Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0005666

Physical Location & Seq

Genomic Coordinates Reverse (-)

17178084 .. 17190005

Gene Structure 11922 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG193400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGGTCTCGACTCTCTCGGGGATACGCTTCCCTCTTATACCTTCCGGTTTCAAGTCCTCCCTGCATTCCAACTTCAATGGCGGCGATCGGAGGTCCACCACCCTCTGCCTCTTGCTCGACAACACCTCCTCCTTCTCTCGGAAGATCTTTGCCGGAAAGTCTTCGTATGATTCTGATTCGCCTTCTTCTTTAACTGTTGCTGCTTCAAAGAAAGTTCTTGTTCCTGGAGGTCCGACTGATGGCTCTCCACCTTTGACAGAGCAATTGGAAGCTCCCAGCACAGATTCAGAGGATCCTCAGGTGCTTGAGGATGTAGACAGTGTAACTATGGAAGATGAGATTGTTGAAGATGAAGTAATTTCTTCATTAGACGTGGGCAGTGTTGATGATGATAAAACCAAGGTTGAGGAAACACCTAGTCCTTTAAAAGGGACAGCAAGTACTGCTCCAAAGTCAATTCCACCACCTGGCACTGGGCAAAAAATATATGAAATAGATCCACTTTTGAAGGGCTTCCGTGATCATTTGGATTACCGATATGGACAATACAAAAAGTTGCGTGTGGAAATTGACAAGTATGAAGGTGGTTTGGAGGTGTTTTCTCGTGGCTATGAGAAGTTTGGGTTCACACGCAGTGCCACAGGTATCACTTATAGGGAGTGGGCACCTGGAGCTAAGTCCGCATCATTAATTGGAGATTTCAACAATTGGAATATGAATGCGGATGTGATGACTCAGAATGAATTTGGGGTCTGGGAAATCTTTTTGCCAAACAATGCAGATGGTTCACCCGCAATTCCGCATGGTTCTCGAGTTAAGATCCGTATGGATACTCCTTCAGGTGTCAAAGACTCAATTCCTGCTTGGATCAAGTTTTCGGTACAGGCTCCTAATGAAATCCCATACAATGGAATATACTATGATCCTCCAGAAGAGGAGAAGTATGTATTTCAACATCCTCAGCCGAAGAGACCACAATCACTCCGCATTTATGAGGCACATGTTGGAATGAGTAGCACAGAGCCAAAAATTAACACATATGCTGAATTCAGAGATGATGTGCTACCACGCATTAAAAAGCTTGGTTACAATGCTGTTCAGCTCATGGCTATTCAGGAGCATTCCTACTATGCCAGCTTTGGGTACCATGTCACCAATTTTTTTGCACCTAGCAGCCGATGTGGAACGCCTGACGATCTGAAATCTCTGATAGATAGAGCTCATGAACTTGGTCTGCTTGTACTCATGGATATTGTTCACAGCCATGCATCAAATAATACGTTGGATGGGCTGAACATGTTTGATGGAACCGATAGTCACTACTTCCACTCTGGGTCCCGAGGATATCATTGGATGTGGGATTCTCGCCTTTTCAATTATGGAAGTTGGGAGGTTTTGAGGTATCTTCTCTCAAATGCAAGGTGGTGGTTGGAAGAATTTAAGTTTGACGGGTTCAGATTTGATGGTGTAACTTCAATGATGTACACTCATCATGGATTGCAGGTAGCATTTACCGGAAACTACAGCGAGTATTTTGGATTAGCAACAGATGTAGAGGCTGTCACTTACCTGATGCTGGTTAATGATTTGATTCATGGGCTCTACCCTGAAGCTGTTAGCATTGGTGAAGATGTTAGTGGAATGCCAGCATTTTGTATTCCTGTTGCGGGTGGTGGTGTTGGATTTGATTATCGCTTGCATATGGCTATTGCTGATAAATGGATTGAGCTCCTCCAGAAAATGGATGAAACCTGGCAAATGAGTGATATTGTTCACACACTGACCAACAGAAGATGGGGAGAGAAGTGTGTTGCTTATGCTGAAAGTCATGACCAAGCTCTTGTTGGTGACAAAACAATTGCATTCTGGTTGATGGACAAGGATATGTATGATTTCATGGCTCTGGATAGACCATCAACTCCCACTGTAGATCGTGGAATTGCGTTACACAAAATGATTAGGCTTATTACTATGGGATTAGGTGGAGAAGGATATCTGAATTTTATGGGGAATGAATTTGGACATCCTGAGTGGATTGATTTTCCAAGAGGGGTTCAACATCTTCCAAATGGAAAAATAGTTCCTGGGAACAATAACAGTTTTGACAAATGCCGGCGTAGATTTGATCTTGCAGATGCTGAATATTTAAGATATCATGGCTTGCAAGAATTTGATCGAGCAATGCATCATCTTGAAGAAACCTATAGTTTCATGACTTCTGAGCACCAATATATATCCCGAAAGGATGAAGGAGATAAGGTTATTGTCTTTGAAAGGGGAAACCTGGTCTTTGTCTTCAATTTTCATTGGAGTAAAAGCTATTCAGACTACCGAGTAGGCTGCTTAAAGCCAGGAAAGTATAAGATTGTCTTGGATTCAGATGAGAAAATATTTGGAGGCTTTGATAGGATTGCTCACACCGCGGAGTACTTCACAGTTGATGGATGGTATGATGAGCGGCCTAATTCCTTCCAAGTATATGCACCTTGTAGAACAGCTGTGGTCTATGCTCTAGTAGAGACAAAGGAAACGATACCCATCAAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010021 GO:0010033 GO:0016740 GO:0016757 GO:0016758 GO:0019725 GO:0033500 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0042593 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071326 GO:0071329 GO:0071331 GO:0071332 GO:0071333 GO:0071478 GO:0071482 GO:0071704 GO:0104004 GO:1901135 GO:1901137 GO:1901576 GO:1901700 GO:1901701 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

849

Amino Acids

96.08

Weight (kDa)

5.3

Isoelectric Point (pI)

34.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	206 - 289	1.7e-19	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	355 - 613	1.5e-10	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	744 - 838	5.4e-25	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36390 AT5G03650
fragaria_vesca	FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120
malus_domestica	MD12G1020600.v1.1 MD14G1017700.v1.1
prunus_persica	Prupe.7G115400_v2.0.a1 Prupe.7G115400_v2.0.a1
pyrus_communis	pycom12g01930 pycom14g01610
rosa_chinensis	RchiOBHm_Chr3g0472421
rosa_laevigata	RLG00000024081
rosa_multiflora	Rmu_co8370739.1_g000001 Rmu_sc0001096.1_g000015
rosa_roxburghii	Rroxscaffold_6G00408930
rosa_rugosa	Rorug03G0124700 Rorug03G0124700 Rorug03G0124800 Rorug03G0124900
rosa_samantha	Rh3AG177100 Rh3BG203100 Rh3CG193400 Rh3CG200600 Rh3DG172000
