Prupe.2G206300_v2.0.a1

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0012966
Physical Location & Seq
Reverse (-)
24061053 .. 24070973
9921 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G206300.3

Sequence Viewer

Length: 5154 bp
ATGACTTCGAAGGGCAAAGGGATCGCCGGAGGTGGATCCTCCGGCGGCAAGCGTAAGTACGACGATGACAAGACCGGCGGTGGCCGGAAGAGGATCGACCGCGGCGTCCTCAAGTTCTTCGAAGATGTAGCTGCGGAAGCCGACGGCGGCGACGACAGTGACTTCGATGACGATTTTATGGAAGAAGAATTTGAAACAGAACCTATAGTCAAGAATGAACCAGGGAAAGCACATAACCTTCCATTCATTCCTAAAGAGGAGGATATGGATGGTGAAGAATTTGAAAAAATGATGGAAGAACGATACAGGAGTGGTTCTAGCTATATTACCTATGCCGAAGATAATTATGAGAACAAAAGGTCGGTTGACGGCAGTGTTCTTCTGCCTACTGTTAAGGATCCAATCATCTGGAAAGTGAAATGCATGGTTGGACGCGAGAGGCATTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTACGATCGCTAGGTACCAAACTGGAGATAATTTCTGCGTTTGCTGTTGAGCACATCAAGGGTTTTGTTTTCATTGAAGCTGACAAACAATCTGATATTAATGAGGCATGCAAAGGAATTTGTAGCATATATTCGTCCCGAGTAATGCCAGTTCCAAATAATGAAGTTTCTCATTTGCTGTCTCCTCGGACTAGATACAATGGAATTACTGTAGGCATGTGGGCCCGCGTGAAGAGTGGGAACTACAAGGGTGATTTGGCACAGGTTGTGTTCGTAAACGATTTACGAAAACGAGCGACAGTAAAGCTGATCCCAAGAATCAATCTACAAGCAATGGCTGCAAAATTTGGTGGGGGAGGTACTAGAAAGAAAGTCCCCGCTCCTGCACCAAGATTGATCAACTCAAGCGAACTCGAAGAGTTCCGCCCTCTCATTCAGTGCAGGAATGATCGTGAAAGTGGCATGAGGTTTGAATTTCTTGATGGTCTGATGTTCAAGGATGGATATTTATACAAAAAAGTACCCATAGATTCATTAAGCTTCTGGGGTGTTATGCCATCAGAAGAGGAGCTACTGAAATTCAAGTCTTCTGAGAACAATGAAAGTGATAATTTGGAGTGGCTGACAGAGCTTTATGGTAAGGAAAAGAAAAGACGGACCATCAAAATTGAGGAAGGGGGTGGGAAAGGAGAGGGCTCATCTGGTTGTATGGGGAAAGGAGAGGGCTCATCTGGGTCTATGGGGAAAGGAGAGGGCTCATCGGGTTCTATGGGAAAAGGAGAGGGCGCATCGGGTTCTATGGGTAAAGGAGAGGGCGCATCAGGTTCTATGGGTAAGGGAGAGGGATCATCAGGTTCTATGGGGAAAGGAGAGGGTTCATCAGGGTCTGTGGGGAAAGGAGTGGGTTCATCAGGATCTGGTGGGAATTGCTTTGAACTTTATGATCTTGTTTGTCTTGGTCGGAAAGATTTTGGTCTTGTAATAGGCATGGAGAAAGATGATAGTTACAAGATTCTGAAAGAGGGGCTGGAAGGACCTGTTGTTCTGATTGTTCAGAAACGTGAGCTAAAGAATGTTCTTTCTGATATGAAATTTACGGCTTTAGATCGGCGCACGAAGCCCATATGCGTTAGTGATACTGTCAAAGTGTTGGAAGGACCATTGAAGGATAGGCAGGGGATTGTTAGGCAAATCTATAGAGGTACCATATTCTTGTATGATGAGAACGAGACAGAAAATGGTGGTTATTTCTGCTCAAAATCTCATATGTGTGAGAAAATTAAGCTTTATAATGATGCTTGCAAAGAAAAGGATGGGGATTCAGGTGGTCCAGTTTTTGAAGATTTCATGTCATCTCCTAAATCCCCTCTATCGCCAAAAAAGCCATGGCAAGAAAGAGATAGTAACTTTAATCGGGGTGATACAGATGGAATTTTCTCAATTGGTCAAACTGTAAGAATCCGTGTGGGTCCCTTGAAGGGATACCTCTGTCGCATTTTGGCTATACGCCGTGCTGATATCACTGTTAAGCTTGATTCTCAGCAGAAGGTTCTTACAGTTAAATGTGAGCATCTTTCTGAAGTTCGTGGAAAGAGCTCGTCTGTGCTGATTAGTGAGGATTCGGAGTCCGGTTTAAAACCATTTGATATGCTTGGAAATGAAGGAGGCTCTAAAGATTGGACAGATGGAGCTGGGGCATCAGCTGGCGGTGCTGGCGATGGGTGGAATGCTGGAGGGGCATCTGGTGAAAGGAATTCTTGGCCTAGTTTCTCTGCCACAGGCATTTCGATTCAGTCAGAGTCCATTTCTGGTCAGGATGGAAATGATGTTAAGAAAGATGATTCTTGGGAGAGCAAAGTTGCTCCAAATAAAATTTCGTCCTGGGGTGCAGCAACAGATAACAATGATCAAGGTGCTGGCTGGGGAAAAGGTGTGGACTCTTGGGGTAAATCTAGTGCTAAAACTGGGGGTGACAGCAGTGCATCGGATATTTGGCAGAAGGCAATAGAACCTTCTGGTACTGCTACTGCTGGGAATAGCCAACTGGATTCATGGGGCAAAGGTAAAAATTTAGTTGAAGCAGGATCATGGGAAAAGAATTCTGATGCAGCATCTGGTGATATTGCTAGTTCTGGGTGGGGTCAACAGAAACCTTTGGACAAAGGAAATGCAGTCAGTAGTGGTGGGTCTGATTGGGGTAAGCCTCAGAATAAAGGAGCCGGTTGGGGAGCTAAAGAAGATTCTTGCAGTAAAGCCACAGGGAACTGGAGCACTAAAGATGAATTAAGTGCTGGTGAGGCAGGGTGGAAAATTTCAAAGCCGGCAGAAGATGTTCAGACAGGAAGCTGGGGTAATGCAGGTGGGGTTTTGCCCCAATCAGAGGCAGGAAACAAAGATGAAGCAAGTGGCTGGGCAAAACCTAAAGGCGCTTTTAGTAATGAGAATCAGAACGACAGTTGGAAGAAACCAAGTGGTGTAGATGACAATAAAAGAGCTTCCTGGGGTAAAGCAGATGGAGGTTCTGCATGGACTAAACAAGACGGAGATTCTACATGGAATAAACAAGGTGGAGGTTCTACTTGGAATAAGCAAGATGGCGGTTCTGCTTGGAATAAACCAGCAGGAGATTCTTCTTGGAGTAAACAAGCTGGAGGGTCTTCATGGGGTAAACAAGCTGATGTAACAGCAGGACATGAGTCTGACAGAGTGGGGAATCAAGATGATGGTTGGAAGAGAGCAAAAAGTTTTGGCGGTGATCAAGGATCTGGTGGTTGGGGCAAAGGGAGTGGGGATAAGGGTGATATAGATCAGCAAGACTTCTCGGGAAGGCCAAAATCCTTTGAGGGGGCTCATGGGTTTGGTGGAAGAAGAGGAGGAAGAGGAGGTAGAGATCAATTTGGCAGGGGTAGGTCATTCAGTCAGGATCAATCCTCTGGGTGGAACAAGGATCGGGAAAACAATAGGAGTGCTGATGGAATAGGTGGTTGGAAGAATCCAAATGCTTCTGTTGAAAACAATGGATCGGGTTGGAGCAAAGGATGGGGGGCAGAGAAAGAGAATGTTGAAGAACAATCAACAGGGGGTAACAAATCTGGTGATTGGAATGCTCCAAAATCTTCTGACAAAGACCAAACGTCAGGATGGGGTCAAACTAAAGCTTGGCAAAGTGGGTCAAGTGATGGAGGAAATCAAGTTTCTAGCTGGGGCCAAAAGGGCAGTTGGAACTCCAGATCTAGTGAGGCTGGTGGCAATCAGGATTCTAGTAGTGGTGGGAAAAGAGATTGGAACATTGGATCTGATTCCTCAGGTGGGAACCAGGATTCTACTTGGGGCAAGAAAAGCAACTGGAACTCCGGATCTGGTGACACTGGCGGCAATAAAGATTCTGGCTGGGGAAGGAAAAATAGTTGGAACTCAGGATCTGGTGAGGCAGATCAGAATTCTAATTGGAGCAGTAAAAGCAATTGGAATTCAGCAAATTCTTTTGGTGGCAGTCAATCAATAGATGGTGGGAATGGAGACCAACCTGAAGATTTCAATAATAATCGAAGTGGTGGAAATTGGAGAGGTGGTTCAGGTAGAGGAAATTCAGACAGAGGAGGTTTTAGAGGTGGAGGCGGTGAGAGAGAGGGAGATAGAGGGGGTTTTGGACGTAGAGGGGGTTTTGGTGGTAGAGGTGGAGACAGAGGGAGCTTTGGTGGTAGGGGTAGATCAGACAGAGGAGGGTTTGGTGGTAGAGGAGGATCAGACGGAGGGGGGTTTGGTGGTACAGGTGGATCAGACGGAGGAGGGTTTGGTGGTACAGGCTATGGAGGAAGAGGCCGTGGGAGGGATCAAAGTGGTGGTTGGAGCAATAGAAATGATTCTTTTGATAATAATTCATCAGGCTGGAGCAAAGGGGCAGACGGTGCTGGCGAGGGATGGAAAAAAGACAATGGTGGAGGAAGTTGGAACCAAGGAGGTGGCAGTAAGAACGATTGGCAGGGTGGGAAATCTAGCGGATGGAGTAGCCAGAGCTCTGGTTGGAACCAGTCTGATGTGAATAAAGGAATTGGCGGTTCGGGATCAGGTTGGAATCAGACAGTGGAGGCTAAAGATACTGCTGCCACTCAGGACAAAGGAACAGGCTCTCGTAATGAAGCGAGCGGAAGCTGGGGAAACAATTGGAAATCATCAGATGCTTCTAATGTGGACCAGTCGTCTAGCTGGAAACAATCGACTGCAGCCAAAGAAATCAAAGGAACCACTGATCAAGATGGTGGCCAGAACAAGGGACCAAGCTCAAGCGCACAAGCTGGGGGCTTGGGCAACCAGGGTTCAGGTTGGAACAAAGGAACAGGTTCAGGATTTGGAGGCGGTACTGGAGACCAACCAAGTGCTGCTGGAGGAGGTAAATCATCCGACTGGAAGCAATCAAGCACTTCAAGTGGGGCTCAGTCCTGTGGTTGGAATCAATCAGGTGAGGCAAAACAAGGAACCGATCAAGGTGCAGAACCAACCAATTCATGGGGCAAAGCAGCAGCAGCATGCTCTTGGGGCAACGGATCTGATGGTGGTGAGGCAAAACAAGGAACCGATGAAGGTGCAAAACCGACTAATTCCTGGGGCAAAGCTGTAGCACCCGCAAGCTCTTGGGGCAAGGGATCTGATGGTGGAAGTGGCAAAGGAGGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1718

Amino Acids

179.93

Weight (kDa)

6.35

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1767
AarI CACCTGC 1 cut(s) 2825
AasI GACNNNNNNGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 2825
Acc65I GGTACC 2 cut(s) 491, 1679
AccB1I GGYRCC 2 cut(s) 491, 1679
AccB7I CCANNNNNTGG 1 cut(s) 4983
AccBSI CCGCTC 2 cut(s) 857, 4623
AccII CGCG 3 cut(s) 102, 435, 705
AccIII TCCGGA 1 cut(s) 3830
AcoI YGGCCR 2 cut(s) 82, 4738
AcuI CTGAAG 2 cut(s) 2076, 4026
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 9 cut(s) 59, 493, 838, 999, 1681, 2497, 4246, 4279, 4837
AfiI CCNNNNNNNGG 9 cut(s) 1955, 2857, 3321, 3414, 3786, 4306, 4747, 4923, 4983
AhdI GACNNNNNGTC 2 cut(s) 3610, 4675
AjnI CCWGG 6 cut(s) 220, 2357, 2975, 3792, 4788, 5078
AjuI GAANNNNNNNTTGG 4 cut(s) 2335, 2367, 3445, 3477
AloI GAACNNNNNNTCC 4 cut(s) 1503, 1535, 4033, 4065
Alw21I GWGCWC 4 cut(s) 531, 2075, 2750, 4496
Alw26I GTCTC 5 cut(s) 663, 1700, 3990, 4152, 4836
AlwNI CAGNNNCTG 4 cut(s) 1364, 1394, 2591, 3899
Ama87I CYCGRG 2 cut(s) 615, 3298
Aor13HI TCCGGA 1 cut(s) 3830
AoxI GGCC 7 cut(s) 82, 699, 2237, 3305, 3682, 4297, 4738
ApaI GGGCCC 1 cut(s) 703
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AseI ATTAAT 1 cut(s) 576
Asp700I GAANNNNTTC 3 cut(s) 2808, 4339, 4816
Asp718I GGTACC 2 cut(s) 491, 1679
AspLEI GCGC 5 cut(s) 1265, 1295, 1590, 2906, 4766
AsuII TTCGAA 2 cut(s) 8, 120
AvaI CYCGRG 2 cut(s) 615, 3298
AvaII GGWCC 7 cut(s) 1134, 1511, 1634, 1805, 1946, 4669, 4751
AxyI CCTNAGG 1 cut(s) 3781
BaeGI GKGCMC 1 cut(s) 703
