Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005666

Physical Location & Seq

Genomic Coordinates Reverse (-)

1658062 .. 1667026

Gene Structure 8965 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1017700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2538 bp

ATGGTCTCGACTCTCTCGGGGATTCGCTTCCCTCTTCTGCCTTCTGCCTACACCTCGCATGCCAGTTTTATTGGCGATCGGAGGACCTCCGGCGGCCTCTCTCTCTTCCTCTCCAACACCTCCTTCTCTCGGAAGATCTTTGCTGGAAAGTCTTCGTATGATTCTGATTTGCCGTCTTTAGCAGTTGCTGCTTCGAAGAAAATTCTTGTTCCGGACAGCCAGAGTGATGGCTCTTCGTCTTTGACGGAGCAATTGGGAGCTACGAGTACAGTTTCAGAGGATCCTCAGGTGATTGAGGATGTAGATAATGTAGTAATGGAAGATGAAGAGAAGCTTGAGGATGTCCCTTCATTAGTTGTGGACAATGTTGATGATGCTGAAGCCAAGGTTGAGGACGTACCTCGTCCCTTAGAAGTTACAGCAAGTACTGCTACAGGCAAAGCTAAGGGAAAGACAATTCCACCGCCTGGCAAGGGGCAAAAGATATATGAGATAGATCCACTTTTGGTGGGTCACCGTGATCATCTTGATTACCGTTATGGACAATACAAAAGGCTGCGTGAGCAGATTGACAAGTGCGAAGGCGGTTTAGAGGTGTTTTCTCGTGGCTATGAGAAGTTTGGTTTCACACGCAGTGCGGAAGGTATCACTTATAGGGAGTGGGCTCCTGGAGCAAAGTCAGCATCACTGATTGGAGATTTCAACAATTGGAACACAAATGCAGATGTGATGACTCGGAATGAATTTGGAGTCTGGGAGATCTTTTTGCCAAACAATGCAGATGGCTCACCTTCGATTCCACATGGTTCTCGAGTGAAGATCCGTATGGATACTCCTTCAGGCATCAAAGACTCGATTCCTGCTTGGATCAAGTTTTCTGTGCAGGCTCCAGGTGAAATCCCATACAATGGCATATACTATGATCCACCAGAAGAGGAAAAGTATGTATTTCAACATTCTCAGCCGAGGAGACCAAAATCACTTCGCATTTATGAGGCACATGTTGGAATGAGTAGCCCGGAGGGCAAAATTAACTCATATGCTGAATTCAGAGATGATGTGCTTCCACGCATTAAGAAGCTTGGTTACAATGCTGTTCAGCTTATGGCTATTCAAGAGCACTCCTACTATGCTAGCTTTGGGTACCATGTCACCAATTTTTTTGCACCTAGCAGCCGCTGTGGAACCCCTGACGATCTGAAGTCTCTGATAGATAAAGCTCACGAGCTTGGTCTGCTTGTACTTATGGATATTGTTCACAGCCATGCATCGAATAACACGTTGGACGGGTTGAACATGTTTGATGGAACCGATAGTCATTATTTCCACTCTGGGTCTCGAGGATATCATTGGATGTGGGATTCCCGCTTGTTTAATTATGGAAGCTGGGAGGTTTTGAGGTATCTTCTTTCCAATGCAAGGTGGTGGCTGGAAGAATACAAGTTCGATGGGTTCAGATTTGATGGTGTCACTTCAATGATGTACACTCACCATGGATTGGAGGTAGCATTTACTGGAAATTACAGTGAGTACTTTGGATTAGCAACAGATGTAGATGCTGTGACTTACCTGATGCTGGTTAACGATCTGATTCATGGGCTTTACCCTGAAGCTGTTACCGTTGGTGAAGATGTTAGTGGAATGCCAACTTTCTGTATTGCTGTTAACGATGGTGGTGTTGGATTTGATTATCGCCTGCAAATGGCTATTGCTGATAAATGGATTGAGCTCCTTAAGAAAATGGATGAAGAGTGGAAAATGGGTGATATTGTTTTCACACTCACCAACAGAAGGTGGCGGGAGAACTGTGTTGCTTATGCCGAAAGTCATGACCAAGCTCTTGTTGGTGACAAAACAATTGCATTTTGGCTGATGGACAAGGATATGTATGATTTCATGGCTTTGGACAGACCATCAACTCCCCGTATAGATCGTGGAATAGCATTACATAAAATGATTAGGCTTATTACTATGGGATTAGGCGGAGAAGGTTATCTGAATTTCATGGGAAATGAATTTGGCCATCCTGAGTGGATTGATTTTCCAAGAGGTGTTCAACATCTTCCAAATGGTAAAATAGTTCCTGGGAATAATAACAGTTTTGACAAATGCCGACGTAGATTTGATCTTGGGGATGCAGAGTATCTAAGATATCACGGGATGCAAGAATTTGATCGAGCAATGCAACATCTCGAAGAAACCTATGGTTTCATGACTTCTGAGCACCAATATATATCACGGAAGGATGAAAGAGACAGAATTATCGTCTTTGAAAGGGGAGACCTGGTTTTTGTCTTCAATTTTCATTGGAGTAAAAGCTATTCAGACTACCGAATTGGCTGCTTAAAGCCAGGAAAGTACAAGATTGTCTTGGATTCAGATGAAAAGCTATTTGGGGGCTTCGATAGGCTTGATCACAGTGCTGAGTACTTCACGACTGATGGGTGGTTCGATGACCGGCCTCATTCCTTCCTCCTCTATGCACCTTGTAGAACAGCTGTGGTCTACGCTCTCGTAGAGGGACCTAAAAAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010021 GO:0010033 GO:0016740 GO:0016757 GO:0016758 GO:0019725 GO:0033500 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0042593 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071326 GO:0071329 GO:0071331 GO:0071332 GO:0071333 GO:0071478 GO:0071482 GO:0071704 GO:0104004 GO:1901135 GO:1901137 GO:1901576 GO:1901700 GO:1901701 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

846

Amino Acids

95.94

Weight (kDa)

5.39

Isoelectric Point (pI)

32.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	206 - 289	3.7e-20	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	355 - 426	3.4e-09	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	744 - 838	4.3e-23	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36390 AT5G03650
fragaria_vesca	FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120
malus_domestica	MD12G1020600.v1.1 MD14G1017700.v1.1
prunus_persica	Prupe.7G115400_v2.0.a1 Prupe.7G115400_v2.0.a1
pyrus_communis	pycom12g01930 pycom14g01610
rosa_chinensis	RchiOBHm_Chr3g0472421
rosa_laevigata	RLG00000024081
rosa_multiflora	Rmu_co8370739.1_g000001 Rmu_sc0001096.1_g000015
rosa_roxburghii	Rroxscaffold_6G00408930
rosa_rugosa	Rorug03G0124700 Rorug03G0124700 Rorug03G0124800 Rorug03G0124900
rosa_samantha	Rh3AG177100 Rh3BG203100 Rh3CG193400 Rh3CG200600 Rh3DG172000
rosa_wichuraiana	Rw3G016440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1143
AccB1I	GGYRCC	1 cut(s)	1143
AccB7I	CCANNNNNTGG	3 cut(s)	467, 908, 1498
AccI	GTMKAC	1 cut(s)	2505
AccIII	TCCGGA	1 cut(s)	211
AciI	CCGC	8 cut(s)	93, 464, 585, 638, 1177, 1366, 1798, 1983
AclWI	GGATC	6 cut(s)	275, 288, 491, 814, 875, 917
AcoI	YGGCCR	1 cut(s)	2020
