Rmu_sc0003477.1_g000017

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003477.1
OG0006655
Physical Location & Seq
Reverse (-)
104956 .. 112506
7551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003477.1_g000017.1.cds

Sequence Viewer

Length: 771 bp
atgggctaccaccagcgcaacctgaaggcctcaccaaacgtgtcctacaagtcagatgacacgttgaactttcgtgacgctgatatgcaaaatatcctttccatccatccctctcgattttcaattgcaaagcagagcagagctactctcttcctgaatcccactcccgcttccaggtttgttttggaagaaacattgaaggcccttatagtgaagagaattgcaaaatttgatccaacatggagcgcaagcaggatgaagttctgcatgccggttcggtttatttcggccggttccgacccggttggatccggtccggtgcggtccactgtatctttcttaactgtttcttccggttcggtaaccggaacccattttccagtccggctcggccggttttcacctcacgataaggatgaaaaccagacaaggaatgaggaaggtgtaacatatcaaattcctgttttgaaaccgttctcaagttctccagatattgaagtgcaattagctaatgcggttgattggggaggtggaaaacacattgtaccttctggagatatcgatgcctctggtgaagtaaacacggaggcatcaataacacctgatgacgttatacgggctggaggctttggtgctagagatgatataagtagttttcttcctgtcgcgagtgattctactgattttgaagctaccatacgtgatgcccgagactctgaagaaccacagcgagatatctgtagaccaggtcttggctggacgaaaagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

256

Amino Acids

27.96

Weight (kDa)

