Rroxscaffold_3G00262420

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013824
Physical Location & Seq
Forward (+)
56423300 .. 56427545
4246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262420.1

Sequence Viewer

Length: 2430 bp
ATGGCGGATCCGAGCTCATCTGCCCCAATTCCTGACCCGAAAACTAAGAAGGATTTTTCCACTGCAATTTTGGAGCGAAAGAAGTCTCCTAATCGACTTGTGGTTGATGAAGCTATCAATGATGATAATTCTGTGGTGTCCTTGCACCCTGCTACCATGGAAAAACTTCAGTTTTTTCGGGGTGACACTGTGCTAATAAAGGGGAAGAAACGAAAAGACACAGTTTGCATTGTTCTTGCTGATGAACAATGTGAAGAACCTAAAATCAGAATGAACAAAGTTGTAAGGGCTAATCTTAGGGTTCGTCTTGGAGATGTTGTGTCGGTTCATACTTGCCCTGATGTTAAATATGGGAAGCGGGTTCACATCCTTCCAATCGATGATACAATTGAAGGCGTAACTGGAAACTTGTTTGATGCATATTTGAAGCCATACTTTTTGGAATCTTACCGGCCTGTGAGGAAGGGTGACCTTTTCCTGGTCAGGGGTGGGATGCGAAGTGTTGAGTTCAAGGTCATTGAGACAGACCCTGGTGAATACTGTGTTGTTGCTCCCGATACTGAGATTTTCTGTGAGGGAGAACCTGTGAAACGTGAGGATGAGGAAAAGTTGAATGAGGTGGGCTATGATGATGTTGGAGGTGTTAGGAAGCAAATGGCTCAGATACGCGAGTTAGTAGAGTTACCTCTTAGACACCCACAGCTCTTCAAGTCCATTGGAGTGAAACCACCTAAAGGGATTCTGCTTTATGGACCACCTGGGTCTGGGAAAACTTTGATTGCAAGGGCTGTAGCTAATGAGACTGGAGCATTCTTCTTTTTGATTAATGGGCCTGAAATAATGTCAAAGTTAGCTGGTGAGAGTGAAAGCAACTTGAGGAAGGCATTTGAAGAAGCTGAAAAGAATGCCCCATCTATCATTTTTATTGATGAGTTAGATTCCATTGCTCCAAAGAGGGAGAAGACTCAGGGTGAAGTGGAAAGGCGTATAGTATCGCAACTTCTGACTTTGATGGATGGTCTCAAGTCCCGTGCACATGTTATTGTCATGGGAGCTACAAATAGGCCAAACAGCATTGACCCTGCTCTGAGGAGGTTTGGAAGATTTGATAGAGAGATTGACATAGGTGTACCGGATGAAGTTGGAAGATTGGAAGTTTTGAGAATCCATACAAAGAACATGAAACTTGCAGAAGATGTTGATCTCGAACGAGTTTCAAAGGATACCCATGGTTATGTCGGTGCTGATCTTGCCGCTCTCTGTACTGAGGCTGCTCTTCAGTGCATCCGTGAGAAAATGGATGTTATTGACTTGGAAGATGAGGAAATTGATGCTGAGGTATTGAATTCTATGGCTGTGACGAATGAACACTTCCAGACTGCATTGGGTGCTTCAAATCCTTCAGCATTGCGTGAAACTGTTGTGGAGGTACCTAATGTCTCATGGGACGCTATTGGTGGATTGGACAATGTCAAAAGAGAACTTCAAGAGACTGTTCAATACCCAGTGGAGCATCCTGAGAAGTTTGAGAAATTTGGCATGTCACCTTCTAAAGGTGTTCTCTTTTATGGGCCACCTGGCTGTGGTAAAACCCTTCTTGCTAAGGCAATTGCCAATGAATGCCAGGCGAATTTCATTAGCATCAAAGGCCCCGAGTTACTGACAATGTGGTTTGGAGAAAGTGAGGCAAACGTCCGTGAGATATTTGACAAGGCTCGCCAATCTGCTCCTTGTGTTCTTTTCTTTGATGAGCTGGACTCGATTGCAACTCAGCGTGGAAATTCTTCTGGAGATGCTGGTGGTGCTGCAGATAGAGTCTTGAACCAACTTCTAACTGAAATGGATGGAATGAGTGCAAAGAAAACAGTTTTTATCATTGGAGCAACAAACAGGCCAGACATTATTGACCCAGCATTGCTCAGGCCTGGACGTCTTGACCAGCTCATCTATATCCCACTCCCTGATGAAGCTTCCCGTCTTCAGATTTTCAAAGCATGTTTGCGGAAGTCACCCGTCTCTAAGGATGTTGACCTTGCAGTTCTTGCGCATCATACCCATGGTTTTAGTGGTGCTGATATCACTGAGATATGTCAGCGTGCCTGCAAATATGCCATCAGAGAAAATATTGAGAAGGATATTGAGAAGGAGAGAAGAAGGGCAGAGAACCCAGAAGCGATGGAGGAGGATGACATTGATGAGGTTCCCGAGATAAAGGCCGCACACTTTGAGGAATCCATGAAATTTGCCCGCAGAAGTGTCAGTGATGCTGACATCAGGAAGTACCAGGCATTCGCTCAGACTCTGCAGCAATCTCGTGGGCTTGGATCTGACTTCCGGTTCCCCCAAACGGGCACCACTGCTGGAGGAGCAGCTCCAGACCCTTTTGCTACGGCCGCAGCTGCTGCAGATGACGATGATCTGTACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