BaeI ACNNNNGTAYC 4 cut(s) 295, 328, 1951, 1984
BalI TGGCCA 1 cut(s) 4740
BamHI GGATCC 2 cut(s) 35, 397
BanI GGYRCC 2 cut(s) 491, 1679
BanII GRGCYC 8 cut(s) 703, 1175, 1205, 1235, 2075, 3328, 4496, 4912
BarI GAAGNNNNNNTAC 2 cut(s) 1032, 1064
BbsI GAAGAC 2 cut(s) 1056, 3126
Bbv12I GWGCWC 4 cut(s) 531, 2075, 2750, 4496
BceAI ACGGC 5 cut(s) 160, 385, 1590, 1971, 4284
BcgI CGANNNNNNTGC 6 cut(s) 4, 38, 4946, 4980, 5042, 5076
BciT130I CCWGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
BciVI GTATCC 1 cut(s) 1952
BclI TGATCA 4 cut(s) 873, 2383, 3232, 4726
BcoDI GTCTC 5 cut(s) 663, 1700, 3990, 4152, 4836
BfmI CTRYAG 5 cut(s) 204, 687, 1672, 4698, 5091
BfoI RGCGCY 1 cut(s) 2907
BfuAI ACCTGC 1 cut(s) 2825
BfuI GTATCC 1 cut(s) 1952
BglI GCCNNNNNGGC 1 cut(s) 3690
BglII AGATCT 1 cut(s) 3707
Bme1390I CCNGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
Bme18I GGWCC 7 cut(s) 1134, 1511, 1634, 1805, 1946, 4669, 4751
BmeRI GACNNNNNGTC 2 cut(s) 3610, 4675
BmeT110I CYCGRG 2 cut(s) 615, 3298
BmrFI CCNGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
BmrI ACTGGG 1 cut(s) 2451
BmuI ACTGGG 1 cut(s) 2451
BoxI GACNNNNGTC 1 cut(s) 3172
BpiI GAAGAC 2 cut(s) 1056, 3126
BpmI CTGGAG 8 cut(s) 521, 2229, 2764, 3147, 3688, 4387, 4860, 4881
Bpu14I TTCGAA 2 cut(s) 8, 120
BpuEI CTTGAG 3 cut(s) 95, 865, 4744
BsaBI GATNNNNATC 1 cut(s) 3282
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 2 cut(s) 3990, 4836
BsaWI WCCGGW 2 cut(s) 2105, 3830
BsaXI ACNNNNNCTCC 6 cut(s) 3812, 3842, 4033, 4063, 4159, 4189
Bsc4I CCNNNNNNNGG 9 cut(s) 1955, 2857, 3321, 3414, 3786, 4306, 4747, 4923, 4983
Bse118I RCCGGY 3 cut(s) 74, 2696, 2797
Bse21I CCTNAGG 1 cut(s) 3781
Bse3DI GCAATG 1 cut(s) 816
Bse8I GATNNNNATC 1 cut(s) 3282
BseAI TCCGGA 1 cut(s) 3830
BseBI CCWGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
BseGI GGATG 9 cut(s) 274, 982, 1795, 2299, 3521, 3623, 4403, 4484, 4874
BseJI GATNNNNATC 1 cut(s) 3282
BseLI CCNNNNNNNGG 9 cut(s) 1955, 2857, 3321, 3414, 3786, 4306, 4747, 4923, 4983
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 7 cut(s) 1059, 2030, 2696, 3795, 3906, 4601, 4925
BseSI GKGCMC 1 cut(s) 703
BseYI CCCAGC 9 cut(s) 2168, 2397, 2507, 2823, 2886, 3678, 3867, 4629, 4772
BsgI GTGCAG 4 cut(s) 846, 937, 2385, 4986
Bsh1236I CGCG 3 cut(s) 102, 435, 705
Bsh1285I CGRYCG 2 cut(s) 100, 485
BshFI GGCC 7 cut(s) 84, 701, 2239, 3307, 3684, 4299, 4740