rosa_wichuraiana	Rw3G016440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1492
Acc65I	GGTACC	1 cut(s)	1143
AccB1I	GGYRCC	2 cut(s)	664, 1143
AccB7I	CCANNNNNTGG	3 cut(s)	467, 908, 1741
AccBSI	CCGCTC	1 cut(s)	2458
AccI	GTMKAC	1 cut(s)	315
AccII	CGCG	1 cut(s)	2423
AclWI	GGATC	6 cut(s)	287, 300, 491, 814, 875, 917
AcsI	RAATTY	6 cut(s)	743, 1046, 1436, 1999, 2015, 2168
AcuI	CTGAAG	2 cut(s)	822, 1629
AdeI	CACNNNGTG	1 cut(s)	635
AfaI	GTAC	6 cut(s)	442, 882, 1145, 1242, 1484, 2429
AflIII	ACRYGT	2 cut(s)	1000, 1296
AjiI	CACGTC	1 cut(s)	373
AjnI	CCWGG	7 cut(s)	223, 466, 667, 1751, 2083, 2283, 2350
AloI	GAACNNNNNNTCC	2 cut(s)	2064, 2096
Alw21I	GWGCWC	3 cut(s)	1222, 1731, 2226
Alw26I	GTCTC	3 cut(s)	10, 964, 2513
AlwI	GGATC	6 cut(s)	287, 300, 491, 814, 875, 917
AlwNI	CAGNNNCTG	1 cut(s)	2138
Ama87I	CYCGRG	3 cut(s)	16, 810, 1338
AoxI	GGCC	1 cut(s)	2459
ApeKI	GCWGC	3 cut(s)	200, 1173, 2340
ApoI	RAATTY	6 cut(s)	743, 1046, 1436, 1999, 2015, 2168
AseI	ATTAAT	1 cut(s)	689
Asp700I	GAANNNNTTC	2 cut(s)	160, 512
Asp718I	GGTACC	1 cut(s)	1143
AspS9I	GGNCC	3 cut(s)	93, 230, 1335
AsuHPI	GGTGA	4 cut(s)	780, 1144, 1637, 1859
AvaI	CYCGRG	3 cut(s)	16, 810, 1338
AvaII	GGWCC	3 cut(s)	93, 230, 1335
AxyI	CCTNAGG	1 cut(s)	297
BaeGI	GKGCMC	1 cut(s)	667
BamHI	GGATCC	1 cut(s)	292
BanI	GGYRCC	2 cut(s)	664, 1143
BanII	GRGCYC	3 cut(s)	1222, 1602, 1731
BauI	CACGAG	1 cut(s)	603
BbsI	GAAGAC	2 cut(s)	153, 2287
Bbv12I	GWGCWC	3 cut(s)	1222, 1731, 2226
BbvCI	CCTCAGC	1 cut(s)	960
BbvI	GCAGC	3 cut(s)	187, 1185, 2327
BcgI	CGANNNNNNTGC	4 cut(s)	791, 825, 2322, 2356
BciT130I	CCWGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
BciVI	GTATCC	2 cut(s)	15, 823
BclI	TGATCA	1 cut(s)	520
BcoDI	GTCTC	3 cut(s)	10, 964, 2513
BfaI	CTAG	3 cut(s)	417, 1170, 2513
BfmI	CTRYAG	3 cut(s)	1522, 1926, 2203
BfuAI	ACCTGC	1 cut(s)	1492
BfuI	GTATCC	2 cut(s)	15, 823
BglII	AGATCT	1 cut(s)	144
BisI	GCNGC	5 cut(s)	82, 201, 1174, 2341, 2459
BlsI	GCNGC	5 cut(s)	83, 202, 1175, 2342, 2460
BmcAI	AGTACT	2 cut(s)	442, 2429
Bme1390I	CCNGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
Bme18I	GGWCC	3 cut(s)	93, 230, 1335
BmeT110I	CYCGRG	3 cut(s)	16, 810, 1338
BmgBI	CACGTC	1 cut(s)	373
