s) 729, 1514
Asp700I GAANNNNTTC 1 cut(s) 44
AspLEI GCGC 1 cut(s) 1778
AspS9I GGNCC 4 cut(s) 743, 821, 1562, 1640
AsuC2I CCSGG 1 cut(s) 757
AsuHPI GGTGA 6 cut(s) 311, 581, 860, 974, 1793, 2062
AsuII TTCGAA 1 cut(s) 2220
AsuNHI GCTAGC 1 cut(s) 1177
AvaI CYCGRG 2 cut(s) 1454, 1643
AvaII GGWCC 4 cut(s) 743, 821, 1562, 1640
AxyI CCTNAGG 1 cut(s) 866
BaeGI GKGCMC 1 cut(s) 1027
BamHI GGATCC 2 cut(s) 752, 1155
BauI CACGAG 1 cut(s) 774
BbrPI CACGTG 1 cut(s) 1022
BbsI GAAGAC 4 cut(s) 767, 959, 1859, 1927
BccI CCATC 8 cut(s) 144, 640, 1001, 1282, 1399, 1546, 1829, 2111
BcgI CGANNNNNNTGC 4 cut(s) 208, 242, 2369, 2403
BciT130I CCWGG 4 cut(s) 750, 1435, 1569, 1917
BcnI CCSGG 1 cut(s) 757
BcoDI GTCTC 9 cut(s) 81, 133, 506, 988, 1435, 1457, 1475, 1539, 1973
BfaI CTAG 6 cut(s) 62, 470, 842, 1178, 1196, 1745
BfmI CTRYAG 5 cut(s) 1599, 2297, 2397, 2403, 2425
BglII AGATCT 2 cut(s) 460, 2076
BmcAI AGTACT 2 cut(s) 52, 530
Bme1390I CCNGG 5 cut(s) 750, 757, 1435, 1569, 1917
Bme18I GGWCC 4 cut(s) 743, 821, 1562, 1640
BmeRI GACNNNNNGTC 1 cut(s) 92
BmeT110I CYCGRG 2 cut(s) 1454, 1643
BmgT120I GGNCC 4 cut(s) 743, 821, 1562, 1640
BmiI GGNNCC 8 cut(s) 249, 543, 754, 1157, 1422, 1641, 1642, 2150
BmrFI CCNGG 5 cut(s) 750, 757, 1435, 1569, 1917
BmsI GCATC 5 cut(s) 397, 694, 1513, 1774, 2248
BmtI GCTAGC 1 cut(s) 1181
BpiI GAAGAC 4 cut(s) 767, 959, 1859, 1927
BplI GAGNNNNNCTC 6 cut(s) 914, 946, 1733, 1765, 1984, 2016
BpmI CTGGAG 3 cut(s) 143, 452, 528
Bpu14I TTCGAA 1 cut(s) 2220
BpuEI CTTGAG 3 cut(s) 307, 700, 2011
BpuMI CCSGG 1 cut(s) 757
BsaAI YACGTR 1 cut(s) 1022
BsaBI GATNNNNATC 1 cut(s) 1191
BsaHI GRCGYC 2 cut(s) 1138, 1323
BsaI GGTCTC 1 cut(s) 1457
BsaJI CCNNGG 6 cut(s) 147, 169, 1209, 1341, 1434, 2166
BsaWI WCCGGW 4 cut(s) 13, 794, 823, 1885
Bsc4I CCNNNNNNNGG 4 cut(s) 725, 755, 1544, 1574
Bse118I RCCGGY 1 cut(s) 1787
Bse1I ACTGG 4 cut(s) 397, 446, 575, 2368
Bse21I CCTNAGG 1 cut(s) 866
Bse3DI GCAATG 2 cut(s) 219, 1752
Bse8I GATNNNNATC 1 cut(s) 1191
BseBI CCWGG 4 cut(s) 750, 1435, 1569, 1917
BseDI CCNNGG 6 cut(s) 147, 169, 1209, 1341, 1434, 2166
BseGI GGATG 9 cut(s) 136, 685, 1012, 1297, 1391, 1504, 1577, 1840, 2191
BseJI GATNNNNATC 1 cut(s) 1191
BseLI CCNNNNNNNGG 4 cut(s) 725, 755, 1544, 1574
BseMI GCAATG 2 cut(s) 219, 1752
BseMII CTCAG 9 cut(s) 516, 665, 879, 880, 1001, 1775, 2060, 2303, 2313
BseNI ACTGG 4 cut(s) 397, 446, 575, 2368
BseRI GAGGAG 4 cut(s) 492, 502, 896, 988
BseSI GKGCMC 1 cut(s) 1027
