Rw7G011310

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013824
Physical Location & Seq
Reverse (-)
10596905 .. 10600758
3854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011310.1

Sequence Viewer

Length: 2406 bp
ATGGCGGATCCGAGCTCATCTGCCCCAAATCCTGACCCGAAAACTAAGAAGGATTTTTCCACTGCAATTTTGGAGCGAAAGAAGTCTCCTAATCGACTTGTGGTTGATGAAGCTATCAATGATGATAATTCTGTGGTGTCCTTGCACCCTGCTACCATGGAAAAACTTCAGTTTTTTCGGGGTGACACTGTGCTAATAAAGGGGAAGAAACGAAAAGACACAGTTTGCATTGTTCTTGCTGATGAACAATGTGAAGAACCGAAAATCAGAATGAACAAAGTTGTAAGGGCTAATCTTAGGGTTCGTCTTGGAGATGTTGTGTCGGTTCATACTTGCCCTGATGTCAAATATGGGAAGCGGGTTCACATCCTTCCAATCGATGATACAATTGAAGGGTGTAACGGAAACTTGTTTGATGCATATTTGAAGCCATACTTCTTGGAATCTTACCGGCCTGTGAGGAAGGGTGACCTTTTCCTGGTCAGGGGTGGGATGCGAAGTGTTGAGTTCAAGGTCATTGAGACAGACCCTGGTGAATACTGTGTTGTTGCTCCCGATACTGAGATTTTCTGTGAGGGAGAACCTGTGAAACGTGAGGATGAGGAAAAATTGAATGAGGTGGGCTATGATGATGTTGGAGGTGTTAGGAAGCAAATGGCTCAGATACGCGAGTTAGTAGAGTTACCTCTTAGACACCCACAGCTCTTCAAGTCCATTGGAGTGAAACCACCTAAAGGTATTCTGCTTTATGGACCACCTGGGTCTGGGAAAACTTTGATTGCAAGGGCTGTAGCTAATGAGACTGGAGCATTCTTCTTTTTGATTAATGGGCCTGAAATAATGTCAAAGTTAGCTGGTGAGAGTGAAAGCAACTTGAGGAAGGCATTTGAAGAAGCTGAAAAGAATGCCCCATCAATCATTTTTATTGATGAGTTAGATTCCATTGCTCCAAAGAGGGAGAAGACTCAGGGTGAAGTGGAAAGGCGTATAGTATCGCAACTTCTGACTTTGATGGATGGTCTCAAGTCCCGTGCACATGTTATTGTCATGGGAGCTACAAATAGGCCAAACAGCATTGACCCTGCTCTGAGGAGGTTTGGAAGATTTGATAGAGAGATTGACATAGGTGTACCGGATGAAGTTGGAAGATTGGAAGTTTTGAGAATCCATACAAAGAACATGAAACTTGCAGAAGATGATACCCATGGTTATGTCGGTGCTGATCTTGCCGCTCTCTGTACTGAGGCTGCTCTTCAGTGCATCCGTGAGAAAATGGATGTTATTGACTTGGAAGATGAGGAAATTGATGCTGAGGTGTTGAATTCTATGGCTGTGACGAATGAACACTTCCAGACTGCATTGGGTGCTTCAAATCCTTCAGCATTGCGTGAAACTGTTGTGGAGGTACCTAATGTCTCATGGGACGCTATTGGTGGATTGGACAATGTCAAAAGAGAACTTCAAGAGACTGTTCAATACCCAGTGGAGCATCCTGAGAAGTTTGAGAAATTTGGCATGTCACCTTCTAAAGGTGTTCTCTTTTATGGGCCACCGGGCTGTGGTAAAACCCTTCTTGCTAAGGCAATTGCCAATGAATGCCAGGCGAATTTCATTAGCATCAAAGGCCCCGAGTTACTGACAATGTGGTTTGGAGAAAGTGAGGCAAACGTCCGTGAGATATTTGACAAGGCTCGCCAATCTGCTCCTTGTGTTCTTTTCTTTGATGAGCTGGACTCGATTGCAACTCAGCGTGGAAATTCTTCTGGAGATGCTGGTGGTGCTGCAGATAGAGTCTTGAACCAACTTCTAACTGAAATGGATGGAATGAGTGCAAAGAAAACAGTTTTTATCATTGGAGCAACAAACAGGCCAGACATTATTGACCCAGCATTGCTCAGGCCTGGACGTCTTGACCAGCTCATCTATATCCCACTCCCAGATGAAGCTTCCCGTCTTCAGATTTTCAAAGCATGTTTGCGGAAGTCACCCGTCTCTAAGGATGTTGACCTTGCAGTTCTTGCGCATCATACCCATGGTTTTAGTGGTGCTGATATCACTGAGATATGTCAGCGTGCCTGCAAATATGCCATCAGAGAAAATATTGAGAAGGATATTGAGAAGGAGAGAAGAAGGGCAGAGAACCCAGAAGCGATGGAGGAGGATGACATTGATGAGGTTCCAGAGATAAAGGCAGCACACTTCGAGGAATCCATGAAATTTGCCCGCCGAAGTGTTAGTGATGCTGACATCAGGAAGTACCAGGCATTCGCTCAGACTCTGCAGCAATCTCGTGGGCTTGGATCCGACTTCCGGTTCCCCCAAACGGGCACCACTGCAGGAGGAGGAGCTCCAGACCCTTTTGCTTCGGCCCCAGCTGCTGCAGATGACGATGATCTGTACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

