Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0005666

Physical Location & Seq

Genomic Coordinates Reverse (-)

11943239 .. 11954894

Gene Structure 11656 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g18120.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGTTGATTACCTCTGTTTCTTCTTTTGGGTTTATGTGTTTCAGATGGGACACAATGGCCTTTATGTTGGGGGTTATTCCGAACATGTGCTTAATTCGCGGGAAGATCTTTGCCGGAAAGTCTTCATATGATTCTGATTCAGCTTCTTCTTTAACTGTTGCTGCTTCAAAGAAAATTCTTGTTCCTGGAGGTCCGAGTGATGGCTCTCCACCTTTGACAGAGGAATTGGAAGCTCCCGACACTGATTCAGAGGATCCTCAGGTGCTAGAGGATGTAGACAGTGTAACTATGGAAGATGAGAAGATTGAAGATGAAGCAATTTCTTCGTTAGATGTGGGCAGTGTTGATGATGAAACACCTAGTCCCTTAAAAGCAACAGCAAGTACTGCTACAAAGACAATTCCACCACCTGGCACTGGGCAAAAAATATATGAAATAGATCCACTTTTGAAGGGCTTCCGGGATCATCTCGATTACCGTTATGGACAATACAAAAGGCTGCGTGAGGAAATTGACAAGTATGAAGGTGGTTTGGAGGTGTTTTCTCGTGGCTATGAGAAGTTTGGGTTCACACGCAGTGCCACAGGTATCACTTATAGGGAGTGGGCACCTGGAGCTAAGTCCGCATCATTAATTGGAGATTTCAACAATTGGAATATGAATGCGGATGTGATGACTCGGAATGAATTTGGGGTCTGGGAGATCTTTTTGCCAAACAATGCAGATGGTTCACCCGCAATTCCACATGGTTCTCGAGTGAAGATCCGTATGGATACCCCTTCAGGTATCAAAGACTCAATTCCTGCTTGGATCAAGTTTTCGGTACAGGCTCCTAATGAAATCCCATACAATGGAATATACTATGATCCTCCAGAAGAGGAGAAGTATGTATTTCAACATCCTCAGCCAAATAGACCACAATCACTCCGCATTTACGAGGCACATGTTGGAATGAGTAGCACAGAGCCAAAAATTAACTCGTATGCTGAATTCAGAGATGATGTGCTACCACGCATTAAAAAGCTTGGGTACAATGCTGTTCAGCTCATGGCTATTCAGGAGCATGCCTACTATGCAAGCTTTGGGTACCATGTCACCAATTTTTTTGCGCCTAGCAGCCGATGTGGAACACCTGACGATCTGAAATCTCTGATAGATAGGGCTCATGAACTTGGTCTGCTTGTACTCATGGATATTGTTCACAGCCATGCATCAAATAATACGTTGGATGGGCTGAACATGTTTGATGGAACCGATAGTCACTACTTCCACTCTGGGCCCCGTGGATATCATTGGATGTGGGATTCTCGCCTTTTCAATTATGGAAGTTGGGAGGTTTTGAGGTATCTTCTCTCAAATGCAAGGTGGTGGTTGGAAGAATTTAAGTTTGACGGGTTCAGATTTGATGGTGTAACTTCAATGATGTATACTCACCATGGATTGCAGGTAGCATTTACTGGAAACTACAGCGAGTATTTTGGATTAGCAACAGATGTAGATGCTGTTACTTACCTGATGCTGGTTAATGATTTGATTCACGGGCTCTACCCCGAAGCTATTAGCATCGGTGAAGATGTTAGTGGAATGCCAGCATTTTGTATTCCTGTTGCAGATGGCGGTGTTGGATTTGATTATCGCCTGCATATGGCTATTGCTGATAAATGGATTGAGCTCCTCCAAAAAATGGATGAGTACTGGCAAATGGGTGATATTGTTCACACGCTGACCAACAGAAGATGGGGAGAGAAGTGTGTTGCTTATGCGGAAAGTCATGACCAAGCTCTTGTTGGTGACAAAACAATTGCATTCTGGTTGATGGACAAGGATATGTATGATTTCATGGCTCTGGATAGACCATCAACTCCCACTATAGACCGTGGAATTGCATTACACAAAATGATTAGGCTTATTACTATGGGATTAGGTGGAGAAGGATATCTGAATTTTATGGGGAATGAATTTGGACATCCTGAGTGGATTGATTTTCCAAGAGGTGTTCAAAAACTTCCAAATGGAAAAATAGTTCCTGGGAACAACAACAGTTTTGACAAATGCCGGCGTAGATTTGATCTTGCAGATGCTGACTATCTGAGATATCATGGCTTGCAAGAATTTGATCGAGCAATGCATCATCTTGAAGAAACTTATAGTTTCATGACTTCTGAGCACCAATATATATCTCGAAAGGATGAAGGAGATAAAGTTATCGTCTTTGAAAGGGGAAACCTGGTTTTTGTGTTCAATTTTCATTGGAGTAAAAGCTATTCAGACTACCGAGTAGGCTGCTTAAAGCCAGGAAAGTATAAGATTGTCTTGGATTCAGATGAGTCAATATTTGGAGGCTTCAATAGGATTGATCACACCGCTGATTACTTCACTGTTGACGGATGGTATGATGAGCGGCCCAATTCCTTCCAACTATATGCACCTTGTAGAACAGCTGTGGTGTATGCTCTAGTAGAGAGCAAGGAAACGAGCAAGGAAAAGAAACCCATCAAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010021 GO:0010033 GO:0016740 GO:0016757 GO:0016758 GO:0019725 GO:0033500 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0042593 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071326 GO:0071329 GO:0071331 GO:0071332 GO:0071333 GO:0071478 GO:0071482 GO:0071704 GO:0104004 GO:1901135 GO:1901137 GO:1901576 GO:1901700 GO:1901701 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

835

Amino Acids

94.79

Weight (kDa)

5.16

Isoelectric Point (pI)

33.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	198 - 281	1.1e-19	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	347 - 562	3.5e-10	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	736 - 830	3.3e-25	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36390 AT5G03650
fragaria_vesca	FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120
malus_domestica	MD12G1020600.v1.1 MD14G1017700.v1.1
prunus_persica	Prupe.7G115400_v2.0.a1 Prupe.7G115400_v2.0.a1
pyrus_communis	pycom12g01930 pycom14g01610
rosa_chinensis	RchiOBHm_Chr3g0472421
rosa_laevigata	RLG00000024081
rosa_multiflora	Rmu_co8370739.1_g000001 Rmu_sc0001096.1_g000015
rosa_roxburghii	Rroxscaffold_6G00408930
rosa_rugosa	Rorug03G0124700 Rorug03G0124700 Rorug03G0124800 Rorug03G0124900
rosa_samantha	Rh3AG177100 Rh3BG203100 Rh3CG193400 Rh3CG200600 Rh3DG172000
rosa_wichuraiana	Rw3G016440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1435
Acc65I	GGTACC	1 cut(s)	1086
