Rh7AG132400

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0013824
Physical Location & Seq
Reverse (-)
10619164 .. 10623466
4303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG132400.1

Sequence Viewer

Length: 1539 bp
ATGGCGGATCCGAGCTCATCCGCCCCAAATCCTGACCCGAAAACTAAGAAGGATTTTTCCACTGCAATTTTGGAGCGAAAGAAGTCTCCTAATCGACTTGTGGTTGATGAAGCTATCAATGATGATAATTCTGTGGTGTCCTTGCACCCTGCTACCATGGAAAAACTTCAGTTTTTTCGGGGTGACACTGTGCTAATAAAGGGGAAGAAACGAAAAGACACAGTTTGCATTGTTCTTGCTGATGAACAATGTGAAGAACCGAAAATCAGAATGAACAAAGTTGTAAGGGCTAATCTTAGGGTTCGTCTTGGAGATGTTGTGTCGGTTCATACTTGCCCTGATGTCAAATATGGGAAGCGGGTTCACATCCTTCCAATCGATGATACAATTGAAGGCGTAACTGGAAACTTGTTTGATGCATATTTGAAGCCATACTTCTTGGAATCTTACCGGCCTGTGAGGAAGGGTGACCTTTTCCTGGTCAGGGGTGGGATGCGAAGTGTTGAGTTCAAGGTCATTGAGACAGACCCTGGTGAATACTGTGTTGTTGCTCCCGATACTGAGATTTTCTGTGAGGGAGAACCTGTGAAACGTGAGGATGAGGAAAAATTGAATGAGGTGGGCTATGATGATGTTGGAGGTGTTAGGAAGCAAATGGCTCAGATACGCGAGTTAGTAGAGTTACCTCTTAGACACCCACAGCTCTTCAAGTCCATTGGAGTGAAACCACCTAAAGGTATTCTGCTTTATGGACCACCTGGGTCTGGGAAAACTTTGATTGCAAGGGCTGTAGCTAATGAGACTGGAGCATTCTTCTTTTTGATTAATGGGCCTGAAATAATGTCAAAGTTAGCTGGTGAGAGTGAAAGCAACTTGAGGAAGGCATTTGAAGAAGCTGAAAAGAATGCCCCATCAATCATTTTTATTGATGAGTTAGATTCCATTGCTCCAAAGAGGGAGAAGACTCAGGGTGAAGTGGAAAGGCGTATAGTATCGCAACTTCTGACTTTGATGGATGGTCTCAAGTCCCGTGCACATGTTATTGTCATGGGAGCTACAAATAGGCCAAACAGCATTGACCCTGCTCTGAGGAGGTTTGGAAGATTTGATAGAGAGATTGACATAGGTGTACCGGATGAAGTTGGAAGATTGGAAGTTTTGAGAATCCATACAAAGAACATGAAACTTGCAGAAGATGTTGATCTCGAACGAGTTTCAAAGGATACCCATGGTTATGTCGGTGCTGATCTTGCCGCTCTCTGTACTGAGGCTGCTCTTCAGTGCATCCGTGAGAAAATGGATGTTATTGACTTGGAAGATGAGGAAATTGATGCTGAGGTGTTGAATTCTATGGCTGTGACGAATGAACACTTCCAGACTGCATTGGGTGCTTCAAATCCTTCAGCATTGCGTGAAACTGTGAGTGACAAAGTTTTACATGTTTTACTTATTTTCTGGATGTTGCTAATCCTTCAGCATTGCGTGAAACTGCGAGTGACAAAGTTTTACATGTTTTACTTATTTTCTGGATGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

512

Amino Acids

57.41

Weight (kDa)