801

Amino Acids

88.65

Weight (kDa)

5.16

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 32 - 113 1.2e-23 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 135 - 197 2.2e-11 Cell division protein 48 (CDC48), domain 2
AAA PF00004 247 - 376 1.4e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 395 - 434 2.9e-12 AAA+ lid domain
AAA PF00004 512 - 645 1.1e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 667 - 707 4.2e-13 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1909
Acc16I TGCGCA 1 cut(s) 2022
Acc65I GGTACC 1 cut(s) 1405
AccB1I GGYRCC 2 cut(s) 1405, 2327
AccBSI CCGCTC 1 cut(s) 1232
AccII CGCG 1 cut(s) 669
AciI CCGC 5 cut(s) 5, 358, 1230, 1978, 2224
AclWI GGATC 4 cut(s) 2, 15, 2295, 2308
AcsI RAATTY 5 cut(s) 1321, 1508, 1606, 1756, 2216
AcuI CTGAAG 4 cut(s) 152, 1238, 1362, 1940
AcyI GRCGYC 1 cut(s) 1906
AfaI GTAC 5 cut(s) 1131, 1240, 1407, 2258, 2399
AfiI CCNNNNNNNGG 9 cut(s) 478, 484, 734, 764, 1529, 1559, 2310, 2323, 2324
AflIII ACRYGT 1 cut(s) 1036
AjnI CCWGG 6 cut(s) 477, 529, 757, 1599, 1900, 2259
Alw21I GWGCWC 3 cut(s) 17, 1036, 2350
Alw26I GTCTC 7 cut(s) 90, 515, 794, 1025, 1420, 1460, 1996
Alw44I GTGCAC 1 cut(s) 1032
AlwI GGATC 4 cut(s) 2, 15, 2295, 2308
AlwNI CAGNNNCTG 3 cut(s) 530, 2278, 2378
Ama87I CYCGRG 1 cut(s) 1628
AoxI GGCC 8 cut(s) 452, 830, 1064, 1547, 1624, 1868, 1898, 2367
ApaLI GTGCAC 1 cut(s) 1032
ApeKI GCWGC 6 cut(s) 1247, 1781, 2192, 2281, 2375, 2378
ApoI RAATTY 5 cut(s) 1321, 1508, 1606, 1756, 2216
AseI ATTAAT 1 cut(s) 825
Asp700I GAANNNNTTC 2 cut(s) 165, 1759
Asp718I GGTACC 1 cut(s) 1405
AspLEI GCGC 1 cut(s) 2023
AspS9I GGNCC 5 cut(s) 752, 830, 1547, 1625, 2368
AsuC2I CCSGG 1 cut(s) 1554
AsuHPI GGTGA 7 cut(s) 194, 479, 545, 869, 983, 1512, 1977
AvaI CYCGRG 1 cut(s) 1628
AvaII GGWCC 1 cut(s) 752
BaeGI GKGCMC 2 cut(s) 1036, 2330
BaeI ACNNNNGTAYC 2 cut(s) 656, 689
BamHI GGATCC 2 cut(s) 7, 2300
BanI GGYRCC 2 cut(s) 1405, 2327
BanII GRGCYC 2 cut(s) 17, 2350
BarI GAAGNNNNNNTAC 2 cut(s) 984, 1016
BauI CACGAG 1 cut(s) 2289
BbsI GAAGAC 2 cut(s) 968, 1946
Bbv12I GWGCWC 3 cut(s) 17, 1036, 2350
BbvCI CCTCAGC 1 cut(s) 1311