AccB1I	GGYRCC	2 cut(s)	607, 1086
AccB7I	CCANNNNNTGG	3 cut(s)	410, 851, 1684
AccBSI	CCGCTC	1 cut(s)	2401
AccI	GTMKAC	2 cut(s)	276, 1427
AccII	CGCG	1 cut(s)	99
AciI	CCGC	9 cut(s)	99, 624, 665, 735, 928, 1617, 1763, 2364, 2401
AclWI	GGATC	7 cut(s)	248, 261, 434, 471, 757, 818, 860
AcsI	RAATTY	7 cut(s)	174, 686, 989, 1379, 1942, 1958, 2111
AcuI	CTGAAG	1 cut(s)	765
AdeI	CACNNNGTG	1 cut(s)	578
AfaI	GTAC	6 cut(s)	385, 825, 1031, 1088, 1185, 1694
AfiI	CCNNNNNNNGG	8 cut(s)	200, 410, 416, 851, 1519, 1645, 1684, 2498
AflIII	ACRYGT	3 cut(s)	84, 943, 1239
AjnI	CCWGG	6 cut(s)	184, 409, 610, 2026, 2226, 2293
AloI	GAACNNNNNNTCC	2 cut(s)	2007, 2039
Alw21I	GWGCWC	2 cut(s)	1674, 2169
AlwI	GGATC	7 cut(s)	248, 261, 434, 471, 757, 818, 860
AlwNI	CAGNNNCTG	1 cut(s)	2081
Ama87I	CYCGRG	1 cut(s)	753
AoxI	GGCC	3 cut(s)	57, 1277, 2402
ApaI	GGGCCC	1 cut(s)	1281
ApeKI	GCWGC	4 cut(s)	161, 499, 1116, 2283
ApoI	RAATTY	7 cut(s)	174, 686, 989, 1379, 1942, 1958, 2111
AseI	ATTAAT	1 cut(s)	632
Asp700I	GAANNNNTTC	2 cut(s)	121, 455
Asp718I	GGTACC	1 cut(s)	1086
AspLEI	GCGC	1 cut(s)	1111
AspS9I	GGNCC	4 cut(s)	191, 1277, 1278, 2403
AsuC2I	CCSGG	1 cut(s)	461
AsuHPI	GGTGA	6 cut(s)	723, 1087, 1424, 1580, 1718, 1802
AvaI	CYCGRG	1 cut(s)	753
AvaII	GGWCC	1 cut(s)	191
AxyI	CCTNAGG	1 cut(s)	258
BaeGI	GKGCMC	2 cut(s)	610, 1281
BamHI	GGATCC	1 cut(s)	253
BanI	GGYRCC	2 cut(s)	607, 1086
BanII	GRGCYC	4 cut(s)	1165, 1281, 1545, 1674
BauI	CACGAG	1 cut(s)	546
BbsI	GAAGAC	1 cut(s)	114
Bbv12I	GWGCWC	2 cut(s)	1674, 2169
BbvCI	CCTCAGC	1 cut(s)	903
BbvI	GCAGC	4 cut(s)	148, 486, 1128, 2270
BcgI	CGANNNNNNTGC	4 cut(s)	306, 340, 2265, 2299
BciT130I	CCWGG	6 cut(s)	186, 411, 612, 2028, 2228, 2295
BciVI	GTATCC	1 cut(s)	766
BclI	TGATCA	1 cut(s)	2356
BcnI	CCSGG	1 cut(s)	461
BfaI	CTAG	4 cut(s)	266, 360, 1113, 2456
BfmI	CTRYAG	2 cut(s)	1465, 1869
BfuAI	ACCTGC	1 cut(s)	1435
BfuI	GTATCC	1 cut(s)	766
BglII	AGATCT	2 cut(s)	105, 702
BisI	GCNGC	5 cut(s)	162, 500, 1117, 2284, 2402
BlsI	GCNGC	5 cut(s)	163, 501, 1118, 2285, 2403
BmcAI	AGTACT	2 cut(s)	385, 1694
Bme1390I	CCNGG	7 cut(s)	186, 411, 461, 612, 2028, 2228, 2295
Bme18I	GGWCC	1 cut(s)	191
BmeT110I	CYCGRG	1 cut(s)	753