AcsI	RAATTY	6 cut(s)	201, 743, 1046, 1999, 2015, 2168
AcuI	CTGAAG	4 cut(s)	399, 822, 1220, 1629
AdeI	CACNNNGTG	1 cut(s)	635
AfaI	GTAC	9 cut(s)	268, 399, 427, 1145, 1242, 1484, 1532, 2360, 2429
AflII	CTTAAG	1 cut(s)	1733
AflIII	ACRYGT	3 cut(s)	1000, 1278, 1296
AgsI	TTSAA	8 cut(s)	703, 953, 1115, 1294, 1476, 2057, 2273, 2299
AjnI	CCWGG	6 cut(s)	466, 667, 889, 2083, 2283, 2350
AjuI	GAANNNNNNNTTGG	6 cut(s)	107, 139, 1265, 1297, 2376, 2408
Alw21I	GWGCWC	3 cut(s)	1122, 1731, 2226
Alw26I	GTCTC	6 cut(s)	10, 964, 1209, 1341, 2247, 2274
AlwI	GGATC	6 cut(s)	275, 288, 491, 814, 875, 917
AlwNI	CAGNNNCTG	2 cut(s)	188, 1179
Ama87I	CYCGRG	3 cut(s)	16, 810, 1338
Aor13HI	TCCGGA	1 cut(s)	211
AoxI	GGCC	3 cut(s)	94, 2020, 2459
ApeKI	GCWGC	4 cut(s)	188, 556, 1173, 2340
ApoI	RAATTY	6 cut(s)	201, 743, 1046, 1999, 2015, 2168
ArsI	GACNNNNNNTTYG	2 cut(s)	2273, 2305
Asp700I	GAANNNNTTC	1 cut(s)	151
Asp718I	GGTACC	1 cut(s)	1143
AspS9I	GGNCC	2 cut(s)	84, 2522
AsuC2I	CCSGG	1 cut(s)	1019
AsuII	TTCGAA	1 cut(s)	194
AsuNHI	GCTAGC	1 cut(s)	1133
AvaI	CYCGRG	3 cut(s)	16, 810, 1338
AvaII	GGWCC	2 cut(s)	84, 2522
AxyI	CCTNAGG	1 cut(s)	285
BalI	TGGCCA	1 cut(s)	2022
BamHI	GGATCC	1 cut(s)	280
BanI	GGYRCC	1 cut(s)	1143
BanII	GRGCYC	2 cut(s)	667, 1731
BarI	GAAGNNNNNNTAC	2 cut(s)	1070, 1102
BauI	CACGAG	2 cut(s)	603, 1223
BbsI	GAAGAC	2 cut(s)	144, 2287
Bbv12I	GWGCWC	3 cut(s)	1122, 1731, 2226
BbvI	GCAGC	4 cut(s)	175, 543, 1185, 2327
BceAI	ACGGC	1 cut(s)	157
BcgI	CGANNNNNNTGC	2 cut(s)	2322, 2356
BciT130I	CCWGG	6 cut(s)	468, 669, 891, 2085, 2285, 2352
BciVI	GTATCC	1 cut(s)	823
BclI	TGATCA	2 cut(s)	520, 2413
BcnI	CCSGG	1 cut(s)	1019
BcoDI	GTCTC	6 cut(s)	10, 964, 1209, 1341, 2247, 2274
BfaI	CTAG	2 cut(s)	1134, 1170
BfmI	CTRYAG	1 cut(s)	432
BfrI	CTTAAG	1 cut(s)	1733
BfuI	GTATCC	1 cut(s)	823
BglI	GCCNNNNNGGC	1 cut(s)	1023
BglII	AGATCT	2 cut(s)	135, 759
BisI	GCNGC	6 cut(s)	94, 189, 557, 1174, 1177, 2341
BlsI	GCNGC	6 cut(s)	95, 190, 558, 1175, 1178, 2342
BmcAI	AGTACT	3 cut(s)	427, 1532, 2429
Bme1390I	CCNGG	7 cut(s)	468, 669, 891, 1019, 2085, 2285, 2352
Bme18I	GGWCC	2 cut(s)	84, 2522
BmeT110I	CYCGRG	3 cut(s)	16, 810, 1338
BmgT120I	GGNCC	2 cut(s)	84, 2522
BmiI	GGNNCC	7 cut(s)	282, 666, 888, 1145, 1186, 1309, 2523
BmrFI	CCNGG	7 cut(s)	468, 669, 891, 1019, 2085, 2285, 2352