BshNI GGYRCC 2 cut(s) 491, 1679
BsiEI CGRYCG 2 cut(s) 100, 485
BsiHKAI GWGCWC 4 cut(s) 531, 2075, 2750, 4496
BsiHKCI CYCGRG 2 cut(s) 615, 3298
BsiSI CCGG 8 cut(s) 27, 42, 75, 85, 2106, 2697, 2798, 3831
BslFI GGGAC 4 cut(s) 598, 836, 1932, 4764
BslI CCNNNNNNNGG 9 cut(s) 1955, 2857, 3321, 3414, 3786, 4306, 4747, 4923, 4983
BsmAI GTCTC 5 cut(s) 663, 1700, 3990, 4152, 4836
BsmFI GGGAC 4 cut(s) 598, 836, 1932, 4764
BsmI GAATGC 3 cut(s) 442, 2209, 3586
BsnI GGCC 7 cut(s) 84, 701, 2239, 3307, 3684, 4299, 4740
Bso31I GGTCTC 2 cut(s) 3990, 4836
BsoBI CYCGRG 2 cut(s) 615, 3298
Bsp119I TTCGAA 2 cut(s) 8, 120
Bsp120I GGGCCC 1 cut(s) 699
Bsp13I TCCGGA 1 cut(s) 3830
Bsp19I CCATGG 1 cut(s) 1862
BspANI GGCC 7 cut(s) 84, 701, 2239, 3307, 3684, 4299, 4740
BspCNI CTCAG 7 cut(s) 1060, 2029, 2695, 3794, 3905, 4600, 4924
BspEI TCCGGA 1 cut(s) 3830
BspFNI CGCG 3 cut(s) 102, 435, 705
BspMAI CTGCAG 1 cut(s) 4702
BspMI ACCTGC 1 cut(s) 2825
BspT104I TTCGAA 2 cut(s) 8, 120
BspT107I GGYRCC 2 cut(s) 491, 1679
BspTNI GGTCTC 2 cut(s) 3990, 4836
BsrBI CCGCTC 2 cut(s) 857, 4623
BsrDI GCAATG 1 cut(s) 816
BsrFI RCCGGY 3 cut(s) 74, 2696, 2797
BssAI RCCGGY 3 cut(s) 74, 2696, 2797
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 2 cut(s) 1862, 4432
Bst2UI CCWGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
Bst6I CTCTTC 8 cut(s) 83, 704, 888, 1035, 3203, 3340, 3349, 4288
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 2 cut(s) 815, 4385
BstBI TTCGAA 2 cut(s) 8, 120
BstDEI CTNAG 7 cut(s) 1068, 2016, 2682, 3781, 3892, 4587, 4911
BstDSI CCRYGG 3 cut(s) 100, 1862, 4300
BstF5I GGATG 9 cut(s) 274, 982, 1795, 2299, 3521, 3623, 4403, 4484, 4874
BstFNI CGCG 3 cut(s) 102, 435, 705
BstH2I RGCGCY 1 cut(s) 2907
BstHHI GCGC 5 cut(s) 1265, 1295, 1590, 2906, 4766
BstMAI GTCTC 5 cut(s) 663, 1700, 3990, 4152, 4836
BstMCI CGRYCG 2 cut(s) 100, 485
BstNI CCWGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
BstNSI RCATGY 3 cut(s) 588, 697, 5007
BstPAI GACNNNNGTC 1 cut(s) 3172
BstSCI CCNGG 6 cut(s) 220, 2357, 2975, 3792, 4788, 5078
BstSFI CTRYAG 5 cut(s) 204, 687, 1672, 4698, 5091
BstSLI GKGCMC 1 cut(s) 703
BstUI CGCG 3 cut(s) 102, 435, 705
BstV2I GAAGAC 2 cut(s) 1056, 3126
BstXI CCANNNNNNTGG 3 cut(s) 408, 4439, 4496
Bsu36I CCTNAGG 1 cut(s) 3781
BsuI GTATCC 1 cut(s) 1952
BsuRI GGCC 7 cut(s) 84, 701, 2239, 3307, 3684, 4299, 4740
