Rorug03G0209400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006655
Physical Location & Seq
Reverse (-)
18646505 .. 18650411
3907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0209400.1

Sequence Viewer

Length: 1269 bp
ATGGGCAACGCTCAGTCACCTCCTCCCAAAGATCCTCGCTTTGCTTCCGCCTCCAGAGCTTTCACTCATACTGAGCTTGAAGATCTCAAGTCCCTGCTTGAGTCCTTGGCTGCTCAGTCACACAGCGACGGCCAGTTCATCTCTCCCTCGGTTTTTCAGACTTATTTCGGGCTTCATGGTCCTCTTGGAGATAGGATGTTTGATTTAGTAACCCAGCAACGGAAAGACCAGAAGCTCACTTTTGAAGACCTGGTGGTAGCCAAAGGGACTTATGAGAAGGGAACAAGAGATGATATTGAAGAGTTCATATATAAATTGTTGGACCTAAACGGCGATGGCATTTTGGGGAGGTCTGATCTGGAAGGTGTTCTGGTTGCAATGTTTGAATACATATTCAAAACAAAAAACTCTGAACCTGGATCAAGTACATATCGGGAAACTGTGAATGCATTTCTTAATGCTGCACAGTTTACAAAGCACGACGAAGGACATACTGAGGAAAGTTTGTCCTTTGAAGATTTCAGAACTTGGTGCACTCTTCTTCCTTCTGTCAGGAAGTTCCTTGGAAGCTTATTGATTCCACCTGATCAAGGAAGGCCAGGTTCTCAGGTTCCACGGTTATTGCAAGTGGAAAACATCAATTCAAATATGATATTATTAAACGAGGTGTATGCCTGGCATATAGGAGGAATCCTTCCTCACCAAGAGCTGGGAGAATGGAAACTATTATACCATAGTGCTGTTAATGGTCTGAGCTTCAACACATTTGTGGGAAACATATCAAATGATAAAGGGGCAACTGTATTCATTATCAAGGATAAAGACGGTTATGTATATGGAGGTTATGCTTCCCAACCGTGGGAGAGGCATGGTGATTTCTATGGTGATCTGAAGTCATTTCTTTTCCAATTGTATCCAAAGGCATCTATATATAGACCTACTGGAGCAAACACTAATCTACAATGGTGTGCTGTGAATTTCGGTTCAGAGAGCATCCCAAATGGTATTGGTTTTGGAGGAAAGGTGAATCACTTTGGTCTCTTCCTTTCGGCGAACTTTGATCAGGGGCACACTTTCTCGTGTACCACATTTGGTTCCCCTTGCCTCTCCAAGACCAGCCGAATATGCCCGGAAGTAATAGAATGCTGGGGAGTTGTTAGGAAAGCAGCAGATGAAGAAAAACAGGACGGTGCTAAAGGCACTGTATTGGAGAGGTTTAAGGAAGATCGTCATATGCTCAACATGGTTGGGCTCGCAAATTCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

47.02

Weight (kDa)

5.59

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TLD PF07534 215 - 385 1.3e-41 TLDc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 709
AciI CCGC 1 cut(s) 48
AclWI GGATC 2 cut(s) 26, 427
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 2 cut(s) 976, 1258
AcuI CTGAAG 1 cut(s) 911
AfaI GTAC 2 cut(s) 427, 1084
AfiI CCNNNNNNNGG 5 cut(s) 219, 590, 709, 858, 859
AgsI TTSAA 8 cut(s) 80, 245, 299, 386, 397, 515, 645, 760
AjnI CCWGG 4 cut(s) 249, 415, 598, 674
AleI CACNNNNGTG 1 cut(s) 767
AluBI AGCT 7 cut(s) 59, 76, 235, 570, 709, 756, 1266
AluI AGCT 7 cut(s) 59, 76, 235, 570, 709, 756, 1266
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 1 cut(s) 1043
Alw44I GTGCAC 1 cut(s) 532
AlwI GGATC 2 cut(s) 26, 427
AoxI GGCC 2 cut(s) 130, 596
ApaLI GTGCAC 1 cut(s) 532
ApeKI GCWGC 3 cut(s) 110, 461, 1166
ApoI RAATTY 2 cut(s) 976, 1258
Asp700I GAANNNNTTC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 179, 322
AsuC2I CCSGG 1 cut(s) 1130
AsuHPI GGTGA 5 cut(s) 9, 692, 884, 896, 1036
AvaII GGWCC 2 cut(s) 179, 322
BaeGI GKGCMC 2 cut(s) 536, 1071
BanII GRGCYC 1 cut(s) 1254
BauI CACGAG 1 cut(s) 1078
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 536
BbvI GCAGC 3 cut(s) 97, 448, 1178
BccI CCATC 1 cut(s) 329
