Rh1AG305300

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0012966
Physical Location & Seq
Reverse (-)
53772775 .. 53782865
10091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG305300.1

Sequence Viewer

Length: 4749 bp
ATGGCAAAGCCATTCCAAAGCACAACGAAGGGCAGAGTCGTAATTTCACTCACAGAGCTCACTGGACCAACATACAATTTAGCCTGCGACCCCGTCACTATTCACGGTACGAGAGAGAGAGAGGAGCTCAGGGAAATTCGAAACGTTTCGCTTTCTCTGTCTACATGCAATTTGAGCTCTGAAACCCTCGCATTGTTGATGGCCTCGAAGGAAAAAGGAAAGGCGATCGCCACAAGCGGATCGTCCGCCGCCGGCAAGCGGAAGCCCGGCGAGGATGACAAGTCCGGCGGCGGCAGTCGGAAGCGGAGAAACCCCGGCGTTCTCCAGTTCTTCGAGCACTCCGCCGCCGAAGCTGACGGTTCGGACGACGACAGCGACTTGGACGATTTTATGGAAGAAGAACTTGAAACAGAACCTATAGTCAAGAGTGAACCGGGGAAAGCCCATAACCTTCCATTTGTTCCCAAAGAGGAGGATATCGATGGGGACGAATTTGAAAGAATGATGGAAGAACGATACAGGACTGGTTCTACATATGTTACCTATGCTGAAGATAATTATGAAAACAAAAGATCGGTTGATGGAATTGTGCTTGAGCCTTCTGCTAAAGATCCTGTTATCTGGAAAGTAAAGTGTGCGGTTGGACGCGAGAGACACTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTACGATCAATGGGTACCAAGCTGCAGATAATATCTGCATTTGCTGTTGATCACATCAAGGGTTTTGTTTTCATTGAAGCTGATAAGCTATGTGACGTTCAGGAGGCATGTAAAGGACTTTGTAATATATTTTTATCCCGAGTAACACCAGTTCCCAAAAATGAAGCTCCTCATTTACTGGCTCCTCGGACTAGAAACAGTGAAATTGCTGTGGGTACATGGGCCCGTGTGAAGAGTGGGAACTACAAGGGTGACTTGGCACAGGTTGTGGCTGTGAACGAAAAGAAAAAAGCAACAGTAAAGCTGATACCAAGAATTGATCTACAAGCAATGGCTGCAAAATTTGGTGGGGGAGTTACTAGAAAGAAACTGCCTACTCCAGCACCGAGATTGATCAGCACAAGCGAACTTGAGGAGTTCCGCCCTCTCATTCAGGACAGGAAGGATAAGGATACCGGATTGCGTTTTCTCTGTCTTGATGGTTTGCTCCTTAAGGATGGATATTTATACAAAAAAATGCCTTTAGATTCATTAAATTGCTGGGGTGTCATGCCATCAGAAGAGGAGATACTGAAATTCAAGCCTTCCGAGAACAATGAATCTGCTGATCTGGAGTGGCTTTCACAGCTTTATGGTGACAATAAGAAAAGGAAGAGCATTGGCAGTGATAAAGGGGATGGGAAAGGAGAAGGTTCAGCAGGGTGTGGGGGGAAAGGAGAAGGTTCATCAAAGGGTAACGGGAAAGGAGAAGGTCCTTCAGGTGGGAAAGAAGGTTCTTCAGGGTTTACTGGGAAAGGAGAAGGTTCTTCAGGGTGTGGTGGGAATCTCTATGAGCTATATGATCTTGTTTGTTTCGGCAAGAAAGATTTTGGTCTTGTGCTAGGCATTGAGAAAGATAATACTTACAAGATTCTGAAAGAGGGTGTGGAAGGATCTACTGTTGTGACAGTTCAGCAGAATGAGATAAAGAATGTACTTTCTGATGTAAAATTTACAGCTTTAGATCAGCACCTGAAGCCCATTTGCGTCAATGATACAGTTAAAGTTTTGGAAGGACCATTGAAGGATAGGCAAGGAATAGTGAAGCTAGTCTACAGAGGCACAATATTCATGTTTGATGAGAATGAGACAGAAAATGGTGGTTATTTCTGCTCAAAATCTCAAATGTGTGAGAAAATCAAGCTTTCCAAGCTTTCCATTGATGTTATCCCAGAAAAGGATGGGGATTCGGGTGCTTTCGGTTTTGAAGATTTCACATCATCACCTAAATCCCCTCTATCGCCAAAAAAGCCTTGGCTAGGAAGGGAGAATAACTTCAATCAGGGCGATACTGACGGAATGTTCTCAATCGGTCAAACTTTAAGAATTCGTGTTGGTCCATTAAAGGGATACCTCTGTCGTGTCTTGGCCATACGCCGTTCTGATATCACAGTTAAGCTTGATTCTCAGCAAAGGGTTCTTACAGTTAAAGCTGAGCATCTTACAGAGGTTCGTGTAAAGAGCGCTGCCATGCTTAGTGAGGATCCGGAGTCCAGCTCTCTTAAACCATTTGATTTACTTGGAACTGAAGGAGGCTCTAAAGATTGGACAGATGGAGCTGGGACATCAGCCGGGGGTGATGGGTGGAATGCTGGAGGGTCATCTGGTGAAAGGAATGCTTGGCCTAGTTTCTCTGCCTCGGGCAATTCGCTTCAGCCTGAGTCCAGCTCTGCGAATCCTTTTGAATCTGATGGAAATGGTGCTAAAAAAGAGGATAGTTCTTGGGAGAGGAACGTGGCTTCAAATAACAACTCATCCTGGGGTGTTGCAGCAGCAGAAAACAAGGATCAAGTTGCTGGCCCGGGAAAAAGCAACTCTTGGGGCGCTAAAGTTGGAGATGACAGCACTTCGTCGGATACTTGGCAAAAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGCAGCAGCTGTAAATAACAAGGATCAAGGTACTGGCTGGGGAAAAAGCGATTCTTGGGGCGCTAAAGTTGGAGGTGACAGCAGTCTGTCAGATACTTTGCAAAAAGCATCAGAACCTTCCAGTTCATCCTGGGGTGTTGCAGCAGCAGTAGATAACAAGGATCAAGGTACTGGCTGGGGAAAAAGTGACTCTTGGGGCGCTAAAGTTGGAGGTGACAGCACTTCGTCGGATACTTGGAAAAAAGCATCAGCGCCTGCTAGTTCATCCTGGGGTGTTGCGGCAGCAATAGATAAGAAGGATCAAGGTACTGGCTGGGGTGGTAAGACTGGATGTGACGGCACTGCATCAGATAGTTGGAAAAAAGCATCAGAACCTGCTAGTAATTCGTCCTGGGGTGTTGCAGCAGCAGCAGATAACAAGGATCAAGGTTCCGTCTGGGGCGGTAGTGGTAGTCAAAAACCGAGTGTTGCAGATGATAGTGGTGACAAAGGTTCTGCTTGGAATAAGCCAGCTGAAGGTTCTTGGAGTAAACAAACTGGAGGATCCTCATGGGGTAAGCAAGCCGATTGGAGCACTAAAGAGGGATCAAGTACTGGTGATGCAGGGTGGAAAAGTTCAACGCCTTCAGTAGAGAATCAGACTGGGAGCTGGGGTAATGCAGGTGGCTGGGCAAAAGCTCAAGGCATATCTGGTAATGAGAATCATAAAGACAGTTGGAAGAAACCAAGTGTTATAGATGACAATAAAAATGCTTCGTGGATCAAGGAAGATGGAGGTTCTACCTGGAATAAACAAGATGGAGGTTCTACTTGGAATAAGCCTGCTGGAGGTTCTTGGAGTAAAGGTGGGGATCAAGGGTCTGGTGGTGGCTATGGTGGCGATGGTGGTGGTGGTAGAGGGGGTGGAGGTGGTAACTGTTTCAAGTGCGGTGAGTCTGGACATATGTCAAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGGCAGGAGGTGGTAACTGTTTCAAGTGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCAGTGGTGGTAGAGGGGCTGGAGGTGGGAACTGTTACAAGTGTGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCGGCGGTGGTGGTAGAGGGAGTGGGGGTGGGAACTGTTTCAAATGTGGTGAGTCTGGACACATGTCTAGGGAGTGTCCTCAGGGCGGCGGTGGTAGAGGGGCTGGAAGTGGGAACTGTTTCAAGTGTGGTGAGTCTGGACACATGGCTAGGGACTGCTCTCAGGGAGGTGGTAGCTATGGCAACTTCGGTGGTGGAGCAAAGTCCACTGATTCATGGAGCAAGCGTTCTTGGGGTACTGGAGATGGTGGAAGTGGCGGTGATGGAGCAAAACCAGCAGCAAGCTCTTGGGGTACCACAGATGGTGGAAGTGGCTTTGGTGGTGGAGCAAAGCCCACTGATTCATGGAGCAAGCGTGCTTGGGGTACTGGAGATGGTGGAAGTGGTAGCGGTGGACCAAAACCAGCAGCAAGCTCTTGGGGTACAAATGGTGGAAGTGGCGGAGCTGGTGGAAGTGGCAGCGGTGGACCAAAACCAGCAGCAAGCTCTTGGGGTACAAATGGTGGAAGTGGCGGAGCTGATGGTGAAGCAAAACCAGCAGCAAGCTTTTGGGGTAAGGGGGATGGTGGAAGTGGTGGACATAGTGATGAAGCAAAACCAGCAGCAAGCACTTGGGGTACAGGAAGCTCTTGGGGTAAGGCAGATGGTGGAAGTGGCAGAGCTAGTGGTGAAGCAAAACCAGCAGCAAGCACTTGGGGTACAGGAAGCTCCTGGGGTAAGGGAGATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAACCAGCAGCAAGCACTTGGGGTAAGGGAGATGGTGGAAGTGGCGGAGATGGTGGTGAAGCAAAGCCTGCTGATCCATGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAGGGAGATGGCGGAAGTGGCGGAGCTAGTGGTGAAGCAAAGCCTGCTGATCCTTGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAGAAAGATGGTGGAAGTGGCAGCGGAGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1582

Amino Acids

162.69

Weight (kDa)

6.26

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 207 - 290 1.9e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 300 - 423 1e-25 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 504 - 554 1.5e-09 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 563 - 617 3.7e-21 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 680 - 724 2e-06 Transcription elongation factor SPT5, fifth KOW domain
zf-CCHC PF00098 1182 - 1198 2e-06 Zinc knuckle
zf-CCHC PF00098 1210 - 1226 2e-06 Zinc knuckle
zf-CCHC PF00098 1238 - 1254 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1267 - 1283 2e-06 Zinc knuckle
zf-CCHC PF00098 1294 - 1310 2.7e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3281
Acc36I ACCTGC 3 cut(s) 2617, 3013, 3281
Acc65I GGTACC 2 cut(s) 704, 4058
AccB1I GGYRCC 2 cut(s) 704, 4058
AccI GTMKAC 2 cut(s) 161, 1782
AccII CGCG 1 cut(s) 648
AccIII TCCGGA 1 cut(s) 2215
AclI AACGTT 1 cut(s) 144
AcoI YGGCCR 1 cut(s) 2097
AcsI RAATTY 6 cut(s) 135, 491, 1031, 1265, 1679, 2055
AcuI CTGAAG 9 cut(s) 570, 1431, 1452, 1482, 1724, 2277, 2366, 3165, 3240
AfeI AGCGCT 1 cut(s) 2194
AflII CTTAAG 1 cut(s) 1181
AflIII ACRYGT 3 cut(s) 3657, 3741, 3828
AjnI CCWGG 7 cut(s) 2486, 2621, 2759, 2897, 3020, 3413, 4475
AloI GAACNNNNNNTCC 4 cut(s) 1447, 1479, 3976, 4008
Alw21I GWGCWC 5 cut(s) 60, 129, 179, 339, 3206
Alw26I GTCTC 2 cut(s) 646, 1811
AlwNI CAGNNNCTG 5 cut(s) 1702, 2609, 2639, 2885, 3005
Ama87I CYCGRG 3 cut(s) 828, 2368, 2530
Aor13HI TCCGGA 1 cut(s) 2215
Aor51HI AGCGCT 1 cut(s) 2194
AoxI GGCC 5 cut(s) 201, 912, 2097, 2351, 2527
ApaI GGGCCC 1 cut(s) 916
ApoI RAATTY 6 cut(s) 135, 491, 1031, 1265, 1679, 2055
AsiSI GCGATCGC 1 cut(s) 228
Asp700I GAANNNNTTC 4 cut(s) 145, 2003, 3803, 3884
Asp718I GGTACC 2 cut(s) 704, 4058
AspLEI GCGC 5 cut(s) 2195, 2555, 2693, 2831, 2884
