Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005666

Physical Location & Seq

Genomic Coordinates Forward (+)

931762 .. 937702

Gene Structure 5941 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G03650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGGTGGTGATTCACGGAGTGTCTCTTACTCCACGCTTCACTCTTCCTTCTCGACCTCTCAACACTGGCTTTAATGCCGGCAATTCCACCCTCTCTTTCTTCTTCAAAAAGCACCCTCTCTCTCGGAAGATCTTTGCTGGGAAACAATCTGCGGAGTTTGATTCTTCGTCACAAGCTATCTCTGCTTCTGAGAAAGTCTTAGTACCTGATAATCTTGATGATGATCCCAGAGGTTTTTCACAGATATTTGATCTAGAAAGTCAAACAATGGAATATACTGAGGCAGTAAGAACAGAAGACCAAACAATGAACGTTGTCAAGGAGAGAGGGGTGAAACCAAGAATAGTTCCCCCACCGGGTGATGGCAAGAAAATTTATGAGATAGACCCCATGTTACGAACTTACAACAATCATCTTGATTACCGTTATGGACAGTATAAAAGATTGCGTGAGGAAATAGACAAGTATGAGGGTGGTCTTGAGGCATTCTCTCGTGGCTATGAAAAGTTAGGATTTTCGCGCAGTGATGCCGGTATAACTTATAGAGAGTGGGCGCCTGGAGCTAAGGCTGCATCACTTATCGGAGATTTCAACAACTGGAATTCTAATGCAGATATCATGACTCGGAATGAATTTGGTGTTTGGGAGATCTTTTTGCCCAACAACACTGATGGTTCGCCTGCAATTCCTCATGGCTCACGTGTAAAGATTCGTATGGATACTCCATCTGGCATTAAAGACTCAATTCCTGCTTGGATCAAGTTCTCGGTGCAAGCTCCAGGTGAAATCCCATTCAATGGCATATACTATGATCCTCCAGAAGAGGAGAAGTATGTATTCAAACATCCTCAACCAAAGAGACCTAAGTCGCTAAGGATTTATGAAGCACATGTTGGCATGAGTAGCACGGAACCAATGGTCAATACGTATGCTAACTTTAGAGATGATGTTCTTCCCCGCATCAAAAAGCTTGGATATAATGCTGTTCAAATTATGGCCATACAAGAACATTCATATTATGCCAGCTTTGGGTACCATGTCACAAACTTTTTTGCCCCAAGCAGTCGCTGTGGGACCCCAGAGGAACTAAAATCACTGATAGATAGAGCTCACGAGTTAGGCCTGGTAGTTCTGATGGATATCGTTCATAGCCATGCTTCAAAAAACACATTGGATGGACTGAACATGTTTGATGGAACTGATGCTCACTATTTTCACTCTGGACCTCGGGGATACCATTGGATGTGGGATTCACGACTTTTCAATTATGGGAGCTGGGAAGTATTACGATATCTCCTTTCAAATGCACGGTGGTGGCTAGAAGAATACAAGTTTGATGGATTTAGATTTGATGGTGTAACCTCAATGATGTATACTCATCATGGACTCTCGGTTGGATTTACTGGGAACTACACCGAATACTTTGGATTGGAAACTGATGTGGATGCTGTGAATTATCTCATGCTGGTTAATGATATGATTCATGGGCTCTACCCTGAAGCGATTACCGTTGGTGAAGATGTTAGTGGTATGCCAACATTCTGTATTCCTGTCCAAGATGGTGGCGTTGGATTTGACTACCGTTTACACATGGCCATAGCTGATAAGTGGATAGAAATGCTCAAGAAGAGAGATGAAGACTGGCAAATGGGCGACATCATTTACACACTTACCAACAGAAGGTGGTCAGAGAAGTGTATCTCTTATGCTGAAAGTCACGATCAAGCTCTTGTTGGTGATAAAACAATTGCCTTCTGGTTAATGGACAAGGATATGTATGATTTCATGGCAGTAGACAGACCATCAACTCCTCTTATCGATAGAGGAATAGCTTTGCACAAAATGATTAGGCTTATAACTATGGGATTAGGCGGTGAAGGTTACTTAAATTTTATGGGAAACGAATTCGGACATCCAGAATGGATTGATTTTCCCAGAGGCGAGCAGCGTCTTTCTGATGGTAGCGTGATTCCTGGCAACAATTTCAGTTATGACAAATGCCGCCGCAGATTTGATCTTGGGGATGCAGATTATCTCAGATACCGCGGACTACAAGAATTTGATCAGGCAATGCAACATCTTGAAGAGAATTACGGTTTTATGACTTCGGAGCACCAATTCATATCACGAAAAGACGAAGCAGATAGAGTAATCGTATTCGAAAGAGGTGATCTCGTCTTTGTCTTTAACTTTCACTGGACCAGCAGCTACTTTGATTACCGCATTGGTTGCTCCAAGCCTGGAAAATATAAGATCGTATTGGACTCGGACGATCCTCTCTTTGGTGGATTCAATAGGCTCGATCGCAAGGCAGAGTACTTCACTTATGATGGCTTATACGACGAACGACCCTGCTCCTTCATGGTCTATGCACCGTGTAGAACCGCCGTGGTTTATGCTTTAGCAAACCACGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010021 GO:0010033 GO:0016740 GO:0016757 GO:0016758 GO:0019725 GO:0033500 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0042593 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071326 GO:0071329 GO:0071331 GO:0071332 GO:0071333 GO:0071478 GO:0071482 GO:0071704 GO:0104004 GO:1901135 GO:1901137 GO:1901576 GO:1901700 GO:1901701 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

805

Amino Acids

92.59

Weight (kDa)

5.54

Isoelectric Point (pI)

31.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	169 - 252	5.1e-19	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	318 - 576	3.2e-11	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	708 - 801	5.5e-21	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36390 AT5G03650
fragaria_vesca	FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120
malus_domestica	MD12G1020600.v1.1 MD14G1017700.v1.1
prunus_persica	Prupe.7G115400_v2.0.a1 Prupe.7G115400_v2.0.a1
pyrus_communis	pycom12g01930 pycom14g01610
rosa_chinensis	RchiOBHm_Chr3g0472421
rosa_laevigata	RLG00000024081
rosa_multiflora	Rmu_co8370739.1_g000001 Rmu_sc0001096.1_g000015
rosa_roxburghii	Rroxscaffold_6G00408930
rosa_rugosa	Rorug03G0124700 Rorug03G0124700 Rorug03G0124800 Rorug03G0124900
rosa_samantha	Rh3AG177100 Rh3BG203100 Rh3CG193400 Rh3CG200600 Rh3DG172000
rosa_wichuraiana	Rw3G016440

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1856
Acc65I	GGTACC	1 cut(s)	1032
AccB1I	GGYRCC	2 cut(s)	553, 1032
AccB7I	CCANNNNNTGG	1 cut(s)	797
AccI	GTMKAC	2 cut(s)	1373, 1794
AccII	CGCG	2 cut(s)	520, 2046
AciI	CCGC	9 cut(s)	152, 958, 1872, 2002, 2005, 2044, 2046, 2221, 2385
