MD06G1170300.v1.1

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013824
Physical Location & Seq
Reverse (-)
31094790 .. 31098796
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1170300.v1.1.491

Sequence Viewer

Length: 2454 bp
ATGGCGCCACCTAGTCCATCTGCTCCTGATCAATCCCAGCCGTCTTCGTCCTCGGACCCGAAAAGTAACAAGAAAGATTTTTCAACCGCCATTTTGGAACGAAAGAAGTCTCCTAATCGCCTTGTTGTCGATGAAGCTGTCAATGATGATAATTCTGTGATTTCCTTGCACCCTTCTGCAATGGAAAAGCTTCAGTTTTTCCGTGGAGACACTGTTTTAATTAAGGGGAAGAAAAGGAGAGACACAGTTTGCATTGTTATGGCTGATAACGAATGCGAGGAGCCGAAAATCAGAATGAACAAAGTTGTGAGAGCTAATCTTAGGGTTCGCCTCGGGGATATTGTGTCTGTTCATCCCTGCCCTGATGTCAAGTATGGAAAGAAGGTTCACATCCTTCCAATCGATGATACAATTGAAGGCGTGACAGGAAGCTTATTCGATGCATATTTGAAGCCATACTTTGCGGAAGCTTACCGCCCTGTGAGGAAGGGTGATCTTTTCCTAGTTCGGGGCATGATGCGAAGCATTGAGTTCAAGGTCATTGAGACAGACCCTGATGAGTACTGCATTGTTGCGCCGGATACTGAGATTTTCTGTGAGGGAGAGCCTGTCAAACGCGAGGACGAGGAAAGACTAGAGGATGTAGGATATGATGATGTTGGAGGTGTCAGAAAGCAAATGGCTCAGATACGCGAGTTAGTAGAACTGCCTCTTAGACATCCACAGCTCTTCAAATCGATTGGAGTGAAACCCCCGAAGGGGATTTTACTCTATGGGCCACCCGGGACTGGGAAAACATTGATTGCAAGGGCTGTAGCAAATGAGACTGGAGCGTTCTTCTTTTTTATCAATGGACCTGAAATAATGTCCAAGATGGCAGGTGAAAGTGAAAGCAACTTGAGGAAGGCGTTTCAGGAAGCTGAAAAGAATGCACCCTCAATCATTTTTATTGATGAGATAGATTCTATTGCTCCGAAGAGGGAAAAGACCCACGGTGAAGTGGAAAGGCGTATAGTTTCCCAGCTCCTGACTTTAATGGACGGCCTTAAGTCCCGTGCGCATGTCATTGTAATGGGAGCAACGAATAGGCCAAACAGCATCGACCCTGCTCTAAGGAGATTTGGAAGATTCGATAGAGAGATTGACATTGGTGTCCCGGATGAAGTTGGAAGATTGGAAGTTTTGCGAATCCATACAAAGAGGATGAAACTTGCAGATGATGTTGATCTTGAACGAGTTGCAAAAGATACCCATGGTTTTGTTGGTGCAGATCTTGCCGCTCTCTCCACAGAGGCTGCGCTCCAGTGCATCCGTGAAAAAATGGATGTTATTGACTTGGAAGACGATACGATTGATGCTGAGGTGTTGAATTCGATGGCTGTGACGAATGAACACTTCCAGACCGCACTGTCCTCTACAAATCCTTCTGCCTTGCGCGAAACTGTTGTGGAGGTACCTAATGTTCCGTGGGATGCCATTGGCGGGTTGGAGAACGTCAAAAGAGAACTTCAAGAGACAATTAAACTCCCCGTGGAGCATCCAGAGATGTTTGAGAAATTTGGGATGTCACCTTCTAAAGGCGTTCTCTTTTATGGACCACCTGGCTGTGGGAAAACCCTTCTCGCCAAGGCAATTGCCAATGAATGTCAGGCCAACTTCATCAGTATCAAAGGACCCGAGCTGCTAACAATGTGGTTCGGAGAAAGTGAGGCAAATGTGCGCGAGATATTTGACAAGGCTCGCCAATCTGCTCCTTGTGTTCTTTTCTTTGATGAGCTAGACTCAATTGCAACTCAGCGTGGCAATTCTGTGGGGGATGCTGGTGGTGCTGGAGACAGAGTCTTGAACCAACTTCTAACCGAAATGGATGGAATGAATGCAAAGAAAACCGTCTTTGTCATTGGGGCGACAAACAGGCCAGACATTATTGACCCGGCATTGCTCAGGCCTGGACGTCTTGACCAACTTATCTACATCCCGCTCCCAGATGAGGCTTCGCGCCTTCAGATTTTTAAAGCATGTCTGCGCAAGTCACCCATCTCTAAGGATATTGACCTAGCAGCTCTTGCCCGTTATACGCGCGGTTTTAGTGGTGCAGATATCACCGAGATATGTCAACGTGCCTGCAAATATGCCATCAGAGAAGACATTGAGAAGGATATTGAGAGGGAAAGAAGAAAGAATGATAACTCCGAAGCCATGGAGGAAGATGAAACTGATGAGGTACCCGAGATCAAGCCAGCGCACTTTGAGGAATCAATGAAACATGCCCGAAGAAGTGTGAGTGATGCAGACATCAAGAAGTACCAGGTATTTGCTCAGACTTTGCAGCAATCTCGTGGGTTTGGATCCGAATTTCGATTTCCAGAGACAGAGAACAATGGAGGAGAAGCAGCTCCAACCCCATTTTCTTCAGCCACAGTTGCTGGAGAGGAAGATGATCTCTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