5.75

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 32 - 113 6.3e-24 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 133 - 197 6.5e-12 Cell division protein 48 (CDC48), domain 2
AAA PF00004 247 - 376 6.5e-48 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 399 - 442 6.8e-16 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1256
AccII CGCG 1 cut(s) 669
AciI CCGC 4 cut(s) 5, 21, 358, 1254
AclWI GGATC 2 cut(s) 2, 15
AcsI RAATTY 1 cut(s) 1345
AcuI CTGAAG 4 cut(s) 152, 1262, 1386, 1457
AfaI GTAC 2 cut(s) 1131, 1264
AfiI CCNNNNNNNGG 4 cut(s) 478, 484, 734, 764
AflIII ACRYGT 3 cut(s) 1036, 1438, 1509
AgsI TTSAA 9 cut(s) 392, 427, 511, 613, 709, 890, 1218, 1345, 1395
AjnI CCWGG 3 cut(s) 477, 529, 757
AluBI AGCT 7 cut(s) 15, 113, 703, 794, 854, 896, 1055
AluI AGCT 7 cut(s) 15, 113, 703, 794, 854, 896, 1055
Alw21I GWGCWC 2 cut(s) 17, 1036
Alw26I GTCTC 4 cut(s) 90, 515, 794, 1025
Alw44I GTGCAC 1 cut(s) 1032
AlwI GGATC 2 cut(s) 2, 15
AlwNI CAGNNNCTG 1 cut(s) 530
AoxI GGCC 3 cut(s) 452, 830, 1064
ApaLI GTGCAC 1 cut(s) 1032
ApeKI GCWGC 1 cut(s) 1271
ApoI RAATTY 1 cut(s) 1345
AseI ATTAAT 1 cut(s) 825
Asp700I GAANNNNTTC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 752, 830
AsuHPI GGTGA 5 cut(s) 194, 479, 545, 869, 983
AvaII GGWCC 1 cut(s) 752
BaeGI GKGCMC 1 cut(s) 1036
BaeI ACNNNNGTAYC 2 cut(s) 656, 689
BamHI GGATCC 1 cut(s) 7
BanII GRGCYC 1 cut(s) 17
BarI GAAGNNNNNNTAC 2 cut(s) 984, 1016
BbsI GAAGAC 1 cut(s) 968
Bbv12I GWGCWC 2 cut(s) 17, 1036
BbvCI CCTCAGC 1 cut(s) 1335
BbvI GCAGC 1 cut(s) 1258
BccI CCATC 3 cut(s) 919, 1006, 1010
BciT130I CCWGG 3 cut(s) 479, 531, 759
BciVI GTATCC 1 cut(s) 1216
BcoDI GTCTC 4 cut(s) 90, 515, 794, 1025
BfmI CTRYAG 1 cut(s) 789
BfuI GTATCC 1 cut(s) 1216
BisI GCNGC 2 cut(s) 1254, 1272
BlsI GCNGC 2 cut(s) 1255, 1273
Bme1390I CCNGG 3 cut(s) 479, 531, 759
Bme18I GGWCC 1 cut(s) 752
BmgT120I GGNCC 2 cut(s) 752, 830
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 3 cut(s) 479, 531, 759
BmsI GCATC 4 cut(s) 406, 483, 1293, 1321
BpiI GAAGAC 1 cut(s) 968
BpmI CTGGAG 1 cut(s) 825
Bpu10I CCTNAGC 1 cut(s) 1335
BpuEI CTTGAG 2 cut(s) 895, 1007
Bsa29I ATCGAT 1 cut(s) 378
BsaBI GATNNNNATC 1 cut(s) 1200
BsaI GGTCTC 1 cut(s) 1025
BsaJI CCNNGG 4 cut(s) 156, 529, 758, 1228
BsaWI WCCGGW 1 cut(s) 1132
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 570, 600
Bsc4I CCNNNNNNNGG 4 cut(s) 478, 484, 734, 764
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 2 cut(s) 406, 808
Bse3DI GCAATG 3 cut(s) 942, 1406, 1477
Bse8I GATNNNNATC 1 cut(s) 1200
BseBI CCWGG 3 cut(s) 479, 531, 759
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 4 cut(s) 156, 529, 758, 1228
BseJI GATNNNNATC 1 cut(s) 1200