BmsI	GCATC	8 cut(s)	364, 692, 852, 1277, 1546, 1563, 2125, 2151
BmtI	GCTAGC	1 cut(s)	1137
BpiI	GAAGAC	2 cut(s)	144, 2287
BpmI	CTGGAG	2 cut(s)	690, 873
Bpu10I	CCTNAGC	1 cut(s)	444
Bpu14I	TTCGAA	1 cut(s)	194
BpuEI	CTTGAG	1 cut(s)	356
BpuMI	CCSGG	1 cut(s)	1019
BsaI	GGTCTC	4 cut(s)	10, 964, 1341, 2274
BsaJI	CCNNGG	4 cut(s)	384, 965, 1492, 2084
BsaWI	WCCGGW	1 cut(s)	211
BsaXI	ACNNNNNCTCC	2 cut(s)	2271, 2301
Bse118I	RCCGGY	1 cut(s)	2457
Bse1I	ACTGG	2 cut(s)	63, 1519
Bse21I	CCTNAGG	1 cut(s)	285
Bse3DI	GCAATG	1 cut(s)	2187
BseAI	TCCGGA	1 cut(s)	211
BseBI	CCWGG	6 cut(s)	468, 669, 891, 2085, 2285, 2352
BseDI	CCNNGG	4 cut(s)	384, 965, 1492, 2084
BseGI	GGATG	8 cut(s)	304, 346, 1359, 1750, 2023, 2140, 2166, 2251
BseMI	GCAATG	1 cut(s)	2187
BseMII	CTCAG	5 cut(s)	299, 974, 2019, 2211, 2415
BseNI	ACTGG	2 cut(s)	63, 1519
BseRI	GAGGAG	2 cut(s)	982, 2465
BseXI	GCAGC	4 cut(s)	175, 543, 1185, 2327
BseYI	CCCAGC	1 cut(s)	1386
BsgI	GTGCAG	1 cut(s)	902
Bsh1285I	CGRYCG	1 cut(s)	79
BshFI	GGCC	3 cut(s)	96, 2022, 2461
BshNI	GGYRCC	1 cut(s)	1143
BsiEI	CGRYCG	1 cut(s)	79
BsiHKAI	GWGCWC	3 cut(s)	1122, 1731, 2226
BsiHKCI	CYCGRG	3 cut(s)	16, 810, 1338
BsiSI	CCGG	4 cut(s)	90, 212, 1019, 2458
BslFI	GGGAC	2 cut(s)	329, 390
BsmAI	GTCTC	6 cut(s)	10, 964, 1209, 1341, 2247, 2274
BsmFI	GGGAC	2 cut(s)	329, 390
BsmI	GAATGC	1 cut(s)	1647
BsnI	GGCC	3 cut(s)	96, 2022, 2461
Bso31I	GGTCTC	4 cut(s)	10, 964, 1341, 2274
BsoBI	CYCGRG	3 cut(s)	16, 810, 1338
Bsp119I	TTCGAA	1 cut(s)	194
Bsp1286I	GDGCHC	4 cut(s)	667, 1122, 1731, 2226
Bsp13I	TCCGGA	1 cut(s)	211
Bsp1407I	TGTACA	1 cut(s)	1482
Bsp19I	CCATGG	1 cut(s)	1492
BspACI	CCGC	8 cut(s)	93, 464, 585, 638, 1177, 1366, 1798, 1983
BspANI	GGCC	3 cut(s)	96, 2022, 2461
BspCNI	CTCAG	5 cut(s)	298, 973, 2020, 2212, 2416
BspEI	TCCGGA	1 cut(s)	211
BspHI	TCATGA	2 cut(s)	1828, 2211
BspLI	GGNNCC	7 cut(s)	282, 666, 888, 1145, 1186, 1309, 2523
BspOI	GCTAGC	1 cut(s)	1137
BspPI	GGATC	6 cut(s)	275, 288, 491, 814, 875, 917
BspQI	GCTCTTC	1 cut(s)	238
BspT104I	TTCGAA	1 cut(s)	194
BspT107I	GGYRCC	1 cut(s)	1143
BspTI	CTTAAG	1 cut(s)	1733
BspTNI	GGTCTC	4 cut(s)	10, 964, 1341, 2274
BsrDI	GCAATG	1 cut(s)	2187
BsrFI	RCCGGY	1 cut(s)	2457
BsrGI	TGTACA	1 cut(s)	1482
BsrI	ACTGG	2 cut(s)	63, 1519
BssAI	RCCGGY	1 cut(s)	2457