AclI	AACGTT	1 cut(s)	312
AclWI	GGATC	4 cut(s)	218, 764, 806, 2267
AcoI	YGGCCR	2 cut(s)	996, 1593
AcsI	RAATTY	6 cut(s)	372, 601, 632, 1888, 1904, 2057
AcuI	CTGAAG	1 cut(s)	1518
AcvI	CACGTG	1 cut(s)	701
AcyI	GRCGYC	1 cut(s)	554
AdeI	CACNNNGTG	2 cut(s)	19, 359
AfaI	GTAC	3 cut(s)	204, 1034, 2318
AfiI	CCNNNNNNNGG	6 cut(s)	356, 362, 797, 1029, 1680, 2282
AflIII	ACRYGT	3 cut(s)	700, 889, 1185
AjnI	CCWGG	5 cut(s)	556, 778, 1122, 1972, 2239
AjuI	GAANNNNNNNTTGG	2 cut(s)	876, 908
AleI	CACNNNNGTG	1 cut(s)	1312
Alw21I	GWGCWC	2 cut(s)	1111, 2115
Alw26I	GTCTC	2 cut(s)	27, 853
AlwI	GGATC	4 cut(s)	218, 764, 806, 2267
AlwNI	CAGNNNCTG	1 cut(s)	1068
Ama87I	CYCGRG	1 cut(s)	1227
AoxI	GGCC	3 cut(s)	996, 1120, 1593
ApeKI	GCWGC	3 cut(s)	569, 1945, 2205
ApoI	RAATTY	6 cut(s)	372, 601, 632, 1888, 1904, 2057
Asp718I	GGTACC	1 cut(s)	1032
AspLEI	GCGC	2 cut(s)	522, 556
AspS9I	GGNCC	3 cut(s)	1074, 1223, 2199
AsuC2I	CCSGG	1 cut(s)	357
AsuHPI	GGTGA	8 cut(s)	19, 343, 371, 794, 1526, 1748, 1886, 2180
AsuII	TTCGAA	1 cut(s)	2160
AvaI	CYCGRG	1 cut(s)	1227
AvaII	GGWCC	3 cut(s)	1074, 1223, 2199
BalI	TGGCCA	2 cut(s)	998, 1595
BanI	GGYRCC	2 cut(s)	553, 1032
BanII	GRGCYC	2 cut(s)	1111, 1491
BauI	CACGAG	2 cut(s)	492, 1112
BbrPI	CACGTG	1 cut(s)	701
BbsI	GAAGAC	2 cut(s)	303, 1644
Bbv12I	GWGCWC	2 cut(s)	1111, 2115
BbvI	GCAGC	3 cut(s)	556, 1957, 2217
BceAI	ACGGC	1 cut(s)	2372
BciT130I	CCWGG	5 cut(s)	558, 780, 1124, 1974, 2241
BciVI	GTATCC	2 cut(s)	712, 1226
BclI	TGATCA	1 cut(s)	2062
BcnI	CCSGG	1 cut(s)	357
BcoDI	GTCTC	2 cut(s)	27, 853
BfaI	CTAG	2 cut(s)	254, 1319
BfoI	RGCGCY	1 cut(s)	557
BfuI	GTATCC	2 cut(s)	712, 1226
BglII	AGATCT	2 cut(s)	129, 648
BisI	GCNGC	5 cut(s)	570, 1946, 2002, 2005, 2206
BlsI	GCNGC	5 cut(s)	571, 1947, 2003, 2006, 2207
BmcAI	AGTACT	1 cut(s)	2318
Bme1390I	CCNGG	6 cut(s)	357, 558, 780, 1124, 1974, 2241
Bme18I	GGWCC	3 cut(s)	1074, 1223, 2199
BmeT110I	CYCGRG	1 cut(s)	1227
BmgT120I	GGNCC	3 cut(s)	1074, 1223, 2199
BmiI	GGNNCC	5 cut(s)	555, 912, 1034, 1075, 1076
BmrFI	CCNGG	6 cut(s)	357, 558, 780, 1124, 1974, 2241
BmrI	ACTGGG	1 cut(s)	1413
BmsI	GCATC	6 cut(s)	517, 581, 969, 1192, 1435, 2014
BmuI	ACTGGG	1 cut(s)	1413
BoxI	GACNNNNGTC	1 cut(s)	865
