RchiOBHm_Chr1g0362241

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012966
Physical Location & Seq
Reverse (-)
53912051 .. 53920591
8541 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58707

Sequence Viewer

Length: 3318 bp
ATGGCCTCGAAGGAAAAAGGGAAGGCGATCGCCACAAGCGGATGGTCCGCCGCCGGCAAGCGGAAGCCCGGCGATGATGACAAGTCCGGCGGCGGCGGTCGGAAGCGGAGAAACCCCGGCGCTCTCCAGTTCTTCGAGCACTCCGCCGCCGAAGCTGACGGTTCGGACGACGACAGCGACTTGGACGATTTTATGGAAGAAGAACTTGAAACAGAACCTATAGTCAAGAGTGAACCGGGGAAAGCCCATAACCTTCCATTTGTTCCCAAAGAGGAGGATATCGATGGGGACGAATTTGAAAGAATGATGGAAGAACGATACAGGACTGGTTCTACATATGTTACCTATGCTGAAGATAATTATGAAAACAAAAGATCGGTTGATGGAATTGTGCTTGAGCCTTCTGCTAAAGATCCTGTTATCTGGAAAGTAAAGTGTGCGGTTGGACGCGAGAGACACTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTACGATCAATGGGTACCAAGCTGCAGATAATATCTGCATTTGCTGTTGATCACATCAAGGGTTTTGTTTTCATTGAAGCTGATAAGCTATGTGACGTTCAGGAGGCATGTAAAGGACTTTGTAATATATTTTTATCCCGAGTAACGCCAGTTCCCAAAAATGAAGCTCCTCATTTACTGGCTCCTCGGACTAGAAACAGTGAAATTGCTGTGGGTACATGGGCCCGTGTGAAGAGTGGGAACTACAAGGGTGACTTGGCACAGGTTGTGGCTGTGAACGAAAAGAAAAAAGCAACAGTAAAGCTGATACCAAGAATTGATCTACAAGCAATGGCTGCAAAATTTGGTGGGGGAGTTACTAGAAAGAAACTGCCTACTCCAGCACCAAGATTGATCAGCACAAACGAACTTGAGGAGTTCCGCCCTCTCATTCAGGACAGGAAGGATAAGGATACCGGATTGCGTTTTCTCTGTCTTGATGGTTTGCTCCTTAAGGATGGATATTTATACAAAAAAATGCCATTAGATTCATTAAATTGCTGGGGTGTCATGCCATCAGAAGAGGAGATACTGAAATTCAAGCCTTCTGAGAACAATGAATCTGCTGATCTGGAGTGGCTTTCGCAGCTTTATGGTGACAATAAGAAAAGGAAGAGCATCGGCAGTGATAAAGGGGATGGCAAAGGAGAAGGTTCAGCAGGGTGTGGTGGGAAAGGAGAAGGTTCATCAAAGGGTAACGGGAAAGGAGAAGGTCCTTCAGGTGGGAAAGAAGGTTCTTCAGGGTTTGCTGGGAAAGGAGAAGGTTCTTCAGGGTGTGGTGGGAATCTCTATGAGCTGTATGATCTTGTTTGTTTCGGCAAGAAAGATTTTGGTCTTGTGCTAGGCATTGAGAAAGATGATACTTACAAGATTCTGAAAGAGGGTGTGGAAGGATCTACTGTTGTGACAGTTCAGCAGAATGAGATAAAGAATGTACTTTCTGATGTAAAATTTACAGCTTTAGATCAGCGCCTGAAGCCCATTTGCGTCAATGATACAGTTAAAGTTTTGGAAGGACCATTGAAGGATAGGCAAGGAATAGTGAAGCTAGTCTACAGAGGCACAATATTCATGTTTGATGAGAATGAGACAGAAAATGGTGGTTATTTCTGCTCAAAATCTCAAATGTGTGAGAAAATCAAGCTTTCCATTGACGTTATCCCAGAAAAGGATGGGGATTCGGGTGCTTTCGGTTTTGAAGATTTCACATCATCACCTAAATCCCCTCTATCGCCAAAAAAGCCTTGGCTAGGAAGGGAGAATAACTTCAATCAGGGCGATACTGACGGAATGTTCTCAATCGGTCAAACTTTAAGAATTCGTGTTGGTCCATTAAAGGGATACCTCTGTCGTGTCTTGGCCATACGCCGTTCTGATATCACAGTTAAGCTTGATTCTCAGCAAAGGGTTCTTACAGTTAAAGCTGAGCATCTTACAGAGGTTCGTGTAAAGAGCGCTGCCATGCTTAGTGAGGATCCGGAGTCCAGCTCTCTTAAACCATTTGATTTACTTGGAACTGAAGGAGGCTCTAAAGATTGGACAGATGGAGCTGGGACATCAGCCGGGGGTGATGGGTGGAATGTTGGAGGGTCATCTGGTGAAAGGAATGCTTGGCCTAGTTTCTCTGCCTCGGGCAATTTGCTTCAGCCTGAGTCCAGCTCTGCGAATCCTTTTGAATCTGATGGAAATGGTGCTAAAAAAGAGGATAGTTCTTGGGAGAGGAACGTGGCTCCAAATAACAACTCATCCTGGGGTGTTGCAGCAGCAGAAAACAAGGATCAAGTTGCTGGCCGGGGAAAAAGCAACTCTTGGGGCACTAAAGTTGGAGATGACAGCACTTCGTCAGATACTTGGCAAAAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGCAGCAGCTGTAAATAACAAGGATCAAGGTAATGGCTGGGGAAAAAGCGATTCTTGGGGCGCTAAAGTTGGAGGTGACAGCAGTCTGTCAGATACTTTGCAAAAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGCAGCAGCAGTAGATAACAAGGATCAAGGTACTGACTGGGGAAAAAGTGACTCTTGGGGCGCTAAAGTTGGAGGTGACAGCACTTCGTCGGATACTTGGCAAAAAGCATCAGCGCCTGCTAGTTCATCCTGGGGTGTTGCGGCAGCAATAGATAAGAAGGATCAAGGTATTGGCTGGGGTGGTAAGACTGGATGTGACGGCACTGCATCAGATAGTTGGAAAAAAGCATCAGAACCTGCTAGTAATTCGTCCTGGGGTGTTGCAGCAGCAGCAGATAACAAGGATCAAGGTTCCGTCTGGGGCGGTAGTGGTAGTCAAAAACCGAGTGTTGCAGATGATAGTGGTGACAAAGGTTCTGCTTGGAATAAGCCAGCTGAAGGTTCTTGGAGTAAACAAACTGGAGGATCCTCATGGGGTAAGCAAGCCGATTGGAGCACTAAAGAGGGATCAAGTACTGGTGATGCAGGGTGGAAAAGTTCAACGCCTTCAGTAGAGAATCAGACTGGGAGCAGGGGTAATGCAGGTGGCTGGGCAAAAGCTCAAGGCATATCTGGTAATGAGAATCATAAAGACAGTTGGAAGAAACCAAGTGGTATAGATGACAATAAAAATGCTTCGTGGATCAAGGAAGATGGAGGTTCTACCTGGAATAAACAAGATGGAGGTTCTACTTGGAATAAGCCTGCTGGAGGTTCTTGGAGTAAAGGTGGGGATCAAGGGTCTGGTGGTGGCTATGGTGGGTGGTGGTGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1105

Amino Acids

117.96

Weight (kDa)

5.5

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 141 - 224 1.2e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 234 - 357 3.7e-26 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 438 - 488 2.4e-10 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 497 - 551 5.9e-21 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 611 - 655 1.4e-06 Transcription elongation factor SPT5, fifth KOW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3074
Acc36I ACCTGC 4 cut(s) 2410, 2548, 2806, 3074
Acc65I GGTACC 1 cut(s) 506
AccB1I GGYRCC 1 cut(s) 506
AccI GTMKAC 1 cut(s) 1584
AccII CGCG 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 2008
AcoI YGGCCR 2 cut(s) 1890, 2320