BtgI CCRYGG 3 cut(s) 100, 1862, 4300
BtgZI GCGATG 1 cut(s) 2208
BtsCI GGATG 9 cut(s) 274, 982, 1795, 2299, 3521, 3623, 4403, 4484, 4874
BtsI GCAGTG 2 cut(s) 379, 2461
BtsIMutI CAGTG 8 cut(s) 163, 379, 920, 1998, 2461, 3843, 4566, 4722
BveI ACCTGC 1 cut(s) 2825
CaiI CAGNNNCTG 4 cut(s) 1364, 1394, 2591, 3899
CfoI GCGC 5 cut(s) 1265, 1295, 1590, 2906, 4766
Cfr10I RCCGGY 3 cut(s) 74, 2696, 2797
Cfr42I CCGCGG 1 cut(s) 103
CseI GACGC 2 cut(s) 94, 441
Csp6I GTAC 9 cut(s) 58, 492, 837, 998, 1680, 2496, 4245, 4278, 4836
CspCI CAANNNNNGTGG 2 cut(s) 3244, 3279
CviQI GTAC 9 cut(s) 58, 492, 837, 998, 1680, 2496, 4245, 4278, 4836
DdeI CTNAG 7 cut(s) 1068, 2016, 2682, 3781, 3892, 4587, 4911
DraI TTTAAA 1 cut(s) 2112
DrdI GACNNNNNNGTC 1 cut(s) 104
DriI GACNNNNNGTC 2 cut(s) 3610, 4675
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 2 cut(s) 82, 4738
Eam1104I CTCTTC 8 cut(s) 83, 704, 888, 1035, 3203, 3340, 3349, 4288
Eam1105I GACNNNNNGTC 2 cut(s) 3610, 4675
EarI CTCTTC 8 cut(s) 83, 704, 888, 1035, 3203, 3340, 3349, 4288
EciI GGCGGA 1 cut(s) 890
Ecl136II GAGCTC 2 cut(s) 2073, 4494
Eco130I CCWWGG 2 cut(s) 1862, 4432
Eco24I GRGCYC 8 cut(s) 703, 1175, 1205, 1235, 2075, 3328, 4496, 4912
Eco31I GGTCTC 2 cut(s) 3990, 4836
Eco32I GATATC 1 cut(s) 1996
Eco47I GGWCC 7 cut(s) 1134, 1511, 1634, 1805, 1946, 4669, 4751
Eco53kI GAGCTC 2 cut(s) 2073, 4494
Eco57I CTGAAG 2 cut(s) 2076, 4026
Eco81I CCTNAGG 1 cut(s) 3781
Eco88I CYCGRG 2 cut(s) 615, 3298
EcoICRI GAGCTC 2 cut(s) 2073, 4494
EcoO109I RGGNCCY 2 cut(s) 1511, 1946
EcoRI GAATTC 4 cut(s) 2230, 2575, 3916, 3946
EcoRII CCWGG 6 cut(s) 220, 2357, 2975, 3792, 4788, 5078
EcoRV GATATC 1 cut(s) 1996
EcoT14I CCWWGG 2 cut(s) 1862, 4432
EcoT22I ATGCAT 1 cut(s) 425
EcoT38I GRGCYC 8 cut(s) 703, 1175, 1205, 1235, 2075, 3328, 4496, 4912
ErhI CCWWGG 2 cut(s) 1862, 4432
FalI AAGNNNNNCTT 2 cut(s) 2218, 2250
FaqI GGGAC 4 cut(s) 598, 836, 1932, 4764
FauI CCCGC 3 cut(s) 710, 862, 5107
FauNDI CATATG 2 cut(s) 1601, 1743
FbaI TGATCA 4 cut(s) 873, 2383, 3232, 4726
FokI GGATG 9 cut(s) 281, 989, 1802, 2306, 3528, 3630, 4410, 4491, 4861
FriOI GRGCYC 8 cut(s) 703, 1175, 1205, 1235, 2075, 3328, 4496, 4912
GlaI GCGC 5 cut(s) 1264, 1294, 1589, 2905, 4765
GsaI CCCAGC 9 cut(s) 2172, 2401, 2511, 2827, 2890, 3682, 3871, 4633, 4776
GsuI CTGGAG 8 cut(s) 521, 2229, 2764, 3147, 3688, 4387, 4860, 4881