818

Amino Acids

90.92

Weight (kDa)

5.14

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 40 - 121 1.7e-23 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 142 - 205 7.8e-12 Cell division protein 48 (CDC48), domain 2
RuvB_N PF05496 254 - 329 6.1e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 255 - 384 7.7e-49 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 407 - 448 5.1e-14 AAA+ lid domain
AAA PF00004 528 - 661 3.3e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 683 - 722 4.3e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 869
AasI GACNNNNNNGTC 2 cut(s) 1151, 1408
AatII GACGTC 1 cut(s) 1957
Acc16I TGCGCA 2 cut(s) 1059, 2027
Acc36I ACCTGC 1 cut(s) 869
Acc65I GGTACC 2 cut(s) 1453, 2224
AccB1I GGYRCC 3 cut(s) 4, 1453, 2224
AccBSI CCGCTC 2 cut(s) 1280, 1981
AccII CGCG 7 cut(s) 618, 693, 1437, 1722, 1999, 2080, 2082
AciI CCGC 8 cut(s) 87, 464, 475, 1278, 1404, 1482, 1979, 2082
AclWI GGATC 2 cut(s) 2343, 2356
AcsI RAATTY 3 cut(s) 1369, 1556, 2354
AcuI CTGAAG 3 cut(s) 176, 1988, 2397
AcyI GRCGYC 2 cut(s) 5, 1954
AfaI GTAC 4 cut(s) 563, 1455, 2226, 2306
AfiI CCNNNNNNNGG 9 cut(s) 508, 758, 759, 788, 789, 1482, 1577, 1607, 1990
AflII CTTAAG 1 cut(s) 1046
AgsI TTSAA 9 cut(s) 84, 416, 451, 535, 733, 1232, 1369, 1511, 1846
AjnI CCWGG 3 cut(s) 1600, 1948, 2307
AjuI GAANNNNNNNTTGG 2 cut(s) 2392, 2424
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
Alw26I GTCTC 8 cut(s) 114, 201, 234, 539, 818, 1508, 1827, 2363
AlwI GGATC 2 cut(s) 2343, 2356
AlwNI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2426
Ama87I CYCGRG 4 cut(s) 332, 782, 1676, 2228
AoxI GGCC 6 cut(s) 776, 1042, 1088, 1650, 1916, 1946
ApeKI GCWGC 5 cut(s) 1295, 1681, 2060, 2329, 2393
ApoI RAATTY 3 cut(s) 1369, 1556, 2354
Asp700I GAANNNNTTC 1 cut(s) 189
Asp718I GGTACC 2 cut(s) 1453, 2224
AspS9I GGNCC 5 cut(s) 55, 776, 854, 1595, 1673
AsuC2I CCSGG 4 cut(s) 783, 784, 1157, 1934
AsuHPI GGTGA 6 cut(s) 503, 893, 1007, 1560, 2025, 2095
AvaI CYCGRG 4 cut(s) 332, 782, 1676, 2228
AvaII GGWCC 4 cut(s) 55, 854, 1595, 1673
BaeI ACNNNNGTAYC 2 cut(s) 680, 713
BamHI GGATCC 1 cut(s) 2348
BanI GGYRCC 3 cut(s) 4, 1453, 2224
BauI CACGAG 1 cut(s) 2337
BbsI GAAGAC 3 cut(s) 36, 1347, 2151
BbvCI CCTCAGC 1 cut(s) 1359