BssECI	CCNNGG	4 cut(s)	384, 965, 1492, 2084
BssSI	CACGAG	2 cut(s)	603, 1223
BssT1I	CCWWGG	2 cut(s)	384, 1492
Bst2BI	CACGAG	2 cut(s)	603, 1223
Bst2UI	CCWGG	6 cut(s)	468, 669, 891, 2085, 2285, 2352
Bst4CI	ACNGT	8 cut(s)	271, 518, 536, 1526, 1621, 1808, 2099, 2420
Bst6I	CTCTTC	6 cut(s)	39, 110, 238, 321, 927, 1743
BstAFI	CTTAAG	1 cut(s)	1733
BstAPI	GCANNNNNTGC	2 cut(s)	188, 428
BstAUI	TGTACA	1 cut(s)	1482
BstBI	TTCGAA	1 cut(s)	194
BstC8I	GCNNGC	4 cut(s)	60, 885, 1135, 1697
BstDEI	CTNAG	8 cut(s)	285, 409, 444, 960, 2028, 2147, 2220, 2424
BstDSI	CCRYGG	1 cut(s)	1492
BstEII	GGTNACC	1 cut(s)	512
BstENI	CCTNNNNNAGG	1 cut(s)	2529
BstF5I	GGATG	8 cut(s)	304, 346, 1359, 1750, 2023, 2140, 2166, 2251
BstMAI	GTCTC	6 cut(s)	10, 964, 1209, 1341, 2247, 2274
BstMCI	CGRYCG	1 cut(s)	79
BstMWI	GCNNNNNNNGC	7 cut(s)	188, 428, 562, 671, 680, 1023, 1234
BstNI	CCWGG	6 cut(s)	468, 669, 891, 2085, 2285, 2352
BstNSI	RCATGY	3 cut(s)	62, 1004, 1300
BstPI	GGTNACC	1 cut(s)	512
BstSCI	CCNGG	7 cut(s)	466, 667, 889, 1017, 2083, 2283, 2350
BstSFI	CTRYAG	1 cut(s)	432
BstV1I	GCAGC	4 cut(s)	175, 543, 1185, 2327
BstV2I	GAAGAC	2 cut(s)	144, 2287
BstX2I	RGATCY	5 cut(s)	135, 280, 496, 759, 819
BstXI	CCANNNNNNTGG	1 cut(s)	227
BstYI	RGATCY	5 cut(s)	135, 280, 496, 759, 819
Bsu36I	CCTNAGG	1 cut(s)	285
BsuI	GTATCC	1 cut(s)	823
BsuRI	GGCC	3 cut(s)	96, 2022, 2461
BtgI	CCRYGG	1 cut(s)	1492
BtsCI	GGATG	8 cut(s)	304, 346, 1359, 1750, 2023, 2140, 2166, 2251
BtsI	GCAGTG	1 cut(s)	640
BtsIMutI	CAGTG	4 cut(s)	640, 686, 1531, 2425
Cac8I	GCNNGC	4 cut(s)	60, 885, 1135, 1697
CaiI	CAGNNNCTG	2 cut(s)	188, 1179
CciI	TCATGA	2 cut(s)	1828, 2211
Cfr10I	RCCGGY	1 cut(s)	2457
Cfr13I	GGNCC	2 cut(s)	84, 2522
CsiI	ACCWGGT	1 cut(s)	2283
Csp6I	GTAC	9 cut(s)	267, 398, 426, 1144, 1241, 1483, 1531, 2359, 2428
CviQI	GTAC	9 cut(s)	267, 398, 426, 1144, 1241, 1483, 1531, 2359, 2428
DdeI	CTNAG	8 cut(s)	285, 409, 444, 960, 2028, 2147, 2220, 2424
DraIII	CACNNNGTG	1 cut(s)	635
EaeI	YGGCCR	1 cut(s)	2020
Eam1104I	CTCTTC	6 cut(s)	39, 110, 238, 321, 927, 1743
EarI	CTCTTC	6 cut(s)	39, 110, 238, 321, 927, 1743
EciI	GGCGGA	1 cut(s)	1998
Ecl136II	GAGCTC	1 cut(s)	1729
Eco130I	CCWWGG	2 cut(s)	384, 1492
Eco24I	GRGCYC	2 cut(s)	667, 1731
Eco31I	GGTCTC	4 cut(s)	10, 964, 1341, 2274
Eco32I	GATATC	2 cut(s)	1346, 2153