BseYI CCCAGC 1 cut(s) 298
BsgI GTGCAG 2 cut(s) 1815, 2013
Bsh1236I CGCG 1 cut(s) 1778
BshFI GGCC 2 cut(s) 1985, 2165
BsiHKCI CYCGRG 2 cut(s) 1454, 1643
BsiSI CCGG 7 cut(s) 14, 756, 795, 824, 1073, 1788, 1886
BslFI GGGAC 2 cut(s) 1041, 1653
BslI CCNNNNNNNGG 4 cut(s) 725, 755, 1544, 1574
BsmAI GTCTC 9 cut(s) 81, 133, 506, 988, 1435, 1457, 1475, 1539, 1973
BsmBI CGTCTC 1 cut(s) 1973
BsmFI GGGAC 2 cut(s) 1041, 1653
BsmI GAATGC 3 cut(s) 800, 901, 1849
BsnI GGCC 2 cut(s) 1985, 2165
Bso31I GGTCTC 1 cut(s) 1457
BsoBI CYCGRG 2 cut(s) 1454, 1643
Bsp119I TTCGAA 1 cut(s) 2220
Bsp1286I GDGCHC 1 cut(s) 1027
Bsp19I CCATGG 3 cut(s) 147, 1341, 2166
BspACI CCGC 5 cut(s) 1395, 1778, 1791, 2343, 2376
BspANI GGCC 2 cut(s) 1985, 2165
BspCNI CTCAG 9 cut(s) 517, 664, 879, 880, 1000, 1774, 2059, 2302, 2314
BspFNI CGCG 1 cut(s) 1778
BspLI GGNNCC 8 cut(s) 249, 543, 754, 1157, 1422, 1641, 1642, 2150
BspMAI CTGCAG 3 cut(s) 2301, 2401, 2407
BspOI GCTAGC 1 cut(s) 1181
BspPI GGATC 8 cut(s) 512, 747, 760, 1064, 1150, 1163, 2326, 2375
BspQI GCTCTTC 1 cut(s) 1911
BspT104I TTCGAA 1 cut(s) 2220
BspTNI GGTCTC 1 cut(s) 1457
BsrDI GCAATG 2 cut(s) 219, 1752
BsrFI RCCGGY 1 cut(s) 1787
BsrI ACTGG 4 cut(s) 397, 446, 575, 2368
BssAI RCCGGY 1 cut(s) 1787
BssECI CCNNGG 6 cut(s) 147, 169, 1209, 1341, 1434, 2166
BssNI GRCGYC 2 cut(s) 1138, 1323
BssSI CACGAG 1 cut(s) 774
BssT1I CCWWGG 4 cut(s) 147, 1209, 1341, 2166
Bst2BI CACGAG 1 cut(s) 774
Bst2UI CCWGG 4 cut(s) 750, 1435, 1569, 1917
Bst6I CTCTTC 3 cut(s) 194, 1911, 2217
BstACI GRCGYC 2 cut(s) 1138, 1323
BstBAI YACGTR 1 cut(s) 1022
BstBI TTCGAA 1 cut(s) 2220
BstC8I GCNNGC 4 cut(s) 1179, 1789, 1987, 2092
BstDSI CCRYGG 3 cut(s) 147, 1341, 2166
BstF5I GGATG 9 cut(s) 136, 685, 1012, 1297, 1391, 1504, 1577, 1840, 2191
BstFNI CGCG 1 cut(s) 1778
BstHHI GCGC 1 cut(s) 1778
BstMAI GTCTC 9 cut(s) 81, 133, 506, 988, 1435, 1457, 1475, 1539, 1973
BstMWI GCNNNNNNNGC 6 cut(s) 997, 1241, 1784, 1793, 2396, 2405
BstNI CCWGG 4 cut(s) 750, 1435, 1569, 1917
BstNSI RCATGY 1 cut(s) 243
BstSCI CCNGG 5 cut(s) 748, 755, 1433, 1567, 1915
BstSFI CTRYAG 5 cut(s) 1599, 2297, 2397, 2403, 2425
BstSLI GKGCMC 1 cut(s) 1027
BstUI CGCG 1 cut(s) 1778
BstV2I GAAGAC 4 cut(s) 767, 959, 1859, 1927
BstX2I RGATCY 5 cut(s) 460, 752, 1155, 2076, 2318
BstYI RGATCY 5 cut(s) 460, 752, 1155, 2076, 2318
Bsu36I CCTNAGG 1 cut(s) 866
BsuRI GGCC 2 cut(s) 1985, 2165
BtgI CCRYGG 3 cut(s) 147, 1341, 2166
