pycom06g15170

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013824
Physical Location & Seq
Reverse (-)
19766773 .. 19770753
3981 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g15170.9

Sequence Viewer

Length: 2394 bp
ATGGCGCCACCTAGTCCATCTGCTCCTGATCAATCCCAGCCGTCTTCGTCCTTGGACCCGAAAAGTAACAAGAAAGATTTTTCGACCGCCATTTCGGAACGAAAGAAGTCTCCTAATCGCCTTGTTGTTGATGAAGCTGTCAATGATGATAATTCTGTGATTTCCTTGCACCCTTCTGCAATGGAAAAGCTTCAGTTTTTCCGTGGAGACACTGTTTTAATTAAGGGGAAGAAAAGGAGAGACACAATTTGCATTGTTATGGCTGATAACGAATGCGAGGAGCCGAAAATCAGAATGAACAAAGTTGTGAGAGCTAATCTTAGGGTTCGCCTCGGGGATATTGTGTCTGTTCATCTCTGCCATGATGTCAAGTATGGAAAGAAGGTTCACATCCTTCCAATCGATGATACAATTGAAGGCGTGACAGGAAGCTTATTCGATGCATATTTGAAGCCATACTTTGCGGAAGCTTACCGCCCTGTGAGGAAGGGTGATCTTTTCCTGGTTCAGGGCATGATGCGAAGCATTGAGTTCAAGATCATTGAGACAGACCCTGATGAGTACTGCATTGTTGCACCGGATACTGAGATTTTCTGTGAGGGAGAGCCTGTCAAACGCGAGGACGAGGAAAGACAAGAGGATGTAGGATATGATGATGTTGGAGGTGTTCGAAAGCAAATGGCTCAGATACGCGAGTTAGTAGAACTGCCTCTTAGACATCCACAGCTCTTCGAATCGATTGGAGTGAAACCCCCGAAGGGGATTTTACTCTATGGGCCACCCGGGACGGGGAAAACATTGATTGCAAGGGCTGTAGCAAATGAGACTGGAGCGTTCTTCTTTTCGATCAATGGACCTGAAATAATGTCCAAGATGGCAGGTGAAAGTGAAAGCAACTTGAGGAAGGCGTTTGAGGAAGCTGAAAAGAATGCACCCTCAATCATTTTTATTGATGAGATAGATTCTATTGCTCCGAAGAGGGAAAAGACCCACGGTGAAGTGGGAAGGCGTATAGTTTCCCAGCTCCTGACTTTAATGGACGGCCTTAAGTCCCGTGCGCATGTCATTGTAATGGGAGCAACGAATAGGCCAAACAGCATCGACCCTGCTCTAAGGAGATTTGGAAGATTCGATAGAGAGATTGACATTGGTGTCCCGGATGAAGTTGGAAGATTGGAAGTTTTGCGAATCCATACAAAGAGGATGAAACTTGCAGATGATGTTGATCTCGAACGAGTTGCAAAAGATACCCATGGTTTTGTTGGTGCAGATCTTGCCGCTCTCTCCACAGAGGCTGCGCTCCAGTGCATCCGTGAAAAAATGGACGTTATTGACTTGGAAGACGATACGATTGATGCTGAGGTGTTGAATTCGATGGCTGTGACGAATGAACACTTCCAGACCGCACTGTCCTCTACAAATCCTTCTGCCTTGCGCGAAACTGTTGTGGACGTACCTAATGTTTCGTGGGATGCCATTGGCGGGTTGGAGAACGTCAAAAGAGAACTTCAAGAGGTTTGCGTTCTCTTTTATGGACCACCTGGCTGTGGGAAAACCCTTCTCGCCAAGGCAATTGCCAATGAATGTCAGGCCAACTTCATCAGTATCAAAGGACCTGAGCTGCTAACAATGTGGTTCGGAGAAAGTGAGGCAAATGTGCGCGAGATATTTGACAAGGCTCGCCAATCCGCTCCTTGTGTTCTTTTCTTTGATGAGCTAGACTCAATTGCAACTCAGCGTGGCAATTCTGTGGGGGATGCTGGTGGTGCTGGAGACAGAGTCTTGAACCAACTTCTAACCGAAATGGATGGAATGAATGCAAAGAAAACCGTCTTTGTCATTGGGGCGACAAACAGGCCAGACATTATTGACCCGGCATTGCTCAGGCCTGGACGTCTTGACCAACTTATCTACATCCCGCTCCCAGATGAGGCTTCGCGCCTTCAGATTTTTAAAGCATGTCTGCGCAAGTCACCCATCTCTAAGGATATTGACCTAGCAGCTCTTGCCCGTTATACGCGCGGTTTTAGTGGTGCAGATATCACCGAGATATGTCAACGTGCCTGCAAATATGCCATCAGAGAAGACATTGAGAAGGCTATTGAGAGGCAAAGAAGAAAGAACGAGAACTCCGAAGCCATGGAGGAAGTTGAAACTGATGAGGGACCCGAGATCAAGCCAGTGCACTTTGAGGAATCGATGAAACATGCCCGAAGAAGTGTGAGTGATGCAGACATCAAGAAGTACCAGGTGTTTGCTCAGACTTTGCAACAATCTCGTGGGTTTGGATCCGAATTTCGATTTCCACAGACAGAGAACAATGGAGGAGACGCAGCTCCAACCCCATTTTCTTCAGCCACAGTTGCTGGAGAGGAAGATGATCTCTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

798

Amino Acids

88.49

Weight (kDa)

5.14

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 869
AasI GACNNNNNNGTC 2 cut(s) 1151, 1408
AatII GACGTC 1 cut(s) 1897
Acc16I TGCGCA 2 cut(s) 1059, 1967
Acc36I ACCTGC 1 cut(s) 869
AccB1I GGYRCC 1 cut(s) 4
AccBSI CCGCTC 3 cut(s) 1280, 1691, 1921
AccII CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
AciI CCGC 9 cut(s) 87, 464, 475, 1278, 1404, 1482, 1689, 1919, 2022
AclWI GGATC 2 cut(s) 2283, 2296
AcsI RAATTY 2 cut(s) 1369, 2294
AcuI CTGAAG 3 cut(s) 176, 1928, 2337
AcyI GRCGYC 2 cut(s) 5, 1894
AfaI GTAC 3 cut(s) 563, 1455, 2246
AfiI CCNNNNNNNGG 8 cut(s) 508, 758, 759, 788, 789, 1482, 1547, 1930
AflII CTTAAG 1 cut(s) 1046
AgsI TTSAA 7 cut(s) 416, 451, 535, 1369, 1511, 1786, 2153
AjnI CCWGG 4 cut(s) 501, 1540, 1888, 2247
AjuI GAANNNNNNNTTGG 2 cut(s) 2332, 2364
AloI GAACNNNNNNTCC 4 cut(s) 369, 401, 2114, 2146
Alw21I GWGCWC 1 cut(s) 2187
Alw26I GTCTC 7 cut(s) 114, 201, 234, 539, 818, 1767, 2322
Alw44I GTGCAC 1 cut(s) 2183
AlwI GGATC 2 cut(s) 2283, 2296
AlwNI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2366
Ama87I CYCGRG 3 cut(s) 332, 782, 2168
AoxI GGCC 6 cut(s) 776, 1042, 1088, 1590, 1856, 1886
ApaLI GTGCAC 1 cut(s) 2183
ApeKI GCWGC 4 cut(s) 1295, 1621, 2000, 2333
ApoI RAATTY 2 cut(s) 1369, 2294
ArsI GACNNNNNNTTYG 2 cut(s) 76, 108
Asp700I GAANNNNTTC 1 cut(s) 189
AspLEI GCGC 8 cut(s) 7, 1060, 1300, 1437, 1662, 1941, 1968, 2022
AspS9I GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
AsuC2I CCSGG 4 cut(s) 783, 784, 1157, 1874
AsuHPI GGTGA 5 cut(s) 503, 893, 1007, 1965, 2035
AsuII TTCGAA 2 cut(s) 670, 732
AvaI CYCGRG 3 cut(s) 332, 782, 2168
AvaII GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
BaeGI GKGCMC 1 cut(s) 2187
BaeI ACNNNNGTAYC 2 cut(s) 680, 713
BamHI GGATCC 1 cut(s) 2288
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 2277
BbsI GAAGAC 3 cut(s) 36, 1347, 2091
Bbv12I GWGCWC 1 cut(s) 2187
BbvCI CCTCAGC 1 cut(s) 1359
BbvI GCAGC 4 cut(s) 1282, 1608, 2012, 2345
BccI CCATC 6 cut(s) 25, 868, 1369, 1802, 1985, 2084
BceAI ACGGC 2 cut(s) 25, 1057
BcgI CGANNNNNNTGC 4 cut(s) 1220, 1254, 2258, 2292
BciT130I CCWGG 4 cut(s) 503, 1542, 1890, 2249
BciVI GTATCC 1 cut(s) 574
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 4 cut(s) 783, 784, 1157, 1874
BcoDI GTCTC 7 cut(s) 114, 201, 234, 539, 818, 1767, 2322
BfaI CTAG 3 cut(s) 12, 1718, 1997
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 1046
BfuAI ACCTGC 1 cut(s) 869
BfuI GTATCC 1 cut(s) 574
BglII AGATCT 1 cut(s) 1270
BisI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
