FvH4_5g05770

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013824
Physical Location & Seq
Forward (+)
3413641 .. 3417728
4088 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05770.t2

Sequence Viewer

Length: 2445 bp
ATGGCGGATCCGAGCTCGTCCGGCGCTAATTCGGCCCAGCCCTCTTCTACTGACCCGAAAGCTAAGAAGGATTTTTCCACGGCGATTTTGGAGCGGAAGAAGTCTCCTAATCGGCTTGTAGTTGATGAAGCTATCAATGATGATAATTCTGTGGTGTCCTTGCACCCTGCTACCATGGAAAAACTTCAGTTTTTCCGGGGAGACACTGTGCTAATAAAGGGGAAGAAACGGAAAGATACAGTTTGTATTGTTCTTGCTGATGAACAATGTGACGAACCAAGGATCAGAATGAACAAAGTTGTAAGGGCTAATCTTAGGGTGCGTCTTGGAGATGTTGTGTCAGTTCACACTTGTCCGGATGTCAAGTATGGGAAGCGGGTTCACATCCTTCCAATTGATGATACAATTGAAGGCGTATCTGGAAACTTGTTTGATGCATATTTGAAGCCATACTTCTTGGAATCTTACCGGCCTGTGAGGAAGGGTGACCTTTTCCTGGTCAGGGGTGGGATGCGAAGTGTTGAGTTCAAGGTCATTGACACAGACCCTGGTGAATACTGTGTTGTTGCTCCCGATACTGAGATTTTCTGTGAGGGAGAACCTGTGAAGCGTGAGGATGAGGAAAAATTGAATGAGGTGGGTTATGATGATGTTGGAGGTGTTAGGAAACAAATGGCTCAGATACGTGAATTAGTAGAGTTACCTCTTAGACACCCACAGCTTTTCAAGTCCATTGGAGTGAAACCACCAAAAGGTATTCTGCTTTATGGACCACCGGGGTCTGGGAAAACTCTGATTGCAAGGGCTGTAGCTAATGAAACTGGAGCATTCTTCTTTTTGATTAATGGGCCTGAAATAATGTCAAAGTTAGCTGGTGAGAGTGAAAGCAATCTGAGGAAGGCATTTGAAGAAGCTGAAAAGAATGCCCCATCAATCATTTTTATTGATGAGTTAGATTCCATTGCTCCAAAGAGGGAGAAGACTCAGGGTGAAGTGGAAAGGCGTATCGTTTCGCAACTTCTGACTTTGATGGATGGCCTCAAGTCCCGTGCACATGTCATTGTCATGGCAGCAACAAATAGGCCAAACAGCATTGACCCGGCTCTGAGGAGGTTTGGAAGATTTGATAGAGAGATTGACATTGGTGTACCAGATGAAATTGGAAGATTGGAAGTTTTGAGAATCCATACAAAGAACATGAAACTTGCAGAAGATGTTGATCTCGAACGAGTTTCAAAGGACACCCATGGTTATGTCGGCGCTGATCTTGCCGCACTCTGTACTGAGGCTGCACTTCAGTGTATCCGTGAGAAAATGGATGTTATTGACTTAGAAGATGAGGAAATTGATGCCGAGGTGCTGAATTCTATGGCTGTGACCAATGAACACTTCCAGACTGCATTGGGTGCTTCAAATCCTTCAGCATTGCGTGAAACTGTTGTGGAGGTACCTAATGTCTCATGGGATGCAATTGGAGGATTGGATAATGTCAAAAGAGAACTTCAAGAGACTGTTCAATACCCAGTGGAGCATCCAGAGAAGTTTGAGAAATTTGGCATGTCACCTTCTAAAGGTGTTCTCTTTTATGGGCCACCAGGCTGTGGTAAAACTCTTCTTGCAAAGGCAATTGCCAATGAATGCCAGGCTAATTTCATTAGTATCAAAGGCCCTGAGTTGCTAACAATGTGGTTTGGAGAAAGTGAGGCAAATGTCCGTGAGATATTTGACAAAGCTCGCCAATCTGCTCCTTGTGTTCTTTTCTTTGATGAGCTGGACTCAATTGCAACTCAGCGTGGAGGTTCTGTAGGAGATGCTGGTGGTGCAGCAGATAGAGTCTTGAACCAACTTCTAACTGAAATGGATGGAATGAGTGCTAAGAAAACAGTTTTTATCATTGGAGCAACAAATAGGCCAGACATTATTGACCCAGCATTGCTCAGGCCTGGACGGCTTGACCAGCTCATCTATATCCCACTCCCAGATGAAGCTTCCCGTCTTCAGATTTTCAAAGCATGTTTGAGGAAGTCACCCGTCAATAGGGATGTTGACCTTGCAGTTCTTGCACGTCATACCCACGGTTTCAGTGGTGCTGATATCACTGAGATATGCCAGCGTGCCTGCAAATATGCCATCAGAGAAAATATTGAGAAGGATATTGAAAAGGAGAGAAGAAAGGCGGAGAATCCAGAAGCAATGGAGGAGGATGACATTGATGAGGTTCCAGAAATAAAGCCAGCACACTTTGAGGAGTCTATGAAATTTGCCCGCAGAAGTGTTAGTGATGCAGACATCAGGAAGTACCAGGCATTCGCTCAGACTCTGCAACAATCTCGTGGGATTGGGTCTGATTTCCGGTTCCCCCAAACAGGCAACACTGCAGGAGCAGCTGCAGACCCCTTTGCTTCAGCTTCAGCTGCTGCAGACGACGATGATCTGTACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