BtsCI GGATG 9 cut(s) 136, 685, 1012, 1297, 1391, 1504, 1577, 1840, 2191
BtsI GCAGTG 1 cut(s) 1368
BtsIMutI CAGTG 7 cut(s) 210, 453, 1254, 1368, 1636, 2062, 2361
Cac8I GCNNGC 4 cut(s) 1179, 1789, 1987, 2092
CaiI CAGNNNCTG 1 cut(s) 2296
CfoI GCGC 1 cut(s) 1778
Cfr10I RCCGGY 1 cut(s) 1787
Cfr13I GGNCC 4 cut(s) 743, 821, 1562, 1640
CseI GACGC 3 cut(s) 1331, 2302, 2364
Csp6I GTAC 6 cut(s) 51, 439, 529, 2041, 2098, 2275
CspCI CAANNNNNGTGG 2 cut(s) 2350, 2385
CviAII CATG 8 cut(s) 148, 240, 832, 1028, 1171, 1220, 1342, 2167
CviQI GTAC 6 cut(s) 51, 439, 529, 2041, 2098, 2275
DraI TTTAAA 1 cut(s) 700
DraIII CACNNNGTG 1 cut(s) 1659
DriI GACNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 3 cut(s) 194, 1911, 2217
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 3 cut(s) 194, 1911, 2217
EciI GGCGGA 1 cut(s) 1384
Eco130I CCWWGG 4 cut(s) 147, 1209, 1341, 2166
Eco147I AGGCCT 1 cut(s) 1985
Eco31I GGTCTC 1 cut(s) 1457
Eco32I GATATC 3 cut(s) 1114, 1891, 2068
Eco47I GGWCC 4 cut(s) 743, 821, 1562, 1640
Eco57I CTGAAG 3 cut(s) 1203, 1278, 1460
Eco72I CACGTG 1 cut(s) 1022
Eco81I CCTNAGG 1 cut(s) 866
Eco88I CYCGRG 2 cut(s) 1454, 1643
EcoO109I RGGNCCY 1 cut(s) 1640
EcoRI GAATTC 1 cut(s) 729
EcoRII CCWGG 4 cut(s) 748, 1433, 1567, 1915
EcoRV GATATC 3 cut(s) 1114, 1891, 2068
EcoT14I CCWWGG 4 cut(s) 147, 1209, 1341, 2166
EcoT22I ATGCAT 1 cut(s) 221
ErhI CCWWGG 4 cut(s) 147, 1209, 1341, 2166
Esp3I CGTCTC 1 cut(s) 1973
FaeI CATG 8 cut(s) 151, 243, 835, 1031, 1174, 1223, 1345, 2170
FalI AAGNNNNNCTT 6 cut(s) 585, 617, 1251, 1283, 1973, 2005
FaqI GGGAC 2 cut(s) 1041, 1653
FatI CATG 8 cut(s) 147, 239, 831, 1027, 1170, 1219, 1341, 2166
FauI CCCGC 1 cut(s) 1771
FokI GGATG 9 cut(s) 123, 672, 1019, 1304, 1378, 1491, 1584, 1847, 2198
FspBI CTAG 6 cut(s) 62, 470, 842, 1178, 1196, 1745
GlaI GCGC 1 cut(s) 1777
GsaI CCCAGC 1 cut(s) 302
GsuI CTGGAG 3 cut(s) 143, 452, 528
HaeIII GGCC 2 cut(s) 1985, 2165
HapII CCGG 7 cut(s) 14, 756, 795, 824, 1073, 1788, 1886
HgaI GACGC 3 cut(s) 1331, 2302, 2364
HhaI GCGC 1 cut(s) 1778
Hin1I GRCGYC 2 cut(s) 1138, 1323
Hin1II CATG 8 cut(s) 151, 243, 835, 1031, 1174, 1223, 1345, 2170
Hin6I GCGC 1 cut(s) 1776
HinP1I GCGC 1 cut(s) 1776
HindIII AAGCTT 1 cut(s) 2280
HpaII CCGG 7 cut(s) 14, 756, 795, 824, 1073, 1788, 1886
HphI GGTGA 6 cut(s) 311, 581, 860, 974, 1793, 2062
Hpy166II GTNNAC 4 cut(s) 319, 355, 572, 2043
Hpy8I GTNNAC 4 cut(s) 319, 355, 572, 2043
Hpy99I CGWCG 2 cut(s) 2009, 2420