BmgT120I	GGNCC	4 cut(s)	191, 1277, 1278, 2403
BmiI	GGNNCC	7 cut(s)	255, 609, 831, 1088, 1252, 1279, 1280
BmrFI	CCNGG	7 cut(s)	186, 411, 461, 612, 2028, 2228, 2295
BmrI	ACTGGG	1 cut(s)	426
BmsI	GCATC	7 cut(s)	635, 1220, 1489, 1506, 1572, 2068, 2137
BmuI	ACTGGG	1 cut(s)	426
BpiI	GAAGAC	1 cut(s)	114
BpmI	CTGGAG	3 cut(s)	207, 633, 855
Bpu10I	CCTNAGC	1 cut(s)	903
BpuMI	CCSGG	1 cut(s)	461
BsaJI	CCNNGG	4 cut(s)	1282, 1435, 1876, 2027
BsaXI	ACNNNNNCTCC	4 cut(s)	909, 939, 2187, 2217
Bsc4I	CCNNNNNNNGG	8 cut(s)	200, 410, 416, 851, 1519, 1645, 1684, 2498
Bse118I	RCCGGY	1 cut(s)	2055
Bse1I	ACTGG	3 cut(s)	421, 1462, 1700
Bse21I	CCTNAGG	1 cut(s)	258
Bse3DI	GCAATG	1 cut(s)	2130
BseBI	CCWGG	6 cut(s)	186, 411, 612, 2028, 2228, 2295
BseDI	CCNNGG	4 cut(s)	1282, 1435, 1876, 2027
BseGI	GGATG	9 cut(s)	277, 673, 898, 1234, 1302, 1693, 1966, 2194, 2393
BseLI	CCNNNNNNNGG	8 cut(s)	200, 410, 416, 851, 1519, 1645, 1684, 2498
BseMI	GCAATG	1 cut(s)	2130
BseMII	CTCAG	5 cut(s)	272, 917, 1962, 2081, 2154
BseNI	ACTGG	3 cut(s)	421, 1462, 1700
BseRI	GAGGAG	2 cut(s)	893, 1664
BseSI	GKGCMC	2 cut(s)	610, 1281
BseXI	GCAGC	4 cut(s)	148, 486, 1128, 2270
Bsh1236I	CGCG	1 cut(s)	99
BshFI	GGCC	3 cut(s)	59, 1279, 2404
BshNI	GGYRCC	2 cut(s)	607, 1086
BsiHKAI	GWGCWC	2 cut(s)	1674, 2169
BsiHKCI	CYCGRG	1 cut(s)	753
BsiSI	CCGG	3 cut(s)	114, 460, 2056
BslFI	GGGAC	2 cut(s)	62, 348
BslI	CCNNNNNNNGG	8 cut(s)	200, 410, 416, 851, 1519, 1645, 1684, 2498
BsmFI	GGGAC	2 cut(s)	62, 348
BsmI	GAATGC	3 cut(s)	667, 1590, 1805
BsnI	GGCC	3 cut(s)	59, 1279, 2404
BsoBI	CYCGRG	1 cut(s)	753
Bsp120I	GGGCCC	1 cut(s)	1277
Bsp1286I	GDGCHC	6 cut(s)	610, 1165, 1281, 1545, 1674, 2169
Bsp19I	CCATGG	1 cut(s)	1435
BspACI	CCGC	9 cut(s)	99, 624, 665, 735, 928, 1617, 1763, 2364, 2401
BspANI	GGCC	3 cut(s)	59, 1279, 2404
BspCNI	CTCAG	5 cut(s)	271, 916, 1963, 2082, 2155
BspFNI	CGCG	1 cut(s)	99
BspHI	TCATGA	3 cut(s)	1165, 1771, 2154
BspLI	GGNNCC	7 cut(s)	255, 609, 831, 1088, 1252, 1279, 1280
BspMI	ACCTGC	1 cut(s)	1435
BspPI	GGATC	7 cut(s)	248, 261, 434, 471, 757, 818, 860
BspT107I	GGYRCC	2 cut(s)	607, 1086
BsrBI	CCGCTC	1 cut(s)	2401
BsrDI	GCAATG	1 cut(s)	2130