AspS9I GGNCC 9 cut(s) 65, 912, 913, 1442, 1745, 2066, 2528, 4160, 4232
AsuC2I CCSGG 6 cut(s) 267, 315, 435, 2302, 2531, 2532
AsuII TTCGAA 1 cut(s) 139
AvaI CYCGRG 3 cut(s) 828, 2368, 2530
AvaII GGWCC 6 cut(s) 65, 1442, 1745, 2066, 4160, 4232
AxyI CCTNAGG 4 cut(s) 3591, 3675, 3759, 3846
BaeGI GKGCMC 4 cut(s) 916, 3590, 3674, 3758
BaeI ACNNNNGTAYC 2 cut(s) 2071, 2104
BalI TGGCCA 1 cut(s) 2099
BamHI GGATCC 2 cut(s) 2212, 3173
BanI GGYRCC 2 cut(s) 704, 4058
BanII GRGCYC 4 cut(s) 60, 129, 179, 916
BarI GAAGNNNNNNTAC 4 cut(s) 1242, 1274, 1766, 1798
Bbv12I GWGCWC 5 cut(s) 60, 129, 179, 339, 3206
BceAI ACGGC 2 cut(s) 2091, 2983
BciT130I CCWGG 7 cut(s) 2488, 2623, 2761, 2899, 3022, 3415, 4477
BciVI GTATCC 4 cut(s) 1135, 2072, 2578, 2854
BclI TGATCA 2 cut(s) 739, 1083
BcnI CCSGG 6 cut(s) 267, 315, 435, 2302, 2531, 2532
BcoDI GTCTC 2 cut(s) 646, 1811
BfmI CTRYAG 3 cut(s) 417, 713, 1783
BfoI RGCGCY 5 cut(s) 2196, 2556, 2694, 2832, 2885
BfrI CTTAAG 1 cut(s) 1181
BfuAI ACCTGC 3 cut(s) 2617, 3013, 3281
BfuI GTATCC 4 cut(s) 1135, 2072, 2578, 2854
BglI GCCNNNNNGGC 3 cut(s) 3594, 3678, 3762
BlpI GCTNAGC 1 cut(s) 2163
BmcAI AGTACT 1 cut(s) 3223
Bme18I GGWCC 6 cut(s) 65, 1442, 1745, 2066, 4160, 4232
BmeT110I CYCGRG 3 cut(s) 828, 2368, 2530
BmgT120I GGNCC 9 cut(s) 65, 912, 913, 1442, 1745, 2066, 2528, 4160, 4232
BmiI GGNNCC 7 cut(s) 706, 873, 914, 2214, 3061, 3175, 4060
BmrI ACTGGG 2 cut(s) 1488, 3282
BmsI GCATC 8 cut(s) 663, 2176, 2609, 2747, 2885, 2984, 3005, 3220
BmuI ACTGGG 2 cut(s) 1488, 3282
BoxI GACNNNNGTC 5 cut(s) 2712, 3574, 3658, 3742, 3829
BplI GAGNNNNNCTC 8 cut(s) 111, 143, 641, 673, 2210, 2242, 2381, 2413
BpmI CTGGAG 9 cut(s) 308, 1053, 1322, 2343, 3189, 3477, 3723, 4026, 4155
Bpu10I CCTNAGC 1 cut(s) 128
Bpu1102I GCTNAGC 1 cut(s) 2163
Bpu14I TTCGAA 1 cut(s) 139
BpuEI CTTGAG 3 cut(s) 614, 1121, 3294
BpuMI CCSGG 6 cut(s) 267, 315, 435, 2302, 2531, 2532
Bsa29I ATCGAT 1 cut(s) 480
BsaWI WCCGGW 2 cut(s) 1145, 2215
Bse118I RCCGGY 1 cut(s) 251
Bse21I CCTNAGG 4 cut(s) 3591, 3675, 3759, 3846
Bse3DI GCAATG 1 cut(s) 1026
BseAI TCCGGA 1 cut(s) 2215
BseBI CCWGG 7 cut(s) 2488, 2623, 2761, 2899, 3022, 3415, 4477
BseCI ATCGAT 1 cut(s) 480
BseMI GCAATG 1 cut(s) 1026
BseRI GAGGAG 6 cut(s) 137, 485, 849, 864, 1118, 1268
BseSI GKGCMC 4 cut(s) 916, 3590, 3674, 3758
BseYI CCCAGC 7 cut(s) 1230, 2288, 2667, 2805, 2943, 3279, 3297