AcsI RAATTY 5 cut(s) 293, 833, 1067, 1481, 1848
AcuI CTGAAG 9 cut(s) 372, 1233, 1254, 1284, 1526, 2070, 2159, 2958, 3033
AfaI GTAC 5 cut(s) 508, 709, 1467, 2593, 3016
AfeI AGCGCT 1 cut(s) 1987
AfiI CCNNNNNNNGG 7 cut(s) 60, 1253, 1699, 1781, 1868, 2939, 3251
AflII CTTAAG 1 cut(s) 983
AgsI TTSAA 9 cut(s) 209, 299, 569, 1072, 1555, 1730, 1801, 2207, 3042
AjnI CCWGG 6 cut(s) 2279, 2414, 2552, 2690, 2813, 3206
AloI GAACNNNNNNTCC 2 cut(s) 1249, 1281
Alw21I GWGCWC 2 cut(s) 141, 2999
Alw26I GTCTC 2 cut(s) 448, 1613
AlwNI CAGNNNCTG 6 cut(s) 1504, 2402, 2432, 2540, 2678, 2798
Ama87I CYCGRG 2 cut(s) 630, 2161
Aor13HI TCCGGA 1 cut(s) 2008
Aor51HI AGCGCT 1 cut(s) 1987
AoxI GGCC 5 cut(s) 3, 714, 1890, 2144, 2320
ApaI GGGCCC 1 cut(s) 718
ApoI RAATTY 5 cut(s) 293, 833, 1067, 1481, 1848
AsiSI GCGATCGC 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 1796
Asp718I GGTACC 1 cut(s) 506
AspLEI GCGC 6 cut(s) 122, 1503, 1988, 2486, 2624, 2677
AspS9I GGNCC 6 cut(s) 45, 714, 715, 1244, 1547, 1859
AsuC2I CCSGG 5 cut(s) 69, 117, 237, 2095, 2324
AsuHPI GGTGA 9 cut(s) 755, 1139, 1737, 2111, 2141, 2510, 2648, 2918, 3032
AvaI CYCGRG 2 cut(s) 630, 2161
AvaII GGWCC 4 cut(s) 45, 1244, 1547, 1859
BaeGI GKGCMC 2 cut(s) 718, 2348
BaeI ACNNNNGTAYC 2 cut(s) 1864, 1897
BalI TGGCCA 1 cut(s) 1892
BamHI GGATCC 2 cut(s) 2005, 2966
BanI GGYRCC 1 cut(s) 506
BanII GRGCYC 1 cut(s) 718
BarI GAAGNNNNNNTAC 4 cut(s) 1044, 1076, 1568, 1600
Bbv12I GWGCWC 2 cut(s) 141, 2999
BceAI ACGGC 2 cut(s) 1884, 2776
BciT130I CCWGG 6 cut(s) 2281, 2416, 2554, 2692, 2815, 3208
BciVI GTATCC 3 cut(s) 937, 1865, 2647
BclI TGATCA 2 cut(s) 541, 885
BcnI CCSGG 5 cut(s) 69, 117, 237, 2095, 2324
BcoDI GTCTC 2 cut(s) 448, 1613
BfaI CTAG 8 cut(s) 684, 852, 1373, 1580, 1781, 2148, 2682, 2802
BfmI CTRYAG 3 cut(s) 219, 515, 1585
BfoI RGCGCY 6 cut(s) 123, 1504, 1989, 2487, 2625, 2678
BfrI CTTAAG 1 cut(s) 983
BfuAI ACCTGC 4 cut(s) 2410, 2548, 2806, 3074
BfuI GTATCC 3 cut(s) 937, 1865, 2647
BlpI GCTNAGC 1 cut(s) 1956
BmcAI AGTACT 1 cut(s) 3016
Bme18I GGWCC 4 cut(s) 45, 1244, 1547, 1859
BmeT110I CYCGRG 2 cut(s) 630, 2161
BmgT120I GGNCC 6 cut(s) 45, 714, 715, 1244, 1547, 1859
BmiI GGNNCC 7 cut(s) 508, 675, 716, 2007, 2262, 2854, 2968