BceAI ACGGC 2 cut(s) 145, 346
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 4 cut(s) 251, 417, 600, 676
BciVI GTATCC 1 cut(s) 924
BclI TGATCA 2 cut(s) 586, 1060
BcnI CCSGG 1 cut(s) 1130
BcoDI GTCTC 1 cut(s) 1043
BfaI CTAG 1 cut(s) 1267
BfuI GTATCC 1 cut(s) 924
BglII AGATCT 1 cut(s) 82
BisI GCNGC 3 cut(s) 111, 462, 1167
BlsI GCNGC 3 cut(s) 112, 463, 1168
Bme1390I CCNGG 5 cut(s) 251, 417, 600, 676, 1130
Bme18I GGWCC 2 cut(s) 179, 322
BmgT120I GGNCC 2 cut(s) 179, 322
BmiI GGNNCC 2 cut(s) 612, 1096
BmrFI CCNGG 5 cut(s) 251, 417, 600, 676, 1130
BmsI GCATC 2 cut(s) 932, 1002
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 2 cut(s) 37, 963
BpuEI CTTGAG 2 cut(s) 71, 119
BpuMI CCSGG 1 cut(s) 1130
BsaI GGTCTC 1 cut(s) 1043
BsaJI CCNNGG 5 cut(s) 105, 147, 562, 614, 857
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 5 cut(s) 219, 590, 709, 858, 859
Bse1I ACTGG 2 cut(s) 133, 946
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 4 cut(s) 251, 417, 600, 676
BseDI CCNNGG 5 cut(s) 105, 147, 562, 614, 857
BseGI GGATG 2 cut(s) 201, 993
BseLI CCNNNNNNNGG 5 cut(s) 219, 590, 709, 858, 859
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 6 cut(s) 26, 63, 128, 486, 620, 743
BseNI ACTGG 2 cut(s) 133, 946
BseRI GAGGAG 1 cut(s) 12
BseSI GKGCMC 2 cut(s) 536, 1071
BseXI GCAGC 3 cut(s) 97, 448, 1178
BseYI CCCAGC 3 cut(s) 213, 709, 1146
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 2 cut(s) 132, 598
BsiHKAI GWGCWC 1 cut(s) 536
BsiSI CCGG 1 cut(s) 1130
BslFI GGGAC 2 cut(s) 76, 280
BslI CCNNNNNNNGG 5 cut(s) 219, 590, 709, 858, 859
BsmAI GTCTC 1 cut(s) 1043
BsmFI GGGAC 2 cut(s) 76, 280
BsmI GAATGC 2 cut(s) 451, 1148
BsnI GGCC 2 cut(s) 132, 598
Bso31I GGTCTC 1 cut(s) 1043
Bsp1286I GDGCHC 3 cut(s) 536, 1071, 1254
Bsp143I GATC 8 cut(s) 31, 82, 355, 419, 586, 886, 1060, 1225
BspACI CCGC 1 cut(s) 48
BspANI GGCC 2 cut(s) 132, 598
BspCNI CTCAG 6 cut(s) 25, 64, 127, 487, 619, 744
BspLI GGNNCC 2 cut(s) 612, 1096
BspPI GGATC 2 cut(s) 26, 427
BspTNI GGTCTC 1 cut(s) 1043
BsrDI GCAATG 1 cut(s) 384
BsrI ACTGG 2 cut(s) 133, 946
BssECI CCNNGG 5 cut(s) 105, 147, 562, 614, 857
BssMI GATC 8 cut(s) 31, 82, 355, 419, 586, 886, 1060, 1225
BssSI CACGAG 1 cut(s) 1078
BssT1I CCWWGG 2 cut(s) 105, 562
Bst2BI CACGAG 1 cut(s) 1078
Bst2UI CCWGG 4 cut(s) 251, 417, 600, 676
Bst4CI ACNGT 8 cut(s) 442, 468, 618, 802, 827, 858, 1190, 1204
Bst6I CTCTTC 3 cut(s) 294, 543, 1046
BstC8I GCNNGC 1 cut(s) 1254
BstDEI CTNAG 6 cut(s) 12, 72, 114, 495, 606, 752
BstDSI CCRYGG 2 cut(s) 614, 857
BstENI CCTNNNNNAGG 1 cut(s) 588
BstF5I GGATG 2 cut(s) 201, 993
BstKTI GATC 8 cut(s) 34, 85, 358, 422, 589, 889, 1063, 1228
BstMAI GTCTC 1 cut(s) 1043
BstMBI GATC 8 cut(s) 31, 82, 355, 419, 586, 886, 1060, 1225
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1125
BstNI CCWGG 4 cut(s) 251, 417, 600, 676
