Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005666

Physical Location & Seq

Genomic Coordinates Reverse (-)

2038235 .. 2052269

Gene Structure 14035 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1020600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGGTCTCGACTCTCTCGGGGATTTGCTTCCCTCTCCTACCTTCCGCCTACACCTCGCATTCCAGCTTCAATGGCGATCGGAGGACCTCCGGCCTCTCTCTCTTCCTCTCCAACACCTCCTCCTCTCGGAAGATCTTTGTTGGAAAGCCTTCGTATGATTCTAATTTGCCATCTTTAGCGGTTGCTGCATCGAAGAAAATGCTTGTTCCTGACAGCCAGAGTGATGGCTCTTCGTCTTTGAAGAAGGAGCAGTCGGGAGCTGCAAGTACAGTTCCAGAGGATCCTCAGGTGATTCAGGACGTAGATAATGTAGCAAAGGAAGATAAAGAGAAGCTTGAGGATGCCCCTTCATTAGTTGTGGCCAATGTTGATGACGCTGAAGCCAAGGTTGAGGACACACCTCGTCCCTTAGAAGTTAAAGCAAGTACTGCTACAAACAAAGCAACAGGAAAGACAATTCCACCGCCTGGCAATGGGCAAAAGATATATGAAATAGATTCACTTTTGGTGGGTCACCGTGATCACCTTGATTACCGTTATGGACAATACAAAAGGCTGCGTGAGGAGATTAACAAGTACGAAGGTGGTTTAGAGGTGTTTTCTCGTGGCTATGAGAAGTTTGGCTTCACACGCAGTGCCAAAGGTATCACTTATAGGGAGTGGGCACCTGGAGCAAAGTCAGCATCACTGATTGGAGATTTCAACAATTGGAACACGAATGCAGATGTGATGACTCAGAATGACTTTGGAGTCTGGGAGATCTTTTTGCCAAACAATGCAGATGGCTCACCTGCAATTCCCCATGGTTCTCGAGTGAAGGTCCGTATGGATACTCCTTCAGGCATCAAAGACTCGATTCCTGCTTGGATCAAGTTTTCCATACAGGCTCCAGGTGAAATCCCATACAATGGCATATACTATGATCCACCAGAAGAGGTATGTTCTTCGGAAAAAAAATCAGTTATGCTTGATACTGGTAGGGATGCCGGGGCTCTGGGTAGAAGAAAGGCTGCAGAAAAGTATGTATTTCAACATTCTCAGCCGAGTAGACCAAAATCACTTCGCATTTATGAGGCACATGTTGGAATGAGTAGCACGGAGCCCAAAATTAACACGTATGCTGAATTCAGAGATGATGTGCTTCCACGCATTAAAAAACTTGGTTACAATGCTGTTCAACTCATGGCTATTCAAGAGCACTCCTACTATGCTAGCTTTGGGTACCATGTCACCAATTTTTTTGCTCCTAGCAGCCGCTGTGGAACCCCTGACGATCTGAAGTCTCTAATAGATAAAGCTCATGAGCTTGGTCTGCTTGTACTCATGGATATTGTTCACAGCCATGCATCAAATAACACATTGGATGGTTTGAACATGTTTGATGGAACCGATAGTCATTACTTCCACTCTGGGTCTCGAGGATATCATTGGATGTGGGATTCCCGCTTGTTTAATTATGGAAGCTGGGAGGTTTTGAGGTATCTTCTTTCCAATGCAAGGTGGTGGCTGGAAGAATACAAGTTCGATGGGTTCAGATTTGATGGTGTGACTTCAATGATGTACACTCACCATGGATTGGAGGTAGCATTCACTGGAAATTACCGTGAGTACTTTGGGTTAGCAACCGATGTAGATGCTGTTACTTACCTGATGCTGGTTAACGATTTGATTCATGGGCTCTACCCTGAAGCTGTTACCATTGGTGAAGATGTTAGTGGAATGCCAACATTCTGTGTTCCTGTTGAAGATGGTGGTGTTGGATTTGATTATCGCCTGCATATGGCTATTGCTGATAAATGGATTGAGCTCCTCCAGAAAATGGATGAAGAGTGGCAAATGGGTGATATTGTTTTCACACTCACCAACAGAAGGTGGGGGGAGAATTGTGTTGCTTATGCCGAAAGTCATGACCAAGCTCTTGTTGGTGACAAAACAATTGCATTTTGGCTGATGGACAAGGATATGTATGATTTCATGGCTCTGGATAGACCATCAACTCCCCTTATAGATCGTGGAATAGCATTACATAAAATGATTAGGCTTATTACTATGGGATTAGGCGGAGAAGGATATCTGAATTTCATGGGAAATGAATTTGGACATCCTGAGTGGATTGATTTTCCAAGAGGCGTTCAACATCTTCCAAATGGAAAAATAGTCCCTGGGAACAATAACAGTTTTGACAAATGCCGACGTAGATTTGATCTTGGGGATGCAGAGTATCTAAGATATCATGGAATGCAAGAATTTGATCGAGCGATGCAACATCTTGAAGAAACCTATGGTTTCATGACTTCTGAGCATCAATACATATCACACAAGGATGAAGGAGACAAAATTATCGTCTTTGAAAGGGGTGACCTGGTTTTTGTCTTCAATTTTCATTGGAGTAACAGCTATTCAGACCACCGAGTTGGCTGCTTAAAGCCAGGAAAGTATAAGATTGTCTTGGATTCAGATGAAAAGTTATTCGGAGGCTTCGATAGGATTGATCACAGTGCTGAGTACTTCACTACTGATGGGTGGTTCGATGAGCGGCCTCATTCTTTCCTACTGTATGCACCTTGTAGAACAGCCGTGGTCTACGCCTTCATAGAGGGACCTAAAAAGGCTGATATCCGACTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010021 GO:0010033 GO:0016740 GO:0016757 GO:0016758 GO:0019725 GO:0033500 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0042593 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071326 GO:0071329 GO:0071331 GO:0071332 GO:0071333 GO:0071478 GO:0071482 GO:0071704 GO:0104004 GO:1901135 GO:1901137 GO:1901576 GO:1901700 GO:1901701 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

876

Amino Acids

98.91

Weight (kDa)

5.54

Isoelectric Point (pI)

32.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	206 - 289	7.4e-20	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	381 - 608	4e-11	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	770 - 863	5.8e-23	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36390 AT5G03650
fragaria_vesca	FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120
malus_domestica	MD12G1020600.v1.1 MD14G1017700.v1.1
prunus_persica	Prupe.7G115400_v2.0.a1 Prupe.7G115400_v2.0.a1
pyrus_communis	pycom12g01930 pycom14g01610
rosa_chinensis	RchiOBHm_Chr3g0472421
rosa_laevigata	RLG00000024081
rosa_multiflora	Rmu_co8370739.1_g000001 Rmu_sc0001096.1_g000015
rosa_roxburghii	Rroxscaffold_6G00408930
rosa_rugosa	Rorug03G0124700 Rorug03G0124700 Rorug03G0124800 Rorug03G0124900
rosa_samantha	Rh3AG177100 Rh3BG203100 Rh3CG193400 Rh3CG200600 Rh3DG172000
rosa_wichuraiana	Rw3G016440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	799
AasI	GACNNNNNNGTC	1 cut(s)	749
Acc36I	ACCTGC	1 cut(s)	799
Acc65I	GGTACC	1 cut(s)	1221