Eco47I	GGWCC	2 cut(s)	84, 2522
Eco53kI	GAGCTC	1 cut(s)	1729
Eco57I	CTGAAG	4 cut(s)	399, 822, 1220, 1629
Eco81I	CCTNAGG	1 cut(s)	285
Eco88I	CYCGRG	3 cut(s)	16, 810, 1338
Eco91I	GGTNACC	1 cut(s)	512
EcoICRI	GAGCTC	1 cut(s)	1729
EcoNI	CCTNNNNNAGG	1 cut(s)	2529
EcoO109I	RGGNCCY	2 cut(s)	84, 2522
EcoO65I	GGTNACC	1 cut(s)	512
EcoRI	GAATTC	1 cut(s)	1046
EcoRII	CCWGG	6 cut(s)	466, 667, 889, 2083, 2283, 2350
EcoRV	GATATC	2 cut(s)	1346, 2153
EcoT14I	CCWWGG	2 cut(s)	384, 1492
EcoT22I	ATGCAT	1 cut(s)	1270
EcoT38I	GRGCYC	2 cut(s)	667, 1731
ErhI	CCWWGG	2 cut(s)	384, 1492
FalI	AAGNNNNNCTT	4 cut(s)	318, 350, 2354, 2386
FaqI	GGGAC	2 cut(s)	329, 390
FauI	CCCGC	2 cut(s)	1373, 1791
FauNDI	CATATG	1 cut(s)	1039
FbaI	TGATCA	2 cut(s)	520, 2413
FblI	GTMKAC	1 cut(s)	2505
Fnu4HI	GCNGC	6 cut(s)	94, 189, 557, 1174, 1177, 2341
FokI	GGATG	8 cut(s)	311, 353, 1366, 1757, 2010, 2147, 2173, 2258
FriOI	GRGCYC	2 cut(s)	667, 1731
Fsp4HI	GCNGC	6 cut(s)	94, 189, 557, 1174, 1177, 2341
FspBI	CTAG	2 cut(s)	1134, 1170
GluI	GCNGC	6 cut(s)	94, 189, 557, 1174, 1177, 2341
GsaI	CCCAGC	1 cut(s)	1390
GsuI	CTGGAG	2 cut(s)	690, 873
HaeIII	GGCC	3 cut(s)	96, 2022, 2461
HapII	CCGG	4 cut(s)	90, 212, 1019, 2458
HincII	GTYRAC	2 cut(s)	1582, 1666
HindII	GTYRAC	2 cut(s)	1582, 1666
HindIII	AAGCTT	2 cut(s)	332, 1079
HpaI	GTTAAC	2 cut(s)	1582, 1666
HpaII	CCGG	4 cut(s)	90, 212, 1019, 2458
Hpy166II	GTNNAC	6 cut(s)	361, 1258, 1485, 1582, 1666, 2506
Hpy8I	GTNNAC	6 cut(s)	361, 1258, 1485, 1582, 1666, 2506
Hpy99I	CGWCG	1 cut(s)	2118
HpyCH4III	ACNGT	8 cut(s)	271, 518, 536, 1526, 1621, 1808, 2099, 2420
HpyCH4IV	ACGT	3 cut(s)	396, 1280, 2116
HpyF10VI	GCNNNNNNNGC	7 cut(s)	188, 428, 562, 671, 680, 1023, 1234
HpyF3I	CTNAG	8 cut(s)	285, 409, 444, 960, 2028, 2147, 2220, 2424
HpySE526I	ACGT	3 cut(s)	396, 1280, 2116
Kpn2I	TCCGGA	1 cut(s)	211
KpnI	GGTACC	1 cut(s)	1147
Ksp22I	TGATCA	2 cut(s)	520, 2413
KspAI	GTTAAC	2 cut(s)	1582, 1666
LguI	GCTCTTC	1 cut(s)	238
LmnI	GCTCC	6 cut(s)	247, 257, 670, 671, 892, 1734
Lsp1109I	GCAGC	4 cut(s)	175, 543, 1185, 2327
LweI	GCATC	8 cut(s)	364, 692, 852, 1277, 1546, 1563, 2125, 2151
MabI	ACCWGGT	1 cut(s)	2283
MaeI	CTAG	2 cut(s)	1134, 1170
MaeII	ACGT	3 cut(s)	396, 1280, 2116
MaeIII	GTNAC	8 cut(s)	415, 512, 1085, 1150, 1468, 1561, 1615, 1847
MfeI	CAATTG	3 cut(s)	251, 706, 1857