815

Amino Acids

90.02

Weight (kDa)

5.17

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 61 - 142 2.2e-23 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 162 - 226 1.4e-11 Cell division protein 48 (CDC48), domain 2
AAA PF00004 276 - 405 1.4e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 428 - 471 1.3e-15 AAA+ lid domain
AAA PF00004 549 - 682 4.1e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 704 - 744 1.5e-13 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1447
AccB1I GGYRCC 1 cut(s) 1447
AccB7I CCANNNNNTGG 1 cut(s) 1601
AccBSI CCGCTC 1 cut(s) 94
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 6 cut(s) 5, 94, 376, 1272, 2177, 2266
AclWI GGATC 3 cut(s) 2, 15, 290
AcsI RAATTY 3 cut(s) 1363, 1550, 2258
AcuI CTGAAG 6 cut(s) 170, 1280, 1404, 1982, 2388, 2394
AfaI GTAC 5 cut(s) 1149, 1282, 1449, 2300, 2438
AfiI CCNNNNNNNGG 8 cut(s) 496, 502, 752, 782, 1571, 1601, 2037, 2366
AflIII ACRYGT 1 cut(s) 1054
AjiI CACGTC 1 cut(s) 2066
AjnI CCWGG 6 cut(s) 495, 547, 1594, 1641, 1942, 2301
AjuI GAANNNNNNNTTGG 2 cut(s) 1373, 1405
AleI CACNNNNGTG 1 cut(s) 1297
Alw21I GWGCWC 2 cut(s) 17, 1054
Alw26I GTCTC 4 cut(s) 108, 195, 1462, 1502
Alw44I GTGCAC 1 cut(s) 1050
AlwI GGATC 3 cut(s) 2, 15, 290
AlwNI CAGNNNCTG 3 cut(s) 548, 2320, 2417
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 9 cut(s) 33, 470, 848, 1036, 1082, 1589, 1666, 1910, 1940
ApaLI GTGCAC 1 cut(s) 1050
ApeKI GCWGC 7 cut(s) 1070, 1289, 1823, 2384, 2387, 2414, 2417
ApoI RAATTY 3 cut(s) 1363, 1550, 2258
AseI ATTAAT 1 cut(s) 843
Asp700I GAANNNNTTC 1 cut(s) 183
Asp718I GGTACC 1 cut(s) 1447
AspLEI GCGC 2 cut(s) 26, 1262
AspS9I GGNCC 5 cut(s) 34, 770, 848, 1589, 1667
AsuC2I CCSGG 3 cut(s) 197, 777, 1100
AsuHPI GGTGA 6 cut(s) 497, 563, 887, 1001, 1554, 2019
AvaII GGWCC 1 cut(s) 770
BaeGI GKGCMC 1 cut(s) 1054
BamHI GGATCC 1 cut(s) 7
BanI GGYRCC 1 cut(s) 1447
BanII GRGCYC 1 cut(s) 17
BauI CACGAG 1 cut(s) 2331
BbsI GAAGAC 2 cut(s) 986, 1988
Bbv12I GWGCWC 2 cut(s) 17, 1054
BbvI GCAGC 7 cut(s) 1082, 1276, 1835, 2374, 2396, 2401, 2404
