Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0005666

Physical Location & Seq

Genomic Coordinates Forward (+)

15103955 .. 15116876

Gene Structure 12922 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG172000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGGAAGATGAGATTGTTGAAGATGAAGTAATTTCTTCATTAGATGTGGGCAGTGTTGATGATGATAAAACCAAGGTTGAGGAAACACCTAGTCCTTTAAAAGGGACAGCAAGTACTGCTCCAAAGTCAATTCCACCACCTGGCACTGGGCAAAAAATATATGAAATAGATCCACTTTTGAAGGGCTTCCGTGATCATTTGGATTACCGATATGGACAATACAAAAAGTTGCGTGTGGAAATTGACAAGTATGAAGGTGGTTTGGAGGTGTTTTCTCGTGGCTATGAGAAGTTTGGGTTCACACGCAGTGCCACAGGTATCACTTATAGGGAGTGGGCACCTGGAGCTAAGTCCGCATCATTAATTGGAGATTTCAACAATTGGAATATGAATGCGGATGTGATGACTCAGAATGAATTTGGGGTCTGGGAAATCTTTTTGCCAAACAATGCAGATGGTTCACCCGCAATTCCGCATGGTTCTCGAGTTAAGATCCGTATGGATACTCCTTCAGGTGTCAAAGACTCAATTCCTGCTTGGATCAAGTTTTCGGTACAGGCTCCTAATGAAATCCCATACAATGGAATATACTATGATCCTCCAGAAGAGGAGAAGTATGTATTTCAACATCCTCAGCCGAAGAGACCACAATCACTCCGCATTTATGAGGCACATGTTGGAATGAGTAGCACAGAGCCAAAAATTAACACATATGCTGAATTCAGAGATGATGTGCTACCACGCATTAAAAAGCTTGGTTACAATGCTGTTCAGCTCATGGCTATTCAGGAGCATTCCTACTATGCCAGCTTTGGGTACCATGTCACCAATTTTTTTGCACCTAGCAGCCGATGTGGAACGCCTGACGATCTGAAATCTCTGATAGATAGAGCTCATGAACTTGGTCTGCTTGTACTCATGGATATTGTTCACAGCCATGCATCAAATAATACGTTGGATGGGCTGAACATGTTTGATGGAACCGATAGTCACTACTTCCACTCTGGGTCCCGAGGATATCATTGGATGTGGGATTCTCGCCTTTTCAATTATGGAAGTTGGGAGGTTTTGAGGTATCTTCTCTCAAATGCAAGGTGGTGGTTGGAAGAATTTAAGTTTGACGGGTTCAGATTTGATGGTGTAACTTCAATGATGTACACTCATCATGGATTGCAGGTAGCATTTACCGGAAACTACAGCGAGTATTTTGGATTAGCAACAGATGTAGAGGCTGTCACTTACCTGATGCTGGTTAATGATTTGATTCATGGGCTCTACCCTGAAGCTGTTAGCATTGGTGAAGATGTTAGTGGAATGCCAGCATTTTGTATTCCTGTTGCGGGTGGTGGTGTTGGATTTGATTATCGCTTGCATATGGCTATTGCTGATAAATGGATTGAGCTCCTCCAGAAAATGGATGAAACCTGGCAAATGAGTGATATTGTTCACACACTGACCAACAGAAGATGGGGAGAGAAGTGTGTTGCTTATGCTGAAAGTCATGACCAAGCTCTTGTTGGTGACAAAACAATTGCATTCTGGTTGATGGACAAGGATATGTATGATTTCATGGCTCTGGATAGACCATCAACTCCCACTGTAGATCGTGGAATTGCGTTACACAAAATGATTAGGCTTATTACTATGGGATTAGGTGGAGAAGGATATCTGAATTTTATGGGGAATGAATTTGGACATCCTGAGTGGATTGATTTTCCAAGAGGGGTTCAACATCTTCCAAATGGAAAAATAGTTCCTGGGAACAATAACAGTTTTGACAAATGCCGGCGTAGATTTGATCTTGCAGATGCTGAATATTTAAGATATCATGGCTTGCAAGAATTTGATCGAGCAATGCATCATCTTGAAGAAACCTATAGTTTCATGACTTCTGAGCACCAATATATATCCCGAAAGGATGAAGGAGATAAGGTTATTGTCTTTGAAAGGGGAAACCTGGTCTTTGTCTTCAATTTTCATTGGAGTAAAAGCTATTCAGACTACCGAGTAGGCTGCTTAAAGCCAGGAAAGTATAAGATTGTCTTGGATTCAGATGAGAAAATATTTGGAGGCTTTGATAGGATTGCTCACACCGCGGAGTACTTCACAGTTGATGGATGGTATGATGAGCGGCCTAATTCCTTCCAAGTATATGCACCTTGTAGAACAGCTGTGGTCTATGCTCTAGTAGAGACAAAGGAAACGATACCCATCAAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010021 