BbvI GCAGC 6 cut(s) 1234, 1768, 2204, 2293, 2362, 2365
BccI CCATC 6 cut(s) 919, 1006, 1010, 1814, 2096, 2146
BcgI CGANNNNNNTGC 2 cut(s) 2270, 2304
BciT130I CCWGG 6 cut(s) 479, 531, 759, 1601, 1902, 2261
BcnI CCSGG 1 cut(s) 1554
BcoDI GTCTC 7 cut(s) 90, 515, 794, 1025, 1420, 1460, 1996
BfmI CTRYAG 5 cut(s) 789, 1782, 2279, 2334, 2379
BisI GCNGC 7 cut(s) 1230, 1248, 1782, 2193, 2282, 2376, 2379
BlsI GCNGC 7 cut(s) 1231, 1249, 1783, 2194, 2283, 2377, 2380
Bme1390I CCNGG 7 cut(s) 479, 531, 759, 1554, 1601, 1902, 2261
Bme18I GGWCC 1 cut(s) 752
BmeT110I CYCGRG 1 cut(s) 1628
BmgT120I GGNCC 5 cut(s) 752, 830, 1547, 1625, 2368
BmiI GGNNCC 8 cut(s) 9, 1407, 1627, 2178, 2302, 2315, 2329, 2370
BmrFI CCNGG 7 cut(s) 479, 531, 759, 1554, 1601, 1902, 2261
BmrI ACTGGG 1 cut(s) 1475
BmsI GCATC 9 cut(s) 406, 483, 1269, 1297, 1498, 1626, 1759, 2032, 2230
BmuI ACTGGG 1 cut(s) 1475
BpiI GAAGAC 2 cut(s) 968, 1946
BplI GAGNNNNNCTC 2 cut(s) 1718, 1750
BpmI CTGGAG 3 cut(s) 825, 1785, 2334
Bpu10I CCTNAGC 3 cut(s) 1311, 1578, 1895
BpuEI CTTGAG 2 cut(s) 895, 1007
BpuMI CCSGG 1 cut(s) 1554
Bsa29I ATCGAT 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 1906
BsaI GGTCTC 1 cut(s) 1025
BsaJI CCNNGG 5 cut(s) 156, 529, 758, 1204, 2032
BsaWI WCCGGW 2 cut(s) 1132, 2310
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 570, 600
Bsc4I CCNNNNNNNGG 9 cut(s) 478, 484, 734, 764, 1529, 1559, 2310, 2323, 2324
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 2 cut(s) 808, 1481
Bse3DI GCAATG 3 cut(s) 942, 1382, 1889
BseBI CCWGG 6 cut(s) 479, 531, 759, 1601, 1902, 2261
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 5 cut(s) 156, 529, 758, 1204, 2032
BseLI CCNNNNNNNGG 9 cut(s) 478, 484, 734, 764, 1529, 1559, 2310, 2323, 2324
BseMI GCAATG 3 cut(s) 942, 1382, 1889
BseNI ACTGG 2 cut(s) 808, 1481
BseRI GAGGAG 4 cut(s) 1105, 2171, 2355, 2358
BseSI GKGCMC 2 cut(s) 1036, 2330
BseXI GCAGC 6 cut(s) 1234, 1768, 2204, 2293, 2362, 2365
BseYI CCCAGC 2 cut(s) 1885, 2371
Bsh1236I CGCG 1 cut(s) 669
BshFI GGCC 8 cut(s) 454, 832, 1066, 1549, 1626, 1870, 1900, 2369
BshNI GGYRCC 2 cut(s) 1405, 2327
BshVI ATCGAT 1 cut(s) 378
BsiHKAI GWGCWC 3 cut(s) 17, 1036, 2350
BsiHKCI CYCGRG 1 cut(s) 1628
BsiSI CCGG 4 cut(s) 451, 1133, 1553, 2311
BslFI GGGAC 2 cut(s) 1012, 1436
BslI CCNNNNNNNGG 9 cut(s) 478, 484, 734, 764, 1529, 1559, 2310, 2323, 2324
BsmAI GTCTC 7 cut(s) 90, 515, 794, 1025, 1420, 1460, 1996
BsmBI CGTCTC 1 cut(s) 1996