BbvI GCAGC 5 cut(s) 1282, 1668, 2072, 2341, 2405
BccI CCATC 6 cut(s) 25, 868, 1369, 1862, 2045, 2144
BceAI ACGGC 2 cut(s) 25, 1057
BcgI CGANNNNNNTGC 2 cut(s) 2318, 2352
BciT130I CCWGG 3 cut(s) 1602, 1950, 2309
BciVI GTATCC 1 cut(s) 574
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 4 cut(s) 783, 784, 1157, 1934
BcoDI GTCTC 8 cut(s) 114, 201, 234, 539, 818, 1508, 1827, 2363
BfaI CTAG 5 cut(s) 12, 503, 635, 1778, 2057
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 1046
BfuAI ACCTGC 1 cut(s) 869
BfuI GTATCC 1 cut(s) 574
BglII AGATCT 1 cut(s) 1270
BisI GCNGC 6 cut(s) 1278, 1296, 1682, 2061, 2330, 2394
BlsI GCNGC 6 cut(s) 1279, 1297, 1683, 2062, 2331, 2395
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 7 cut(s) 783, 784, 1157, 1602, 1934, 1950, 2309
Bme18I GGWCC 4 cut(s) 55, 854, 1595, 1673
BmeT110I CYCGRG 4 cut(s) 332, 782, 1676, 2228
BmgT120I GGNCC 5 cut(s) 55, 776, 854, 1595, 1673
BmiI GGNNCC 7 cut(s) 6, 57, 282, 1455, 1675, 2226, 2350
BmrFI CCNGG 7 cut(s) 783, 784, 1157, 1602, 1934, 1950, 2309
BmrI ACTGGG 1 cut(s) 798
BmsI GCATC 9 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1546, 1807, 2278
BmuI ACTGGG 1 cut(s) 798
BpiI GAAGAC 3 cut(s) 36, 1347, 2151
BplI GAGNNNNNCTC 2 cut(s) 1766, 1798
BpmI CTGGAG 4 cut(s) 849, 1286, 1851, 2448
Bpu10I CCTNAGC 2 cut(s) 1359, 1943
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 4 cut(s) 783, 784, 1157, 1934
Bsa29I ATCGAT 2 cut(s) 402, 737
BsaBI GATNNNNATC 1 cut(s) 1224
BsaHI GRCGYC 2 cut(s) 5, 1954
BsaXI ACNNNNNCTCC 6 cut(s) 198, 228, 594, 624, 1824, 1854
Bsc4I CCNNNNNNNGG 9 cut(s) 508, 758, 759, 788, 789, 1482, 1577, 1607, 1990
Bse1I ACTGG 3 cut(s) 793, 832, 1303
Bse3DI GCAATG 2 cut(s) 186, 1937
Bse8I GATNNNNATC 1 cut(s) 1224
BseBI CCWGG 3 cut(s) 1602, 1950, 2309
BseCI ATCGAT 2 cut(s) 402, 737
BseJI GATNNNNATC 1 cut(s) 1224
BseLI CCNNNNNNNGG 9 cut(s) 508, 758, 759, 788, 789, 1482, 1577, 1607, 1990
BseMI GCAATG 2 cut(s) 186, 1937
BseMII CTCAG 6 cut(s) 576, 698, 1350, 1808, 1957, 2333
BseNI ACTGG 3 cut(s) 793, 832, 1303
BseRI GAGGAG 2 cut(s) 293, 2400
BseXI GCAGC 5 cut(s) 1282, 1668, 2072, 2341, 2405
BseYI CCCAGC 2 cut(s) 36, 1020
BsgI GTGCAG 2 cut(s) 1287, 2115
Bsh1236I CGCG 7 cut(s) 618, 693, 1437, 1722, 1999, 2080, 2082
BshFI GGCC 6 cut(s) 778, 1044, 1090, 1652, 1918, 1948
BshNI GGYRCC 3 cut(s) 4, 1453, 2224
BshVI ATCGAT 2 cut(s) 402, 737
BsiHKCI CYCGRG 4 cut(s) 332, 782, 1676, 2228
BsiSI CCGG 4 cut(s) 578, 783, 1157, 1934
BslFI GGGAC 3 cut(s) 799, 1036, 1139
