MD14G1175800.v1.1

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013824
Physical Location & Seq
Reverse (-)
26914441 .. 26917862
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1175800.v1.1.491

Sequence Viewer

Length: 2478 bp
ATGAGAAGATTTGATTATTTGTTGGTTGTGATGAGTACTTCGTTACGAGAAACTAAAGTTTTTATTTTCAAATACAGGAAAAGCAACAAGAAAGATTTTTCGACTGCTATTTTGGAACGAAAGAAGTCTCCTAATCGACTTGTTGTCGATGAAGCTGTCAATGATGATAATTCTGTGATTTCCTTGCACCCTGCTGCAATGGAGAAGCTTCAGTTTTTCCGTGGAGACACTGTTTTAATTAAGGGCAAGAAAAGGAGAGACACAATTTGCATTATTATTGCCGATGATGAATGCGAGGAACCGAAAATCAGAATGAACAAAGTTGTGAGAGCTAATCTTAGGGTTCGCCTTGGAGATGTTGTGTCTGTTCATCCCTGCCCTTATGTCAAGTATGGAAAGAAGGTTCACATCCTTCCAATCGATGATACAATTGAAGGAGTGACAGGAAGCTTATTCGATGCATATTTGAAGCCATACTTCGTTGATTCTTACCGGCCTACGAGGAAGGGTGATCTTTTCCTGGTTCGGGGCATGATGCGAAGCGTTGAGTTCAAGGTCATGGAGACAGACCCTGCTGAGTATTGCATTGTTGCACCGGATACTGAGATTTTCTGTGAGGGAGAGCCTGTCAAACGTGAGGACGAGGAAAGACTAGAGGATGTGGGATATGATGATGTTGGAGGTGTTAGAAAGCAAATGGCCCAGATACGGGAGTTAGTAGAACTGCCTCTTAGACATCCGCAGCTCTTCAAATCGATTGGAGTTAAACCCCCGAAGGGCATTTTACTCTATGGGCCACCCGGGACTGGAAAAACTTTGATTGCAAGGGCTGTAGCGAATGAGACTGGAGTGTTCTTTGTTTTGATCAATGGACCTGAAATAATGTCCAAGATGGCAGGTGAAAGTGAAAGCAACTTGAGGAAGGTGTTTGAGGAAGCCGAAAAGAATGCACCCTCAATCATTTTTATTGATGAGATAGATTCTATTGCTCCAAAGAGAGAAAAGACCCACGGTGAAGTGGAAAGGCGTATAGTTTCCCAGCTCCTGACTTTAATGGACGGCCTTAAGTCCCGTGCGCATGTCATTGTCATGGGAGCAACGAATAGGCCAAACAGCATCGACCCTGCTCTGAGGAGATTTGGAAGATTCGATAGAGAGATTGACATTGGCGTGCCGGATGAAGTTGGAAGATTGGAAGTTTTACGAATCCATACAAAGAGGATGAAACTTGCAGATGATGTTGATCTCGAAAGAGTTGCAAAAGATACCCATGGTTTTGTTGGTGCAGATCTTGCTGCTCTCTGCACAGAGGCCGCACTTCAGTGCATCCGCGAAAAAATGGATGTTATTGATTTGGAAGACGAAACGATTGATGCTGAGGTGTTGAATTCGATGGCTGTGACGAATGAACACTTTCAGACCTCATTGTCCTCTACAAATCCTTCTGCCTTGCGCGAAACTGTTGTGGAGGTACCAAATGTTTCGTGGGATGCCATTGGTGGGTTGGACAACGTCAAAAGAGAACTTCAAGAGACTGTTTCAATCCCCGTGGAGCATCCAGAGATGTTTGAGAAATTTGGCATGTCGCCTTCTAAAGGCGTTCTCTTTTATGGACCGCCTGGCTGTGGGAAAACCCTTCTCGCCAAGGCAATTGCCAGCGAATGTCAGGCCAACTTCATCAGTATCAAAGGACCCGAGTTGCTAACAATGTGGTTTGGAGAAAGTGAGGCCAATGTGCGCGAGATATTTGACAAGGCTCGCCAATCTGCTCCTTGTGTTCTCTTCTTTGATGAGCTAGACTCGATTGCAACTCAGCGTGGAAATTCTGTGGGAGATGCCGGTGGTGCTGGAGACAGAGTCTTGAACCAACTTCTAACTGAAATGGATGGAATGAATGCAAAGAAAACCGTCTTTATCATTGGGGCAACAAACAGGCCAGATATTATTGACCCGGCATTGCTCAGGCCTGGACGTCTTGACCAACTTATCTACATCCCACTCCCAGATGAGGCTTCGCGCCTTCAGATTTTTAAAGCATGTTTGCGCAAGTCCCCCATCTCTAAGGATATTGACCTAGCAGCTCTTGCCCGTTATACGCACGGTTTTAGTGGTGCAGATATCACTGAGATATGTCAACGTGCCTGCAAATATGCCATCAGAGAAGACATTGAGAAGGATATTGAGAGGGAAAGAAGAAAGAATGCTAACCCCGAAGCCATGGAAGAAGATGAAATTGATGAGGTAGCCGAGATCAAGCCAGCACACTTTGAGGAATCGATGAAATATGCCCGAAGAAGTGTGAGTGATGCGGACATCAAGAAGTACCAGCTATTTGCTCAGACTTTGCAGCAATCTCGTGGGTTTGGATCCGAATTTCGATTTCCTGCTCAACAGACAGAGAACAATGCAGGAGAAGCTGCTCTATCCCCATTTTCTTCAGCCGCGGTTGCTGGAGAGGAAGCTGATCTCTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

826

Amino Acids

92.31

Weight (kDa)

5.35

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 46 - 127 2.2e-22 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 148 - 211 4.2e-10 Cell division protein 48 (CDC48), domain 2
RuvB_N PF05496 260 - 335 9.2e-07 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 261 - 390 7e-49 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 413 - 454 2.8e-16 AAA+ lid domain
AAA PF00004 534 - 667 1.3e-46 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 689 - 728 4.5e-13 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 887
AasI GACNNNNNNGTC 1 cut(s) 1426
AatII GACGTC 1 cut(s) 1975
Acc16I TGCGCA 2 cut(s) 1077, 2045
Acc36I ACCTGC 1 cut(s) 887
Acc65I GGTACC 1 cut(s) 1471
AccB1I GGYRCC 1 cut(s) 1471
AccII CGCG 5 cut(s) 1332, 1455, 1740, 2017, 2444
AciI CCGC 7 cut(s) 740, 1314, 1330, 1616, 2309, 2442, 2444
AclWI GGATC 2 cut(s) 2361, 2374
AcsI RAATTY 4 cut(s) 1387, 1574, 1822, 2372
AcuI CTGAAG 4 cut(s) 194, 1304, 2006, 2421
AcyI GRCGYC 1 cut(s) 1972
AfaI GTAC 3 cut(s) 37, 1473, 2324
AfiI CCNNNNNNNGG 9 cut(s) 526, 708, 709, 776, 806, 1500, 1595, 1625, 2008
AflII CTTAAG 1 cut(s) 1064
AgsI TTSAA 9 cut(s) 70, 434, 469, 553, 751, 1387, 1529, 1542, 1864
AhdI GACNNNNNGTC 1 cut(s) 143
AjnI CCWGG 3 cut(s) 519, 1618, 1966
AleI CACNNNNGTG 1 cut(s) 1321
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
Alw26I GTCTC 7 cut(s) 132, 219, 252, 557, 836, 1526, 1845
AlwI GGATC 2 cut(s) 2361, 2374
AlwNI CAGNNNCTG 2 cut(s) 572, 1045
Ama87I CYCGRG 2 cut(s) 800, 1694
ApeKI GCWGC 6 cut(s) 194, 742, 1295, 2078, 2347, 2417