AccB1I	GGYRCC	2 cut(s)	664, 1221
AccB7I	CCANNNNNTGG	4 cut(s)	467, 908, 1576, 1819
AccBSI	CCGCTC	1 cut(s)	2536
AccI	GTMKAC	2 cut(s)	1048, 2583
AciI	CCGC	7 cut(s)	45, 179, 464, 1255, 1444, 2061, 2536
AclWI	GGATC	4 cut(s)	275, 288, 875, 917
AcoI	YGGCCR	1 cut(s)	360
AcsI	RAATTY	4 cut(s)	1124, 2077, 2093, 2246
AcuI	CTGAAG	4 cut(s)	399, 822, 1298, 1707
AdeI	CACNNNGTG	1 cut(s)	635
AfaI	GTAC	8 cut(s)	268, 427, 578, 1223, 1320, 1562, 1610, 2507
AflIII	ACRYGT	3 cut(s)	1078, 1113, 1374
AjnI	CCWGG	6 cut(s)	466, 667, 889, 2161, 2361, 2428
Alw21I	GWGCWC	2 cut(s)	1200, 1809
Alw26I	GTCTC	4 cut(s)	10, 1287, 1419, 2325
AlwI	GGATC	4 cut(s)	275, 288, 875, 917
AlwNI	CAGNNNCTG	1 cut(s)	1257
Ama87I	CYCGRG	3 cut(s)	16, 810, 1416
AoxI	GGCC	3 cut(s)	91, 360, 2537
ApeKI	GCWGC	6 cut(s)	185, 260, 556, 1010, 1251, 2418
ApoI	RAATTY	4 cut(s)	1124, 2077, 2093, 2246
ArsI	GACNNNNNNTTYG	4 cut(s)	2328, 2351, 2360, 2383
Asp700I	GAANNNNTTC	1 cut(s)	148
Asp718I	GGTACC	1 cut(s)	1221
AspS9I	GGNCC	3 cut(s)	84, 820, 2600
AsuC2I	CCSGG	1 cut(s)	988
AsuNHI	GCTAGC	1 cut(s)	1211
AvaI	CYCGRG	3 cut(s)	16, 810, 1416
AvaII	GGWCC	3 cut(s)	84, 820, 2600
AxyI	CCTNAGG	1 cut(s)	285
BaeGI	GKGCMC	1 cut(s)	667
BalI	TGGCCA	1 cut(s)	362
BamHI	GGATCC	1 cut(s)	280
BanI	GGYRCC	2 cut(s)	664, 1221
BanII	GRGCYC	4 cut(s)	994, 1104, 1680, 1809
BauI	CACGAG	1 cut(s)	603
BbsI	GAAGAC	1 cut(s)	2365
Bbv12I	GWGCWC	2 cut(s)	1200, 1809
BbvI	GCAGC	6 cut(s)	172, 247, 543, 997, 1263, 2405
BceAI	ACGGC	1 cut(s)	2561
BcgI	CGANNNNNNTGC	6 cut(s)	181, 215, 1616, 1650, 2400, 2434
BciT130I	CCWGG	6 cut(s)	468, 669, 891, 2163, 2363, 2430
BciVI	GTATCC	1 cut(s)	823
BclI	TGATCA	2 cut(s)	520, 2491
BcnI	CCSGG	1 cut(s)	988
BcoDI	GTCTC	4 cut(s)	10, 1287, 1419, 2325
BfaI	CTAG	2 cut(s)	1212, 1248
BfmI	CTRYAG	1 cut(s)	1011
BfuAI	ACCTGC	1 cut(s)	799
BfuI	GTATCC	1 cut(s)	823
BglII	AGATCT	2 cut(s)	132, 759
BisI	GCNGC	8 cut(s)	186, 261, 557, 1011, 1252, 1255, 2419, 2537
BlsI	GCNGC	8 cut(s)	187, 262, 558, 1012, 1253, 1256, 2420, 2538
BmcAI	AGTACT	3 cut(s)	427, 1610, 2507
Bme1390I	CCNGG	7 cut(s)	468, 669, 891, 988, 2163, 2363, 2430
Bme18I	GGWCC	3 cut(s)	84, 820, 2600
BmeT110I	CYCGRG	3 cut(s)	16, 810, 1416
BmgT120I	GGNCC	3 cut(s)	84, 820, 2600
BmiI	GGNNCC	8 cut(s)	282, 666, 888, 1101, 1223, 1264, 1387, 2601