BslI CCNNNNNNNGG 9 cut(s) 508, 758, 759, 788, 789, 1482, 1577, 1607, 1990
BsmAI GTCTC 8 cut(s) 114, 201, 234, 539, 818, 1508, 1827, 2363
BsmFI GGGAC 3 cut(s) 799, 1036, 1139
BsmI GAATGC 3 cut(s) 278, 934, 1882
BsnI GGCC 6 cut(s) 778, 1044, 1090, 1652, 1918, 1948
BsoBI CYCGRG 4 cut(s) 332, 782, 1676, 2228
Bsp143I GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2440
Bsp19I CCATGG 2 cut(s) 1252, 2199
BspACI CCGC 8 cut(s) 87, 464, 475, 1278, 1404, 1482, 1979, 2082
BspANI GGCC 6 cut(s) 778, 1044, 1090, 1652, 1918, 1948
BspCNI CTCAG 6 cut(s) 577, 697, 1351, 1807, 1956, 2332
BspDI ATCGAT 2 cut(s) 402, 737
BspFNI CGCG 7 cut(s) 618, 693, 1437, 1722, 1999, 2080, 2082
BspLI GGNNCC 7 cut(s) 6, 57, 282, 1455, 1675, 2226, 2350
BspMI ACCTGC 1 cut(s) 869
BspPI GGATC 2 cut(s) 2343, 2356
BspQI GCTCTTC 1 cut(s) 734
BspT107I GGYRCC 3 cut(s) 4, 1453, 2224
BspTI CTTAAG 1 cut(s) 1046
BsrBI CCGCTC 2 cut(s) 1280, 1981
BsrDI GCAATG 2 cut(s) 186, 1937
BsrI ACTGG 3 cut(s) 793, 832, 1303
BssMI GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2440
BssNI GRCGYC 2 cut(s) 5, 1954
BssSI CACGAG 1 cut(s) 2337
BssT1I CCWWGG 3 cut(s) 1252, 1626, 2199
Bst2BI CACGAG 1 cut(s) 2337
Bst2UI CCWGG 3 cut(s) 1602, 1950, 2309
Bst4CI ACNGT 7 cut(s) 214, 247, 995, 1410, 1444, 1891, 2422
Bst6I CTCTTC 2 cut(s) 734, 971
BstACI GRCGYC 2 cut(s) 5, 1954
BstAFI CTTAAG 1 cut(s) 1046
BstAPI GCANNNNNTGC 2 cut(s) 1274, 2066
BstC8I GCNNGC 3 cut(s) 1741, 2125, 2241
BstDSI CCRYGG 6 cut(s) 202, 991, 1252, 1466, 1530, 2199
BstENI CCTNNNNNAGG 1 cut(s) 1575
BstFNI CGCG 7 cut(s) 618, 693, 1437, 1722, 1999, 2080, 2082
BstH2I RGCGCY 1 cut(s) 8
BstKTI GATC 7 cut(s) 31, 496, 1228, 1273, 2235, 2351, 2443
BstMAI GTCTC 8 cut(s) 114, 201, 234, 539, 818, 1508, 1827, 2363
BstMBI GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2440
BstMWI GCNNNNNNNGC 4 cut(s) 1274, 1826, 2066, 2423
BstNI CCWGG 3 cut(s) 1602, 1950, 2309
BstNSI RCATGY 3 cut(s) 1064, 2022, 2270
BstSCI CCNGG 7 cut(s) 781, 782, 1155, 1600, 1932, 1948, 2307
BstSFI CTRYAG 1 cut(s) 813
BstUI CGCG 7 cut(s) 618, 693, 1437, 1722, 1999, 2080, 2082
BstV1I GCAGC 5 cut(s) 1282, 1668, 2072, 2341, 2405
BstV2I GAAGAC 3 cut(s) 36, 1347, 2151
BstX2I RGATCY 2 cut(s) 1270, 2348
BstYI RGATCY 2 cut(s) 1270, 2348
Bsu15I ATCGAT 2 cut(s) 402, 737
BsuI GTATCC 1 cut(s) 574
BsuRI GGCC 6 cut(s) 778, 1044, 1090, 1652, 1918, 1948
BsuTUI ATCGAT 2 cut(s) 402, 737
BtgI CCRYGG 6 cut(s) 202, 991, 1252, 1466, 1530, 2199
BtsIMutI CAGTG 3 cut(s) 210, 1310, 1406
BveI ACCTGC 1 cut(s) 869