5.97

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 752
AccI GTMKAC 1 cut(s) 742
AccII CGCG 1 cut(s) 668
AciI CCGC 3 cut(s) 168, 322, 515
AclWI GGATC 3 cut(s) 227, 303, 316
AcoI YGGCCR 2 cut(s) 288, 391
AcsI RAATTY 2 cut(s) 227, 456
AcuI CTGAAG 2 cut(s) 44, 738
AfaI GTAC 1 cut(s) 546
AfiI CCNNNNNNNGG 2 cut(s) 174, 752
AflIII ACRYGT 2 cut(s) 39, 60
AgsI TTSAA 6 cut(s) 67, 123, 199, 469, 497, 689
AjnI CCWGG 2 cut(s) 173, 745
AloI GAACNNNNNNTCC 2 cut(s) 361, 393
AluBI AGCT 4 cut(s) 143, 509, 692, 768
AluI AGCT 4 cut(s) 143, 509, 692, 768
Alw26I GTCTC 1 cut(s) 705
AlwI GGATC 3 cut(s) 227, 303, 316
Ama87I CYCGRG 1 cut(s) 708
AoxI GGCC 4 cut(s) 27, 201, 288, 391
ApoI RAATTY 2 cut(s) 227, 456
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 2 cut(s) 18, 248
AspS9I GGNCC 3 cut(s) 202, 314, 324
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 3 cut(s) 24, 393, 584
AvaI CYCGRG 1 cut(s) 708
AvaII GGWCC 2 cut(s) 314, 324
BamHI GGATCC 1 cut(s) 308
BarI GAAGNNNNNNTAC 2 cut(s) 681, 713
BccI CCATC 2 cut(s) 110, 114
BciT130I CCWGG 2 cut(s) 175, 747
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 705
BfaI CTAG 2 cut(s) 636, 769
BfmI CTRYAG 1 cut(s) 739
Bme1390I CCNGG 3 cut(s) 175, 302, 747
Bme18I GGWCC 2 cut(s) 314, 324
BmeT110I CYCGRG 1 cut(s) 708
BmgT120I GGNCC 3 cut(s) 202, 314, 324
BmiI GGNNCC 3 cut(s) 295, 310, 370
BmrFI CCNGG 3 cut(s) 175, 302, 747
BmsI GCATC 3 cut(s) 553, 599, 694
BplI GAGNNNNNCTC 2 cut(s) 132, 164
BpmI CTGGAG 3 cut(s) 471, 573, 642
BpuEI CTTGAG 1 cut(s) 463
BpuMI CCSGG 1 cut(s) 302
Bsa29I ATCGAT 1 cut(s) 561
BsaAI YACGTR 1 cut(s) 701
BsaWI WCCGGW 4 cut(s) 311, 316, 353, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 752
Bse118I RCCGGY 3 cut(s) 271, 290, 393
Bse1I ACTGG 1 cut(s) 380
BseBI CCWGG 2 cut(s) 175, 747
BseCI ATCGAT 1 cut(s) 561
BseGI GGATG 4 cut(s) 102, 106, 261, 421
BseLI CCNNNNNNNGG 2 cut(s) 174, 752
BseNI ACTGG 1 cut(s) 380
BseX3I CGGCCG 2 cut(s) 288, 391
Bsh1236I CGCG 1 cut(s) 668
Bsh1285I CGRYCG 2 cut(s) 291, 394
BshFI GGCC 4 cut(s) 29, 203, 290, 393
BshVI ATCGAT 1 cut(s) 561
BsiEI CGRYCG 2 cut(s) 291, 394
BsiHKCI CYCGRG 1 cut(s) 708
BsiSI CCGG 9 cut(s) 272, 291, 302, 312, 317, 354, 366, 385, 394
BslI CCNNNNNNNGG 2 cut(s) 174, 752
BsmAI GTCTC 1 cut(s) 705
BsnI GGCC 4 cut(s) 29, 203, 290, 393
BsoBI CYCGRG 1 cut(s) 708
Bsp143I GATC 2 cut(s) 232, 308
Bsp68I TCGCGA 1 cut(s) 668
BspACI CCGC 3 cut(s) 168, 322, 515
BspANI GGCC 4 cut(s) 29, 203, 290, 393
BspDI ATCGAT 1 cut(s) 561
BspFNI CGCG 1 cut(s) 668
BspLI GGNNCC 3 cut(s) 295, 310, 370
BspPI GGATC 3 cut(s) 227, 303, 316
BsrFI RCCGGY 3 cut(s) 271, 290, 393
BsrI ACTGG 1 cut(s) 380
BssAI RCCGGY 3 cut(s) 271, 290, 393
BssMI GATC 2 cut(s) 232, 308
Bst2UI CCWGG 2 cut(s) 175, 747
Bst4CI ACNGT 3 cut(s) 331, 346, 474
Bst6I CTCTTC 2 cut(s) 155, 209