Bsh1236I CGCG 1 cut(s) 648
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 5 cut(s) 203, 914, 2099, 2353, 2529
BshNI GGYRCC 2 cut(s) 704, 4058
BshVI ATCGAT 1 cut(s) 480
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 5 cut(s) 60, 129, 179, 339, 3206
BsiHKCI CYCGRG 3 cut(s) 828, 2368, 2530
BsiSI CCGG 9 cut(s) 252, 267, 285, 315, 434, 1146, 2216, 2301, 2531
BslFI GGGAC 3 cut(s) 500, 2305, 3932
BsmAI GTCTC 2 cut(s) 646, 1811
BsmFI GGGAC 3 cut(s) 500, 2305, 3932
BsmI GAATGC 2 cut(s) 2323, 2350
BsnI GGCC 5 cut(s) 203, 914, 2099, 2353, 2529
BsoBI CYCGRG 3 cut(s) 828, 2368, 2530
Bsp119I TTCGAA 1 cut(s) 139
Bsp120I GGGCCC 1 cut(s) 912
Bsp1286I GDGCHC 9 cut(s) 60, 129, 179, 339, 916, 3206, 3590, 3674, 3758
Bsp13I TCCGGA 1 cut(s) 2215
Bsp1720I GCTNAGC 1 cut(s) 2163
Bsp19I CCATGG 1 cut(s) 4598
BspANI GGCC 5 cut(s) 203, 914, 2099, 2353, 2529
BspDI ATCGAT 1 cut(s) 480
BspEI TCCGGA 1 cut(s) 2215
BspFNI CGCG 1 cut(s) 648
BspLI GGNNCC 7 cut(s) 706, 873, 914, 2214, 3061, 3175, 4060
BspMAI CTGCAG 1 cut(s) 717
BspMI ACCTGC 3 cut(s) 2617, 3013, 3281
BspQI GCTCTTC 1 cut(s) 1337
BspT104I TTCGAA 1 cut(s) 139
BspT107I GGYRCC 2 cut(s) 704, 4058
BspTI CTTAAG 1 cut(s) 1181
BsrDI GCAATG 1 cut(s) 1026
BsrFI RCCGGY 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 251
BssT1I CCWWGG 3 cut(s) 1982, 4598, 4682
Bst2UI CCWGG 7 cut(s) 2488, 2623, 2761, 2899, 3022, 3415, 4477
Bst6I CTCTTC 3 cut(s) 917, 1245, 1337
BstAFI CTTAAG 1 cut(s) 1181
BstAPI GCANNNNNTGC 3 cut(s) 1025, 4589, 4673
BstBI TTCGAA 1 cut(s) 139
BstDSI CCRYGG 1 cut(s) 4598
BstENI CCTNNNNNAGG 2 cut(s) 1986, 3456
BstFNI CGCG 1 cut(s) 648
BstH2I RGCGCY 5 cut(s) 2196, 2556, 2694, 2832, 2885
BstHHI GCGC 5 cut(s) 2195, 2555, 2693, 2831, 2884
BstMAI GTCTC 2 cut(s) 646, 1811
BstMCI CGRYCG 1 cut(s) 228
BstNI CCWGG 7 cut(s) 2488, 2623, 2761, 2899, 3022, 3415, 4477
BstNSI RCATGY 5 cut(s) 168, 801, 3661, 3745, 3832
BstPAI GACNNNNGTC 5 cut(s) 2712, 3574, 3658, 3742, 3829
BstSFI CTRYAG 3 cut(s) 417, 713, 1783
BstSLI GKGCMC 4 cut(s) 916, 3590, 3674, 3758
BstUI CGCG 1 cut(s) 648
BstX2I RGATCY 4 cut(s) 610, 1622, 2212, 3173
BstYI RGATCY 4 cut(s) 610, 1622, 2212, 3173
Bsu15I ATCGAT 1 cut(s) 480
Bsu36I CCTNAGG 4 cut(s) 3591, 3675, 3759, 3846
BsuI GTATCC 4 cut(s) 1135, 2072, 2578, 2854
BsuRI GGCC 5 cut(s) 203, 914, 2099, 2353, 2529
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 4598