ApoI RAATTY 4 cut(s) 1387, 1574, 1822, 2372
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
Asp700I GAANNNNTTC 1 cut(s) 1413
Asp718I GGTACC 1 cut(s) 1471
AspLEI GCGC 5 cut(s) 1078, 1455, 1740, 2019, 2046
AspS9I GGNCC 5 cut(s) 700, 794, 872, 1613, 1691
AsuC2I CCSGG 3 cut(s) 801, 802, 1952
AsuHPI GGTGA 3 cut(s) 521, 911, 1025
AvaI CYCGRG 2 cut(s) 800, 1694
AvaII GGWCC 3 cut(s) 872, 1613, 1691
BaeI ACNNNNGTAYC 2 cut(s) 698, 731
BamHI GGATCC 1 cut(s) 2366
BanI GGYRCC 1 cut(s) 1471
BauI CACGAG 1 cut(s) 2355
BbsI GAAGAC 2 cut(s) 1365, 2169
BbvCI CCTCAGC 1 cut(s) 1377
BbvI GCAGC 6 cut(s) 181, 754, 1282, 2090, 2359, 2404
BccI CCATC 5 cut(s) 886, 1387, 1880, 2063, 2162
BceAI ACGGC 1 cut(s) 1075
BcgI CGANNNNNNTGC 6 cut(s) 929, 963, 1238, 1272, 2336, 2370
BciT130I CCWGG 3 cut(s) 521, 1620, 1968
BciVI GTATCC 1 cut(s) 592
BclI TGATCA 1 cut(s) 864
BcnI CCSGG 3 cut(s) 801, 802, 1952
BcoDI GTCTC 7 cut(s) 132, 219, 252, 557, 836, 1526, 1845
BfaI CTAG 3 cut(s) 653, 1796, 2075
BfmI CTRYAG 1 cut(s) 831
BfrI CTTAAG 1 cut(s) 1064
BfuAI ACCTGC 1 cut(s) 887
BfuI GTATCC 1 cut(s) 592
BglII AGATCT 1 cut(s) 1288
BisI GCNGC 8 cut(s) 195, 743, 1296, 1314, 2079, 2348, 2418, 2442
BlsI GCNGC 8 cut(s) 196, 744, 1297, 1315, 2080, 2349, 2419, 2443
BmcAI AGTACT 1 cut(s) 37
Bme1390I CCNGG 6 cut(s) 521, 801, 802, 1620, 1952, 1968
Bme18I GGWCC 3 cut(s) 872, 1613, 1691
BmeRI GACNNNNNGTC 1 cut(s) 143
BmeT110I CYCGRG 2 cut(s) 800, 1694
BmgT120I GGNCC 5 cut(s) 700, 794, 872, 1613, 1691
BmiI GGNNCC 4 cut(s) 300, 1473, 1693, 2368
BmrFI CCNGG 6 cut(s) 521, 801, 802, 1620, 1952, 1968
BmsI GCATC 9 cut(s) 448, 525, 1125, 1335, 1363, 1480, 1564, 1825, 2296
BpiI GAAGAC 2 cut(s) 1365, 2169
BplI GAGNNNNNCTC 2 cut(s) 1784, 1816
BpmI CTGGAG 3 cut(s) 867, 1869, 2472
Bpu10I CCTNAGC 2 cut(s) 1377, 1961
BpuEI CTTGAG 1 cut(s) 937
BpuMI CCSGG 3 cut(s) 801, 802, 1952
Bsa29I ATCGAT 3 cut(s) 420, 755, 2276
BsaBI GATNNNNATC 1 cut(s) 1242
BsaHI GRCGYC 1 cut(s) 1972
BsaJI CCNNGG 9 cut(s) 220, 349, 800, 1009, 1270, 1548, 1644, 2217, 2442
BsaWI WCCGGW 1 cut(s) 595
BsaXI ACNNNNNCTCC 8 cut(s) 216, 246, 345, 375, 612, 642, 1842, 1872
Bsc4I CCNNNNNNNGG 9 cut(s) 526, 708, 709, 776, 806, 1500, 1595, 1625, 2008