BlsI GCNGC 5 cut(s) 1279, 1297, 1623, 2002, 2335
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 8 cut(s) 503, 783, 784, 1157, 1542, 1874, 1890, 2249
Bme18I GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
BmeT110I CYCGRG 3 cut(s) 332, 782, 2168
BmgT120I GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
BmiI GGNNCC 6 cut(s) 6, 57, 282, 2166, 2167, 2290
BmrFI CCNGG 8 cut(s) 503, 783, 784, 1157, 1542, 1874, 1890, 2249
BmsI GCATC 8 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1747, 2218
BpiI GAAGAC 3 cut(s) 36, 1347, 2091
BplI GAGNNNNNCTC 2 cut(s) 1706, 1738
BpmI CTGGAG 4 cut(s) 849, 1286, 1791, 2388
Bpu10I CCTNAGC 3 cut(s) 1359, 1617, 1883
Bpu14I TTCGAA 2 cut(s) 670, 732
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 4 cut(s) 783, 784, 1157, 1874
Bsa29I ATCGAT 3 cut(s) 402, 737, 2198
BsaBI GATNNNNATC 1 cut(s) 1224
BsaHI GRCGYC 2 cut(s) 5, 1894
BsaJI CCNNGG 8 cut(s) 51, 202, 331, 782, 991, 1252, 1566, 2139
BsaWI WCCGGW 1 cut(s) 577
BsaXI ACNNNNNCTCC 8 cut(s) 198, 228, 594, 624, 1764, 1794, 2114, 2144
Bsc4I CCNNNNNNNGG 8 cut(s) 508, 758, 759, 788, 789, 1482, 1547, 1930
Bse1I ACTGG 3 cut(s) 832, 1303, 2180
Bse3DI GCAATG 2 cut(s) 186, 1877
Bse8I GATNNNNATC 1 cut(s) 1224
BseBI CCWGG 4 cut(s) 503, 1542, 1890, 2249
BseCI ATCGAT 3 cut(s) 402, 737, 2198
BseDI CCNNGG 8 cut(s) 51, 202, 331, 782, 991, 1252, 1566, 2139
BseJI GATNNNNATC 1 cut(s) 1224
BseLI CCNNNNNNNGG 8 cut(s) 508, 758, 759, 788, 789, 1482, 1547, 1930
BseMI GCAATG 2 cut(s) 186, 1877
BseMII CTCAG 7 cut(s) 576, 698, 1350, 1608, 1748, 1897, 2273
BseNI ACTGG 3 cut(s) 832, 1303, 2180
BseRI GAGGAG 2 cut(s) 293, 2340
BseSI GKGCMC 1 cut(s) 2187
BseXI GCAGC 4 cut(s) 1282, 1608, 2012, 2345
BseYI CCCAGC 2 cut(s) 36, 1020
BsgI GTGCAG 2 cut(s) 1287, 2055
Bsh1236I CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 3 cut(s) 402, 737, 2198
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 2187
BsiHKCI CYCGRG 3 cut(s) 332, 782, 2168
BsiSI CCGG 4 cut(s) 578, 783, 1157, 1874
BslFI GGGAC 4 cut(s) 799, 1036, 1139, 2178
BslI CCNNNNNNNGG 8 cut(s) 508, 758, 759, 788, 789, 1482, 1547, 1930
BsmAI GTCTC 7 cut(s) 114, 201, 234, 539, 818, 1767, 2322
BsmBI CGTCTC 1 cut(s) 2322
BsmFI GGGAC 4 cut(s) 799, 1036, 1139, 2178
BsmI GAATGC 3 cut(s) 278, 934, 1822
BsnI GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
BsoBI CYCGRG 3 cut(s) 332, 782, 2168
Bsp119I TTCGAA 2 cut(s) 670, 732
Bsp1286I GDGCHC 1 cut(s) 2187
Bsp143I GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
Bsp19I CCATGG 2 cut(s) 1252, 2139
BspACI CCGC 9 cut(s) 87, 464, 475, 1278, 1404, 1482, 1689, 1919, 2022
BspANI GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
