AT5G04290

Early transcription elongation factor of RNA pol II, NGN section

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012966
Physical Location & Seq
Forward (+)
1195969 .. 1202936
6968 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04290.1

Sequence Viewer

Length: 4482 bp
ATGGATCGCAAGGGAAAGGGTAAACAGGTCGCCGGTAGTGATTCGTATTCCGGCGGACAGAAAAGGAAAAACTCCGTCGAATTCAGAGATGAAGGTTTGAGGATTAAGAAGCGCAAAAACCCTGAGGTTCTTCAGTTCTTCGAAGAATCAGCTGAAGTTGGCTACTACGGAGGCAGCTCCGACGAAGATGATGACGGATTAGGTTTCCTTAACGATATGGAAGACGAGCCTGAGGTGGAAGAGAGTAGTAAGGCTGGCAAGGGAGAAAAGGGCAAATCATCCTTTGTTTTTCCCAAGGAAGAAGATTTAAACGAGGAGGAATTTGATAGAATAATGGAAGAAAGATACAAGCCAGGTTCTGGTTTTCTTAGATATGCTGATGATGATATCAAAGATGCCATTGAGATGGATGCTCTTGCTCCAACCTCCAAGGATCCTCCTATATGGAAAGTCAAGTGTGCGATTGGACGGGAGAGGCGATCCGTTTTCTGTCTCATGCACAAATTTGTTGAGCTGAGAAAGATAGGCACCAAATTGGAGATCATATCTGTTTTTTCTGTGGACCATGTCAAAGGTTTCATTTTCATAGAAGCGGACAAGGAACATGATGTTCTTGAGGCATGCAAGAGTTTAGTTGGTATATACGCTACTCGGATGGTGTTACTTCCTAAAGCCGAAACTCCAAATCTGCTAACTGTCCAAAAAAAAACTAAAAAAGTTTCTGAGGGAACATGGGCTCGTGTTAAGAATGGTAAATACAAAGGAGATCTTGCTCAGATTGTGGCTGTGAGTGACACTCGGAATAAAGCACTAATAAAGCTAATTCCGAGGATTGATATTCAGGCCTTGACCCAGAAATATGGTGGGGGAGTTACTGTCCAAAAAGGCCAGACTCCAGCTCCAAGATTGATCAGTTCAAGCGAGCTCGAGGAGTTCAGACCTCTCATTCAAGTGAGGCGAGATCGTGATACTGGAATAACTTTTGAACATCTTGATAGTCTGATGCTGAAAGATGGGTATCTCTATAAGAAAGTATCCTTAGATTCAATATCTTCCTGGGGGGTTATACCAACGAAGGATGAGTTACTAAAATTTACGCCTGTAGACCGAAAAGAAACTGGTGATGTAGAGTGGATCTCAGAAATTTATGGTGAAGAAAGGAAGAAAAAGATTCTTCCAACCTGCAGAGAGGGTGGGAAAGGAGAAGGATCAGGGGGTGGAAAAGGAGAAGGATCAGGGGGTGGAAAAGGAGAAGGGTCAAGGGGTGGAAAAGGAGAAGGTTCATCAGATTTCAAGTCAGAAAGTAGCTATGAGTTATATAATCTAGTTTGTTTCAGTCGAAAAGACTTTGGTCTGATTGTGGGTGTGGATGACAAAGGTGACGGTTACAAGGTTCTGAAAGAAGGTATAGATGGACCCGTTGTAGTCACTGTTGGAAAAAAAGAAATGCAGAATGGACCGTTTGATTCAAAATTTACTGCCTTGGATCTGAACAAGAAACAAATATCAGTAAATGATGTTGTGAAGATTTCCAAAGGCCCATCCGAGGGTAAACAAGGAGTTGTAAGGCAAGTATACAGAGGGATCATATTCCTTTATGATGAGAGCGAGGAAGAAAATGGTGGATATTTCTGCTGCAAATCACAATCATGTGAGAAAGTTAAACTCTTTACTGAAGAGTCCAATGAGAAGACTGGTGGATTTGATGGTACAGCTTTTGAAGATTTTGTATCCTCCCCAAAATCGCCGCTATCTCCTGAAAAAGAGTGGCAGCCCAGGGAAAGATACAATAGCTCCAACCAAGGAGACATAGGTAGCACGTATTCTATAGGCCAAAAGCTGAGGATACGGGTGGGTCCATTGAAGGGGTATCTCTGCCGTGTAATAGCTTTACGTTATTCTGATGTTACAGTCAAGCTTGATTCCCAGCATAAAATTTTCACAGTTAAAAGTGAGCATCTTGCTGAGGTTCGCGACAGAAACACTGTGCTAAGTACAAGTGGGGATGCGGGTACTGGTTCCTTTCAACCTTTTGGCATGCTTGGAACAGAAAGCAGCACTGGAGACTGGGCGATAGGAGCAGGGACGTCAAGTGAGGGTGGTAACTGGAATATAGGAGGCCCTTCTACTGACTCGCATGAGAGTCTCAATATTGAACGTAACATGGTTCAACTGTGCCGTGAGAAGAACCCATGGGGCGGCAGTAAGCCTACGTCTGACGTCTCGCCAACAGTTGCAGATGATAATACTTCAGCATGGGCCAATGCAGCAGCTGAGAACAAACCTGCCTCGGCTAGTGATCAACCTGGTGGTTGGAATCCCTGGGGTAAAACACCTGCTTCTGAAGCTGGCACTGTTAGTGGATGGGGAGATACAAGTGCTTCAAATGTTGAAGCTTCTTCGTGGGAAAAACAAGGAGCTTCTACTTCCAATGTTGCAGATTTAGGTTCCTGGGGTACGCATGGTGGAAGCTCTGGTGGTAATAAGCAAGATGAGGACTCGGTATGGGGTAAACTATGTGAAGCTTCTGAATCAAGTCAGAAGAAAGAAGAATCGTCTTGGGGTAAGAAAGGGGGATCAGATGGAGAATCTAGTTGGGGAAACAAAGATGGAAATTCCAGCGCAAGTAAGAAAGATGGAGTTTCCTGGGGACAACAGGATAAAGGCTCTGACGAGAGCAAGGGAGGATCTGCATGGAGTAATCAATGTGGAGATTTTGGTTCAGGGAAGAAAAAAGATGGTTCTTCTGGTTGGAACAAGTCAGCAGAAGATTCCAATGCCAATAGTAAGGGAGTTCCTGACTGGGGCCAACCAAATGATGGCTCTTCATGGGGTAAAAAGGGTGACGGAGCGGCATCGTGGGGTAAAAAGGATGACGGAGGCTCGTGGGGTAAAAAGGATGACGGTAATAAGGATGATGGAGGATCATCGTGGGGTAAGAAAGATGACGGTCAAAAGGATGACGGAGGATCGTCTTGGGAGAAAAAGTTTGATGGAGGATCATCATGGGGTAAGAAGGACGATGGAGGATCTTCTTGGGGCAAAAAGGATGATGGAGGATCGTTGTGGGGAAAAAAGGATGATGGAGGATCTTCTTGGGGCAAAGAGGATGATGGAGGATCGTTGTGGGGTAAGAAGGATGATGGAGAATCGTCGTGGGGTAAGAAGGATGATGGAGAATCGTCGTGGGGTAAGAAGGATGATGGAGGATCGTCGTGGGGTAAGAAGGATGAGGGAGGGTACTCAGAACAAACATTTGATAGGGGAGGACGTGGGTTTGGTGGGAGAAGAGGAGGAGGTCGTCGAGGTGGCAGGGATCAGTTTGGGAGGGGAAGCTCCTTCGGTAATTCTGAGGATCCGGCTCCATGGAGTAAACCAAGTGGCGGATCTAGCTGGGGCAAGCAAGATGGCGATGGGGGCGGTTCAAGCTGGGGTAAAGAGAATGATGCTGGTGGTGGATCTAGCTGGGGAAAGCAAGACAATGGTGTTGGTTCAAGCTGGGGTAAACAGAACGATGGTAGTGGTGGTGGATCTAGCTGGGGCAAGCAGAATGATGCTGGTGGGGGATCTAGCTGGGGCAAGCAAGACAGTGGTGGGGATGGTTCGAGCTGGGGTAAGCAGGATGGTGGTGGTGATAGTGGTTCTGCTTGGGGTAAGCAGAACAATACTAGTGGTGGATCGAGCTGGGGAAAGCAGAGTGATGCTGGTGGGGGATCTAGCTGGGGCAAGCAAGACGGTGGTGGGGGTGGTTCAAGCTGGGGTAAACAAGATGGTGGTGGAGGCAGTGGTTCTGCCTGGGGTAAGCAGAACGAGACTAGCAATGGATCGAGCTGGGGGAAGCAGAATGATTCTGGTGGAGGATCAAGCTGGGGAAAGCAAGATGGTGGTGGGGGTGGTTCAAGCTGGGGTAAACAGAACGATGGTGGTGGTGGGTCGAGCTGGGGCAAGCAGGGTGATGGTGGTTCTAAACCTTGGAATGAACATAGTGGTGGTGGTCGTGGGTTTGGTGAAAGAAGGGGAGGAGGGGGATTCCGTGGAGGTAGAAACCAATCAGGCAGAGGAGGAAGGTCGTTTGATGGTGGTCGATCATCTAGCTGGAAAACAGACAATCAAGAAAACACTTGGAAGAGCGACCAAAGTGGTGGATCAGACTGGAAAAAAGGTTGGGGAGAGGATTCAAACAATAGCAAACCATCCGGTTCGTCTGCTGGTGGTTGTGCTGGAAACTGGCCTAGCTGGGATACTAACTCAAAGAAAGAAACCAATGACAAACCGGGAGACGATAGCAAATCAGCTTGGGGAACCAGTAACGATCAGGTGAATACCGATAACAACAATGACAGTTGGAACAAAAAGCCAAACAATGATGTTGGAACAAGTGGAGAAGCTGATAATGCATGGGGCGGCAAAACAAATGCAGTAGCGCCTTCACCAAGTGGCTCGGCAGCGTGGGGAACCGGTGACAAAAAAACCGGATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1493