BmgT120I	GGNCC	3 cut(s)	93, 230, 1335
BmiI	GGNNCC	7 cut(s)	294, 666, 888, 1145, 1309, 1336, 1337
BmrFI	CCNGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
BmrI	ACTGGG	1 cut(s)	483
BmsI	GCATC	5 cut(s)	692, 1277, 1563, 2125, 2194
BmuI	ACTGGG	1 cut(s)	483
BpiI	GAAGAC	2 cut(s)	153, 2287
BpmI	CTGGAG	4 cut(s)	246, 690, 912, 1718
Bpu10I	CCTNAGC	1 cut(s)	960
BpuEI	CTTGAG	1 cut(s)	326
BsaBI	GATNNNNATC	1 cut(s)	2537
BsaI	GGTCTC	2 cut(s)	10, 964
BsaJI	CCNNGG	4 cut(s)	399, 1339, 2084, 2421
BsaWI	WCCGGW	2 cut(s)	44, 1514
BsaXI	ACNNNNNCTCC	8 cut(s)	110, 113, 140, 143, 966, 996, 2244, 2274
Bse118I	RCCGGY	1 cut(s)	2112
Bse1I	ACTGG	1 cut(s)	478
Bse21I	CCTNAGG	1 cut(s)	297
Bse3DI	GCAATG	1 cut(s)	2187
Bse8I	GATNNNNATC	1 cut(s)	2537
BseBI	CCWGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
BseDI	CCNNGG	4 cut(s)	399, 1339, 2084, 2421
BseGI	GGATG	9 cut(s)	316, 730, 955, 1291, 1359, 1750, 2023, 2251, 2450
BseJI	GATNNNNATC	1 cut(s)	2537
BseMI	GCAATG	1 cut(s)	2187
BseMII	CTCAG	5 cut(s)	311, 749, 974, 2019, 2211
BseNI	ACTGG	1 cut(s)	478
BseRI	GAGGAG	3 cut(s)	118, 950, 1721
BseSI	GKGCMC	1 cut(s)	667
BseXI	GCAGC	3 cut(s)	187, 1185, 2327
BseYI	CCCAGC	1 cut(s)	275
Bsh1236I	CGCG	1 cut(s)	2423
Bsh1285I	CGRYCG	1 cut(s)	88
BshFI	GGCC	1 cut(s)	2461
BshNI	GGYRCC	2 cut(s)	664, 1143
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	3 cut(s)	1222, 1731, 2226
BsiHKCI	CYCGRG	3 cut(s)	16, 810, 1338
BsiSI	CCGG	4 cut(s)	45, 153, 1515, 2113
BslFI	GGGAC	2 cut(s)	445, 1321
BsmAI	GTCTC	3 cut(s)	10, 964, 2513
BsmFI	GGGAC	2 cut(s)	445, 1321
BsmI	GAATGC	5 cut(s)	64, 724, 1120, 1647, 1862
BsnI	GGCC	1 cut(s)	2461
Bso31I	GGTCTC	2 cut(s)	10, 964
BsoBI	CYCGRG	3 cut(s)	16, 810, 1338
Bsp1286I	GDGCHC	5 cut(s)	667, 1222, 1602, 1731, 2226
Bsp1407I	TGTACA	1 cut(s)	1482
BspANI	GGCC	1 cut(s)	2461
BspCNI	CTCAG	5 cut(s)	310, 748, 973, 2020, 2212
BspFNI	CGCG	1 cut(s)	2423
BspHI	TCATGA	3 cut(s)	1222, 1828, 2211
BspLI	GGNNCC	7 cut(s)	294, 666, 888, 1145, 1309, 1336, 1337
BspMI	ACCTGC	1 cut(s)	1492
BspPI	GGATC	6 cut(s)	287, 300, 491, 814, 875, 917
BspT107I	GGYRCC	2 cut(s)	664, 1143
BspTNI	GGTCTC	2 cut(s)	10, 964