Bse118I RCCGGY 2 cut(s) 492, 1838
Bse1I ACTGG 2 cut(s) 811, 850
Bse3DI GCAATG 2 cut(s) 204, 1955
Bse8I GATNNNNATC 1 cut(s) 1242
BseBI CCWGG 3 cut(s) 521, 1620, 1968
BseCI ATCGAT 3 cut(s) 420, 755, 2276
BseDI CCNNGG 9 cut(s) 220, 349, 800, 1009, 1270, 1548, 1644, 2217, 2442
BseJI GATNNNNATC 1 cut(s) 1242
BseLI CCNNNNNNNGG 9 cut(s) 526, 708, 709, 776, 806, 1500, 1595, 1625, 2008
BseMI GCAATG 2 cut(s) 204, 1955
BseMII CTCAG 8 cut(s) 567, 594, 1121, 1368, 1826, 1975, 2115, 2351
BseNI ACTGG 2 cut(s) 811, 850
BseRI GAGGAG 1 cut(s) 1147
BseXI GCAGC 6 cut(s) 181, 754, 1282, 2090, 2359, 2404
BseYI CCCAGC 1 cut(s) 1038
BsgI GTGCAG 3 cut(s) 1288, 1305, 2133
Bsh1236I CGCG 5 cut(s) 1332, 1455, 1740, 2017, 2444
BshNI GGYRCC 1 cut(s) 1471
BshVI ATCGAT 3 cut(s) 420, 755, 2276
BsiHKCI CYCGRG 2 cut(s) 800, 1694
BsiSI CCGG 6 cut(s) 493, 596, 801, 1175, 1839, 1952
BslFI GGGAC 3 cut(s) 817, 1054, 2035
BslI CCNNNNNNNGG 9 cut(s) 526, 708, 709, 776, 806, 1500, 1595, 1625, 2008
BsmAI GTCTC 7 cut(s) 132, 219, 252, 557, 836, 1526, 1845
BsmFI GGGAC 3 cut(s) 817, 1054, 2035
BsmI GAATGC 4 cut(s) 296, 952, 1900, 2206
BsoBI CYCGRG 2 cut(s) 800, 1694
Bsp143I GATC 7 cut(s) 511, 864, 1243, 1288, 2250, 2366, 2464
Bsp19I CCATGG 2 cut(s) 1270, 2217
BspACI CCGC 7 cut(s) 740, 1314, 1330, 1616, 2309, 2442, 2444
BspCNI CTCAG 8 cut(s) 568, 595, 1122, 1369, 1825, 1974, 2116, 2350
BspDI ATCGAT 3 cut(s) 420, 755, 2276
BspFNI CGCG 5 cut(s) 1332, 1455, 1740, 2017, 2444
BspLI GGNNCC 4 cut(s) 300, 1473, 1693, 2368
BspMI ACCTGC 1 cut(s) 887
BspPI GGATC 2 cut(s) 2361, 2374
BspQI GCTCTTC 1 cut(s) 752
BspT107I GGYRCC 1 cut(s) 1471
BspTI CTTAAG 1 cut(s) 1064
BsrDI GCAATG 2 cut(s) 204, 1955
BsrFI RCCGGY 2 cut(s) 492, 1838
BsrI ACTGG 2 cut(s) 811, 850
BssAI RCCGGY 2 cut(s) 492, 1838
BssECI CCNNGG 9 cut(s) 220, 349, 800, 1009, 1270, 1548, 1644, 2217, 2442
BssMI GATC 7 cut(s) 511, 864, 1243, 1288, 2250, 2366, 2464
BssNI GRCGYC 1 cut(s) 1972
BssSI CACGAG 1 cut(s) 2355
BssT1I CCWWGG 4 cut(s) 349, 1270, 1644, 2217
Bst2BI CACGAG 1 cut(s) 2355
Bst2UI CCWGG 3 cut(s) 521, 1620, 1968
Bst4CI ACNGT 6 cut(s) 232, 1013, 1462, 1537, 1909, 2102
Bst6I CTCTTC 2 cut(s) 752, 1787
BstACI GRCGYC 1 cut(s) 1972