Amino Acids

158.04

Weight (kDa)

5.53

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 148 - 231 8.6e-20 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 240 - 365 9e-29 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 435 - 485 5.7e-06 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 494 - 548 2.2e-22 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 610 - 654 5.8e-06 Transcription elongation factor SPT5, fifth KOW domain
RDM3_C PF23348 972 - 1030 1e-07 RDM3 beta-solenoid
RDM3_C PF23348 1027 - 1141 5e-12 RDM3 beta-solenoid
RDM3_C PF23348 1138 - 1319 1e-30 RDM3 beta-solenoid
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2344
AatII GACGTC 2 cut(s) 2090, 2223
Acc36I ACCTGC 3 cut(s) 1190, 2293, 2344
AccB1I GGYRCC 1 cut(s) 527
AccB7I CCANNNNNTGG 2 cut(s) 359, 2819
AccBSI CCGCTC 1 cut(s) 2852
AccI GTMKAC 2 cut(s) 1104, 1575
AccII CGCG 1 cut(s) 1974
AciI CCGC 9 cut(s) 54, 593, 1748, 2009, 2199, 2852, 3384, 3420, 4404
AcsI RAATTY 8 cut(s) 80, 320, 503, 1091, 1143, 1472, 1935, 2614
AcuI CTGAAG 5 cut(s) 116, 174, 1695, 2235, 2364
AcyI GRCGYC 2 cut(s) 2087, 2220
AdeI CACNNNGTG 1 cut(s) 4436
AfaI GTAC 5 cut(s) 1711, 1996, 2014, 2458, 3242
AfiI CCNNNNNNNGG 8 cut(s) 359, 1546, 1547, 1865, 2198, 2804, 2819, 3383
AgeI ACCGGT 1 cut(s) 4457
AhlI ACTAGT 1 cut(s) 3668
AjiI CACGTC 1 cut(s) 3272
AjnI CCWGG 8 cut(s) 352, 1055, 1775, 2305, 2321, 2450, 2645, 3794
Alw21I GWGCWC 1 cut(s) 927
Alw26I GTCTC 7 cut(s) 497, 1800, 2058, 2150, 2227, 3806, 4272
AlwNI CAGNNNCTG 2 cut(s) 359, 2273
Ama87I CYCGRG 1 cut(s) 926
AoxI GGCC 8 cut(s) 843, 886, 1537, 1831, 2119, 2259, 2806, 4229
ApeKI GCWGC 7 cut(s) 174, 1635, 1771, 2055, 2267, 2270, 4445
ApoI RAATTY 8 cut(s) 80, 320, 503, 1091, 1143, 1472, 1935, 2614
AsiGI ACCGGT 1 cut(s) 4457
AspLEI GCGC 3 cut(s) 114, 2624, 4426
AspS9I GGNCC 8 cut(s) 562, 1415, 1457, 1538, 1856, 2120, 2259, 2806
AsuC2I CCSGG 1 cut(s) 4275
AsuII TTCGAA 1 cut(s) 141
AvaI CYCGRG 1 cut(s) 926
AvaII GGWCC 4 cut(s) 562, 1415, 1457, 1856
AxyI CCTNAGG 2 cut(s) 123, 231
BamHI GGATCC 2 cut(s) 433, 3355