BstSCI CCNGG 5 cut(s) 249, 415, 598, 674, 1128
BstSLI GKGCMC 2 cut(s) 536, 1071
BstV1I GCAGC 3 cut(s) 97, 448, 1178
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 2 cut(s) 31, 82
BstYI RGATCY 2 cut(s) 31, 82
BsuI GTATCC 1 cut(s) 924
BsuRI GGCC 2 cut(s) 132, 598
BtgI CCRYGG 2 cut(s) 614, 857
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 2 cut(s) 201, 993
BtsIMutI CAGTG 1 cut(s) 1200
Cac8I GCNNGC 1 cut(s) 1254
Cfr13I GGNCC 2 cut(s) 179, 322
CsiI ACCWGGT 1 cut(s) 249
Csp6I GTAC 2 cut(s) 426, 1083
CspCI CAANNNNNGTGG 2 cut(s) 603, 638
CviAII CATG 3 cut(s) 176, 869, 1243
CviQI GTAC 2 cut(s) 426, 1083
DdeI CTNAG 6 cut(s) 12, 72, 114, 495, 606, 752
DpnI GATC 8 cut(s) 33, 84, 357, 421, 588, 888, 1062, 1227
DpnII GATC 8 cut(s) 31, 82, 355, 419, 586, 886, 1060, 1225
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 3 cut(s) 294, 543, 1046
EarI CTCTTC 3 cut(s) 294, 543, 1046
EciI GGCGGA 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 105, 562
Eco24I GRGCYC 1 cut(s) 1254
Eco31I GGTCTC 1 cut(s) 1043
Eco47I GGWCC 2 cut(s) 179, 322
Eco57I CTGAAG 1 cut(s) 911
EcoNI CCTNNNNNAGG 1 cut(s) 588
EcoRII CCWGG 4 cut(s) 249, 415, 598, 674
EcoT14I CCWWGG 2 cut(s) 105, 562
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 1254
ErhI CCWWGG 2 cut(s) 105, 562
FaeI CATG 3 cut(s) 179, 872, 1246
FaqI GGGAC 2 cut(s) 76, 280
FatI CATG 3 cut(s) 175, 868, 1242
FauNDI CATATG 1 cut(s) 1233
FbaI TGATCA 2 cut(s) 586, 1060
Fnu4HI GCNGC 3 cut(s) 111, 462, 1167
FokI GGATG 2 cut(s) 208, 980
FriOI GRGCYC 1 cut(s) 1254
Fsp4HI GCNGC 3 cut(s) 111, 462, 1167
FspBI CTAG 1 cut(s) 1267
GluI GCNGC 3 cut(s) 111, 462, 1167
GsaI CCCAGC 3 cut(s) 217, 713, 1150
GsuI CTGGAG 2 cut(s) 37, 963
HaeIII GGCC 2 cut(s) 132, 598
HapII CCGG 1 cut(s) 1130
Hin1II CATG 3 cut(s) 179, 872, 1246
HindIII AAGCTT 1 cut(s) 568
HinfI GANTC 4 cut(s) 101, 577, 690, 1027
HpaII CCGG 1 cut(s) 1130
HphI GGTGA 5 cut(s) 9, 692, 884, 896, 1036
Hpy166II GTNNAC 3 cut(s) 471, 534, 1083
Hpy188I TCNGA 7 cut(s) 159, 355, 412, 524, 753, 891, 988
Hpy188III TCNNGA 4 cut(s) 54, 359, 434, 553
Hpy8I GTNNAC 3 cut(s) 471, 534, 1083
Hpy99I CGWCG 2 cut(s) 131, 485
HpyAV CCTTC 6 cut(s) 271, 356, 479, 555, 588, 704
HpyCH4III ACNGT 8 cut(s) 442, 468, 618, 802, 827, 858, 1190, 1204
HpyCH4V TGCA 5 cut(s) 377, 449, 464, 534, 625
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1125
HpyF3I CTNAG 6 cut(s) 12, 72, 114, 495, 606, 752
Hsp92II CATG 3 cut(s) 179, 872, 1246
Ksp22I TGATCA 2 cut(s) 586, 1060
Kzo9I GATC 8 cut(s) 31, 82, 355, 419, 586, 886, 1060, 1225
LmnI GCTCC 1 cut(s) 944
Lsp1109I GCAGC 3 cut(s) 97, 448, 1178
LweI GCATC 2 cut(s) 932, 1002
MabI ACCWGGT 1 cut(s) 249
MaeI CTAG 1 cut(s) 1267
MaeIII GTNAC 3 cut(s) 15, 117, 208
MalI GATC 8 cut(s) 33, 84, 357, 421, 588, 888, 1062, 1227