BsrFI	RCCGGY	1 cut(s)	2055
BsrI	ACTGG	3 cut(s)	421, 1462, 1700
BssAI	RCCGGY	1 cut(s)	2055
BssECI	CCNNGG	4 cut(s)	1282, 1435, 1876, 2027
BssNAI	GTATAC	1 cut(s)	1428
BssSI	CACGAG	1 cut(s)	546
BssT1I	CCWWGG	1 cut(s)	1435
Bst1107I	GTATAC	1 cut(s)	1428
Bst2BI	CACGAG	1 cut(s)	546
Bst2UI	CCWGG	6 cut(s)	186, 411, 612, 2028, 2228, 2295
Bst4CI	ACNGT	6 cut(s)	157, 281, 479, 1877, 2042, 2380
Bst6I	CTCTTC	1 cut(s)	870
BstAPI	GCANNNNNTGC	1 cut(s)	386
BstC8I	GCNNGC	6 cut(s)	1065, 1078, 1590, 1640, 2057, 2105
BstDEI	CTNAG	6 cut(s)	258, 618, 903, 1971, 2090, 2163
BstDSI	CCRYGG	3 cut(s)	1282, 1435, 1876
BstF5I	GGATG	9 cut(s)	277, 673, 898, 1234, 1302, 1693, 1966, 2194, 2393
BstFNI	CGCG	1 cut(s)	99
BstHHI	GCGC	1 cut(s)	1111
BstMWI	GCNNNNNNNGC	5 cut(s)	96, 386, 614, 623, 1073
BstNI	CCWGG	6 cut(s)	186, 411, 612, 2028, 2228, 2295
BstNSI	RCATGY	4 cut(s)	88, 947, 1067, 1243
BstSCI	CCNGG	7 cut(s)	184, 409, 459, 610, 2026, 2226, 2293
BstSFI	CTRYAG	2 cut(s)	1465, 1869
BstSLI	GKGCMC	2 cut(s)	610, 1281
BstUI	CGCG	1 cut(s)	99
BstV1I	GCAGC	4 cut(s)	148, 486, 1128, 2270
BstV2I	GAAGAC	1 cut(s)	114
BstX2I	RGATCY	5 cut(s)	105, 253, 439, 702, 762
BstYI	RGATCY	5 cut(s)	105, 253, 439, 702, 762
BstZ17I	GTATAC	1 cut(s)	1428
Bsu36I	CCTNAGG	1 cut(s)	258
BsuI	GTATCC	1 cut(s)	766
BsuRI	GGCC	3 cut(s)	59, 1279, 2404
BtgI	CCRYGG	3 cut(s)	1282, 1435, 1876
BtsCI	GGATG	9 cut(s)	277, 673, 898, 1234, 1302, 1693, 1966, 2194, 2393
BtsI	GCAGTG	2 cut(s)	346, 583
BtsIMutI	CAGTG	6 cut(s)	240, 286, 346, 414, 583, 2376
BveI	ACCTGC	1 cut(s)	1435
Cac8I	GCNNGC	6 cut(s)	1065, 1078, 1590, 1640, 2057, 2105
CaiI	CAGNNNCTG	1 cut(s)	2081
CciI	TCATGA	3 cut(s)	1165, 1771, 2154
CfoI	GCGC	1 cut(s)	1111
Cfr10I	RCCGGY	1 cut(s)	2055
Cfr13I	GGNCC	4 cut(s)	191, 1277, 1278, 2403
CsiI	ACCWGGT	1 cut(s)	2226
Csp6I	GTAC	6 cut(s)	384, 824, 1030, 1087, 1184, 1693
CviQI	GTAC	6 cut(s)	384, 824, 1030, 1087, 1184, 1693
DdeI	CTNAG	6 cut(s)	258, 618, 903, 1971, 2090, 2163
DraIII	CACNNNGTG	1 cut(s)	578
Eam1104I	CTCTTC	1 cut(s)	870
EarI	CTCTTC	1 cut(s)	870
Ecl136II	GAGCTC	1 cut(s)	1672
Eco130I	CCWWGG	1 cut(s)	1435
Eco24I	GRGCYC	4 cut(s)	1165, 1281, 1545, 1674
Eco32I	GATATC	3 cut(s)	1289, 1937, 2096