BstAFI CTTAAG 1 cut(s) 1064
BstAPI GCANNNNNTGC 2 cut(s) 1292, 2084
BstC8I GCNNGC 5 cut(s) 1172, 1657, 1759, 2143, 2259
BstDSI CCRYGG 6 cut(s) 220, 1009, 1270, 1548, 2217, 2442
BstENI CCTNNNNNAGG 1 cut(s) 1593
BstFNI CGCG 5 cut(s) 1332, 1455, 1740, 2017, 2444
BstHHI GCGC 5 cut(s) 1078, 1455, 1740, 2019, 2046
BstKTI GATC 7 cut(s) 514, 867, 1246, 1291, 2253, 2369, 2467
BstMAI GTCTC 7 cut(s) 132, 219, 252, 557, 836, 1526, 1845
BstMBI GATC 7 cut(s) 511, 864, 1243, 1288, 2250, 2366, 2464
BstMWI GCNNNNNNNGC 5 cut(s) 1292, 1844, 2084, 2414, 2447
BstNI CCWGG 3 cut(s) 521, 1620, 1968
BstNSI RCATGY 3 cut(s) 1082, 1585, 2040
BstSCI CCNGG 6 cut(s) 519, 799, 800, 1618, 1950, 1966
BstSFI CTRYAG 1 cut(s) 831
BstUI CGCG 5 cut(s) 1332, 1455, 1740, 2017, 2444
BstV1I GCAGC 6 cut(s) 181, 754, 1282, 2090, 2359, 2404
BstV2I GAAGAC 2 cut(s) 1365, 2169
BstX2I RGATCY 2 cut(s) 1288, 2366
BstYI RGATCY 2 cut(s) 1288, 2366
Bsu15I ATCGAT 3 cut(s) 420, 755, 2276
BsuI GTATCC 1 cut(s) 592
BsuTUI ATCGAT 3 cut(s) 420, 755, 2276
BtgI CCRYGG 6 cut(s) 220, 1009, 1270, 1548, 2217, 2442
BtsIMutI CAGTG 3 cut(s) 228, 1328, 2121
BveI ACCTGC 1 cut(s) 887
Cac8I GCNNGC 5 cut(s) 1172, 1657, 1759, 2143, 2259
CaiI CAGNNNCTG 2 cut(s) 572, 1045
CfoI GCGC 5 cut(s) 1078, 1455, 1740, 2019, 2046
Cfr10I RCCGGY 2 cut(s) 492, 1838
Cfr13I GGNCC 5 cut(s) 700, 794, 872, 1613, 1691
Cfr42I CCGCGG 1 cut(s) 2445
Cfr9I CCCGGG 1 cut(s) 800
ClaI ATCGAT 3 cut(s) 420, 755, 2276
Csp6I GTAC 3 cut(s) 36, 1472, 2323
CspCI CAANNNNNGTGG 2 cut(s) 1530, 1565
CviAII CATG 8 cut(s) 532, 559, 1079, 1090, 1271, 1582, 2037, 2218
CviQI GTAC 3 cut(s) 36, 1472, 2323
DpnI GATC 7 cut(s) 513, 866, 1245, 1290, 2252, 2368, 2466
DpnII GATC 7 cut(s) 511, 864, 1243, 1288, 2250, 2366, 2464
DraI TTTAAA 1 cut(s) 2032
DrdI GACNNNNNNGTC 1 cut(s) 1426
DriI GACNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 1426
Eam1104I CTCTTC 2 cut(s) 752, 1787
Eam1105I GACNNNNNGTC 1 cut(s) 143
EarI CTCTTC 2 cut(s) 752, 1787
Eco130I CCWWGG 4 cut(s) 349, 1270, 1644, 2217
Eco147I AGGCCT 1 cut(s) 1966
Eco32I GATATC 1 cut(s) 2119
Eco47I GGWCC 3 cut(s) 872, 1613, 1691
Eco57I CTGAAG 4 cut(s) 194, 1304, 2006, 2421
Eco88I CYCGRG 2 cut(s) 800, 1694