BspCNI CTCAG 7 cut(s) 577, 697, 1351, 1609, 1747, 1896, 2272
BspDI ATCGAT 3 cut(s) 402, 737, 2198
BspFNI CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
BspLI GGNNCC 6 cut(s) 6, 57, 282, 2166, 2167, 2290
BspMI ACCTGC 1 cut(s) 869
BspPI GGATC 2 cut(s) 2283, 2296
BspQI GCTCTTC 1 cut(s) 734
BspT104I TTCGAA 2 cut(s) 670, 732
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 1046
BsrBI CCGCTC 3 cut(s) 1280, 1691, 1921
BsrDI GCAATG 2 cut(s) 186, 1877
BsrI ACTGG 3 cut(s) 832, 1303, 2180
BssECI CCNNGG 8 cut(s) 51, 202, 331, 782, 991, 1252, 1566, 2139
BssMI GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
BssNI GRCGYC 2 cut(s) 5, 1894
BssSI CACGAG 1 cut(s) 2277
BssT1I CCWWGG 4 cut(s) 51, 1252, 1566, 2139
Bst2BI CACGAG 1 cut(s) 2277
Bst2UI CCWGG 4 cut(s) 503, 1542, 1890, 2249
Bst4CI ACNGT 6 cut(s) 214, 995, 1410, 1444, 1831, 2362
Bst6I CTCTTC 2 cut(s) 734, 971
BstACI GRCGYC 2 cut(s) 5, 1894
BstAFI CTTAAG 1 cut(s) 1046
BstAPI GCANNNNNTGC 2 cut(s) 1274, 2006
BstBI TTCGAA 2 cut(s) 670, 732
BstC8I GCNNGC 2 cut(s) 1681, 2065
BstDSI CCRYGG 4 cut(s) 202, 991, 1252, 2139
BstENI CCTNNNNNAGG 1 cut(s) 506
BstFNI CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 8 cut(s) 7, 1060, 1300, 1437, 1662, 1941, 1968, 2022
BstKTI GATC 9 cut(s) 31, 496, 540, 849, 1228, 1273, 2175, 2291, 2383
BstMAI GTCTC 7 cut(s) 114, 201, 234, 539, 818, 1767, 2322
BstMBI GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 4 cut(s) 1274, 1766, 2006, 2363
BstNI CCWGG 4 cut(s) 503, 1542, 1890, 2249
BstNSI RCATGY 3 cut(s) 1064, 1962, 2210
BstSCI CCNGG 8 cut(s) 501, 781, 782, 1155, 1540, 1872, 1888, 2247
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 2187
BstUI CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
BstV1I GCAGC 4 cut(s) 1282, 1608, 2012, 2345
BstV2I GAAGAC 3 cut(s) 36, 1347, 2091
BstX2I RGATCY 2 cut(s) 1270, 2288
BstYI RGATCY 2 cut(s) 1270, 2288
Bsu15I ATCGAT 3 cut(s) 402, 737, 2198
BsuI GTATCC 1 cut(s) 574
BsuRI GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
BsuTUI ATCGAT 3 cut(s) 402, 737, 2198
BtgI CCRYGG 4 cut(s) 202, 991, 1252, 2139
BtsIMutI CAGTG 4 cut(s) 210, 1310, 1406, 2187
BveI ACCTGC 1 cut(s) 869
Cac8I GCNNGC 2 cut(s) 1681, 2065
CaiI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2366
CfoI GCGC 8 cut(s) 7, 1060, 1300, 1437, 1662, 1941, 1968, 2022
Cfr13I GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
Cfr9I CCCGGG 1 cut(s) 782
ClaI ATCGAT 3 cut(s) 402, 737, 2198
CseI GACGC 1 cut(s) 2339
CsiI ACCWGGT 1 cut(s) 2247
Csp6I GTAC 3 cut(s) 562, 1454, 2245
CviAII CATG 7 cut(s) 362, 514, 1061, 1253, 1959, 2140, 2207
CviQI GTAC 3 cut(s) 562, 1454, 2245