Cac8I GCNNGC 3 cut(s) 1741, 2125, 2241
CaiI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2426
Cfr13I GGNCC 5 cut(s) 55, 776, 854, 1595, 1673
Cfr9I CCCGGG 1 cut(s) 782
ClaI ATCGAT 2 cut(s) 402, 737
CsiI ACCWGGT 1 cut(s) 2307
Csp6I GTAC 4 cut(s) 562, 1454, 2225, 2305
CviAII CATG 6 cut(s) 514, 1061, 1253, 2019, 2200, 2267
CviQI GTAC 4 cut(s) 562, 1454, 2225, 2305
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 30, 495, 1227, 1272, 2234, 2350, 2442
DpnII GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2440
DraI TTTAAA 1 cut(s) 2014
DrdI GACNNNNNNGTC 2 cut(s) 1151, 1408
DseDI GACNNNNNNGTC 2 cut(s) 1151, 1408
Eam1104I CTCTTC 2 cut(s) 734, 971
EarI CTCTTC 2 cut(s) 734, 971
Eco130I CCWWGG 3 cut(s) 1252, 1626, 2199
Eco147I AGGCCT 1 cut(s) 1948
Eco32I GATATC 1 cut(s) 2101
Eco47I GGWCC 4 cut(s) 55, 854, 1595, 1673
Eco57I CTGAAG 3 cut(s) 176, 1988, 2397
Eco88I CYCGRG 4 cut(s) 332, 782, 1676, 2228
EcoNI CCTNNNNNAGG 1 cut(s) 1575
EcoO109I RGGNCCY 1 cut(s) 1673
EcoRI GAATTC 1 cut(s) 1369
EcoRII CCWGG 3 cut(s) 1600, 1948, 2307
EcoRV GATATC 1 cut(s) 2101
EcoT14I CCWWGG 3 cut(s) 1252, 1626, 2199
EcoT22I ATGCAT 1 cut(s) 445
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 1252, 1626, 2199
FaeI CATG 6 cut(s) 517, 1064, 1256, 2022, 2203, 2270
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 3 cut(s) 799, 1036, 1139
FatI CATG 6 cut(s) 513, 1060, 1252, 2018, 2199, 2266
FauI CCCGC 2 cut(s) 1475, 1986
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 6 cut(s) 1278, 1296, 1682, 2061, 2330, 2394
Fsp4HI GCNGC 6 cut(s) 1278, 1296, 1682, 2061, 2330, 2394
FspAI RTGCGCAY 1 cut(s) 1059
FspBI CTAG 5 cut(s) 12, 503, 635, 1778, 2057
FspI TGCGCA 2 cut(s) 1059, 2027
GluI GCNGC 6 cut(s) 1278, 1296, 1682, 2061, 2330, 2394
GsaI CCCAGC 2 cut(s) 40, 1024
GsuI CTGGAG 4 cut(s) 849, 1286, 1851, 2448
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 778, 1044, 1090, 1652, 1918, 1948
HapII CCGG 4 cut(s) 578, 783, 1157, 1934
Hin1I GRCGYC 2 cut(s) 5, 1954
Hin1II CATG 6 cut(s) 517, 1064, 1256, 2022, 2203, 2270
HincII GTYRAC 1 cut(s) 2117
HindII GTYRAC 1 cut(s) 2117
HindIII AAGCTT 3 cut(s) 188, 430, 468
HinfI GANTC 6 cut(s) 962, 1128, 1188, 1781, 1839, 2255
HpaII CCGG 4 cut(s) 578, 783, 1157, 1934
HphI GGTGA 6 cut(s) 503, 893, 1007, 1560, 2025, 2095
Hpy166II GTNNAC 2 cut(s) 388, 2117
Hpy8I GTNNAC 2 cut(s) 388, 2117
HpyCH4III ACNGT 7 cut(s) 214, 247, 995, 1410, 1444, 1891, 2422
HpyCH4IV ACGT 3 cut(s) 1494, 1954, 2119
HpyF10VI GCNNNNNNNGC 4 cut(s) 1274, 1826, 2066, 2423