BstBAI YACGTR 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 250, 269
BstEII GGTNACC 1 cut(s) 361
BstF5I GGATG 4 cut(s) 102, 106, 261, 421
BstFNI CGCG 1 cut(s) 668
BstHHI GCGC 2 cut(s) 18, 248
BstKTI GATC 2 cut(s) 235, 311
BstMAI GTCTC 1 cut(s) 705
BstMBI GATC 2 cut(s) 232, 308
BstMCI CGRYCG 2 cut(s) 291, 394
BstNI CCWGG 2 cut(s) 175, 747
BstNSI RCATGY 1 cut(s) 271
BstPI GGTNACC 1 cut(s) 361
BstSCI CCNGG 3 cut(s) 173, 300, 745
BstSFI CTRYAG 1 cut(s) 739
BstUI CGCG 1 cut(s) 668
BstX2I RGATCY 1 cut(s) 308
BstYI RGATCY 1 cut(s) 308
BstZI CGGCCG 2 cut(s) 288, 391
Bsu15I ATCGAT 1 cut(s) 561
BsuRI GGCC 4 cut(s) 29, 203, 290, 393
BsuTUI ATCGAT 1 cut(s) 561
BtsCI GGATG 4 cut(s) 102, 106, 261, 421
BtsIMutI CAGTG 1 cut(s) 327
BtuMI TCGCGA 1 cut(s) 668
Cac8I GCNNGC 2 cut(s) 250, 269
CfoI GCGC 2 cut(s) 18, 248
Cfr10I RCCGGY 3 cut(s) 271, 290, 393
Cfr13I GGNCC 3 cut(s) 202, 314, 324
ClaI ATCGAT 1 cut(s) 561
CpoI CGGWCCG 1 cut(s) 314
CseI GACGC 1 cut(s) 86
CsiI ACCWGGT 1 cut(s) 745
Csp6I GTAC 1 cut(s) 545
CspI CGGWCCG 1 cut(s) 314
CviAII CATG 2 cut(s) 240, 268
CviQI GTAC 1 cut(s) 545
DpnI GATC 2 cut(s) 234, 310
DpnII GATC 2 cut(s) 232, 308
EaeI YGGCCR 2 cut(s) 288, 391
EagI CGGCCG 2 cut(s) 288, 391
Eam1104I CTCTTC 2 cut(s) 155, 209
EarI CTCTTC 2 cut(s) 155, 209
EclXI CGGCCG 2 cut(s) 288, 391
Eco147I AGGCCT 1 cut(s) 29
Eco32I GATATC 2 cut(s) 559, 736
Eco47I GGWCC 2 cut(s) 314, 324
Eco52I CGGCCG 2 cut(s) 288, 391
Eco57I CTGAAG 2 cut(s) 44, 738
Eco88I CYCGRG 1 cut(s) 708
Eco91I GGTNACC 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 361
EcoRII CCWGG 2 cut(s) 173, 745
EcoRV GATATC 2 cut(s) 559, 736
FaeI CATG 2 cut(s) 243, 271
FaiI YATR 8 cut(s) 86, 209, 241, 269, 451, 614, 647, 698
FatI CATG 2 cut(s) 239, 267
FauI CCCGC 1 cut(s) 175
FblI GTMKAC 1 cut(s) 742
FokI GGATG 4 cut(s) 89, 93, 268, 428
FspBI CTAG 2 cut(s) 636, 769
GlaI GCGC 2 cut(s) 17, 247
GsuI CTGGAG 3 cut(s) 471, 573, 642
HaeIII GGCC 4 cut(s) 29, 203, 290, 393
HapII CCGG 9 cut(s) 272, 291, 302, 312, 317, 354, 366, 385, 394
HgaI GACGC 1 cut(s) 86
HhaI GCGC 2 cut(s) 18, 248
Hin1II CATG 2 cut(s) 243, 271
Hin6I GCGC 2 cut(s) 16, 246
HinP1I GCGC 2 cut(s) 16, 246
HinfI GANTC 3 cut(s) 157, 674, 713
HpaII CCGG 9 cut(s) 272, 291, 302, 312, 317, 354, 366, 385, 394
HphI GGTGA 3 cut(s) 24, 393, 584
Hpy166II GTNNAC 3 cut(s) 327, 580, 743
Hpy188I TCNGA 3 cut(s) 55, 298, 718
Hpy188III TCNNGA 7 cut(s) 74, 114, 154, 407, 488, 552, 667
Hpy8I GTNNAC 3 cut(s) 327, 580, 743
HpyAV CCTTC 4 cut(s) 19, 193, 434, 558
HpyCH4III ACNGT 3 cut(s) 331, 346, 474
HpyCH4IV ACGT 4 cut(s) 39, 62, 609, 700
HpyCH4V TGCA 5 cut(s) 88, 128, 224, 267, 502
HpySE526I ACGT 4 cut(s) 39, 62, 609, 700
Hsp92II CATG 2 cut(s) 243, 271
HspAI GCGC 2 cut(s) 16, 246
Kzo9I GATC 2 cut(s) 232, 308
LmnI GCTCC 1 cut(s) 243