BpiI	GAAGAC	2 cut(s)	303, 1644
BplI	GAGNNNNNCTC	4 cut(s)	473, 505, 2157, 2189
BpmI	CTGGAG	3 cut(s)	579, 762, 801
Bpu10I	CCTNAGC	2 cut(s)	564, 872
Bpu14I	TTCGAA	1 cut(s)	2160
BpuEI	CTTGAG	2 cut(s)	500, 1607
BpuMI	CCSGG	1 cut(s)	357
Bsa29I	ATCGAT	1 cut(s)	1818
BsaAI	YACGTR	2 cut(s)	701, 927
BsaBI	GATNNNNATC	2 cut(s)	222, 1139
BsaHI	GRCGYC	1 cut(s)	554
BsaI	GGTCTC	1 cut(s)	853
BsaJI	CCNNGG	3 cut(s)	1226, 2044, 2388
BsaXI	ACNNNNNCTCC	2 cut(s)	1278, 1308
Bsc4I	CCNNNNNNNGG	6 cut(s)	356, 362, 797, 1029, 1680, 2282
Bse118I	RCCGGY	2 cut(s)	77, 530
Bse1I	ACTGG	5 cut(s)	70, 602, 1408, 1646, 2201
Bse3DI	GCAATG	1 cut(s)	2076
Bse8I	GATNNNNATC	2 cut(s)	222, 1139
BseBI	CCWGG	5 cut(s)	558, 780, 1124, 1974, 2241
BseCI	ATCGAT	1 cut(s)	1818
BseDI	CCNNGG	3 cut(s)	1226, 2044, 2388
BseGI	GGATG	6 cut(s)	844, 1180, 1248, 1450, 1912, 2029
BseJI	GATNNNNATC	2 cut(s)	222, 1139
BseLI	CCNNNNNNNGG	6 cut(s)	356, 362, 797, 1029, 1680, 2282
BseMI	GCAATG	1 cut(s)	2076
BseMII	CTCAG	3 cut(s)	180, 270, 2050
BseNI	ACTGG	5 cut(s)	70, 602, 1408, 1646, 2201
BseRI	GAGGAG	2 cut(s)	839, 1800
BseXI	GCAGC	3 cut(s)	556, 1957, 2217
BseYI	CCCAGC	2 cut(s)	137, 1275
Bsh1236I	CGCG	2 cut(s)	520, 2046
Bsh1285I	CGRYCG	1 cut(s)	2305
BshFI	GGCC	3 cut(s)	998, 1122, 1595
BshNI	GGYRCC	2 cut(s)	553, 1032
BshVI	ATCGAT	1 cut(s)	1818
BsiEI	CGRYCG	1 cut(s)	2305
BsiHKAI	GWGCWC	2 cut(s)	1111, 2115
BsiHKCI	CYCGRG	1 cut(s)	1227
BsiSI	CCGG	3 cut(s)	78, 356, 531
BslFI	GGGAC	1 cut(s)	1087
BslI	CCNNNNNNNGG	6 cut(s)	356, 362, 797, 1029, 1680, 2282
BsmAI	GTCTC	2 cut(s)	27, 853
BsmFI	GGGAC	1 cut(s)	1087
BsmI	GAATGC	1 cut(s)	485
BsnI	GGCC	3 cut(s)	998, 1122, 1595
Bso31I	GGTCTC	1 cut(s)	853
BsoBI	CYCGRG	1 cut(s)	1227
Bsp119I	TTCGAA	1 cut(s)	2160
Bsp1286I	GDGCHC	3 cut(s)	1111, 1491, 2115
BspACI	CCGC	9 cut(s)	152, 958, 1872, 2002, 2005, 2044, 2046, 2221, 2385
BspANI	GGCC	3 cut(s)	998, 1122, 1595
BspCNI	CTCAG	3 cut(s)	181, 271, 2049
BspDI	ATCGAT	1 cut(s)	1818
BspFNI	CGCG	2 cut(s)	520, 2046
BspHI	TCATGA	1 cut(s)	618
BspLI	GGNNCC	5 cut(s)	555, 912, 1034, 1075, 1076
BspPI	GGATC	4 cut(s)	218, 764, 806, 2267
BspT104I	TTCGAA	1 cut(s)	2160
BspT107I	GGYRCC	2 cut(s)	553, 1032
BspTNI	GGTCTC	1 cut(s)	853
BsrDI	GCAATG	1 cut(s)	2076