BsmFI GGGAC 2 cut(s) 1012, 1436
BsmI GAATGC 4 cut(s) 809, 910, 1601, 2264
BsnI GGCC 8 cut(s) 454, 832, 1066, 1549, 1626, 1870, 1900, 2369
Bso31I GGTCTC 1 cut(s) 1025
BsoBI CYCGRG 1 cut(s) 1628
Bsp1286I GDGCHC 4 cut(s) 17, 1036, 2330, 2350
Bsp1407I TGTACA 1 cut(s) 2397
Bsp143I GATC 4 cut(s) 7, 1222, 2300, 2392
Bsp19I CCATGG 3 cut(s) 156, 1204, 2032
BspACI CCGC 5 cut(s) 5, 358, 1230, 1978, 2224
BspANI GGCC 8 cut(s) 454, 832, 1066, 1549, 1626, 1870, 1900, 2369
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 1 cut(s) 669
BspLI GGNNCC 8 cut(s) 9, 1407, 1627, 2178, 2302, 2315, 2329, 2370
BspMAI CTGCAG 4 cut(s) 1786, 2283, 2338, 2383
BspPI GGATC 4 cut(s) 2, 15, 2295, 2308
BspQI GCTCTTC 2 cut(s) 710, 1257
BspT107I GGYRCC 2 cut(s) 1405, 2327
BspTNI GGTCTC 1 cut(s) 1025
BsrBI CCGCTC 1 cut(s) 1232
BsrDI GCAATG 3 cut(s) 942, 1382, 1889
BsrFI RCCGGY 1 cut(s) 450
BsrGI TGTACA 1 cut(s) 2397
BsrI ACTGG 2 cut(s) 808, 1481
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 5 cut(s) 156, 529, 758, 1204, 2032
BssMI GATC 4 cut(s) 7, 1222, 2300, 2392
BssNI GRCGYC 1 cut(s) 1906
BssSI CACGAG 1 cut(s) 2289
BssT1I CCWWGG 3 cut(s) 156, 1204, 2032
Bst2BI CACGAG 1 cut(s) 2289
Bst2UI CCWGG 6 cut(s) 479, 531, 759, 1601, 1902, 2261
Bst4CI ACNGT 6 cut(s) 190, 223, 542, 1396, 1471, 1843
Bst6I CTCTTC 2 cut(s) 710, 1257
BstACI GRCGYC 1 cut(s) 1906
BstAPI GCANNNNNTGC 2 cut(s) 1364, 2018
BstAUI TGTACA 1 cut(s) 2397
BstC8I GCNNGC 4 cut(s) 1693, 2073, 2077, 2224
BstDSI CCRYGG 3 cut(s) 156, 1204, 2032
BstEII GGTNACC 1 cut(s) 467
BstENI CCTNNNNNAGG 1 cut(s) 1527
BstFNI CGCG 1 cut(s) 669
BstHHI GCGC 1 cut(s) 2023
BstKTI GATC 4 cut(s) 10, 1225, 2303, 2395
BstMAI GTCTC 7 cut(s) 90, 515, 794, 1025, 1420, 1460, 1996
BstMBI GATC 4 cut(s) 7, 1222, 2300, 2392
BstMWI GCNNNNNNNGC 6 cut(s) 1226, 1364, 1623, 1778, 2018, 2375
BstNI CCWGG 6 cut(s) 479, 531, 759, 1601, 1902, 2261
BstNSI RCATGY 3 cut(s) 1040, 1519, 1974
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 7 cut(s) 477, 529, 757, 1552, 1599, 1900, 2259
BstSFI CTRYAG 5 cut(s) 789, 1782, 2279, 2334, 2379
BstSLI GKGCMC 2 cut(s) 1036, 2330
BstUI CGCG 1 cut(s) 669
BstV1I GCAGC 6 cut(s) 1234, 1768, 2204, 2293, 2362, 2365
BstV2I GAAGAC 2 cut(s) 968, 1946
BstX2I RGATCY 2 cut(s) 7, 2300
BstYI RGATCY 2 cut(s) 7, 2300
Bsu15I ATCGAT 1 cut(s) 378
BsuRI GGCC 8 cut(s) 454, 832, 1066, 1549, 1626, 1870, 1900, 2369