BseLI CCNNNNNNNGG 4 cut(s) 478, 484, 734, 764
BseMI GCAATG 3 cut(s) 942, 1406, 1477
BseMII CTCAG 6 cut(s) 552, 674, 980, 1079, 1257, 1326
BseNI ACTGG 2 cut(s) 406, 808
BseRI GAGGAG 1 cut(s) 1105
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 1 cut(s) 1258
Bsh1236I CGCG 1 cut(s) 669
BshFI GGCC 3 cut(s) 454, 832, 1066
BshVI ATCGAT 1 cut(s) 378
BsiHKAI GWGCWC 2 cut(s) 17, 1036
BsiSI CCGG 2 cut(s) 451, 1133
BslFI GGGAC 1 cut(s) 1012
BslI CCNNNNNNNGG 4 cut(s) 478, 484, 734, 764
BsmAI GTCTC 4 cut(s) 90, 515, 794, 1025
BsmFI GGGAC 1 cut(s) 1012
BsmI GAATGC 2 cut(s) 809, 910
BsnI GGCC 3 cut(s) 454, 832, 1066
Bso31I GGTCTC 1 cut(s) 1025
Bsp1286I GDGCHC 2 cut(s) 17, 1036
Bsp143I GATC 3 cut(s) 7, 1201, 1246
Bsp19I CCATGG 2 cut(s) 156, 1228
BspACI CCGC 4 cut(s) 5, 21, 358, 1254
BspANI GGCC 3 cut(s) 454, 832, 1066
BspCNI CTCAG 6 cut(s) 553, 673, 979, 1080, 1258, 1327
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 1 cut(s) 669
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 2 cut(s) 2, 15
BspQI GCTCTTC 2 cut(s) 710, 1281
BspTNI GGTCTC 1 cut(s) 1025
BsrBI CCGCTC 1 cut(s) 1256
BsrDI GCAATG 3 cut(s) 942, 1406, 1477
BsrFI RCCGGY 1 cut(s) 450
BsrI ACTGG 2 cut(s) 406, 808
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 4 cut(s) 156, 529, 758, 1228
BssMI GATC 3 cut(s) 7, 1201, 1246
BssT1I CCWWGG 2 cut(s) 156, 1228
Bst2UI CCWGG 3 cut(s) 479, 531, 759
Bst4CI ACNGT 4 cut(s) 190, 223, 542, 1420
Bst6I CTCTTC 2 cut(s) 710, 1281
BstAPI GCANNNNNTGC 1 cut(s) 1388
BstDEI CTNAG 9 cut(s) 45, 296, 561, 660, 689, 966, 1088, 1266, 1335
BstDSI CCRYGG 2 cut(s) 156, 1228
BstEII GGTNACC 1 cut(s) 467
BstFNI CGCG 1 cut(s) 669
BstKTI GATC 3 cut(s) 10, 1204, 1249
BstMAI GTCTC 4 cut(s) 90, 515, 794, 1025
BstMBI GATC 3 cut(s) 7, 1201, 1246
BstMWI GCNNNNNNNGC 2 cut(s) 1250, 1388
BstNI CCWGG 3 cut(s) 479, 531, 759
BstNSI RCATGY 3 cut(s) 1040, 1442, 1513
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 3 cut(s) 477, 529, 757
BstSFI CTRYAG 1 cut(s) 789
BstSLI GKGCMC 1 cut(s) 1036
BstUI CGCG 1 cut(s) 669
BstV1I GCAGC 1 cut(s) 1258
BstV2I GAAGAC 1 cut(s) 968
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 1216
BsuRI GGCC 3 cut(s) 454, 832, 1066
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 2 cut(s) 156, 1228
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 3 cut(s) 60, 186, 1286
CaiI CAGNNNCTG 1 cut(s) 530
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 2 cut(s) 752, 830
ClaI ATCGAT 1 cut(s) 378
Csp6I GTAC 2 cut(s) 1130, 1263
CviAII CATG 7 cut(s) 157, 1037, 1048, 1180, 1229, 1439, 1510
CviQI GTAC 2 cut(s) 1130, 1263
DdeI CTNAG 9 cut(s) 45, 296, 561, 660, 689, 966, 1088, 1266, 1335