HaeII RGCGCY 1 cut(s) 2907
HaeIII GGCC 7 cut(s) 84, 701, 2239, 3307, 3684, 4299, 4740
HapII CCGG 8 cut(s) 27, 42, 75, 85, 2106, 2697, 2798, 3831
HgaI GACGC 2 cut(s) 94, 441
HhaI GCGC 5 cut(s) 1265, 1295, 1590, 2906, 4766
Hin1I GRCGYC 1 cut(s) 105
Hin6I GCGC 5 cut(s) 1263, 1293, 1588, 2904, 4764
HinP1I GCGC 5 cut(s) 1263, 1293, 1588, 2904, 4764
HincII GTYRAC 3 cut(s) 367, 475, 2621
HindII GTYRAC 3 cut(s) 367, 475, 2621
HindIII AAGCTT 4 cut(s) 1015, 1760, 2006, 3633
HpaII CCGG 8 cut(s) 27, 42, 75, 85, 2106, 2697, 2798, 3831
Hpy166II GTNNAC 8 cut(s) 367, 475, 754, 2413, 2621, 3119, 3146, 4669
Hpy8I GTNNAC 8 cut(s) 367, 475, 754, 2413, 2621, 3119, 3146, 4669
Hpy99I CGWCG 3 cut(s) 65, 146, 155
HpyCH4IV ACGT 3 cut(s) 1537, 3611, 4129
HpyF3I CTNAG 7 cut(s) 1068, 2016, 2682, 3781, 3892, 4587, 4911
HpySE526I ACGT 3 cut(s) 1537, 3611, 4129
Hsp92I GRCGYC 1 cut(s) 105
HspAI GCGC 5 cut(s) 1263, 1293, 1588, 2904, 4764
KflI GGGWCCC 1 cut(s) 1946
Kpn2I TCCGGA 1 cut(s) 3830
KpnI GGTACC 2 cut(s) 495, 1683
KroI GCCGGC 1 cut(s) 2797
KroNI GCCGGC 1 cut(s) 2799
Ksp22I TGATCA 4 cut(s) 873, 2383, 3232, 4726
KspI CCGCGG 1 cut(s) 103
MaeII ACGT 3 cut(s) 1537, 3611, 4129
MaeIII GTNAC 7 cut(s) 158, 1481, 1880, 2447, 3157, 3560, 3839
MbiI CCGCTC 2 cut(s) 857, 4623
MfeI CAATTG 3 cut(s) 1917, 3940, 4639
MlsI TGGCCA 1 cut(s) 4740
MluNI TGGCCA 1 cut(s) 4740
MlyI GAGTC 4 cut(s) 2111, 2285, 2408, 3182
Mox20I TGGCCA 1 cut(s) 4740
Mph1103I ATGCAT 1 cut(s) 425
MroI TCCGGA 1 cut(s) 3830
MroNI GCCGGC 1 cut(s) 2797
MroXI GAANNNNTTC 3 cut(s) 2808, 4339, 4816
MscI TGGCCA 1 cut(s) 4740
MslI CAYNNNNRTG 1 cut(s) 1746
Msp20I TGGCCA 1 cut(s) 4740
MspA1I CMGCKG 2 cut(s) 102, 2180
MspI CCGG 8 cut(s) 27, 42, 75, 85, 2106, 2697, 2798, 3831
MspR9I CCNGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
MunI CAATTG 3 cut(s) 1917, 3940, 4639
Mva1269I GAATGC 3 cut(s) 442, 2209, 3586
MvaI CCWGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
MvnI CGCG 3 cut(s) 102, 435, 705
NaeI GCCGGC 1 cut(s) 2799
NcoI CCATGG 1 cut(s) 1862
NdeI CATATG 2 cut(s) 1601, 1743
NgoMIV GCCGGC 1 cut(s) 2797
NmuCI GTSAC 3 cut(s) 158, 2447, 3839
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 3 cut(s) 588, 697, 5007
NspV TTCGAA 2 cut(s) 8, 120
PaeI GCATGC 2 cut(s) 588, 5007
PaqCI CACCTGC 1 cut(s) 2825
PcsI WCGNNNNNNNCGW 3 cut(s) 102, 150, 4389