BtgZI GCGATG 1 cut(s) 3525
BtsI GCAGTG 3 cut(s) 1360, 2970, 3691
BtsIMutI CAGTG 7 cut(s) 60, 895, 1360, 2970, 3691, 3972, 4101
BveI ACCTGC 3 cut(s) 2617, 3013, 3281
CaiI CAGNNNCTG 5 cut(s) 1702, 2609, 2639, 2885, 3005
CfoI GCGC 5 cut(s) 2195, 2555, 2693, 2831, 2884
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 9 cut(s) 65, 912, 913, 1442, 1745, 2066, 2528, 4160, 4232
Cfr9I CCCGGG 1 cut(s) 2530
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 2 cut(s) 654, 1705
CspCI CAANNNNNGTGG 2 cut(s) 3937, 3972
EaeI YGGCCR 1 cut(s) 2097
Eam1104I CTCTTC 3 cut(s) 917, 1245, 1337
EarI CTCTTC 3 cut(s) 917, 1245, 1337
EciI GGCGGA 9 cut(s) 235, 331, 1100, 4221, 4293, 4518, 4581, 4656, 4665
Ecl136II GAGCTC 3 cut(s) 58, 127, 177
Eco130I CCWWGG 3 cut(s) 1982, 4598, 4682
Eco24I GRGCYC 4 cut(s) 60, 129, 179, 916
Eco32I GATATC 2 cut(s) 478, 2116
Eco47I GGWCC 6 cut(s) 65, 1442, 1745, 2066, 4160, 4232
Eco47III AGCGCT 1 cut(s) 2194
Eco53kI GAGCTC 3 cut(s) 58, 127, 177
Eco57I CTGAAG 9 cut(s) 570, 1431, 1452, 1482, 1724, 2277, 2366, 3165, 3240
Eco81I CCTNAGG 4 cut(s) 3591, 3675, 3759, 3846
Eco88I CYCGRG 3 cut(s) 828, 2368, 2530
EcoICRI GAGCTC 3 cut(s) 58, 127, 177
EcoNI CCTNNNNNAGG 2 cut(s) 1986, 3456
EcoO109I RGGNCCY 1 cut(s) 1442
EcoRI GAATTC 1 cut(s) 2055
EcoRII CCWGG 7 cut(s) 2486, 2621, 2759, 2897, 3020, 3413, 4475
EcoRV GATATC 2 cut(s) 478, 2116
EcoT14I CCWWGG 3 cut(s) 1982, 4598, 4682
EcoT38I GRGCYC 4 cut(s) 60, 129, 179, 916
ErhI CCWWGG 3 cut(s) 1982, 4598, 4682
FaqI GGGAC 3 cut(s) 500, 2305, 3932
FauNDI CATATG 2 cut(s) 535, 3573
FbaI TGATCA 2 cut(s) 739, 1083
FblI GTMKAC 2 cut(s) 161, 1782
FriOI GRGCYC 4 cut(s) 60, 129, 179, 916
GlaI GCGC 5 cut(s) 2194, 2554, 2692, 2830, 2883
GsaI CCCAGC 7 cut(s) 1234, 2292, 2671, 2809, 2947, 3283, 3301
GsuI CTGGAG 9 cut(s) 308, 1053, 1322, 2343, 3189, 3477, 3723, 4026, 4155
HaeII RGCGCY 5 cut(s) 2196, 2556, 2694, 2832, 2885
HaeIII GGCC 5 cut(s) 203, 914, 2099, 2353, 2529
HapII CCGG 9 cut(s) 252, 267, 285, 315, 434, 1146, 2216, 2301, 2531
HgaI GACGC 2 cut(s) 654, 1705
HhaI GCGC 5 cut(s) 2195, 2555, 2693, 2831, 2884
Hin6I GCGC 5 cut(s) 2193, 2553, 2691, 2829, 2882
HinP1I GCGC 5 cut(s) 2193, 2553, 2691, 2829, 2882
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 4 cut(s) 1871, 1880, 2126, 4309
HpaII CCGG 9 cut(s) 252, 267, 285, 315, 434, 1146, 2216, 2301, 2531