Eco47I	GGWCC	1 cut(s)	191
Eco53kI	GAGCTC	1 cut(s)	1672
Eco57I	CTGAAG	1 cut(s)	765
Eco81I	CCTNAGG	1 cut(s)	258
Eco88I	CYCGRG	1 cut(s)	753
EcoICRI	GAGCTC	1 cut(s)	1672
EcoO109I	RGGNCCY	1 cut(s)	1278
EcoRI	GAATTC	1 cut(s)	989
EcoRII	CCWGG	6 cut(s)	184, 409, 610, 2026, 2226, 2293
EcoRV	GATATC	3 cut(s)	1289, 1937, 2096
EcoT14I	CCWWGG	1 cut(s)	1435
EcoT22I	ATGCAT	2 cut(s)	1213, 2130
EcoT38I	GRGCYC	4 cut(s)	1165, 1281, 1545, 1674
ErhI	CCWWGG	1 cut(s)	1435
FalI	AAGNNNNNCTT	2 cut(s)	2297, 2329
FaqI	GGGAC	2 cut(s)	62, 348
FauI	CCCGC	2 cut(s)	92, 742
FauNDI	CATATG	2 cut(s)	127, 1644
FbaI	TGATCA	1 cut(s)	2356
FblI	GTMKAC	2 cut(s)	276, 1427
Fnu4HI	GCNGC	5 cut(s)	162, 500, 1117, 2284, 2402
FokI	GGATG	9 cut(s)	284, 680, 885, 1241, 1309, 1700, 1953, 2201, 2400
FriOI	GRGCYC	4 cut(s)	1165, 1281, 1545, 1674
Fsp4HI	GCNGC	5 cut(s)	162, 500, 1117, 2284, 2402
FspBI	CTAG	4 cut(s)	266, 360, 1113, 2456
GlaI	GCGC	1 cut(s)	1110
GluI	GCNGC	5 cut(s)	162, 500, 1117, 2284, 2402
GsuI	CTGGAG	3 cut(s)	207, 633, 855
HaeIII	GGCC	3 cut(s)	59, 1279, 2404
HapII	CCGG	3 cut(s)	114, 460, 2056
HhaI	GCGC	1 cut(s)	1111
Hin6I	GCGC	1 cut(s)	1109
HinP1I	GCGC	1 cut(s)	1109
HincII	GTYRAC	1 cut(s)	2383
HindII	GTYRAC	1 cut(s)	2383
HindIII	AAGCTT	2 cut(s)	1022, 1078
HinfI	GANTC	9 cut(s)	131, 137, 245, 676, 794, 1304, 1534, 2318, 2327
HpaII	CCGG	3 cut(s)	114, 460, 2056
HphI	GGTGA	6 cut(s)	723, 1087, 1424, 1580, 1718, 1802
Hpy166II	GTNNAC	7 cut(s)	277, 570, 731, 1201, 1428, 1717, 2383
Hpy8I	GTNNAC	7 cut(s)	277, 570, 731, 1201, 1428, 1717, 2383
HpyAV	CCTTC	6 cut(s)	445, 518, 789, 1925, 2186, 2422
HpyCH4III	ACNGT	6 cut(s)	157, 281, 479, 1877, 2042, 2380
HpyCH4IV	ACGT	1 cut(s)	1223
HpyF10VI	GCNNNNNNNGC	5 cut(s)	96, 386, 614, 623, 1073
HpyF3I	CTNAG	6 cut(s)	258, 618, 903, 1971, 2090, 2163
HpySE526I	ACGT	1 cut(s)	1223
HspAI	GCGC	1 cut(s)	1109
KpnI	GGTACC	1 cut(s)	1090
KroI	GCCGGC	1 cut(s)	2055
KroNI	GCCGGC	1 cut(s)	2057
Ksp22I	TGATCA	1 cut(s)	2356
LmnI	GCTCC	5 cut(s)	238, 614, 835, 1060, 1677
Lsp1109I	GCAGC	4 cut(s)	148, 486, 1128, 2270
LweI	GCATC	7 cut(s)	635, 1220, 1489, 1506, 1572, 2068, 2137
MabI	ACCWGGT	1 cut(s)	2226