HpySE526I ACGT 3 cut(s) 1494, 1954, 2119
Hsp92I GRCGYC 2 cut(s) 5, 1954
Hsp92II CATG 6 cut(s) 517, 1064, 1256, 2022, 2203, 2270
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 2 cut(s) 1457, 2228
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2440
LguI GCTCTTC 1 cut(s) 734
Lsp1109I GCAGC 5 cut(s) 1282, 1668, 2072, 2341, 2405
LweI GCATC 9 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1546, 1807, 2278
MabI ACCWGGT 1 cut(s) 2307
MaeI CTAG 5 cut(s) 12, 503, 635, 1778, 2057
MaeII ACGT 3 cut(s) 1494, 1954, 2119
MaeIII GTNAC 5 cut(s) 65, 421, 1381, 1566, 2031
MalI GATC 7 cut(s) 30, 495, 1227, 1272, 2234, 2350, 2442
MbiI CCGCTC 2 cut(s) 1280, 1981
MboI GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2440
MfeI CAATTG 3 cut(s) 411, 1632, 1785
MflI RGATCY 2 cut(s) 1270, 2348
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 1775, 1848
MmeI TCCRAC 4 cut(s) 640, 1147, 1467, 2423
Mph1103I ATGCAT 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 189
MseI TTAA 6 cut(s) 218, 222, 1034, 1047, 1521, 2013
MslI CAYNNNNRTG 2 cut(s) 257, 1070
MspCI CTTAAG 1 cut(s) 1046
MspI CCGG 4 cut(s) 578, 783, 1157, 1934
MspR9I CCNGG 7 cut(s) 783, 784, 1157, 1602, 1934, 1950, 2309
MunI CAATTG 3 cut(s) 411, 1632, 1785
Mva1269I GAATGC 3 cut(s) 278, 934, 1882
MvaI CCWGG 3 cut(s) 1602, 1950, 2309
MvnI CGCG 7 cut(s) 618, 693, 1437, 1722, 1999, 2080, 2082
MwoI GCNNNNNNNGC 4 cut(s) 1274, 1826, 2066, 2423
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 783, 784, 1157, 1934
NcoI CCATGG 2 cut(s) 1252, 2199
NdeII GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2440
NlaIII CATG 6 cut(s) 517, 1064, 1256, 2022, 2203, 2270
NlaIV GGNNCC 7 cut(s) 6, 57, 282, 1455, 1675, 2226, 2350
NmuCI GTSAC 4 cut(s) 421, 1381, 1566, 2031
NsbI TGCGCA 2 cut(s) 1059, 2027
NsiI ATGCAT 1 cut(s) 445
NspI RCATGY 3 cut(s) 1064, 2022, 2270
PacI TTAATTAA 1 cut(s) 222
PaqCI CACCTGC 1 cut(s) 869
PceI AGGCCT 1 cut(s) 1948
PciSI GCTCTTC 1 cut(s) 734
PctI GAATGC 3 cut(s) 278, 934, 1882
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 4 cut(s) 962, 1128, 1188, 2255
PflFI GACNNNGTC 1 cut(s) 1838
PfoI TCCNGGA 1 cut(s) 1155
PkrI GCNGC 6 cut(s) 1279, 1297, 1683, 2062, 2331, 2395
PleI GAGTC 2 cut(s) 1775, 1847
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 1775, 1847
PpuMI RGGWCCY 1 cut(s) 1673
Psp5II RGGWCCY 1 cut(s) 1673
Psp6I CCWGG 3 cut(s) 1600, 1948, 2307
PspFI CCCAGC 2 cut(s) 36, 1020
PspGI CCWGG 3 cut(s) 1600, 1948, 2307
PspN4I GGNNCC 7 cut(s) 6, 57, 282, 1455, 1675, 2226, 2350