809

Amino Acids

89.56

Weight (kDa)

5.17

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 32 - 113 1.2e-23 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 133 - 197 1.2e-11 Cell division protein 48 (CDC48), domain 2
AAA PF00004 247 - 376 1.4e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 399 - 442 1.2e-15 AAA+ lid domain
AAA PF00004 520 - 653 1.1e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 675 - 715 4.3e-13 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1933
Acc16I TGCGCA 1 cut(s) 2046
Acc65I GGTACC 1 cut(s) 1429
AccB1I GGYRCC 2 cut(s) 1429, 2351
AccBSI CCGCTC 1 cut(s) 1256
AccII CGCG 1 cut(s) 669
AciI CCGC 7 cut(s) 5, 358, 1254, 2002, 2217, 2248, 2394
AclWI GGATC 3 cut(s) 2, 15, 2332
AcoI YGGCCR 1 cut(s) 2391
AcsI RAATTY 5 cut(s) 1345, 1532, 1630, 1780, 2240
AcuI CTGAAG 4 cut(s) 152, 1262, 1386, 1964
AcyI GRCGYC 1 cut(s) 1930
AfaI GTAC 5 cut(s) 1131, 1264, 1431, 2282, 2423
AfiI CCNNNNNNNGG 8 cut(s) 478, 484, 734, 764, 1553, 1583, 2347, 2348
AflIII ACRYGT 1 cut(s) 1036
AjnI CCWGG 7 cut(s) 477, 529, 757, 1576, 1623, 1924, 2283
Alw21I GWGCWC 2 cut(s) 17, 1036
Alw26I GTCTC 7 cut(s) 90, 515, 794, 1025, 1444, 1484, 2020
Alw44I GTGCAC 1 cut(s) 1032
AlwI GGATC 3 cut(s) 2, 15, 2332
AlwNI CAGNNNCTG 3 cut(s) 530, 2302, 2402
Ama87I CYCGRG 2 cut(s) 1652, 2204
AoxI GGCC 9 cut(s) 452, 830, 1064, 1571, 1648, 1892, 1922, 2214, 2391
ApaLI GTGCAC 1 cut(s) 1032
ApeKI GCWGC 7 cut(s) 1271, 1805, 2305, 2369, 2396, 2399, 2402
ApoI RAATTY 5 cut(s) 1345, 1532, 1630, 1780, 2240
AseI ATTAAT 1 cut(s) 825
Asp700I GAANNNNTTC 2 cut(s) 165, 1783
Asp718I GGTACC 1 cut(s) 1429
AspLEI GCGC 1 cut(s) 2047
AspS9I GGNCC 4 cut(s) 752, 830, 1571, 1649
AsuHPI GGTGA 7 cut(s) 194, 479, 545, 869, 983, 1536, 2001
AvaI CYCGRG 2 cut(s) 1652, 2204
AvaII GGWCC 1 cut(s) 752
BaeGI GKGCMC 2 cut(s) 1036, 2354
BaeI ACNNNNGTAYC 2 cut(s) 656, 689
BamHI GGATCC 1 cut(s) 7
BanI GGYRCC 2 cut(s) 1429, 2351
BanII GRGCYC 1 cut(s) 17
BarI GAAGNNNNNNTAC 2 cut(s) 984, 1016
BauI CACGAG 1 cut(s) 2313
BbsI GAAGAC 2 cut(s) 968, 1970
Bbv12I GWGCWC 2 cut(s) 17, 1036