Hpy99I CGWCG 3 cut(s) 371, 2584, 2860
HpyCH4IV ACGT 3 cut(s) 144, 786, 2463
HpySE526I ACGT 3 cut(s) 144, 786, 2463
HspAI GCGC 5 cut(s) 2193, 2553, 2691, 2829, 2882
Kpn2I TCCGGA 1 cut(s) 2215
KpnI GGTACC 2 cut(s) 708, 4062
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Ksp22I TGATCA 2 cut(s) 739, 1083
LguI GCTCTTC 1 cut(s) 1337
LweI GCATC 8 cut(s) 663, 2176, 2609, 2747, 2885, 2984, 3005, 3220
MaeII ACGT 3 cut(s) 144, 786, 2463
MflI RGATCY 4 cut(s) 610, 1622, 2212, 3173
MhlI GDGCHC 9 cut(s) 60, 129, 179, 339, 916, 3206, 3590, 3674, 3758
MlsI TGGCCA 1 cut(s) 2099
MluNI TGGCCA 1 cut(s) 2099
MlyI GAGTC 9 cut(s) 45, 2228, 2399, 2813, 3572, 3656, 3740, 3827, 3908
MmeI TCCRAC 9 cut(s) 278, 622, 2542, 2562, 2680, 2818, 2838, 2966, 3326
Mox20I TGGCCA 1 cut(s) 2099
MroI TCCGGA 1 cut(s) 2215
MroNI GCCGGC 1 cut(s) 251
MroXI GAANNNNTTC 4 cut(s) 145, 2003, 3803, 3884
MscI TGGCCA 1 cut(s) 2099
MseI TTAA 8 cut(s) 1182, 1223, 1731, 2051, 2072, 2124, 2157, 2232
MslI CAYNNNNRTG 1 cut(s) 4350
Msp20I TGGCCA 1 cut(s) 2099
MspA1I CMGCKG 4 cut(s) 2639, 3143, 4227, 4737
MspCI CTTAAG 1 cut(s) 1181
MspI CCGG 9 cut(s) 252, 267, 285, 315, 434, 1146, 2216, 2301, 2531
Mva1269I GAATGC 2 cut(s) 2323, 2350
MvaI CCWGG 7 cut(s) 2488, 2623, 2761, 2899, 3022, 3415, 4477
MvnI CGCG 1 cut(s) 648
NaeI GCCGGC 1 cut(s) 253
NciI CCSGG 6 cut(s) 267, 315, 435, 2302, 2531, 2532
NcoI CCATGG 1 cut(s) 4598
NdeI CATATG 2 cut(s) 535, 3573
NgoMIV GCCGGC 1 cut(s) 251
NlaIV GGNNCC 7 cut(s) 706, 873, 914, 2214, 3061, 3175, 4060
NspI RCATGY 5 cut(s) 168, 801, 3661, 3745, 3832
NspV TTCGAA 1 cut(s) 139
PaqCI CACCTGC 1 cut(s) 3281
PciI ACATGT 3 cut(s) 3657, 3741, 3828
PciSI GCTCTTC 1 cut(s) 1337
PcsI WCGNNNNNNNCGW 3 cut(s) 363, 372, 486
PctI GAATGC 2 cut(s) 2323, 2350
PdiI GCCGGC 1 cut(s) 253
PdmI GAANNNNTTC 4 cut(s) 145, 2003, 3803, 3884
PflFI GACNNNGTC 1 cut(s) 92
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 9 cut(s) 44, 2227, 2398, 2813, 3571, 3655, 3739, 3826, 3907
PpsI GAGTC 9 cut(s) 44, 2227, 2398, 2813, 3571, 3655, 3739, 3826, 3907
PpuMI RGGWCCY 1 cut(s) 1442
PscI ACATGT 3 cut(s) 3657, 3741, 3828
PshAI GACNNNNGTC 5 cut(s) 2712, 3574, 3658, 3742, 3829
Psp124BI GAGCTC 3 cut(s) 60, 129, 179
Psp1406I AACGTT 1 cut(s) 144
Psp5II RGGWCCY 1 cut(s) 1442
Psp6I CCWGG 7 cut(s) 2486, 2621, 2759, 2897, 3020, 3413, 4475
