MD01G1100800.v1.1

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012966
Physical Location & Seq
Reverse (-)
21383190 .. 21390561
7372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1100800.v1.1.491

Sequence Viewer

Length: 4677 bp
ATGGCGTCGAAGGGTAAGGGGATCGCCACCGGAGGTGGATTCTCCGGCGGGAAGCGTAAGCACGATGACAAGTCCGGTGGTGGCCGGAAGAGGGGCAACCCCGGGGTTCTTAGATTCTTCGAAGACGTCGCTGACGAAGCCGACGACGGCGACGAGAGTGACTTCGATGACGATTTTATGGAAGAAGAATTTGAAACAGAACCTGTAGCCCTGAATGACCCAGGGAAAGCACATAACCTTCCATTCGTTCCTAAAGAGGAGGATCTGGATGGTGAAGAATTTGAAAAAATGATGGAAGAACGATACAGGAGTGGCTCGAGCTATGTAACCTATGCTGAAGATAATTATGAGAACAAAAGGTCTGTTGATGGAAATGTTCTGCCTAATGTTAAGGATCCAATCATCTGGAAAGTGAAATGCATGGTTGGCCGCGAGAGGCATTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTCCGATCGTTAGGTACCAAACTACAGATTGTTTCTGCATTTGCTGTTGAGCACATCAAGGGTTTTGTTTTCATCGAAGCTGACAAGCAATCTGATATTAATGAGGCATGTAAGGGAATTTGTAGTATATACCCGTCCCGAGTAATGCCAGTTCCAAATAACGAAGTTTCTCATTTGCTGTCTCCTCGGACTAAATCCAATGGAATTACTGCTGGCATGTGGGCTCGTGTGAAGAATGGGAACTACAAGGGTGATTTGGCACAGGTTGTATTTGTGAACGATTTACGAAAAAGAGCGACCGTAAAGCTGATCCCCAGAATTGATCTTCAAGCAATGGCTGCAAAATTTGGTGGAGGAGGTACTAGAAAGAAAAGGTCTGCTCCTGCCCCAAGATTGATCAGCTCAAACGAACTCGAGGAGTTCCGCCCTCTCATCCAGTACAGGACTGATCGTGAAAGTGGCATGAAGTTTGAATTTCTTGATGGTCTGATGTTCAAGGATGGATATGTATACAAAAAAGTATCAATAGATTCATTAAGCTTCTGGGGTGTTATGCCATCAGATGAAGAGCTACTGAAATTCACTCCTTCTGAGAACACCAAAAATGAGGATGAGGACTGGCTTACAGAGCTTTATGGTACAACAGAAAAGAAAAGGCGGACCATCAAAATTGAAGAAGGCGGTGGTAAAGGAGAGGGTTCATCAGGTTCTATGGGGAAAGGAGAGGGTTCATCAGGGTCTATGGGGAAAGGAGAGGGCACATCAGGGTCTATGGGGAAAGGAGAGGGTTCATCAGGATCTATGGGGAAAGGAGAGGGTTCATCACAGACTATGGGGAAAGGAGAGGGTTCATCTGGGTCTAAGGGGAAAGGAGAGGGTTCATCAGGTTCTATGGGAAAGGGTGAGGACTCATCAGCGTCTATGAGGAAAGGAAAGGGTTCTTCAGTGTCCAGTGGGAATGGCTTTGAACTGTATGATCTTGTTTGTTTTGGCCGGAAAGATTTTGGTCTTGTAATAGGCATGGAGAAAGATGATAGCTACAAGATTCTGAAAGAGGGGGTGGAAGGACCTATTGTGCTGATGGTTCAGAAGCGTGAGTTAAAAAATGTTCTTTCTGATATGAAATTTACGGCATTAGATCAGCACATGAAGGCCATATGCGTTAGTGATACTGTCAAAGTGTTGCAAGGACCTTTGGAGGGTAGGCAGGGTATTGTTAAGCAAATCTATAGAGGCACCATATTCTTGTATGATGAGAACGAGACAGAAAATGGTGGTTATTTTTGCTCAAAATCACAAATGTGTGAGAAAGTTAAGCTTTACATTGATTCCTGCAAAGAAAAGGATGGGGATTCAGGTGCTCTGGGTTTTGGAGATTTCGTGTCGTCTCCTAAATCCCCGCTATCGCCAAAAAAGCCATGGCAAGAAAGGGATAGTAACTTCAGTCAGGGTGATAAAGATGGAATGTTCTCAATTGGTCAAACTGTAAGAATCCGTGTGGGTCCATTGAAGGGATACCTCTGTCGCATCTTGGCTATACGCCGTGCTGATATTACTGTTAAGCTTGATTCTCAGCAAAAGGTTCTTACAGTTACAAAAGAGCATATTTCTGAAGTTCGTGGAAAGAGCTCTAATGTGCTGATTGGTGAGGACCCGGAGTCCAGTTTAAAACCATTTGATTTGCTCGGAAGTGAAGGAGGCTCAAATGACTGGACAAATGGAGCTGGGGCATCTGCTGGGGGTGATGGGTGGAATGCTGGAGGGGCATCAGCCGGGGGTGGTGGGTGGAATGCTGGAGGGGCATCTGGTGAAAGTAATTCTTGGGCTAGTGCAAACCTTGTTAAAAAAGATGATGATACTTCTTGGGGGAGCAAAGCGGCTCCAAGTACAGCTTCATCCTGGGGTGCAGCAGCAGCACCAACAGCAAATAACAATGATCAAGGTGCCGGCTGGGGAAAAAGTGACGCCTGGGGAAAATCAAGTGCTAAAACTGAGGGTGATAGTAGTGCACCAGATAACAGTGATCAAGGTGCTAGCTGGGGAAAAAGCAACACTTGGGGAAAATCAAGTGCTAAAGCTGGAGGTGACAGTAGTGCACCAGATAACAATGATCAAGGTGCTGGCTGGGGAAAAAGCGATGCTTGGGGAAAATCAAGTGCTAAAACTGGGGGTGGCAGCGGTGCACCAGCAGATAACAATGATCAAGGCGCTGGTTGGGGAAAAAGCGACTCTTGGGGGAAATCAAGTGCTAAAACTGGGGGTGACAGCAGTGCCCCAGATAACAATGACCAAGGTGCTGGCTGGGGAAAAAGTGATTCTTGGGGGCAGAAGGCTGAACCTGCTGGGACGGGTCAACTGGATTCATGGGGCAAAGGTAAAAATGTTGTTGATGCGGGAGCATGGGGAAAGAAGTCTGATGCACCATCTGGTGATATTTCAAGCCCTGGGTGGGCTCAACAGAAATCTTGGGACAAAGGAAATGCAGTCGGTAGTGATGGGCCTGCTTGGGGTAAGCCTCAGAATAAAGCCACAGGGAACTGGAGCAGTAAAGATTCACCAACTGTTGGTGATGCAGGGTGGAAAAGCTCAACGCCTGCTGAAAATGTTCAGACTGGAAGCTGGGGTAAGGCAGGTGGCAGTTCAACCGAGAGTAAACAAGATGGCAGTTCTTCTTGGGGTAAGCCAGCTGGAGATTCTTCATGGGGTAAACAAACTGGAGGGTCTTCATGGGGTAAACAAGCTAATGTAACCGCTGCCGGAGTGGGGAACAATGGAGGCAAGTGGGGCAAAGGATCTGCCGCCAATGAACAACAAAGTGGTGGAGGGGGAGATCAATCATCAGGCTGGGGTCAAACTAAAGCTTGGCAAGGTGGGTCAAGTGGTGGAGGGGGAGATCAGGTTTCTAAATGGGGCCAGAAAGGCAATTGGAACTCAGGATCTAGTGAGACTGGTGGCAGTCAAGAGTCTGGTTGGGGTAAGAAAAGCGATGGGAACATCGGATCTGCTTCCTCAGGTGGGAACCAGGATTCTACTTGGGGCAAGATAATCAACTCGAACTCTGGATCTGACGACACTGGTGGAAATCTTGATTCTAGTTGGGGGAAGAAGAGTACTTGGAATTCTCAATCTAGTGAGACTGGTGGCAATCAAGAGTCCAGTTGGGGGAAGAAAAGTGATTGGAAATCAGGATCTGATGACACCGGTGGGAACCAGGATTCAACTTGGGGCAAAAAAAGTAGTTGGAACTCAGGAACTGGTGATGCAGATCAGAAATCTGGTTGGGGCAGTAAAAGCAATTGGAATTCAACAGATGGTGGGAATGGAGACCAGCCTGAGGATTCAAGTGGTGGGAATTGGAGAGGTGGACGTGGTTTTGGAGGTAGAGGCGGTGATAGAGATGGTGACAGAGGGGGTTTTGGAGATGGTGGGCGTAGAGGGGGTTTTGGGGGTAGAGGTGGAGGCGGGAGCAGTGGTGCTTGCTTCAAATGTGGTGAATCTGGGCACATGTCGAGGGAGTGCCCCCAGGGTGGTGGAGGTGGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCTGGGCACATGTCGCGGGAGTGCCCTCAAGGCGGTGGAGGCGGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCTGGACACATGTCGAGGGAGTGCCCACAGGGCGGTGGAGGCGGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCCGGGCACATGTCAAGGGAGTGCCCCAAGGGCGGCGGCGGCAGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCCGGGCACATGTCGAGGGAGTGCCCCCAGGGTGGTGGAGGCGGTGGAGGTGGTGCTTGCTACAAGTGTGGTGAGTCTGGGCACATGGCAAGGGACTGCTCCCAGGGCGGTGGAAGCTATGGTGGCGGCAGCCGTACCGGTGGAGGCGGCGGCGGTGGTGCTTGCTTCAAGTGTGGTGAGTCTGGGCATATGGCGAGGGAGTGCCCCCAGGGCGGTGGAGGGGGTAGCAGTGGTGCTTGCTACAAGTGTGGTGAGTCAGGGCACATGGCGAGGGACTGCTCCCAGGGCGGTGGAGGCTATGGTGGTGGTGGCCGTGGCAGTGGTGGCCGTGGCAGTGGTGGAGGCGGTGGCTGCTACAAGTGTGGACAGTCTGGCCATTTCGCAAGGGAGTGCCCGAACAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1559