DpnI GATC 3 cut(s) 9, 1203, 1248
DpnII GATC 3 cut(s) 7, 1201, 1246
Eam1104I CTCTTC 2 cut(s) 710, 1281
EarI CTCTTC 2 cut(s) 710, 1281
EciI GGCGGA 2 cut(s) 10, 20
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 2 cut(s) 156, 1228
Eco24I GRGCYC 1 cut(s) 17
Eco31I GGTCTC 1 cut(s) 1025
Eco47I GGWCC 1 cut(s) 752
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 4 cut(s) 152, 1262, 1386, 1457
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 15
EcoO65I GGTNACC 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 1345
EcoRII CCWGG 3 cut(s) 477, 529, 757
EcoT14I CCWWGG 2 cut(s) 156, 1228
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 2 cut(s) 156, 1228
FaeI CATG 7 cut(s) 160, 1040, 1051, 1183, 1232, 1442, 1513
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 1 cut(s) 1012
FatI CATG 7 cut(s) 156, 1036, 1047, 1179, 1228, 1438, 1509
FauI CCCGC 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 1254, 1272
FokI GGATG 9 cut(s) 4, 353, 505, 611, 1028, 1148, 1271, 1313, 1471
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 2 cut(s) 1254, 1272
GluI GCNGC 2 cut(s) 1254, 1272
GsuI CTGGAG 1 cut(s) 825
HaeIII GGCC 3 cut(s) 454, 832, 1066
HapII CCGG 2 cut(s) 451, 1133
Hin1II CATG 7 cut(s) 160, 1040, 1051, 1183, 1232, 1442, 1513
HinfI GANTC 4 cut(s) 443, 938, 964, 1164
HpaII CCGG 2 cut(s) 451, 1133
HphI GGTGA 5 cut(s) 194, 479, 545, 869, 983
Hpy166II GTNNAC 3 cut(s) 364, 1034, 1130
Hpy188I TCNGA 5 cut(s) 12, 269, 663, 1005, 1089
Hpy188III TCNNGA 6 cut(s) 32, 554, 1205, 1375, 1456, 1527
Hpy8I GTNNAC 3 cut(s) 364, 1034, 1130
HpyAV CCTTC 7 cut(s) 43, 380, 386, 457, 874, 1410, 1481
HpyCH4III ACNGT 4 cut(s) 190, 223, 542, 1420
HpyCH4IV ACGT 1 cut(s) 592
HpyCH4V TGCA 9 cut(s) 65, 145, 228, 419, 782, 1034, 1190, 1284, 1382
HpyF10VI GCNNNNNNNGC 2 cut(s) 1250, 1388
HpyF3I CTNAG 9 cut(s) 45, 296, 561, 660, 689, 966, 1088, 1266, 1335
HpySE526I ACGT 1 cut(s) 592
Hsp92II CATG 7 cut(s) 160, 1040, 1051, 1183, 1232, 1442, 1513
Kzo9I GATC 3 cut(s) 7, 1201, 1246
LguI GCTCTTC 2 cut(s) 710, 1281
LmnI GCTCC 5 cut(s) 73, 556, 806, 952, 1052
Lsp1109I GCAGC 1 cut(s) 1258
LweI GCATC 4 cut(s) 406, 483, 1293, 1321
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 8 cut(s) 182, 397, 467, 681, 1357, 1424, 1495, 1532
MalI GATC 3 cut(s) 9, 1203, 1248
MbiI CCGCTC 1 cut(s) 1256
MboI GATC 3 cut(s) 7, 1201, 1246
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 17, 1036
MluCI AATT 6 cut(s) 66, 127, 387, 608, 1326, 1345
MlyI GAGTC 1 cut(s) 958
MmeI TCCRAC 2 cut(s) 616, 1123
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 165
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 2 cut(s) 719, 1233
MspI CCGG 2 cut(s) 451, 1133