BbvCI CCTCAGC 1 cut(s) 1335
BbvI GCAGC 7 cut(s) 1258, 1792, 2317, 2381, 2386, 2389, 2408
BccI CCATC 6 cut(s) 919, 1006, 1010, 1838, 2120, 2170
BceAI ACGGC 1 cut(s) 2406
BcgI CGANNNNNNTGC 2 cut(s) 2294, 2328
BciT130I CCWGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
BciVI GTATCC 1 cut(s) 1216
BcoDI GTCTC 7 cut(s) 90, 515, 794, 1025, 1444, 1484, 2020
BfmI CTRYAG 4 cut(s) 789, 1806, 2303, 2403
BfuI GTATCC 1 cut(s) 1216
Bme1390I CCNGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
Bme18I GGWCC 1 cut(s) 752
BmeT110I CYCGRG 2 cut(s) 1652, 2204
BmgT120I GGNCC 4 cut(s) 752, 830, 1571, 1649
BmiI GGNNCC 6 cut(s) 9, 1431, 1651, 2202, 2339, 2353
BmrFI CCNGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
BmrI ACTGGG 1 cut(s) 1499
BmsI GCATC 9 cut(s) 406, 483, 1293, 1321, 1522, 1650, 1783, 2056, 2254
BmuI ACTGGG 1 cut(s) 1499
BpiI GAAGAC 2 cut(s) 968, 1970
BplI GAGNNNNNCTC 2 cut(s) 1742, 1774
BpmI CTGGAG 4 cut(s) 825, 1809, 2358, 2382
Bpu10I CCTNAGC 3 cut(s) 1335, 1602, 1919
BpuEI CTTGAG 2 cut(s) 895, 1007
Bsa29I ATCGAT 1 cut(s) 378
BsaBI GATNNNNATC 1 cut(s) 1200
BsaHI GRCGYC 1 cut(s) 1930
BsaI GGTCTC 1 cut(s) 1025
BsaJI CCNNGG 5 cut(s) 156, 529, 758, 1228, 2056
BsaWI WCCGGW 2 cut(s) 1132, 2334
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 570, 600
Bsc4I CCNNNNNNNGG 8 cut(s) 478, 484, 734, 764, 1553, 1583, 2347, 2348
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 3 cut(s) 406, 808, 1505
Bse3DI GCAATG 3 cut(s) 942, 1406, 1913
Bse8I GATNNNNATC 1 cut(s) 1200
BseBI CCWGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 5 cut(s) 156, 529, 758, 1228, 2056
BseJI GATNNNNATC 1 cut(s) 1200
BseLI CCNNNNNNNGG 8 cut(s) 478, 484, 734, 764, 1553, 1583, 2347, 2348
BseMI GCAATG 3 cut(s) 942, 1406, 1913
BseNI ACTGG 3 cut(s) 406, 808, 1505
BseRI GAGGAG 3 cut(s) 1105, 2195, 2379
BseSI GKGCMC 2 cut(s) 1036, 2354
BseX3I CGGCCG 1 cut(s) 2391
BseXI GCAGC 7 cut(s) 1258, 1792, 2317, 2381, 2386, 2389, 2408
BseYI CCCAGC 1 cut(s) 1909
Bsh1236I CGCG 1 cut(s) 669
Bsh1285I CGRYCG 1 cut(s) 2394
BshFI GGCC 9 cut(s) 454, 832, 1066, 1573, 1650, 1894, 1924, 2216, 2393
BshNI GGYRCC 2 cut(s) 1429, 2351
BshVI ATCGAT 1 cut(s) 378
BsiEI CGRYCG 1 cut(s) 2394
BsiHKAI GWGCWC 2 cut(s) 17, 1036
BsiHKCI CYCGRG 2 cut(s) 1652, 2204
BsiSI CCGG 3 cut(s) 451, 1133, 2335
BslFI GGGAC 2 cut(s) 1012, 1460
BslI CCNNNNNNNGG 8 cut(s) 478, 484, 734, 764, 1553, 1583, 2347, 2348
BsmAI GTCTC 7 cut(s) 90, 515, 794, 1025, 1444, 1484, 2020