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 2 cut(s) 739, 927
BauI CACGAG 2 cut(s) 738, 2884
BbsI GAAGAC 2 cut(s) 228, 1697
Bbv12I GWGCWC 1 cut(s) 927
BbvCI CCTCAGC 2 cut(s) 1841, 1965
BbvI GCAGC 7 cut(s) 186, 1622, 1783, 2067, 2279, 2282, 4457
BceAI ACGGC 2 cut(s) 1863, 2163
BciT130I CCWGG 8 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796
BciVI GTATCC 4 cut(s) 1045, 1741, 1839, 4234
BclI TGATCA 2 cut(s) 909, 2299
BcnI CCSGG 1 cut(s) 4275
BcoDI GTCTC 7 cut(s) 497, 1800, 2058, 2150, 2227, 3806, 4272
BcuI ACTAGT 1 cut(s) 3668
BfmI CTRYAG 3 cut(s) 1101, 1183, 1827
BfoI RGCGCY 1 cut(s) 4427
BfuAI ACCTGC 3 cut(s) 1190, 2293, 2344
BfuI GTATCC 4 cut(s) 1045, 1741, 1839, 4234
BglII AGATCT 1 cut(s) 766
Bme1390I CCNGG 9 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796, 4275
Bme18I GGWCC 4 cut(s) 562, 1415, 1457, 1856
BmeT110I CYCGRG 1 cut(s) 926
BmgBI CACGTC 1 cut(s) 3272
BmgT120I GGNCC 8 cut(s) 562, 1415, 1457, 1538, 1856, 2120, 2259, 2806
BmrFI CCNGG 9 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796, 4275
BmrI ACTGGG 2 cut(s) 2077, 2812
BmsI GCATC 9 cut(s) 385, 400, 993, 1966, 1996, 2864, 3436, 3544, 3691
BmuI ACTGGG 2 cut(s) 2077, 2812
BoxI GACNNNNGTC 1 cut(s) 1350
BpiI GAAGAC 2 cut(s) 228, 1697
BplI GAGNNNNNCTC 4 cut(s) 781, 813, 1121, 1153
BpmI CTGGAG 2 cut(s) 879, 2082
Bpu10I CCTNAGC 2 cut(s) 1841, 1965
Bpu14I TTCGAA 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 1 cut(s) 4275
BsaAI YACGTR 1 cut(s) 1821
BsaBI GATNNNNATC 1 cut(s) 1017
BsaHI GRCGYC 2 cut(s) 2087, 2220
BsaWI WCCGGW 3 cut(s) 4196, 4457, 4472
BsaXI ACNNNNNCTCC 8 cut(s) 861, 883, 891, 913, 1778, 1808, 2972, 3002
Bsc4I CCNNNNNNNGG 8 cut(s) 359, 1546, 1547, 1865, 2198, 2804, 2819, 3383
Bse118I RCCGGY 2 cut(s) 32, 4457
Bse21I CCTNAGG 2 cut(s) 123, 231
Bse3DI GCAATG 1 cut(s) 3826
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 8 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 8 cut(s) 359, 1546, 1547, 1865, 2198, 2804, 2819, 3383
BseMI GCAATG 1 cut(s) 3826
BseRI GAGGAG 6 cut(s) 329, 944, 3306, 3309, 4035, 4074
BseXI GCAGC 7 cut(s) 186, 1622, 1783, 2067, 2279, 2282, 4457
Bsh1236I CGCG 1 cut(s) 1974
BshFI GGCC 8 cut(s) 845, 888, 1539, 1833, 2121, 2261, 2808, 4231
BshNI GGYRCC 1 cut(s) 527
BshTI ACCGGT 1 cut(s) 4457
BsiHKAI GWGCWC 1 cut(s) 927
BsiHKCI CYCGRG 1 cut(s) 926