BmrFI	CCNGG	7 cut(s)	468, 669, 891, 988, 2163, 2363, 2430
BmtI	GCTAGC	1 cut(s)	1215
BpiI	GAAGAC	1 cut(s)	2365
BpmI	CTGGAG	3 cut(s)	690, 873, 1796
BpuEI	CTTGAG	1 cut(s)	356
BpuMI	CCSGG	1 cut(s)	988
BsaAI	YACGTR	1 cut(s)	1116
BsaI	GGTCTC	2 cut(s)	10, 1419
BsaJI	CCNNGG	7 cut(s)	384, 802, 987, 1570, 2161, 2162, 2577
BsaXI	ACNNNNNCTCC	2 cut(s)	104, 134
Bse1I	ACTGG	2 cut(s)	979, 1597
Bse21I	CCTNAGG	1 cut(s)	285
Bse3DI	GCAATG	1 cut(s)	478
BseBI	CCWGG	6 cut(s)	468, 669, 891, 2163, 2363, 2430
BseDI	CCNNGG	7 cut(s)	384, 802, 987, 1570, 2161, 2162, 2577
BseGI	GGATG	8 cut(s)	346, 988, 1369, 1437, 1828, 2101, 2218, 2329
BseMI	GCAATG	1 cut(s)	478
BseMII	CTCAG	6 cut(s)	299, 749, 1052, 2097, 2289, 2493
BseNI	ACTGG	2 cut(s)	979, 1597
BseRI	GAGGAG	4 cut(s)	109, 112, 578, 1799
BseSI	GKGCMC	1 cut(s)	667
BseXI	GCAGC	6 cut(s)	172, 247, 543, 997, 1263, 2405
BseYI	CCCAGC	1 cut(s)	1464
Bsh1285I	CGRYCG	1 cut(s)	79
BshFI	GGCC	3 cut(s)	93, 362, 2539
BshNI	GGYRCC	2 cut(s)	664, 1221
BsiEI	CGRYCG	1 cut(s)	79
BsiHKAI	GWGCWC	2 cut(s)	1200, 1809
BsiHKCI	CYCGRG	3 cut(s)	16, 810, 1416
BsiSI	CCGG	2 cut(s)	90, 987
BslFI	GGGAC	3 cut(s)	390, 2144, 2613
BsmAI	GTCTC	4 cut(s)	10, 1287, 1419, 2325
BsmFI	GGGAC	3 cut(s)	390, 2144, 2613
BsmI	GAATGC	5 cut(s)	58, 724, 1586, 1725, 2244
BsnI	GGCC	3 cut(s)	93, 362, 2539
Bso31I	GGTCTC	2 cut(s)	10, 1419
BsoBI	CYCGRG	3 cut(s)	16, 810, 1416
Bsp1286I	GDGCHC	6 cut(s)	667, 994, 1104, 1200, 1680, 1809
Bsp1407I	TGTACA	1 cut(s)	1560
Bsp19I	CCATGG	2 cut(s)	802, 1570
BspACI	CCGC	7 cut(s)	45, 179, 464, 1255, 1444, 2061, 2536
BspANI	GGCC	3 cut(s)	93, 362, 2539
BspCNI	CTCAG	6 cut(s)	298, 748, 1051, 2098, 2290, 2494
BspHI	TCATGA	3 cut(s)	1300, 1906, 2289
BspLI	GGNNCC	8 cut(s)	282, 666, 888, 1101, 1223, 1264, 1387, 2601
BspMAI	CTGCAG	1 cut(s)	1015
BspMI	ACCTGC	1 cut(s)	799
BspOI	GCTAGC	1 cut(s)	1215
BspPI	GGATC	4 cut(s)	275, 288, 875, 917
BspQI	GCTCTTC	1 cut(s)	235
BspT107I	GGYRCC	2 cut(s)	664, 1221
BspTNI	GGTCTC	2 cut(s)	10, 1419
BsrBI	CCGCTC	1 cut(s)	2536
BsrDI	GCAATG	1 cut(s)	478
BsrGI	TGTACA	1 cut(s)	1560
BsrI	ACTGG	2 cut(s)	979, 1597
BssECI	CCNNGG	7 cut(s)	384, 802, 987, 1570, 2161, 2162, 2577
BssSI	CACGAG	1 cut(s)	603
BssT1I	CCWWGG	3 cut(s)	384, 802, 1570