BmrI ACTGGG 2 cut(s) 2608, 3075
BmsI GCATC 9 cut(s) 465, 1158, 1969, 2402, 2540, 2678, 2777, 2798, 3013
BmuI ACTGGG 2 cut(s) 2608, 3075
BoxI GACNNNNGTC 1 cut(s) 2505
BplI GAGNNNNNCTC 6 cut(s) 443, 475, 2003, 2035, 2174, 2206
BpmI CTGGAG 5 cut(s) 110, 855, 1124, 2982, 3270
Bpu1102I GCTNAGC 1 cut(s) 1956
BpuEI CTTGAG 3 cut(s) 416, 923, 3087
BpuMI CCSGG 5 cut(s) 69, 117, 237, 2095, 2324
Bsa29I ATCGAT 1 cut(s) 282
BsaWI WCCGGW 2 cut(s) 947, 2008
BsaXI ACNNNNNCTCC 4 cut(s) 3061, 3091, 3253, 3283
Bsc4I CCNNNNNNNGG 7 cut(s) 60, 1253, 1699, 1781, 1868, 2939, 3251
Bse118I RCCGGY 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 828
BseAI TCCGGA 1 cut(s) 2008
BseBI CCWGG 6 cut(s) 2281, 2416, 2554, 2692, 2815, 3208
BseCI ATCGAT 1 cut(s) 282
BseGI GGATG 9 cut(s) 47, 994, 1174, 1708, 2276, 2411, 2549, 2687, 2759
BseLI CCNNNNNNNGG 7 cut(s) 60, 1253, 1699, 1781, 1868, 2939, 3251
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 5 cut(s) 474, 1071, 1943, 1947, 2172
BseRI GAGGAG 5 cut(s) 287, 651, 666, 920, 1070
BseSI GKGCMC 2 cut(s) 718, 2348
BseYI CCCAGC 6 cut(s) 1032, 1280, 2081, 2460, 2736, 3090
Bsh1236I CGCG 1 cut(s) 450
Bsh1285I CGRYCG 2 cut(s) 30, 100
BshFI GGCC 5 cut(s) 5, 716, 1892, 2146, 2322
BshNI GGYRCC 1 cut(s) 506
BshVI ATCGAT 1 cut(s) 282
BsiEI CGRYCG 2 cut(s) 30, 100
BsiHKAI GWGCWC 2 cut(s) 141, 2999
BsiHKCI CYCGRG 2 cut(s) 630, 2161
BsiSI CCGG 9 cut(s) 54, 69, 87, 117, 236, 948, 2009, 2094, 2323
BslFI GGGAC 2 cut(s) 302, 2098
BslI CCNNNNNNNGG 7 cut(s) 60, 1253, 1699, 1781, 1868, 2939, 3251
BsmAI GTCTC 2 cut(s) 448, 1613
BsmFI GGGAC 2 cut(s) 302, 2098
BsmI GAATGC 1 cut(s) 2143
BsnI GGCC 5 cut(s) 5, 716, 1892, 2146, 2322
BsoBI CYCGRG 2 cut(s) 630, 2161
Bsp120I GGGCCC 1 cut(s) 714
Bsp1286I GDGCHC 4 cut(s) 141, 718, 2348, 2999
Bsp13I TCCGGA 1 cut(s) 2008
Bsp1720I GCTNAGC 1 cut(s) 1956
BspANI GGCC 5 cut(s) 5, 716, 1892, 2146, 2322
BspCNI CTCAG 5 cut(s) 473, 1072, 1942, 1948, 2173
BspDI ATCGAT 1 cut(s) 282
BspEI TCCGGA 1 cut(s) 2008
BspFNI CGCG 1 cut(s) 450
BspLI GGNNCC 7 cut(s) 508, 675, 716, 2007, 2262, 2854, 2968
BspMAI CTGCAG 1 cut(s) 519
BspMI ACCTGC 4 cut(s) 2410, 2548, 2806, 3074
BspQI GCTCTTC 1 cut(s) 1139
BspT107I GGYRCC 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 983