BsiSI CCGG 7 cut(s) 33, 51, 3359, 4197, 4274, 4458, 4473
BslFI GGGAC 2 cut(s) 2098, 2664
BslI CCNNNNNNNGG 8 cut(s) 359, 1546, 1547, 1865, 2198, 2804, 2819, 3383
BsmAI GTCTC 7 cut(s) 497, 1800, 2058, 2150, 2227, 3806, 4272
BsmBI CGTCTC 2 cut(s) 2227, 4272
BsmFI GGGAC 2 cut(s) 2098, 2664
BsnI GGCC 8 cut(s) 845, 888, 1539, 1833, 2121, 2261, 2808, 4231
BsoBI CYCGRG 1 cut(s) 926
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 739, 927
Bsp19I CCATGG 2 cut(s) 2192, 3365
Bsp68I TCGCGA 1 cut(s) 1974
BspACI CCGC 9 cut(s) 54, 593, 1748, 2009, 2199, 2852, 3384, 3420, 4404
BspANI GGCC 8 cut(s) 845, 888, 1539, 1833, 2121, 2261, 2808, 4231
BspFNI CGCG 1 cut(s) 1974
BspMAI CTGCAG 1 cut(s) 1187
BspMI ACCTGC 3 cut(s) 1190, 2293, 2344
BspQI GCTCTTC 2 cut(s) 2830, 4121
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 527
BsrBI CCGCTC 1 cut(s) 2852
BsrDI GCAATG 1 cut(s) 3826
BsrFI RCCGGY 2 cut(s) 32, 4457
BssAI RCCGGY 2 cut(s) 32, 4457
BssNAI GTATAC 1 cut(s) 1576
BssNI GRCGYC 2 cut(s) 2087, 2220
BssSI CACGAG 2 cut(s) 738, 2884
BssT1I CCWWGG 7 cut(s) 294, 429, 1482, 1801, 2192, 3365, 3971
Bst1107I GTATAC 1 cut(s) 1576
Bst2BI CACGAG 2 cut(s) 738, 2884
Bst2UI CCWGG 8 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796
Bst6I CTCTTC 5 cut(s) 234, 1671, 2830, 3283, 4121
BstACI GRCGYC 2 cut(s) 2087, 2220
BstBAI YACGTR 1 cut(s) 1821
BstBI TTCGAA 1 cut(s) 141
BstDSI CCRYGG 3 cut(s) 2192, 3365, 4033
BstFNI CGCG 1 cut(s) 1974
BstH2I RGCGCY 1 cut(s) 4427
BstHHI GCGC 3 cut(s) 114, 2624, 4426
BstMAI GTCTC 7 cut(s) 497, 1800, 2058, 2150, 2227, 3806, 4272
BstMWI GCNNNNNNNGC 7 cut(s) 2078, 2267, 2345, 3390, 3417, 3426, 4394
BstNI CCWGG 8 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796
BstNSI RCATGY 2 cut(s) 624, 2041
BstPAI GACNNNNGTC 1 cut(s) 1350
BstSCI CCNGG 9 cut(s) 352, 1055, 1775, 2305, 2321, 2450, 2645, 3794, 4273
BstSFI CTRYAG 3 cut(s) 1101, 1183, 1827
BstUI CGCG 1 cut(s) 1974
BstV1I GCAGC 7 cut(s) 186, 1622, 1783, 2067, 2279, 2282, 4457
BstV2I GAAGAC 2 cut(s) 228, 1697
BstXI CCANNNNNNTGG 3 cut(s) 406, 860, 4142
BstZ17I GTATAC 1 cut(s) 1576
Bsu36I CCTNAGG 2 cut(s) 123, 231
BsuI GTATCC 4 cut(s) 1045, 1741, 1839, 4234
BsuRI GGCC 8 cut(s) 845, 888, 1539, 1833, 2121, 2261, 2808, 4231
BtgI CCRYGG 3 cut(s) 2192, 3365, 4033
BtgZI GCGATG 1 cut(s) 3426
BtrI CACGTC 1 cut(s) 3272
BtsI GCAGTG 1 cut(s) 3790
BtsIMutI CAGTG 6 cut(s) 1428, 1983, 2058, 2352, 3595, 3790
BtuMI TCGCGA 1 cut(s) 1974
BveI ACCTGC 3 cut(s) 1190, 2293, 2344
CaiI CAGNNNCTG 2 cut(s) 359, 2273
CfoI GCGC 3 cut(s) 114, 2624, 4426
Cfr10I RCCGGY 2 cut(s) 32, 4457
Cfr13I GGNCC 8 cut(s) 562, 1415, 1457, 1538, 1856, 2120, 2259, 2806
CsiI ACCWGGT 1 cut(s) 2305
Csp6I GTAC 5 cut(s) 1710, 1995, 2013, 2457, 3241
CspAI ACCGGT 1 cut(s) 4457
CspCI CAANNNNNGTGG 2 cut(s) 3652, 3687
CviQI GTAC 5 cut(s) 1710, 1995, 2013, 2457, 3241
DraI TTTAAA 1 cut(s) 309
DraIII CACNNNGTG 1 cut(s) 4436
Eam1104I CTCTTC 5 cut(s) 234, 1671, 2830, 3283, 4121
EarI CTCTTC 5 cut(s) 234, 1671, 2830, 3283, 4121
EciI GGCGGA 2 cut(s) 69, 3399
Ecl136II GAGCTC 1 cut(s) 925
Eco130I CCWWGG 7 cut(s) 294, 429, 1482, 1801, 2192, 3365, 3971
Eco147I AGGCCT 1 cut(s) 845
Eco24I GRGCYC 2 cut(s) 739, 927
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 4 cut(s) 562, 1415, 1457, 1856
Eco53kI GAGCTC 1 cut(s) 925
Eco57I CTGAAG 5 cut(s) 116, 174, 1695, 2235, 2364
Eco81I CCTNAGG 2 cut(s) 123, 231
Eco88I CYCGRG 1 cut(s) 926
EcoICRI GAGCTC 1 cut(s) 925
EcoO109I RGGNCCY 1 cut(s) 2120
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 8 cut(s) 352, 1055, 1775, 2305, 2321, 2450, 2645, 3794
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 7 cut(s) 294, 429, 1482, 1801, 2192, 3365, 3971
EcoT22I ATGCAT 1 cut(s) 4399
EcoT38I GRGCYC 2 cut(s) 739, 927
ErhI CCWWGG 7 cut(s) 294, 429, 1482, 1801, 2192, 3365, 3971
Esp3I CGTCTC 2 cut(s) 2227, 4272
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FaqI GGGAC 2 cut(s) 2098, 2664
FauI CCCGC 1 cut(s) 2002
FbaI TGATCA 2 cut(s) 909, 2299
FblI GTMKAC 2 cut(s) 1104, 1575
FriOI GRGCYC 2 cut(s) 739, 927
GlaI GCGC 3 cut(s) 113, 2623, 4425
GsuI CTGGAG 2 cut(s) 879, 2082
HaeII RGCGCY 1 cut(s) 4427
HaeIII GGCC 8 cut(s) 845, 888, 1539, 1833, 2121, 2261, 2808, 4231
HapII CCGG 7 cut(s) 33, 51, 3359, 4197, 4274, 4458, 4473
HhaI GCGC 3 cut(s) 114, 2624, 4426
Hin1I GRCGYC 2 cut(s) 2087, 2220
Hin6I GCGC 3 cut(s) 112, 2622, 4424
HinP1I GCGC 3 cut(s) 112, 2622, 4424
HindIII AAGCTT 3 cut(s) 1916, 2394, 2523
HpaII CCGG 7 cut(s) 33, 51, 3359, 4197, 4274, 4458, 4473
Hpy188III TCNNGA 7 cut(s) 614, 965, 992, 1757, 1973, 2798, 4112
Hpy99I CGWCG 6 cut(s) 80, 185, 3157, 3187, 3217, 3306
HpyCH4IV ACGT 7 cut(s) 1820, 1894, 2087, 2158, 2213, 2220, 3271