MspR9I CCNGG 3 cut(s) 479, 531, 759
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 2 cut(s) 809, 910
MvaI CCWGG 3 cut(s) 479, 531, 759
MvnI CGCG 1 cut(s) 669
MwoI GCNNNNNNNGC 2 cut(s) 1250, 1388
NcoI CCATGG 2 cut(s) 156, 1228
NdeII GATC 3 cut(s) 7, 1201, 1246
NlaIII CATG 7 cut(s) 160, 1040, 1051, 1183, 1232, 1442, 1513
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 5 cut(s) 182, 467, 1357, 1424, 1495
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 3 cut(s) 1040, 1442, 1513
PciI ACATGT 3 cut(s) 1036, 1438, 1509
PciSI GCTCTTC 2 cut(s) 710, 1281
PctI GAATGC 2 cut(s) 809, 910
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 3 cut(s) 443, 938, 1164
PkrI GCNGC 2 cut(s) 1255, 1273
PleI GAGTC 1 cut(s) 958
PpsI GAGTC 1 cut(s) 958
PscI ACATGT 3 cut(s) 1036, 1438, 1509
PshBI ATTAAT 1 cut(s) 825
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 3 cut(s) 477, 529, 757
PspEI GGTNACC 1 cut(s) 467
PspGI CCWGG 3 cut(s) 477, 529, 757
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 2 cut(s) 752, 830
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 2 cut(s) 1131, 1264
RsaNI GTAC 2 cut(s) 1130, 1263
RseI CAYNNNNRTG 2 cut(s) 719, 1233
SacI GAGCTC 1 cut(s) 17
SapI GCTCTTC 2 cut(s) 710, 1281
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 2 cut(s) 1254, 1272
Sau3AI GATC 3 cut(s) 7, 1201, 1246
Sau96I GGNCC 2 cut(s) 752, 830
SchI GAGTC 1 cut(s) 958
ScrFI CCNGG 3 cut(s) 479, 531, 759
SduI GDGCHC 2 cut(s) 17, 1036
SfaNI GCATC 4 cut(s) 406, 483, 1293, 1321
SfcI CTRYAG 1 cut(s) 789
SinI GGWCC 1 cut(s) 752
SmiMI CAYNNNNRTG 2 cut(s) 719, 1233
SmlI CTYRAG 2 cut(s) 874, 1022
SmoI CTYRAG 2 cut(s) 874, 1022
Sse9I AATT 6 cut(s) 66, 127, 387, 608, 1326, 1345
SsiI CCGC 4 cut(s) 5, 21, 358, 1254
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 3 cut(s) 477, 529, 757
StyI CCWWGG 2 cut(s) 156, 1228
TaaI ACNGT 4 cut(s) 190, 223, 542, 1420
TaiI ACGT 1 cut(s) 595
TaqI TCGA 3 cut(s) 94, 378, 1206
TasI AATT 6 cut(s) 66, 127, 387, 608, 1326, 1345
TatI WGTACW 1 cut(s) 1262
TauI GCSGC 1 cut(s) 1256
TfiI GAWTC 3 cut(s) 443, 938, 1164
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 3 cut(s) 67, 193, 1286
TseFI GTSAC 5 cut(s) 182, 467, 1357, 1424, 1495
TseI GCWGC 1 cut(s) 1271
Tsp45I GTSAC 5 cut(s) 182, 467, 1357, 1424, 1495
TspDTI ATGAA 7 cut(s) 123, 258, 287, 317, 1152, 1196, 1380
TspGWI ACGGA 1 cut(s) 1277
TspRI CASTG 3 cut(s) 67, 193, 1286
VneI GTGCAC 1 cut(s) 1032
VpaK11BI GGWCC 1 cut(s) 752
VspI ATTAAT 1 cut(s) 825
XapI RAATTY 1 cut(s) 1345
XceI RCATGY 3 cut(s) 1040, 1442, 1513
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmnI GAANNNNTTC 1 cut(s) 165
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.