BsmBI CGTCTC 1 cut(s) 2020
BsmFI GGGAC 2 cut(s) 1012, 1460
BsmI GAATGC 4 cut(s) 809, 910, 1625, 2288
BsnI GGCC 9 cut(s) 454, 832, 1066, 1573, 1650, 1894, 1924, 2216, 2393
Bso31I GGTCTC 1 cut(s) 1025
BsoBI CYCGRG 2 cut(s) 1652, 2204
Bsp1286I GDGCHC 3 cut(s) 17, 1036, 2354
Bsp1407I TGTACA 1 cut(s) 2421
Bsp143I GATC 5 cut(s) 7, 1201, 1246, 2324, 2416
Bsp19I CCATGG 3 cut(s) 156, 1228, 2056
BspACI CCGC 7 cut(s) 5, 358, 1254, 2002, 2217, 2248, 2394
BspANI GGCC 9 cut(s) 454, 832, 1066, 1573, 1650, 1894, 1924, 2216, 2393
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 1 cut(s) 669
BspLI GGNNCC 6 cut(s) 9, 1431, 1651, 2202, 2339, 2353
BspMAI CTGCAG 3 cut(s) 1810, 2307, 2407
BspPI GGATC 3 cut(s) 2, 15, 2332
BspQI GCTCTTC 2 cut(s) 710, 1281
BspT107I GGYRCC 2 cut(s) 1429, 2351
BspTNI GGTCTC 1 cut(s) 1025
BsrBI CCGCTC 1 cut(s) 1256
BsrDI GCAATG 3 cut(s) 942, 1406, 1913
BsrFI RCCGGY 1 cut(s) 450
BsrGI TGTACA 1 cut(s) 2421
BsrI ACTGG 3 cut(s) 406, 808, 1505
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 5 cut(s) 156, 529, 758, 1228, 2056
BssMI GATC 5 cut(s) 7, 1201, 1246, 2324, 2416
BssNI GRCGYC 1 cut(s) 1930
BssSI CACGAG 1 cut(s) 2313
BssT1I CCWWGG 3 cut(s) 156, 1228, 2056
Bst2BI CACGAG 1 cut(s) 2313
Bst2UI CCWGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
Bst4CI ACNGT 6 cut(s) 190, 223, 542, 1420, 1495, 1867
Bst6I CTCTTC 2 cut(s) 710, 1281
BstACI GRCGYC 1 cut(s) 1930
BstAPI GCANNNNNTGC 3 cut(s) 1388, 2042, 2402
BstAUI TGTACA 1 cut(s) 2421
BstC8I GCNNGC 4 cut(s) 1717, 2097, 2101, 2248
BstDSI CCRYGG 3 cut(s) 156, 1228, 2056
BstEII GGTNACC 1 cut(s) 467
BstENI CCTNNNNNAGG 1 cut(s) 1551
BstFNI CGCG 1 cut(s) 669
BstHHI GCGC 1 cut(s) 2047
BstKTI GATC 5 cut(s) 10, 1204, 1249, 2327, 2419
BstMAI GTCTC 7 cut(s) 90, 515, 794, 1025, 1444, 1484, 2020
BstMBI GATC 5 cut(s) 7, 1201, 1246, 2324, 2416
BstMCI CGRYCG 1 cut(s) 2394
BstMWI GCNNNNNNNGC 9 cut(s) 1250, 1388, 1647, 1802, 2042, 2366, 2393, 2399, 2402
BstNI CCWGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
BstNSI RCATGY 3 cut(s) 1040, 1543, 1998
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 7 cut(s) 477, 529, 757, 1576, 1623, 1924, 2283
BstSFI CTRYAG 4 cut(s) 789, 1806, 2303, 2403
BstSLI GKGCMC 2 cut(s) 1036, 2354
BstUI CGCG 1 cut(s) 669
BstV1I GCAGC 7 cut(s) 1258, 1792, 2317, 2381, 2386, 2389, 2408
BstV2I GAAGAC 2 cut(s) 968, 1970
BstX2I RGATCY 2 cut(s) 7, 2324