EcoNI CCTNNNNNAGG 1 cut(s) 1593
EcoO109I RGGNCCY 1 cut(s) 1691
EcoRI GAATTC 1 cut(s) 1387
EcoRII CCWGG 3 cut(s) 519, 1618, 1966
EcoRV GATATC 1 cut(s) 2119
EcoT14I CCWWGG 4 cut(s) 349, 1270, 1644, 2217
EcoT22I ATGCAT 1 cut(s) 463
ErhI CCWWGG 4 cut(s) 349, 1270, 1644, 2217
FaeI CATG 8 cut(s) 535, 562, 1082, 1093, 1274, 1585, 2040, 2221
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 3 cut(s) 817, 1054, 2035
FatI CATG 8 cut(s) 531, 558, 1078, 1089, 1270, 1581, 2036, 2217
FbaI TGATCA 1 cut(s) 864
Fnu4HI GCNGC 8 cut(s) 195, 743, 1296, 1314, 2079, 2348, 2418, 2442
Fsp4HI GCNGC 8 cut(s) 195, 743, 1296, 1314, 2079, 2348, 2418, 2442
FspAI RTGCGCAY 1 cut(s) 1077
FspBI CTAG 3 cut(s) 653, 1796, 2075
FspI TGCGCA 2 cut(s) 1077, 2045
GlaI GCGC 5 cut(s) 1077, 1454, 1739, 2018, 2045
GluI GCNGC 8 cut(s) 195, 743, 1296, 1314, 2079, 2348, 2418, 2442
GsaI CCCAGC 1 cut(s) 1042
GsuI CTGGAG 3 cut(s) 867, 1869, 2472
HapII CCGG 6 cut(s) 493, 596, 801, 1175, 1839, 1952
HhaI GCGC 5 cut(s) 1078, 1455, 1740, 2019, 2046
Hin1I GRCGYC 1 cut(s) 1972
Hin1II CATG 8 cut(s) 535, 562, 1082, 1093, 1274, 1585, 2040, 2221
Hin6I GCGC 5 cut(s) 1076, 1453, 1738, 2017, 2044
HinP1I GCGC 5 cut(s) 1076, 1453, 1738, 2017, 2044
HincII GTYRAC 1 cut(s) 2135
HindII GTYRAC 1 cut(s) 2135
HindIII AAGCTT 2 cut(s) 206, 448
HinfI GANTC 7 cut(s) 485, 980, 1146, 1206, 1799, 1857, 2273
HpaII CCGG 6 cut(s) 493, 596, 801, 1175, 1839, 1952
HphI GGTGA 3 cut(s) 521, 911, 1025
Hpy166II GTNNAC 2 cut(s) 406, 2135
Hpy188I TCNGA 7 cut(s) 311, 1131, 1419, 2025, 2159, 2340, 2371
Hpy188III TCNNGA 7 cut(s) 1045, 1247, 1529, 1559, 1861, 1976, 2317
Hpy8I GTNNAC 2 cut(s) 406, 2135
HpyCH4III ACNGT 6 cut(s) 232, 1013, 1462, 1537, 1909, 2102
HpyCH4IV ACGT 4 cut(s) 634, 1512, 1972, 2137
HpyF10VI GCNNNNNNNGC 5 cut(s) 1292, 1844, 2084, 2414, 2447
HpySE526I ACGT 4 cut(s) 634, 1512, 1972, 2137
Hsp92I GRCGYC 1 cut(s) 1972
Hsp92II CATG 8 cut(s) 535, 562, 1082, 1093, 1274, 1585, 2040, 2221
HspAI GCGC 5 cut(s) 1076, 1453, 1738, 2017, 2044
KpnI GGTACC 1 cut(s) 1475
Ksp22I TGATCA 1 cut(s) 864
KspI CCGCGG 1 cut(s) 2445
Kzo9I GATC 7 cut(s) 511, 864, 1243, 1288, 2250, 2366, 2464
LguI GCTCTTC 1 cut(s) 752