BsrDI GCAATG 1 cut(s) 828
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 1775
Bst2UI CCWGG 6 cut(s) 2281, 2416, 2554, 2692, 2815, 3208
Bst4CI ACNGT 9 cut(s) 161, 692, 790, 1432, 1441, 1531, 1915, 1948, 3137
Bst6I CTCTTC 3 cut(s) 719, 1047, 1139
BstAFI CTTAAG 1 cut(s) 983
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 7 cut(s) 55, 59, 2320, 2679, 2934, 2985, 3246
BstDEI CTNAG 6 cut(s) 460, 1080, 1929, 1956, 1997, 2181
BstENI CCTNNNNNAGG 2 cut(s) 1779, 3249
BstF5I GGATG 9 cut(s) 47, 994, 1174, 1708, 2276, 2411, 2549, 2687, 2759
BstFNI CGCG 1 cut(s) 450
BstH2I RGCGCY 6 cut(s) 123, 1504, 1989, 2487, 2625, 2678
BstHHI GCGC 6 cut(s) 122, 1503, 1988, 2486, 2624, 2677
BstMAI GTCTC 2 cut(s) 448, 1613
BstMCI CGRYCG 2 cut(s) 30, 100
BstMWI GCNNNNNNNGC 6 cut(s) 152, 827, 1117, 1507, 1771, 2831
BstNI CCWGG 6 cut(s) 2281, 2416, 2554, 2692, 2815, 3208
BstNSI RCATGY 1 cut(s) 603
BstPAI GACNNNNGTC 1 cut(s) 2505
BstSFI CTRYAG 3 cut(s) 219, 515, 1585
BstSLI GKGCMC 2 cut(s) 718, 2348
BstUI CGCG 1 cut(s) 450
BstX2I RGATCY 4 cut(s) 412, 1424, 2005, 2966
BstYI RGATCY 4 cut(s) 412, 1424, 2005, 2966
Bsu15I ATCGAT 1 cut(s) 282
BsuI GTATCC 3 cut(s) 937, 1865, 2647
BsuRI GGCC 5 cut(s) 5, 716, 1892, 2146, 2322
BsuTUI ATCGAT 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 9 cut(s) 47, 994, 1174, 1708, 2276, 2411, 2549, 2687, 2759
BtsI GCAGTG 2 cut(s) 1162, 2763
BtsIMutI CAGTG 3 cut(s) 697, 1162, 2763
BveI ACCTGC 4 cut(s) 2410, 2548, 2806, 3074
Cac8I GCNNGC 7 cut(s) 55, 59, 2320, 2679, 2934, 2985, 3246
CaiI CAGNNNCTG 6 cut(s) 1504, 2402, 2432, 2540, 2678, 2798
CfoI GCGC 6 cut(s) 122, 1503, 1988, 2486, 2624, 2677
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 6 cut(s) 45, 714, 715, 1244, 1547, 1859
ClaI ATCGAT 1 cut(s) 282
CseI GACGC 2 cut(s) 456, 1507
Csp6I GTAC 5 cut(s) 507, 708, 1466, 2592, 3015
CviAII CATG 6 cut(s) 600, 711, 1042, 1603, 1993, 2973
CviQI GTAC 5 cut(s) 507, 708, 1466, 2592, 3015
DdeI CTNAG 6 cut(s) 460, 1080, 1929, 1956, 1997, 2181
EaeI YGGCCR 2 cut(s) 1890, 2320
Eam1104I CTCTTC 3 cut(s) 719, 1047, 1139
EarI CTCTTC 3 cut(s) 719, 1047, 1139
EciI GGCGGA 3 cut(s) 37, 133, 902
Eco130I CCWWGG 1 cut(s) 1775
Eco24I GRGCYC 1 cut(s) 718