Amino Acids

160.26

Weight (kDa)

7.14

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 135 - 218 2.5e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 230 - 352 3e-29 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 476 - 527 5.4e-10 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 536 - 590 6.3e-24 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 650 - 694 1e-07 Transcription elongation factor SPT5, fifth KOW domain
zf-CCHC PF00098 1317 - 1333 8.3e-07 Zinc knuckle
zf-CCHC PF00098 1342 - 1358 8.3e-07 Zinc knuckle
zf-CCHC PF00098 1367 - 1383 8.3e-07 Zinc knuckle
zf-CCHC PF00098 1392 - 1408 9.5e-07 Zinc knuckle
zf-CCHC PF00098 1417 - 1433 8.3e-07 Zinc knuckle
zf-CCHC PF00098 1442 - 1458 5.6e-07 Zinc knuckle
zf-CCHC PF00098 1477 - 1493 4.3e-07 Zinc knuckle
zf-CCHC PF00098 1502 - 1518 5.6e-07 Zinc knuckle
zf-CCHC PF00098 1540 - 1556 8.7e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3095
AatII GACGTC 1 cut(s) 129
Acc36I ACCTGC 2 cut(s) 2821, 3095
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 3 cut(s) 488, 1709, 2417
AccB7I CCANNNNNTGG 1 cut(s) 3038
AccI GTMKAC 1 cut(s) 984
AccII CGCG 2 cut(s) 432, 4060
AcoI YGGCCR 6 cut(s) 82, 427, 1465, 4579, 4594, 4642
AcsI RAATTY 9 cut(s) 188, 278, 591, 818, 947, 1052, 1598, 3592, 3775
AcuI CTGAAG 4 cut(s) 357, 1401, 1900, 2106
AcyI GRCGYC 3 cut(s) 5, 126, 2439
AfaI GTAC 7 cut(s) 490, 835, 914, 1114, 2362, 3586, 4405
AflIII ACRYGT 5 cut(s) 3978, 4053, 4128, 4203, 4278
AgeI ACCGGT 2 cut(s) 3674, 4406
AhdI GACNNNNNGTC 1 cut(s) 2131
AjiI CACGTC 1 cut(s) 3842
AjuI GAANNNNNNNTTGG 2 cut(s) 2350, 2382
AleI CACNNNNGTG 1 cut(s) 1774
Alw21I GWGCWC 6 cut(s) 528, 1837, 2105, 2485, 2572, 2659
Alw26I GTCTC 6 cut(s) 660, 1730, 1866, 3413, 3602, 3792
Alw44I GTGCAC 3 cut(s) 2481, 2568, 2655
AlwNI CAGNNNCTG 3 cut(s) 203, 3665, 3728
Ama87I CYCGRG 4 cut(s) 101, 316, 612, 887
AoxI GGCC 9 cut(s) 82, 427, 1465, 1626, 2972, 3385, 4579, 4594, 4642
ApaLI GTGCAC 3 cut(s) 2481, 2568, 2655
ApeKI GCWGC 8 cut(s) 812, 2381, 2384, 2387, 2649, 3227, 4398, 4620