LmnI GCTCC 5 cut(s) 994, 1047, 1094, 1552, 1774
Lsp1109I GCAGC 6 cut(s) 181, 754, 1282, 2090, 2359, 2404
LweI GCATC 9 cut(s) 448, 525, 1125, 1335, 1363, 1480, 1564, 1825, 2296
MaeI CTAG 3 cut(s) 653, 1796, 2075
MaeII ACGT 4 cut(s) 634, 1512, 1972, 2137
MaeIII GTNAC 3 cut(s) 42, 439, 1399
MalI GATC 7 cut(s) 513, 866, 1245, 1290, 2252, 2368, 2466
MboI GATC 7 cut(s) 511, 864, 1243, 1288, 2250, 2366, 2464
MfeI CAATTG 2 cut(s) 429, 1650
MflI RGATCY 2 cut(s) 1288, 2366
MlyI GAGTC 2 cut(s) 1793, 1866
MmeI TCCRAC 3 cut(s) 658, 1165, 1485
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 1413
MseI TTAA 6 cut(s) 236, 240, 765, 1052, 1065, 2031
MslI CAYNNNNRTG 3 cut(s) 1088, 1169, 1321
MspA1I CMGCKG 1 cut(s) 2444
MspCI CTTAAG 1 cut(s) 1064
MspI CCGG 6 cut(s) 493, 596, 801, 1175, 1839, 1952
MspR9I CCNGG 6 cut(s) 521, 801, 802, 1620, 1952, 1968
MunI CAATTG 2 cut(s) 429, 1650
Mva1269I GAATGC 4 cut(s) 296, 952, 1900, 2206
MvaI CCWGG 3 cut(s) 521, 1620, 1968
MvnI CGCG 5 cut(s) 1332, 1455, 1740, 2017, 2444
MwoI GCNNNNNNNGC 5 cut(s) 1292, 1844, 2084, 2414, 2447
NciI CCSGG 3 cut(s) 801, 802, 1952
NcoI CCATGG 2 cut(s) 1270, 2217
NdeII GATC 7 cut(s) 511, 864, 1243, 1288, 2250, 2366, 2464
NlaIII CATG 8 cut(s) 535, 562, 1082, 1093, 1274, 1585, 2040, 2221
NlaIV GGNNCC 4 cut(s) 300, 1473, 1693, 2368
NmeAIII GCCGAG 1 cut(s) 2272
NmuCI GTSAC 2 cut(s) 439, 1399
NsbI TGCGCA 2 cut(s) 1077, 2045
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 3 cut(s) 1082, 1585, 2040
OliI CACNNNNGTG 1 cut(s) 1321
PacI TTAATTAA 1 cut(s) 240
PaqCI CACCTGC 1 cut(s) 887
PceI AGGCCT 1 cut(s) 1966
PciSI GCTCTTC 1 cut(s) 752
PctI GAATGC 4 cut(s) 296, 952, 1900, 2206
PdmI GAANNNNTTC 1 cut(s) 1413
PfeI GAWTC 5 cut(s) 485, 980, 1146, 1206, 2273
PflFI GACNNNGTC 2 cut(s) 1511, 1856
PkrI GCNGC 8 cut(s) 196, 744, 1297, 1315, 2080, 2349, 2419, 2443
PleI GAGTC 2 cut(s) 1793, 1865
PpsI GAGTC 2 cut(s) 1793, 1865
PpuMI RGGWCCY 1 cut(s) 1691
Psp5II RGGWCCY 1 cut(s) 1691
Psp6I CCWGG 3 cut(s) 519, 1618, 1966
PspFI CCCAGC 1 cut(s) 1038
PspGI CCWGG 3 cut(s) 519, 1618, 1966
PspN4I GGNNCC 4 cut(s) 300, 1473, 1693, 2368
PspPI GGNCC 5 cut(s) 700, 794, 872, 1613, 1691
PspPPI RGGWCCY 1 cut(s) 1691