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 3 cut(s) 156, 1204, 2032
BtgZI GCGATG 1 cut(s) 2165
BtsI GCAGTG 2 cut(s) 60, 2331
BtsIMutI CAGTG 6 cut(s) 60, 186, 1262, 1488, 2055, 2331
Cac8I GCNNGC 4 cut(s) 1693, 2073, 2077, 2224
CaiI CAGNNNCTG 3 cut(s) 530, 2278, 2378
CfoI GCGC 1 cut(s) 2023
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 5 cut(s) 752, 830, 1547, 1625, 2368
ClaI ATCGAT 1 cut(s) 378
CseI GACGC 1 cut(s) 1433
Csp6I GTAC 5 cut(s) 1130, 1239, 1406, 2257, 2398
CviQI GTAC 5 cut(s) 1130, 1239, 1406, 2257, 2398
DpnI GATC 4 cut(s) 9, 1224, 2302, 2394
DpnII GATC 4 cut(s) 7, 1222, 2300, 2392
Eam1104I CTCTTC 2 cut(s) 710, 1257
EarI CTCTTC 2 cut(s) 710, 1257
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 15, 2348
Eco130I CCWWGG 3 cut(s) 156, 1204, 2032
Eco147I AGGCCT 1 cut(s) 1900
Eco24I GRGCYC 2 cut(s) 17, 2350
Eco31I GGTCTC 1 cut(s) 1025
Eco32I GATATC 1 cut(s) 2053
Eco47I GGWCC 1 cut(s) 752
Eco53kI GAGCTC 2 cut(s) 15, 2348
Eco57I CTGAAG 4 cut(s) 152, 1238, 1362, 1940
Eco88I CYCGRG 1 cut(s) 1628
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 2 cut(s) 15, 2348
EcoNI CCTNNNNNAGG 1 cut(s) 1527
EcoO109I RGGNCCY 1 cut(s) 1625
EcoO65I GGTNACC 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 1321
EcoRII CCWGG 6 cut(s) 477, 529, 757, 1599, 1900, 2259
EcoRV GATATC 1 cut(s) 2053
EcoT14I CCWWGG 3 cut(s) 156, 1204, 2032
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 2 cut(s) 17, 2350
ErhI CCWWGG 3 cut(s) 156, 1204, 2032
Esp3I CGTCTC 1 cut(s) 1996
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 2 cut(s) 1012, 1436
FauI CCCGC 2 cut(s) 351, 2231
Fnu4HI GCNGC 7 cut(s) 1230, 1248, 1782, 2193, 2282, 2376, 2379
FriOI GRGCYC 2 cut(s) 17, 2350
Fsp4HI GCNGC 7 cut(s) 1230, 1248, 1782, 2193, 2282, 2376, 2379
FspI TGCGCA 1 cut(s) 2022
GlaI GCGC 1 cut(s) 2022
GluI GCNGC 7 cut(s) 1230, 1248, 1782, 2193, 2282, 2376, 2379
GsaI CCCAGC 2 cut(s) 1889, 2375
GsuI CTGGAG 3 cut(s) 825, 1785, 2334
HaeIII GGCC 8 cut(s) 454, 832, 1066, 1549, 1626, 1870, 1900, 2369
HapII CCGG 4 cut(s) 451, 1133, 1553, 2311
HgaI GACGC 1 cut(s) 1433
HhaI GCGC 1 cut(s) 2023
Hin1I GRCGYC 1 cut(s) 1906
Hin6I GCGC 1 cut(s) 2021
HinP1I GCGC 1 cut(s) 2021
HincII GTYRAC 1 cut(s) 2005
HindII GTYRAC 1 cut(s) 2005
HindIII AAGCTT 1 cut(s) 1944
HinfI GANTC 8 cut(s) 443, 938, 964, 1164, 1733, 1791, 2207, 2275
HpaII CCGG 4 cut(s) 451, 1133, 1553, 2311
HphI GGTGA 7 cut(s) 194, 479, 545, 869, 983, 1512, 1977
Hpy166II GTNNAC 4 cut(s) 364, 1034, 1130, 2005
Hpy188I TCNGA 9 cut(s) 12, 269, 663, 1005, 1089, 1959, 2093, 2274, 2305
Hpy8I GTNNAC 4 cut(s) 364, 1034, 1130, 2005
HpyCH4III ACNGT 6 cut(s) 190, 223, 542, 1396, 1471, 1843
HpyCH4IV ACGT 3 cut(s) 592, 1668, 1906
HpyF10VI GCNNNNNNNGC 6 cut(s) 1226, 1364, 1623, 1778, 2018, 2375
HpySE526I ACGT 3 cut(s) 592, 1668, 1906
Hsp92I GRCGYC 1 cut(s) 1906
HspAI GCGC 1 cut(s) 2021
KpnI GGTACC 1 cut(s) 1409
Kzo9I GATC 4 cut(s) 7, 1222, 2300, 2392
LguI GCTCTTC 2 cut(s) 710, 1257
Lsp1109I GCAGC 6 cut(s) 1234, 1768, 2204, 2293, 2362, 2365
LweI GCATC 9 cut(s) 406, 483, 1269, 1297, 1498, 1626, 1759, 2032, 2230
MaeII ACGT 3 cut(s) 592, 1668, 1906
MaeIII GTNAC 8 cut(s) 182, 398, 467, 681, 1333, 1518, 1632, 1983
MalI GATC 4 cut(s) 9, 1224, 2302, 2394
MbiI CCGCTC 1 cut(s) 1232
MboI GATC 4 cut(s) 7, 1222, 2300, 2392
MfeI CAATTG 2 cut(s) 387, 1584
MflI RGATCY 2 cut(s) 7, 2300
MhlI GDGCHC 4 cut(s) 17, 1036, 2330, 2350
MlyI GAGTC 4 cut(s) 958, 1727, 1800, 2269
MmeI TCCRAC 3 cut(s) 616, 1123, 2328
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 2 cut(s) 165, 1759
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 3 cut(s) 719, 1209, 2031
MspA1I CMGCKG 2 cut(s) 2375, 2403
MspI CCGG 4 cut(s) 451, 1133, 1553, 2311
MspR9I CCNGG 7 cut(s) 479, 531, 759, 1554, 1601, 1902, 2261
MunI CAATTG 2 cut(s) 387, 1584
Mva1269I GAATGC 4 cut(s) 809, 910, 1601, 2264
MvaI CCWGG 6 cut(s) 479, 531, 759, 1601, 1902, 2261
MvnI CGCG 1 cut(s) 669
MwoI GCNNNNNNNGC 6 cut(s) 1226, 1364, 1623, 1778, 2018, 2375
NciI CCSGG 1 cut(s) 1554
NcoI CCATGG 3 cut(s) 156, 1204, 2032
NdeII GATC 4 cut(s) 7, 1222, 2300, 2392
NlaIV GGNNCC 8 cut(s) 9, 1407, 1627, 2178, 2302, 2315, 2329, 2370
NmuCI GTSAC 5 cut(s) 182, 467, 1333, 1518, 1983
NsbI TGCGCA 1 cut(s) 2022
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 3 cut(s) 1040, 1519, 1974
PceI AGGCCT 1 cut(s) 1900
PciI ACATGT 1 cut(s) 1036
PciSI GCTCTTC 2 cut(s) 710, 1257
PctI GAATGC 4 cut(s) 809, 910, 1601, 2264
PdmI GAANNNNTTC 2 cut(s) 165, 1759
PfeI GAWTC 4 cut(s) 443, 938, 1164, 2207
PflFI GACNNNGTC 1 cut(s) 1445
PkrI GCNGC 7 cut(s) 1231, 1249, 1783, 2194, 2283, 2377, 2380
PleI GAGTC 4 cut(s) 958, 1727, 1799, 2269
PpsI GAGTC 4 cut(s) 958, 1727, 1799, 2269
PscI ACATGT 1 cut(s) 1036
PshBI ATTAAT 1 cut(s) 825
Psp124BI GAGCTC 2 cut(s) 17, 2350
Psp6I CCWGG 6 cut(s) 477, 529, 757, 1599, 1900, 2259