GO:0010033 GO:0016740 GO:0016757 GO:0016758 GO:0019725 GO:0033500 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0042593 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071326 GO:0071329 GO:0071331 GO:0071332 GO:0071333 GO:0071478 GO:0071482 GO:0071704 GO:0104004 GO:1901135 GO:1901137 GO:1901576 GO:1901700 GO:1901701 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

740

Amino Acids

84.65

Weight (kDa)

5.39

Isoelectric Point (pI)

31.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	97 - 180	1.4e-19	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	246 - 504	1.1e-10	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	635 - 729	4.5e-25	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36390 AT5G03650
fragaria_vesca	FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120
malus_domestica	MD12G1020600.v1.1 MD14G1017700.v1.1
prunus_persica	Prupe.7G115400_v2.0.a1 Prupe.7G115400_v2.0.a1
pyrus_communis	pycom12g01930 pycom14g01610
rosa_chinensis	RchiOBHm_Chr3g0472421
rosa_laevigata	RLG00000024081
rosa_multiflora	Rmu_co8370739.1_g000001 Rmu_sc0001096.1_g000015
rosa_roxburghii	Rroxscaffold_6G00408930
rosa_rugosa	Rorug03G0124700 Rorug03G0124700 Rorug03G0124800 Rorug03G0124900
rosa_samantha	Rh3AG177100 Rh3BG203100 Rh3CG193400 Rh3CG200600 Rh3DG172000
rosa_wichuraiana	Rw3G016440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1165
Acc65I	GGTACC	1 cut(s)	816
AccB1I	GGYRCC	2 cut(s)	337, 816
AccB7I	CCANNNNNTGG	3 cut(s)	140, 581, 1414
AccBSI	CCGCTC	1 cut(s)	2131
AccII	CGCG	1 cut(s)	2096
AciI	CCGC	9 cut(s)	354, 395, 465, 473, 658, 1340, 2094, 2096, 2131
AclWI	GGATC	4 cut(s)	164, 487, 548, 590
AcsI	RAATTY	6 cut(s)	416, 719, 1109, 1672, 1688, 1841
AcuI	CTGAAG	2 cut(s)	495, 1302
AdeI	CACNNNGTG	1 cut(s)	308
AfaI	GTAC	6 cut(s)	115, 555, 818, 915, 1157, 2102
AfiI	CCNNNNNNNGG	9 cut(s)	101, 140, 146, 581, 813, 1249, 1340, 1414, 2216
AflIII	ACRYGT	2 cut(s)	673, 969
AjnI	CCWGG	6 cut(s)	139, 340, 1424, 1756, 1956, 2023
AloI	GAACNNNNNNTCC	2 cut(s)	1737, 1769
Alw21I	GWGCWC	3 cut(s)	895, 1404, 1899
Alw26I	GTCTC	2 cut(s)	637, 2186
AlwI	GGATC	4 cut(s)	164, 487, 548, 590
AlwNI	CAGNNNCTG	1 cut(s)	1811
Ama87I	CYCGRG	2 cut(s)	483, 1011
AoxI	GGCC	1 cut(s)	2132