Bst2BI	CACGAG	1 cut(s)	603
Bst2UI	CCWGG	6 cut(s)	468, 669, 891, 2163, 2363, 2430
Bst4CI	ACNGT	7 cut(s)	271, 518, 536, 1604, 2177, 2498, 2556
Bst6I	CTCTTC	4 cut(s)	107, 235, 927, 1821
BstAPI	GCANNNNNTGC	1 cut(s)	428
BstAUI	TGTACA	1 cut(s)	1560
BstBAI	YACGTR	1 cut(s)	1116
BstC8I	GCNNGC	2 cut(s)	1213, 1775
BstDEI	CTNAG	8 cut(s)	285, 409, 735, 1038, 2106, 2225, 2298, 2502
BstDSI	CCRYGG	3 cut(s)	802, 1570, 2577
BstEII	GGTNACC	2 cut(s)	512, 2357
BstF5I	GGATG	8 cut(s)	346, 988, 1369, 1437, 1828, 2101, 2218, 2329
BstMAI	GTCTC	4 cut(s)	10, 1287, 1419, 2325
BstMCI	CGRYCG	1 cut(s)	79
BstMWI	GCNNNNNNNGC	7 cut(s)	72, 185, 428, 630, 671, 680, 1312
BstNI	CCWGG	6 cut(s)	468, 669, 891, 2163, 2363, 2430
BstNSI	RCATGY	2 cut(s)	1082, 1378
BstPI	GGTNACC	2 cut(s)	512, 2357
BstSCI	CCNGG	7 cut(s)	466, 667, 889, 986, 2161, 2361, 2428
BstSFI	CTRYAG	1 cut(s)	1011
BstSLI	GKGCMC	1 cut(s)	667
BstV1I	GCAGC	6 cut(s)	172, 247, 543, 997, 1263, 2405
BstV2I	GAAGAC	1 cut(s)	2365
BstX2I	RGATCY	3 cut(s)	132, 280, 759
BstXI	CCANNNNNNTGG	2 cut(s)	224, 2414
BstYI	RGATCY	3 cut(s)	132, 280, 759
Bsu36I	CCTNAGG	1 cut(s)	285
BsuI	GTATCC	1 cut(s)	823
BsuRI	GGCC	3 cut(s)	93, 362, 2539
BtgI	CCRYGG	3 cut(s)	802, 1570, 2577
BtgZI	GCGATG	1 cut(s)	2273
BtsCI	GGATG	8 cut(s)	346, 988, 1369, 1437, 1828, 2101, 2218, 2329
BtsI	GCAGTG	1 cut(s)	640
BtsIMutI	CAGTG	4 cut(s)	640, 686, 1590, 2503
BveI	ACCTGC	1 cut(s)	799
Cac8I	GCNNGC	2 cut(s)	1213, 1775
CaiI	CAGNNNCTG	1 cut(s)	1257
CciI	TCATGA	3 cut(s)	1300, 1906, 2289
Cfr13I	GGNCC	3 cut(s)	84, 820, 2600
CseI	GACGC	1 cut(s)	383
CsiI	ACCWGGT	1 cut(s)	2361
Csp6I	GTAC	8 cut(s)	267, 426, 577, 1222, 1319, 1561, 1609, 2506
CviQI	GTAC	8 cut(s)	267, 426, 577, 1222, 1319, 1561, 1609, 2506
DdeI	CTNAG	8 cut(s)	285, 409, 735, 1038, 2106, 2225, 2298, 2502
DraIII	CACNNNGTG	1 cut(s)	635
DrdI	GACNNNNNNGTC	1 cut(s)	749
DseDI	GACNNNNNNGTC	1 cut(s)	749
EaeI	YGGCCR	1 cut(s)	360
Eam1104I	CTCTTC	4 cut(s)	107, 235, 927, 1821
EarI	CTCTTC	4 cut(s)	107, 235, 927, 1821
EciI	GGCGGA	2 cut(s)	34, 2076
Ecl136II	GAGCTC	1 cut(s)	1807
Eco130I	CCWWGG	3 cut(s)	384, 802, 1570
Eco24I	GRGCYC	4 cut(s)	994, 1104, 1680, 1809
Eco31I	GGTCTC	2 cut(s)	10, 1419
Eco32I	GATATC	4 cut(s)	1424, 2072, 2231, 2617
Eco47I	GGWCC	3 cut(s)	84, 820, 2600