BsrBI	CCGCTC	1 cut(s)	2458
BsrDI	GCAATG	1 cut(s)	2187
BsrFI	RCCGGY	1 cut(s)	2112
BsrGI	TGTACA	1 cut(s)	1482
BsrI	ACTGG	1 cut(s)	478
BssAI	RCCGGY	1 cut(s)	2112
BssECI	CCNNGG	4 cut(s)	399, 1339, 2084, 2421
BssSI	CACGAG	1 cut(s)	603
BssT1I	CCWWGG	1 cut(s)	399
Bst2BI	CACGAG	1 cut(s)	603
Bst2UI	CCWGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
Bst4CI	ACNGT	5 cut(s)	196, 320, 1927, 2099, 2437
Bst6I	CTCTTC	2 cut(s)	927, 962
BstAPI	GCANNNNNTGC	1 cut(s)	443
BstAUI	TGTACA	1 cut(s)	1482
BstC8I	GCNNGC	5 cut(s)	1135, 1647, 1697, 2114, 2162
BstDEI	CTNAG	6 cut(s)	297, 675, 735, 960, 2028, 2220
BstDSI	CCRYGG	1 cut(s)	2421
BstENI	CCTNNNNNAGG	1 cut(s)	426
BstF5I	GGATG	9 cut(s)	316, 730, 955, 1291, 1359, 1750, 2023, 2251, 2450
BstFNI	CGCG	1 cut(s)	2423
BstMAI	GTCTC	3 cut(s)	10, 964, 2513
BstMCI	CGRYCG	1 cut(s)	88
BstMWI	GCNNNNNNNGC	4 cut(s)	443, 671, 680, 2420
BstNI	CCWGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
BstNSI	RCATGY	2 cut(s)	1004, 1300
BstSCI	CCNGG	7 cut(s)	223, 466, 667, 1751, 2083, 2283, 2350
BstSFI	CTRYAG	3 cut(s)	1522, 1926, 2203
BstSLI	GKGCMC	1 cut(s)	667
BstUI	CGCG	1 cut(s)	2423
BstV1I	GCAGC	3 cut(s)	187, 1185, 2327
BstV2I	GAAGAC	2 cut(s)	153, 2287
BstX2I	RGATCY	4 cut(s)	144, 292, 496, 819
BstYI	RGATCY	4 cut(s)	144, 292, 496, 819
Bsu36I	CCTNAGG	1 cut(s)	297
BsuI	GTATCC	2 cut(s)	15, 823
BsuRI	GGCC	1 cut(s)	2461
BtgI	CCRYGG	1 cut(s)	2421
BtrI	CACGTC	1 cut(s)	373
BtsCI	GGATG	9 cut(s)	316, 730, 955, 1291, 1359, 1750, 2023, 2251, 2450
BtsI	GCAGTG	2 cut(s)	385, 640
BtsIMutI	CAGTG	6 cut(s)	325, 385, 471, 640, 1778, 1923
BveI	ACCTGC	1 cut(s)	1492
Cac8I	GCNNGC	5 cut(s)	1135, 1647, 1697, 2114, 2162
CaiI	CAGNNNCTG	1 cut(s)	2138
CciI	TCATGA	3 cut(s)	1222, 1828, 2211
Cfr10I	RCCGGY	1 cut(s)	2112
Cfr13I	GGNCC	3 cut(s)	93, 230, 1335
Cfr42I	CCGCGG	1 cut(s)	2424
CsiI	ACCWGGT	1 cut(s)	2283
Csp6I	GTAC	6 cut(s)	441, 881, 1144, 1241, 1483, 2428
CviQI	GTAC	6 cut(s)	441, 881, 1144, 1241, 1483, 2428
DdeI	CTNAG	6 cut(s)	297, 675, 735, 960, 2028, 2220
DraI	TTTAAA	1 cut(s)	426
DraIII	CACNNNGTG	1 cut(s)	635
Eam1104I	CTCTTC	2 cut(s)	927, 962
EarI	CTCTTC	2 cut(s)	927, 962