DinI GGCGCC 1 cut(s) 6
DpnI GATC 9 cut(s) 30, 495, 539, 848, 1227, 1272, 2174, 2290, 2382
DpnII GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
DraI TTTAAA 1 cut(s) 1954
DrdI GACNNNNNNGTC 2 cut(s) 1151, 1408
DseDI GACNNNNNNGTC 2 cut(s) 1151, 1408
Eam1104I CTCTTC 2 cut(s) 734, 971
EarI CTCTTC 2 cut(s) 734, 971
Eco130I CCWWGG 4 cut(s) 51, 1252, 1566, 2139
Eco147I AGGCCT 1 cut(s) 1888
Eco32I GATATC 1 cut(s) 2041
Eco47I GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
Eco57I CTGAAG 3 cut(s) 176, 1928, 2337
Eco88I CYCGRG 3 cut(s) 332, 782, 2168
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoO109I RGGNCCY 2 cut(s) 1613, 2165
EcoRI GAATTC 1 cut(s) 1369
EcoRII CCWGG 4 cut(s) 501, 1540, 1888, 2247
EcoRV GATATC 1 cut(s) 2041
EcoT14I CCWWGG 4 cut(s) 51, 1252, 1566, 2139
EcoT22I ATGCAT 1 cut(s) 445
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 51, 1252, 1566, 2139
Esp3I CGTCTC 1 cut(s) 2322
FaeI CATG 7 cut(s) 365, 517, 1064, 1256, 1962, 2143, 2210
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 4 cut(s) 799, 1036, 1139, 2178
FatI CATG 7 cut(s) 361, 513, 1060, 1252, 1958, 2139, 2206
FauI CCCGC 2 cut(s) 1475, 1926
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
Fsp4HI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
FspAI RTGCGCAY 1 cut(s) 1059
FspBI CTAG 3 cut(s) 12, 1718, 1997
FspI TGCGCA 2 cut(s) 1059, 1967
GlaI GCGC 8 cut(s) 6, 1059, 1299, 1436, 1661, 1940, 1967, 2021
GluI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
GsaI CCCAGC 2 cut(s) 40, 1024
GsuI CTGGAG 4 cut(s) 849, 1286, 1791, 2388
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
HapII CCGG 4 cut(s) 578, 783, 1157, 1874
HgaI GACGC 1 cut(s) 2339
HhaI GCGC 8 cut(s) 7, 1060, 1300, 1437, 1662, 1941, 1968, 2022
Hin1I GRCGYC 2 cut(s) 5, 1894
Hin1II CATG 7 cut(s) 365, 517, 1064, 1256, 1962, 2143, 2210
Hin6I GCGC 8 cut(s) 5, 1058, 1298, 1435, 1660, 1939, 1966, 2020
HinP1I GCGC 8 cut(s) 5, 1058, 1298, 1435, 1660, 1939, 1966, 2020
HincII GTYRAC 1 cut(s) 2057
HindII GTYRAC 1 cut(s) 2057
HindIII AAGCTT 3 cut(s) 188, 430, 468
HinfI GANTC 7 cut(s) 734, 962, 1128, 1188, 1721, 1779, 2195
HpaII CCGG 4 cut(s) 578, 783, 1157, 1874
HphI GGTGA 5 cut(s) 503, 893, 1007, 1965, 2035
Hpy166II GTNNAC 4 cut(s) 388, 1450, 2057, 2185
Hpy188III TCNNGA 9 cut(s) 26, 535, 1027, 1229, 1399, 1511, 1783, 1898, 2239
Hpy8I GTNNAC 4 cut(s) 388, 1450, 2057, 2185
HpyCH4III ACNGT 6 cut(s) 214, 995, 1410, 1444, 1831, 2362
HpyCH4IV ACGT 5 cut(s) 1326, 1452, 1494, 1894, 2059
HpyF10VI GCNNNNNNNGC 4 cut(s) 1274, 1766, 2006, 2363
HpySE526I ACGT 5 cut(s) 1326, 1452, 1494, 1894, 2059
Hsp92I GRCGYC 2 cut(s) 5, 1894