Eco32I GATATC 2 cut(s) 280, 1909
Eco47I GGWCC 4 cut(s) 45, 1244, 1547, 1859
Eco47III AGCGCT 1 cut(s) 1987
Eco57I CTGAAG 9 cut(s) 372, 1233, 1254, 1284, 1526, 2070, 2159, 2958, 3033
Eco88I CYCGRG 2 cut(s) 630, 2161
EcoNI CCTNNNNNAGG 2 cut(s) 1779, 3249
EcoO109I RGGNCCY 1 cut(s) 1244
EcoRI GAATTC 1 cut(s) 1848
EcoRII CCWGG 6 cut(s) 2279, 2414, 2552, 2690, 2813, 3206
EcoRV GATATC 2 cut(s) 280, 1909
EcoT14I CCWWGG 1 cut(s) 1775
EcoT38I GRGCYC 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 1775
FaeI CATG 6 cut(s) 603, 714, 1045, 1606, 1996, 2976
FaqI GGGAC 2 cut(s) 302, 2098
FatI CATG 6 cut(s) 599, 710, 1041, 1602, 1992, 2972
FauNDI CATATG 1 cut(s) 337
FbaI TGATCA 2 cut(s) 541, 885
FblI GTMKAC 1 cut(s) 1584
FokI GGATG 9 cut(s) 54, 1001, 1181, 1715, 2263, 2398, 2536, 2674, 2766
FriOI GRGCYC 1 cut(s) 718
FspBI CTAG 8 cut(s) 684, 852, 1373, 1580, 1781, 2148, 2682, 2802
GlaI GCGC 6 cut(s) 121, 1502, 1987, 2485, 2623, 2676
GsaI CCCAGC 6 cut(s) 1036, 1284, 2085, 2464, 2740, 3094
GsuI CTGGAG 5 cut(s) 110, 855, 1124, 2982, 3270
HaeII RGCGCY 6 cut(s) 123, 1504, 1989, 2487, 2625, 2678
HaeIII GGCC 5 cut(s) 5, 716, 1892, 2146, 2322
HapII CCGG 9 cut(s) 54, 69, 87, 117, 236, 948, 2009, 2094, 2323
HgaI GACGC 2 cut(s) 456, 1507
HhaI GCGC 6 cut(s) 122, 1503, 1988, 2486, 2624, 2677
Hin1II CATG 6 cut(s) 603, 714, 1045, 1606, 1996, 2976
Hin6I GCGC 6 cut(s) 120, 1501, 1986, 2484, 2622, 2675
HinP1I GCGC 6 cut(s) 120, 1501, 1986, 2484, 2622, 2675
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 2 cut(s) 1673, 1919
HpaII CCGG 9 cut(s) 54, 69, 87, 117, 236, 948, 2009, 2094, 2323
HphI GGTGA 9 cut(s) 755, 1139, 1737, 2111, 2141, 2510, 2648, 2918, 3032
Hpy166II GTNNAC 5 cut(s) 233, 490, 769, 1585, 2954
Hpy188III TCNNGA 8 cut(s) 226, 424, 593, 630, 926, 968, 1103, 2009
Hpy8I GTNNAC 5 cut(s) 233, 490, 769, 1585, 2954
Hpy99I CGWCG 2 cut(s) 173, 2653
HpyCH4III ACNGT 9 cut(s) 161, 692, 790, 1432, 1441, 1531, 1915, 1948, 3137
HpyCH4IV ACGT 3 cut(s) 588, 1686, 2256
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 827, 1117, 1507, 1771, 2831
HpyF3I CTNAG 6 cut(s) 460, 1080, 1929, 1956, 1997, 2181
HpySE526I ACGT 3 cut(s) 588, 1686, 2256
Hsp92II CATG 6 cut(s) 603, 714, 1045, 1606, 1996, 2976