ApoI RAATTY 9 cut(s) 188, 278, 591, 818, 947, 1052, 1598, 3592, 3775
AseI ATTAAT 1 cut(s) 573
AsiGI ACCGGT 2 cut(s) 3674, 4406
Asp700I GAANNNNTTC 2 cut(s) 1411, 3078
Asp718I GGTACC 1 cut(s) 488
AspLEI GCGC 1 cut(s) 2684
AspS9I GGNCC 7 cut(s) 1134, 1541, 1664, 1976, 2125, 2972, 3385
AsuC2I CCSGG 6 cut(s) 102, 103, 2129, 2248, 4198, 4273
AsuII TTCGAA 1 cut(s) 120
AsuNHI GCTAGC 1 cut(s) 2507
AvaI CYCGRG 4 cut(s) 101, 316, 612, 887
AvaII GGWCC 5 cut(s) 1134, 1541, 1664, 1976, 2125
AxyI CCTNAGG 2 cut(s) 3484, 3807
BaeI ACNNNNGTAYC 4 cut(s) 295, 328, 1981, 2014
BalI TGGCCA 1 cut(s) 4644
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 3 cut(s) 488, 1709, 2417
BanII GRGCYC 3 cut(s) 700, 2105, 2929
BauI CACGAG 1 cut(s) 699
BbsI GAAGAC 2 cut(s) 129, 3189
Bbv12I GWGCWC 6 cut(s) 528, 1837, 2105, 2485, 2572, 2659
BbvI GCAGC 8 cut(s) 799, 2393, 2396, 2399, 2661, 3214, 4410, 4607
BceAI ACGGC 6 cut(s) 163, 1620, 2001, 4386, 4566, 4581
BciVI GTATCC 1 cut(s) 1982
BclI TGATCA 5 cut(s) 870, 2410, 2497, 2584, 2674
BcnI CCSGG 6 cut(s) 102, 103, 2129, 2248, 4198, 4273
BcoDI GTCTC 6 cut(s) 660, 1730, 1866, 3413, 3602, 3792
BfaI CTAG 6 cut(s) 837, 2301, 2508, 3414, 3567, 3603
BfmI CTRYAG 3 cut(s) 204, 497, 1702
BfoI RGCGCY 1 cut(s) 2685
BfuAI ACCTGC 2 cut(s) 2821, 3095
BfuI GTATCC 1 cut(s) 1982
BglI GCCNNNNNGGC 5 cut(s) 3393, 4074, 4149, 4224, 4479
BmcAI AGTACT 1 cut(s) 3586
Bme18I GGWCC 5 cut(s) 1134, 1541, 1664, 1976, 2125
BmeRI GACNNNNNGTC 1 cut(s) 2131
BmeT110I CYCGRG 4 cut(s) 101, 316, 612, 887
BmgBI CACGTC 1 cut(s) 3842
BmgT120I GGNCC 7 cut(s) 1134, 1541, 1664, 1976, 2125, 2972, 3385
BmrI ACTGGG 2 cut(s) 2649, 2739
BmtI GCTAGC 1 cut(s) 2511
BmuI ACTGGG 2 cut(s) 2649, 2739
BoxI GACNNNNGTC 1 cut(s) 4129
BpiI GAAGAC 2 cut(s) 129, 3189
BpmI CTGGAG 6 cut(s) 2253, 2289, 2574, 3034, 3183, 3210
Bpu14I TTCGAA 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 4056
BpuMI CCSGG 6 cut(s) 102, 103, 2129, 2248, 4198, 4273
BsaBI GATNNNNATC 1 cut(s) 3738
BsaHI GRCGYC 3 cut(s) 5, 126, 2439
BsaI GGTCTC 1 cut(s) 3792
BsaWI WCCGGW 4 cut(s) 29, 74, 3674, 4406
BsaXI ACNNNNNCTCC 6 cut(s) 1839, 1869, 3357, 3387, 3931, 3961
Bse118I RCCGGY 3 cut(s) 2420, 3674, 4406
Bse21I CCTNAGG 2 cut(s) 3484, 3807
Bse3DI GCAATG 1 cut(s) 813
Bse8I GATNNNNATC 1 cut(s) 3738
BseGI GGATG 6 cut(s) 274, 906, 979, 1090, 1825, 2369
BseJI GATNNNNATC 1 cut(s) 3738
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 8 cut(s) 1056, 2060, 2457, 3005, 3420, 3498, 3735, 3798