HpyF10VI GCNNNNNNNGC 7 cut(s) 2078, 2267, 2345, 3390, 3417, 3426, 4394
HpySE526I ACGT 7 cut(s) 1820, 1894, 2087, 2158, 2213, 2220, 3271
Hsp92I GRCGYC 2 cut(s) 2087, 2220
HspAI GCGC 3 cut(s) 112, 2622, 4424
Ksp22I TGATCA 2 cut(s) 909, 2299
LguI GCTCTTC 2 cut(s) 2830, 4121
LmnI GCTCC 9 cut(s) 182, 424, 904, 1799, 2078, 2417, 2849, 3341, 3367
Lsp1109I GCAGC 7 cut(s) 186, 1622, 1783, 2067, 2279, 2282, 4457
LweI GCATC 9 cut(s) 385, 400, 993, 1966, 1996, 2864, 3436, 3544, 3691
MabI ACCWGGT 1 cut(s) 2305
MaeII ACGT 7 cut(s) 1820, 1894, 2087, 2158, 2213, 2220, 3271
MbiI CCGCTC 1 cut(s) 2852
MhlI GDGCHC 2 cut(s) 739, 927
MlyI GAGTC 5 cut(s) 886, 1688, 2126, 2152, 2492
MmeI TCCRAC 9 cut(s) 204, 446, 1202, 1414, 1821, 2294, 2732, 4325, 4351
Mph1103I ATGCAT 1 cut(s) 4399
MseI TTAA 6 cut(s) 105, 210, 308, 744, 1662, 1947
MslI CAYNNNNRTG 4 cut(s) 404, 950, 1648, 3987
MspA1I CMGCKG 2 cut(s) 152, 2273
MspI CCGG 7 cut(s) 33, 51, 3359, 4197, 4274, 4458, 4473
MspR9I CCNGG 9 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796, 4275
MvaI CCWGG 8 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796
MvnI CGCG 1 cut(s) 1974
MwoI GCNNNNNNNGC 7 cut(s) 2078, 2267, 2345, 3390, 3417, 3426, 4394
NciI CCSGG 1 cut(s) 4275
NcoI CCATGG 2 cut(s) 2192, 3365
NmeAIII GCCGAG 2 cut(s) 2270, 4421
NmuCI GTSAC 5 cut(s) 791, 1379, 1426, 2843, 4460
NruI TCGCGA 1 cut(s) 1974
NsiI ATGCAT 1 cut(s) 4399
NspI RCATGY 2 cut(s) 624, 2041
NspV TTCGAA 1 cut(s) 141
PaeI GCATGC 2 cut(s) 624, 2041
PaeR7I CTCGAG 1 cut(s) 926
PaqCI CACCTGC 1 cut(s) 2344
PasI CCCWGGG 2 cut(s) 1776, 2322
PceI AGGCCT 1 cut(s) 845
PciSI GCTCTTC 2 cut(s) 2830, 4121
PcsI WCGNNNNNNNCGW 1 cut(s) 475
PflFI GACNNNGTC 1 cut(s) 566
PflMI CCANNNNNTGG 2 cut(s) 359, 2819
PinAI ACCGGT 1 cut(s) 4457
PleI GAGTC 5 cut(s) 886, 1687, 2126, 2151, 2492
PpsI GAGTC 5 cut(s) 886, 1687, 2126, 2151, 2492
Ppu21I YACGTR 1 cut(s) 1821
PshAI GACNNNNGTC 1 cut(s) 1350
Psp124BI GAGCTC 1 cut(s) 927
Psp6I CCWGG 8 cut(s) 352, 1055, 1775, 2305, 2321, 2450, 2645, 3794
PspGI CCWGG 8 cut(s) 352, 1055, 1775, 2305, 2321, 2450, 2645, 3794
PspPI GGNCC 8 cut(s) 562, 1415, 1457, 1538, 1856, 2120, 2259, 2806
PspXI VCTCGAGB 1 cut(s) 926
PstI CTGCAG 1 cut(s) 1187
PstNI CAGNNNCTG 2 cut(s) 359, 2273
PsyI GACNNNGTC 1 cut(s) 566
PvuII CAGCTG 2 cut(s) 152, 2273
