Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0005666

Physical Location & Seq

Genomic Coordinates Forward (+)

31558173 .. 31570083

Gene Structure 11911 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024081

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2541 bp

ATGGTCTCCACTCTCTCGGGGATACGCTTCCCTCTTATACCTTCCGGTTTCAAGTCCTCCTTGCATTCCAACTTCAATGGCGGCGATCGGAGGTCCGCCACCCTCTGCCTCTTGCTCGACAACAACTCCTCCTTCTCTCGGAAGATCTTTGCCGGAAAGTCTTCGTATGATTCTGATTCGCCTTCTTCTTTAACTGTTGCTGCTTCAAAGAAAGTTCTTGTTCCTGGAGGTCCGACTGATGGCTCTCCACCTTTGACAGAGCAATTGGAAGCTCCCAGCACAGATCCTGAGGTGCTTGAGGATGTAGACAGTGTAACTATGGAAGATGAGATTGTTGAAGATGAAGCAATTTCTTCGTTAGACGTGGGCAGTGTTGATGATGATAAAACCAAGGTTGAGGAAACACTTAGTCCTTTAAAAGGGACAGCAAGTACTGCTCCAAAGTCAATTCCACCACCTGGCACTGGGCAAAAAATATATAAAATAGATCCACTTTTGAAGGGCTTCCGTGATCATCTGGATTACCGATATGGACAATACAAAAGGTTGCGTGCGGAAATTGACAAGTATGAAGGTGGTTTGGAGGTGTTTTCTCGTGGCTATGAGAAGTTTGGGTTCACACGCAGTGCCACAGGTATCACTTATAGGGAGTGGGCACCTGGAGCTAAGTCCGCATCATTAATTGGAGATTTCAACAATTGGAATATGAATGCGGATGTGATGACTCGGAATGAATTTGGGGTCTGGGAGATCTTTTTGCCAAACAATGCAGATGGTTCACCCGCAATTCCGCATGGTTCTCGAGTGAAGATCCGTATGGATACTCCTTCAGGTGTCAAAGACTCAATTCCTGCTTGGATCAAGTTTTCGGTACAGGCTCCTAATGAAATCCCATACAATGGAATATACTATGATCCTCCAGAAGAGGAGAAGTATGTATTTCAACATCCTCAGCCAAAGAGACCACAATCGCTCCGCATTTATGAGGCACATGTTGGAATGAGTAGCACAGAGCCAAAAATTAACACATATGCTGAATTCAGAGATGATGTGCTACCACGCATTAAAAAGCTTGGTTACAACGCTGTTCAGCTCATGGCTATTCAGGAGCATTCCTACTATGCCAGCTTTGGGTACCATGTCACCAATTTTTTTGCACCTAGCAGCCGATGTGGAACGCCTGACGATCTGAAATCTCTGATAGATAGAGCTCATGAACTTGGTCTGCTTGTTCTCATGGATATTGTTCACAGCCATGCATCAAATAATACGTTGGATGGGCTGAACATGTTTGATGGAACCGATAGTCACTACTTCCACTCTGGGTCCCGAGGATATCATTGGATGTGGGATTCTCGCCTTTTCAATTATGGAAGTTGGGAGGTTTTGAGGTATCTTCTCTCAAATGCTAGGTGGTGGTTGGAAGAATTTAAGTTTGACGGGTTCAGATTTGATGGTGTAACTTCAATGATGTACACTCATCATGGATTGCAGGTAGCATTTACTGGAAACTACAGCGAGTATTTTGGATTAGCAACAGATGTAGAGGCTGTCACTTACCTGATGCTGGTTAATGATTTGATTCATGGACTCTACCCTGAAGCTGTTAGCATTGGTGAAGATGTTAGTGGAATGCCAGCATTTTGTATTCCTGTTGCAGATGGCGGTGTTGGATTCGATTATCGCCTGCATATGGCTATTGCTGATAAATGGATTGAGCTCCTCCAGAAAATGGATGAAACCTGGCAAATGGGTGATATTGTTCACACACTGACCAACAGAAGATGGGGAGAGAAGTGTGTTGCTTATGCTGAAAGTCATGACCAAGCTCTTGTTGGTGACAAAACAATTGCATTCTGGTTGATGGACAAGGATATGTATGATTTCATGGCTCTGGATAGACGATCAACTCCCACTATAGATCGTGGAATTGCGTTACACAAAATGATTAGGCTTATTACTATGGGATTAGGTGGAGAAGGATATCTGAATTTTATGGGGAATGAATTTGGACATCCTGAGTGGATTGATTTTCCAAGAGGGGTTCAACATCTTCCAAATGGAAAAATAGTTCCTGGGAACAATAACAGTTTTGACAAATGCCGGCGTAGATTTGATCTTGCAGATGCTGAATATTTAAGATATCATGGCTTGCAAGAATTTGATCGAGCAATGCATCATCTTGAAGAAACCTATAGTTTCATGACTTCTGAGCACCAATATATATCCCGAAAGAATGAAGGAGATAAGGTTATTGTCTTTGAAAGGGGAAACCTGGTTTTTGTCTTCAATTTTCATTGGAGTAGAAGCTATTCAGACTACAGAGTAGGCTGCTTAAAGCCAGGAAAGTATAAGATTGTCTTGGATTCAGATGAGAAAATATTTGGAGGCTTCGATAGGATTGCTCACACCGCGGAATACTTCACAGTTGATGGATGGTATGATGAGCGGCCTAATTCCTTCCAAGTATATGCACCTTGTAGAACAGCTGTGATCTATGCTCCAGTAGAGACAAAGGAAACGATAGCCATCAAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010021 GO:0010033 GO:0016740 GO:0016757 GO:0016758 GO:0019725 GO:0033500 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0042593 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071326 GO:0071329 GO:0071331 GO:0071332 GO:0071333 GO:0071478 GO:0071482 GO:0071704 GO:0104004 GO:1901135 GO:1901137 GO:1901576 GO:1901700 GO:1901701 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

847

Amino Acids

95.84

Weight (kDa)

5.48

Isoelectric Point (pI)

36.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	203 - 286	1e-19	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	352 - 610	1.5e-10	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	741 - 835	2.2e-24	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36390 AT5G03650
fragaria_vesca	FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120
malus_domestica	MD12G1020600.v1.1 MD14G1017700.v1.1
prunus_persica	Prupe.7G115400_v2.0.a1 Prupe.7G115400_v2.0.a1
pyrus_communis	pycom12g01930 pycom14g01610
rosa_chinensis	RchiOBHm_Chr3g0472421
rosa_laevigata	RLG00000024081
rosa_multiflora	Rmu_co8370739.1_g000001 Rmu_sc0001096.1_g000015
rosa_roxburghii	Rroxscaffold_6G00408930
rosa_rugosa	Rorug03G0124700 Rorug03G0124700 Rorug03G0124800 Rorug03G0124900
rosa_samantha	Rh3AG177100 Rh3BG203100 Rh3CG193400 Rh3CG200600 Rh3DG172000
rosa_wichuraiana	Rw3G016440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1483