BseRI GAGGAG 4 cut(s) 272, 648, 843, 905
BseXI GCAGC 8 cut(s) 799, 2393, 2396, 2399, 2661, 3214, 4410, 4607
BseYI CCCAGC 9 cut(s) 2198, 2210, 2424, 2511, 2598, 2775, 2816, 3093, 3318
BsgI GTGCAG 1 cut(s) 2400
Bsh1236I CGCG 2 cut(s) 432, 4060
Bsh1285I CGRYCG 2 cut(s) 482, 774
BshFI GGCC 9 cut(s) 84, 429, 1467, 1628, 2974, 3387, 4581, 4596, 4644
BshNI GGYRCC 3 cut(s) 488, 1709, 2417
BshTI ACCGGT 2 cut(s) 3674, 4406
BsiEI CGRYCG 2 cut(s) 482, 774
BsiHKAI GWGCWC 6 cut(s) 528, 1837, 2105, 2485, 2572, 2659
BsiHKCI CYCGRG 4 cut(s) 101, 316, 612, 887
BslFI GGGAC 5 cut(s) 595, 2833, 2957, 4376, 4556
BsmAI GTCTC 6 cut(s) 660, 1730, 1866, 3413, 3602, 3792
BsmBI CGTCTC 1 cut(s) 1866
BsmFI GGGAC 5 cut(s) 595, 2833, 2957, 4376, 4556
BsmI GAATGC 3 cut(s) 439, 2233, 2269
BsnI GGCC 9 cut(s) 84, 429, 1467, 1628, 2974, 3387, 4581, 4596, 4644
Bso31I GGTCTC 1 cut(s) 3792
BsoBI CYCGRG 4 cut(s) 101, 316, 612, 887
Bsp119I TTCGAA 1 cut(s) 120
Bsp19I CCATGG 1 cut(s) 1892
BspANI GGCC 9 cut(s) 84, 429, 1467, 1628, 2974, 3387, 4581, 4596, 4644
BspCNI CTCAG 8 cut(s) 1057, 2059, 2458, 3004, 3419, 3497, 3734, 3799
BspFNI CGCG 2 cut(s) 432, 4060
BspMI ACCTGC 2 cut(s) 2821, 3095
BspOI GCTAGC 1 cut(s) 2511
BspQI GCTCTTC 1 cut(s) 1035
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 3 cut(s) 488, 1709, 2417
BspTNI GGTCTC 1 cut(s) 3792
BsrDI GCAATG 1 cut(s) 813
BsrFI RCCGGY 3 cut(s) 2420, 3674, 4406
BssAI RCCGGY 3 cut(s) 2420, 3674, 4406
BssNAI GTATAC 1 cut(s) 985
BssNI GRCGYC 3 cut(s) 5, 126, 2439
BssSI CACGAG 1 cut(s) 699
BssT1I CCWWGG 3 cut(s) 1892, 2764, 4221
Bst1107I GTATAC 1 cut(s) 985
Bst2BI CACGAG 1 cut(s) 699
Bst6I CTCTTC 3 cut(s) 83, 1035, 3575
BstACI GRCGYC 3 cut(s) 5, 126, 2439
BstAPI GCANNNNNTGC 1 cut(s) 812
BstBI TTCGAA 1 cut(s) 120
BstDSI CCRYGG 3 cut(s) 1892, 4582, 4597
BstF5I GGATG 6 cut(s) 274, 906, 979, 1090, 1825, 2369
BstFNI CGCG 2 cut(s) 432, 4060
BstH2I RGCGCY 1 cut(s) 2685
BstHHI GCGC 1 cut(s) 2684
BstMAI GTCTC 6 cut(s) 660, 1730, 1866, 3413, 3602, 3792
BstMCI CGRYCG 2 cut(s) 482, 774
BstNSI RCATGY 7 cut(s) 585, 694, 3982, 4057, 4132, 4207, 4282
BstPAI GACNNNNGTC 1 cut(s) 4129
BstSFI CTRYAG 3 cut(s) 204, 497, 1702
BstUI CGCG 2 cut(s) 432, 4060
BstV1I GCAGC 8 cut(s) 799, 2393, 2396, 2399, 2661, 3214, 4410, 4607
BstV2I GAAGAC 2 cut(s) 129, 3189
BstX2I RGATCY 8 cut(s) 262, 394, 1271, 3266, 3410, 3473, 3536, 3662