BstYI RGATCY 2 cut(s) 7, 2324
BstZI CGGCCG 1 cut(s) 2391
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 1216
BsuRI GGCC 9 cut(s) 454, 832, 1066, 1573, 1650, 1894, 1924, 2216, 2393
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 3 cut(s) 156, 1228, 2056
BtgZI GCGATG 1 cut(s) 2189
BtsI GCAGTG 2 cut(s) 60, 2355
BtsIMutI CAGTG 7 cut(s) 60, 186, 1286, 1512, 2079, 2266, 2355
Cac8I GCNNGC 4 cut(s) 1717, 2097, 2101, 2248
CaiI CAGNNNCTG 3 cut(s) 530, 2302, 2402
CfoI GCGC 1 cut(s) 2047
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 4 cut(s) 752, 830, 1571, 1649
ClaI ATCGAT 1 cut(s) 378
CseI GACGC 1 cut(s) 1457
Csp6I GTAC 5 cut(s) 1130, 1263, 1430, 2281, 2422
CviQI GTAC 5 cut(s) 1130, 1263, 1430, 2281, 2422
DpnI GATC 5 cut(s) 9, 1203, 1248, 2326, 2418
DpnII GATC 5 cut(s) 7, 1201, 1246, 2324, 2416
EaeI YGGCCR 1 cut(s) 2391
EagI CGGCCG 1 cut(s) 2391
Eam1104I CTCTTC 2 cut(s) 710, 1281
EarI CTCTTC 2 cut(s) 710, 1281
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 15
EclXI CGGCCG 1 cut(s) 2391
Eco130I CCWWGG 3 cut(s) 156, 1228, 2056
Eco147I AGGCCT 1 cut(s) 1924
Eco24I GRGCYC 1 cut(s) 17
Eco31I GGTCTC 1 cut(s) 1025
Eco32I GATATC 1 cut(s) 2077
Eco47I GGWCC 1 cut(s) 752
Eco52I CGGCCG 1 cut(s) 2391
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 4 cut(s) 152, 1262, 1386, 1964
Eco88I CYCGRG 2 cut(s) 1652, 2204
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 15
EcoNI CCTNNNNNAGG 1 cut(s) 1551
EcoO109I RGGNCCY 1 cut(s) 1649
EcoO65I GGTNACC 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 1345
EcoRII CCWGG 7 cut(s) 477, 529, 757, 1576, 1623, 1924, 2283
EcoRV GATATC 1 cut(s) 2077
EcoT14I CCWWGG 3 cut(s) 156, 1228, 2056
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 3 cut(s) 156, 1228, 2056
Esp3I CGTCTC 1 cut(s) 2020
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 2 cut(s) 1012, 1460
FauI CCCGC 2 cut(s) 351, 2255
FriOI GRGCYC 1 cut(s) 17
FspI TGCGCA 1 cut(s) 2046
GlaI GCGC 1 cut(s) 2046
GsaI CCCAGC 1 cut(s) 1913
GsuI CTGGAG 4 cut(s) 825, 1809, 2358, 2382
HaeIII GGCC 9 cut(s) 454, 832, 1066, 1573, 1650, 1894, 1924, 2216, 2393
HapII CCGG 3 cut(s) 451, 1133, 2335
HgaI GACGC 1 cut(s) 1457
HhaI GCGC 1 cut(s) 2047
Hin1I GRCGYC 1 cut(s) 1930
Hin6I GCGC 1 cut(s) 2045
HinP1I GCGC 1 cut(s) 2045
HincII GTYRAC 1 cut(s) 2029
HindII GTYRAC 1 cut(s) 2029
HindIII AAGCTT 1 cut(s) 1968
HinfI GANTC 9 cut(s) 443, 739, 938, 964, 1164, 1757, 1815, 2231, 2299