BccI CCATC 5 cut(s) 937, 1024, 1028, 1856, 2138
BceAI ACGGC 2 cut(s) 96, 1964
BcgI CGANNNNNNTGC 2 cut(s) 2312, 2346
BciT130I CCWGG 6 cut(s) 497, 549, 1596, 1643, 1944, 2303
BciVI GTATCC 1 cut(s) 1313
BcnI CCSGG 3 cut(s) 197, 777, 1100
BcoDI GTCTC 4 cut(s) 108, 195, 1462, 1502
BfmI CTRYAG 5 cut(s) 807, 1803, 2376, 2388, 2418
BfoI RGCGCY 2 cut(s) 27, 1263
BfuI GTATCC 1 cut(s) 1313
BglI GCCNNNNNGGC 1 cut(s) 1948
BisI GCNGC 8 cut(s) 1071, 1272, 1290, 1824, 2385, 2388, 2415, 2418
BlsI GCNGC 8 cut(s) 1072, 1273, 1291, 1825, 2386, 2389, 2416, 2419
Bme1390I CCNGG 9 cut(s) 197, 497, 549, 777, 1100, 1596, 1643, 1944, 2303
Bme18I GGWCC 1 cut(s) 770
BmgBI CACGTC 1 cut(s) 2066
BmgT120I GGNCC 5 cut(s) 34, 770, 848, 1589, 1667
BmiI GGNNCC 4 cut(s) 9, 1449, 2220, 2357
BmrFI CCNGG 9 cut(s) 197, 497, 549, 777, 1100, 1596, 1643, 1944, 2303
BmrI ACTGGG 1 cut(s) 1517
BmsI GCATC 7 cut(s) 424, 501, 1339, 1456, 1540, 1801, 2272
BmuI ACTGGG 1 cut(s) 1517
BpiI GAAGAC 2 cut(s) 986, 1988
BplI GAGNNNNNCTC 2 cut(s) 1760, 1792
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 1937
BpuEI CTTGAG 1 cut(s) 1025
BpuMI CCSGG 3 cut(s) 197, 777, 1100
BsaAI YACGTR 1 cut(s) 686
BsaBI GATNNNNATC 1 cut(s) 1218
BsaJI CCNNGG 9 cut(s) 78, 174, 196, 278, 547, 776, 1246, 1353, 2074
BsaWI WCCGGW 2 cut(s) 355, 2352
BsaXI ACNNNNNCTCC 8 cut(s) 192, 222, 321, 351, 588, 618, 1788, 1818
Bsc4I CCNNNNNNNGG 8 cut(s) 496, 502, 752, 782, 1571, 1601, 2037, 2366
Bse118I RCCGGY 1 cut(s) 468
Bse1I ACTGG 2 cut(s) 826, 1523
Bse3DI GCAATG 4 cut(s) 960, 1424, 1931, 2199
Bse8I GATNNNNATC 1 cut(s) 1218
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 6 cut(s) 497, 549, 1596, 1643, 1944, 2303
BseDI CCNNGG 9 cut(s) 78, 174, 196, 278, 547, 776, 1246, 1353, 2074
BseJI GATNNNNATC 1 cut(s) 1218
BseLI CCNNNNNNNGG 8 cut(s) 496, 502, 752, 782, 1571, 1601, 2037, 2366
BseMI GCAATG 4 cut(s) 960, 1424, 1931, 2199
BseNI ACTGG 2 cut(s) 826, 1523
BseRI GAGGAG 3 cut(s) 1123, 2213, 2261
BseSI GKGCMC 1 cut(s) 1054
BseXI GCAGC 7 cut(s) 1082, 1276, 1835, 2374, 2396, 2401, 2404
BseYI CCCAGC 2 cut(s) 36, 1927
BsgI GTGCAG 2 cut(s) 1275, 1842
BshFI GGCC 9 cut(s) 35, 472, 850, 1038, 1084, 1591, 1668, 1912, 1942
BshNI GGYRCC 1 cut(s) 1447
BsiHKAI GWGCWC 2 cut(s) 17, 1054
BsiSI CCGG 7 cut(s) 21, 196, 356, 469, 776, 1100, 2353
BslFI GGGAC 1 cut(s) 1030
BslI CCNNNNNNNGG 8 cut(s) 496, 502, 752, 782, 1571, 1601, 2037, 2366