BstXI CCANNNNNNTGG 7 cut(s) 405, 2771, 4004, 4304, 4379, 4484, 4559
BstYI RGATCY 8 cut(s) 262, 394, 1271, 3266, 3410, 3473, 3536, 3662
BstZ17I GTATAC 1 cut(s) 985
Bsu36I CCTNAGG 2 cut(s) 3484, 3807
BsuI GTATCC 1 cut(s) 1982
BsuRI GGCC 9 cut(s) 84, 429, 1467, 1628, 2974, 3387, 4581, 4596, 4644
BtgI CCRYGG 3 cut(s) 1892, 4582, 4597
BtgZI GCGATG 2 cut(s) 2625, 3474
BtrI CACGTC 1 cut(s) 3842
BtsCI GGATG 6 cut(s) 274, 906, 979, 1090, 1825, 2369
BtsI GCAGTG 5 cut(s) 2749, 3949, 4504, 4594, 4609
BveI ACCTGC 2 cut(s) 2821, 3095
CaiI CAGNNNCTG 3 cut(s) 203, 3665, 3728
CfoI GCGC 1 cut(s) 2684
Cfr10I RCCGGY 3 cut(s) 2420, 3674, 4406
Cfr13I GGNCC 7 cut(s) 1134, 1541, 1664, 1976, 2125, 2972, 3385
Cfr9I CCCGGG 1 cut(s) 101
CseI GACGC 2 cut(s) 1380, 2447
Csp6I GTAC 7 cut(s) 489, 834, 913, 1113, 2361, 3585, 4404
CspAI ACCGGT 2 cut(s) 3674, 4406
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviQI GTAC 7 cut(s) 489, 834, 913, 1113, 2361, 3585, 4404
DraI TTTAAA 1 cut(s) 2142
DriI GACNNNNNGTC 1 cut(s) 2131
EaeI YGGCCR 6 cut(s) 82, 427, 1465, 4579, 4594, 4642
Eam1104I CTCTTC 3 cut(s) 83, 1035, 3575
Eam1105I GACNNNNNGTC 1 cut(s) 2131
EarI CTCTTC 3 cut(s) 83, 1035, 3575
EciI GGCGGA 2 cut(s) 887, 1147
Ecl136II GAGCTC 1 cut(s) 2103
Eco130I CCWWGG 3 cut(s) 1892, 2764, 4221
Eco24I GRGCYC 3 cut(s) 700, 2105, 2929
Eco31I GGTCTC 1 cut(s) 3792
Eco47I GGWCC 5 cut(s) 1134, 1541, 1664, 1976, 2125
Eco53kI GAGCTC 1 cut(s) 2103
Eco57I CTGAAG 4 cut(s) 357, 1401, 1900, 2106
Eco81I CCTNAGG 2 cut(s) 3484, 3807
Eco88I CYCGRG 4 cut(s) 101, 316, 612, 887
EcoICRI GAGCTC 1 cut(s) 2103
EcoO109I RGGNCCY 3 cut(s) 1541, 1664, 2125
EcoRI GAATTC 2 cut(s) 3592, 3775
EcoT14I CCWWGG 3 cut(s) 1892, 2764, 4221
EcoT22I ATGCAT 1 cut(s) 422
EcoT38I GRGCYC 3 cut(s) 700, 2105, 2929
ErhI CCWWGG 3 cut(s) 1892, 2764, 4221
Esp3I CGTCTC 1 cut(s) 1866
FaqI GGGAC 5 cut(s) 595, 2833, 2957, 4376, 4556
FauI CCCGC 5 cut(s) 41, 1881, 2860, 3929, 4053
FauNDI CATATG 2 cut(s) 1631, 4458
FbaI TGATCA 5 cut(s) 870, 2410, 2497, 2584, 2674
FblI GTMKAC 1 cut(s) 984
FokI GGATG 6 cut(s) 281, 893, 986, 1097, 1832, 2356
FriOI GRGCYC 3 cut(s) 700, 2105, 2929
FspBI CTAG 6 cut(s) 837, 2301, 2508, 3414, 3567, 3603
GlaI GCGC 1 cut(s) 2683
GsaI CCCAGC 9 cut(s) 2202, 2214, 2428, 2515, 2602, 2779, 2820, 3097, 3322
GsuI CTGGAG 6 cut(s) 2253, 2289, 2574, 3034, 3183, 3210
HaeII RGCGCY 1 cut(s) 2685