HpyAV CCTTC 8 cut(s) 34, 199, 377, 448, 1077, 1277, 1409, 2139
HpyCH4IV ACGT 3 cut(s) 1021, 1138, 1683
HpyF10VI GCNNNNNNNGC 6 cut(s) 997, 1241, 1784, 1793, 2396, 2405
HpySE526I ACGT 3 cut(s) 1021, 1138, 1683
Hsp92I GRCGYC 2 cut(s) 1138, 1323
Hsp92II CATG 8 cut(s) 151, 243, 835, 1031, 1174, 1223, 1345, 2170
HspAI GCGC 1 cut(s) 1776
KflI GGGWCCC 1 cut(s) 1640
KroI GCCGGC 1 cut(s) 1787
KroNI GCCGGC 1 cut(s) 1789
LguI GCTCTTC 1 cut(s) 1911
LmnI GCTCC 9 cut(s) 547, 797, 996, 1043, 1232, 1501, 1723, 1871, 2148
LweI GCATC 5 cut(s) 397, 694, 1513, 1774, 2248
MaeI CTAG 6 cut(s) 62, 470, 842, 1178, 1196, 1745
MaeII ACGT 3 cut(s) 1021, 1138, 1683
MaeIII GTNAC 2 cut(s) 388, 2023
MfeI CAATTG 2 cut(s) 705, 1317
MflI RGATCY 5 cut(s) 460, 752, 1155, 2076, 2318
MhlI GDGCHC 1 cut(s) 1027
MluCI AATT 9 cut(s) 180, 662, 705, 729, 1107, 1131, 1317, 1336, 1514
MlyI GAGTC 8 cut(s) 20, 757, 923, 1307, 1742, 1917, 2234, 2330
MmeI TCCRAC 3 cut(s) 48, 1210, 2332
Mph1103I ATGCAT 1 cut(s) 221
MroNI GCCGGC 1 cut(s) 1787
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 5 cut(s) 108, 579, 699, 1464, 1980
MslI CAYNNNNRTG 2 cut(s) 1224, 1346
MspA1I CMGCKG 4 cut(s) 1799, 2345, 2402, 2408
MspI CCGG 7 cut(s) 14, 756, 795, 824, 1073, 1788, 1886
MspR9I CCNGG 5 cut(s) 750, 757, 1435, 1569, 1917
MunI CAATTG 2 cut(s) 705, 1317
Mva1269I GAATGC 3 cut(s) 800, 901, 1849
MvaI CCWGG 4 cut(s) 750, 1435, 1569, 1917
MvnI CGCG 1 cut(s) 1778
MwoI GCNNNNNNNGC 6 cut(s) 997, 1241, 1784, 1793, 2396, 2405
NaeI GCCGGC 1 cut(s) 1789
NciI CCSGG 1 cut(s) 757
NcoI CCATGG 3 cut(s) 147, 1341, 2166
NgoMIV GCCGGC 1 cut(s) 1787
NheI GCTAGC 1 cut(s) 1177
NlaIII CATG 8 cut(s) 151, 243, 835, 1031, 1174, 1223, 1345, 2170
NlaIV GGNNCC 8 cut(s) 249, 543, 754, 1157, 1422, 1641, 1642, 2150
NsiI ATGCAT 1 cut(s) 221
NspI RCATGY 1 cut(s) 243
NspV TTCGAA 1 cut(s) 2220
PaeR7I CTCGAG 1 cut(s) 1454
PceI AGGCCT 1 cut(s) 1985
PciI ACATGT 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 1911
PctI GAATGC 3 cut(s) 800, 901, 1849
PdiI GCCGGC 1 cut(s) 1789
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 9 cut(s) 17, 58, 1095, 1203, 1813, 1898, 1961, 2052, 2155
PfoI TCCNGGA 3 cut(s) 748, 755, 1567
PleI GAGTC 8 cut(s) 20, 757, 923, 1307, 1742, 1917, 2233, 2330
PmaCI CACGTG 1 cut(s) 1022
PmlI CACGTG 1 cut(s) 1022
PpsI GAGTC 8 cut(s) 20, 757, 923, 1307, 1742, 1917, 2233, 2330
Ppu21I YACGTR 1 cut(s) 1022
PpuMI RGGWCCY 1 cut(s) 1640
PscI ACATGT 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 1640