Ecl136II	GAGCTC	2 cut(s)	1220, 1729
Eco130I	CCWWGG	1 cut(s)	399
Eco24I	GRGCYC	3 cut(s)	1222, 1602, 1731
Eco31I	GGTCTC	2 cut(s)	10, 964
Eco32I	GATATC	3 cut(s)	1346, 1994, 2153
Eco47I	GGWCC	3 cut(s)	93, 230, 1335
Eco53kI	GAGCTC	2 cut(s)	1220, 1729
Eco57I	CTGAAG	2 cut(s)	822, 1629
Eco81I	CCTNAGG	1 cut(s)	297
Eco88I	CYCGRG	3 cut(s)	16, 810, 1338
EcoICRI	GAGCTC	2 cut(s)	1220, 1729
EcoNI	CCTNNNNNAGG	1 cut(s)	426
EcoO109I	RGGNCCY	1 cut(s)	1335
EcoRI	GAATTC	1 cut(s)	1046
EcoRII	CCWGG	7 cut(s)	223, 466, 667, 1751, 2083, 2283, 2350
EcoRV	GATATC	3 cut(s)	1346, 1994, 2153
EcoT14I	CCWWGG	1 cut(s)	399
EcoT22I	ATGCAT	2 cut(s)	1270, 2187
EcoT38I	GRGCYC	3 cut(s)	1222, 1602, 1731
ErhI	CCWWGG	1 cut(s)	399
FalI	AAGNNNNNCTT	2 cut(s)	2354, 2386
FaqI	GGGAC	2 cut(s)	445, 1321
FauI	CCCGC	2 cut(s)	799, 1660
FauNDI	CATATG	2 cut(s)	1039, 1701
FbaI	TGATCA	1 cut(s)	520
FblI	GTMKAC	1 cut(s)	315
Fnu4HI	GCNGC	5 cut(s)	82, 201, 1174, 2341, 2459
FokI	GGATG	9 cut(s)	323, 737, 942, 1298, 1366, 1757, 2010, 2258, 2457
FriOI	GRGCYC	3 cut(s)	1222, 1602, 1731
Fsp4HI	GCNGC	5 cut(s)	82, 201, 1174, 2341, 2459
FspBI	CTAG	3 cut(s)	417, 1170, 2513
GluI	GCNGC	5 cut(s)	82, 201, 1174, 2341, 2459
GsaI	CCCAGC	1 cut(s)	279
GsuI	CTGGAG	4 cut(s)	246, 690, 912, 1718
HaeIII	GGCC	1 cut(s)	2461
HapII	CCGG	4 cut(s)	45, 153, 1515, 2113
HindIII	AAGCTT	1 cut(s)	1079
HinfI	GANTC	9 cut(s)	10, 170, 176, 284, 733, 851, 1361, 1591, 2375
HpaII	CCGG	4 cut(s)	45, 153, 1515, 2113
HphI	GGTGA	4 cut(s)	780, 1144, 1637, 1859
Hpy166II	GTNNAC	7 cut(s)	96, 316, 627, 788, 1258, 1485, 1774
Hpy8I	GTNNAC	7 cut(s)	96, 316, 627, 788, 1258, 1485, 1774
HpyAV	CCTTC	9 cut(s)	51, 142, 192, 502, 575, 846, 1982, 2243, 2479
HpyCH4III	ACNGT	5 cut(s)	196, 320, 1927, 2099, 2437
HpyCH4IV	ACGT	2 cut(s)	372, 1280
HpyF10VI	GCNNNNNNNGC	4 cut(s)	443, 671, 680, 2420
HpyF3I	CTNAG	6 cut(s)	297, 675, 735, 960, 2028, 2220
HpySE526I	ACGT	2 cut(s)	372, 1280
KflI	GGGWCCC	1 cut(s)	1335
KpnI	GGTACC	1 cut(s)	1147
KroI	GCCGGC	1 cut(s)	2112
KroNI	GCCGGC	1 cut(s)	2114
Ksp22I	TGATCA	1 cut(s)	520
KspI	CCGCGG	1 cut(s)	2424
LmnI	GCTCC	6 cut(s)	277, 451, 671, 892, 1117, 1734