ApeKI	GCWGC	2 cut(s)	846, 2013
ApoI	RAATTY	6 cut(s)	416, 719, 1109, 1672, 1688, 1841
AseI	ATTAAT	1 cut(s)	362
Asp700I	GAANNNNTTC	1 cut(s)	185
Asp718I	GGTACC	1 cut(s)	816
AspS9I	GGNCC	1 cut(s)	1008
AsuHPI	GGTGA	4 cut(s)	453, 817, 1310, 1532
AvaI	CYCGRG	2 cut(s)	483, 1011
AvaII	GGWCC	1 cut(s)	1008
BaeGI	GKGCMC	1 cut(s)	340
BanI	GGYRCC	2 cut(s)	337, 816
BanII	GRGCYC	3 cut(s)	895, 1275, 1404
BauI	CACGAG	1 cut(s)	276
BbsI	GAAGAC	1 cut(s)	1960
Bbv12I	GWGCWC	3 cut(s)	895, 1404, 1899
BbvCI	CCTCAGC	1 cut(s)	633
BbvI	GCAGC	2 cut(s)	858, 2000
BcgI	CGANNNNNNTGC	4 cut(s)	464, 498, 1995, 2029
BciT130I	CCWGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
BciVI	GTATCC	1 cut(s)	496
BclI	TGATCA	1 cut(s)	193
BcoDI	GTCTC	2 cut(s)	637, 2186
BfaI	CTAG	3 cut(s)	90, 843, 2186
BfmI	CTRYAG	3 cut(s)	1195, 1599, 1876
BfuAI	ACCTGC	1 cut(s)	1165
BfuI	GTATCC	1 cut(s)	496
BisI	GCNGC	3 cut(s)	847, 2014, 2132
BlsI	GCNGC	3 cut(s)	848, 2015, 2133
BmcAI	AGTACT	2 cut(s)	115, 2102
Bme1390I	CCNGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
Bme18I	GGWCC	1 cut(s)	1008
BmeT110I	CYCGRG	2 cut(s)	483, 1011
BmgT120I	GGNCC	1 cut(s)	1008
BmiI	GGNNCC	6 cut(s)	339, 561, 818, 982, 1009, 1010
BmrFI	CCNGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
BmrI	ACTGGG	1 cut(s)	156
BmsI	GCATC	5 cut(s)	365, 950, 1236, 1798, 1867
BmuI	ACTGGG	1 cut(s)	156
BpiI	GAAGAC	1 cut(s)	1960
BpmI	CTGGAG	3 cut(s)	363, 585, 1391
Bpu10I	CCTNAGC	1 cut(s)	633
BsaBI	GATNNNNATC	1 cut(s)	2210
BsaI	GGTCTC	1 cut(s)	637
BsaJI	CCNNGG	4 cut(s)	72, 1012, 1757, 2094
BsaWI	WCCGGW	1 cut(s)	1187
BsaXI	ACNNNNNCTCC	4 cut(s)	639, 669, 1917, 1947
Bsc4I	CCNNNNNNNGG	9 cut(s)	101, 140, 146, 581, 813, 1249, 1340, 1414, 2216
Bse118I	RCCGGY	1 cut(s)	1785
Bse1I	ACTGG	1 cut(s)	151
Bse3DI	GCAATG	1 cut(s)	1860
Bse8I	GATNNNNATC	1 cut(s)	2210
BseBI	CCWGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
BseDI	CCNNGG	4 cut(s)	72, 1012, 1757, 2094
BseGI	GGATG	8 cut(s)	403, 628, 964, 1032, 1423, 1696, 1924, 2123
BseJI	GATNNNNATC	1 cut(s)	2210
BseLI	CCNNNNNNNGG	9 cut(s)	101, 140, 146, 581, 813, 1249, 1340, 1414, 2216
BseMI	GCAATG	1 cut(s)	1860
BseMII	CTCAG	4 cut(s)	422, 647, 1692, 1884
BseNI	ACTGG	1 cut(s)	151
BseRI	GAGGAG	2 cut(s)	623, 1394
BseSI	GKGCMC	1 cut(s)	340
BseXI	GCAGC	2 cut(s)	858, 2000
Bsh1236I	CGCG	1 cut(s)	2096
BshFI	GGCC	1 cut(s)	2134
BshNI	GGYRCC	2 cut(s)	337, 816
BsiHKAI	GWGCWC	3 cut(s)	895, 1404, 1899