Eco53kI	GAGCTC	1 cut(s)	1807
Eco57I	CTGAAG	4 cut(s)	399, 822, 1298, 1707
Eco81I	CCTNAGG	1 cut(s)	285
Eco88I	CYCGRG	3 cut(s)	16, 810, 1416
Eco91I	GGTNACC	2 cut(s)	512, 2357
EcoICRI	GAGCTC	1 cut(s)	1807
EcoO109I	RGGNCCY	2 cut(s)	84, 2600
EcoO65I	GGTNACC	2 cut(s)	512, 2357
EcoRI	GAATTC	1 cut(s)	1124
EcoRII	CCWGG	6 cut(s)	466, 667, 889, 2161, 2361, 2428
EcoRV	GATATC	4 cut(s)	1424, 2072, 2231, 2617
EcoT14I	CCWWGG	3 cut(s)	384, 802, 1570
EcoT22I	ATGCAT	1 cut(s)	1348
EcoT38I	GRGCYC	4 cut(s)	994, 1104, 1680, 1809
ErhI	CCWWGG	3 cut(s)	384, 802, 1570
FalI	AAGNNNNNCTT	6 cut(s)	318, 350, 608, 640, 2432, 2464
FaqI	GGGAC	3 cut(s)	390, 2144, 2613
FauI	CCCGC	1 cut(s)	1451
FauNDI	CATATG	1 cut(s)	1779
FbaI	TGATCA	2 cut(s)	520, 2491
FblI	GTMKAC	2 cut(s)	1048, 2583
Fnu4HI	GCNGC	8 cut(s)	186, 261, 557, 1011, 1252, 1255, 2419, 2537
FokI	GGATG	8 cut(s)	353, 995, 1376, 1444, 1835, 2088, 2225, 2336
FriOI	GRGCYC	4 cut(s)	994, 1104, 1680, 1809
Fsp4HI	GCNGC	8 cut(s)	186, 261, 557, 1011, 1252, 1255, 2419, 2537
FspBI	CTAG	2 cut(s)	1212, 1248
GluI	GCNGC	8 cut(s)	186, 261, 557, 1011, 1252, 1255, 2419, 2537
GsaI	CCCAGC	1 cut(s)	1468
GsuI	CTGGAG	3 cut(s)	690, 873, 1796
HaeIII	GGCC	3 cut(s)	93, 362, 2539
HapII	CCGG	2 cut(s)	90, 987
HgaI	GACGC	1 cut(s)	383
HincII	GTYRAC	1 cut(s)	1660
HindII	GTYRAC	1 cut(s)	1660
HindIII	AAGCTT	1 cut(s)	332
HpaI	GTTAAC	1 cut(s)	1660
HpaII	CCGG	2 cut(s)	90, 987
Hpy166II	GTNNAC	5 cut(s)	1049, 1336, 1563, 1660, 2584
Hpy8I	GTNNAC	5 cut(s)	1049, 1336, 1563, 1660, 2584
Hpy99I	CGWCG	1 cut(s)	2196
HpyCH4III	ACNGT	7 cut(s)	271, 518, 536, 1604, 2177, 2498, 2556
HpyCH4IV	ACGT	3 cut(s)	300, 1115, 2194
HpyF10VI	GCNNNNNNNGC	7 cut(s)	72, 185, 428, 630, 671, 680, 1312
HpyF3I	CTNAG	8 cut(s)	285, 409, 735, 1038, 2106, 2225, 2298, 2502
HpySE526I	ACGT	3 cut(s)	300, 1115, 2194
KpnI	GGTACC	1 cut(s)	1225
Ksp22I	TGATCA	2 cut(s)	520, 2491
KspAI	GTTAAC	1 cut(s)	1660
LguI	GCTCTTC	1 cut(s)	235
LmnI	GCTCC	7 cut(s)	247, 257, 671, 892, 1099, 1249, 1812
Lsp1109I	GCAGC	6 cut(s)	172, 247, 543, 997, 1263, 2405
MabI	ACCWGGT	1 cut(s)	2361
MaeI	CTAG	2 cut(s)	1212, 1248
MaeII	ACGT	3 cut(s)	300, 1115, 2194
MaeIII	GTNAC	9 cut(s)	512, 1163, 1228, 1546, 1639, 1693, 1925, 2357, 2390
MbiI	CCGCTC	1 cut(s)	2536