Acc65I	GGTACC	1 cut(s)	1134
AccB1I	GGYRCC	2 cut(s)	655, 1134
AccB7I	CCANNNNNTGG	3 cut(s)	458, 899, 1732
AccBSI	CCGCTC	1 cut(s)	2449
AccI	GTMKAC	1 cut(s)	306
AccII	CGCG	1 cut(s)	2414
AclWI	GGATC	5 cut(s)	278, 482, 805, 866, 908
AcsI	RAATTY	6 cut(s)	734, 1037, 1427, 1990, 2006, 2159
AcuI	CTGAAG	2 cut(s)	813, 1620
AdeI	CACNNNGTG	1 cut(s)	626
AfaI	GTAC	4 cut(s)	433, 873, 1136, 1475
AflIII	ACRYGT	2 cut(s)	991, 1287
AjiI	CACGTC	1 cut(s)	364
AjnI	CCWGG	7 cut(s)	223, 457, 658, 1742, 2074, 2274, 2341
AloI	GAACNNNNNNTCC	2 cut(s)	2055, 2087
Alw21I	GWGCWC	3 cut(s)	1213, 1722, 2217
Alw26I	GTCTC	3 cut(s)	10, 955, 2504
AlwI	GGATC	5 cut(s)	278, 482, 805, 866, 908
AlwNI	CAGNNNCTG	2 cut(s)	287, 2129
Ama87I	CYCGRG	3 cut(s)	16, 801, 1329
AoxI	GGCC	1 cut(s)	2450
ApeKI	GCWGC	3 cut(s)	200, 1164, 2331
ApoI	RAATTY	6 cut(s)	734, 1037, 1427, 1990, 2006, 2159
AseI	ATTAAT	1 cut(s)	680
Asp700I	GAANNNNTTC	3 cut(s)	160, 503, 2311
Asp718I	GGTACC	1 cut(s)	1134
AspS9I	GGNCC	3 cut(s)	93, 230, 1326
AsuHPI	GGTGA	5 cut(s)	771, 1135, 1628, 1766, 1850
AvaI	CYCGRG	3 cut(s)	16, 801, 1329
AvaII	GGWCC	3 cut(s)	93, 230, 1326
AxyI	CCTNAGG	1 cut(s)	288
BaeGI	GKGCMC	1 cut(s)	658
BanI	GGYRCC	2 cut(s)	655, 1134
BanII	GRGCYC	2 cut(s)	1213, 1722
BauI	CACGAG	1 cut(s)	594
BbsI	GAAGAC	2 cut(s)	153, 2278
Bbv12I	GWGCWC	3 cut(s)	1213, 1722, 2217
BbvCI	CCTCAGC	1 cut(s)	951
BbvI	GCAGC	3 cut(s)	187, 1176, 2318
BcgI	CGANNNNNNTGC	6 cut(s)	336, 370, 782, 816, 2384, 2418
BciT130I	CCWGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
BciVI	GTATCC	2 cut(s)	15, 814
BclI	TGATCA	1 cut(s)	511
BcoDI	GTCTC	3 cut(s)	10, 955, 2504
BfaI	CTAG	2 cut(s)	1161, 1410
BfmI	CTRYAG	4 cut(s)	1513, 1917, 2194, 2320
BfuAI	ACCTGC	1 cut(s)	1483
BfuI	GTATCC	2 cut(s)	15, 814
BglII	AGATCT	2 cut(s)	144, 750
BisI	GCNGC	5 cut(s)	82, 201, 1165, 2332, 2450
BlsI	GCNGC	5 cut(s)	83, 202, 1166, 2333, 2451
BmcAI	AGTACT	1 cut(s)	433
Bme1390I	CCNGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
Bme18I	GGWCC	3 cut(s)	93, 230, 1326
BmeT110I	CYCGRG	3 cut(s)	16, 801, 1329
BmgBI	CACGTC	1 cut(s)	364
BmgT120I	GGNCC	3 cut(s)	93, 230, 1326
BmiI	GGNNCC	6 cut(s)	657, 879, 1136, 1300, 1327, 1328