BsmAI GTCTC 4 cut(s) 108, 195, 1462, 1502
BsmFI GGGAC 1 cut(s) 1030
BsmI GAATGC 4 cut(s) 827, 928, 1643, 2306
BsnI GGCC 9 cut(s) 35, 472, 850, 1038, 1084, 1591, 1668, 1912, 1942
Bsp1286I GDGCHC 2 cut(s) 17, 1054
Bsp13I TCCGGA 1 cut(s) 355
Bsp1407I TGTACA 1 cut(s) 2436
Bsp143I GATC 5 cut(s) 7, 282, 1219, 1264, 2431
Bsp19I CCATGG 2 cut(s) 174, 1246
BspACI CCGC 6 cut(s) 5, 94, 376, 1272, 2177, 2266
BspANI GGCC 9 cut(s) 35, 472, 850, 1038, 1084, 1591, 1668, 1912, 1942
BspEI TCCGGA 1 cut(s) 355
BspLI GGNNCC 4 cut(s) 9, 1449, 2220, 2357
BspMAI CTGCAG 3 cut(s) 2380, 2392, 2422
BspPI GGATC 3 cut(s) 2, 15, 290
BspT107I GGYRCC 1 cut(s) 1447
BsrBI CCGCTC 1 cut(s) 94
BsrDI GCAATG 4 cut(s) 960, 1424, 1931, 2199
BsrFI RCCGGY 1 cut(s) 468
BsrGI TGTACA 1 cut(s) 2436
BsrI ACTGG 2 cut(s) 826, 1523
BssAI RCCGGY 1 cut(s) 468
BssECI CCNNGG 9 cut(s) 78, 174, 196, 278, 547, 776, 1246, 1353, 2074
BssMI GATC 5 cut(s) 7, 282, 1219, 1264, 2431
BssSI CACGAG 1 cut(s) 2331
BssT1I CCWWGG 3 cut(s) 174, 278, 1246
Bst2BI CACGAG 1 cut(s) 2331
Bst2UI CCWGG 6 cut(s) 497, 549, 1596, 1643, 1944, 2303
Bst4CI ACNGT 7 cut(s) 208, 241, 560, 1438, 1513, 1885, 2078
Bst6I CTCTTC 2 cut(s) 49, 1617
BstAPI GCANNNNNTGC 2 cut(s) 1406, 2060
BstAUI TGTACA 1 cut(s) 2436
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 6 cut(s) 1735, 2111, 2115, 2119, 2235, 2266
BstDSI CCRYGG 4 cut(s) 78, 174, 1246, 2074
BstEII GGTNACC 1 cut(s) 485
BstENI CCTNNNNNAGG 1 cut(s) 1569
BstH2I RGCGCY 2 cut(s) 27, 1263
BstHHI GCGC 2 cut(s) 26, 1262
BstKTI GATC 5 cut(s) 10, 285, 1222, 1267, 2434
BstMAI GTCTC 4 cut(s) 108, 195, 1462, 1502
BstMBI GATC 5 cut(s) 7, 282, 1219, 1264, 2431
BstNI CCWGG 6 cut(s) 497, 549, 1596, 1643, 1944, 2303
BstNSI RCATGY 3 cut(s) 1058, 1561, 2016
BstPI GGTNACC 1 cut(s) 485
BstSCI CCNGG 9 cut(s) 195, 495, 547, 775, 1098, 1594, 1641, 1942, 2301
BstSFI CTRYAG 5 cut(s) 807, 1803, 2376, 2388, 2418
BstSLI GKGCMC 1 cut(s) 1054
BstV1I GCAGC 7 cut(s) 1082, 1276, 1835, 2374, 2396, 2401, 2404
BstV2I GAAGAC 2 cut(s) 986, 1988
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuI GTATCC 1 cut(s) 1313
BsuRI GGCC 9 cut(s) 35, 472, 850, 1038, 1084, 1591, 1668, 1912, 1942
BtgI CCRYGG 4 cut(s) 78, 174, 1246, 2074
BtrI CACGTC 1 cut(s) 2066
BtsI GCAGTG 1 cut(s) 2373
BtsIMutI CAGTG 6 cut(s) 204, 1304, 1530, 2089, 2097, 2373
Cac8I GCNNGC 6 cut(s) 1735, 2111, 2115, 2119, 2235, 2266