MfeI	CAATTG	2 cut(s)	706, 1935
MflI	RGATCY	3 cut(s)	132, 280, 759
MhlI	GDGCHC	6 cut(s)	667, 994, 1104, 1200, 1680, 1809
MlsI	TGGCCA	1 cut(s)	362
MluNI	TGGCCA	1 cut(s)	362
MlyI	GAGTC	5 cut(s)	4, 727, 759, 845, 2616
MmeI	TCCRAC	4 cut(s)	121, 135, 1063, 1738
Mox20I	TGGCCA	1 cut(s)	362
Mph1103I	ATGCAT	1 cut(s)	1348
MroXI	GAANNNNTTC	1 cut(s)	148
MscI	TGGCCA	1 cut(s)	362
MseI	TTAA	7 cut(s)	417, 570, 1110, 1152, 1452, 1659, 2423
MslI	CAYNNNNRTG	2 cut(s)	1341, 2322
Msp20I	TGGCCA	1 cut(s)	362
MspA1I	CMGCKG	1 cut(s)	1257
MspI	CCGG	2 cut(s)	90, 987
MspR9I	CCNGG	7 cut(s)	468, 669, 891, 988, 2163, 2363, 2430
MunI	CAATTG	2 cut(s)	706, 1935
Mva1269I	GAATGC	5 cut(s)	58, 724, 1586, 1725, 2244
MvaI	CCWGG	6 cut(s)	468, 669, 891, 2163, 2363, 2430
MwoI	GCNNNNNNNGC	7 cut(s)	72, 185, 428, 630, 671, 680, 1312
NciI	CCSGG	1 cut(s)	988
NcoI	CCATGG	2 cut(s)	802, 1570
NdeI	CATATG	1 cut(s)	1779
NheI	GCTAGC	1 cut(s)	1211
NlaIV	GGNNCC	8 cut(s)	282, 666, 888, 1101, 1223, 1264, 1387, 2601
NmeAIII	GCCGAG	1 cut(s)	1068
NmuCI	GTSAC	5 cut(s)	512, 1228, 1546, 1925, 2357
NsiI	ATGCAT	1 cut(s)	1348
NspI	RCATGY	2 cut(s)	1082, 1378
PaeR7I	CTCGAG	2 cut(s)	810, 1416
PagI	TCATGA	3 cut(s)	1300, 1906, 2289
PaqCI	CACCTGC	1 cut(s)	799
PasI	CCCWGGG	1 cut(s)	2162
PciI	ACATGT	2 cut(s)	1078, 1374
PciSI	GCTCTTC	1 cut(s)	235
PcsI	WCGNNNNNNNCGW	1 cut(s)	2478
PctI	GAATGC	5 cut(s)	58, 724, 1586, 1725, 2244
PdmI	GAANNNNTTC	1 cut(s)	148
PfeI	GAWTC	7 cut(s)	158, 292, 497, 856, 1439, 1669, 2453
PflMI	CCANNNNNTGG	4 cut(s)	467, 908, 1576, 1819
PkrI	GCNGC	8 cut(s)	187, 262, 558, 1012, 1253, 1256, 2420, 2538
Ple19I	CGATCG	1 cut(s)	79
PleI	GAGTC	5 cut(s)	4, 727, 758, 845, 2616
PpsI	GAGTC	5 cut(s)	4, 727, 758, 845, 2616
Ppu21I	YACGTR	1 cut(s)	1116
PpuMI	RGGWCCY	2 cut(s)	84, 2600
PscI	ACATGT	2 cut(s)	1078, 1374
Psp124BI	GAGCTC	1 cut(s)	1809
Psp5II	RGGWCCY	2 cut(s)	84, 2600
Psp6I	CCWGG	6 cut(s)	466, 667, 889, 2161, 2361, 2428
PspEI	GGTNACC	2 cut(s)	512, 2357
PspFI	CCCAGC	1 cut(s)	1464
PspGI	CCWGG	6 cut(s)	466, 667, 889, 2161, 2361, 2428
PspN4I	GGNNCC	8 cut(s)	282, 666, 888, 1101, 1223, 1264, 1387, 2601
PspPI	GGNCC	3 cut(s)	84, 820, 2600
PspPPI	RGGWCCY	2 cut(s)	84, 2600
PstI	CTGCAG	1 cut(s)	1015
PstNI	CAGNNNCTG	1 cut(s)	1257
PsuI	RGATCY	3 cut(s)	132, 280, 759