BmrFI	CCNGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
BmrI	ACTGGG	1 cut(s)	474
BmsI	GCATC	5 cut(s)	683, 1268, 1554, 2116, 2185
BmuI	ACTGGG	1 cut(s)	474
BpiI	GAAGAC	2 cut(s)	153, 2278
BpmI	CTGGAG	5 cut(s)	246, 681, 903, 1709, 2487
Bpu10I	CCTNAGC	1 cut(s)	951
BpuEI	CTTGAG	1 cut(s)	317
BsaBI	GATNNNNATC	1 cut(s)	2528
BsaI	GGTCTC	2 cut(s)	10, 955
BsaJI	CCNNGG	4 cut(s)	390, 1330, 2075, 2412
BsaWI	WCCGGW	1 cut(s)	44
BsaXI	ACNNNNNCTCC	8 cut(s)	110, 113, 140, 143, 957, 987, 2235, 2265
Bse118I	RCCGGY	1 cut(s)	2103
Bse1I	ACTGG	3 cut(s)	469, 1510, 2504
Bse21I	CCTNAGG	1 cut(s)	288
Bse3DI	GCAATG	1 cut(s)	2178
Bse8I	GATNNNNATC	1 cut(s)	2528
BseBI	CCWGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
BseDI	CCNNGG	4 cut(s)	390, 1330, 2075, 2412
BseGI	GGATG	8 cut(s)	307, 721, 946, 1282, 1350, 1741, 2014, 2441
BseJI	GATNNNNATC	1 cut(s)	2528
BseMI	GCAATG	1 cut(s)	2178
BseMII	CTCAG	4 cut(s)	279, 965, 2010, 2202
BseNI	ACTGG	3 cut(s)	469, 1510, 2504
BseRI	GAGGAG	3 cut(s)	118, 941, 1712
BseSI	GKGCMC	1 cut(s)	658
BseXI	GCAGC	3 cut(s)	187, 1176, 2318
BseYI	CCCAGC	1 cut(s)	275
Bsh1236I	CGCG	1 cut(s)	2414
Bsh1285I	CGRYCG	1 cut(s)	88
BshFI	GGCC	1 cut(s)	2452
BshNI	GGYRCC	2 cut(s)	655, 1134
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	3 cut(s)	1213, 1722, 2217
BsiHKCI	CYCGRG	3 cut(s)	16, 801, 1329
BsiSI	CCGG	3 cut(s)	45, 153, 2104
BslFI	GGGAC	2 cut(s)	436, 1312
BsmAI	GTCTC	3 cut(s)	10, 955, 2504
BsmFI	GGGAC	2 cut(s)	436, 1312
BsmI	GAATGC	5 cut(s)	64, 715, 1111, 1638, 1853
BsnI	GGCC	1 cut(s)	2452
Bso31I	GGTCTC	2 cut(s)	10, 955
BsoBI	CYCGRG	3 cut(s)	16, 801, 1329
Bsp1286I	GDGCHC	4 cut(s)	658, 1213, 1722, 2217
Bsp1407I	TGTACA	1 cut(s)	1473
BspANI	GGCC	1 cut(s)	2452
BspCNI	CTCAG	4 cut(s)	280, 964, 2011, 2203
BspFNI	CGCG	1 cut(s)	2414
BspHI	TCATGA	3 cut(s)	1213, 1819, 2202
BspLI	GGNNCC	6 cut(s)	657, 879, 1136, 1300, 1327, 1328
BspMI	ACCTGC	1 cut(s)	1483
BspPI	GGATC	5 cut(s)	278, 482, 805, 866, 908
BspT107I	GGYRCC	2 cut(s)	655, 1134
BspTNI	GGTCTC	2 cut(s)	10, 955
BsrBI	CCGCTC	1 cut(s)	2449
BsrDI	GCAATG	1 cut(s)	2178
BsrFI	RCCGGY	1 cut(s)	2103
BsrGI	TGTACA	1 cut(s)	1473