Hsp92II CATG 7 cut(s) 365, 517, 1064, 1256, 1962, 2143, 2210
HspAI GCGC 8 cut(s) 5, 1058, 1298, 1435, 1660, 1939, 1966, 2020
KasI GGCGCC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 2165
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
LguI GCTCTTC 1 cut(s) 734
Lsp1109I GCAGC 4 cut(s) 1282, 1608, 2012, 2345
LweI GCATC 8 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1747, 2218
MabI ACCWGGT 1 cut(s) 2247
MaeI CTAG 3 cut(s) 12, 1718, 1997
MaeII ACGT 5 cut(s) 1326, 1452, 1494, 1894, 2059
MaeIII GTNAC 4 cut(s) 65, 421, 1381, 1971
MalI GATC 9 cut(s) 30, 495, 539, 848, 1227, 1272, 2174, 2290, 2382
MbiI CCGCTC 3 cut(s) 1280, 1691, 1921
MboI GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
MfeI CAATTG 3 cut(s) 411, 1572, 1725
MflI RGATCY 2 cut(s) 1270, 2288
MhlI GDGCHC 1 cut(s) 2187
MluCI AATT 9 cut(s) 151, 219, 246, 411, 1369, 1572, 1725, 1744, 2294
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 1715, 1788
MmeI TCCRAC 4 cut(s) 640, 1147, 1467, 2363
Mph1103I ATGCAT 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 189
MseI TTAA 5 cut(s) 218, 222, 1034, 1047, 1953
MslI CAYNNNNRTG 2 cut(s) 257, 1070
MspCI CTTAAG 1 cut(s) 1046
MspI CCGG 4 cut(s) 578, 783, 1157, 1874
MspR9I CCNGG 8 cut(s) 503, 783, 784, 1157, 1542, 1874, 1890, 2249
MunI CAATTG 3 cut(s) 411, 1572, 1725
Mva1269I GAATGC 3 cut(s) 278, 934, 1822
MvaI CCWGG 4 cut(s) 503, 1542, 1890, 2249
MvnI CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
MwoI GCNNNNNNNGC 4 cut(s) 1274, 1766, 2006, 2363
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 783, 784, 1157, 1874
NcoI CCATGG 2 cut(s) 1252, 2139
NdeII GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
NlaIII CATG 7 cut(s) 365, 517, 1064, 1256, 1962, 2143, 2210
NlaIV GGNNCC 6 cut(s) 6, 57, 282, 2166, 2167, 2290
NmuCI GTSAC 3 cut(s) 421, 1381, 1971
NsbI TGCGCA 2 cut(s) 1059, 1967
NsiI ATGCAT 1 cut(s) 445
NspI RCATGY 3 cut(s) 1064, 1962, 2210
NspV TTCGAA 2 cut(s) 670, 732
PacI TTAATTAA 1 cut(s) 222
PaqCI CACCTGC 1 cut(s) 869
PceI AGGCCT 1 cut(s) 1888
PciSI GCTCTTC 1 cut(s) 734
PcsI WCGNNNNNNNCGW 1 cut(s) 2130
PctI GAATGC 3 cut(s) 278, 934, 1822
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 5 cut(s) 734, 962, 1128, 1188, 2195
PflFI GACNNNGTC 1 cut(s) 1778
PfoI TCCNGGA 1 cut(s) 1155
PkrI GCNGC 5 cut(s) 1279, 1297, 1623, 2002, 2335
PleI GAGTC 2 cut(s) 1715, 1787
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 1715, 1787
PpuMI RGGWCCY 2 cut(s) 1613, 2165
Psp5II RGGWCCY 2 cut(s) 1613, 2165
Psp6I CCWGG 4 cut(s) 501, 1540, 1888, 2247
PspFI CCCAGC 2 cut(s) 36, 1020
PspGI CCWGG 4 cut(s) 501, 1540, 1888, 2247