PvuI	CGATCG	1 cut(s)	79
RsaI	GTAC	8 cut(s)	268, 427, 578, 1223, 1320, 1562, 1610, 2507
RsaNI	GTAC	8 cut(s)	267, 426, 577, 1222, 1319, 1561, 1609, 2506
RseI	CAYNNNNRTG	2 cut(s)	1341, 2322
SacI	GAGCTC	1 cut(s)	1809
SapI	GCTCTTC	1 cut(s)	235
SaqAI	TTAA	7 cut(s)	417, 570, 1110, 1152, 1452, 1659, 2423
SatI	GCNGC	8 cut(s)	186, 261, 557, 1011, 1252, 1255, 2419, 2537
Sau96I	GGNCC	3 cut(s)	84, 820, 2600
ScaI	AGTACT	3 cut(s)	427, 1610, 2507
SchI	GAGTC	5 cut(s)	4, 727, 759, 845, 2616
ScrFI	CCNGG	7 cut(s)	468, 669, 891, 988, 2163, 2363, 2430
SduI	GDGCHC	6 cut(s)	667, 994, 1104, 1200, 1680, 1809
SexAI	ACCWGGT	1 cut(s)	2361
SfcI	CTRYAG	1 cut(s)	1011
Sfr274I	CTCGAG	2 cut(s)	810, 1416
SinI	GGWCC	3 cut(s)	84, 820, 2600
SlaI	CTCGAG	2 cut(s)	810, 1416
SmiMI	CAYNNNNRTG	2 cut(s)	1341, 2322
SmlI	CTYRAG	3 cut(s)	335, 810, 1416
SmoI	CTYRAG	3 cut(s)	335, 810, 1416
SsiI	CCGC	7 cut(s)	45, 179, 464, 1255, 1444, 2061, 2536
SspMI	CTAG	2 cut(s)	1212, 1248
SstI	GAGCTC	1 cut(s)	1809
StyD4I	CCNGG	7 cut(s)	466, 667, 889, 986, 2161, 2361, 2428
StyI	CCWWGG	3 cut(s)	384, 802, 1570
TaaI	ACNGT	7 cut(s)	271, 518, 536, 1604, 2177, 2498, 2556
TaiI	ACGT	3 cut(s)	303, 1118, 2197
TaqI	TCGA	9 cut(s)	8, 191, 811, 854, 1417, 1524, 2254, 2481, 2529
TatI	WGTACW	6 cut(s)	266, 425, 1318, 1560, 1608, 2505
TauI	GCSGC	2 cut(s)	1257, 2539
TfiI	GAWTC	7 cut(s)	158, 292, 497, 856, 1439, 1669, 2453
Tru1I	TTAA	7 cut(s)	417, 570, 1110, 1152, 1452, 1659, 2423
Tru9I	TTAA	7 cut(s)	417, 570, 1110, 1152, 1452, 1659, 2423
TscAI	CASTG	4 cut(s)	640, 693, 1597, 2503
TseFI	GTSAC	5 cut(s)	512, 1228, 1546, 1925, 2357
TseI	GCWGC	6 cut(s)	185, 260, 556, 1010, 1251, 2418
Tsp45I	GTSAC	5 cut(s)	512, 1228, 1546, 1925, 2357
TspGWI	ACGGA	2 cut(s)	812, 1112
TspRI	CASTG	4 cut(s)	640, 693, 1597, 2503
Van91I	CCANNNNNTGG	4 cut(s)	467, 908, 1576, 1819
VpaK11BI	GGWCC	3 cut(s)	84, 820, 2600
XapI	RAATTY	4 cut(s)	1124, 2077, 2093, 2246
XceI	RCATGY	2 cut(s)	1082, 1378
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1919
XhoI	CTCGAG	2 cut(s)	810, 1416
XmiI	GTMKAC	2 cut(s)	1048, 2583
XmnI	GAANNNNTTC	1 cut(s)	148
XspI	CTAG	2 cut(s)	1212, 1248
ZrmI	AGTACT	3 cut(s)	427, 1610, 2507
Zsp2I	ATGCAT	1 cut(s)	1348

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.