BsiHKCI	CYCGRG	2 cut(s)	483, 1011
BsiSI	CCGG	2 cut(s)	1188, 1786
BslFI	GGGAC	2 cut(s)	118, 994
BslI	CCNNNNNNNGG	9 cut(s)	101, 140, 146, 581, 813, 1249, 1340, 1414, 2216
BsmAI	GTCTC	2 cut(s)	637, 2186
BsmFI	GGGAC	2 cut(s)	118, 994
BsmI	GAATGC	4 cut(s)	397, 793, 1320, 1535
BsnI	GGCC	1 cut(s)	2134
Bso31I	GGTCTC	1 cut(s)	637
BsoBI	CYCGRG	2 cut(s)	483, 1011
Bsp1286I	GDGCHC	5 cut(s)	340, 895, 1275, 1404, 1899
Bsp1407I	TGTACA	1 cut(s)	1155
Bsp143I	GATC	9 cut(s)	169, 193, 492, 540, 595, 868, 1603, 1798, 1846
BspACI	CCGC	9 cut(s)	354, 395, 465, 473, 658, 1340, 2094, 2096, 2131
BspANI	GGCC	1 cut(s)	2134
BspCNI	CTCAG	4 cut(s)	421, 646, 1693, 1885
BspFNI	CGCG	1 cut(s)	2096
BspHI	TCATGA	3 cut(s)	895, 1501, 1884
BspLI	GGNNCC	6 cut(s)	339, 561, 818, 982, 1009, 1010
BspMI	ACCTGC	1 cut(s)	1165
BspPI	GGATC	4 cut(s)	164, 487, 548, 590
BspT107I	GGYRCC	2 cut(s)	337, 816
BspTNI	GGTCTC	1 cut(s)	637
BsrBI	CCGCTC	1 cut(s)	2131
BsrDI	GCAATG	1 cut(s)	1860
BsrFI	RCCGGY	1 cut(s)	1785
BsrGI	TGTACA	1 cut(s)	1155
BsrI	ACTGG	1 cut(s)	151
BssAI	RCCGGY	1 cut(s)	1785
BssECI	CCNNGG	4 cut(s)	72, 1012, 1757, 2094
BssMI	GATC	9 cut(s)	169, 193, 492, 540, 595, 868, 1603, 1798, 1846
BssSI	CACGAG	1 cut(s)	276
BssT1I	CCWWGG	1 cut(s)	72
Bst2BI	CACGAG	1 cut(s)	276
Bst2UI	CCWGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
Bst4CI	ACNGT	3 cut(s)	1600, 1772, 2110
Bst6I	CTCTTC	2 cut(s)	600, 635
BstAPI	GCANNNNNTGC	1 cut(s)	116
BstAUI	TGTACA	1 cut(s)	1155
BstC8I	GCNNGC	5 cut(s)	808, 1320, 1370, 1787, 1835
BstDEI	CTNAG	5 cut(s)	348, 408, 633, 1701, 1893
BstDSI	CCRYGG	1 cut(s)	2094
BstENI	CCTNNNNNAGG	1 cut(s)	99
BstF5I	GGATG	8 cut(s)	403, 628, 964, 1032, 1423, 1696, 1924, 2123
BstFNI	CGCG	1 cut(s)	2096
BstKTI	GATC	9 cut(s)	172, 196, 495, 543, 598, 871, 1606, 1801, 1849
BstMAI	GTCTC	2 cut(s)	637, 2186
BstMBI	GATC	9 cut(s)	169, 193, 492, 540, 595, 868, 1603, 1798, 1846
BstMWI	GCNNNNNNNGC	4 cut(s)	116, 344, 353, 2093
BstNI	CCWGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
BstNSI	RCATGY	2 cut(s)	677, 973
BstSCI	CCNGG	6 cut(s)	139, 340, 1424, 1756, 1956, 2023
BstSFI	CTRYAG	3 cut(s)	1195, 1599, 1876
BstSLI	GKGCMC	1 cut(s)	340
BstUI	CGCG	1 cut(s)	2096
BstV1I	GCAGC	2 cut(s)	858, 2000
BstV2I	GAAGAC	1 cut(s)	1960
BstX2I	RGATCY	2 cut(s)	169, 492
BstYI	RGATCY	2 cut(s)	169, 492
BsuI	GTATCC	1 cut(s)	496
BsuRI	GGCC	1 cut(s)	2134
BtgI	CCRYGG	1 cut(s)	2094