BsrFI	RCCGGY	2 cut(s)	77, 530
BsrI	ACTGG	5 cut(s)	70, 602, 1408, 1646, 2201
BssAI	RCCGGY	2 cut(s)	77, 530
BssECI	CCNNGG	3 cut(s)	1226, 2044, 2388
BssNAI	GTATAC	1 cut(s)	1374
BssNI	GRCGYC	1 cut(s)	554
BssSI	CACGAG	2 cut(s)	492, 1112
Bst1107I	GTATAC	1 cut(s)	1374
Bst2BI	CACGAG	2 cut(s)	492, 1112
Bst2UI	CCWGG	5 cut(s)	558, 780, 1124, 1974, 2241
Bst4CI	ACNGT	7 cut(s)	425, 435, 1311, 1510, 1583, 2096, 2376
Bst6I	CTCTTC	4 cut(s)	48, 816, 1622, 2079
BstACI	GRCGYC	1 cut(s)	554
BstAPI	GCANNNNNTGC	1 cut(s)	2229
BstBAI	YACGTR	2 cut(s)	701, 927
BstBI	TTCGAA	1 cut(s)	2160
BstC8I	GCNNGC	5 cut(s)	79, 681, 774, 1024, 1943
BstDEI	CTNAG	7 cut(s)	189, 199, 279, 564, 864, 872, 2036
BstDSI	CCRYGG	2 cut(s)	2044, 2388
BstF5I	GGATG	6 cut(s)	844, 1180, 1248, 1450, 1912, 2029
BstFNI	CGCG	2 cut(s)	520, 2046
BstH2I	RGCGCY	1 cut(s)	557
BstHHI	GCGC	2 cut(s)	522, 556
BstMAI	GTCTC	2 cut(s)	27, 853
BstMCI	CGRYCG	1 cut(s)	2305
BstMWI	GCNNNNNNNGC	5 cut(s)	182, 560, 569, 903, 2229
BstNI	CCWGG	5 cut(s)	558, 780, 1124, 1974, 2241
BstNSI	RCATGY	2 cut(s)	893, 1189
BstPAI	GACNNNNGTC	1 cut(s)	865
BstSCI	CCNGG	6 cut(s)	355, 556, 778, 1122, 1972, 2239
BstSNI	TACGTA	1 cut(s)	927
BstUI	CGCG	2 cut(s)	520, 2046
BstV1I	GCAGC	3 cut(s)	556, 1957, 2217
BstV2I	GAAGAC	2 cut(s)	303, 1644
BstX2I	RGATCY	2 cut(s)	129, 648
BstXI	CCANNNNNNTGG	1 cut(s)	1562
BstYI	RGATCY	2 cut(s)	129, 648
BstZ17I	GTATAC	1 cut(s)	1374
Bsu15I	ATCGAT	1 cut(s)	1818
BsuI	GTATCC	2 cut(s)	712, 1226
BsuRI	GGCC	3 cut(s)	998, 1122, 1595
BsuTUI	ATCGAT	1 cut(s)	1818
BtgI	CCRYGG	2 cut(s)	2044, 2388
BtsCI	GGATG	6 cut(s)	844, 1180, 1248, 1450, 1912, 2029
BtsI	GCAGTG	1 cut(s)	529
BtsIMutI	CAGTG	5 cut(s)	63, 529, 666, 1094, 2194
Cac8I	GCNNGC	5 cut(s)	79, 681, 774, 1024, 1943
CaiI	CAGNNNCTG	1 cut(s)	1068
CciI	TCATGA	1 cut(s)	618
CfoI	GCGC	2 cut(s)	522, 556
Cfr10I	RCCGGY	2 cut(s)	77, 530
Cfr13I	GGNCC	3 cut(s)	1074, 1223, 2199
Cfr42I	CCGCGG	1 cut(s)	2047
ClaI	ATCGAT	1 cut(s)	1818
CseI	GACGC	1 cut(s)	1937
Csp6I	GTAC	3 cut(s)	203, 1033, 2317
CviQI	GTAC	3 cut(s)	203, 1033, 2317
DdeI	CTNAG	7 cut(s)	189, 199, 279, 564, 864, 872, 2036
DinI	GGCGCC	1 cut(s)	555
DraIII	CACNNNGTG	2 cut(s)	19, 359
EaeI	YGGCCR	2 cut(s)	996, 1593