PstNI CAGNNNCTG 2 cut(s) 572, 1045
PsuI RGATCY 2 cut(s) 1288, 2366
PsyI GACNNNGTC 2 cut(s) 1511, 1856
RsaI GTAC 3 cut(s) 37, 1473, 2324
RsaNI GTAC 3 cut(s) 36, 1472, 2323
RseI CAYNNNNRTG 3 cut(s) 1088, 1169, 1321
SacII CCGCGG 1 cut(s) 2445
SapI GCTCTTC 1 cut(s) 752
SaqAI TTAA 6 cut(s) 236, 240, 765, 1052, 1065, 2031
SatI GCNGC 8 cut(s) 195, 743, 1296, 1314, 2079, 2348, 2418, 2442
Sau3AI GATC 7 cut(s) 511, 864, 1243, 1288, 2250, 2366, 2464
Sau96I GGNCC 5 cut(s) 700, 794, 872, 1613, 1691
ScaI AGTACT 1 cut(s) 37
SchI GAGTC 2 cut(s) 1793, 1866
ScrFI CCNGG 6 cut(s) 521, 801, 802, 1620, 1952, 1968
SfaNI GCATC 9 cut(s) 448, 525, 1125, 1335, 1363, 1480, 1564, 1825, 2296
SfcI CTRYAG 1 cut(s) 831
Sfr303I CCGCGG 1 cut(s) 2445
SgrBI CCGCGG 1 cut(s) 2445
SinI GGWCC 3 cut(s) 872, 1613, 1691
SmaI CCCGGG 1 cut(s) 802
SmiMI CAYNNNNRTG 3 cut(s) 1088, 1169, 1321
SmlI CTYRAG 2 cut(s) 916, 1064
SmoI CTYRAG 2 cut(s) 916, 1064
SseBI AGGCCT 1 cut(s) 1966
SsiI CCGC 7 cut(s) 740, 1314, 1330, 1616, 2309, 2442, 2444
SspMI CTAG 3 cut(s) 653, 1796, 2075
StuI AGGCCT 1 cut(s) 1966
StyD4I CCNGG 6 cut(s) 519, 799, 800, 1618, 1950, 1966
StyI CCWWGG 4 cut(s) 349, 1270, 1644, 2217
TaaI ACNGT 6 cut(s) 232, 1013, 1462, 1537, 1909, 2102
TaiI ACGT 4 cut(s) 637, 1515, 1975, 2140
TatI WGTACW 1 cut(s) 35
TauI GCSGC 2 cut(s) 1316, 2444
TfiI GAWTC 5 cut(s) 485, 980, 1146, 1206, 2273
Tru1I TTAA 6 cut(s) 236, 240, 765, 1052, 1065, 2031
Tru9I TTAA 6 cut(s) 236, 240, 765, 1052, 1065, 2031
TscAI CASTG 3 cut(s) 235, 1328, 2128
TseFI GTSAC 2 cut(s) 439, 1399
TseI GCWGC 6 cut(s) 194, 742, 1295, 2078, 2347, 2417
Tsp45I GTSAC 2 cut(s) 439, 1399
TspGWI ACGGA 1 cut(s) 209
TspMI CCCGGG 1 cut(s) 800
TspRI CASTG 3 cut(s) 235, 1328, 2128
Tth111I GACNNNGTC 2 cut(s) 1511, 1856
Vha464I CTTAAG 1 cut(s) 1064
VpaK11BI GGWCC 3 cut(s) 872, 1613, 1691
XagI CCTNNNNNAGG 1 cut(s) 1593
XapI RAATTY 4 cut(s) 1387, 1574, 1822, 2372
XceI RCATGY 3 cut(s) 1082, 1585, 2040
XcmI CCANNNNNNNNNTGG 3 cut(s) 1277, 1482, 1501
XmaI CCCGGG 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 1413
XspI CTAG 3 cut(s) 653, 1796, 2075
ZraI GACGTC 1 cut(s) 1973
ZrmI AGTACT 1 cut(s) 37
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.