BtsCI	GGATG	8 cut(s)	403, 628, 964, 1032, 1423, 1696, 1924, 2123
BtsI	GCAGTG	2 cut(s)	58, 313
BtsIMutI	CAGTG	5 cut(s)	58, 144, 313, 1451, 1596
BveI	ACCTGC	1 cut(s)	1165
Cac8I	GCNNGC	5 cut(s)	808, 1320, 1370, 1787, 1835
CaiI	CAGNNNCTG	1 cut(s)	1811
CciI	TCATGA	3 cut(s)	895, 1501, 1884
Cfr10I	RCCGGY	1 cut(s)	1785
Cfr13I	GGNCC	1 cut(s)	1008
Cfr42I	CCGCGG	1 cut(s)	2097
CsiI	ACCWGGT	1 cut(s)	1956
Csp6I	GTAC	6 cut(s)	114, 554, 817, 914, 1156, 2101
CviQI	GTAC	6 cut(s)	114, 554, 817, 914, 1156, 2101
DdeI	CTNAG	5 cut(s)	348, 408, 633, 1701, 1893
DpnI	GATC	9 cut(s)	171, 195, 494, 542, 597, 870, 1605, 1800, 1848
DpnII	GATC	9 cut(s)	169, 193, 492, 540, 595, 868, 1603, 1798, 1846
DraI	TTTAAA	1 cut(s)	99
DraIII	CACNNNGTG	1 cut(s)	308
Eam1104I	CTCTTC	2 cut(s)	600, 635
EarI	CTCTTC	2 cut(s)	600, 635
Ecl136II	GAGCTC	2 cut(s)	893, 1402
Eco130I	CCWWGG	1 cut(s)	72
Eco24I	GRGCYC	3 cut(s)	895, 1275, 1404
Eco31I	GGTCTC	1 cut(s)	637
Eco32I	GATATC	3 cut(s)	1019, 1667, 1826
Eco47I	GGWCC	1 cut(s)	1008
Eco53kI	GAGCTC	2 cut(s)	893, 1402
Eco57I	CTGAAG	2 cut(s)	495, 1302
Eco88I	CYCGRG	2 cut(s)	483, 1011
EcoICRI	GAGCTC	2 cut(s)	893, 1402
EcoNI	CCTNNNNNAGG	1 cut(s)	99
EcoO109I	RGGNCCY	1 cut(s)	1008
EcoRI	GAATTC	1 cut(s)	719
EcoRII	CCWGG	6 cut(s)	139, 340, 1424, 1756, 1956, 2023
EcoRV	GATATC	3 cut(s)	1019, 1667, 1826
EcoT14I	CCWWGG	1 cut(s)	72
EcoT22I	ATGCAT	2 cut(s)	943, 1860
EcoT38I	GRGCYC	3 cut(s)	895, 1275, 1404
ErhI	CCWWGG	1 cut(s)	72
FalI	AAGNNNNNCTT	2 cut(s)	2027, 2059
FaqI	GGGAC	2 cut(s)	118, 994
FauI	CCCGC	2 cut(s)	472, 1333
FauNDI	CATATG	2 cut(s)	712, 1374
FbaI	TGATCA	1 cut(s)	193
Fnu4HI	GCNGC	3 cut(s)	847, 2014, 2132
FokI	GGATG	8 cut(s)	410, 615, 971, 1039, 1430, 1683, 1931, 2130
FriOI	GRGCYC	3 cut(s)	895, 1275, 1404
Fsp4HI	GCNGC	3 cut(s)	847, 2014, 2132
FspBI	CTAG	3 cut(s)	90, 843, 2186
GluI	GCNGC	3 cut(s)	847, 2014, 2132
GsuI	CTGGAG	3 cut(s)	363, 585, 1391
HaeIII	GGCC	1 cut(s)	2134
HapII	CCGG	2 cut(s)	1188, 1786
HindIII	AAGCTT	1 cut(s)	752
HinfI	GANTC	5 cut(s)	406, 524, 1034, 1264, 2048
HpaII	CCGG	2 cut(s)	1188, 1786
HphI	GGTGA	4 cut(s)	453, 817, 1310, 1532
Hpy166II	GTNNAC	5 cut(s)	300, 461, 931, 1158, 1447
Hpy188I	TCNGA	9 cut(s)	411, 725, 873, 882, 1130, 1671, 1894, 1999, 2053
Hpy8I	GTNNAC	5 cut(s)	300, 461, 931, 1158, 1447
HpyAV	CCTTC	6 cut(s)	175, 248, 519, 1655, 1916, 2152
HpyCH4III	ACNGT	3 cut(s)	1600, 1772, 2110
HpyCH4IV	ACGT	1 cut(s)	953