HaeIII GGCC 9 cut(s) 84, 429, 1467, 1628, 2974, 3387, 4581, 4596, 4644
HgaI GACGC 2 cut(s) 1380, 2447
HhaI GCGC 1 cut(s) 2684
Hin1I GRCGYC 3 cut(s) 5, 126, 2439
Hin6I GCGC 1 cut(s) 2682
HinP1I GCGC 1 cut(s) 2682
HincII GTYRAC 2 cut(s) 472, 2828
HindII GTYRAC 2 cut(s) 472, 2828
HindIII AAGCTT 4 cut(s) 1012, 1790, 2036, 3333
Hpy99I CGWCG 5 cut(s) 10, 131, 146, 149, 155
HpyAV CCTTC 9 cut(s) 4, 248, 1071, 1145, 1532, 1618, 1978, 2162, 2797
HpyCH4IV ACGT 2 cut(s) 126, 3841
HpySE526I ACGT 2 cut(s) 126, 3841
Hsp92I GRCGYC 3 cut(s) 5, 126, 2439
HspAI GCGC 1 cut(s) 2682
KpnI GGTACC 1 cut(s) 492
KroI GCCGGC 1 cut(s) 2420
KroNI GCCGGC 1 cut(s) 2422
Ksp22I TGATCA 5 cut(s) 870, 2410, 2497, 2584, 2674
LguI GCTCTTC 1 cut(s) 1035
LmnI GCTCC 9 cut(s) 859, 2195, 2343, 2359, 2870, 3015, 3939, 4373, 4553
Lsp1109I GCAGC 8 cut(s) 799, 2393, 2396, 2399, 2661, 3214, 4410, 4607
MaeI CTAG 6 cut(s) 837, 2301, 2508, 3414, 3567, 3603
MaeII ACGT 2 cut(s) 126, 3841
MaeIII GTNAC 9 cut(s) 158, 325, 1910, 2065, 2435, 2558, 2735, 3220, 3875
MfeI CAATTG 3 cut(s) 1947, 3397, 3769
MflI RGATCY 8 cut(s) 262, 394, 1271, 3266, 3410, 3473, 3536, 3662
MlsI TGGCCA 1 cut(s) 4644
MluNI TGGCCA 1 cut(s) 4644
MmeI TCCRAC 1 cut(s) 3695
Mox20I TGGCCA 1 cut(s) 4644
Mph1103I ATGCAT 1 cut(s) 422
MroNI GCCGGC 1 cut(s) 2420
MroXI GAANNNNTTC 2 cut(s) 1411, 3078
MscI TGGCCA 1 cut(s) 4644
MslI CAYNNNNRTG 1 cut(s) 1774
Msp20I TGGCCA 1 cut(s) 4644
MspA1I CMGCKG 3 cut(s) 2652, 3161, 3227
MunI CAATTG 3 cut(s) 1947, 3397, 3769
Mva1269I GAATGC 3 cut(s) 439, 2233, 2269
MvnI CGCG 2 cut(s) 432, 4060
NaeI GCCGGC 1 cut(s) 2422
NciI CCSGG 6 cut(s) 102, 103, 2129, 2248, 4198, 4273
NcoI CCATGG 1 cut(s) 1892
NdeI CATATG 2 cut(s) 1631, 4458
NgoMIV GCCGGC 1 cut(s) 2420
NheI GCTAGC 1 cut(s) 2507
NmuCI GTSAC 5 cut(s) 158, 2435, 2558, 2735, 3875
NsiI ATGCAT 1 cut(s) 422
NspI RCATGY 7 cut(s) 585, 694, 3982, 4057, 4132, 4207, 4282
NspV TTCGAA 1 cut(s) 120
OliI CACNNNNGTG 1 cut(s) 1774
PaeR7I CTCGAG 2 cut(s) 316, 887
PaqCI CACCTGC 1 cut(s) 3095
PasI CCCWGGG 7 cut(s) 221, 2918, 3997, 4297, 4372, 4477, 4552
PciI ACATGT 5 cut(s) 3978, 4053, 4128, 4203, 4278
PciSI GCTCTTC 1 cut(s) 1035
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 141, 150
PctI GAATGC 3 cut(s) 439, 2233, 2269
PdiI GCCGGC 1 cut(s) 2422
PdmI GAANNNNTTC 2 cut(s) 1411, 3078
PflMI CCANNNNNTGG 1 cut(s) 3038
PinAI ACCGGT 2 cut(s) 3674, 4406
Ple19I CGATCG 1 cut(s) 482