BsrI	ACTGG	3 cut(s)	469, 1510, 2504
BssAI	RCCGGY	1 cut(s)	2103
BssECI	CCNNGG	4 cut(s)	390, 1330, 2075, 2412
BssSI	CACGAG	1 cut(s)	594
BssT1I	CCWWGG	1 cut(s)	390
Bst2BI	CACGAG	1 cut(s)	594
Bst2UI	CCWGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
Bst4CI	ACNGT	4 cut(s)	196, 311, 2090, 2428
Bst6I	CTCTTC	1 cut(s)	918
BstAPI	GCANNNNNTGC	1 cut(s)	434
BstAUI	TGTACA	1 cut(s)	1473
BstC8I	GCNNGC	6 cut(s)	552, 1126, 1638, 1688, 2105, 2153
BstDEI	CTNAG	6 cut(s)	288, 407, 666, 951, 2019, 2211
BstDSI	CCRYGG	1 cut(s)	2412
BstENI	CCTNNNNNAGG	1 cut(s)	417
BstF5I	GGATG	8 cut(s)	307, 721, 946, 1282, 1350, 1741, 2014, 2441
BstFNI	CGCG	1 cut(s)	2414
BstMAI	GTCTC	3 cut(s)	10, 955, 2504
BstMCI	CGRYCG	1 cut(s)	88
BstMWI	GCNNNNNNNGC	4 cut(s)	434, 662, 671, 2411
BstNI	CCWGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
BstNSI	RCATGY	2 cut(s)	995, 1291
BstSCI	CCNGG	7 cut(s)	223, 457, 658, 1742, 2074, 2274, 2341
BstSFI	CTRYAG	4 cut(s)	1513, 1917, 2194, 2320
BstSLI	GKGCMC	1 cut(s)	658
BstUI	CGCG	1 cut(s)	2414
BstV1I	GCAGC	3 cut(s)	187, 1176, 2318
BstV2I	GAAGAC	2 cut(s)	153, 2278
BstX2I	RGATCY	5 cut(s)	144, 283, 487, 750, 810
BstYI	RGATCY	5 cut(s)	144, 283, 487, 750, 810
Bsu36I	CCTNAGG	1 cut(s)	288
BsuI	GTATCC	2 cut(s)	15, 814
BsuRI	GGCC	1 cut(s)	2452
BtgI	CCRYGG	1 cut(s)	2412
BtrI	CACGTC	1 cut(s)	364
BtsCI	GGATG	8 cut(s)	307, 721, 946, 1282, 1350, 1741, 2014, 2441
BtsI	GCAGTG	2 cut(s)	376, 631
BtsIMutI	CAGTG	5 cut(s)	316, 376, 462, 631, 1769
BveI	ACCTGC	1 cut(s)	1483
Cac8I	GCNNGC	6 cut(s)	552, 1126, 1638, 1688, 2105, 2153
CaiI	CAGNNNCTG	2 cut(s)	287, 2129
CciI	TCATGA	3 cut(s)	1213, 1819, 2202
Cfr10I	RCCGGY	1 cut(s)	2103
Cfr13I	GGNCC	3 cut(s)	93, 230, 1326
Cfr42I	CCGCGG	1 cut(s)	2415
CsiI	ACCWGGT	1 cut(s)	2274
Csp6I	GTAC	4 cut(s)	432, 872, 1135, 1474
CviQI	GTAC	4 cut(s)	432, 872, 1135, 1474
DdeI	CTNAG	6 cut(s)	288, 407, 666, 951, 2019, 2211
DraI	TTTAAA	1 cut(s)	417
DraIII	CACNNNGTG	1 cut(s)	626
Eam1104I	CTCTTC	1 cut(s)	918
EarI	CTCTTC	1 cut(s)	918
EciI	GGCGGA	1 cut(s)	85
Ecl136II	GAGCTC	2 cut(s)	1211, 1720
Eco130I	CCWWGG	1 cut(s)	390
Eco24I	GRGCYC	2 cut(s)	1213, 1722
Eco31I	GGTCTC	2 cut(s)	10, 955