Eam1104I	CTCTTC	4 cut(s)	48, 816, 1622, 2079
EarI	CTCTTC	4 cut(s)	48, 816, 1622, 2079
Ecl136II	GAGCTC	1 cut(s)	1109
Eco105I	TACGTA	1 cut(s)	927
Eco147I	AGGCCT	1 cut(s)	1122
Eco24I	GRGCYC	2 cut(s)	1111, 1491
Eco31I	GGTCTC	1 cut(s)	853
Eco32I	GATATC	3 cut(s)	616, 1141, 1292
Eco47I	GGWCC	3 cut(s)	1074, 1223, 2199
Eco53kI	GAGCTC	1 cut(s)	1109
Eco57I	CTGAAG	1 cut(s)	1518
Eco72I	CACGTG	1 cut(s)	701
Eco88I	CYCGRG	1 cut(s)	1227
EcoICRI	GAGCTC	1 cut(s)	1109
EcoO109I	RGGNCCY	1 cut(s)	1074
EcoRI	GAATTC	2 cut(s)	601, 1904
EcoRII	CCWGG	5 cut(s)	556, 778, 1122, 1972, 2239
EcoRV	GATATC	3 cut(s)	616, 1141, 1292
EcoT38I	GRGCYC	2 cut(s)	1111, 1491
EgeI	GGCGCC	1 cut(s)	555
EheI	GGCGCC	1 cut(s)	555
FaqI	GGGAC	1 cut(s)	1087
FauI	CCCGC	1 cut(s)	965
FbaI	TGATCA	1 cut(s)	2062
FblI	GTMKAC	2 cut(s)	1373, 1794
Fnu4HI	GCNGC	5 cut(s)	570, 1946, 2002, 2005, 2206
FokI	GGATG	6 cut(s)	831, 1187, 1255, 1457, 1899, 2036
FriOI	GRGCYC	2 cut(s)	1111, 1491
Fsp4HI	GCNGC	5 cut(s)	570, 1946, 2002, 2005, 2206
FspBI	CTAG	2 cut(s)	254, 1319
GlaI	GCGC	2 cut(s)	521, 555
GluI	GCNGC	5 cut(s)	570, 1946, 2002, 2005, 2206
GsaI	CCCAGC	2 cut(s)	141, 1279
GsuI	CTGGAG	3 cut(s)	579, 762, 801
HaeII	RGCGCY	1 cut(s)	557
HaeIII	GGCC	3 cut(s)	998, 1122, 1595
HapII	CCGG	3 cut(s)	78, 356, 531
HgaI	GACGC	1 cut(s)	1937
HhaI	GCGC	2 cut(s)	522, 556
Hin1I	GRCGYC	1 cut(s)	554
Hin6I	GCGC	2 cut(s)	520, 554
HinP1I	GCGC	2 cut(s)	520, 554
HindIII	AAGCTT	1 cut(s)	968
HpaII	CCGG	3 cut(s)	78, 356, 531
HphI	GGTGA	8 cut(s)	19, 343, 371, 794, 1526, 1748, 1886, 2180
Hpy166II	GTNNAC	3 cut(s)	1374, 1586, 1795
Hpy8I	GTNNAC	3 cut(s)	1374, 1586, 1795
Hpy99I	CGWCG	1 cut(s)	2345
HpyAV	CCTTC	5 cut(s)	57, 1674, 1762, 1871, 2368
HpyCH4III	ACNGT	7 cut(s)	425, 435, 1311, 1510, 1583, 2096, 2376
HpyCH4IV	ACGT	3 cut(s)	312, 700, 926
HpyCH4V	TGCA	9 cut(s)	572, 611, 683, 772, 1307, 1837, 2027, 2074, 2372
HpyF10VI	GCNNNNNNNGC	5 cut(s)	182, 560, 569, 903, 2229
HpyF3I	CTNAG	7 cut(s)	189, 199, 279, 564, 864, 872, 2036
HpySE526I	ACGT	3 cut(s)	312, 700, 926
Hsp92I	GRCGYC	1 cut(s)	554
HspAI	GCGC	2 cut(s)	520, 554
KasI	GGCGCC	1 cut(s)	553
KflI	GGGWCCC	1 cut(s)	1074
KpnI	GGTACC	1 cut(s)	1036
KroI	GCCGGC	1 cut(s)	77
KroNI	GCCGGC	1 cut(s)	79