LweI GCATC 3 cut(s) 553, 599, 694
MabI ACCWGGT 1 cut(s) 745
MaeI CTAG 2 cut(s) 636, 769
MaeII ACGT 4 cut(s) 39, 62, 609, 700
MaeIII GTNAC 3 cut(s) 74, 361, 445
MalI GATC 2 cut(s) 234, 310
MboI GATC 2 cut(s) 232, 308
MboII GAAGA 6 cut(s) 142, 200, 226, 342, 650, 731
MfeI CAATTG 1 cut(s) 123
MflI RGATCY 1 cut(s) 308
MluCI AATT 5 cut(s) 123, 219, 227, 456, 503
MlyI GAGTC 1 cut(s) 707
MmeI TCCRAC 3 cut(s) 260, 286, 321
MnlI CCTC 8 cut(s) 40, 121, 414, 430, 521, 577, 580, 617
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 1 cut(s) 341
MspI CCGG 9 cut(s) 272, 291, 302, 312, 317, 354, 366, 385, 394
MspR9I CCNGG 3 cut(s) 175, 302, 747
MunI CAATTG 1 cut(s) 123
MvaI CCWGG 2 cut(s) 175, 747
MvnI CGCG 1 cut(s) 668
NciI CCSGG 1 cut(s) 302
NdeII GATC 2 cut(s) 232, 308
NlaIII CATG 2 cut(s) 243, 271
NlaIV GGNNCC 3 cut(s) 295, 310, 370
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 74
NruI TCGCGA 1 cut(s) 668
NspI RCATGY 1 cut(s) 271
PaeI GCATGC 1 cut(s) 271
PceI AGGCCT 1 cut(s) 29
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 157, 674
PflFI GACNNNGTC 1 cut(s) 747
PflMI CCANNNNNTGG 1 cut(s) 752
PleI GAGTC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 707
Ppu21I YACGTR 1 cut(s) 701
Psp6I CCWGG 2 cut(s) 173, 745
PspEI GGTNACC 1 cut(s) 361
PspGI CCWGG 2 cut(s) 173, 745
PspN4I GGNNCC 3 cut(s) 295, 310, 370
PspPI GGNCC 3 cut(s) 202, 314, 324
PsuI RGATCY 1 cut(s) 308
PsyI GACNNNGTC 1 cut(s) 747
RruI TCGCGA 1 cut(s) 668
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
Rsr2I CGGWCCG 1 cut(s) 314
RsrII CGGWCCG 1 cut(s) 314
SaqAI TTAA 1 cut(s) 341
Sau3AI GATC 2 cut(s) 232, 308
Sau96I GGNCC 3 cut(s) 202, 314, 324
SchI GAGTC 1 cut(s) 707
ScrFI CCNGG 3 cut(s) 175, 302, 747
SexAI ACCWGGT 1 cut(s) 745
SfaNI GCATC 3 cut(s) 553, 599, 694
SfcI CTRYAG 1 cut(s) 739
SinI GGWCC 2 cut(s) 314, 324
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
SphI GCATGC 1 cut(s) 271
Sse9I AATT 5 cut(s) 123, 219, 227, 456, 503
SseBI AGGCCT 1 cut(s) 29
SsiI CCGC 3 cut(s) 168, 322, 515
SspMI CTAG 2 cut(s) 636, 769
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 173, 300, 745
TaaI ACNGT 3 cut(s) 331, 346, 474
TaiI ACGT 4 cut(s) 42, 65, 612, 703
TaqI TCGA 2 cut(s) 115, 561
TasI AATT 5 cut(s) 123, 219, 227, 456, 503
TfiI GAWTC 2 cut(s) 157, 674
Tru1I TTAA 1 cut(s) 341
Tru9I TTAA 1 cut(s) 341
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 2 cut(s) 272, 432
TspGWI ACGGA 1 cut(s) 599
TspRI CASTG 1 cut(s) 334
Tth111I GACNNNGTC 1 cut(s) 747
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 2 cut(s) 314, 324
XapI RAATTY 2 cut(s) 227, 456
XceI RCATGY 1 cut(s) 271
XcmI CCANNNNNNNNNTGG 2 cut(s) 181, 753
XmiI GTMKAC 1 cut(s) 742
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 2 cut(s) 636, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.