Eco32I	GATATC	3 cut(s)	1337, 1985, 2144
Eco47I	GGWCC	3 cut(s)	93, 230, 1326
Eco53kI	GAGCTC	2 cut(s)	1211, 1720
Eco57I	CTGAAG	2 cut(s)	813, 1620
Eco81I	CCTNAGG	1 cut(s)	288
Eco88I	CYCGRG	3 cut(s)	16, 801, 1329
EcoICRI	GAGCTC	2 cut(s)	1211, 1720
EcoNI	CCTNNNNNAGG	1 cut(s)	417
EcoO109I	RGGNCCY	1 cut(s)	1326
EcoRI	GAATTC	1 cut(s)	1037
EcoRII	CCWGG	7 cut(s)	223, 457, 658, 1742, 2074, 2274, 2341
EcoRV	GATATC	3 cut(s)	1337, 1985, 2144
EcoT14I	CCWWGG	1 cut(s)	390
EcoT22I	ATGCAT	2 cut(s)	1261, 2178
EcoT38I	GRGCYC	2 cut(s)	1213, 1722
ErhI	CCWWGG	1 cut(s)	390
FalI	AAGNNNNNCTT	4 cut(s)	44, 76, 2345, 2377
FaqI	GGGAC	2 cut(s)	436, 1312
FauI	CCCGC	1 cut(s)	790
FauNDI	CATATG	2 cut(s)	1030, 1692
FbaI	TGATCA	1 cut(s)	511
FblI	GTMKAC	1 cut(s)	306
Fnu4HI	GCNGC	5 cut(s)	82, 201, 1165, 2332, 2450
FokI	GGATG	8 cut(s)	314, 728, 933, 1289, 1357, 1748, 2001, 2448
FriOI	GRGCYC	2 cut(s)	1213, 1722
Fsp4HI	GCNGC	5 cut(s)	82, 201, 1165, 2332, 2450
FspBI	CTAG	2 cut(s)	1161, 1410
GluI	GCNGC	5 cut(s)	82, 201, 1165, 2332, 2450
GsaI	CCCAGC	1 cut(s)	279
GsuI	CTGGAG	5 cut(s)	246, 681, 903, 1709, 2487
HaeIII	GGCC	1 cut(s)	2452
HapII	CCGG	3 cut(s)	45, 153, 2104
HindIII	AAGCTT	1 cut(s)	1070
HinfI	GANTC	9 cut(s)	170, 176, 724, 842, 1352, 1582, 1590, 1674, 2366
HpaII	CCGG	3 cut(s)	45, 153, 2104
HphI	GGTGA	5 cut(s)	771, 1135, 1628, 1766, 1850
Hpy166II	GTNNAC	6 cut(s)	307, 618, 779, 1249, 1476, 1765
Hpy8I	GTNNAC	6 cut(s)	307, 618, 779, 1249, 1476, 1765
HpyAV	CCTTC	9 cut(s)	51, 142, 192, 493, 566, 837, 1973, 2234, 2470
HpyCH4III	ACNGT	4 cut(s)	196, 311, 2090, 2428
HpyCH4IV	ACGT	2 cut(s)	363, 1271
HpyF10VI	GCNNNNNNNGC	4 cut(s)	434, 662, 671, 2411
HpyF3I	CTNAG	6 cut(s)	288, 407, 666, 951, 2019, 2211
HpySE526I	ACGT	2 cut(s)	363, 1271
KflI	GGGWCCC	1 cut(s)	1326
KpnI	GGTACC	1 cut(s)	1138
KroI	GCCGGC	1 cut(s)	2103
KroNI	GCCGGC	1 cut(s)	2105
Ksp22I	TGATCA	1 cut(s)	511
KspI	CCGCGG	1 cut(s)	2415
LmnI	GCTCC	8 cut(s)	277, 442, 662, 883, 978, 1108, 1725, 2506
Lsp1109I	GCAGC	3 cut(s)	187, 1176, 2318
LweI	GCATC	5 cut(s)	683, 1268, 1554, 2116, 2185
MabI	ACCWGGT	1 cut(s)	2274
MaeI	CTAG	2 cut(s)	1161, 1410
MaeII	ACGT	2 cut(s)	363, 1271