MaeI	CTAG	4 cut(s)	266, 360, 1113, 2456
MaeII	ACGT	1 cut(s)	1223
MaeIII	GTNAC	6 cut(s)	283, 1093, 1259, 1411, 1504, 1790
MbiI	CCGCTC	1 cut(s)	2401
MfeI	CAATTG	2 cut(s)	649, 1800
MflI	RGATCY	5 cut(s)	105, 253, 439, 702, 762
MhlI	GDGCHC	6 cut(s)	610, 1165, 1281, 1545, 1674, 2169
MlyI	GAGTC	3 cut(s)	670, 788, 2336
MmeI	TCCRAC	5 cut(s)	928, 1206, 1353, 1603, 2440
Mph1103I	ATGCAT	2 cut(s)	1213, 2130
MroNI	GCCGGC	1 cut(s)	2055
MroXI	GAANNNNTTC	2 cut(s)	121, 455
MseI	TTAA	9 cut(s)	92, 152, 368, 632, 975, 1017, 1383, 1524, 2288
MslI	CAYNNNNRTG	1 cut(s)	1206
MspA1I	CMGCKG	2 cut(s)	2366, 2441
MspI	CCGG	3 cut(s)	114, 460, 2056
MspR9I	CCNGG	7 cut(s)	186, 411, 461, 612, 2028, 2228, 2295
MunI	CAATTG	2 cut(s)	649, 1800
Mva1269I	GAATGC	3 cut(s)	667, 1590, 1805
MvaI	CCWGG	6 cut(s)	186, 411, 612, 2028, 2228, 2295
MvnI	CGCG	1 cut(s)	99
MwoI	GCNNNNNNNGC	5 cut(s)	96, 386, 614, 623, 1073
NaeI	GCCGGC	1 cut(s)	2057
NciI	CCSGG	1 cut(s)	461
NcoI	CCATGG	1 cut(s)	1435
NdeI	CATATG	2 cut(s)	127, 1644
NgoMIV	GCCGGC	1 cut(s)	2055
NlaIV	GGNNCC	7 cut(s)	255, 609, 831, 1088, 1252, 1279, 1280
NmuCI	GTSAC	3 cut(s)	1093, 1259, 1790
NsiI	ATGCAT	2 cut(s)	1213, 2130
NspI	RCATGY	4 cut(s)	88, 947, 1067, 1243
PaeI	GCATGC	1 cut(s)	1067
PaeR7I	CTCGAG	1 cut(s)	753
PagI	TCATGA	3 cut(s)	1165, 1771, 2154
PciI	ACATGT	3 cut(s)	84, 943, 1239
PctI	GAATGC	3 cut(s)	667, 1590, 1805
PdiI	GCCGGC	1 cut(s)	2057
PdmI	GAANNNNTTC	2 cut(s)	121, 455
PfeI	GAWTC	6 cut(s)	131, 137, 245, 1304, 1534, 2318
PflMI	CCANNNNNTGG	3 cut(s)	410, 851, 1684
PfoI	TCCNGGA	2 cut(s)	184, 459
PkrI	GCNGC	5 cut(s)	163, 501, 1118, 2285, 2403
PleI	GAGTC	3 cut(s)	670, 788, 2335
PpsI	GAGTC	3 cut(s)	670, 788, 2335
PscI	ACATGT	3 cut(s)	84, 943, 1239
PshBI	ATTAAT	1 cut(s)	632
Psp124BI	GAGCTC	1 cut(s)	1674
Psp6I	CCWGG	6 cut(s)	184, 409, 610, 2026, 2226, 2293
PspGI	CCWGG	6 cut(s)	184, 409, 610, 2026, 2226, 2293
PspN4I	GGNNCC	7 cut(s)	255, 609, 831, 1088, 1252, 1279, 1280
PspOMI	GGGCCC	1 cut(s)	1277
PspPI	GGNCC	4 cut(s)	191, 1277, 1278, 2403
PstNI	CAGNNNCTG	1 cut(s)	2081
PsuI	RGATCY	5 cut(s)	105, 253, 439, 702, 762
PvuII	CAGCTG	1 cut(s)	2441
RsaI	GTAC	6 cut(s)	385, 825, 1031, 1088, 1185, 1694