MflI	RGATCY	5 cut(s)	135, 280, 496, 759, 819
MhlI	GDGCHC	4 cut(s)	667, 1122, 1731, 2226
MlsI	TGGCCA	1 cut(s)	2022
MluNI	TGGCCA	1 cut(s)	2022
MlyI	GAGTC	4 cut(s)	4, 727, 759, 845
MmeI	TCCRAC	4 cut(s)	138, 985, 1263, 1660
Mox20I	TGGCCA	1 cut(s)	2022
Mph1103I	ATGCAT	1 cut(s)	1270
MroI	TCCGGA	1 cut(s)	211
MroXI	GAANNNNTTC	1 cut(s)	151
MscI	TGGCCA	1 cut(s)	2022
MseI	TTAA	7 cut(s)	1032, 1074, 1374, 1581, 1665, 1734, 2345
MslI	CAYNNNNRTG	2 cut(s)	1263, 1475
Msp20I	TGGCCA	1 cut(s)	2022
MspA1I	CMGCKG	2 cut(s)	1179, 2498
MspCI	CTTAAG	1 cut(s)	1733
MspI	CCGG	4 cut(s)	90, 212, 1019, 2458
MspR9I	CCNGG	7 cut(s)	468, 669, 891, 1019, 2085, 2285, 2352
MunI	CAATTG	3 cut(s)	251, 706, 1857
Mva1269I	GAATGC	1 cut(s)	1647
MvaI	CCWGG	6 cut(s)	468, 669, 891, 2085, 2285, 2352
MwoI	GCNNNNNNNGC	7 cut(s)	188, 428, 562, 671, 680, 1023, 1234
NciI	CCSGG	1 cut(s)	1019
NcoI	CCATGG	1 cut(s)	1492
NdeI	CATATG	1 cut(s)	1039
NheI	GCTAGC	1 cut(s)	1133
NlaIV	GGNNCC	7 cut(s)	282, 666, 888, 1145, 1186, 1309, 2523
NmeAIII	GCCGAG	1 cut(s)	990
NmuCI	GTSAC	5 cut(s)	512, 1150, 1468, 1561, 1847
NsiI	ATGCAT	1 cut(s)	1270
NspI	RCATGY	3 cut(s)	62, 1004, 1300
NspV	TTCGAA	1 cut(s)	194
PaeI	GCATGC	1 cut(s)	62
PaeR7I	CTCGAG	2 cut(s)	810, 1338
PagI	TCATGA	2 cut(s)	1828, 2211
PciI	ACATGT	2 cut(s)	1000, 1296
PciSI	GCTCTTC	1 cut(s)	238
PcsI	WCGNNNNNNNCGW	1 cut(s)	1277
PctI	GAATGC	1 cut(s)	1647
PdmI	GAANNNNTTC	1 cut(s)	151
PfeI	GAWTC	7 cut(s)	22, 161, 796, 856, 1361, 1591, 2375
PflMI	CCANNNNNTGG	3 cut(s)	467, 908, 1498
PfoI	TCCNGGA	1 cut(s)	667
PkrI	GCNGC	6 cut(s)	95, 190, 558, 1175, 1178, 2342
Ple19I	CGATCG	1 cut(s)	79
PleI	GAGTC	4 cut(s)	4, 727, 758, 845
PpsI	GAGTC	4 cut(s)	4, 727, 758, 845
PpuMI	RGGWCCY	2 cut(s)	84, 2522
PscI	ACATGT	2 cut(s)	1000, 1296
Psp124BI	GAGCTC	1 cut(s)	1731
Psp5II	RGGWCCY	2 cut(s)	84, 2522
Psp6I	CCWGG	6 cut(s)	466, 667, 889, 2083, 2283, 2350
PspEI	GGTNACC	1 cut(s)	512
PspFI	CCCAGC	1 cut(s)	1386
PspGI	CCWGG	6 cut(s)	466, 667, 889, 2083, 2283, 2350
PspN4I	GGNNCC	7 cut(s)	282, 666, 888, 1145, 1186, 1309, 2523
PspPI	GGNCC	2 cut(s)	84, 2522
PspPPI	RGGWCCY	2 cut(s)	84, 2522
PstNI	CAGNNNCTG	2 cut(s)	188, 1179
PsuI	RGATCY	5 cut(s)	135, 280, 496, 759, 819
PvuI	CGATCG	1 cut(s)	79
PvuII	CAGCTG	1 cut(s)	2498