HpaII CCGG 3 cut(s) 451, 1133, 2335
HphI GGTGA 7 cut(s) 194, 479, 545, 869, 983, 1536, 2001
Hpy166II GTNNAC 4 cut(s) 364, 1034, 1130, 2029
Hpy188I TCNGA 9 cut(s) 12, 269, 663, 1005, 1089, 1983, 2117, 2298, 2329
Hpy8I GTNNAC 4 cut(s) 364, 1034, 1130, 2029
HpyCH4III ACNGT 6 cut(s) 190, 223, 542, 1420, 1495, 1867
HpyCH4IV ACGT 3 cut(s) 592, 1692, 1930
HpyF10VI GCNNNNNNNGC 9 cut(s) 1250, 1388, 1647, 1802, 2042, 2366, 2393, 2399, 2402
HpySE526I ACGT 3 cut(s) 592, 1692, 1930
Hsp92I GRCGYC 1 cut(s) 1930
HspAI GCGC 1 cut(s) 2045
KpnI GGTACC 1 cut(s) 1433
Kzo9I GATC 5 cut(s) 7, 1201, 1246, 2324, 2416
LguI GCTCTTC 2 cut(s) 710, 1281
Lsp1109I GCAGC 7 cut(s) 1258, 1792, 2317, 2381, 2386, 2389, 2408
LweI GCATC 9 cut(s) 406, 483, 1293, 1321, 1522, 1650, 1783, 2056, 2254
MaeII ACGT 3 cut(s) 592, 1692, 1930
MaeIII GTNAC 8 cut(s) 182, 397, 467, 681, 1357, 1542, 1656, 2007
MalI GATC 5 cut(s) 9, 1203, 1248, 2326, 2418
MbiI CCGCTC 1 cut(s) 1256
MboI GATC 5 cut(s) 7, 1201, 1246, 2324, 2416
MfeI CAATTG 2 cut(s) 387, 1608
MflI RGATCY 2 cut(s) 7, 2324
MhlI GDGCHC 3 cut(s) 17, 1036, 2354
MlyI GAGTC 4 cut(s) 958, 1751, 1824, 2293
MmeI TCCRAC 2 cut(s) 616, 1123
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 2 cut(s) 165, 1783
MseI TTAA 2 cut(s) 345, 825
MslI CAYNNNNRTG 3 cut(s) 719, 1233, 2055
MspA1I CMGCKG 2 cut(s) 2399, 2427
MspI CCGG 3 cut(s) 451, 1133, 2335
MspR9I CCNGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
MunI CAATTG 2 cut(s) 387, 1608
Mva1269I GAATGC 4 cut(s) 809, 910, 1625, 2288
MvaI CCWGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
MvnI CGCG 1 cut(s) 669
MwoI GCNNNNNNNGC 9 cut(s) 1250, 1388, 1647, 1802, 2042, 2366, 2393, 2399, 2402
NcoI CCATGG 3 cut(s) 156, 1228, 2056
NdeII GATC 5 cut(s) 7, 1201, 1246, 2324, 2416
NlaIV GGNNCC 6 cut(s) 9, 1431, 1651, 2202, 2339, 2353
NmuCI GTSAC 5 cut(s) 182, 467, 1357, 1542, 2007
NsbI TGCGCA 1 cut(s) 2046
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 3 cut(s) 1040, 1543, 1998
PceI AGGCCT 1 cut(s) 1924
PciI ACATGT 1 cut(s) 1036
PciSI GCTCTTC 2 cut(s) 710, 1281
PctI GAATGC 4 cut(s) 809, 910, 1625, 2288
PdmI GAANNNNTTC 2 cut(s) 165, 1783
PfeI GAWTC 5 cut(s) 443, 739, 938, 1164, 2231
PflFI GACNNNGTC 1 cut(s) 1469
PleI GAGTC 4 cut(s) 958, 1751, 1823, 2293
PpsI GAGTC 4 cut(s) 958, 1751, 1823, 2293
PscI ACATGT 1 cut(s) 1036
PshBI ATTAAT 1 cut(s) 825
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 7 cut(s) 477, 529, 757, 1576, 1623, 1924, 2283