Lsp1109I	GCAGC	3 cut(s)	187, 1185, 2327
LweI	GCATC	5 cut(s)	692, 1277, 1563, 2125, 2194
MabI	ACCWGGT	1 cut(s)	2283
MaeI	CTAG	3 cut(s)	417, 1170, 2513
MaeII	ACGT	2 cut(s)	372, 1280
MaeIII	GTNAC	8 cut(s)	322, 1085, 1150, 1316, 1468, 1561, 1847, 1944
MbiI	CCGCTC	1 cut(s)	2458
MfeI	CAATTG	3 cut(s)	263, 706, 1857
MflI	RGATCY	4 cut(s)	144, 292, 496, 819
MhlI	GDGCHC	5 cut(s)	667, 1222, 1602, 1731, 2226
MlyI	GAGTC	3 cut(s)	4, 727, 845
MmeI	TCCRAC	6 cut(s)	93, 257, 985, 1263, 1410, 1660
Mph1103I	ATGCAT	2 cut(s)	1270, 2187
MroNI	GCCGGC	1 cut(s)	2112
MroXI	GAANNNNTTC	2 cut(s)	160, 512
MslI	CAYNNNNRTG	1 cut(s)	1263
MspA1I	CMGCKG	2 cut(s)	2423, 2498
MspI	CCGG	4 cut(s)	45, 153, 1515, 2113
MspR9I	CCNGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
MunI	CAATTG	3 cut(s)	263, 706, 1857
Mva1269I	GAATGC	5 cut(s)	64, 724, 1120, 1647, 1862
MvaI	CCWGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
MvnI	CGCG	1 cut(s)	2423
MwoI	GCNNNNNNNGC	4 cut(s)	443, 671, 680, 2420
NaeI	GCCGGC	1 cut(s)	2114
NdeI	CATATG	2 cut(s)	1039, 1701
NgoMIV	GCCGGC	1 cut(s)	2112
NlaIV	GGNNCC	7 cut(s)	294, 666, 888, 1145, 1309, 1336, 1337
NmuCI	GTSAC	4 cut(s)	1150, 1316, 1561, 1847
NsiI	ATGCAT	2 cut(s)	1270, 2187
NspI	RCATGY	2 cut(s)	1004, 1300
PaeR7I	CTCGAG	1 cut(s)	810
PagI	TCATGA	3 cut(s)	1222, 1828, 2211
PciI	ACATGT	2 cut(s)	1000, 1296
PctI	GAATGC	5 cut(s)	64, 724, 1120, 1647, 1862
PdiI	GCCGGC	1 cut(s)	2114
PdmI	GAANNNNTTC	2 cut(s)	160, 512
PfeI	GAWTC	6 cut(s)	170, 176, 284, 1361, 1591, 2375
PflMI	CCANNNNNTGG	3 cut(s)	467, 908, 1741
PfoI	TCCNGGA	1 cut(s)	223
PkrI	GCNGC	5 cut(s)	83, 202, 1175, 2342, 2460
Ple19I	CGATCG	1 cut(s)	88
PleI	GAGTC	3 cut(s)	4, 727, 845
PpsI	GAGTC	3 cut(s)	4, 727, 845
PpuMI	RGGWCCY	1 cut(s)	1335
PscI	ACATGT	2 cut(s)	1000, 1296
PshBI	ATTAAT	1 cut(s)	689
Psp124BI	GAGCTC	2 cut(s)	1222, 1731
Psp5II	RGGWCCY	1 cut(s)	1335
Psp6I	CCWGG	7 cut(s)	223, 466, 667, 1751, 2083, 2283, 2350
PspFI	CCCAGC	1 cut(s)	275
PspGI	CCWGG	7 cut(s)	223, 466, 667, 1751, 2083, 2283, 2350
PspN4I	GGNNCC	7 cut(s)	294, 666, 888, 1145, 1309, 1336, 1337
PspPI	GGNCC	3 cut(s)	93, 230, 1335
PspPPI	RGGWCCY	1 cut(s)	1335
PstNI	CAGNNNCTG	1 cut(s)	2138