RsaI	GTAC	9 cut(s)	268, 399, 427, 1145, 1242, 1484, 1532, 2360, 2429
RsaNI	GTAC	9 cut(s)	267, 398, 426, 1144, 1241, 1483, 1531, 2359, 2428
RseI	CAYNNNNRTG	2 cut(s)	1263, 1475
SacI	GAGCTC	1 cut(s)	1731
SapI	GCTCTTC	1 cut(s)	238
SaqAI	TTAA	7 cut(s)	1032, 1074, 1374, 1581, 1665, 1734, 2345
SatI	GCNGC	6 cut(s)	94, 189, 557, 1174, 1177, 2341
Sau96I	GGNCC	2 cut(s)	84, 2522
ScaI	AGTACT	3 cut(s)	427, 1532, 2429
SchI	GAGTC	4 cut(s)	4, 727, 759, 845
ScrFI	CCNGG	7 cut(s)	468, 669, 891, 1019, 2085, 2285, 2352
SduI	GDGCHC	4 cut(s)	667, 1122, 1731, 2226
SexAI	ACCWGGT	1 cut(s)	2283
SfaNI	GCATC	8 cut(s)	364, 692, 852, 1277, 1546, 1563, 2125, 2151
SfcI	CTRYAG	1 cut(s)	432
Sfr274I	CTCGAG	2 cut(s)	810, 1338
SfuI	TTCGAA	1 cut(s)	194
SinI	GGWCC	2 cut(s)	84, 2522
SlaI	CTCGAG	2 cut(s)	810, 1338
SmiMI	CAYNNNNRTG	2 cut(s)	1263, 1475
SmlI	CTYRAG	4 cut(s)	335, 810, 1338, 1733
SmoI	CTYRAG	4 cut(s)	335, 810, 1338, 1733
SphI	GCATGC	1 cut(s)	62
SsiI	CCGC	8 cut(s)	93, 464, 585, 638, 1177, 1366, 1798, 1983
SspMI	CTAG	2 cut(s)	1134, 1170
SstI	GAGCTC	1 cut(s)	1731
StyD4I	CCNGG	7 cut(s)	466, 667, 889, 1017, 2083, 2283, 2350
StyI	CCWWGG	2 cut(s)	384, 1492
TaaI	ACNGT	8 cut(s)	271, 518, 536, 1526, 1621, 1808, 2099, 2420
TaiI	ACGT	3 cut(s)	399, 1283, 2119
TatI	WGTACW	7 cut(s)	266, 425, 1240, 1482, 1530, 2358, 2427
TauI	GCSGC	2 cut(s)	96, 1179
TfiI	GAWTC	7 cut(s)	22, 161, 796, 856, 1361, 1591, 2375
Tru1I	TTAA	7 cut(s)	1032, 1074, 1374, 1581, 1665, 1734, 2345
Tru9I	TTAA	7 cut(s)	1032, 1074, 1374, 1581, 1665, 1734, 2345
TscAI	CASTG	4 cut(s)	640, 693, 1531, 2425
TseFI	GTSAC	5 cut(s)	512, 1150, 1468, 1561, 1847
TseI	GCWGC	4 cut(s)	188, 556, 1173, 2340
Tsp45I	GTSAC	5 cut(s)	512, 1150, 1468, 1561, 1847
TspGWI	ACGGA	3 cut(s)	260, 812, 2254
TspRI	CASTG	4 cut(s)	640, 693, 1531, 2425
Van91I	CCANNNNNTGG	3 cut(s)	467, 908, 1498
Vha464I	CTTAAG	1 cut(s)	1733
VpaK11BI	GGWCC	2 cut(s)	84, 2522
XagI	CCTNNNNNAGG	1 cut(s)	2529
XapI	RAATTY	6 cut(s)	201, 743, 1046, 1999, 2015, 2168
XceI	RCATGY	3 cut(s)	62, 1004, 1300
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1841
XhoI	CTCGAG	2 cut(s)	810, 1338
XmiI	GTMKAC	1 cut(s)	2505
XmnI	GAANNNNTTC	1 cut(s)	151
XspI	CTAG	2 cut(s)	1134, 1170
ZrmI	AGTACT	3 cut(s)	427, 1532, 2429
Zsp2I	ATGCAT	1 cut(s)	1270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.