MaeIII	GTNAC	8 cut(s)	313, 1076, 1141, 1307, 1459, 1552, 1838, 1935
MbiI	CCGCTC	1 cut(s)	2449
MfeI	CAATTG	3 cut(s)	263, 697, 1848
MflI	RGATCY	5 cut(s)	144, 283, 487, 750, 810
MhlI	GDGCHC	4 cut(s)	658, 1213, 1722, 2217
MlyI	GAGTC	3 cut(s)	718, 836, 1584
MmeI	TCCRAC	6 cut(s)	93, 257, 976, 1254, 1401, 1651
Mph1103I	ATGCAT	2 cut(s)	1261, 2178
MroNI	GCCGGC	1 cut(s)	2103
MroXI	GAANNNNTTC	3 cut(s)	160, 503, 2311
MseI	TTAA	9 cut(s)	191, 416, 680, 1023, 1065, 1431, 1572, 2138, 2336
MslI	CAYNNNNRTG	1 cut(s)	1254
MspA1I	CMGCKG	2 cut(s)	2414, 2489
MspI	CCGG	3 cut(s)	45, 153, 2104
MspR9I	CCNGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
MunI	CAATTG	3 cut(s)	263, 697, 1848
Mva1269I	GAATGC	5 cut(s)	64, 715, 1111, 1638, 1853
MvaI	CCWGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
MvnI	CGCG	1 cut(s)	2414
MwoI	GCNNNNNNNGC	4 cut(s)	434, 662, 671, 2411
NaeI	GCCGGC	1 cut(s)	2105
NdeI	CATATG	2 cut(s)	1030, 1692
NgoMIV	GCCGGC	1 cut(s)	2103
NlaIV	GGNNCC	6 cut(s)	657, 879, 1136, 1300, 1327, 1328
NmuCI	GTSAC	4 cut(s)	1141, 1307, 1552, 1838
NsiI	ATGCAT	2 cut(s)	1261, 2178
NspI	RCATGY	2 cut(s)	995, 1291
PaeR7I	CTCGAG	1 cut(s)	801
PagI	TCATGA	3 cut(s)	1213, 1819, 2202
PciI	ACATGT	2 cut(s)	991, 1287
PctI	GAATGC	5 cut(s)	64, 715, 1111, 1638, 1853
PdiI	GCCGGC	1 cut(s)	2105
PdmI	GAANNNNTTC	3 cut(s)	160, 503, 2311
PfeI	GAWTC	6 cut(s)	170, 176, 1352, 1582, 1674, 2366
PflMI	CCANNNNNTGG	3 cut(s)	458, 899, 1732
PfoI	TCCNGGA	1 cut(s)	223
PkrI	GCNGC	5 cut(s)	83, 202, 1166, 2333, 2451
Ple19I	CGATCG	1 cut(s)	88
PleI	GAGTC	3 cut(s)	718, 836, 1584
PpsI	GAGTC	3 cut(s)	718, 836, 1584
PpuMI	RGGWCCY	1 cut(s)	1326
PscI	ACATGT	2 cut(s)	991, 1287
PshBI	ATTAAT	1 cut(s)	680
Psp124BI	GAGCTC	2 cut(s)	1213, 1722
Psp5II	RGGWCCY	1 cut(s)	1326
Psp6I	CCWGG	7 cut(s)	223, 457, 658, 1742, 2074, 2274, 2341
PspFI	CCCAGC	1 cut(s)	275
PspGI	CCWGG	7 cut(s)	223, 457, 658, 1742, 2074, 2274, 2341
PspN4I	GGNNCC	6 cut(s)	657, 879, 1136, 1300, 1327, 1328
PspPI	GGNCC	3 cut(s)	93, 230, 1326
PspPPI	RGGWCCY	1 cut(s)	1326
PstNI	CAGNNNCTG	2 cut(s)	287, 2129
PsuI	RGATCY	5 cut(s)	144, 283, 487, 750, 810
PvuI	CGATCG	1 cut(s)	88
PvuII	CAGCTG	1 cut(s)	2489
RsaI	GTAC	4 cut(s)	433, 873, 1136, 1475