HpyF10VI	GCNNNNNNNGC	4 cut(s)	116, 344, 353, 2093
HpyF3I	CTNAG	5 cut(s)	348, 408, 633, 1701, 1893
HpySE526I	ACGT	1 cut(s)	953
KflI	GGGWCCC	1 cut(s)	1008
KpnI	GGTACC	1 cut(s)	820
KroI	GCCGGC	1 cut(s)	1785
KroNI	GCCGGC	1 cut(s)	1787
Ksp22I	TGATCA	1 cut(s)	193
KspI	CCGCGG	1 cut(s)	2097
Kzo9I	GATC	9 cut(s)	169, 193, 492, 540, 595, 868, 1603, 1798, 1846
LmnI	GCTCC	5 cut(s)	124, 344, 565, 790, 1407
Lsp1109I	GCAGC	2 cut(s)	858, 2000
LweI	GCATC	5 cut(s)	365, 950, 1236, 1798, 1867
MabI	ACCWGGT	1 cut(s)	1956
MaeI	CTAG	3 cut(s)	90, 843, 2186
MaeII	ACGT	1 cut(s)	953
MaeIII	GTNAC	7 cut(s)	758, 823, 989, 1141, 1234, 1520, 1617
MalI	GATC	9 cut(s)	171, 195, 494, 542, 597, 870, 1605, 1800, 1848
MbiI	CCGCTC	1 cut(s)	2131
MboI	GATC	9 cut(s)	169, 193, 492, 540, 595, 868, 1603, 1798, 1846
MfeI	CAATTG	2 cut(s)	379, 1530
MflI	RGATCY	2 cut(s)	169, 492
MhlI	GDGCHC	5 cut(s)	340, 895, 1275, 1404, 1899
MlyI	GAGTC	2 cut(s)	400, 518
MmeI	TCCRAC	4 cut(s)	658, 936, 1083, 1333
Mph1103I	ATGCAT	2 cut(s)	943, 1860
MroNI	GCCGGC	1 cut(s)	1785
MroXI	GAANNNNTTC	1 cut(s)	185
MseI	TTAA	9 cut(s)	98, 362, 489, 705, 747, 1113, 1254, 1820, 2018
MslI	CAYNNNNRTG	1 cut(s)	936
MspA1I	CMGCKG	2 cut(s)	2096, 2171
MspI	CCGG	2 cut(s)	1188, 1786
MspR9I	CCNGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
MunI	CAATTG	2 cut(s)	379, 1530
Mva1269I	GAATGC	4 cut(s)	397, 793, 1320, 1535
MvaI	CCWGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
MvnI	CGCG	1 cut(s)	2096
MwoI	GCNNNNNNNGC	4 cut(s)	116, 344, 353, 2093
NaeI	GCCGGC	1 cut(s)	1787
NdeI	CATATG	2 cut(s)	712, 1374
NdeII	GATC	9 cut(s)	169, 193, 492, 540, 595, 868, 1603, 1798, 1846
NgoMIV	GCCGGC	1 cut(s)	1785
NlaIV	GGNNCC	6 cut(s)	339, 561, 818, 982, 1009, 1010
NmuCI	GTSAC	4 cut(s)	823, 989, 1234, 1520
NsiI	ATGCAT	2 cut(s)	943, 1860
NspI	RCATGY	2 cut(s)	677, 973
PaeR7I	CTCGAG	1 cut(s)	483
PagI	TCATGA	3 cut(s)	895, 1501, 1884
PciI	ACATGT	2 cut(s)	673, 969
PctI	GAATGC	4 cut(s)	397, 793, 1320, 1535
PdiI	GCCGGC	1 cut(s)	1787
PdmI	GAANNNNTTC	1 cut(s)	185
PfeI	GAWTC	3 cut(s)	1034, 1264, 2048
PflMI	CCANNNNNTGG	3 cut(s)	140, 581, 1414
PkrI	GCNGC	3 cut(s)	848, 2015, 2133
PleI	GAGTC	2 cut(s)	400, 518
PpsI	GAGTC	2 cut(s)	400, 518
PpuMI	RGGWCCY	1 cut(s)	1008
PscI	ACATGT	2 cut(s)	673, 969
PshBI	ATTAAT	1 cut(s)	362
Psp124BI	GAGCTC	2 cut(s)	895, 1404
Psp5II	RGGWCCY	1 cut(s)	1008
Psp6I	CCWGG	6 cut(s)	139, 340, 1424, 1756, 1956, 2023
PspGI	CCWGG	6 cut(s)	139, 340, 1424, 1756, 1956, 2023