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 1909
PspGI CCWGG 7 cut(s) 477, 529, 757, 1576, 1623, 1924, 2283
PspN4I GGNNCC 6 cut(s) 9, 1431, 1651, 2202, 2339, 2353
PspPI GGNCC 4 cut(s) 752, 830, 1571, 1649
PstI CTGCAG 3 cut(s) 1810, 2307, 2407
PstNI CAGNNNCTG 3 cut(s) 530, 2302, 2402
PsuI RGATCY 2 cut(s) 7, 2324
PsyI GACNNNGTC 1 cut(s) 1469
PvuII CAGCTG 2 cut(s) 2399, 2427
RsaI GTAC 5 cut(s) 1131, 1264, 1431, 2282, 2423
RsaNI GTAC 5 cut(s) 1130, 1263, 1430, 2281, 2422
RseI CAYNNNNRTG 3 cut(s) 719, 1233, 2055
SacI GAGCTC 1 cut(s) 17
SapI GCTCTTC 2 cut(s) 710, 1281
SaqAI TTAA 2 cut(s) 345, 825
Sau3AI GATC 5 cut(s) 7, 1201, 1246, 2324, 2416
Sau96I GGNCC 4 cut(s) 752, 830, 1571, 1649
SchI GAGTC 4 cut(s) 958, 1751, 1824, 2293
ScrFI CCNGG 7 cut(s) 479, 531, 759, 1578, 1625, 1926, 2285
SduI GDGCHC 3 cut(s) 17, 1036, 2354
SfaNI GCATC 9 cut(s) 406, 483, 1293, 1321, 1522, 1650, 1783, 2056, 2254
SfcI CTRYAG 4 cut(s) 789, 1806, 2303, 2403
SinI GGWCC 1 cut(s) 752
SmiMI CAYNNNNRTG 3 cut(s) 719, 1233, 2055
SmlI CTYRAG 2 cut(s) 874, 1022
SmoI CTYRAG 2 cut(s) 874, 1022
SseBI AGGCCT 1 cut(s) 1924
SsiI CCGC 7 cut(s) 5, 358, 1254, 2002, 2217, 2248, 2394
SspI AATATT 1 cut(s) 2125
SstI GAGCTC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 1924
StyD4I CCNGG 7 cut(s) 477, 529, 757, 1576, 1623, 1924, 2283
StyI CCWWGG 3 cut(s) 156, 1228, 2056
TaaI ACNGT 6 cut(s) 190, 223, 542, 1420, 1495, 1867
TaiI ACGT 3 cut(s) 595, 1695, 1933
TaqI TCGA 4 cut(s) 94, 378, 1206, 1760
TatI WGTACW 2 cut(s) 1262, 2421
TauI GCSGC 3 cut(s) 1256, 2219, 2396
TfiI GAWTC 5 cut(s) 443, 739, 938, 1164, 2231
Tru1I TTAA 2 cut(s) 345, 825
Tru9I TTAA 2 cut(s) 345, 825
TscAI CASTG 7 cut(s) 67, 193, 1286, 1512, 2086, 2266, 2362
TseFI GTSAC 5 cut(s) 182, 467, 1357, 1542, 2007
TseI GCWGC 7 cut(s) 1271, 1805, 2305, 2369, 2396, 2399, 2402
Tsp45I GTSAC 5 cut(s) 182, 467, 1357, 1542, 2007
TspGWI ACGGA 2 cut(s) 1277, 1685
TspRI CASTG 7 cut(s) 67, 193, 1286, 1512, 2086, 2266, 2362
Tth111I GACNNNGTC 1 cut(s) 1469
VneI GTGCAC 1 cut(s) 1032
VpaK11BI GGWCC 1 cut(s) 752
VspI ATTAAT 1 cut(s) 825
XagI CCTNNNNNAGG 1 cut(s) 1551
XapI RAATTY 5 cut(s) 1345, 1532, 1630, 1780, 2240
XceI RCATGY 3 cut(s) 1040, 1543, 1998
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 2063
XmnI GAANNNNTTC 2 cut(s) 165, 1783
ZraI GACGTC 1 cut(s) 1931
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.