RsaNI	GTAC	6 cut(s)	384, 824, 1030, 1087, 1184, 1693
RseI	CAYNNNNRTG	1 cut(s)	1206
SacI	GAGCTC	1 cut(s)	1674
SaqAI	TTAA	9 cut(s)	92, 152, 368, 632, 975, 1017, 1383, 1524, 2288
SatI	GCNGC	5 cut(s)	162, 500, 1117, 2284, 2402
Sau96I	GGNCC	4 cut(s)	191, 1277, 1278, 2403
ScaI	AGTACT	2 cut(s)	385, 1694
SchI	GAGTC	3 cut(s)	670, 788, 2336
ScrFI	CCNGG	7 cut(s)	186, 411, 461, 612, 2028, 2228, 2295
SduI	GDGCHC	6 cut(s)	610, 1165, 1281, 1545, 1674, 2169
SexAI	ACCWGGT	1 cut(s)	2226
SfaNI	GCATC	7 cut(s)	635, 1220, 1489, 1506, 1572, 2068, 2137
SfcI	CTRYAG	2 cut(s)	1465, 1869
Sfr274I	CTCGAG	1 cut(s)	753
SinI	GGWCC	1 cut(s)	191
SlaI	CTCGAG	1 cut(s)	753
SmiMI	CAYNNNNRTG	1 cut(s)	1206
SmlI	CTYRAG	1 cut(s)	753
SmoI	CTYRAG	1 cut(s)	753
SphI	GCATGC	1 cut(s)	1067
SsiI	CCGC	9 cut(s)	99, 624, 665, 735, 928, 1617, 1763, 2364, 2401
SspI	AATATT	1 cut(s)	2334
SspMI	CTAG	4 cut(s)	266, 360, 1113, 2456
SstI	GAGCTC	1 cut(s)	1674
StyD4I	CCNGG	7 cut(s)	184, 409, 459, 610, 2026, 2226, 2293
StyI	CCWWGG	1 cut(s)	1435
TaaI	ACNGT	6 cut(s)	157, 281, 479, 1877, 2042, 2380
TaiI	ACGT	1 cut(s)	1226
TaqI	TCGA	4 cut(s)	471, 754, 2119, 2182
TatI	WGTACW	3 cut(s)	383, 1183, 1692
TauI	GCSGC	1 cut(s)	2404
TfiI	GAWTC	6 cut(s)	131, 137, 245, 1304, 1534, 2318
Tru1I	TTAA	9 cut(s)	92, 152, 368, 632, 975, 1017, 1383, 1524, 2288
Tru9I	TTAA	9 cut(s)	92, 152, 368, 632, 975, 1017, 1383, 1524, 2288
TscAI	CASTG	6 cut(s)	247, 286, 346, 421, 583, 2383
TseFI	GTSAC	3 cut(s)	1093, 1259, 1790
TseI	GCWGC	4 cut(s)	161, 499, 1116, 2283
Tsp45I	GTSAC	3 cut(s)	1093, 1259, 1790
TspGWI	ACGGA	2 cut(s)	755, 2400
TspRI	CASTG	6 cut(s)	247, 286, 346, 421, 583, 2383
Van91I	CCANNNNNTGG	3 cut(s)	410, 851, 1684
VpaK11BI	GGWCC	1 cut(s)	191
VspI	ATTAAT	1 cut(s)	632
XapI	RAATTY	7 cut(s)	174, 686, 989, 1379, 1942, 1958, 2111
XceI	RCATGY	4 cut(s)	88, 947, 1067, 1243
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1784
XhoI	CTCGAG	1 cut(s)	753
XmiI	GTMKAC	2 cut(s)	276, 1427
XmnI	GAANNNNTTC	2 cut(s)	121, 455
XspI	CTAG	4 cut(s)	266, 360, 1113, 2456
ZrmI	AGTACT	2 cut(s)	385, 1694
Zsp2I	ATGCAT	2 cut(s)	1213, 2130

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.