CaiI CAGNNNCTG 3 cut(s) 548, 2320, 2417
CfoI GCGC 2 cut(s) 26, 1262
Cfr10I RCCGGY 1 cut(s) 468
Cfr13I GGNCC 5 cut(s) 34, 770, 848, 1589, 1667
CseI GACGC 1 cut(s) 311
Csp6I GTAC 5 cut(s) 1148, 1281, 1448, 2299, 2437
CviAII CATG 8 cut(s) 175, 1055, 1066, 1198, 1247, 1461, 1558, 2013
CviQI GTAC 5 cut(s) 1148, 1281, 1448, 2299, 2437
DpnI GATC 5 cut(s) 9, 284, 1221, 1266, 2433
DpnII GATC 5 cut(s) 7, 282, 1219, 1264, 2431
Eam1104I CTCTTC 2 cut(s) 49, 1617
EarI CTCTTC 2 cut(s) 49, 1617
EciI GGCGGA 2 cut(s) 20, 2192
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 3 cut(s) 174, 278, 1246
Eco147I AGGCCT 1 cut(s) 1942
Eco24I GRGCYC 1 cut(s) 17
Eco32I GATATC 1 cut(s) 2095
Eco47I GGWCC 1 cut(s) 770
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 6 cut(s) 170, 1280, 1404, 1982, 2388, 2394
Eco91I GGTNACC 1 cut(s) 485
EcoICRI GAGCTC 1 cut(s) 15
EcoNI CCTNNNNNAGG 1 cut(s) 1569
EcoO109I RGGNCCY 1 cut(s) 1667
EcoO65I GGTNACC 1 cut(s) 485
EcoRI GAATTC 1 cut(s) 1363
EcoRII CCWGG 6 cut(s) 495, 547, 1594, 1641, 1942, 2301
EcoRV GATATC 1 cut(s) 2095
EcoT14I CCWWGG 3 cut(s) 174, 278, 1246
EcoT22I ATGCAT 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 3 cut(s) 174, 278, 1246
FaeI CATG 8 cut(s) 178, 1058, 1069, 1201, 1250, 1464, 1561, 2016
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 1 cut(s) 1030
FatI CATG 8 cut(s) 174, 1054, 1065, 1197, 1246, 1460, 1557, 2012
FauI CCCGC 2 cut(s) 369, 2273
Fnu4HI GCNGC 8 cut(s) 1071, 1272, 1290, 1824, 2385, 2388, 2415, 2418
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 8 cut(s) 1071, 1272, 1290, 1824, 2385, 2388, 2415, 2418
GlaI GCGC 2 cut(s) 25, 1261
GluI GCNGC 8 cut(s) 1071, 1272, 1290, 1824, 2385, 2388, 2415, 2418
GsaI CCCAGC 2 cut(s) 40, 1931
GsuI CTGGAG 1 cut(s) 843
HaeII RGCGCY 2 cut(s) 27, 1263
HaeIII GGCC 9 cut(s) 35, 472, 850, 1038, 1084, 1591, 1668, 1912, 1942
HapII CCGG 7 cut(s) 21, 196, 356, 469, 776, 1100, 2353
HgaI GACGC 1 cut(s) 311
HhaI GCGC 2 cut(s) 26, 1262
Hin1II CATG 8 cut(s) 178, 1058, 1069, 1201, 1250, 1464, 1561, 2016
Hin6I GCGC 2 cut(s) 24, 1260
HinP1I GCGC 2 cut(s) 24, 1260
HincII GTYRAC 1 cut(s) 2047
HindII GTYRAC 1 cut(s) 2047
HindIII AAGCTT 1 cut(s) 1986
HinfI GANTC 9 cut(s) 461, 956, 982, 1182, 1775, 1833, 2182, 2249, 2317
HpaII CCGG 7 cut(s) 21, 196, 356, 469, 776, 1100, 2353
HphI GGTGA 6 cut(s) 497, 563, 887, 1001, 1554, 2019
Hpy166II GTNNAC 5 cut(s) 346, 382, 1052, 1148, 2047