PctI GAATGC 3 cut(s) 442, 2209, 3586
PdiI GCCGGC 1 cut(s) 2799
PdmI GAANNNNTTC 3 cut(s) 2808, 4339, 4816
PflMI CCANNNNNTGG 1 cut(s) 4983
Ple19I CGATCG 1 cut(s) 485
PleI GAGTC 4 cut(s) 2110, 2284, 2408, 3181
PpsI GAGTC 4 cut(s) 2110, 2284, 2408, 3181
PpuMI RGGWCCY 2 cut(s) 1511, 1946
PshAI GACNNNNGTC 1 cut(s) 3172
PshBI ATTAAT 1 cut(s) 576
PsiI TTATAA 1 cut(s) 1767
Psp124BI GAGCTC 2 cut(s) 2075, 4496
Psp5II RGGWCCY 2 cut(s) 1511, 1946
Psp6I CCWGG 6 cut(s) 220, 2357, 2975, 3792, 4788, 5078
PspFI CCCAGC 9 cut(s) 2168, 2397, 2507, 2823, 2886, 3678, 3867, 4629, 4772
PspGI CCWGG 6 cut(s) 220, 2357, 2975, 3792, 4788, 5078
PspOMI GGGCCC 1 cut(s) 699
PspPPI RGGWCCY 2 cut(s) 1511, 1946
PsrI GAACNNNNNNTAC 2 cut(s) 612, 644
PstI CTGCAG 1 cut(s) 4702
PstNI CAGNNNCTG 4 cut(s) 1364, 1394, 2591, 3899
PvuI CGATCG 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 2180
RsaI GTAC 9 cut(s) 59, 493, 838, 999, 1681, 2497, 4246, 4279, 4837
RsaNI GTAC 9 cut(s) 58, 492, 837, 998, 1680, 2496, 4245, 4278, 4836
RseI CAYNNNNRTG 1 cut(s) 1746
SacI GAGCTC 2 cut(s) 2075, 4496
SacII CCGCGG 1 cut(s) 103
SchI GAGTC 4 cut(s) 2111, 2285, 2408, 3182
ScrFI CCNGG 6 cut(s) 222, 2359, 2977, 3794, 4790, 5080
SfcI CTRYAG 5 cut(s) 204, 687, 1672, 4698, 5091
Sfr303I CCGCGG 1 cut(s) 103
SfuI TTCGAA 2 cut(s) 8, 120
SgrBI CCGCGG 1 cut(s) 103
SinI GGWCC 7 cut(s) 1134, 1511, 1634, 1805, 1946, 4669, 4751
SmiMI CAYNNNNRTG 1 cut(s) 1746
SmlI CTYRAG 3 cut(s) 110, 880, 4759
SmoI CTYRAG 3 cut(s) 110, 880, 4759
SphI GCATGC 2 cut(s) 588, 5007
SstI GAGCTC 2 cut(s) 2075, 4496
StyD4I CCNGG 6 cut(s) 220, 2357, 2975, 3792, 4788, 5078
StyI CCWWGG 2 cut(s) 1862, 4432
TaiI ACGT 3 cut(s) 1540, 3614, 4132
TaqI TCGA 8 cut(s) 8, 96, 120, 165, 891, 2264, 4024, 4694
TauI GCSGC 4 cut(s) 48, 105, 150, 3852
TscAI CASTG 8 cut(s) 163, 379, 920, 2005, 2461, 3850, 4566, 4729
TseFI GTSAC 3 cut(s) 158, 2447, 3839
Tsp45I GTSAC 3 cut(s) 158, 2447, 3839
TspGWI ACGGA 6 cut(s) 1147, 1928, 3033, 4242, 4275, 5034
TspRI CASTG 8 cut(s) 163, 379, 920, 2005, 2461, 3850, 4566, 4729
Van91I CCANNNNNTGG 1 cut(s) 4983
VpaK11BI GGWCC 7 cut(s) 1134, 1511, 1634, 1805, 1946, 4669, 4751
VspI ATTAAT 1 cut(s) 576
XceI RCATGY 3 cut(s) 588, 697, 5007
XcmI CCANNNNNNNNNTGG 2 cut(s) 1860, 4980
XmnI GAANNNNTTC 3 cut(s) 2808, 4339, 4816
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.