RsaNI	GTAC	4 cut(s)	432, 872, 1135, 1474
RseI	CAYNNNNRTG	1 cut(s)	1254
SacI	GAGCTC	2 cut(s)	1213, 1722
SacII	CCGCGG	1 cut(s)	2415
SaqAI	TTAA	9 cut(s)	191, 416, 680, 1023, 1065, 1431, 1572, 2138, 2336
SatI	GCNGC	5 cut(s)	82, 201, 1165, 2332, 2450
Sau96I	GGNCC	3 cut(s)	93, 230, 1326
ScaI	AGTACT	1 cut(s)	433
SchI	GAGTC	3 cut(s)	718, 836, 1584
ScrFI	CCNGG	7 cut(s)	225, 459, 660, 1744, 2076, 2276, 2343
SduI	GDGCHC	4 cut(s)	658, 1213, 1722, 2217
SexAI	ACCWGGT	1 cut(s)	2274
SfaNI	GCATC	5 cut(s)	683, 1268, 1554, 2116, 2185
SfcI	CTRYAG	4 cut(s)	1513, 1917, 2194, 2320
Sfr274I	CTCGAG	1 cut(s)	801
Sfr303I	CCGCGG	1 cut(s)	2415
SgrBI	CCGCGG	1 cut(s)	2415
SinI	GGWCC	3 cut(s)	93, 230, 1326
SlaI	CTCGAG	1 cut(s)	801
SmiMI	CAYNNNNRTG	1 cut(s)	1254
SmlI	CTYRAG	2 cut(s)	296, 801
SmoI	CTYRAG	2 cut(s)	296, 801
SspI	AATATT	2 cut(s)	2135, 2382
SspMI	CTAG	2 cut(s)	1161, 1410
SstI	GAGCTC	2 cut(s)	1213, 1722
StyD4I	CCNGG	7 cut(s)	223, 457, 658, 1742, 2074, 2274, 2341
StyI	CCWWGG	1 cut(s)	390
TaaI	ACNGT	4 cut(s)	196, 311, 2090, 2428
TaiI	ACGT	2 cut(s)	366, 1274
TaqI	TCGA	5 cut(s)	117, 802, 1677, 2167, 2394
TatI	WGTACW	2 cut(s)	431, 1473
TauI	GCSGC	2 cut(s)	84, 2452
TfiI	GAWTC	6 cut(s)	170, 176, 1352, 1582, 1674, 2366
Tru1I	TTAA	9 cut(s)	191, 416, 680, 1023, 1065, 1431, 1572, 2138, 2336
Tru9I	TTAA	9 cut(s)	191, 416, 680, 1023, 1065, 1431, 1572, 2138, 2336
TscAI	CASTG	5 cut(s)	316, 376, 469, 631, 1776
TseFI	GTSAC	4 cut(s)	1141, 1307, 1552, 1838
TseI	GCWGC	3 cut(s)	200, 1164, 2331
Tsp45I	GTSAC	4 cut(s)	1141, 1307, 1552, 1838
TspGWI	ACGGA	2 cut(s)	497, 803
TspRI	CASTG	5 cut(s)	316, 376, 469, 631, 1776
Van91I	CCANNNNNTGG	3 cut(s)	458, 899, 1732
VpaK11BI	GGWCC	3 cut(s)	93, 230, 1326
VspI	ATTAAT	1 cut(s)	680
XagI	CCTNNNNNAGG	1 cut(s)	417
XapI	RAATTY	6 cut(s)	734, 1037, 1427, 1990, 2006, 2159
XceI	RCATGY	2 cut(s)	995, 1291
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1832
XhoI	CTCGAG	1 cut(s)	801
XmiI	GTMKAC	1 cut(s)	306
XmnI	GAANNNNTTC	3 cut(s)	160, 503, 2311
XspI	CTAG	2 cut(s)	1161, 1410
ZrmI	AGTACT	1 cut(s)	433
Zsp2I	ATGCAT	2 cut(s)	1261, 2178

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.