Ksp22I	TGATCA	1 cut(s)	2062
KspI	CCGCGG	1 cut(s)	2047
LmnI	GCTCC	6 cut(s)	560, 781, 1272, 2110, 2237, 2360
Lsp1109I	GCAGC	3 cut(s)	556, 1957, 2217
LweI	GCATC	6 cut(s)	517, 581, 969, 1192, 1435, 2014
MaeI	CTAG	2 cut(s)	254, 1319
MaeII	ACGT	3 cut(s)	312, 700, 926
MaeIII	GTNAC	6 cut(s)	168, 393, 1039, 1357, 1715, 1880
MfeI	CAATTG	1 cut(s)	1746
MflI	RGATCY	2 cut(s)	129, 648
MhlI	GDGCHC	3 cut(s)	1111, 1491, 2115
MlsI	TGGCCA	2 cut(s)	998, 1595
MluNI	TGGCCA	2 cut(s)	998, 1595
Mly113I	GGCGCC	1 cut(s)	554
MlyI	GAGTC	4 cut(s)	616, 734, 1380, 2258
MmeI	TCCRAC	2 cut(s)	1375, 1549
Mox20I	TGGCCA	2 cut(s)	998, 1595
MroNI	GCCGGC	1 cut(s)	77
MscI	TGGCCA	2 cut(s)	998, 1595
MseI	TTAA	6 cut(s)	72, 735, 1470, 1760, 1886, 2187
MslI	CAYNNNNRTG	2 cut(s)	1152, 1312
Msp20I	TGGCCA	2 cut(s)	998, 1595
MspA1I	CMGCKG	1 cut(s)	2046
MspI	CCGG	3 cut(s)	78, 356, 531
MspR9I	CCNGG	6 cut(s)	357, 558, 780, 1124, 1974, 2241
MunI	CAATTG	1 cut(s)	1746
Mva1269I	GAATGC	1 cut(s)	485
MvaI	CCWGG	5 cut(s)	558, 780, 1124, 1974, 2241
MvnI	CGCG	2 cut(s)	520, 2046
MwoI	GCNNNNNNNGC	5 cut(s)	182, 560, 569, 903, 2229
NaeI	GCCGGC	1 cut(s)	79
NarI	GGCGCC	1 cut(s)	554
NciI	CCSGG	1 cut(s)	357
NgoMIV	GCCGGC	1 cut(s)	77
NlaIV	GGNNCC	5 cut(s)	555, 912, 1034, 1075, 1076
NmuCI	GTSAC	3 cut(s)	168, 1039, 1715
NspI	RCATGY	2 cut(s)	893, 1189
NspV	TTCGAA	1 cut(s)	2160
OliI	CACNNNNGTG	1 cut(s)	1312
PagI	TCATGA	1 cut(s)	618
PceI	AGGCCT	1 cut(s)	1122
PciI	ACATGT	2 cut(s)	889, 1185
PctI	GAATGC	1 cut(s)	485
PdiI	GCCGGC	1 cut(s)	79
PfeI	GAWTC	7 cut(s)	10, 161, 709, 1250, 1480, 1969, 2289
PflMI	CCANNNNNTGG	1 cut(s)	797
PkrI	GCNGC	5 cut(s)	571, 1947, 2003, 2006, 2207
Ple19I	CGATCG	1 cut(s)	2305
PleI	GAGTC	4 cut(s)	616, 734, 1380, 2258
PluTI	GGCGCC	1 cut(s)	557
PmaCI	CACGTG	1 cut(s)	701
PmlI	CACGTG	1 cut(s)	701
PpsI	GAGTC	4 cut(s)	616, 734, 1380, 2258
Ppu21I	YACGTR	2 cut(s)	701, 927
PpuMI	RGGWCCY	1 cut(s)	1074
PscI	ACATGT	2 cut(s)	889, 1185
PshAI	GACNNNNGTC	1 cut(s)	865
PsiI	TTATAA	1 cut(s)	1856
Psp124BI	GAGCTC	1 cut(s)	1111
Psp1406I	AACGTT	1 cut(s)	312
Psp5II	RGGWCCY	1 cut(s)	1074
Psp6I	CCWGG	5 cut(s)	556, 778, 1122, 1972, 2239
PspCI	CACGTG	1 cut(s)	701
PspFI	CCCAGC	2 cut(s)	137, 1275
PspGI	CCWGG	5 cut(s)	556, 778, 1122, 1972, 2239