PspN4I GGNNCC 6 cut(s) 6, 57, 282, 2166, 2167, 2290
PspPI GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
PspPPI RGGWCCY 2 cut(s) 1613, 2165
PstNI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2366
PsuI RGATCY 2 cut(s) 1270, 2288
PsyI GACNNNGTC 1 cut(s) 1778
RsaI GTAC 3 cut(s) 563, 1455, 2246
RsaNI GTAC 3 cut(s) 562, 1454, 2245
RseI CAYNNNNRTG 2 cut(s) 257, 1070
SapI GCTCTTC 1 cut(s) 734
SaqAI TTAA 5 cut(s) 218, 222, 1034, 1047, 1953
SatI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
Sau3AI GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
Sau96I GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 2 cut(s) 1715, 1788
ScrFI CCNGG 8 cut(s) 503, 783, 784, 1157, 1542, 1874, 1890, 2249
SduI GDGCHC 1 cut(s) 2187
SexAI ACCWGGT 1 cut(s) 2247
SfaNI GCATC 8 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1747, 2218
SfcI CTRYAG 1 cut(s) 813
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 2 cut(s) 670, 732
SinI GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
SmaI CCCGGG 1 cut(s) 784
SmiMI CAYNNNNRTG 2 cut(s) 257, 1070
SmlI CTYRAG 2 cut(s) 898, 1046
SmoI CTYRAG 2 cut(s) 898, 1046
Sse9I AATT 9 cut(s) 151, 219, 246, 411, 1369, 1572, 1725, 1744, 2294
SseBI AGGCCT 1 cut(s) 1888
SsiI CCGC 9 cut(s) 87, 464, 475, 1278, 1404, 1482, 1689, 1919, 2022
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 12, 1718, 1997
StuI AGGCCT 1 cut(s) 1888
StyD4I CCNGG 8 cut(s) 501, 781, 782, 1155, 1540, 1872, 1888, 2247
StyI CCWWGG 4 cut(s) 51, 1252, 1566, 2139
TaaI ACNGT 6 cut(s) 214, 995, 1410, 1444, 1831, 2362
TaiI ACGT 5 cut(s) 1329, 1455, 1497, 1897, 2062
TasI AATT 9 cut(s) 151, 219, 246, 411, 1369, 1572, 1725, 1744, 2294
TatI WGTACW 1 cut(s) 561
TauI GCSGC 1 cut(s) 1280
TfiI GAWTC 5 cut(s) 734, 962, 1128, 1188, 2195
Tru1I TTAA 5 cut(s) 218, 222, 1034, 1047, 1953
Tru9I TTAA 5 cut(s) 218, 222, 1034, 1047, 1953
TscAI CASTG 4 cut(s) 217, 1310, 1413, 2187
TseFI GTSAC 3 cut(s) 421, 1381, 1971
TseI GCWGC 4 cut(s) 1295, 1621, 2000, 2333
Tsp45I GTSAC 3 cut(s) 421, 1381, 1971
TspGWI ACGGA 2 cut(s) 191, 1301
TspMI CCCGGG 1 cut(s) 782
TspRI CASTG 4 cut(s) 217, 1310, 1413, 2187
Tth111I GACNNNGTC 1 cut(s) 1778
Vha464I CTTAAG 1 cut(s) 1046
VneI GTGCAC 1 cut(s) 2183
VpaK11BI GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
XagI CCTNNNNNAGG 1 cut(s) 506
XapI RAATTY 2 cut(s) 1369, 2294
XceI RCATGY 3 cut(s) 1064, 1962, 2210
XcmI CCANNNNNNNNNTGG 2 cut(s) 1259, 1483
XmaI CCCGGG 1 cut(s) 782
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 3 cut(s) 12, 1718, 1997
ZraI GACGTC 1 cut(s) 1895
ZrmI AGTACT 1 cut(s) 563
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.