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 2 cut(s) 1885, 2371
PspGI CCWGG 6 cut(s) 477, 529, 757, 1599, 1900, 2259
PspN4I GGNNCC 8 cut(s) 9, 1407, 1627, 2178, 2302, 2315, 2329, 2370
PspPI GGNCC 5 cut(s) 752, 830, 1547, 1625, 2368
PstI CTGCAG 4 cut(s) 1786, 2283, 2338, 2383
PstNI CAGNNNCTG 3 cut(s) 530, 2278, 2378
PsuI RGATCY 2 cut(s) 7, 2300
PsyI GACNNNGTC 1 cut(s) 1445
PvuII CAGCTG 2 cut(s) 2375, 2403
RsaI GTAC 5 cut(s) 1131, 1240, 1407, 2258, 2399
RsaNI GTAC 5 cut(s) 1130, 1239, 1406, 2257, 2398
RseI CAYNNNNRTG 3 cut(s) 719, 1209, 2031
SacI GAGCTC 2 cut(s) 17, 2350
SapI GCTCTTC 2 cut(s) 710, 1257
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 7 cut(s) 1230, 1248, 1782, 2193, 2282, 2376, 2379
Sau3AI GATC 4 cut(s) 7, 1222, 2300, 2392
Sau96I GGNCC 5 cut(s) 752, 830, 1547, 1625, 2368
SchI GAGTC 4 cut(s) 958, 1727, 1800, 2269
ScrFI CCNGG 7 cut(s) 479, 531, 759, 1554, 1601, 1902, 2261
SduI GDGCHC 4 cut(s) 17, 1036, 2330, 2350
SfaNI GCATC 9 cut(s) 406, 483, 1269, 1297, 1498, 1626, 1759, 2032, 2230
SfcI CTRYAG 5 cut(s) 789, 1782, 2279, 2334, 2379
SinI GGWCC 1 cut(s) 752
SmiMI CAYNNNNRTG 3 cut(s) 719, 1209, 2031
SmlI CTYRAG 2 cut(s) 874, 1022
SmoI CTYRAG 2 cut(s) 874, 1022
SseBI AGGCCT 1 cut(s) 1900
SsiI CCGC 5 cut(s) 5, 358, 1230, 1978, 2224
SspI AATATT 1 cut(s) 2101
SstI GAGCTC 2 cut(s) 17, 2350
StuI AGGCCT 1 cut(s) 1900
StyD4I CCNGG 7 cut(s) 477, 529, 757, 1552, 1599, 1900, 2259
StyI CCWWGG 3 cut(s) 156, 1204, 2032
TaaI ACNGT 6 cut(s) 190, 223, 542, 1396, 1471, 1843
TaiI ACGT 3 cut(s) 595, 1671, 1909
TaqI TCGA 4 cut(s) 94, 378, 1736, 2202
TatI WGTACW 2 cut(s) 1238, 2397
TauI GCSGC 1 cut(s) 1232
TfiI GAWTC 4 cut(s) 443, 938, 1164, 2207
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 6 cut(s) 67, 193, 1262, 1488, 2062, 2338
TseFI GTSAC 5 cut(s) 182, 467, 1333, 1518, 1983
TseI GCWGC 6 cut(s) 1247, 1781, 2192, 2281, 2375, 2378
Tsp45I GTSAC 5 cut(s) 182, 467, 1333, 1518, 1983
TspGWI ACGGA 3 cut(s) 417, 1253, 1661
TspRI CASTG 6 cut(s) 67, 193, 1262, 1488, 2062, 2338
Tth111I GACNNNGTC 1 cut(s) 1445
VneI GTGCAC 1 cut(s) 1032
VpaK11BI GGWCC 1 cut(s) 752
VspI ATTAAT 1 cut(s) 825
XagI CCTNNNNNAGG 1 cut(s) 1527
XapI RAATTY 5 cut(s) 1321, 1508, 1606, 1756, 2216
XceI RCATGY 3 cut(s) 1040, 1519, 1974
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 2039
XmnI GAANNNNTTC 2 cut(s) 165, 1759
ZraI GACGTC 1 cut(s) 1907
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.