PpuMI RGGWCCY 3 cut(s) 1541, 1664, 2125
PscI ACATGT 5 cut(s) 3978, 4053, 4128, 4203, 4278
PshAI GACNNNNGTC 1 cut(s) 4129
PshBI ATTAAT 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 2105
Psp5II RGGWCCY 3 cut(s) 1541, 1664, 2125
PspFI CCCAGC 9 cut(s) 2198, 2210, 2424, 2511, 2598, 2775, 2816, 3093, 3318
PspPI GGNCC 7 cut(s) 1134, 1541, 1664, 1976, 2125, 2972, 3385
PspPPI RGGWCCY 3 cut(s) 1541, 1664, 2125
PspXI VCTCGAGB 2 cut(s) 316, 887
PsrI GAACNNNNNNTAC 2 cut(s) 609, 641
PstNI CAGNNNCTG 3 cut(s) 203, 3665, 3728
PsuI RGATCY 8 cut(s) 262, 394, 1271, 3266, 3410, 3473, 3536, 3662
PvuI CGATCG 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 3161
RsaI GTAC 7 cut(s) 490, 835, 914, 1114, 2362, 3586, 4405
RsaNI GTAC 7 cut(s) 489, 834, 913, 1113, 2361, 3585, 4404
RseI CAYNNNNRTG 1 cut(s) 1774
SacI GAGCTC 1 cut(s) 2105
SapI GCTCTTC 1 cut(s) 1035
Sau96I GGNCC 7 cut(s) 1134, 1541, 1664, 1976, 2125, 2972, 3385
ScaI AGTACT 1 cut(s) 3586
SfcI CTRYAG 3 cut(s) 204, 497, 1702
Sfr274I CTCGAG 2 cut(s) 316, 887
SfuI TTCGAA 1 cut(s) 120
SgrAI CRCCGGYG 1 cut(s) 3674
SinI GGWCC 5 cut(s) 1134, 1541, 1664, 1976, 2125
SlaI CTCGAG 2 cut(s) 316, 887
SmaI CCCGGG 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 1774
SmlI CTYRAG 3 cut(s) 316, 887, 4071
SmoI CTYRAG 3 cut(s) 316, 887, 4071
SspMI CTAG 6 cut(s) 837, 2301, 2508, 3414, 3567, 3603
SstI GAGCTC 1 cut(s) 2105
StyI CCWWGG 3 cut(s) 1892, 2764, 4221
TaiI ACGT 2 cut(s) 129, 3844
TatI WGTACW 3 cut(s) 912, 2360, 3584
TauI GCSGC 9 cut(s) 432, 2354, 3275, 4230, 4233, 4236, 4398, 4419, 4422
TseFI GTSAC 5 cut(s) 158, 2435, 2558, 2735, 3875
TseI GCWGC 8 cut(s) 812, 2381, 2384, 2387, 2649, 3227, 4398, 4620
Tsp45I GTSAC 5 cut(s) 158, 2435, 2558, 2735, 3875
TspGWI ACGGA 1 cut(s) 1958
TspMI CCCGGG 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 3038
VneI GTGCAC 3 cut(s) 2481, 2568, 2655
VpaK11BI GGWCC 5 cut(s) 1134, 1541, 1664, 1976, 2125
VspI ATTAAT 1 cut(s) 573
XapI RAATTY 9 cut(s) 188, 278, 591, 818, 947, 1052, 1598, 3592, 3775
XceI RCATGY 7 cut(s) 585, 694, 3982, 4057, 4132, 4207, 4282
XcmI CCANNNNNNNNNTGG 1 cut(s) 1890
XhoI CTCGAG 2 cut(s) 316, 887
XmaI CCCGGG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 984
XmnI GAANNNNTTC 2 cut(s) 1411, 3078
XspI CTAG 6 cut(s) 837, 2301, 2508, 3414, 3567, 3603
ZraI GACGTC 1 cut(s) 127
ZrmI AGTACT 1 cut(s) 3586
Zsp2I ATGCAT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.