MboI GATC 8 cut(s) 31, 82, 355, 419, 586, 886, 1060, 1225
MboII GAAGA 9 cut(s) 92, 257, 311, 527, 530, 533, 1033, 1187, 1235
MfeI CAATTG 1 cut(s) 908
MflI RGATCY 2 cut(s) 31, 82
MhlI GDGCHC 3 cut(s) 536, 1071, 1254
MluCI AATT 5 cut(s) 314, 640, 908, 976, 1258
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 451
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 4 cut(s) 456, 659, 744, 1218
MslI CAYNNNNRTG 1 cut(s) 767
MspI CCGG 1 cut(s) 1130
MspR9I CCNGG 5 cut(s) 251, 417, 600, 676, 1130
MunI CAATTG 1 cut(s) 908
Mva1269I GAATGC 2 cut(s) 451, 1148
MvaI CCWGG 4 cut(s) 251, 417, 600, 676
MwoI GCNNNNNNNGC 2 cut(s) 56, 1125
NciI CCSGG 1 cut(s) 1130
NdeI CATATG 1 cut(s) 1233
NdeII GATC 8 cut(s) 31, 82, 355, 419, 586, 886, 1060, 1225
NlaIII CATG 3 cut(s) 179, 872, 1246
NlaIV GGNNCC 2 cut(s) 612, 1096
NmuCI GTSAC 2 cut(s) 15, 117
NsiI ATGCAT 1 cut(s) 451
OliI CACNNNNGTG 1 cut(s) 767
PctI GAATGC 2 cut(s) 451, 1148
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 3 cut(s) 577, 690, 1027
PflMI CCANNNNNTGG 1 cut(s) 709
PkrI GCNGC 3 cut(s) 112, 463, 1168
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
Psp6I CCWGG 4 cut(s) 249, 415, 598, 674
PspFI CCCAGC 3 cut(s) 213, 709, 1146
PspGI CCWGG 4 cut(s) 249, 415, 598, 674
PspN4I GGNNCC 2 cut(s) 612, 1096
PspPI GGNCC 2 cut(s) 179, 322
PsuI RGATCY 2 cut(s) 31, 82
RsaI GTAC 2 cut(s) 427, 1084
RsaNI GTAC 2 cut(s) 426, 1083
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 4 cut(s) 456, 659, 744, 1218
SatI GCNGC 3 cut(s) 111, 462, 1167
Sau3AI GATC 8 cut(s) 31, 82, 355, 419, 586, 886, 1060, 1225
Sau96I GGNCC 2 cut(s) 179, 322
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 5 cut(s) 251, 417, 600, 676, 1130
SduI GDGCHC 3 cut(s) 536, 1071, 1254
SexAI ACCWGGT 1 cut(s) 249
SfaNI GCATC 2 cut(s) 932, 1002
SinI GGWCC 2 cut(s) 179, 322
SmiMI CAYNNNNRTG 1 cut(s) 767
SmlI CTYRAG 2 cut(s) 86, 98
SmoI CTYRAG 2 cut(s) 86, 98
Sse9I AATT 5 cut(s) 314, 640, 908, 976, 1258
SsiI CCGC 1 cut(s) 48
SspMI CTAG 1 cut(s) 1267
StyD4I CCNGG 5 cut(s) 249, 415, 598, 674, 1128
StyI CCWWGG 2 cut(s) 105, 562
TaaI ACNGT 8 cut(s) 442, 468, 618, 802, 827, 858, 1190, 1204
TasI AATT 5 cut(s) 314, 640, 908, 976, 1258
TatI WGTACW 1 cut(s) 425
TfiI GAWTC 3 cut(s) 577, 690, 1027
Tru1I TTAA 4 cut(s) 456, 659, 744, 1218
Tru9I TTAA 4 cut(s) 456, 659, 744, 1218
TscAI CASTG 1 cut(s) 1207
TseFI GTSAC 2 cut(s) 15, 117
TseI GCWGC 3 cut(s) 110, 461, 1166
Tsp45I GTSAC 2 cut(s) 15, 117
TspDTI ATGAA 5 cut(s) 127, 164, 295, 796, 1188
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 1 cut(s) 1207
Van91I CCANNNNNTGG 1 cut(s) 709
VneI GTGCAC 1 cut(s) 532
VpaK11BI GGWCC 2 cut(s) 179, 322
XagI CCTNNNNNAGG 1 cut(s) 588
XapI RAATTY 2 cut(s) 976, 1258
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 1 cut(s) 1267
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.