PspPI GGNCC 5 cut(s) 55, 776, 854, 1595, 1673
PspPPI RGGWCCY 1 cut(s) 1673
PsrI GAACNNNNNNTAC 2 cut(s) 1446, 1478
PstNI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2426
PsuI RGATCY 2 cut(s) 1270, 2348
PsyI GACNNNGTC 1 cut(s) 1838
RsaI GTAC 4 cut(s) 563, 1455, 2226, 2306
RsaNI GTAC 4 cut(s) 562, 1454, 2225, 2305
RseI CAYNNNNRTG 2 cut(s) 257, 1070
SapI GCTCTTC 1 cut(s) 734
SaqAI TTAA 6 cut(s) 218, 222, 1034, 1047, 1521, 2013
SatI GCNGC 6 cut(s) 1278, 1296, 1682, 2061, 2330, 2394
Sau3AI GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2440
Sau96I GGNCC 5 cut(s) 55, 776, 854, 1595, 1673
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 2 cut(s) 1775, 1848
ScrFI CCNGG 7 cut(s) 783, 784, 1157, 1602, 1934, 1950, 2309
SexAI ACCWGGT 1 cut(s) 2307
SfaNI GCATC 9 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1546, 1807, 2278
SfcI CTRYAG 1 cut(s) 813
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 4 cut(s) 55, 854, 1595, 1673
SmaI CCCGGG 1 cut(s) 784
SmiMI CAYNNNNRTG 2 cut(s) 257, 1070
SmlI CTYRAG 2 cut(s) 898, 1046
SmoI CTYRAG 2 cut(s) 898, 1046
SseBI AGGCCT 1 cut(s) 1948
SsiI CCGC 8 cut(s) 87, 464, 475, 1278, 1404, 1482, 1979, 2082
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 5 cut(s) 12, 503, 635, 1778, 2057
StuI AGGCCT 1 cut(s) 1948
StyD4I CCNGG 7 cut(s) 781, 782, 1155, 1600, 1932, 1948, 2307
StyI CCWWGG 3 cut(s) 1252, 1626, 2199
TaaI ACNGT 7 cut(s) 214, 247, 995, 1410, 1444, 1891, 2422
TaiI ACGT 3 cut(s) 1497, 1957, 2122
TaqI TCGA 8 cut(s) 129, 402, 438, 737, 1101, 1131, 1373, 2359
TatI WGTACW 1 cut(s) 561
TauI GCSGC 1 cut(s) 1280
TfiI GAWTC 4 cut(s) 962, 1128, 1188, 2255
Tru1I TTAA 6 cut(s) 218, 222, 1034, 1047, 1521, 2013
Tru9I TTAA 6 cut(s) 218, 222, 1034, 1047, 1521, 2013
TscAI CASTG 3 cut(s) 217, 1310, 1413
TseFI GTSAC 4 cut(s) 421, 1381, 1566, 2031
TseI GCWGC 5 cut(s) 1295, 1681, 2060, 2329, 2393
Tsp45I GTSAC 4 cut(s) 421, 1381, 1566, 2031
TspGWI ACGGA 3 cut(s) 191, 1301, 1455
TspMI CCCGGG 1 cut(s) 782
TspRI CASTG 3 cut(s) 217, 1310, 1413
Tth111I GACNNNGTC 1 cut(s) 1838
Vha464I CTTAAG 1 cut(s) 1046
VpaK11BI GGWCC 4 cut(s) 55, 854, 1595, 1673
XagI CCTNNNNNAGG 1 cut(s) 1575
XapI RAATTY 3 cut(s) 1369, 1556, 2354
XceI RCATGY 3 cut(s) 1064, 2022, 2270
XcmI CCANNNNNNNNNTGG 2 cut(s) 1259, 1483
XmaI CCCGGG 1 cut(s) 782
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 5 cut(s) 12, 503, 635, 1778, 2057
ZraI GACGTC 1 cut(s) 1955
ZrmI AGTACT 1 cut(s) 563
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.