PspN4I	GGNNCC	6 cut(s)	339, 561, 818, 982, 1009, 1010
PspPI	GGNCC	1 cut(s)	1008
PspPPI	RGGWCCY	1 cut(s)	1008
PstNI	CAGNNNCTG	1 cut(s)	1811
PsuI	RGATCY	2 cut(s)	169, 492
PvuII	CAGCTG	1 cut(s)	2171
RsaI	GTAC	6 cut(s)	115, 555, 818, 915, 1157, 2102
RsaNI	GTAC	6 cut(s)	114, 554, 817, 914, 1156, 2101
RseI	CAYNNNNRTG	1 cut(s)	936
SacI	GAGCTC	2 cut(s)	895, 1404
SacII	CCGCGG	1 cut(s)	2097
SaqAI	TTAA	9 cut(s)	98, 362, 489, 705, 747, 1113, 1254, 1820, 2018
SatI	GCNGC	3 cut(s)	847, 2014, 2132
Sau3AI	GATC	9 cut(s)	169, 193, 492, 540, 595, 868, 1603, 1798, 1846
Sau96I	GGNCC	1 cut(s)	1008
ScaI	AGTACT	2 cut(s)	115, 2102
SchI	GAGTC	2 cut(s)	400, 518
ScrFI	CCNGG	6 cut(s)	141, 342, 1426, 1758, 1958, 2025
SduI	GDGCHC	5 cut(s)	340, 895, 1275, 1404, 1899
SexAI	ACCWGGT	1 cut(s)	1956
SfaNI	GCATC	5 cut(s)	365, 950, 1236, 1798, 1867
SfcI	CTRYAG	3 cut(s)	1195, 1599, 1876
Sfr274I	CTCGAG	1 cut(s)	483
Sfr303I	CCGCGG	1 cut(s)	2097
SgrBI	CCGCGG	1 cut(s)	2097
SinI	GGWCC	1 cut(s)	1008
SlaI	CTCGAG	1 cut(s)	483
SmiMI	CAYNNNNRTG	1 cut(s)	936
SmlI	CTYRAG	1 cut(s)	483
SmoI	CTYRAG	1 cut(s)	483
SsiI	CCGC	9 cut(s)	354, 395, 465, 473, 658, 1340, 2094, 2096, 2131
SspI	AATATT	2 cut(s)	1817, 2064
SspMI	CTAG	3 cut(s)	90, 843, 2186
SstI	GAGCTC	2 cut(s)	895, 1404
StyD4I	CCNGG	6 cut(s)	139, 340, 1424, 1756, 1956, 2023
StyI	CCWWGG	1 cut(s)	72
TaaI	ACNGT	3 cut(s)	1600, 1772, 2110
TaiI	ACGT	1 cut(s)	956
TaqI	TCGA	2 cut(s)	484, 1849
TatI	WGTACW	4 cut(s)	113, 913, 1155, 2100
TauI	GCSGC	1 cut(s)	2134
TfiI	GAWTC	3 cut(s)	1034, 1264, 2048
Tru1I	TTAA	9 cut(s)	98, 362, 489, 705, 747, 1113, 1254, 1820, 2018
Tru9I	TTAA	9 cut(s)	98, 362, 489, 705, 747, 1113, 1254, 1820, 2018
TscAI	CASTG	5 cut(s)	58, 151, 313, 1458, 1603
TseFI	GTSAC	4 cut(s)	823, 989, 1234, 1520
TseI	GCWGC	2 cut(s)	846, 2013
Tsp45I	GTSAC	4 cut(s)	823, 989, 1234, 1520
TspGWI	ACGGA	2 cut(s)	179, 485
TspRI	CASTG	5 cut(s)	58, 151, 313, 1458, 1603
Van91I	CCANNNNNTGG	3 cut(s)	140, 581, 1414
VpaK11BI	GGWCC	1 cut(s)	1008
VspI	ATTAAT	1 cut(s)	362
XagI	CCTNNNNNAGG	1 cut(s)	99
XapI	RAATTY	6 cut(s)	416, 719, 1109, 1672, 1688, 1841
XceI	RCATGY	2 cut(s)	677, 973
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1514
XhoI	CTCGAG	1 cut(s)	483
XmnI	GAANNNNTTC	1 cut(s)	185
XspI	CTAG	3 cut(s)	90, 843, 2186
ZrmI	AGTACT	2 cut(s)	115, 2102
Zsp2I	ATGCAT	2 cut(s)	943, 1860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.