Hpy8I GTNNAC 5 cut(s) 346, 382, 1052, 1148, 2047
Hpy99I CGWCG 1 cut(s) 2429
HpyAV CCTTC 8 cut(s) 61, 398, 404, 475, 892, 1428, 1575, 2143
HpyCH4III ACNGT 7 cut(s) 208, 241, 560, 1438, 1513, 1885, 2078
HpyCH4IV ACGT 2 cut(s) 685, 2065
HpySE526I ACGT 2 cut(s) 685, 2065
Hsp92II CATG 8 cut(s) 178, 1058, 1069, 1201, 1250, 1464, 1561, 2016
HspAI GCGC 2 cut(s) 24, 1260
Kpn2I TCCGGA 1 cut(s) 355
KpnI GGTACC 1 cut(s) 1451
Kzo9I GATC 5 cut(s) 7, 282, 1219, 1264, 2431
LmnI GCTCC 8 cut(s) 91, 574, 824, 970, 1528, 1750, 1898, 2381
Lsp1109I GCAGC 7 cut(s) 1082, 1276, 1835, 2374, 2396, 2401, 2404
LweI GCATC 7 cut(s) 424, 501, 1339, 1456, 1540, 1801, 2272
MaeII ACGT 2 cut(s) 685, 2065
MaeIII GTNAC 6 cut(s) 269, 485, 699, 1375, 1560, 2025
MalI GATC 5 cut(s) 9, 284, 1221, 1266, 2433
MbiI CCGCTC 1 cut(s) 94
MboI GATC 5 cut(s) 7, 282, 1219, 1264, 2431
MfeI CAATTG 5 cut(s) 393, 405, 1470, 1626, 1779
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 17, 1054
MlyI GAGTC 5 cut(s) 976, 1769, 1842, 2258, 2311
MmeI TCCRAC 1 cut(s) 634
Mph1103I ATGCAT 1 cut(s) 439
MroI TCCGGA 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 183
MseI TTAA 1 cut(s) 843
MslI CAYNNNNRTG 4 cut(s) 737, 1064, 1251, 1297
MspA1I CMGCKG 3 cut(s) 2387, 2414, 2442
MspI CCGG 7 cut(s) 21, 196, 356, 469, 776, 1100, 2353
MspR9I CCNGG 9 cut(s) 197, 497, 549, 777, 1100, 1596, 1643, 1944, 2303
MunI CAATTG 5 cut(s) 393, 405, 1470, 1626, 1779
Mva1269I GAATGC 4 cut(s) 827, 928, 1643, 2306
MvaI CCWGG 6 cut(s) 497, 549, 1596, 1643, 1944, 2303
NciI CCSGG 3 cut(s) 197, 777, 1100
NcoI CCATGG 2 cut(s) 174, 1246
NdeII GATC 5 cut(s) 7, 282, 1219, 1264, 2431
NlaIII CATG 8 cut(s) 178, 1058, 1069, 1201, 1250, 1464, 1561, 2016
NlaIV GGNNCC 4 cut(s) 9, 1449, 2220, 2357
NmeAIII GCCGAG 1 cut(s) 1378
NmuCI GTSAC 5 cut(s) 269, 485, 1375, 1560, 2025
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 3 cut(s) 1058, 1561, 2016
OliI CACNNNNGTG 1 cut(s) 1297
PceI AGGCCT 1 cut(s) 1942
PciI ACATGT 1 cut(s) 1054
PctI GAATGC 4 cut(s) 827, 928, 1643, 2306
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 4 cut(s) 461, 956, 1182, 2182
PflMI CCANNNNNTGG 1 cut(s) 1601
PkrI GCNGC 8 cut(s) 1072, 1273, 1291, 1825, 2386, 2389, 2416, 2419
PleI GAGTC 5 cut(s) 976, 1769, 1841, 2257, 2311
PpsI GAGTC 5 cut(s) 976, 1769, 1841, 2257, 2311
Ppu21I YACGTR 1 cut(s) 686
PscI ACATGT 1 cut(s) 1054
PshBI ATTAAT 1 cut(s) 843