PspN4I	GGNNCC	5 cut(s)	555, 912, 1034, 1075, 1076
PspPI	GGNCC	3 cut(s)	1074, 1223, 2199
PspPPI	RGGWCCY	1 cut(s)	1074
PstNI	CAGNNNCTG	1 cut(s)	1068
PsuI	RGATCY	2 cut(s)	129, 648
PvuI	CGATCG	1 cut(s)	2305
RsaI	GTAC	3 cut(s)	204, 1034, 2318
RsaNI	GTAC	3 cut(s)	203, 1033, 2317
RseI	CAYNNNNRTG	2 cut(s)	1152, 1312
SacI	GAGCTC	1 cut(s)	1111
SacII	CCGCGG	1 cut(s)	2047
SaqAI	TTAA	6 cut(s)	72, 735, 1470, 1760, 1886, 2187
SatI	GCNGC	5 cut(s)	570, 1946, 2002, 2005, 2206
Sau96I	GGNCC	3 cut(s)	1074, 1223, 2199
ScaI	AGTACT	1 cut(s)	2318
SchI	GAGTC	4 cut(s)	616, 734, 1380, 2258
ScrFI	CCNGG	6 cut(s)	357, 558, 780, 1124, 1974, 2241
SduI	GDGCHC	3 cut(s)	1111, 1491, 2115
SfaNI	GCATC	6 cut(s)	517, 581, 969, 1192, 1435, 2014
SfoI	GGCGCC	1 cut(s)	555
Sfr303I	CCGCGG	1 cut(s)	2047
SfuI	TTCGAA	1 cut(s)	2160
SgrBI	CCGCGG	1 cut(s)	2047
SinI	GGWCC	3 cut(s)	1074, 1223, 2199
SmiMI	CAYNNNNRTG	2 cut(s)	1152, 1312
SmlI	CTYRAG	2 cut(s)	479, 1622
SmoI	CTYRAG	2 cut(s)	479, 1622
SnaBI	TACGTA	1 cut(s)	927
SseBI	AGGCCT	1 cut(s)	1122
SsiI	CCGC	9 cut(s)	152, 958, 1872, 2002, 2005, 2044, 2046, 2221, 2385
SspDI	GGCGCC	1 cut(s)	553
SspMI	CTAG	2 cut(s)	254, 1319
SstI	GAGCTC	1 cut(s)	1111
StuI	AGGCCT	1 cut(s)	1122
StyD4I	CCNGG	6 cut(s)	355, 556, 778, 1122, 1972, 2239
TaaI	ACNGT	7 cut(s)	425, 435, 1311, 1510, 1583, 2096, 2376
TaiI	ACGT	3 cut(s)	315, 703, 929
TaqI	TCGA	4 cut(s)	52, 1818, 2160, 2301
TatI	WGTACW	1 cut(s)	2316
TauI	GCSGC	2 cut(s)	2004, 2007
TfiI	GAWTC	7 cut(s)	10, 161, 709, 1250, 1480, 1969, 2289
Tru1I	TTAA	6 cut(s)	72, 735, 1470, 1760, 1886, 2187
Tru9I	TTAA	6 cut(s)	72, 735, 1470, 1760, 1886, 2187
TscAI	CASTG	5 cut(s)	70, 529, 673, 1101, 2201
TseFI	GTSAC	3 cut(s)	168, 1039, 1715
TseI	GCWGC	3 cut(s)	569, 1945, 2205
Tsp45I	GTSAC	3 cut(s)	168, 1039, 1715
TspGWI	ACGGA	2 cut(s)	30, 923
TspRI	CASTG	5 cut(s)	70, 529, 673, 1101, 2201
Van91I	CCANNNNNTGG	1 cut(s)	797
VpaK11BI	GGWCC	3 cut(s)	1074, 1223, 2199
XapI	RAATTY	6 cut(s)	372, 601, 632, 1888, 1904, 2057
XbaI	TCTAGA	1 cut(s)	253
XceI	RCATGY	2 cut(s)	893, 1189
XmiI	GTMKAC	2 cut(s)	1373, 1794
XspI	CTAG	2 cut(s)	254, 1319
ZrmI	AGTACT	1 cut(s)	2318

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.