Psp6I CCWGG 4 cut(s) 748, 1433, 1567, 1915
PspCI CACGTG 1 cut(s) 1022
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 4 cut(s) 748, 1433, 1567, 1915
PspN4I GGNNCC 8 cut(s) 249, 543, 754, 1157, 1422, 1641, 1642, 2150
PspPI GGNCC 4 cut(s) 743, 821, 1562, 1640
PspPPI RGGWCCY 1 cut(s) 1640
PstI CTGCAG 3 cut(s) 2301, 2401, 2407
PstNI CAGNNNCTG 1 cut(s) 2296
PsuI RGATCY 5 cut(s) 460, 752, 1155, 2076, 2318
PvuII CAGCTG 3 cut(s) 1799, 2402, 2408
RsaI GTAC 6 cut(s) 52, 440, 530, 2042, 2099, 2276
RsaNI GTAC 6 cut(s) 51, 439, 529, 2041, 2098, 2275
RseI CAYNNNNRTG 2 cut(s) 1224, 1346
SapI GCTCTTC 1 cut(s) 1911
SaqAI TTAA 5 cut(s) 108, 579, 699, 1464, 1980
Sau96I GGNCC 4 cut(s) 743, 821, 1562, 1640
ScaI AGTACT 2 cut(s) 52, 530
SchI GAGTC 8 cut(s) 20, 757, 923, 1307, 1742, 1917, 2234, 2330
ScrFI CCNGG 5 cut(s) 750, 757, 1435, 1569, 1917
SduI GDGCHC 1 cut(s) 1027
SfaNI GCATC 5 cut(s) 397, 694, 1513, 1774, 2248
SfcI CTRYAG 5 cut(s) 1599, 2297, 2397, 2403, 2425
Sfr274I CTCGAG 1 cut(s) 1454
SfuI TTCGAA 1 cut(s) 2220
SinI GGWCC 4 cut(s) 743, 821, 1562, 1640
SlaI CTCGAG 1 cut(s) 1454
SmiMI CAYNNNNRTG 2 cut(s) 1224, 1346
SmlI CTYRAG 4 cut(s) 286, 679, 1454, 1990
SmoI CTYRAG 4 cut(s) 286, 679, 1454, 1990
Sse9I AATT 9 cut(s) 180, 662, 705, 729, 1107, 1131, 1317, 1336, 1514
SseBI AGGCCT 1 cut(s) 1985
SsiI CCGC 5 cut(s) 1395, 1778, 1791, 2343, 2376
SspMI CTAG 6 cut(s) 62, 470, 842, 1178, 1196, 1745
StuI AGGCCT 1 cut(s) 1985
StyD4I CCNGG 5 cut(s) 748, 755, 1433, 1567, 1915
StyI CCWWGG 4 cut(s) 147, 1209, 1341, 2166
TaiI ACGT 3 cut(s) 1024, 1141, 1686
TasI AATT 9 cut(s) 180, 662, 705, 729, 1107, 1131, 1317, 1336, 1514
TatI WGTACW 3 cut(s) 50, 528, 2040
TauI GCSGC 1 cut(s) 2378
TfiI GAWTC 9 cut(s) 17, 58, 1095, 1203, 1813, 1898, 1961, 2052, 2155
Tru1I TTAA 5 cut(s) 108, 579, 699, 1464, 1980
Tru9I TTAA 5 cut(s) 108, 579, 699, 1464, 1980
TscAI CASTG 7 cut(s) 217, 453, 1261, 1375, 1636, 2062, 2368
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 7 cut(s) 217, 453, 1261, 1375, 1636, 2062, 2368
VpaK11BI GGWCC 4 cut(s) 743, 821, 1562, 1640
XapI RAATTY 2 cut(s) 729, 1514
XbaI TCTAGA 2 cut(s) 61, 1195
XceI RCATGY 1 cut(s) 243
XcmI CCANNNNNNNNNTGG 2 cut(s) 1373, 1431
XhoI CTCGAG 1 cut(s) 1454
XmnI GAANNNNTTC 1 cut(s) 44
XspI CTAG 6 cut(s) 62, 470, 842, 1178, 1196, 1745
ZraI GACGTC 1 cut(s) 1139
ZrmI AGTACT 2 cut(s) 52, 530
Zsp2I ATGCAT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.