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 6 cut(s) 495, 547, 1594, 1641, 1942, 2301
PspEI GGTNACC 1 cut(s) 485
PspFI CCCAGC 2 cut(s) 36, 1927
PspGI CCWGG 6 cut(s) 495, 547, 1594, 1641, 1942, 2301
PspN4I GGNNCC 4 cut(s) 9, 1449, 2220, 2357
PspPI GGNCC 5 cut(s) 34, 770, 848, 1589, 1667
PstI CTGCAG 3 cut(s) 2380, 2392, 2422
PstNI CAGNNNCTG 3 cut(s) 548, 2320, 2417
PsuI RGATCY 1 cut(s) 7
PvuII CAGCTG 3 cut(s) 2387, 2414, 2442
RsaI GTAC 5 cut(s) 1149, 1282, 1449, 2300, 2438
RsaNI GTAC 5 cut(s) 1148, 1281, 1448, 2299, 2437
RseI CAYNNNNRTG 4 cut(s) 737, 1064, 1251, 1297
SacI GAGCTC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 843
SatI GCNGC 8 cut(s) 1071, 1272, 1290, 1824, 2385, 2388, 2415, 2418
Sau3AI GATC 5 cut(s) 7, 282, 1219, 1264, 2431
Sau96I GGNCC 5 cut(s) 34, 770, 848, 1589, 1667
SchI GAGTC 5 cut(s) 976, 1769, 1842, 2258, 2311
ScrFI CCNGG 9 cut(s) 197, 497, 549, 777, 1100, 1596, 1643, 1944, 2303
SduI GDGCHC 2 cut(s) 17, 1054
SfaNI GCATC 7 cut(s) 424, 501, 1339, 1456, 1540, 1801, 2272
SfcI CTRYAG 5 cut(s) 807, 1803, 2376, 2388, 2418
SinI GGWCC 1 cut(s) 770
SmiMI CAYNNNNRTG 4 cut(s) 737, 1064, 1251, 1297
SmlI CTYRAG 1 cut(s) 1040
SmoI CTYRAG 1 cut(s) 1040
SseBI AGGCCT 1 cut(s) 1942
SsiI CCGC 6 cut(s) 5, 94, 376, 1272, 2177, 2266
SspI AATATT 1 cut(s) 2143
SstI GAGCTC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 1942
StyD4I CCNGG 9 cut(s) 195, 495, 547, 775, 1098, 1594, 1641, 1942, 2301
StyI CCWWGG 3 cut(s) 174, 278, 1246
TaaI ACNGT 7 cut(s) 208, 241, 560, 1438, 1513, 1885, 2078
TaiI ACGT 2 cut(s) 688, 2068
TaqI TCGA 1 cut(s) 1224
TatI WGTACW 2 cut(s) 1280, 2436
TauI GCSGC 1 cut(s) 1274
TfiI GAWTC 4 cut(s) 461, 956, 1182, 2182
Tru1I TTAA 1 cut(s) 843
Tru9I TTAA 1 cut(s) 843
TscAI CASTG 6 cut(s) 211, 1304, 1530, 2089, 2104, 2380
TseFI GTSAC 5 cut(s) 269, 485, 1375, 1560, 2025
TseI GCWGC 7 cut(s) 1070, 1289, 1823, 2384, 2387, 2414, 2417
Tsp45I GTSAC 5 cut(s) 269, 485, 1375, 1560, 2025
TspGWI ACGGA 3 cut(s) 244, 1295, 1703
TspRI CASTG 6 cut(s) 211, 1304, 1530, 2089, 2104, 2380
Van91I CCANNNNNTGG 1 cut(s) 1601
VneI GTGCAC 1 cut(s) 1050
VpaK11BI GGWCC 1 cut(s) 770
VspI ATTAAT 1 cut(s) 843
XagI CCTNNNNNAGG 1 cut(s) 1569
XapI RAATTY 3 cut(s) 1363, 1550, 2258
XceI RCATGY 3 cut(s) 1058, 1561, 2016
XcmI CCANNNNNNNNNTGG 2 cut(s) 85, 2081
XmnI GAANNNNTTC 1 cut(s) 183
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.