PsuI	RGATCY	4 cut(s)	144, 292, 496, 819
PvuI	CGATCG	1 cut(s)	88
PvuII	CAGCTG	1 cut(s)	2498
RsaI	GTAC	6 cut(s)	442, 882, 1145, 1242, 1484, 2429
RsaNI	GTAC	6 cut(s)	441, 881, 1144, 1241, 1483, 2428
RseI	CAYNNNNRTG	1 cut(s)	1263
SacI	GAGCTC	2 cut(s)	1222, 1731
SacII	CCGCGG	1 cut(s)	2424
SatI	GCNGC	5 cut(s)	82, 201, 1174, 2341, 2459
Sau96I	GGNCC	3 cut(s)	93, 230, 1335
ScaI	AGTACT	2 cut(s)	442, 2429
SchI	GAGTC	3 cut(s)	4, 727, 845
ScrFI	CCNGG	7 cut(s)	225, 468, 669, 1753, 2085, 2285, 2352
SduI	GDGCHC	5 cut(s)	667, 1222, 1602, 1731, 2226
SexAI	ACCWGGT	1 cut(s)	2283
SfaNI	GCATC	5 cut(s)	692, 1277, 1563, 2125, 2194
SfcI	CTRYAG	3 cut(s)	1522, 1926, 2203
Sfr274I	CTCGAG	1 cut(s)	810
Sfr303I	CCGCGG	1 cut(s)	2424
SgrBI	CCGCGG	1 cut(s)	2424
SinI	GGWCC	3 cut(s)	93, 230, 1335
SlaI	CTCGAG	1 cut(s)	810
SmiMI	CAYNNNNRTG	1 cut(s)	1263
SmlI	CTYRAG	2 cut(s)	305, 810
SmoI	CTYRAG	2 cut(s)	305, 810
SspI	AATATT	2 cut(s)	2144, 2391
SspMI	CTAG	3 cut(s)	417, 1170, 2513
SstI	GAGCTC	2 cut(s)	1222, 1731
StyD4I	CCNGG	7 cut(s)	223, 466, 667, 1751, 2083, 2283, 2350
StyI	CCWWGG	1 cut(s)	399
TaaI	ACNGT	5 cut(s)	196, 320, 1927, 2099, 2437
TaiI	ACGT	2 cut(s)	375, 1283
TaqI	TCGA	4 cut(s)	8, 117, 811, 2176
TatI	WGTACW	4 cut(s)	440, 1240, 1482, 2427
TauI	GCSGC	2 cut(s)	84, 2461
TfiI	GAWTC	6 cut(s)	170, 176, 284, 1361, 1591, 2375
TscAI	CASTG	6 cut(s)	325, 385, 478, 640, 1785, 1930
TseFI	GTSAC	4 cut(s)	1150, 1316, 1561, 1847
TseI	GCWGC	3 cut(s)	200, 1173, 2340
Tsp45I	GTSAC	4 cut(s)	1150, 1316, 1561, 1847
TspGWI	ACGGA	2 cut(s)	506, 812
TspRI	CASTG	6 cut(s)	325, 385, 478, 640, 1785, 1930
Van91I	CCANNNNNTGG	3 cut(s)	467, 908, 1741
VpaK11BI	GGWCC	3 cut(s)	93, 230, 1335
VspI	ATTAAT	1 cut(s)	689
XagI	CCTNNNNNAGG	1 cut(s)	426
XapI	RAATTY	6 cut(s)	743, 1046, 1436, 1999, 2015, 2168
XceI	RCATGY	2 cut(s)	1004, 1300
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1841
XhoI	CTCGAG	1 cut(s)	810
XmiI	GTMKAC	1 cut(s)	315
XmnI	GAANNNNTTC	2 cut(s)	160, 512
XspI	CTAG	3 cut(s)	417, 1170, 2513
ZrmI	AGTACT	2 cut(s)	442, 2429
Zsp2I	ATGCAT	2 cut(s)	1270, 2187

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.