RruI TCGCGA 1 cut(s) 1974
RsaI GTAC 5 cut(s) 1711, 1996, 2014, 2458, 3242
RsaNI GTAC 5 cut(s) 1710, 1995, 2013, 2457, 3241
RseI CAYNNNNRTG 4 cut(s) 404, 950, 1648, 3987
SacI GAGCTC 1 cut(s) 927
SapI GCTCTTC 2 cut(s) 2830, 4121
SaqAI TTAA 6 cut(s) 105, 210, 308, 744, 1662, 1947
Sau96I GGNCC 8 cut(s) 562, 1415, 1457, 1538, 1856, 2120, 2259, 2806
SchI GAGTC 5 cut(s) 886, 1688, 2126, 2152, 2492
ScrFI CCNGG 9 cut(s) 354, 1057, 1777, 2307, 2323, 2452, 2647, 3796, 4275
SduI GDGCHC 2 cut(s) 739, 927
SexAI ACCWGGT 1 cut(s) 2305
SfaNI GCATC 9 cut(s) 385, 400, 993, 1966, 1996, 2864, 3436, 3544, 3691
SfcI CTRYAG 3 cut(s) 1101, 1183, 1827
Sfr274I CTCGAG 1 cut(s) 926
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 4 cut(s) 562, 1415, 1457, 1856
SlaI CTCGAG 1 cut(s) 926
SmiMI CAYNNNNRTG 4 cut(s) 404, 950, 1648, 3987
SmlI CTYRAG 2 cut(s) 614, 926
SmoI CTYRAG 2 cut(s) 614, 926
SpeI ACTAGT 1 cut(s) 3668
SphI GCATGC 2 cut(s) 624, 2041
SseBI AGGCCT 1 cut(s) 845
SsiI CCGC 9 cut(s) 54, 593, 1748, 2009, 2199, 2852, 3384, 3420, 4404
SspI AATATT 1 cut(s) 2152
SstI GAGCTC 1 cut(s) 927
StuI AGGCCT 1 cut(s) 845
StyD4I CCNGG 9 cut(s) 352, 1055, 1775, 2305, 2321, 2450, 2645, 3794, 4273
StyI CCWWGG 7 cut(s) 294, 429, 1482, 1801, 2192, 3365, 3971
TaiI ACGT 7 cut(s) 1823, 1897, 2090, 2161, 2216, 2223, 3274
TaqII GACCGA 1 cut(s) 1122
TatI WGTACW 1 cut(s) 1994
TauI GCSGC 4 cut(s) 1750, 2202, 2855, 4407
Tru1I TTAA 6 cut(s) 105, 210, 308, 744, 1662, 1947
Tru9I TTAA 6 cut(s) 105, 210, 308, 744, 1662, 1947
TscAI CASTG 6 cut(s) 1435, 1990, 2065, 2359, 3595, 3790
TseFI GTSAC 5 cut(s) 791, 1379, 1426, 2843, 4460
TseI GCWGC 7 cut(s) 174, 1635, 1771, 2055, 2267, 2270, 4445
Tsp45I GTSAC 5 cut(s) 791, 1379, 1426, 2843, 4460
TspDTI ATGAA 6 cut(s) 105, 568, 574, 1272, 2817, 3993
TspGWI ACGGA 8 cut(s) 64, 183, 210, 472, 2862, 2892, 2979, 4022
TspRI CASTG 6 cut(s) 1435, 1990, 2065, 2359, 3595, 3790
Tth111I GACNNNGTC 1 cut(s) 566
Van91I CCANNNNNTGG 2 cut(s) 359, 2819
VpaK11BI GGWCC 4 cut(s) 562, 1415, 1457, 1856
XapI RAATTY 8 cut(s) 80, 320, 503, 1091, 1143, 1472, 1935, 2614
XceI RCATGY 2 cut(s) 624, 2041
XcmI CCANNNNNNNNNTGG 2 cut(s) 860, 2816
XhoI CTCGAG 1 cut(s) 926
XmiI GTMKAC 2 cut(s) 1104, 1575
ZraI GACGTC 2 cut(s) 2088, 2221
Zsp2I ATGCAT 1 cut(s) 4399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.