HspAI GCGC 6 cut(s) 120, 1501, 1986, 2484, 2622, 2675
Kpn2I TCCGGA 1 cut(s) 2008
KpnI GGTACC 1 cut(s) 510
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Ksp22I TGATCA 2 cut(s) 541, 885
LguI GCTCTTC 1 cut(s) 1139
LmnI GCTCC 7 cut(s) 664, 679, 984, 2078, 2266, 2994, 3069
LweI GCATC 9 cut(s) 465, 1158, 1969, 2402, 2540, 2678, 2777, 2798, 3013
MaeI CTAG 8 cut(s) 684, 852, 1373, 1580, 1781, 2148, 2682, 2802
MaeII ACGT 3 cut(s) 588, 1686, 2256
MflI RGATCY 4 cut(s) 412, 1424, 2005, 2966
MhlI GDGCHC 4 cut(s) 141, 718, 2348, 2999
MlsI TGGCCA 1 cut(s) 1892
MluNI TGGCCA 1 cut(s) 1892
MlyI GAGTC 3 cut(s) 2021, 2192, 2606
MmeI TCCRAC 9 cut(s) 80, 424, 2095, 2335, 2473, 2611, 2631, 2759, 3119
Mox20I TGGCCA 1 cut(s) 1892
MroI TCCGGA 1 cut(s) 2008
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 1796
MscI TGGCCA 1 cut(s) 1892
MseI TTAA 8 cut(s) 984, 1025, 1533, 1844, 1865, 1917, 1950, 2025
Msp20I TGGCCA 1 cut(s) 1892
MspA1I CMGCKG 2 cut(s) 2432, 2936
MspCI CTTAAG 1 cut(s) 983
MspI CCGG 9 cut(s) 54, 69, 87, 117, 236, 948, 2009, 2094, 2323
Mva1269I GAATGC 1 cut(s) 2143
MvaI CCWGG 6 cut(s) 2281, 2416, 2554, 2692, 2815, 3208
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 6 cut(s) 152, 827, 1117, 1507, 1771, 2831
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 5 cut(s) 69, 117, 237, 2095, 2324
NdeI CATATG 1 cut(s) 337
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 6 cut(s) 603, 714, 1045, 1606, 1996, 2976
NlaIV GGNNCC 7 cut(s) 508, 675, 716, 2007, 2262, 2854, 2968
NmuCI GTSAC 9 cut(s) 584, 743, 1127, 1435, 2498, 2609, 2636, 2756, 2906
NspI RCATGY 1 cut(s) 603
PaqCI CACCTGC 1 cut(s) 3074
PciSI GCTCTTC 1 cut(s) 1139
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 174, 288
PctI GAATGC 1 cut(s) 2143
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 1796
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 3 cut(s) 2020, 2191, 2606
PpsI GAGTC 3 cut(s) 2020, 2191, 2606
PpuMI RGGWCCY 1 cut(s) 1244
PshAI GACNNNNGTC 1 cut(s) 2505
Psp5II RGGWCCY 1 cut(s) 1244
Psp6I CCWGG 6 cut(s) 2279, 2414, 2552, 2690, 2813, 3206
PspFI CCCAGC 6 cut(s) 1032, 1280, 2081, 2460, 2736, 3090
PspGI CCWGG 6 cut(s) 2279, 2414, 2552, 2690, 2813, 3206