PspFI CCCAGC 7 cut(s) 1230, 2288, 2667, 2805, 2943, 3279, 3297
PspGI CCWGG 7 cut(s) 2486, 2621, 2759, 2897, 3020, 3413, 4475
PspN4I GGNNCC 7 cut(s) 706, 873, 914, 2214, 3061, 3175, 4060
PspOMI GGGCCC 1 cut(s) 912
PspPI GGNCC 9 cut(s) 65, 912, 913, 1442, 1745, 2066, 2528, 4160, 4232
PspPPI RGGWCCY 1 cut(s) 1442
PsrI GAACNNNNNNTAC 2 cut(s) 825, 857
PstI CTGCAG 1 cut(s) 717
PstNI CAGNNNCTG 5 cut(s) 1702, 2609, 2639, 2885, 3005
PsuI RGATCY 4 cut(s) 610, 1622, 2212, 3173
PsyI GACNNNGTC 1 cut(s) 92
PvuI CGATCG 1 cut(s) 228
PvuII CAGCTG 2 cut(s) 2639, 3143
RgaI GCGATCGC 1 cut(s) 228
RseI CAYNNNNRTG 1 cut(s) 4350
SacI GAGCTC 3 cut(s) 60, 129, 179
SapI GCTCTTC 1 cut(s) 1337
SaqAI TTAA 8 cut(s) 1182, 1223, 1731, 2051, 2072, 2124, 2157, 2232
Sau96I GGNCC 9 cut(s) 65, 912, 913, 1442, 1745, 2066, 2528, 4160, 4232
ScaI AGTACT 1 cut(s) 3223
SchI GAGTC 9 cut(s) 45, 2228, 2399, 2813, 3572, 3656, 3740, 3827, 3908
SduI GDGCHC 9 cut(s) 60, 129, 179, 339, 916, 3206, 3590, 3674, 3758
SfaAI GCGATCGC 1 cut(s) 228
SfaNI GCATC 8 cut(s) 663, 2176, 2609, 2747, 2885, 2984, 3005, 3220
SfcI CTRYAG 3 cut(s) 417, 713, 1783
SfuI TTCGAA 1 cut(s) 139
SgfI GCGATCGC 1 cut(s) 228
SinI GGWCC 6 cut(s) 65, 1442, 1745, 2066, 4160, 4232
SmaI CCCGGG 1 cut(s) 2532
SmiMI CAYNNNNRTG 1 cut(s) 4350
SmlI CTYRAG 4 cut(s) 593, 1100, 1181, 3309
SmoI CTYRAG 4 cut(s) 593, 1100, 1181, 3309
SspI AATATT 1 cut(s) 1797
SstI GAGCTC 3 cut(s) 60, 129, 179
StyI CCWWGG 3 cut(s) 1982, 4598, 4682
TaiI ACGT 3 cut(s) 147, 789, 2466
TaqI TCGA 4 cut(s) 139, 206, 333, 480
TaqII GACCGA 1 cut(s) 2030
TatI WGTACW 2 cut(s) 1663, 3221
Tru1I TTAA 8 cut(s) 1182, 1223, 1731, 2051, 2072, 2124, 2157, 2232
Tru9I TTAA 8 cut(s) 1182, 1223, 1731, 2051, 2072, 2124, 2157, 2232
TscAI CASTG 7 cut(s) 67, 895, 1360, 2977, 3691, 3979, 4108
TspGWI ACGGA 2 cut(s) 2040, 3052
TspMI CCCGGG 1 cut(s) 2530
TspRI CASTG 7 cut(s) 67, 895, 1360, 2977, 3691, 3979, 4108
Tth111I GACNNNGTC 1 cut(s) 92
Vha464I CTTAAG 1 cut(s) 1181
VpaK11BI GGWCC 6 cut(s) 65, 1442, 1745, 2066, 4160, 4232
XagI CCTNNNNNAGG 2 cut(s) 1986, 3456
XapI RAATTY 6 cut(s) 135, 491, 1031, 1265, 1679, 2055
XceI RCATGY 5 cut(s) 168, 801, 3661, 3745, 3832
XcmI CCANNNNNNNNNTGG 1 cut(s) 1980
XmaI CCCGGG 1 cut(s) 2530
XmiI GTMKAC 2 cut(s) 161, 1782
XmnI GAANNNNTTC 4 cut(s) 145, 2003, 3803, 3884
ZrmI AGTACT 1 cut(s) 3223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.