AT5G04000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006655
Physical Location & Seq
Reverse (-)
1079033 .. 1079899
867 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04000.1

Sequence Viewer

Length: 351 bp
ATGGAGCAAATTGCGGCGTTAGATGTCTCAAATGGTAAAGTAAAAGAGCCGAGGGTATTATCTCCGGGAGGGTCCAAGATCGAAGAGAGGCAAAGTCACAGTGCTACTAATGAGAATGATATGAGTCAAGATGGTAGAGATCAGCCTCATGGTGAAGTGAACATGGAAGCTTCTATATCAGCAGAAGATGTGATAAGAGCTGGAGGGTTTGGTGCTAAAGATGACATTGGAAGCTTCCTTCCTGTGGCGAGTGACTCGACTGACTTTGAGGAATCAATCCGATCTGCACGCGATTATGAAGAAGCGCAACCAGAGGTTCAAAGACCGGGTCTTGGCTATCCGAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

12.52

Weight (kDa)

4.48

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 291
AciI CCGC 1 cut(s) 14
AfiI CCNNNNNNNGG 2 cut(s) 244, 332
AgsI TTSAA 1 cut(s) 320
AluBI AGCT 3 cut(s) 170, 200, 234
AluI AGCT 3 cut(s) 170, 200, 234
Alw26I GTCTC 1 cut(s) 31
ArsI GACNNNNNNTTYG 2 cut(s) 313, 345
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 2 cut(s) 66, 327
AsuHPI GGTGA 1 cut(s) 164
AvaII GGWCC 1 cut(s) 72
BccI CCATC 1 cut(s) 125
BcnI CCSGG 2 cut(s) 66, 327
BcoDI GTCTC 1 cut(s) 31
BisI GCNGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 16
Bme1390I CCNGG 2 cut(s) 66, 327
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 73
BmrFI CCNGG 2 cut(s) 66, 327
BpmI CTGGAG 1 cut(s) 222
BpuMI CCSGG 2 cut(s) 66, 327
BsaJI CCNNGG 1 cut(s) 50
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 332
BseDI CCNNGG 1 cut(s) 50
BseLI CCNNNNNNNGG 2 cut(s) 244, 332
BsgI GTGCAG 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 291
BsiSI CCGG 2 cut(s) 65, 326
BslI CCNNNNNNNGG 2 cut(s) 244, 332
BsmAI GTCTC 1 cut(s) 31
Bsp143I GATC 3 cut(s) 78, 139, 281
BspACI CCGC 1 cut(s) 14
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 3 cut(s) 78, 139, 281
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 289
BstFNI CGCG 1 cut(s) 291
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 3 cut(s) 81, 142, 284
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 3 cut(s) 78, 139, 281
BstSCI CCNGG 2 cut(s) 64, 325
BstUI CGCG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 289
CfoI GCGC 1 cut(s) 307
Cfr13I GGNCC 1 cut(s) 72
CviAII CATG 2 cut(s) 149, 163
CviJI RGCY 6 cut(s) 49, 145, 170, 200, 234, 336
CviKI_1 RGCY 6 cut(s) 49, 145, 170, 200, 234, 336
DpnI GATC 3 cut(s) 80, 141, 283
DpnII GATC 3 cut(s) 78, 139, 281
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 72
FaeI CATG 2 cut(s) 152, 166
FaiI YATR 5 cut(s) 122, 150, 164, 176, 297
FatI CATG 2 cut(s) 148, 162
Fnu4HI GCNGC 1 cut(s) 15
Fsp4HI GCNGC 1 cut(s) 15
GlaI GCGC 1 cut(s) 306
GluI GCNGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 222
HapII CCGG 2 cut(s) 65, 326
HhaI GCGC 1 cut(s) 307
Hin1II CATG 2 cut(s) 152, 166
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HindIII AAGCTT 2 cut(s) 168, 232
HinfI GANTC 3 cut(s) 124, 254, 272
HpaII CCGG 2 cut(s) 65, 326
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 281, 342
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 248
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 1 cut(s) 287
Hsp92II CATG 2 cut(s) 152, 166
HspAI GCGC 1 cut(s) 305
Kzo9I GATC 3 cut(s) 78, 139, 281
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 78, 186, 255, 324, 339
MaeIII GTNAC 2 cut(s) 95, 251
MalI GATC 3 cut(s) 80, 141, 283
MboI GATC 3 cut(s) 78, 139, 281
MboII GAAGA 3 cut(s) 95, 197, 311
MluCI AATT 1 cut(s) 9
MlyI GAGTC 2 cut(s) 133, 248
MnlI CCTC 7 cut(s) 45, 62, 81, 156, 197, 262, 307
MspI CCGG 2 cut(s) 65, 326
MspR9I CCNGG 2 cut(s) 66, 327
MvnI CGCG 1 cut(s) 291
NciI CCSGG 2 cut(s) 66, 327
NdeII GATC 3 cut(s) 78, 139, 281
NlaIII CATG 2 cut(s) 152, 166
NlaIV GGNNCC 1 cut(s) 73
NmeAIII GCCGAG 1 cut(s) 75
NmuCI GTSAC 2 cut(s) 95, 251
PfeI GAWTC 1 cut(s) 272
PflFI GACNNNGTC 1 cut(s) 327
PfoI TCCNGGA 1 cut(s) 64
PkrI GCNGC 1 cut(s) 16
PleI GAGTC 2 cut(s) 132, 248
PpsI GAGTC 2 cut(s) 132, 248
PspN4I GGNNCC 1 cut(s) 73
PspPI GGNCC 1 cut(s) 72
PsyI GACNNNGTC 1 cut(s) 327
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 3 cut(s) 78, 139, 281
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 2 cut(s) 133, 248
ScrFI CCNGG 2 cut(s) 66, 327
SetI ASST 4 cut(s) 172, 202, 236, 318
SinI GGWCC 1 cut(s) 72
Sse9I AATT 1 cut(s) 9
SsiI CCGC 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 64, 325
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 2 cut(s) 81, 257
TasI AATT 1 cut(s) 9
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 272
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 2 cut(s) 95, 251
Tsp45I GTSAC 2 cut(s) 95, 251
TspDTI ATGAA 1 cut(s) 312
TspRI CASTG 1 cut(s) 106
Tth111I GACNNNGTC 1 cut(s) 327
VpaK11BI GGWCC 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.