PspN4I GGNNCC 7 cut(s) 508, 675, 716, 2007, 2262, 2854, 2968
PspOMI GGGCCC 1 cut(s) 714
PspPI GGNCC 6 cut(s) 45, 714, 715, 1244, 1547, 1859
PspPPI RGGWCCY 1 cut(s) 1244
PsrI GAACNNNNNNTAC 2 cut(s) 627, 659
PstI CTGCAG 1 cut(s) 519
PstNI CAGNNNCTG 6 cut(s) 1504, 2402, 2432, 2540, 2678, 2798
PsuI RGATCY 4 cut(s) 412, 1424, 2005, 2966
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 2 cut(s) 2432, 2936
RgaI GCGATCGC 1 cut(s) 30
RsaI GTAC 5 cut(s) 508, 709, 1467, 2593, 3016
RsaNI GTAC 5 cut(s) 507, 708, 1466, 2592, 3015
SapI GCTCTTC 1 cut(s) 1139
SaqAI TTAA 8 cut(s) 984, 1025, 1533, 1844, 1865, 1917, 1950, 2025
Sau96I GGNCC 6 cut(s) 45, 714, 715, 1244, 1547, 1859
ScaI AGTACT 1 cut(s) 3016
SchI GAGTC 3 cut(s) 2021, 2192, 2606
SduI GDGCHC 4 cut(s) 141, 718, 2348, 2999
SfaAI GCGATCGC 1 cut(s) 30
SfaNI GCATC 9 cut(s) 465, 1158, 1969, 2402, 2540, 2678, 2777, 2798, 3013
SfcI CTRYAG 3 cut(s) 219, 515, 1585
SgfI GCGATCGC 1 cut(s) 30
SinI GGWCC 4 cut(s) 45, 1244, 1547, 1859
SmlI CTYRAG 4 cut(s) 395, 902, 983, 3102
SmoI CTYRAG 4 cut(s) 395, 902, 983, 3102
SspI AATATT 1 cut(s) 1599
SspMI CTAG 8 cut(s) 684, 852, 1373, 1580, 1781, 2148, 2682, 2802
StyI CCWWGG 1 cut(s) 1775
TaaI ACNGT 9 cut(s) 161, 692, 790, 1432, 1441, 1531, 1915, 1948, 3137
TaiI ACGT 3 cut(s) 591, 1689, 2259
TaqI TCGA 3 cut(s) 8, 135, 282
TaqII GACCGA 1 cut(s) 1823
TatI WGTACW 2 cut(s) 1465, 3014
TauI GCSGC 5 cut(s) 53, 93, 96, 149, 2705
Tru1I TTAA 8 cut(s) 984, 1025, 1533, 1844, 1865, 1917, 1950, 2025
Tru9I TTAA 8 cut(s) 984, 1025, 1533, 1844, 1865, 1917, 1950, 2025
TscAI CASTG 3 cut(s) 697, 1162, 2770
TseFI GTSAC 9 cut(s) 584, 743, 1127, 1435, 2498, 2609, 2636, 2756, 2906
Tsp45I GTSAC 9 cut(s) 584, 743, 1127, 1435, 2498, 2609, 2636, 2756, 2906
TspGWI ACGGA 2 cut(s) 1833, 2845
TspRI CASTG 3 cut(s) 697, 1162, 2770
Vha464I CTTAAG 1 cut(s) 983
VpaK11BI GGWCC 4 cut(s) 45, 1244, 1547, 1859
XagI CCTNNNNNAGG 2 cut(s) 1779, 3249
XapI RAATTY 5 cut(s) 293, 833, 1067, 1481, 1848
XceI RCATGY 1 cut(s) 603
XcmI CCANNNNNNNNNTGG 1 cut(s) 1773
XmiI GTMKAC 1 cut(s) 1584
XmnI GAANNNNTTC 1 cut(s) 1796
XspI CTAG 8 cut(s) 684, 852, 1373, 1580, 1781, 2148, 2682, 2802
ZrmI AGTACT 1 cut(s) 3016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.